Pesticide composition containing lepicortin

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing lepicortin. The pesticide composition containing the lepicortin is formed by compounding the lepicortin and a second active component, and the second active component is cyprofluzamide or tetratolfenpyrad. According to the invention, the lepimectin is compounded with the cyproflufen or the tolfenpyrad, so that a synergistic interaction effect is achieved, the prevention and treatment effect is improved, and the usage amount of a medicament can be reduced. The lepimectin and the cyproflufen or the tetratofenpyrad have different action mechanisms, so that the generation and accumulation of drug resistance and drug resistance can be effectively delayed, and the drug resistance risk of the medicament is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide control, and particularly relates to a pesticide composition containing lepimectin. Background Art

[0002] Lepimectin is a 16-membered macrolide antibiotic insecticide that acts on glutamate-gated chloride channels. - ). As a glutamate agonist, lepidomectin can increase Glu Cl - The opening frequency of the membrane leads to the - The permeability increases, causing the hyperpolarization of the resting potential of the neuron, making it difficult to depolarize, blocking nerve conduction, and then leading to flaccid paralysis of the insect body wall machine head, blocking the peristalsis of the insect pharyngeal muscles, causing difficulty in feeding and leading to death. As a pesticide insecticide, lepimectin can be safely used on a variety of vegetables and fruits such as cabbage, tomatoes, citrus, strawberries and grapes. Its insecticide spectrum is also relatively wide, and most lepidopteran and homopteran pests such as Spodoptera litura, diamondback moth, cotton bollworm and mealybugs have a good repelling effect, and lepimectin has the advantages of low residual amount, fast degradation, low toxicity and high safety. Although lepimectin has the above advantages, as a single insecticide active ingredient, long-term use is easy to make pests resistant to drugs, resulting in reduced prevention effect. Compounding lepimectin with other active ingredients can effectively solve the above problems, so it is of great significance to study the composite composition of lepimectin.

[0003] Cyproflanilide is a diamide insecticide developed by Nantong Taihe on the basis of diamide insecticides. It is an allosteric modulator of γ-aminobutyric acid (GABA)-gated chloride ion channels. It blocks activated γ-aminobutyric acid chloride ion channels by allosteric blocking, causing pests to become overexcited and spasm, eventually leading to their death. Although cyproflanilide has a diamide structure, it is not a traditional nicotinic acid receptor modulator. It is classified as Group 30 in the IRAC classification of bromofenac. Cyproflanilide can effectively control lepidopteran pests such as beet armyworm, diamondback moth, armyworm and striped stem borer, coleopteran pests such as longhorn beetles and leaf beetles, and thysanopteran pests such as thrips.

[0004] Tetraniliprole is an o-formylaminobenzamide insecticide developed by Bayer. It is a muscle poison that acts on insect nicotinic acid receptors. Nicotinic acid receptors are selective ion channels that regulate the orderly release of intracellular calcium ions, that is, tetrameric channel proteins that regulate the balance of intracellular calcium ion concentration. After tetrazolin binds to insect nicotinic acid receptors with high affinity, the calcium ion release channel will continue to open, causing the calcium ions stored in smooth muscle and striated muscle cells to be released out of control and lost, resulting in paralysis of muscle cell contraction function, thereby achieving the purpose of killing insects. Tetraniliprole can be used to control various types of pests such as Lepidoptera, Coleoptera, Hemiptera, Thysanoptera, Diptera and Orthoptera. Summary of the invention

[0005] The object of the present invention is to provide a pesticide composition containing lepimectin to solve the problem that lepimectin, as a single insecticidal active ingredient, can easily cause pests to develop resistance to the pesticide after long-term use, resulting in reduced control efficacy.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A pesticide composition containing lepimectin is prepared by compounding lepimectin with a second active ingredient, wherein the second active ingredient is cyflumifenamide or tetrazopyrad.

[0008] Furthermore, the mass ratio of lepidomicil to ciprofloxacin is 1-9:18-1.

[0009] Furthermore, the mass ratio of lepimectin to ciprofloxacin is 1:18, 1:9, 1:3, 1:1, 3:1 or 9:1.

[0010] Furthermore, the mass ratio of the lepidomicil to the tetrazobactam is 1-4:4-1.

[0011] Furthermore, the mass ratio of lepidomicil to tetrazobactam is 1:4, 1:2, 1:1, 2:1 or 4:1.

[0012] A pesticide preparation, comprising the pesticide composition containing lepimectin.

[0013] Furthermore, the mass of the lepimectin-containing pesticide composition accounts for 0.01-40% of the total mass of the pesticide preparation.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The compounding of lepidomicil with cyflumizone or tetrazopyram has a synergistic effect, which not only helps to improve the control effect, but also helps to reduce the amount of the agent used.

[0016] (2) The lepidomicil of the present invention has a different mechanism of action from cyfluthrin or tetrazobactam, and can effectively delay the generation and accumulation of drug resistance and reduce the risk of drug resistance. DETAILED DESCRIPTION

[0017] In order to better understand the essence of the present invention, the present invention is further described in detail below in conjunction with embodiments.

[0018] Indoor biological activity test of lepidomicil and cyprodinil or tetrazolylpyrad

[0019] 1. Test reagents

[0020] 99% lepidomicil (Shanghai Titan Technology Co., Ltd.), 98% cyprodinil (Nantong Taihe Chemical Co., Ltd.), 90% tetrazobactam (Bayer AG)

[0021] 2. Test pests

[0022] Frankliniella occidentalis was collected from a strawberry plantation and reared for four generations using fresh and clean bean pods (without seeds) in an artificial climate chamber at a temperature of 26±1°C, a light intensity of 16L / 8D, and a relative humidity of (65±5)%. Adults with the same physiological state were selected as test subjects. During the rearing of Frankliniella occidentalis, beans were replaced every 3 days.

[0023] 3. Test methods

[0024] The toxicity of single and mixed agents to western flower thrips was determined by the immersion method. The test agent was dissolved in dimethyl sulfoxide, and then diluted with distilled water containing 0.01% Triton X-100 to a single agent stock solution with an active ingredient concentration of 10000 mg / L; multiple groups of ratios were set up, and each single agent and each group of mixed agents were diluted with distilled water containing 0.01% Triton X-100 to prepare 7 mass concentration gradients, each mass concentration solution 50mL, and set aside.

[0025] Remove the seeds from fresh and clean bean pods, soak them in the liquid for 20 seconds, take them out and dry them, and place them in a transparent insect breeding box (200 mm long, 10 mm wide, and 5 mm high). Punch several small holes on the lid of the breeding box, and the hole diameter is determined to prevent the test pests from escaping. Place 3 bean pods in each breeding box. Then, inoculate 20 western flower thrips adults in each breeding box, cover the box lid, and place it in an artificial climate box with a temperature of 26±1℃, a light of 16L / 8D, and a relative humidity of (65±5)%. Set the bean pods treated with 0.01% Triton X-100 diluted in distilled water as a blank control. Each breeding box is replicated once, and each treatment is replicated four times.

[0026] After 48 hours, the number of deaths, survivals and total number of western flower thrips were investigated and recorded in detail (the insects that could not crawl when touched by the tip of a brush were considered dead), and the corrected mortality rate of each treatment was calculated based on the investigation results. The DPS software was used to perform regression analysis on the logarithmic value of the concentration of the pesticide and the corrected mortality probability value, and the regression equation of each treatment pesticide and LC were calculated. 50 .

[0027]

[0028] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0029]

[0030] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0031] 5. Pharmaceutical evaluation

[0032] The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC value) of the mixture, and the joint effect was divided according to the standard: co-toxicity coefficient (CTC) ≥ 120 showed synergistic effect; co-toxicity coefficient (CTC) ≤ 80 showed antagonistic effect; 80 < co-toxicity coefficient (CTC) < 120 showed additive effect. The results are shown in Table 1-2.

[0033] Table 1 Toxicity of the combination of lepidomycin and ciprofloxacin to western flower thrips

[0034]

[0035] From the results in Table 1, it can be seen that when lepidomicil and ciprofloxacin are compounded in a mass ratio of 1-9:18-1, the co-toxicity coefficient to western flower thrips is greater than 120, which has a synergistic effect.

[0036] Table 2 Toxicity of lepidomicil and tetrazobactam to western flower thrips

[0037]

[0038] From the results in Table 2, it can be seen that when lepidomicil and tetrazobactam are compounded in a mass ratio of 1-4:4-1, the co-toxicity coefficient to western flower thrips is greater than 120, which has a synergistic effect.

[0039] From Table 1 and Table 2, it can be seen that the compounding of lepidomicil with cyfluthrin or tetrazobactam has a synergistic effect, which not only helps to improve the control effect, but also helps to reduce the usage of the agent.

Claims

1. A pesticide composition containing lepimectin, characterized in that: The invention is prepared by compounding lepidomicil with a second active ingredient, wherein the second active ingredient is cyprodinil or tetrazopyrid.

2. The pesticide composition containing lepimectin according to claim 1, characterized in that: The mass ratio of lepimectin to ciprofloxacin is 1-9:18-1.

3. The pesticide composition containing lepimectin according to claim 2, characterized in that: The mass ratio of lepidomicil to ciprofloxacin is 1:18, 1:9, 1:3, 1:1, 3:1 or 9:

1.

4. The pesticide composition containing lepimectin according to claim 1, characterized in that: The mass ratio of the lepidomicil to the tetrazobactam is 1-4:4-1.

5. The pesticide composition containing lepimectin according to claim 1, characterized in that: The mass ratio of the lepidomicil to the tetrazobactam is 1:4, 1:2, 1:1, 2:1 or 4:

1.

6. A pesticide preparation, characterized in that: The pesticide preparation comprises the pesticide composition containing lepimectin according to any one of claims 1 to 5.

7. The pesticide formulation according to claim 6, characterized in that: The mass of the lepimectin-containing pesticide composition accounts for 0.01-40% of the total mass of the pesticide preparation.

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