Application of pitaya probiotic composition in rapidly promoting defecation and maintaining stability of intestinal flora

Abstract

The invention discloses application of a probiotic composition in rapidly promoting defecation and maintaining intestinal flora stability, and belongs to the field of probiotic supplements. The invention provides a composition with specific components and formula, the composition can quickly promote defecation of adults within 24 hours under the action of probiotics, water-soluble dietary fibers and pitaya powder, and the problem of constipation of patients is quickly solved; meanwhile, the medicine is high in safety, does not contain laxative components, and avoids stimulating intestinal tracts; the three probiotics can balance intestinal flora, so that a patient can keep long-term regular defecation. The bamboo extract powder is added in the preparation process of the powder, so that the flowability of the powder can be improved, and all the components are mixed more uniformly.

Description

Technical Field

[0001] The present invention relates to the field of probiotic supplements, and in particular to use of a probiotic composition in rapidly promoting defecation and maintaining the stability of intestinal flora. Background Art

[0002] Constipation is a common clinical symptom caused by a variety of pathological factors, mainly manifested as infrequent bowel movements, difficult defecation or hard stools. Survey results show that a considerable proportion of people have experienced constipation. Since it rarely leads to hospitalization or death, its harm to health is often ignored. However, its high incidence rate and complex causes often bring a lot of pain and troubles to patients, seriously affecting people's physical health and quality of life. Modern medicine has confirmed that long-term constipation can induce various diseases, such as colorectal cancer, hypertension, cerebral apoplexy, and stroke. Therefore, when constipation symptoms occur, we need to pay attention to it and carry out appropriate treatment.

[0003] A large number of studies have shown that probiotics and dietary fiber are gradually gaining favor among researchers because of their safety and ability to effectively improve constipation.

[0004] Probiotics are active microorganisms that are beneficial to the human body. They can improve intestinal health through various mechanisms, especially in relieving constipation. Probiotics can help restore and maintain the balance of intestinal flora, inhibit the growth of harmful bacteria, promote the reproduction of beneficial bacteria, and thus improve the intestinal environment; at the same time, some probiotics can stimulate intestinal peristalsis and promote the movement and discharge of feces; in addition, probiotics can also increase the absorption of water in the intestine, promote the secretion of intestinal mucus, and make it easier to discharge; on the other hand, probiotics can enhance the immune function of the intestine and reduce intestinal inflammation. Intestinal inflammation and low immune function are also factors that lead to constipation.

[0005] Pitaya powder is a powder made from dried pitaya. It is rich in nutrients, including dietary fiber, vitamins, minerals and antioxidants. Dietary fiber can increase the volume and weight of stool, promote intestinal peristalsis, and help relieve constipation. Soluble fiber can absorb water and form a gel-like substance, making stool soft and easy to discharge. At the same time, fiber can serve as food for intestinal probiotics, promote the growth of probiotics, and improve the intestinal microecological environment, thereby helping to relieve constipation. In addition, rich antioxidants such as vitamin C and anthocyanins can help reduce intestinal inflammation and promote intestinal health. Among them, pitaya seeds are a kind of small black seeds in pitaya, which are usually eaten together with pitaya. It is also rich in dietary fiber, which can promote intestinal peristalsis, help digestion, and prevent constipation. Pitaya seed liquid is rich in various minerals, such as magnesium, calcium, iron and zinc. These minerals play an important role in immune function and overall metabolism.

[0006] A large number of studies have found that the intestinal microecological environment of constipation patients changes, mainly manifested in a significant decrease in the number of bifidobacteria that are beneficial to the human body, overgrowth of potential pathogenic bacteria and foreign pathogens, and constipation and other intestinal dysfunction symptoms. At present, Western medicine mostly uses laxatives, prokinetics and other drugs to treat it. Taking such drugs can only temporarily relieve the patient's symptoms, and has many side effects, can irritate the intestines, and easily form drug dependence. Therefore, it is very necessary to research and develop probiotic treatment methods.

[0007] There is an urgent need for a composition that can quickly promote defecation, improve intestinal motility, and balance intestinal flora, but at the same time has few side effects and does not cause intestinal flora disorders to meet people's needs. Summary of the invention

[0008] In one aspect, the present invention provides a probiotic composition comprising probiotics; wherein the number of viable probiotic bacteria in each 1 gram of the composition is 2.5-60 billion.

[0009] In some embodiments, bamboo extract is also included.

[0010] In some embodiments, the bamboo extract is 9-225 mg, preferably 12-75 mg, per 1 g of the composition.

[0011] In some embodiments, the bamboo extract is present in an amount of 15-25 mg per 1 g composition.

[0012] In some embodiments, the method for preparing the bamboo extract comprises washing and slicing fresh bamboo leaves for pretreatment; then steaming and filtering the treated bamboo to obtain a filtrate, which is then subjected to reduced pressure distillation to obtain the bamboo extract.

[0013] In some embodiments, probiotics include but are not limited to Bifidobacterium lactis, Lactobacillus rhamnosus, Bacillus coagulans, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium infantis, Lactobacillus casei, Bifidobacterium breve, Lactobacillus ramanensis, Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium thermophilus.

[0014] In some embodiments, the probiotic is Lactobacillus bifidum, Lactobacillus rhamnosus, or Lactobacillus plantarum.

[0015] In some embodiments, the number of viable probiotics in each 1 gram of the composition is 10-40 billion, preferably 17.5-20 billion.

[0016] In some embodiments, the composition further comprises water-soluble dietary fiber, including but not limited to polydextrose, fructooligosaccharides, isomaltooligosaccharides, lacto-oligosaccharides, xylo-oligosaccharides, soy oligosaccharides, agar powder, carboxymethyl cellulose, cereal dietary fiber, vegetable and fruit dietary fiber, nut and seed dietary fiber, and seaweed dietary fiber.

[0017] In some embodiments, cereal dietary fiber includes but is not limited to dietary fiber in whole grains, miscellaneous grains, beans and the like, specifically including sweet potatoes, purple sweet potatoes, corn, brown rice, barley, buckwheat, oats, kidney beans, mung beans, adzuki beans, broad beans, peas, cowpeas, lentils, and black beans.

[0018] In some embodiments, vegetable and fruit dietary fibers include, but are not limited to, broccoli, carrots, apples, bananas, cucumbers, loofahs, eggplants, and tomatoes.

[0019] In some embodiments, nut and seed dietary fibers include, but are not limited to, almonds, flax seeds, chia seeds, walnuts, pistachios, almonds, pine nuts.

[0020] In some embodiments, seaweed dietary fiber includes but is not limited to wakame, nori, and kelp.

[0021] In some embodiments, the ratio of the number of viable probiotics to the mass of water-soluble dietary fiber is 25-60 billion:36-810 mg, preferably 10-40 billion:48-270 mg, and further preferably 175-20 billion:60-90 mg.

[0022] In some embodiments, the mass of water-soluble dietary fiber in each 1 gram of the composition is 36-810 mg, preferably 48-270 mg, and more preferably 60-90 mg.

[0023] In some embodiments, the composition further comprises sugar alcohols, including but not limited to sorbitol, maltitol, mannitol, lactitol, erythritol, and xylitol.

[0024] In some embodiments, the sugar alcohol is sorbitol.

[0025] In some embodiments, the ratio of the number of viable probiotics to the mass of sugar alcohol is 2.5-60 billion: 0.36-8.1 g, preferably 10-40 billion: 0.48-2.7 g, and further preferably 17.5-20 billion: 0.6-0.9 g.

[0026] In some embodiments, the mass of the sugar alcohol in each 1 g of the composition is 0.36-8.1 g, preferably 0.48-2.7 g, and more preferably 0.6-0.9 g.

[0027] In some embodiments, pitaya powder is also included, and the mass ratio of the number of live probiotics to pitaya powder is 100-240 billion: 0.5-10 mg, preferably 400-160 billion: 0.5-10 mg, and further preferably 700-80 billion: 0.5-10 mg.

[0028] In some embodiments, the mass of pitaya powder in each 1g of the composition is 0.1-2.5mg, specifically 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5.

[0029] In some embodiments, yogurt powder is also included.

[0030] In another aspect, the present invention provides a product, which is prepared from the above composition into tablets, capsules, powders, oral liquids, suspensions, jellies, soft candies or crystal balls.

[0031] In some embodiments, the composition described above is prepared into a powder.

[0032] In some embodiments, the powder preparation method is to mix the powders of various substances in the composition evenly, and then fill them to obtain the product.

[0033] On the other hand, the present invention provides a use of the above-mentioned composition and product in rapidly promoting defecation, maintaining the balance of intestinal flora, and improving intestinal motility.

[0034] In some embodiments, the composition provided by the present invention is taken in an amount of 1-2 bags per day, which can be drunk directly or taken with warm water below 37 degrees.

[0035] Beneficial effects of the present invention:

[0036] 1. Under the action of probiotics, water-soluble dietary fiber and pitaya powder, the composition can quickly promote defecation in adults within 24 hours and quickly solve the constipation problem of patients.

[0037] 2. The composition is highly safe, contains no laxative ingredients, and avoids irritating the intestines.

[0038] 3. The three probiotics can balance the intestinal flora, enabling patients to maintain long-term regular bowel movements.

[0039] 4. Adding bamboo extract powder during the preparation of the powder can improve the fluidity of the powder and make the components mixed more evenly. DETAILED DESCRIPTION

[0040] In order to facilitate the understanding of those skilled in the art, the present invention is further described below in conjunction with embodiments, and the contents mentioned in the implementation modes are not intended to limit the present invention.

[0041] Example 1

[0042] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium subspecies HN019, Lactobacillus rhamnosus GG, Lactobacillus plantarum PL15A, and the number of live probiotic bacteria is 75 billion; polydextrose 300mg; sorbitol 3000mg; appropriate amount of pitaya powder, and bamboo extract powder 80mg.

[0043] Example 2

[0044] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium lactis subspecies HN019, rhamnosus lactobacillus GG, and plantarum lactobacillus PL15A, with a live bacterial count of 70 billion; polydextrose 240mg; sorbitol 3600mg; an appropriate amount of pitaya powder, and bamboo extract powder 60mg.

[0045] Example 3

[0046] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium subspecies HN019, Lactobacillus rhamnosus GG, Lactobacillus plantarum PL15A, and the number of live probiotic bacteria is 80 billion; polydextrose 360mg; sorbitol 2400mg; appropriate amount of pitaya powder, and bamboo extract powder 100mg.

[0047] Comparative Example 1

[0048] A composition of 4g, wherein the probiotic powder contains Bifidobacterium bifidum B11, Lactobacillus rhamnosus GG, Lactobacillus plantarum PL15A, and the number of live probiotics is 75 billion; polydextrose 300mg; sorbitol 3000mg; appropriate amount of pitaya powder, and bamboo extract powder 80mg.

[0049] Comparative Example 2

[0050] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium lactis subspecies HN019, Lactobacillus rhamnosus GG, Lactobacillus casei LC-12, and the number of live probiotic bacteria is 75 billion; polydextrose 300mg; sorbitol 3000mg; appropriate amount of pitaya powder, and bamboo extract powder 80mg.

[0051] Comparative Example 3

[0052] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium lactis subspecies HN019, Lactobacillus casei LC-12, Lactobacillus plantarum PL15A, the number of live probiotic bacteria is 75 billion; polydextrose 300mg; sorbitol 3000mg, and bamboo extract powder 80mg.

[0053] Comparative Example 4

[0054] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium, Lactobacillus rhamnosus and Lactobacillus plantarum, and the number of live probiotic bacteria is 75 billion; polydextrose 300mg; lactitol 3000mg; appropriate amount of pitaya powder and 80mg of bamboo extract powder.

[0055] Comparative Example 5

[0056] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium, Lactobacillus rhamnosus and Lactobacillus plantarum, and the number of live probiotic bacteria is 75 billion; oligofructose 300mg; sorbitol 3000mg; appropriate amount of pitaya powder and bamboo extract powder 80mg.

[0057] Comparative Example 6

[0058] A composition of 4g, wherein the probiotic powder contains animal bifidobacterium subspecies HN019, Lactobacillus rhamnosus GG, Lactobacillus plantarum PL15A, the number of live probiotic bacteria is 75 billion; polydextrose 300mg; sorbitol 3000mg; and an appropriate amount of pitaya fruit powder.

[0059] Example 6 Evaluation test of defecation promotion efficacy

[0060] 55 mice were randomly divided into a blank control group, a model control group, Example 1-8 groups and a positive control group, with 5 mice in each group. Except for the blank control group, mice in other groups were deprived of water but not food for 72 hours to create a dryness and dehydration constipation model.

[0061] The drugs were administered in the following manner: blank control group: free diet; model control group: deprivation of water but not food; positive control group: deprivation of water but not food, and fed with compound diphenoxylate at a dose of 15 mg / (kg·d); Example 1-8 group: at a dose of 250 mg / (kg·d), the composition of Example 1-8 of the present invention was mixed with distilled water to form a suspension with a concentration of 2 g / L, and then gavage was performed.

[0062] After administration, the mice were placed in a mouse cage with a clean white filter paper underneath. The first defecation time (min) of each mouse and the total weight of mouse feces within 24 hours were observed and recorded. The results are shown in Table 1.

[0063] Time to first defecation: the time from the end of gavage to the first defecation.

[0064] Table 1

[0065]

[0066] Example 7 Effect of the composition of the example on improving constipation

[0067] Purpose of tasting: To verify the effect of Example 1 in improving constipation

[0068] Population inclusion: The inclusion criteria for subjects are as follows (subjects are included as long as they meet one of the criteria):

[0069] ① Bowel movement frequency: <2 times / week;

[0070] ②Those who have difficulty defecating;

[0071] ③The stool is hard;

[0072] ④Those who think they need to improve their bowel movements or are constipated

[0073] Experimental design: 17 people in total took Example 1 for 3 consecutive days;

[0074] Dosage: For those who meet 1-2 of the inclusion criteria, take 1 bag on the first day, and increase to 2 bags as appropriate based on the effect on the first day;

[0075] Those who meet 3 of the inclusion criteria can take 2 bags directly on the first day;

[0076] For those who only meet inclusion criteria ④, take 1 bag on the first day, and increase to 2 bags according to the effect on the first day;

[0077] Questionnaire content: 1. Main criteria: ① Defecation frequency; ② Defecation condition; ③ Stool condition; ④ Stool volume; ⑤ Defecation time;

[0078] 2. Secondary criteria: record the presence or absence of adverse reactions (nausea, vomiting, diarrhea, abdominal pain, abnormal stool, etc.).

[0079] Table 2

[0080]

[0081]

[0082] Example 8 Determination of powder fluidity

[0083] The repose angles of the compositions prepared in Examples 1-3 and Comparative Example 6 were measured by the injection method. Example 1 had the best fluidity, and Examples 2-3 had angles between 30 and 40, with little difference; while the repose angle of Comparative Example 6 was greater than 50%, greater than 45 degrees, and had poor fluidity.

[0084] Table 3

[0085] sample Angle of repose / ° Example 1 32.24 Example 2 35.52 Example 3 35.91 Comparative Example 6 55.68

[0086] It should be understood that the detailed description of the technical solutions of the present invention by means of the preferred embodiments is illustrative rather than restrictive. A person skilled in the art may modify the technical solutions described in the embodiments, or replace some of the technical features by equivalents, based on reading the specification of the present invention; and these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A pitaya powder probiotic composition, characterized in that: Contains probiotics; wherein the number of live probiotics in the composition is 2.5-60 billion / g.

2. The composition according to claim 1, characterized in that Also contains bamboo extract.

3. The composition according to claim 2, characterized in that The bamboo extract is present in the composition at 9-225 mg / g, preferably 12-75 mg / g.

4. The composition according to claim 3, characterized in that The bamboo extract is present in the composition at 15-25 mg / g.

5. The composition according to claim 2, characterized in that The preparation method comprises the following steps: washing and slicing fresh bamboo leaves for pretreatment; then steaming and filtering the processed bamboo leaves to obtain a filtrate, and then performing reduced pressure distillation to obtain a bamboo extract.

6. The composition according to claim 1, characterized in that The probiotics are one or a combination of Bifidobacterium lactis, Lactobacillus rhamnosus, Bacillus coagulans, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium infantis, Lactobacillus casei, Bifidobacterium breve, Lactobacillus ramanensis, Bifidobacterium longum, Bifidobacterium breve, and Bifidobacterium thermophilus.

7. The composition according to claim 6, characterized in that The probiotics are bifidobacterium animalis subspecies, Lactobacillus rhamnosus, and Lactobacillus plantarum.

8. The composition according to claim 1, characterized in that The number of viable probiotics in the composition is 10-40 billion / g, preferably 17.5-20 billion / g.

9. The composition according to claim 1, characterized in that The composition also contains water-soluble dietary fiber, preferably one or a combination of polydextrose, fructooligosaccharide, isomaltooligosaccharide, lacto-oligosaccharide, xylo-oligosaccharide, soybean oligosaccharide, agar powder, carboxymethyl cellulose, cereal dietary fiber, vegetable and fruit dietary fiber, nut and seed dietary fiber, and seaweed dietary fiber.

10. The composition according to claim 9, characterized in that The ratio of the number of viable probiotics to the mass of water-soluble dietary fiber is 25-60 billion:36-810 mg, preferably 10-40 billion:48-270 mg, and more preferably 175-20 billion:60-90 mg.

11. The composition according to claim 1, characterized in that The composition further comprises sugar alcohol, preferably one or a combination of sorbitol, maltitol, mannitol, lactitol, erythritol and xylitol.

12. The composition according to claim 11, characterized in that The sugar alcohol is sorbitol.

13. The composition according to claim 11, characterized in that The ratio of the number of viable probiotics to the mass of sugar alcohol is 2.5-60 billion: 0.36-8.1 g, preferably 10-40 billion: 0.48-2.7 g, and more preferably 17.5-20 billion: 0.6-0.9 g.

14. The composition according to claim 1, characterized in that Also contains dragon fruit powder.

15. The composition according to claim 14, characterized in that The mass ratio of the number of live probiotics to pitaya powder is 10-240 billion: 0.5-10 mg, preferably 40-160 billion: 0.5-10 mg, and further preferably 70-80 billion: 0.5-10 mg.

16. The composition according to claim 1, characterized in that Also contains yogurt powder.

17. A product, characterized in that The composition according to any one of claims 1 to 16 is prepared into tablets, capsules, powders, oral liquids, suspensions, jellies, soft candies or crystal balls.

18. The product according to claim 17, characterized in that A powder prepared from the composition according to any one of claims 1 to 16.

19. Use of the composition according to any one of claims 1 to 16 and the product according to any one of claims 17 to 18 in rapidly promoting defecation, maintaining the balance of intestinal flora, and improving intestinal motility.

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