Antibacterial and antifungal lotion and its application

Abstract

The application discloses an antibacterial and itch-relieving lotion and application thereof, and belongs to the technical field of biology. The antibacterial and itch-relieving lotion contains an antibacterial and itch-relieving composition, and the composition comprises the following components: nano gold, celery extract and multi-strain co-cultured extract. The strains used in the multi-strain co-cultured extract are Lactobacillus gasseri, Bifidobacterium and yeast. The antibacterial and itch-relieving lotion has good antibacterial effect and is mild and non-irritating due to the synergistic effect among the components of the nano gold, the celery extract and the multi-strain co-cultured extract.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a bacteriostatic and anti-itching lotion and application thereof. BACKGROUND

[0002] Gynecological inflammation is often caused by bacteria or fungi such as Candida albicans, Staphylococcus aureus, Escherichia coli, Neisseria gonorrhoeae, Proteus mirabilis, trichomonas and Streptococcus agalactiae, etc. These pathogens not only harm women's health, but also seriously affect their daily life. However, many traditional female private care products contain strong bactericidal ingredients such as chlorhexidine gluconate, benzalkonium chloride and sophoramine, which can destroy the normal flora balance of the private parts, and long-term use can cause discomfort such as itching and dryness of the private parts. Therefore, there is an urgent need in the market for a lotion product that can effectively inhibit the growth of pathogens and gently care for the health of the private parts without causing irritation. SUMMARY

[0003] The purpose of the present application is to provide a bacteriostatic and anti-itching lotion and application thereof, which uses nanogold, celery extract and multi-strain co-culture extract as active substances, is safe, has strong bacteriostatic effect and is mild and non-irritating.

[0004] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme:

[0005] In a first aspect, the present application provides a bacteriostatic and anti-itching composition comprising the following components: nanogold, celery extract and multi-strain co-culture extract.

[0006] Preferably, the bacteriostatic and anti-itching composition comprises the following components by mass:

[0007] 0.005-0.01 parts of nanogold;

[0008] 5-10 parts of celery extract;

[0009] 5-10 parts of multi-strain co-culture extract;

[0010] The particle size of the nanogold is 20-200 nm;

[0011] The preparation method of the multi-strain co-culture extract comprises the following steps:

[0012] S1. Prepare MRS medium;

[0013] S2. Lactobacillus gasseri, Bifidobacterium and yeast were inoculated in culture vessels containing MRS liquid medium at 2*10 6 CFU / mL respectively for co-culture, and the culture conditions were as follows: temperature 25-35℃, pH 5.0-5.5, and culture time 8-12h;

[0014] S3. Sterile air is introduced into the culture vessel after the end of the culture, and the culture vessel is supplemented with MRS medium to half the volume of the liquid medium, and then the culture is continued for 4-6 h;

[0015] S4. After the end of the culture, the culture is subjected to ultrasonic disruption, high-temperature instantaneous sterilization, filtration, and the filtrate is collected and freeze-dried to obtain a multi-strain co-culture extract;

[0016] wherein,

[0017] The preservation number of the Lactobacillus gasseri is: CGMCC No. 6361;

[0018] The preservation number of the Bifidobacterium is: ATCC 15696;

[0019] The preservation number of the yeast is: GIMCC 2.85.

[0020] Further, the bacteriostatic anti-itch composition is composed of the following components by mass fraction:

[0021] Nano gold 0.005-0.007 parts;

[0022] Celery extract 5-7 parts;

[0023] Multi-strain co-culture extract 5-7 parts;

[0024] The particle size of the nano gold is 20-200 nm.

[0025] Most preferably, the bacteriostatic anti-itch composition is composed of the following components by mass fraction:

[0026] Nano gold 0.007 parts;

[0027] Celery extract 7 parts;

[0028] Multi-strain co-culture extract 7 parts;

[0029] The particle size of the nano gold is 20-200 nm.

[0030] In a second aspect, the present application provides a bacteriostatic and anti-itch lotion, which comprises the following ingredients: 7-9 wt% bacteriostatic anti-itch composition, 0.5-5 wt% water-soluble vitamin E, 0.1-1.5 wt% ascorbic acid tetraisopalmitate, 20-25 wt% glycerol, and the balance of sterile water.

[0031] In a third aspect, the present application provides a preparation method of a bacteriostatic and anti-itch lotion, and the specific steps are as follows:

[0032] S1. Dissolve the celery extract, multi-strain co-culture extract, and water-soluble vitamin E in sterile water and heat to 40-45℃, and stir uniformly to obtain phase A;

[0033] S2. Add nano-gold into sterile water, and ultrasonically shake until transparent to obtain phase B;

[0034] S3. Mix glycerol and ascorbic acid tetraisopalmitate uniformly to obtain phase C;

[0035] S4. Mix phases A, B and C uniformly, filter, sterilize, and fill to obtain the bacteriostatic and anti-itching lotion.

[0036] Compared with the prior art, the bacteriostatic and anti-itching composition is prepared by mixing nano-gold, celery extract and multi-strain co-cultured extract in a specific mass ratio; the bacteriostatic and anti-itching composition has good bacteriostatic effect through the synergistic effect of the components. Further, the bacteriostatic and anti-itching lotion is prepared by adding glycerol, ascorbic acid tetraisopalmitate, water-soluble vitamin E and sterile water to the composition. The bacteriostatic and anti-itching lotion can effectively inhibit Staphylococcus aureus, Escherichia coli and Candida albicans, and has a certain inhibitory effect on Neisseria, Streptococcus agalactiae, Proteus mirabilis and trichomonas, and can be used for preventing and treating diseases caused by infection of the above bacteria. The bacteriostatic and anti-itching lotion is mild and non-irritating, and has excellent production and application value. DETAILED DESCRIPTION

[0037] In order to better understand the present application, the present application will be further described below in conjunction with specific examples, wherein the terms used in the examples are used for describing specific specific embodiments, and do not constitute a limitation on the scope of protection of the present application.

[0038] The test methods in the following tests are generally carried out under conventional conditions or under the conditions recommended by the manufacturer. Unless otherwise specified, the percentages and parts are calculated by weight.

[0039] Some raw materials and sources are as follows:

[0040] Nano-gold (20-200 nm, 500-1000 nm): purchased from Wuhan Kemik Biomedicine Technology Co., Ltd.

[0041] Celery extract: purchased from Xi'an Xinheng Biotechnology Co., Ltd.

[0042] The preservation number of Lactobacillus gasseri is CGMCC No.6361.

[0043] The preservation number of Bifidobacterium is ATCC 15696.

[0044] The preservation number of yeast is GIMCC 2.85.

[0045] MRS medium: purchased from Thermo Fisher Scientific, item number: CM1153R.

[0046] Staphylococcus aureus: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., item number: B81854(A).

[0047] Escherichia coli: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., item number B81038.

[0048] Candida albicans: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., item number BMZ132601.

[0049] Neisseria: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., item number BMZ133675.

[0050] Streptococcus agalactiae: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., item number B80383.

[0051] Proteus mirabilis: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., item number BMZ122593.

[0052] Trichomonas: purchased from Xinyang Laiyao Biotechnology Co., Ltd., item number ATCC 50143.

[0053] The preparation method of the multi-strain co-culture extract of the application is as follows:

[0054] Multi-strain co-culture extract 1:

[0055] S1: Prepare MRS medium, refer to the preparation instructions of MRS medium;

[0056] S2: Inoculate Lactobacillus gasseri, Bifidobacterium, and yeast with 2*10 6 CFU / mL into a 20L fermenter containing 8L of MRS liquid medium for co-culture, and the culture conditions are: temperature 30℃, pH 5.3, and culture for 10h;

[0057] S3: After the culture is completed, sterile air is introduced into the fermenter, 4L of MRS medium is supplemented, and the culture is continued for 5h; wherein the sterile air flow rate is 1L / min;

[0058] S4: After the culture is completed, the culture is subjected to ultrasonic crushing, high-temperature instantaneous sterilization, and filtration, the filtrate is collected and freeze-dried to obtain a multi-strain co-culture extract, wherein the ultrasonic crushing conditions are: frequency: 20kHz, time: 20min, power: 650w, and temperature: 28℃; the high-temperature instantaneous sterilization conditions are: temperature 135℃ and time 5s; and the filtration conditions are: using a 0.45μm microporous filter membrane for filtration.

[0059] Multi-strain co-culture extract 2:

[0060] S1: Prepare MRS culture medium, refer to the preparation method of MRS culture medium;

[0061] S2: Lactobacillus gasseri, Bifidobacterium and Saccharomyces cerevisiae were inoculated into a 20L fermenter containing 8L MRS liquid medium at 2*10 6 CFU / mL respectively for co-culture, and the culture conditions were as follows: temperature 25℃, pH 5.0, and culture time 12h;

[0062] S3: After the culture was completed, sterile air was introduced into the fermenter, 4L of MRS culture medium was added, and the culture was continued for 4h; the flow rate of sterile air was 1L / min.

[0063] S4: After the culture was completed, the culture was subjected to ultrasonic crushing, high-temperature instantaneous sterilization, and filtration, and the filtrate was collected and freeze-dried to obtain a multi-strain co-culture extract, wherein the ultrasonic crushing conditions were as follows: frequency 20kHz, time 20min, power 650w, and temperature 28℃; the high-temperature instantaneous sterilization conditions were as follows: temperature 135℃ and time 5s; and the filtration conditions were as follows: 0.45μm microporous filter membrane was used for filtration.

[0064] S1: Prepare MRS culture medium, refer to the preparation method of MRS culture medium;

[0065] S2: Lactobacillus gasseri, Bifidobacterium and Saccharomyces cerevisiae were inoculated into a 20L fermenter containing 8L MRS liquid medium at 2*10 6 CFU / mL respectively for co-culture, and the culture conditions were as follows: temperature 35℃, pH 5.5, and culture time 8h;

[0066] S3: After the culture was completed, sterile air was introduced into the fermenter, 4L of MRS culture medium was added, and the culture was continued for 6h; the flow rate of sterile air was 1L / min.

[0067] S4: After the culture was completed, the culture was subjected to ultrasonic crushing, high-temperature instantaneous sterilization, and filtration, and the filtrate was collected and freeze-dried to obtain a multi-strain co-culture extract, wherein the ultrasonic crushing conditions were as follows: frequency 20kHz, time 20min, power 650w, and temperature 28℃; the high-temperature instantaneous sterilization conditions were as follows: temperature 135℃ and time 5s; and the filtration conditions were as follows: 0.45μm microporous filter membrane was used for filtration.

[0068] Multi-strain co-culture extract ①:

[0069] Different from the multi-strain co-culture extract 1, only 3*10 6 CFU / mL of Lactobacillus gasseri and 3*10 6CFU / mL Lactobacillus gasseri (i.e. the total number of viable bacteria added to the medium is the same as in Multispecies co-culture extract 1), the remaining steps are the same as in Multispecies co-culture extract 1.

[0070] Multispecies co-culture extract 2:

[0071] Different from Multispecies co-culture extract 1, only 3*10 6 CFU / mL Bifidobacterium and 3*10 6 CFU / mL Lactobacillus gasseri (i.e. the total number of viable bacteria added to the medium is the same as in Multispecies co-culture extract 1), the remaining steps are the same as in Multispecies co-culture extract 1.

[0072] Multispecies co-culture extract 3:

[0073] Different from Multispecies co-culture extract 1, only 3*10 6 CFU / mL Bifidobacterium and 3*10 6 CFU / mL Lactobacillus gasseri (i.e. the total number of viable bacteria added to the medium is the same as in Multispecies co-culture extract 1), the remaining steps are the same as in Multispecies co-culture extract 1.

[0074] Multispecies co-culture extract 4:

[0075] Different from Multispecies co-culture extract 1, only 6*10 6 CFU / mL Bifidobacterium (i.e. the total number of viable bacteria added to the medium is the same as in Multispecies co-culture extract 1), the remaining steps are the same as in Multispecies co-culture extract 1.

[0076] Multispecies co-culture extract 5:

[0077] Different from Multispecies co-culture extract 1, only 6*10 6 CFU / mL Lactobacillus gasseri (i.e. the total number of viable bacteria added to the medium is the same as in Multispecies co-culture extract 1), the remaining steps are the same as in Multispecies co-culture extract 1.

[0078] Multispecies co-culture extract 6:

[0079] Different from Multispecies co-culture extract 1, only 6*10 6 CFU / mL Lactobacillus gasseri (i.e. the total number of viable bacteria added to the medium is the same as in Multispecies co-culture extract 1), the remaining steps are the same as in Multispecies co-culture extract 1.

[0080] The mass fraction of each component in the bacteriostatic and anti-itch composition of the examples and comparative examples of the present application is shown in Table 1 below:

[0081] Table 1 Mass fraction of each component in the bacteriostatic and anti-itch composition

[0082]

[0083]

[0084] Note: "-" represents no addition, the missing mass parts are replaced with sterile water, composition The nano gold particle size used in the composition is 500nm-1000nm.

[0085] A bacteriostatic and scab washing solution, the mass percentage of its components is shown in the following table 2.

[0086] Table 2 Mass percentage of each component of the bacteriostatic and scab washing solution

[0087]

[0088]

[0089]

[0090] The above bacteriostatic and scab washing solutions 1, 4, 5, 6, 7 and the bacteriostatic and scab washing solution The preparation method is as follows:

[0091] S1. Dissolve the celery extract, multi-strain co-culture extract and water-soluble vitamin E in sterile water and heat to 43℃, stir uniformly to obtain phase A;

[0092] S2. Add nano gold to sterile water, ultrasonic oscillation until transparent to obtain phase B;

[0093] S3. Mix glycerol and ascorbic acid tetraisopalmityl uniformly to obtain phase C;

[0094] S4. Mix phase A, phase B and phase C uniformly, filter, sterilize, fill, to obtain the bacteriostatic and scab washing solution.

[0095] The preparation method of the bacteriostatic and scab washing solution 2 is as follows:

[0096] S1. Dissolve the celery extract, multi-strain co-culture extract and water-soluble vitamin E in sterile water and heat to 40℃, stir uniformly to obtain phase A;

[0097] S2. Add nano gold to sterile water, ultrasonic oscillation until transparent to obtain phase B;

[0098] S3. Mix glycerol and ascorbic acid tetraisopalmityl uniformly to obtain phase C;

[0099] S4. Mix phase A, phase B and phase C uniformly, filter, sterilize, fill, to obtain the bacteriostatic and scab washing solution.

[0100] The preparation method of the bacteriostatic and pruritus-relieving lotion 3 is as follows:

[0101] S1. The celery extract, the multi-strain co-cultured extract, and the water-soluble vitamin E are dissolved in sterile water and heated to 45℃, and stirred uniformly to obtain phase A;

[0102] S2. The nano-gold is added to sterile water, and ultrasonic oscillation is performed until it is transparent to obtain phase B;

[0103] S3. The glycerol and ascorbic acid tetraisopalmitate are uniformly mixed to obtain phase C;

[0104] S4. Phase A, phase B, and phase C are uniformly mixed, filtered, sterilized, filled, and sealed to obtain the bacteriostatic and pruritus-relieving lotion.

[0105] The preparation method of the bacteriostatic and pruritus-relieving lotion blank control is as follows:

[0106] First, the water-soluble vitamin E is dissolved in sterile water and heated to 43℃, and stirred uniformly to obtain phase A; then, the glycerol and ascorbic acid tetraisopalmitate are uniformly mixed to obtain phase B; finally, phase A and phase B are uniformly mixed, filtered, sterilized, and filled to obtain the bacteriostatic and pruritus-relieving lotion.

[0107] The bacteriostatic and pruritus-relieving lotion

[0108] The bacteriostatic and pruritus-relieving lotion The used components are completely same as those of the bacteriostatic and pruritus-relieving lotion 1, and the preparation method is as follows:

[0109] The celery extract, the multi-strain co-cultured extract, the water-soluble vitamin E, the nano-gold, the glycerol, the ascorbic acid tetraisopalmitate, and the sterile water are uniformly mixed, heated to 43℃, filtered, sterilized, and filled to obtain the bacteriostatic and pruritus-relieving lotion.

[0110] Example 1: Bacteriostatic test

[0111] Test sample: bacteriostatic and pruritus-relieving lotions 1-7 and bacteriostatic and pruritus-relieving lotion and bacteriostatic and pruritus-relieving lotion blank control.

[0112] According to the microbial killing experiment method in the Technical Standard for Disinfection (2002 edition), the killing rates of the test samples on Candida albicans, Escherichia coli, Staphylococcus aureus, Neisseria, Streptococcus agalactiae, Proteus mirabilis, and trichomonas were determined, and the results are shown in Table 1.

[0113] Table 1: Bacteriostatic test results

[0114]

[0115]

[0116] Table 1 shows that Antibacterial and Antipruritic Lotions 1-7 have good inhibitory effects on Escherichia coli, Staphylococcus aureus, and Candida albicans, and also have certain inhibitory effects on Neisseria gonorrhoeae, Streptococcus agalactiae, Proteus mirabilis, and Trichomonas vaginalis. Comparison of the antibacterial effects of Antibacterial and Antipruritic Lotion 1 with Antibacterial and Antipruritic Lotions ①-③ and ④-⑥ indicates that there is a certain synergistic effect among different bacterial species in the multi-species co-culture extract. The comparison of Antibacterial and Antipruritic Lotion 1 with Antibacterial and Antipruritic Lotions... The results showed that there was a certain synergistic effect between nano-gold, celery extract, and multi-strain co-culture extract; a comparison of antibacterial and antipruritic lotion 1 and antibacterial and antipruritic lotion... The results show that the particle size of the gold nanoparticles has a certain impact on the antibacterial and antipruritic lotion; comparing antibacterial and antipruritic lotion 1 with antibacterial and antipruritic lotion 2... The results show that the preparation method provided by the present invention can further improve the antibacterial effect of the antibacterial and antipruritic wash.

[0117] Example 2: Vaginal mucosal stimulation test

[0118] Test substances: Antibacterial and antipruritic lotion 1-7, Antibacterial and antipruritic lotion And the blank of the antibacterial and antipruritic wash.

[0119] Experimental methods: Referring to the vaginal mucosal irritation test method in the "Disinfection Technical Specifications" (2002 edition), the irritation of the test substance to the vaginal mucosa was tested.

[0120] Table 2. Grading Standards for Adverse Skin Reactions

[0121]

[0122]

[0123] Based on the above experimental results, the antibacterial and antipruritic lotion 1-7 and the antibacterial and antipruritic lotion prepared in this invention The antibacterial and antipruritic wash solution is non-irritating to the vaginal mucosa.

[0124] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. A bacteriostatic anti-itch lotion characterized in that, The anti-itch lotion is composed of the following components by mass percentage: Bacteriostatic anti-itch composition 7-9wt%; Water-soluble vitamin E 0.5-5wt%; Ascorbyl tetraisopalmitate 0.1-1.5wt%; Glycerol 20-25wt%; Sterile water balance; The bacteriostatic anti-itch composition is composed of the following mass parts of raw materials: Nano gold 0.005-0.01 parts; Celery extract 5-10 parts; Multi-strain co-culture extract 5-10 parts; The nano gold is nano gold powder with a particle size of 20-200 nm; The preparation method of the multi-strain co-culture extract comprises the following steps: S1. Configure MRS medium; S2. Lactobacillus gasseri, Bifidobacterium, and Saccharomyces were inoculated at 2*10 6 CFU / mL into culture vessels containing MRS liquid medium for co-culturing, and the culture conditions were 25-35°C, pH 5.0-5.5, and 8-12 h of culture. S3. After the culture is completed, sterile air is introduced into the culture container, and the culture container is supplemented with half the volume of MRS medium, and then cultured for 4-6h; S4. After the culture is completed, the culture is subjected to ultrasonic crushing, high-temperature instantaneous sterilization, and filtration, and the filtrate is collected and freeze-dried to obtain a multi-strain co-culture extract; Among them, The preservation number of the Lactobacillus gasseri is: CGMCC No.6361; The preservation number of the Bifidobacterium is: ATCC 15696; The preservation number of the yeast is: GIMCC 2.85; The preparation method of the bacteriostatic anti-itch lotion comprises the following steps: S1. Dissolve the celery extract, multi-strain co-culture extract, and water-soluble vitamin E in sterile water and heat to 40-45℃, stir uniformly, and obtain phase A; S3. Add nano gold to sterile water, ultrasonically shake until transparent, and obtain phase B; S3. Mix glycerol and ascorbyl tetraisopalmitate uniformly to obtain phase C; S4. Mix phases A, B, and C uniformly, filter, sterilize, and fill.

2. The bacteriostatic anti-itch lotion of claim 1, wherein The bacteriostatic anti-itch composition is composed of the following mass parts of components: Nano gold 0.005-0.007 parts; Celery extract 5-7 parts; Multi-strain co-culture extract 5-7 parts.

3. The bacteriostatic anti-itch lotion of claim 1, wherein The bacteriostatic anti-itch composition is composed of the following mass parts of components: Nano gold 0.007 parts; Celery extract 7 parts; Multi-strain co-culture extract 7 parts.

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