Oxidized resveratrol-containing multi-dimensional anti-pollution composition suitable for sensitive skin and application of oxidized resveratrol-containing multi-dimensional anti-pollution composition

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses an oxyresveratrol-containing multi-vitamin anti-pollution composition suitable for sensitive skin and application of the oxyresveratrol-containing multi-vitamin anti-pollution composition, the multi-vitamin anti-pollution composition comprises oxyresveratrol, epigallocatechin gallic acid glucoside, acetylchitosamine and annona squamosa extract; the mass ratio of the oxyresveratrol to the epigallocatechin gallic acid glucoside to the acetylchitosamine to the annona squamosa extract is (0.1 to 3) to (0.001 to 0.1) to (1 to 10) to (0.1 to 5). The anti-pollution composition provided by the invention achieves the effects of relieving, repairing and whitening by simultaneously resisting various pollutants such as air pollution, ultraviolet rays, heavy metals and the like, and comprehensively solves the problems of yellow smell and sensitivity of skin.

Description

Technical Field

[0001] The present application relates to the field of cosmetic technology, and in particular to a multidimensional anti-pollution composition containing oxidized resveratrol suitable for sensitive skin and its application. Background Art

[0002] The impact of environmental pollution on human skin has received increasing attention. For example, air pollution, ultraviolet radiation, and chemicals may have a negative impact on skin health. Fine particles in the air (such as PM2.5) can penetrate the skin barrier and enter the deep layers of the skin, causing inflammation, allergies, and accelerated skin aging. Ozone in the air can oxidize skin lipids, causing dullness, dryness, and decreased elasticity of the skin. It can also irritate the skin, causing redness, swelling, and sensitivity. UVA radiation can penetrate the dermis, destroy collagen and elastic fibers, causing skin sagging and wrinkles; UVB mainly affects the epidermis, which may lead to sunburn, pigmentation, and increased risk of skin cancer. Heavy metal pollutants such as lead and mercury can be absorbed through the skin, affecting skin health and even causing poisoning reactions. Certain chemicals (such as certain ingredients in cosmetics) can damage the skin barrier, causing dryness, allergies, and irritation.

[0003] Currently, many skin care products can only focus on a single function, such as anti-oxidation, moisturizing or anti-inflammatory, and lack comprehensive anti-pollution, yellowing removal, and sensitivity improvement solutions. Few products can effectively remove yellowing and repair the skin at the same time. Existing research often focuses on whitening and ignores the root cause of the yellowing problem. In addition, the anti-pollution ingredients in existing research can form a temporary protective layer on the surface of the skin, but cannot effectively isolate pollutants or repair skin damage for a long time. Summary of the invention

[0004] The purpose of the present application is to overcome the deficiencies of the above-mentioned prior art and to provide a multi-dimensional anti-pollution composition containing oxidized resveratrol suitable for sensitive skin and its application. The anti-pollution composition provided in the present application can achieve the effects of soothing, repairing and whitening by simultaneously resisting multiple pollutants such as air pollution, ultraviolet rays, heavy metals, etc., and comprehensively solve the problems of yellow skin and sensitivity.

[0005] To achieve the above purpose, the technical solution adopted by this application is:

[0006] The present application provides a multidimensional anti-pollution composition, which comprises oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract;

[0007] The mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.1-3): (0.001-0.1): (1-10): (0.1-5).

[0008] The inventors of the present application have found that the use of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract in a specific ratio can simultaneously resist multiple pollutants such as air pollution, ultraviolet rays, heavy metals, etc., and achieve soothing, repairing and whitening effects. Among them, the combination of oxidized resveratrol and epigallocatechin gallate glucoside can not only neutralize the free radicals caused by pollution through antioxidant effects, reduce the oxidative damage of environmental pollution to the skin, and protect the skin from UV radiation damage, but also can jointly resist air pollution by strengthening the skin barrier, effectively reduce the oxidative stress of the skin, prevent damage caused by ultraviolet rays, and relieve skin inflammation, improve skin sensitivity and redness.

[0009] At the same time, the addition of acetyl glucosamine can promote skin moisturizing and repair damaged skin barrier. It helps reduce skin inflammation, improve skin smoothness and elasticity, and promote uniform pigmentation, reduce skin yellowness, and make it more able to cope with environmental stress.

[0010] Adding the cherimoya extract to the multidimensional anti-pollution composition of the present application can protect the skin from environmental pollution and ultraviolet damage, can significantly reduce the redness and sensitivity of the skin caused by pollution, and has antibacterial properties, which helps to keep the skin clean and healthy. The combined effect of the above substances can make the skin more comfortable and healthy when facing environmental pollution.

[0011] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.2-2): (0.005-0.05): (4-8): (0.5-3).

[0012] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.5-1): (0.01-0.02): (6-7): (1-2).

[0013] The present application has found that when oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract are used in the above-mentioned mass ratio, it is beneficial to effectively isolate pollutants or repair skin damage for a long time, while achieving the effects of soothing, repairing and whitening, and comprehensively solving the problems of yellow skin and sensitivity.

[0014] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the multidimensional anti-pollution composition also includes Vaccaria segetalis extract, Adansonia digitata fruit extract and Terminalia chebula fruit extract.

[0015] The inventors of the present application have found that by adding a specific amount of fenugreek extract, baobab pulp extract and terminalia chebula fruit extract, and compounding them with oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract, the present application can further promote the multidimensional anti-pollution composition to resist various pollutants such as air pollution, ultraviolet rays, heavy metals, etc., and comprehensively enhance the soothing, repairing and whitening effects of the multidimensional anti-pollution composition.

[0016] Adding fenugreek extract to the multidimensional anti-pollution composition can enhance the soothing and anti-inflammatory effects of the multidimensional anti-pollution composition, effectively reduce skin redness and allergic reactions, promote skin blood circulation, and help improve the skin's self-repair ability and overall health; adding baobab pulp extract to the multidimensional anti-pollution composition can enhance the multidimensional anti-pollution composition's powerful antioxidant and moisturizing effects, help protect the skin from pollution and ultraviolet damage, and improve skin elasticity and radiance; adding terminalia chebula fruit extract to the multidimensional anti-pollution composition can reduce skin inflammation and improve skin health. The prepared multidimensional anti-pollution composition helps to improve skin firmness and smoothness and reduce skin yellowness.

[0017] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (0.1-3): (0.1-3): (0.01-1).

[0018] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (0.5-2): (0.5-2): (0.05-0.5).

[0019] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (1-1.5): (1-1.5): (0.1-0.2).

[0020] When the cherimoya extract, the cyperus rotundus extract, the baobab pulp extract and the terminalia chebula fruit extract are combined in the above-mentioned specific mass ratio, the multi-dimensional anti-pollution composition achieves better multiple functions such as soothing, repairing and improving skin yellowness. When the cyperus rotundus extract and the baobab pulp extract are excessive, the synergistic effect of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract will be affected.

[0021] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, the preparation method of the Annona chebula extract comprises the following steps:

[0022] The fruit of the custard apple is crushed and sieved, and then added into an ethanol solution for ultrasonic extraction. After the ultrasonic extraction is completed, the supernatant is collected by centrifugation and concentrated to obtain the custard apple extract.

[0023] The study in this application found that the cherimoya extract is rich in flavonoids and has excellent anti-inflammatory, antibacterial and antioxidant activities. Especially when the method provided in this application is used for preparation and extraction, the obtained cherimoya extract can better cooperate with other components to achieve a more excellent comprehensive effect.

[0024] As a preferred embodiment of the multidimensional anti-pollution composition described in the present application, in the ultrasonic extraction, the temperature is 50-65°C, the time is 20-40 minutes, and the power is 100-150W.

[0025] The inventors of the present application conducted a large number of experimental studies on the temperature and power of ultrasonic extraction and found that the use of the temperature and power of ultrasonic extraction within the above-mentioned range of the present application is conducive to improving the extraction efficiency of the custard apple extract, and can more efficiently prepare the custard apple extract with higher purity in a shorter time.

[0026] As a preferred embodiment of the multi-dimensional anti-pollution composition of the present application, the mass percentage of ethanol in the ethanol solution is 55-65%;

[0027] The solid-liquid ratio in ultrasonic extraction is 1g:(10~20)mL.

[0028] The present application uses the ethanol solution in the above-mentioned mass concentration range and the solid-liquid ratio in the above-mentioned range to further increase the content of flavonoids in the custard apple extract, which is beneficial to the application of the custard apple extract in the multidimensional anti-pollution composition.

[0029] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mesh size of the sieving is 60 to 80 meshes.

[0030] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the cherimoya fruit also includes a step of drying to a constant weight at 45-55° C. before crushing.

[0031] The present application also provides the use of the multidimensional anti-pollution composition in the preparation of cosmetics.

[0032] The present application also provides a cosmetic, comprising the above-mentioned multidimensional anti-pollution composition; the mass percentage of the multidimensional anti-pollution composition in the cosmetic is 1 to 20%.

[0033] The inventors of the present application have found that cosmetics prepared with the multidimensional anti-pollution composition, while controlling the multidimensional anti-pollution composition within the above-mentioned mass percentage range, can have a better effect of resisting various pollutants such as air pollution, ultraviolet rays, heavy metals, etc., while achieving a comprehensive effect of soothing, repairing and whitening.

[0034] Preferably, the cosmetics include at least one of lotion, emulsion, cream, mask, essence and spray.

[0035] As a preferred embodiment of the cosmetics described in the present application, the cosmetics are emulsions; the emulsion also includes the following components in mass percentage: 0.05-0.3% thickener, 0.5-1% moisturizer, 0.01-0.3% pH adjuster, 0.5-5% emulsifier and 0.5-3% emulsifier.

[0036] As a preferred embodiment of the cosmetics described in the present application, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0037] The moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0038] The pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium;

[0039] The emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0040] The emulsifier includes at least one of 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate cross-linked polymer.

[0041] Compared with the prior art, this application has the following beneficial effects:

[0042] (1) The multidimensional anti-pollution composition provided by the present application has comprehensive multiple functions. The technical solution of the present application achieves multiple functions such as anti-pollution, anti-oxidation, soothing, repairing and improving skin yellowness by combining oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine, and cherimoya extract, and fenugreek extract, baobab pulp extract and terminalia chebula fruit extract are also added to the composition to enhance the multiple functions. Unlike the skin care products in the prior art that can only address a single problem, the present application provides a comprehensive solution that can more comprehensively address the needs of the skin when facing environmental pollution and sensitive problems.

[0043] (2) The multi-dimensional anti-pollution composition provided in this application is mild and suitable for sensitive skin. The plant extracts (such as Annona oleifera extract and Vaccaria segetalis extract) and acetyl glucosamine used in this application have good mildness and low irritation, which makes the cosmetics of this application more suitable for sensitive skin. In the prior art, many anti-pollution skin care products may irritate sensitive skin, but this solution reduces the risk of irritation and improves the safety of the product by optimizing the selection and ratio of ingredients.

[0044] (3) The multidimensional anti-pollution composition provided by the present application has long-lasting and effective anti-pollution and repair capabilities. The present solution provides long-lasting anti-pollution effects and repair capabilities by using powerful antioxidants such as oxidized resveratrol and epigallocatechin gallate glucoside, as well as other anti-inflammatory and repair ingredients such as baobab pulp extract and terminalia chebula fruit extract. Many products in the prior art may only provide protection for a short period of time, while the technical solution of the present application is committed to achieving long-term and effective protection and improving skin health and appearance. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 This is a comparison chart of the blank application example and application example 15 in effect example 3 before use and after 7 days of use. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical solutions and advantages of the present application, the present application will be further described below in conjunction with specific embodiments.

[0047] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0048] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0049] In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified, and the components and raw materials used in each parallel experiment are of the same type.

[0050] The oxidized resveratrol and acetyl glucosamine used in this application are both conventional commercially available products;

[0051] Epigallocatechin Gallate Glucoside: EGCG, purchased from Givaudan;

[0052] Vaccaria segetalis extract: product number L2-SCB2519, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.;

[0053] Baobab pulp extract: product number realinpioneer3, purchased from Shaanxi Ruilin Panier Biotechnology Co., Ltd.;

[0054] Terminalia chebula fruit extract: item number SXHLHZTQW, purchased from Shaanxi Hengling Natural Biological Products Co., Ltd.

[0055] Annona custardii seed extract: Product No. TK-FLZZ-001, Xi'an Tiankang Biotechnology Co., Ltd.

[0056] The present application provides Annona chebula extracts 1-4.

[0057] The preparation method of the cherimoya extract 1 comprises the following steps:

[0058] The fruit of the custard apple was dried at 50°C to constant weight, then crushed and sieved and added to an ethanol solution (the mass percentage of ethanol is 60%) for ultrasonic extraction. The temperature of the ultrasonic extraction was 55°C, the time was 30min, the power was 120W, and the solid-liquid ratio was 1g:15mL. After the ultrasonic extraction was completed, the supernatant was collected by centrifugation and concentrated to obtain the custard apple extract 1.

[0059] The preparation method of cherimoya extract 2 comprises the following steps:

[0060] The fruit of the custard apple was dried at 50°C to constant weight, then crushed and sieved and added to an ethanol solution (the mass percentage of ethanol is 55%) for ultrasonic extraction. The temperature of the ultrasonic extraction was 50°C, the time was 20 min, the power was 100 W, and the solid-liquid ratio was 1 g:10 mL. After the ultrasonic extraction was completed, the supernatant was collected by centrifugation and concentrated to obtain the custard apple extract 2.

[0061] The preparation method of cherimoya extract 3 comprises the following steps:

[0062] The fruit of the custard apple was dried at 50°C to constant weight, then crushed and sieved and added to an ethanol solution (the mass percentage of ethanol is 65%) for ultrasonic extraction. The temperature of the ultrasonic extraction was 65°C, the time was 40 min, the power was 150 W, and the solid-liquid ratio was 1 g:20 mL. After the ultrasonic extraction was completed, the supernatant was collected by centrifugation and concentrated to obtain the custard apple extract 3.

[0063] The preparation method of cherimoya extract 4 comprises the following steps:

[0064] The fruit of the custard apple was dried at 50°C to constant weight, then crushed and sieved and added to an ethanol solution (the mass percentage of ethanol is 55%) for ultrasonic extraction. The temperature of the ultrasonic extraction was 45°C, the time was 30 min, the power was 90 W, and the solid-liquid ratio was 1 g:15 mL. After the ultrasonic extraction was completed, the supernatant was collected by centrifugation and concentrated to obtain the custard apple extract 4.

[0065] Examples 1 to 17 and Comparative Examples 1 to 19

[0066] Examples 1 to 17 and Comparative Examples 1 to 19 provide a multidimensional anti-pollution composition, whose components and weight portions are shown in Table 1 and Table 2. The preparation method of the multidimensional anti-pollution composition comprises the following steps: weighing the preparation raw materials by weight, mixing them evenly to obtain the multidimensional anti-pollution composition.

[0067] Table 1

[0068]

[0069]

[0070] Table 2

[0071]

[0072]

[0073] Comparative Example 20

[0074] Compared with Example 15, the difference lies in that the composition formula is different, the Annona chebula extract is replaced by the Annona chebula seed extract, and the other components and the preparation method thereof are the same as those in Example 1.

[0075] Effect Example 1: Cell experiment proves that the multi-dimensional anti-pollution composition is anti-pollution

[0076] Experimental methods:

[0077] This effect example explores the anti-air pollution effect of the multi-dimensional anti-pollution composition prepared by Examples 1 to 17 and Comparative Examples 1 to 20.

[0078] The cell line used was human keratinocyte HaCaT (Beina Biotech), and the test conditions were: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%; cell culture and treatment were performed according to the grouping, followed by testing. The specific tests were CYP1A1 and barrier protein FLG gene detection, and inflammatory factor IL-6 content detection. The test methods are as follows:

[0079] (1) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well, 100 μL of culture medium was added to each well, and cultured for 24 h;

[0080] (2) Collect automobile exhaust and expose the cells to the exhaust for 2 h;

[0081] (3) The supernatant was discarded, 100 μL of DMEM medium (2105341, Gibco) was added to the blank control group, and 100 μL of DMEM medium containing 0.01% of the corresponding sample was added to the multidimensional anti-pollution composition prepared in Examples 1 to 17 and Comparative Examples 1 to 20 respectively;

[0082] (4) After incubation for 22 h, the culture medium and cells were collected;

[0083] (5) Detection of CYP1A1 and barrier protein FLG genes: RNA was extracted from cells using an RNA extraction kit (TRIzol, Thermo Fisher), and reverse transcribed into cDNA using a reverse transcription kit (RevertAid RT, Thermo Fisher). The synthesized cDNA was used as a template for real-time fluorescence quantitative PCR (TaqMan, Thermo Fisher). The expression levels of CYP1A1 and FLG genes in HaCaT cells of each group were detected by real-time fluorescence quantitative PCR. GAPDH was used as the internal reference gene. The CYP1A1 primer sequences used were 5'-CTTCATCCTGGAGACCTTCC-3', 5'-AAGACCTCCCAGCGGGCAA-3', and the FLG primer sequences were 5'-TGAAGCCTATGACACCACTGA-3', 5'-TCCCCTACGCTTTCTTGTCCT

[0084] -3', and the GAPDH primer sequences used were 5'-ACTTTGTCAAGCTCATTTCC-3', 5'-TGCAGCGAACTTTATTGATG-3'.

[0085] (6) Detection of inflammatory factor IL-6: A human IL-6 ELISA kit (EHC007.96, Shenzhen Xinbosheng Biotechnology Co., Ltd.) was used to detect the content of inflammatory factor IL-6 released by cells in the culture medium.

[0086] PM2.5 in the air can damage the skin barrier and induce skin inflammation by activating the aromatic hydrocarbon receptor AHR on the skin, which is marked by up-regulation of the aromatic hydrocarbon receptor activation signal CYP1A1 gene expression, reduction of barrier proteins and increase of inflammatory factors. Therefore, after PM2.5 treatment, the lower the CYP1A1 gene expression, the more barrier proteins and the fewer cellular inflammatory factors, the stronger the anti-pollution composition's ability to resist air pollution. The anti-pollution ability of the anti-pollution composition is represented by the improvement of CYP1A1, the barrier protection ability is represented by the improvement of FLG, and the anti-inflammatory ability is represented by the improvement of IL-6. The formula is as follows:

[0087] CYP1A1 improvement rate = 1-CYP1A1 expression 样品处理 / CYP1A1 expression 空白对照 *100%;

[0088] FLG improvement rate = FLG expression 样品处理 / FLG expression 空白对照 *100%-1;

[0089] IL-6 improvement rate = 1-IL-6 content 样品处理 / IL-6 content 空白对照 *100%

[0090] The results are shown in Table 3.

[0091] Table 3

[0092]

[0093]

[0094] Results: It can be seen from Table 3 that the multidimensional anti-pollution compositions prepared in Examples 1 to 17 of the present application have strong anti-pollution ability, barrier protection ability and anti-inflammatory ability, and can effectively isolate pollutants or repair skin damage for a long time.

[0095] Among them, compared with Example 6, Examples 7 to 9 changed the ultrasonic extraction conditions of the custard apple extract, and the anti-pollution effect of the multidimensional anti-pollution composition of Examples 6 to 8 was better than that of Example 9, indicating that the temperature (50 to 65°C) and power (100 to 150W) of the ultrasonic extraction of the present application are beneficial to improving the extraction efficiency of the custard apple extract, and can more efficiently prepare a higher purity custard apple extract in a shorter time, thereby making the anti-pollution ability, barrier protection ability and anti-inflammatory ability of the multidimensional anti-pollution composition better.

[0096] Compared with Examples 1 to 6, Examples 10 to 15 add fenugreek extract, baobab pulp extract and terminalia chebula fruit extract, and are compounded with oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract, which can further promote the multidimensional anti-pollution composition to resist various pollutants such as air pollution, ultraviolet rays, heavy metals, etc., and comprehensively enhance the soothing, repairing and whitening effects of the multidimensional anti-pollution composition.

[0097] Examples 16 to 17 are inferior to Examples 10 to 15, indicating that the specific dosage of Vaccaria extract, Baobab pulp extract and Terminalia chebula fruit extract has a greater impact on the ability to resist air pollution. When an excessive amount of Vaccaria extract and Baobab pulp extract are added to Example 17, the synergistic effect of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and Annona mume extract will be affected. Therefore, the specific dosage of Vaccaria extract and Baobab pulp extract can better improve the anti-air pollution ability of the multi-dimensional anti-pollution composition.

[0098] Compared with Example 15, when one of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and custard apple extract in the multidimensional anti-pollution compositions of Comparative Examples 1 to 4 is removed, the anti-pollution ability, barrier protection ability and anti-inflammatory ability of the composition are not as good as those in Example 15. When the total weight of the components remains unchanged, when at least one of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and custard apple extract is removed from the multidimensional anti-pollution compositions of Comparative Examples 5 to 15, or when custard apple seed extract is used to replace custard apple extract in Comparative Example 20, the air pollution ability is not as good as that in Example 15, which means that when the components of the multidimensional anti-pollution composition of the present application are used in synergistic combination, the anti-pollution ability, barrier protection ability and anti-inflammatory ability of the multidimensional anti-pollution composition can be improved.

[0099] The composition of Comparative Example 16 does not contain Vaccaria segetalis extract and Adansonia digitata pulp extract, and Comparative Example 17 does not contain Terminalia chebula fruit extract. The results are both inferior to those of Example 15, indicating that the addition of Vaccaria segetalis extract, Adansonia digitata pulp extract and Terminalia chebula fruit extract can better improve the anti-air pollution ability of the composition.

[0100] The mass ratio range of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract in Comparative Examples 18 to 19 is not within the scope of the present application, and the anti-air pollution ability of the composition is relatively poor.

[0101] Effect Example 2: Human Skin Patch Test

[0102] The multidimensional anti-pollution compositions prepared in Examples 1 to 17 and Comparative Examples 1 to 20 were formulated into anti-pollution emulsions, as shown in Table 4. The multidimensional anti-pollution compositions in Application Examples 1 to 17 were respectively the multidimensional anti-pollution compositions prepared in Examples 1 to 17; the multidimensional anti-pollution compositions in Comparative Application Examples 1 to 20 were respectively the multidimensional anti-pollution compositions prepared in Comparative Examples 1 to 20; for example, the multidimensional anti-pollution composition used in Application Example 1 was the multidimensional anti-pollution composition in Example 1, the multidimensional anti-pollution composition used in Application Example 8 was the multidimensional anti-pollution composition in Example 8, the multidimensional anti-pollution composition used in Application Example 17 was the multidimensional anti-pollution composition in Example 17, and the same can be said for other applications; the multidimensional anti-pollution composition used in Application Examples 18 to 19 was the multidimensional anti-pollution composition prepared in Example 15.

[0103] Table 4 Anti-pollution emulsion formula

[0104]

[0105]

[0106] The present application provides a method for preparing an anti-pollution emulsion, the preparation method comprising the following steps:

[0107] (1) Mixing a moisturizer, a thickener, and a portion of a pH adjuster (EDTA-disodium) with water, heating to 85° C., homogenizing at a speed of 1300 rpm for 4 minutes, and keeping the mixture warm for later use to obtain a prefabricated component A;

[0108] (2) mixing the emulsifier, heating to 75° C., homogenizing at 1300 rpm for 4 min, and keeping warm for later use after homogenization to obtain prefabricated component B;

[0109] (3) mixing the preservatives and heating to 60° C. to melt to obtain a prefabricated C component;

[0110] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed component C is added at a speed of 300 rpm and stirred and mixed. Then, the temperature is lowered to below 45°C, and the multidimensional anti-pollution composition is added and continued to be stirred for 8 minutes. Finally, the remaining pH adjuster (arginine) is added to adjust the pH to 5.5-7.0, and the stirring can be stopped, and the material is discharged to obtain an anti-pollution emulsion.

[0111] 30 volunteers were recruited, 15 males and 15 females, aged 20-50 years old. The closed patch test method was used. An equal amount (0.020mL-0.025mL) of the test sample (anti-pollution emulsion containing 20% ​​w / w multi-dimensional anti-pollution composition) was placed in a specific patch tester, which was applied to the volunteer's arm with a low-allergenic tape, and gently pressed to evenly apply it on the skin for 24 hours; the blank control group was distilled water, and the blank application example was to use an emulsion without an anti-pollution composition. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours, and 48 hours, and the results were recorded. The level of adverse reactions to the skin is shown in Table 5 below.

[0112] Table 5

[0113]

[0114] After testing, the anti-pollution emulsions containing the multi-dimensional anti-pollution composition provided in Examples 1 to 17 of the present application and Comparative Examples 1 to 20 all showed negative reactions after human patch tests, and are safe and non-irritating to human skin.

[0115] Effect Example 3: Soothing, repairing, and removing yellowness (whitening) human efficacy test

[0116] Experimental method: According to the "Technical Specification for Safety of Cosmetics" (2015), 222 Asian adult testers with sensitive skin aged 18-60 were selected and randomly divided into 37 groups, with 6 people in each group. The volunteers used samples (the samples were anti-pollution emulsions with multi-dimensional anti-pollution compositions according to Table 3) or blank application examples (anti-pollution emulsions without multi-dimensional anti-pollution compositions) on their entire faces once in the morning and evening every day, and visited to collect data on the 0th and 7th days of use. After the visit, the volunteers washed their faces with facial cleansers, sat in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10% for 20 minutes, and then used Visia-7 to take facial photos. The exported red zone pictures were analyzed to obtain the a* value; a skin moisture loss test probe ( The transepidermal water loss (TEWL) of the zygomatic bone was measured by TM Hex; the yellowness b* value of the zygomatic bone was determined by CK skin color test probe CL400.

[0117] The skin soothing ability is represented by the improvement of the a* value, the skin barrier repair ability is represented by the improvement of the TEWL value, and the skin de-yellowing ability is represented by the improvement of the b* value. The formula is as follows: Improvement rate = [(sample day 0 - sample day 7) / sample day 0 - (blank application example day 0 - blank application example day 7) / blank application example day 0] * 100%.

[0118] The results are shown in Table 6.

[0119] Table 6

[0120]

[0121]

[0122] It can be seen from the above table that when the multidimensional anti-pollution composition prepared in the embodiments of the present application is prepared into an anti-pollution emulsion (Application Examples 1 to 19), the a* value improvement rate is more than 20.9%, the TEWL value improvement rate is more than 15.2%, and the b* value improvement rate is more than 5.5%.

[0123] The ultrasonic extraction conditions of the custard apple extract in Application Examples 6 to 9 of the present application are different. The a* value improvement rate, TEWL value improvement rate, and b* value improvement rate of Application Examples 6 to 8 are better than those of Example 9, indicating that the temperature (50 to 65°C) and power (100 to 150W) of the ultrasonic extraction of the present application are beneficial to improving the extraction efficiency of the custard apple extract, so that the prepared anti-pollution emulsion can better achieve multiple functions such as anti-pollution, anti-oxidation, soothing, repairing and improving skin yellowness.

[0124] Application Examples 10 to 17 add Vaccaria extract, Baobab pulp extract and Terminalia chebula fruit extract, and compound with oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and Annona chebula extract to enhance multiple functions such as soothing, repairing and improving skin yellowness. Application Examples 10 to 15 are better than Application Examples 16 to 17, indicating that when Vaccaria extract, Baobab pulp extract and Terminalia chebula fruit extract are matched in a specific mass ratio, the multiple functions of the anti-pollution lotion such as soothing, repairing and improving skin yellowness are better.

[0125] When one of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and custard apple extract in comparative application examples 1 to 4 is removed, or, when the total weight of the components remains unchanged, when at least one of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and custard apple extract in comparative application examples 5 to 15 is removed, or when comparative application example 20 uses custard apple seed extract instead of custard apple extract, the anti-pollution emulsion is not as good as that in application example 15 in terms of soothing, repairing and whitening. This means that the multi-dimensional anti-pollution composition of the present application can only be used when the components of the present application and their specific mass ratios are used in synergistic combination to better improve the product's multiple functions such as soothing, repairing and improving skin yellowness.

[0126] The composition of comparative example 16 does not contain Vaccaria extract and baobab pulp extract, and comparative example 17 does not contain Terminalia chebula fruit extract, and the results are not as good as those of example 15, indicating that the addition of Vaccaria extract, baobab pulp extract and Terminalia chebula fruit extract can better improve the soothing, repairing and whitening effects of the product. The mass ratio range of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract in comparative examples 18 to 19 is not within the scope of this application, and the soothing, repairing and improving skin yellowness effects of the product are poor.

[0127] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application rather than to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, ordinary technicians in this field should understand that the technical solution of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present application.

Claims

1. A multidimensional anti-pollution composition, characterized in that: The multidimensional anti-pollution composition comprises oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract; The mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.1-3): (0.001-0.1): (1-10): (0.1-5).

2. The multidimensional anti-pollution composition according to claim 1, wherein The mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.2-2): (0.005-0.05): (4-8): (0.5-3).

3. The multidimensional anti-pollution composition according to claim 2, wherein The mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.5-1): (0.01-0.02): (6-7): (1-2).

4. The multidimensional anti-pollution composition according to claim 1, wherein The multidimensional anti-pollution composition further comprises a Vaccaria segetalis extract, an Adansonia digitata fruit extract and a Terminalia chebula fruit extract.

5. The multidimensional anti-pollution composition according to claim 4, wherein The mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (0.1-3): (0.1-3): (0.01-1).

6. The multidimensional anti-pollution composition according to claim 5, wherein The mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (0.5-2): (0.5-2): (0.05-0.5).

7. The multidimensional anti-pollution composition according to claim 6, wherein The mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (1-1.5): (1-1.5): (0.1-0.2).

8. The multidimensional anti-pollution composition according to claim 1, wherein The preparation method of the Annona chebula extract comprises the following steps: The cherimoya fruit is crushed and sieved, and then added into an ethanol solution for ultrasonic extraction. After the ultrasonic extraction is completed, the supernatant is collected by centrifugation and concentrated to obtain the cherimoya extract.

9. The multidimensional anti-pollution composition according to claim 8, wherein During the ultrasonic extraction, the temperature is 50-65° C., the time is 20-40 minutes, and the power is 100-150W.

10. The multidimensional anti-pollution composition according to claim 8, wherein In the ethanol solution, the mass percentage of ethanol is 55-65%; The solid-liquid ratio in ultrasonic extraction is 1g:(10~20)mL.

11. Use of the multidimensional anti-pollution composition according to any one of claims 1 to 10 in the preparation of cosmetics.

12. A cosmetic, characterized in that: The multidimensional anti-pollution composition comprises the multidimensional anti-pollution composition according to any one of claims 1 to 10; the mass percentage of the multidimensional anti-pollution composition in the cosmetic is 1 to 20%.

13. The cosmetic according to claim 12, wherein The cosmetic is an emulsion; the emulsion further comprises the following components in percentage by mass: 0.05-0.3% of a thickener, 0.5-1% of a moisturizer, 0.01-0.3% of a pH regulator, 0.5-5% of an emulsifier, and 0.5-3% of a preservative.

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