Liquid gastrointestinal ultrasound examination reagent and preparation method thereof

Abstract

The present invention discloses a liquid gastrointestinal ultrasonic examination reagent and a preparation method thereof. The liquid gastrointestinal ultrasonic examination reagent of the present invention comprises an aqueous medium and germinated barley powder that can form a homogeneous mixture with the aqueous medium. The liquid gastrointestinal ultrasonic examination reagent of the present invention can effectively eliminate the mucus interference in the gastrointestinal cavity, increase the gastrointestinal cavity residence time, prolong the emptying time, provide more ample time for the operator, reduce the waiting time of the patient, improve the inspection accuracy, avoid the preparation process, and eliminate the source of microbial infection. In addition, the liquid gastrointestinal ultrasonic examination reagent of the present invention also has the advantages of being easy to use, easy to drink, absorbable and digestible, having no side effects, and being widely used.

Description

Technical Field

[0001] The invention belongs to the technical field of ultrasonic examination reagents, and in particular relates to a liquid gastrointestinal ultrasonic examination reagent and a preparation method thereof. Background Art

[0002] Among the current medical technologies for diagnosing gastrointestinal diseases, the main gold standards are digestive endoscopy and enhanced CT. However, these two examinations have certain limitations, such as digestive endoscopy has certain medical risks and cannot examine submucosal lesions and external pressure lesions of the digestive tract, and enhanced CT is not flexible enough and has the risk of ionizing radiation.

[0003] Gastrointestinal contrast ultrasound has been widely used in hospitals at all levels across the country and has been clinically recognized. Like other imaging examinations such as CT and MRI, it is considered a good supplementary examination method for gastroscopy. Due to the advantages of ultrasound technology, such as convenience, non-invasiveness, no ionizing radiation, easy acceptance by patients, and high cost-effectiveness, it has become the preferred imaging method for initial screening and follow-up of various gastrointestinal diseases.

[0004] Gastrointestinal ultrasound filling examination refers to an ultrasound examination method in which the patient fills the stomach cavity / colon with oral / enema water, contrast agents or ultrasound contrast agents before the examination to expel the gas in the cavity so that the gastrointestinal cavity is filled and becomes a homogeneous echo area, forming a good acoustic interface contrast, and more clearly showing the hierarchical structure of the stomach wall and intestinal wall, as well as the lesions.

[0005] Gastrointestinal ultrasound filling examination has a wide range of indications and few contraindications. It is a very potential examination method for initial screening and follow-up of gastrointestinal diseases. It is very suitable for pre-endoscopy examinations, physical examinations and examinations in primary hospitals. Its indications include: ① Organic lesions of the gastrointestinal tract: gastritis, inflammatory bowel disease, peptic ulcer, gastrointestinal tumors, polyps, submucosal lesions (such as stromal tumors, lymphomas, neuroendocrine tumors, lipomas, schwannomas, hemangiomas, etc.), hiatal hernia, achalasia, etc.; ② Congenital lesions of the gastrointestinal tract: congenital hypertrophic pyloric stenosis, duplication malformations, ectopic pyloric opening, duodenal atresia, annular pancreas, etc.; ③ Functional lesions of the gastrointestinal tract: gastroesophageal reflux, gastroduodenal reflux, gastroptosis, gastrointestinal dysmotility, etc.; ④ Patients who cannot tolerate gastroscopy or have contraindications to gastroscopy (such as pregnant women, children, and the elderly); ⑤ Preliminary screening of physical examinations of healthy people and people at high risk of gastrointestinal cancer; ⑥ Identification of changes in external pressure of the gastric wall caused by adjacent organs around the gastrointestinal tract (liver, bile duct, pancreas, spleen, kidneys, etc.) or lesions.

[0006] At present, most commonly used gastrointestinal ultrasound contrast aids are powders, which are used according to the instructions of different manufacturers to be mixed into a uniform semi-fluid paste and then taken orally. In order to make the gelatinization process rapid and sufficient, this process needs to be prepared by a dedicated person before the examination. If you want to obtain a contrast aid with good homogeneity that the patient can use immediately, this preparation process usually takes 20-40 minutes. In the time-sensitive outpatient ultrasound examination, this undoubtedly prolongs the time for medical treatment and increases unnecessary conflicts between doctors and patients. In addition, the existing contrast aids are non-sterile products, which may increase the risk of infection from exogenous microorganisms for some special patients. Some products add soy substances, which have a relatively high fat content, and the products are prone to spoilage. Summary of the invention

[0007] In order to solve at least some of the technical problems in the above-mentioned prior art, the present invention prepares highland barley into a liquid ultrasonic gastrointestinal tract examination auxiliary agent or contrast agent through operations such as germination, cooking, drying, crushing, compounding, and sterilization, thereby realizing gastrointestinal tract ultrasonic filling imaging examination while avoiding microbial contamination. In addition, the preparation method of the present invention is simple, and the reagent has the advantages of easy operation and the like. Specifically, the present invention includes the following contents.

[0008] A first aspect of the present invention provides a liquid gastrointestinal ultrasound examination reagent, which includes an aqueous medium and germinated barley powder that can form a homogeneous mixture with the aqueous medium.

[0009] In certain embodiments, according to the liquid gastrointestinal ultrasound examination reagent of the present invention, the germinated barley powder is germinated barley powder that has been dried, crushed to a suitable particle size, and sterilized.

[0010] In certain embodiments, according to the liquid gastrointestinal ultrasound examination reagent of the present invention, the particle size of the germinated barley powder is 60-120 meshes.

[0011] In certain embodiments, the liquid gastrointestinal ultrasound examination reagent according to the present invention further comprises at least one of granules, powders and pastes derived from plant seeds.

[0012] In certain embodiments, the liquid gastrointestinal ultrasound examination reagent according to the present invention further comprises black sesame paste.

[0013] In certain embodiments, according to the liquid gastrointestinal ultrasound examination reagent of the present invention, the soluble solid content of highland barley powder in the liquid gastrointestinal ultrasound examination reagent is 8-12%.

[0014] In certain embodiments, according to the liquid gastrointestinal ultrasound examination reagent of the present invention, the liquid gastrointestinal ultrasound examination reagent is a liquid ultrasonic gastrointestinal examination aid.

[0015] In certain embodiments, according to the liquid gastrointestinal ultrasound examination reagent of the present invention, the defoaming agent contained in the liquid gastrointestinal ultrasound examination reagent does not exceed 5%.

[0016] A second aspect of the present invention provides a method for preparing a liquid gastrointestinal ultrasound examination reagent, comprising the following steps:

[0017] (1) allowing highland barley to germinate to obtain germinated highland barley;

[0018] (2) washing the germinated highland barley to obtain washed germinated highland barley;

[0019] (3) mixing the cleaned sprouted highland barley with seasoning liquid to obtain a mixture;

[0020] (4) subjecting the mixed material to high temperature and high pressure ripening to obtain ripened sprouted barley;

[0021] (5) drying the ripened sprouted highland barley to obtain dried sprouted highland barley;

[0022] (6) grinding the dried germinated barley to obtain germinated barley powder, and mixing the germinated barley powder with an aqueous medium and at least one of a granule, a powder and a paste from plant seeds to form a stable and homogeneous mixture; and

[0023] (7) Sterilizing the mixture.

[0024] In certain embodiments, according to the preparation method of the present invention, the germinated highland barley is dried and crushed to control the particle size of the germinated highland barley powder to be 60-120 mesh, the highland barley powder obtained by drying and crushing is mixed with black sesame paste, and pre-gelatinized to obtain a gelatinized product. In certain embodiments, according to the preparation method of the present invention, the ratio of the germinated highland barley powder obtained by drying and crushing to the black sesame paste is (1-5):1.

[0025] In certain embodiments, according to the preparation method of the present invention, the pre-gelatinization comprises cooking in boiling water for 5-20 min.

[0026] The liquid gastrointestinal ultrasound examination reagent of the present invention increases the gastrointestinal cavity residence time, prolongs the emptying time, provides more ample time for the operator, reduces the patient's waiting time, and improves the examination accuracy. In addition, the liquid gastrointestinal ultrasound examination reagent of the present invention also has the advantages of being easy to use, easy to drink, absorbable and digestible, having no side effects, and being widely used. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The effect of different highland barley varieties on the acoustic imaging of the contrast enhancer in a simulated gastric pouch in vitro is shown.

[0028] Figure 2 The effect of different soluble solid content on the acoustic image of the visualization agent in a simulated gastric pouch in vitro is shown.

[0029] Figure 3 The effects of different preparation methods of germinated barley products on the acoustic imaging of the developer in a simulated gastric pouch in vitro are shown.

[0030] Figure 4 The effect of different preparation processes of liquid developer of germinated barley products on the acoustic image of the developer in a simulated stomach pouch in vitro is shown.

[0031] Figure 5 The comparison of in vitro and in vivo sonograms of the developer sample of the present invention and the control group is shown, wherein the control group is a commercially available developer product.

[0032] Figure 6 A is the effect of different defoaming agents on the intragastric sound image of the contrast aid; B is the effect of different dimethicone defoaming agent contents on the intragastric sound image of the contrast aid.

[0033] Figure 7 A is the effect of enzymatic hydrolysis on the short-line-like strong echo in the stomach of the auxiliary agent; B is the effect of removing the roots of the germination products on the short-line-like strong echo in the stomach of the auxiliary agent.

[0034] Figure 8 This study is about the effect of the particle size of soluble solids in highland barley germination products on the intragastric acoustic imaging of the contrast agent.

[0035] Fig. 9 The invention provides a comparison of in vitro and in vivo sonograms of the liquid ultrasonic gastrointestinal tract examination aid and a control group, wherein the control group is a commercially available aid product. DETAILED DESCRIPTION

[0036] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0037] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that the upper and lower limits of the scope and each intermediate value therebetween are specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0038] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art to which the invention belongs. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0039] Liquid gastrointestinal ultrasound examination reagent

[0040] One aspect of the present invention provides a liquid gastrointestinal ultrasound examination reagent, which includes an aqueous medium and sprouted barley powder that can form a homogeneous mixture with the aqueous medium, wherein the sprouted barley powder can stably exist in the aqueous medium with a certain particle size, and stable existence means that the barley powder with a suitable particle size does not or basically does not settle in the aqueous medium.

[0041] In the present invention, "sprouted highland barley" is the germination product of highland barley seeds, wherein the germination operation can be carried out using techniques known in the art, which is not particularly limited. In a preferred embodiment, the highland barley seeds selected for germination are full, intact endosperm, non-mildewed, and non-worm-eaten highland barley seeds. Preferably, the highland barley seeds are subjected to wet germination (kept at a suitable humidity or soaked in water or nutrient solution) to obtain the germinated highland barley sprouted under moist conditions.

[0042] In the present invention, "aqueous medium" includes but is not limited to distilled water, deionized water, mineral water, purified water or buffer, etc.

[0043] In a preferred embodiment, the germinated highland barley powder is germinated highland barley powder that has been dried, crushed to a suitable particle size, compounded, and sterilized.

[0044] In a preferred embodiment, the liquid gastrointestinal ultrasound examination reagent of the present invention further comprises at least one of granules, powders and pastes from plant seeds. In a specific embodiment, the liquid gastrointestinal ultrasound examination reagent of the present invention further comprises black sesame paste.

[0045] In a preferred embodiment, the liquid gastrointestinal ultrasound examination reagent of the present invention is a liquid gastrointestinal ultrasound examination aid.

[0046] In a preferred embodiment in which the liquid gastrointestinal ultrasound examination reagent is a liquid ultrasonic gastrointestinal examination developer, the particle size of the germinated barley powder is 60-120 mesh, preferably 60-110 mesh, also preferably 60-100 mesh, further preferably 60-90 mesh, more preferably 60-80 mesh, for example 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80 mesh.

[0047] In a preferred embodiment in which the liquid gastrointestinal ultrasound examination reagent is a liquid ultrasonic gastrointestinal examination developer, the soluble solids content of highland barley powder in the liquid gastrointestinal ultrasound examination reagent of the present invention is 8-12%, for example, 8%, 9%, 10%, 11%, 12%.

[0048] In a preferred embodiment in which the liquid gastrointestinal ultrasound examination reagent is a liquid ultrasonic gastrointestinal examination aid, in the liquid gastrointestinal ultrasound examination reagent of the present invention, the ratio of the germinated barley powder to the black sesame paste is (1-5):1, for example, 1:1, 2:1, 3:1, 4:1, 5:1.

[0049] The liquid gastrointestinal ultrasound examination reagent of the present invention may further include a defoamer. When the defoamer is included, the content of the defoamer is no more than 5%, preferably no more than 4%, and more preferably no more than 3%, for example, no more than 2%, 1%, or 0.01%. The specific type of the defoamer is not particularly limited, and may be a conventional defoamer used in the art, such as a dimethicone defoamer.

[0050] When the product of the present invention is a liquid ultrasonic gastrointestinal tract examination aid, considering that the gastrointestinal tract has the characteristics of digesting and absorbing food, irregular shape, high acidity and high alkalinity cavity environment, gastrointestinal peristalsis, etc., the gastrointestinal ultrasound filling examination is highly dependent on the aid, and a qualified aid needs to be able to tolerate gastric acid and intestinal fluid, empty slowly under gastrointestinal peristalsis to provide an ultrasonic examination time window, and not be quickly decomposed in gastrointestinal digestive enzymes. After research, the aid prepared by the present invention using sprouted barley has a long residence time in the gastrointestinal cavity and is slowly emptied, so that the operator has ample time to perform the operation and inspection; in addition, it has the advantages of being easy to drink, absorbable and digestible, and having no side effects.

[0051] The ultrasound contrast enhancer of the present invention appears as a uniform high / equal echo interface on the sonogram, and has a significant contrast effect with the weak echo interface of the gastrointestinal wall; it has a good effect of eliminating mucus interference in the gastrointestinal cavity, does not produce acoustic artifacts, and avoids the modulation step of the contrast enhancer, thereby increasing the number of gastrointestinal ultrasound filling examinations in the ultrasound department and expanding the scope of the examination population (including special populations such as the elderly, pregnant women and children); by increasing the contrast between the contrast enhancer and the gastrointestinal wall, gastrointestinal tract-related lesions are displayed more clearly, the diagnostic accuracy of gastrointestinal tract-related diseases is increased, the initial screening work for clinical work is done well, and the positive detection rate of digestive endoscopy is improved; at the same time, the present invention can achieve the purpose of examination without increasing the infection risk of high-risk patients through the sterilization operation of the contrast enhancer.

[0052] The ultrasonic developer of the present invention has the following characteristics in terms of acoustic properties: ① the longitudinal wave velocity value is between 1500-1580 m / s; ② the acoustic impedance is between 1.58-1.70 Z (106 Pa*s / m); ③ the acoustic attenuation coefficient = 1; ④ the slope of the acoustic attenuation coefficient is between 0.7-1.3 dB / (cmMHz); ⑤ the viscosity is similar to that of digestive tract mucus; ⑥ the pH value is between 7.3-7.4.

[0053] Preparation method

[0054] One aspect of the present invention provides a method for preparing a liquid gastrointestinal ultrasound examination reagent, comprising the following steps:

[0055] (1) allowing highland barley to germinate to obtain germinated highland barley;

[0056] (2) washing the germinated highland barley to obtain washed germinated highland barley;

[0057] (3) mixing the cleaned sprouted highland barley with seasoning liquid to obtain a mixture;

[0058] (4) subjecting the mixed material to high temperature and high pressure ripening to obtain ripened sprouted barley;

[0059] (5) drying the ripened sprouted highland barley to obtain dried sprouted highland barley;

[0060] (6) grinding the dried germinated barley to obtain germinated barley powder, and mixing the germinated barley powder with an aqueous medium and at least one of a granule, a powder and a paste from plant seeds to form a stable and homogeneous mixture; and

[0061] (7) Sterilizing the mixture.

[0062] In the present invention, the seasoning liquid is an aqueous solution including seasonings, and examples of seasonings include but are not limited to sodium chloride, glucose, sucrose, vitamins, essences, etc. The volume ratio of the sprouted barley to the seasoning liquid is 1:(1-5), such as 1:1, 1:2, 1:3, 1:4, 1:5.

[0063] In the present invention, high temperature and high pressure aging includes treating at 100-140°C, preferably 120-130°C, for example 120°C, 121°C, 122°C, 123°C, 124°C, 125°C, 126°C, 127°C, 128°C, 129°C, 130°C for 5-30 min, preferably 10-25 min, also preferably 10-20 min, for example 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 min.

[0064] In the present invention, the drying and pulverizing operations can be performed using techniques known in the art and are not particularly limited.

[0065] In a preferred embodiment of the preparation method, the present invention controls the particle size of the sprouted highland barley powder so that it forms a stable and homogeneous mixture with an aqueous medium and at least one of a granule, powder and paste from plant seeds. Wherein, the particle size of the sprouted highland barley powder is controlled to be 60-120 mesh, preferably 60-110 mesh, also preferably 60-100 mesh, further preferably 60-90 mesh, more preferably 60-80 mesh, such as 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80 mesh. In a specific embodiment, the present invention controls the particle size of the sprouted highland barley powder so that it forms a stable and homogeneous mixture with an aqueous medium and black sesame paste.

[0066] In a preferred embodiment of the preparation method, the present invention dries and grinds the germinated barley to control the particle size of the germinated barley powder, mixes the obtained germinated barley powder with black sesame paste, and performs pre-gelatinization to obtain a gelatinized product, wherein the ratio of the germinated barley powder obtained by drying and grinding to the black sesame paste is (1-5):1, for example, 1:1, 2:1, 3:1, 4:1, 5:1; the pre-gelatinization includes cooking in boiling water for 5-20 min, preferably 5-19 min, also preferably 5-18 min, further preferably 5-17 min, more preferably 5-16 min, more preferably 5-15 min, for example 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 min.

[0067] In the present invention, the sterilization temperature is 120-140°C, preferably 121-130°C, for example 121°C, 122°C, 123°C, 124°C, 125°C, 126°C, 127°C, 128°C, 129°C, 130°C, and the sterilization time is 5-30 min, preferably 7-28 min, also preferably 9-26 min, further preferably 12-25 min, more preferably 15-25 min, for example 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 min.

[0068] Example 1

[0069] The following is a method for preparing a liquid gastrointestinal ultrasound contrast agent.

[0070] Select full, intact endosperm, mildew-free, and insect-free highland barley seeds, wash away impurities and dust with tap water, lay the cleaned seeds flat and put them into a spray germination machine for germination, take them out after germination, wash the germinated highland barley, prepare them according to a material-water ratio of 1:1, and sterilize them at 121°C for 15 min. Dry the product, crush it after drying, control the particle size to 60-120 mesh, mix highland barley powder and black sesame paste at a ratio of 7:3, prepare them according to a material-liquid ratio of 1:12 after mixing, pre-gelatinize (cook in boiling water for 10 min), and after pre-gelatinization, package them, sterilize them at 121°C for 15 min, and the resulting product is a liquid dosage form gastrointestinal contrast agent.

[0071] Example 2

[0072] The following shows the effects of different factors on the acoustic imaging of the visualization agent in a simulated gastric pouch in vitro.

[0073] 1.1 Effects of different highland barley varieties on the acoustic imaging of the contrast enhancer in the simulated stomach pouch in vitro

[0074] Based on the differences in dietary fiber, β-glucan, amylose, protein and other components of different highland barley varieties, as well as differences in tolerance to gastric acid, the acoustic properties of three varieties, No. 1 Highland Barley, No. 2 Highland Barley and No. 3 Highland Barley, in an in vitro simulated gastric pouch were tested with the same soluble solid content, preparation method of germinated highland barley products and preparation process of liquid developer for germinated highland barley products to determine the appropriate highland barley variety.

[0075] like Figure 1 As shown in the results, the study found that there was no significant difference in the acoustic characteristics of highland barley No. 1, No. 2, and No. 3 in the simulated stomach pouch in vitro, and relatively uniform isoechoic images could be obtained. The highland barley variety had no significant effect on the ultrasonic imaging effect.

[0076] 1.2 Effects of different soluble solid contents on the acoustic imaging of the visualization agent in a simulated gastric pouch in vitro

[0077] By testing the germinated barley products with different soluble solid contents, their acoustic properties in a simulated stomach pouch in vitro, and their liquid stability, whether precipitation occurs, and the effect of mixing after precipitation on their acoustic properties, the germinated barley products with a soluble solid content that meets the acoustic properties of the developer are determined.

[0078] like Figure 2 As shown, through research, it was found that different highland barley varieties with a soluble solid content of 3%-8% have good liquid stability in an in vitro simulated stomach pouch, are not prone to precipitation, and mixing after precipitation has no significant effect on its acoustic properties.

[0079] 1.3 Effects of different preparation methods of germinated barley products on the acoustic imaging of the developer in the simulated stomach pouch in vitro

[0080] By detecting the acoustic characteristics of different preparation methods of germinated barley products (including dry barley germinated products and fresh / moist barley germinated products) in a simulated stomach pouch in vitro, the appropriate preparation method of the germinated barley product is determined.

[0081] like Figure 3 As shown, the study found that the moist highland barley germination product has better homogeneity than the dry highland barley germination product in the simulated stomach pouch in vitro, and a more uniform isoechoic image can be obtained.

[0082] 1.4 Effects of preparation processes of different germinated barley products liquid developer on the acoustic image of developer in simulated stomach pouch in vitro

[0083] By testing the acoustic characteristics of different preparation processes of liquid developer for germinated barley products (including ultrafine grinding, filtration, shearing, homogenization and other processes) in a simulated stomach pouch in vitro, the appropriate preparation process of liquid developer for germinated barley products was determined.

[0084] like Figure 4 As shown, the study found that the wet highland barley germination product that was ultrafinely crushed, filtered through 200 meshes, sheared, and homogenized had better homogeneity in an in vitro simulated stomach pouch, and a more uniform isoechoic image could be obtained.

[0085] Example 3

[0086] The following shows the effects of different factors on the intragastric ultrasound imaging of contrast-enhancing agents.

[0087] 1.1 Complete the preparation and debugging of in vivo gastrointestinal ultrasound contrast agent

[0088] Based on the preliminary screening of in vitro gastrointestinal ultrasound aids, we obtained the aid samples of wet highland barley germination products through ultrafine grinding, 200 mesh filtration, shearing and homogenization for further screening and debugging in vivo. We orally administered 500-1000 ml of the aid samples to test the ultrasound aid ability of the aid in the stomach.

[0089] like Figure 5 As shown in the figure, through research, it was found that the contrast agent with relatively uniform sound image in the simulated stomach pouch in vitro had uneven echo in the stomach, with many point and line strong echoes. As the time in the stomach increased, small pieces of strong echoes would float and be layered. The stomach wall of some contrast agents was not clearly displayed, and there were changes in the posterior echo. Therefore, debugging was carried out according to the above problems.

[0090] Based on the assumption that the dot-line-like strong echoes in the intragastric contrast agent may be bubbles, short fibers of barley rhizomes, or protein clots generated after contact with gastric acid, the following study was conducted in this example.

[0091] 1.2 Effect of defoaming agents on the intragastric ultrasound of the contrast enhancer

[0092] By testing the effects of different defoamers and defoamer contents on the acoustic properties of the contrast agent in the stomach, it is determined whether the uneven strong echoes are caused by small bubbles.

[0093] like Figure 6 As shown in A, the effects of different defoaming agents on the intragastric acoustic image of the contrast aid were first tested, and it was found that dimethicone defoaming agents had the best defoaming effect. In addition, all defoaming agents would cause an increase in gastric fundus gas and frequent burping after taking them.

[0094] Subsequently, the effects of different contents of dimethicone antifoaming agents on the intragastric sonographic image of the contrast aid were examined, such as Figure 6 As shown in B, the study found that when the content of dimethicone antifoaming agent exceeds 5%, the viscosity of the auxiliary agent will increase, and the echo in the stomach will increase and become uneven. Therefore, the content of dimethicone antifoaming agent should not exceed 5%.

[0095] like Figure 7 As shown in Figure A, adding thermostable α-amylase to the contrast agent has no significant effect on the short-line-like strong echoes in the stomach. Figure 7 As shown in B, after removing the root of the germination product, the short-line strong echo of the contrast agent in the stomach was significantly reduced. Therefore, the short-line strong echo of the contrast agent in the stomach is caused by the fiber at the root of the germination product. These fibers are thin and can pass through 200 mesh filtration, causing the sound image of short-line strong echo in the stomach. Removing the root can effectively reduce the short-line strong echo and increase the uniformity of the sound image of the contrast agent.

[0096] 1.3 Effect of solid particle size in highland barley germination products on the intragastric ultrasound image of contrast agents

[0097] The contrast agent showed stratification and increased posterior echo after half an hour in the stomach, which may be caused by the small particle size of the soluble solid in the contrast agent, because the small particle size of the highland barley germination product can be quickly decomposed by gastric acid, resulting in the phenomenon of reduced liquid stability. Therefore, this embodiment adjusted the particle size of the highland barley germination product. Figure 8 As shown, contrast agents with particle sizes between 60-80 mesh have the most uniform echo in the stomach.

[0098] 1.4 Comparison of in vitro and in vivo sonograms of the developer product of this example and the control product

[0099] The results are as follows Fig. 9 As shown, through two repeated double-blind experiments, a new type of contrast enhancer with uniform in vivo and in vitro sonograms was finally obtained, and there was no significant difference compared with competing products.

[0100] In this embodiment, the control group is a gastric window acoustic contrast agent (production batch number: 230804) produced by Hangzhou Huagong Benxiang Pharmaceutical Technology Co., Ltd.

[0101] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some of the technical features therein by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A liquid gastrointestinal ultrasound examination reagent that does not require preparation, characterized in that: The invention comprises an aqueous medium, black sesame paste and sprouted barley powder, wherein the sprouted barley powder has a soluble solid content of 3-8%, and the ratio of the sprouted barley powder to the black sesame paste is (1-5):

1. The sprouted barley powder is dried, crushed to a particle size of 60-80 meshes, sterilized, and the root fibers are removed. The liquid gastrointestinal ultrasound examination reagent is prepared by the following method: wet germination of highland barley to obtain germinated highland barley; washing the germinated highland barley to obtain washed germinated highland barley; mixing the washed germinated highland barley with water to obtain a mixture; maturing the mixture at 100-140°C to obtain maturated germinated highland barley; drying the maturated germinated highland barley to obtain dried germinated highland barley; pulverizing the dried germinated highland barley to obtain germinated highland barley powder, mixing the germinated highland barley with an aqueous medium and black sesame paste, and pre-gelatinizing to form a stable and homogeneous mixture; and sterilizing the mixture at 120-140°C.

2. The liquid gastrointestinal ultrasound examination reagent that does not require preparation according to claim 1, characterized in that: The liquid gastrointestinal ultrasound examination reagent is a liquid gastrointestinal ultrasound examination auxiliary agent.

3. The method for preparing the liquid gastrointestinal ultrasound examination reagent that does not require preparation according to claim 1, characterized in that: It includes the following steps: (1) wet germination of highland barley to obtain germinated highland barley; (2) washing the germinated highland barley to obtain washed germinated highland barley; (3) mixing the cleaned germinated highland barley with water to obtain a mixed material; (4) ripening the mixture at 100-140° C. to obtain ripened sprouted barley; (5) drying the ripened sprouted highland barley to obtain dried sprouted highland barley; (6) grinding the dried sprouted barley to obtain sprouted barley powder with a particle size of 60-80 mesh, mixing the sprouted barley powder with an aqueous medium and black sesame paste, and pre-gelatinizing the mixture to form a stable and homogeneous mixture; (7) Sterilize the mixture at 120-140°C.

4. The preparation method according to claim 3, characterized in that The pre-gelatinization includes cooking in boiling water for 5-20 minutes.

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