A degraded desert grassland seed reseeding formula and application method

Abstract

The invention discloses a degraded desert grassland seed reseeding formula and application method, and belongs to the technical field of seed reseeding formula preparation. The degraded desert grassland seed reseeding formula is composed of the following components in parts by weight: 50-70 parts of forage seeds, 10-20 parts of composite fertilizer, 2-5 parts of nanocomposite agent, 2-4 parts of sodium polyacrylate, 3-6 parts of plant growth regulator, 35-50 parts of fermented cow dung, wherein the forage seeds are fixedly combined with Stipa, Artemisia frigida, Kochia scoparia, Agropyron sphaerocephala, Lespedeza serrata, and Caragana korshinskii; the composite fertilizer is prepared from corn stalks, acrylic acid, paraformaldehyde, urea and citric acid, and the nanocomposite agent is prepared from 4-triphenylamine borate, 5-bromo-2-thiophene carboxaldehyde, 2,4-dimethylpyrrole, triethylamine, N-bromosuccinimide, divaleryl diboron and caffeic acid phenylethyl ester. The formula prepared by the method can effectively improve the recovery of desert grassland.

Description

Technical Field

[0001] The invention belongs to the technical field of seed reseeding formula preparation, and in particular relates to a degraded desert grassland seed reseeding formula and an application method. Background Art

[0002] The study found that the coverage of degraded natural grasslands has been significantly reduced, the sandiness of the soil has increased, and the sand fixation capacity has been greatly weakened, which is one of the key causes of frequent sandstorms. Therefore, restoring degraded grasslands is not only an urgent need to maintain my country's northern ecological barrier, but also an inevitable choice to promote regional sustainable development.

[0003] Among the many restoration technologies, reseeding technology has become an important means of ecological restoration of degraded grasslands due to its simple operation and significant effect. The development process of reseeding technology is a transition from reseeding of a single grass species to reseeding of a variety of native grass species, from scientific optimization of reseeding by tillage to reseeding by no-tillage, and from the application of a single technology to the coordinated application of multiple technologies such as reseeding, mowing, and fertilization. These advances have not only greatly improved the efficiency of ecological restoration of degraded grasslands, but also significantly enhanced grassland species diversity, system stability, and ecological service functions.

[0004] Patent CN115044153A discloses a seed dressing agent for reseeding in desert grassland ecological restoration, the components of which include 4-12 parts of marine polysaccharides, 0.06-0.08 parts of vitamin A, 0.04-0.07 parts of vitamin C, 7-14 parts of sodium polyacrylate, 12-16 parts of super absorbent resin A, 8-14 parts of super absorbent resin B, 2-7 parts of lactic acid bacteria, 4-6 parts of yeast, 1-3 parts of ethylamine, 2-5 parts of acrylamide, and 0.7-0.9 parts of phosphoric acid. By adding super absorbent resin A and super absorbent resin B, the water retention rate of the restored grassland is high, thereby improving the germination rate of the grassland, which is beneficial to the restoration of the desert grassland. However, the reseeding seed dressing agent prepared by this method still has room for improvement in terms of seed germination and water absorption and water retention performance. Summary of the invention

[0005] The purpose of the present invention is to provide a degraded desert grassland seed reseeding formula and application method, which are used to solve the technical problems of low seed germination rate and poor water absorption and water retention performance of fertilizers in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] The invention provides a seed reseeding formula for degraded desert grasslands. The formula consists of the following components in parts by weight: 50-70 parts of forage seeds, 10-20 parts of compound fertilizer, 2-5 parts of nanocomposite agent, 2-4 parts of sodium polyacrylate, 3-6 parts of plant growth regulator, and 35-50 parts of fermented cow dung, wherein the forage seeds are composed of a fixed combination of Stipa, Artemisia frigida, and Kochia scoparia, and are combined with one or more of Polygonum multiflorum, Icegrass, Lespedeza, and Caragana; the compound fertilizer is prepared from corn stalks, acrylic acid, N,N'-methylenebisacrylamide, paraformaldehyde, urea, and citric acid; and the nanocomposite agent is prepared from 4-triphenylamine borate, 5-bromo-2-thiophenecarboxaldehyde, 2,4-dimethylpyrrole, triethylamine, N-bromosuccinimide, diboryl boron, and caffeic acid phenethyl ester.

[0008] Preferably, the method for preparing the compound fertilizer comprises the following steps:

[0009] Q1: Wash corn stalks with deionized water, dry, sieve, extract with Soxhlet, and dry to obtain pretreated corn stalks, add them to a sodium hydroxide solution and heat them in a water bath, obtain modified corn stalks after heating, add the modified corn stalks into a container, cool, introduce nitrogen, add potassium persulfate to react, then add acrylic acid and N,N'-methylenebisacrylamide in sequence, stir to react, take out the product, shear, wash, soak, dry, extract with Soxhlet, continue washing, and dry to obtain a composite gel;

[0010] Q2: Add paraformaldehyde to a container containing potassium hydroxide, then add urea, heat to react, and obtain hydroxymethyl urea. Add hydroxymethyl urea to a container containing deionized water and citric acid, heat to react, and after the reaction is completed, add composite gel and deionized water in sequence, continue to react, and after the reaction is completed, dry to obtain compound fertilizer.

[0011] In the above process, corn stalks are first subjected to Soxhlet alkali extraction and then added to a sodium hydroxide solution for alkalization treatment to obtain modified corn stalks. Subsequently, potassium persulfate is used as an initiator, acrylic acid is used as a monomer, and N,N'-methylenebisacrylamide is used as a cross-linking agent to prepare a composite gel. Then, polyformaldehyde and urea undergo a condensation reaction under alkaline conditions to generate hydroxymethyl urea. Finally, the hydroxymethyl urea and the composite gel undergo a chemical reaction to obtain a compound fertilizer.

[0012] Preferably, in Q1, the sieve aperture is 60-80 mesh, the mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 is used for Soxhlet extraction for 20-24 hours, the drying temperature is 60-70°C, the amount ratio of pretreated corn stalks and sodium hydroxide solution is (1-2) g: (100-200) mL, the mass fraction of sodium hydroxide solution is 20wt%, the water bath heating temperature is 60-70°C, the time is 8-10 hours, the temperature is lowered to 20-30°C, nitrogen is introduced for 30-45 minutes, and the modified corn stalks are The dosage ratio of rice straw, potassium persulfate, acrylic acid and N,N'-methylenebisacrylamide is (1-2) g: (0.03-0.05) g: (2-4) g: (0.01-0.02) g, potassium persulfate is added to react for 10-20 minutes, the stirring reaction time is 2-4 hours, washed with deionized water, soaked with anhydrous ethanol for 5-8 hours, extracted with acetone for 10-12 hours, the Soxhlet extraction temperature is 60-70°C, washed with deionized water, and dried at 70-80°C.

[0013] Preferably, in Q2, the purity of paraformaldehyde is 99.0%, the mass ratio of paraformaldehyde, potassium hydroxide and urea is (10-15): (11.67-16.3): (10-12), the heating reaction temperature is 80-90°C, the time is 1-2h, the amount ratio of hydroxymethyl urea, citric acid and composite gel is (10-12) g: (0.5-1.5) mL: (20-25) g, the heating reaction temperature is 60-70°C, the time is 10-20min, the continued reaction temperature is 60-70°C, the time is 10-20min, and the drying temperature is 50-70°C.

[0014] Preferably, the method for preparing the nanocomposite comprises the following steps:

[0015] S1: Add 4-boric acid triphenylamine to tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 5-bromo-2-thiophenecarboxaldehyde, potassium carbonate and tetrakis(triphenylphosphine)palladium in sequence, and perform hydrothermal reaction. After the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate and purify to obtain a yellow solid;

[0016] S2: Add the yellow solid to dichloromethane, stir to dissolve, then add iodine and 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, extract, dry and filter after the reaction to obtain an oily liquid, then add the oily liquid to a container containing dichloromethane, add triethylamine, boron trifluoride ether and dichlorodicyanobenzoquinone in sequence, heat and reflux to react, wash, dry, filter and purify after the reaction to obtain a red solid 1;

[0017] S3: Add red solid 1 and N-bromosuccinimide to dichloromethane in sequence, stir to react, concentrate, and purify to obtain red solid 2; add divaleryl diboron to a container containing dichloromethane, stir to dissolve, add caffeic acid phenethyl ester, stir, then drop boron trifluoride ether, stir to react, extract and wash, dry, filter, and rotary evaporate to obtain an orange-yellow liquid, add the orange-yellow liquid to 1,4-dioxane to dissolve, add red solid 2, potassium carbonate and tetrakis(triphenylphosphine)palladium, hydrothermally react, dry, dissolve, extract and wash, dry, filter, and purify to obtain a nanocomposite.

[0018] In the above process, the synthesis reaction formula of the nanocomposite is as follows:

[0019]

[0020] The results of mass spectrometry analysis of the yellow solid are: m / z: 355.10 (100.0%), 356.11 (25.1%), 357.10(4.6%), 357.11 (3.4%), 356.10 (1.2%), 358.10 (1.1%); the results of mass spectrometry analysis of the red solid 1 are: m / z: 573.22 (100.0%), 574.23 (40.0%), 572.23 (24.8%), 573.23 (9.7%),575.23 (7.1%), 575.22 (5.7%), 574.22 (3.0%), 576.22 (1.7%), 576.23 (1.0%); the results of mass spectrometry analysis of the red solid 2 are: m / z: 731.04 (100.0%), 733.04 (53.0%), 732.04 (52.3%),729.04 (50.0%), 730.04 (25.6%), 734.04 (21.1%), 730.05 (20.0%), 731.05(13.1%), 728.05 (12.4%), 733.05 (11.8%), 729.05 (4.8%), 735.05 (3.6%), 732.05(2.7%), 735.03 (2.1%), 734.05 (2.0%); the results of mass spectrometry analysis of the orange-yellow liquid were: m / z: 420.19(100.0%), 419.20 (51.2%), 421.19 (24.9%), 420.20 (12.7%), 418.20 (6.2%),422.20 (4.5%), 421.20 (2.7%); the results of mass spectrometry analysis of the nanocomposite were: m / z: 945.22 (100.0%),943.22 (97.0%), 944.23 (66.8%), 946.22 (59.2%), 942.23 (51.4%), 944.22(50.6%), 945.23 (44.8%), 943.23 (33.8%), 947.23 (17.0%), 946.23 (12.9%),947.22 (6.6%), 941.23 (6.0%), 948.23 (3.9%), 948.22 (2.5%), 945.24 (2.3%), 947.24 (1.7%).

[0021] Preferably, in S1, the amount ratio of 4-boric acid triphenylamine, tetrahydrofuran, 5-bromo-2-thiophenecarboxaldehyde, potassium carbonate and tetrakis(triphenylphosphine)palladium is (0.5-0.8) g: (25-40) mL: (0.29-0.55) g: (0.4-0.7) g: (0.008-0.012) g, the hydrothermal reaction temperature is 90-110° C., and the time is 10-12 h; in S2, the amount ratio of yellow solid, dichloromethane, iodine and 2,4-dimethylpyrrole is (0.15-0.45) g : (10-15) mL: (0.004-0.006) g: (0.2-0.6) g, the microwave reaction power is 300-500 W, the temperature is 30-35 ° C, the interval reaction is 80-100 s, the dosage ratio of oily liquid, dichloromethane, triethylamine, boron trifluoride ether and dichlorodicyanobenzoquinone is (10-15) mL: (50-80) mL: (2-4) mL: (2-3.5) mL: (0.08-0.15) g, the heating reaction temperature is 40-50 ° C, and the time is 6-8h.

[0022] Preferably, in S3, the amount ratio of the red solid 1, N-bromosuccinimide and dichloromethane is (0.28-0.5) g: (0.2-0.5) g: (10-15) mL, and the stirring reaction time is 2-4 h; the amount ratio of bisvaleryl diboron, dichloromethane, caffeic acid phenethyl ester and boron trifluoride ether is (0.25-0.5) g: 10 mL: (0.2-0.4) g: (0.5-0.6) mL L, stirring time is 20-30min, stirring reaction time is 30-45min, the dosage ratio of orange-yellow liquid, 1,4-dioxane, red solid 2, potassium carbonate and tetrakis(triphenylphosphine)palladium is (2-4)mL: (5-8)mL: (0.6-0.8)g: (0.05-0.06)g: (0.08-0.1)g, the hydrothermal reaction temperature is 110-130℃, and the reaction time is 10-12h.

[0023] Preferably, the method for preparing the degraded desert grassland seed reseeding formula comprises the following steps:

[0024] Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture;

[0025] Step 2: Mix the seed dressing mixture with forage seeds to obtain a formula for seed reseeding in degraded desert grasslands.

[0026] Preferably, the application method of the degraded desert steppe seed reseeding formula is applied to the desert steppe ecological environment.

[0027] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0028] The present invention adds the prepared compound fertilizer and nanocomposite agent to the degraded desert grassland seed reseeding formula. The addition of the compound fertilizer can effectively improve the water absorption and water retention performance of the fertilizer, thereby promoting the germination rate of forage grass seeds. The addition of the nanocomposite agent can effectively kill and inhibit pathogens on the surface of forage grass seeds and in the soil, such as Escherichia coli. The presence of the antibacterial effect can significantly reduce the incidence of diseases, improve the germination rate and survival rate of seeds, and at the same time can also improve the stress resistance of seeds, so that the seeds can still maintain a high germination rate and growth potential under adverse environmental conditions, thereby facilitating the restoration of desert grasslands. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Embodiment 1: This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0031] Q1: The corn stalks were washed with deionized water, dried, passed through an 80-mesh sieve, and extracted with a mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 for 24 hours, and dried at 60°C to obtain pretreated corn stalks. 1.5 g of the pretreated corn stalks were added to 150 mL of a 20 wt% sodium hydroxide solution and heated in a water bath at 70°C for 10 hours. After heating, modified corn stalks were obtained. 1.5 g of the modified corn stalks were added to a container, cooled to 20°C, nitrogen was introduced for 45 minutes, 0.04 g of potassium persulfate was added to react for 10 minutes, and then 3 g of acrylic acid and 0.015 g of N,N'-methylenebisacrylamide were added in sequence, stirred for reaction for 2 hours, the product was taken out, sheared, washed with deionized water, soaked in anhydrous ethanol for 8 hours, dried, extracted with acetone at 70°C for 12 hours, washed with deionized water, and dried at 80°C to obtain a composite gel.

[0032] Q2: Add 12.5g of polyformaldehyde with a purity of 99.0% to a container containing 14.2g of potassium hydroxide, then add 11g of urea, heat at 90℃ for 2h to obtain hydroxymethyl urea, add 11g of hydroxymethyl urea to a container containing 5mL of deionized water and 1mL of citric acid, heat at 60℃ for 15min, after the reaction, add 22.5g of composite gel and 15mL of deionized water in turn, continue to react at 60℃ for 20min, after the reaction, dry at 70℃ to obtain compound fertilizer.

[0033] This embodiment discloses a method for preparing a nanocomposite, comprising the following steps:

[0034] S1: Add 0.65 g of 4-boric acid triphenylamine to 32.5 mL of tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 0.45 g of 5-bromo-2-thiophenecarboxaldehyde, 0.55 g of potassium carbonate and 0.01 g of tetrakis(triphenylphosphine)palladium in sequence, and hydrothermally react at 90° C. for 12 h. After the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate, and purify to obtain a yellow solid;

[0035] S2: Add 0.3 g of yellow solid to 12.5 mL of dichloromethane, stir to dissolve, then add 0.005 g of iodine and 0.4 g of 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, 500 W, 30 ° C interval reaction for 90 s, after the reaction is completed, extract, dry, filter, and obtain an oily liquid, then add 12.5 mL of the oily liquid to a container containing 75 mL of dichloromethane, add 3 mL of triethylamine, 2.75 mL of boron trifluoride ether and 0.12 g of dichlorodicyanobenzoquinone in sequence, heat and reflux at 50 ° C for 8 h, after the reaction is completed, wash, dry, filter, and purify to obtain a red solid 1;

[0036] S3: 0.39 g of red solid 1 and 0.35 g of N-bromosuccinimide were added to 12.5 mL of dichloromethane in sequence, stirred for reaction for 4 h, concentrated, and purified to obtain a red solid 2; 0.37 g of bis(pentanoyl)diboron was added to a container containing 10 mL of dichloromethane, stirred and dissolved, 0.3 g of caffeic acid phenethyl ester was added, stirred for 30 min, and then 0.55 mL of boron trifluoride ether was added dropwise, stirred and reacted for 45 min, extracted and washed, dried, filtered, and rotary evaporated to obtain an orange-yellow liquid, 3 mL of the orange-yellow liquid was added to 6.5 mL of 1,4-dioxane to dissolve, 0.7 g of red solid 2, 0.055 g of potassium carbonate and 0.09 g of tetrakis(triphenylphosphine)palladium were added, and the reaction was hydrothermally reacted at 120°C for 12 h, dried, dissolved, extracted and washed, dried, filtered, and purified to obtain a nanocomposite.

[0037] The present embodiment discloses a seed reseeding formula for degraded desert grassland, which is composed of the following ingredients in parts by weight: 60 parts of a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds, 15 parts of compound fertilizer, 3.5 parts of nanocomposite agent, 3 parts of sodium polyacrylate, 4.5 parts of plant growth regulator, and 42 parts of fermented cow dung.

[0038] This embodiment discloses a method for preparing a seed reseeding formula for degraded desert grassland, comprising the following steps:

[0039] Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture;

[0040] Step 2: Mix the seed dressing mixture with a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds to obtain a formula for seed reseeding in degraded desert steppes.

[0041] Embodiment 2: This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0042] Q1: The corn stalks were washed with deionized water, dried, passed through an 80-mesh sieve, and extracted with a mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 for 24 hours, and dried at 60°C to obtain pretreated corn stalks. 1 g of the pretreated corn stalks was added to 200 mL of a 20 wt% sodium hydroxide solution and heated in a water bath at 70°C for 10 hours. After the heating, modified corn stalks were obtained. 1 g of the modified corn stalks was added to a container, cooled to 20°C, nitrogen was introduced for 45 minutes, 0.03 g of potassium persulfate was added to react for 10 minutes, and then 2 g of acrylic acid and 0.01 g of N,N'-methylenebisacrylamide were added in sequence, stirred for reaction for 2 hours, the product was taken out, sheared, washed with deionized water, soaked in anhydrous ethanol for 8 hours, dried, extracted with acetone at 70°C for 12 hours, washed with deionized water, and dried at 80°C to obtain a composite gel.

[0043] Q2: Add 10g of 99.0% pure paraformaldehyde to a container containing 11.67g of potassium hydroxide, then add 10g of urea, heat at 90℃ for 2h to obtain hydroxymethyl urea, add 10g of hydroxymethyl urea to a container containing 5mL of deionized water and 0.5mL of citric acid, heat at 60℃ for 15min, after the reaction, add 25g of composite gel and 15mL of deionized water in turn, continue to react at 60℃ for 20min, after the reaction, dry at 70℃ to obtain compound fertilizer.

[0044] This embodiment discloses a method for preparing a nanocomposite, comprising the following steps:

[0045] S1: Add 0.5 g of 4-boric acid triphenylamine to 25 mL of tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 0.29 g of 5-bromo-2-thiophenecarboxaldehyde, 0.4 g of potassium carbonate and 0.008 g of tetrakis(triphenylphosphine)palladium in sequence, and hydrothermally react at 90° C. for 12 h. After the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate, and purify to obtain a yellow solid;

[0046] S2: Add 0.15 g of yellow solid to 10 mL of dichloromethane, stir to dissolve, then add 0.006 g of iodine and 0.6 g of 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, 500 W, 30 ° C interval reaction for 90 s, after the reaction is completed, extract, dry, filter to obtain an oily liquid, then add 15 mL of the oily liquid to a container containing 50 mL of dichloromethane, add 2 mL of triethylamine, 2 mL of boron trifluoride ether and 0.08 g of dichlorodicyanobenzoquinone in sequence, heat at 50 ° C for reflux reaction for 8 h, after the reaction is completed, wash, dry, filter, purify to obtain a red solid 1;

[0047] S3: 0.28g of red solid 1 and 0.2g of N-bromosuccinimide were added to 15mL of dichloromethane in sequence, stirred for reaction for 4h, concentrated, and purified to obtain red solid 2; 0.25g of bis(pentanoyl)diboron was added to a container containing 10mL of dichloromethane, stirred and dissolved, 0.2g of caffeic acid phenethyl ester was added, stirred for 30min, and then 0.5mL of boron trifluoride ether was added dropwise, stirred for reaction for 45min, extracted and washed, dried, filtered, and rotary evaporated to obtain an orange-yellow liquid, 2mL of the orange-yellow liquid was added to 8mL of 1,4-dioxane to dissolve, 0.6g of red solid 2, 0.05g of potassium carbonate and 0.08g of tetrakis(triphenylphosphine)palladium were added, hydrothermally reacted at 120°C for 12h, dried, dissolved, extracted and washed, dried, filtered, and purified to obtain a nanocomposite.

[0048] The present embodiment discloses a seed reseeding formula for degraded desert grassland, which is composed of the following ingredients in parts by weight: 50 parts of a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds, 10 parts of compound fertilizer, 5 parts of nanocomposite agent, 2 parts of sodium polyacrylate, 3 parts of plant growth regulator, and 35 parts of fermented cow dung.

[0049] This embodiment discloses a method for preparing a seed reseeding formula for degraded desert grassland, comprising the following steps:

[0050] Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture;

[0051] Step 2: Mix the seed dressing mixture with a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds to obtain a formula for seed reseeding in degraded desert steppes.

[0052] Embodiment 3: This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0053] Q1: The corn stalks were washed with deionized water, dried, passed through an 80-mesh sieve, and extracted with a mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 for 24 hours, and dried at 60°C to obtain pretreated corn stalks. 2g of pretreated corn stalks were added to 100mL of a 20wt% sodium hydroxide solution and heated in a water bath at 70°C for 10 hours. After heating, modified corn stalks were obtained. 2g of modified corn stalks were added to a container, cooled to 20°C, nitrogen was introduced for 45 minutes, 0.05g of potassium persulfate was added to react for 10 minutes, and then 4g of acrylic acid and 0.02g of N,N'-methylenebisacrylamide were added in sequence, stirred for reaction for 2 hours, the product was taken out, sheared, washed with deionized water, soaked in anhydrous ethanol for 8 hours, dried, extracted with acetone at 70°C for 12 hours, washed with deionized water, and dried at 80°C to obtain a composite gel.

[0054] Q2: Add 15g of polyformaldehyde with a purity of 99.0% to a container containing 16.3g of potassium hydroxide, then add 12g of urea, heat at 90℃ for 2h to obtain hydroxymethyl urea, add 12g of hydroxymethyl urea to a container containing 5mL of deionized water and 1.5mL of citric acid, heat at 60℃ for 15min, after the reaction, add 20g of composite gel and 15mL of deionized water in turn, continue to react at 60℃ for 20min, after the reaction, dry at 70℃ to obtain compound fertilizer.

[0055] This embodiment discloses a method for preparing a nanocomposite, comprising the following steps:

[0056] S1: Add 0.8 g of 4-boric acid triphenylamine to 40 mL of tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 0.55 g of 5-bromo-2-thiophenecarboxaldehyde, 0.7 g of potassium carbonate and 0.012 g of tetrakis(triphenylphosphine)palladium in sequence, and hydrothermally react at 90° C. for 12 h. After the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate, and purify to obtain a yellow solid;

[0057] S2: Add 0.45 g of yellow solid to 15 mL of dichloromethane, stir to dissolve, then add 0.004 g of iodine and 0.2 g of 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, 500 W, 30 ° C interval reaction for 90 s, after the reaction is completed, extract, dry, filter, and obtain an oily liquid, then add 10 mL of the oily liquid to a container containing 80 mL of dichloromethane, add 4 mL of triethylamine, 3.5 mL of boron trifluoride ether and 0.15 g of dichlorodicyanobenzoquinone in sequence, heat and reflux at 50 ° C for 8 h, after the reaction is completed, wash, dry, filter, and purify to obtain a red solid 1;

[0058] S3: 0.5 g of red solid 1 and 0.5 g of N-bromosuccinimide were added to 10 mL of dichloromethane in sequence, stirred for reaction for 4 h, concentrated, and purified to obtain a red solid 2; 0.5 g of bis(pentanoyl)diboron was added to a container containing 10 mL of dichloromethane, stirred and dissolved, 0.4 g of caffeic acid phenethyl ester was added, stirred for 30 min, and then 0.6 mL of boron trifluoride ether was added dropwise, stirred for reaction for 45 min, extracted and washed, dried, filtered, and rotary evaporated to obtain an orange-yellow liquid, 4 mL of the orange-yellow liquid was added to 5 mL of 1,4-dioxane to dissolve, 0.8 g of red solid 2, 0.06 g of potassium carbonate and 0.1 g of tetrakis(triphenylphosphine)palladium were added, hydrothermally reacted at 120°C for 12 h, dried, dissolved, extracted and washed, dried, filtered, and purified to obtain a nanocomposite.

[0059] The present embodiment discloses a seed reseeding formula for degraded desert grassland, which is composed of the following ingredients in parts by weight: 70 parts of a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds, 20 parts of compound fertilizer, 2 parts of nanocomposite agent, 4 parts of sodium polyacrylate, 6 parts of plant growth regulator, and 50 parts of fermented cow dung.

[0060] This embodiment discloses a method for preparing a seed reseeding formula for degraded desert grassland, comprising the following steps:

[0061] Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture;

[0062] Step 2: Mix the seed dressing mixture with a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds to obtain a formula for seed reseeding in degraded desert steppes.

[0063] Embodiment 4: This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0064] Q1: The corn stalks were washed with deionized water, dried, passed through an 80-mesh sieve, and extracted with a mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 for 24 hours, and dried at 60°C to obtain pretreated corn stalks. 1.2 g of the pretreated corn stalks were added to 120 mL of a 20 wt% sodium hydroxide solution and heated in a water bath at 70°C for 10 hours. After heating, modified corn stalks were obtained. 1.2 g of the modified corn stalks were added to a container, cooled to 20°C, nitrogen was introduced for 45 minutes, 0.03 g of potassium persulfate was added to react for 10 minutes, and then 2.2 g of acrylic acid and 0.017 g of N,N'-methylenebisacrylamide were added in sequence, stirred for reaction for 2 hours, the product was taken out, sheared, washed with deionized water, soaked in anhydrous ethanol for 8 hours, dried, extracted with acetone at 70°C for 12 hours, washed with deionized water, and dried at 80°C to obtain a composite gel.

[0065] Q2: Add 11g of polyformaldehyde with a purity of 99.0% to a container containing 12.4g of potassium hydroxide, then add 10.5g of urea, heat at 90℃ for 2h to obtain hydroxymethyl urea, add 10.5g of hydroxymethyl urea to a container containing 5mL of deionized water and 0.7mL of citric acid, heat at 60℃ for 15min, after the reaction, add 21g of composite gel and 15mL of deionized water in turn, continue to react at 60℃ for 20min, after the reaction, dry at 70℃ to obtain compound fertilizer.

[0066] This embodiment discloses a method for preparing a nanocomposite, comprising the following steps:

[0067] S1: Add 0.6 g of 4-boric acid triphenylamine to 30 mL of tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 0.31 g of 5-bromo-2-thiophenecarboxaldehyde, 0.5 g of potassium carbonate and 0.009 g of tetrakis(triphenylphosphine)palladium in sequence, and hydrothermally react at 90° C. for 12 h. After the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate, and purify to obtain a yellow solid;

[0068] S2: Add 0.2 g of yellow solid to 11 mL of dichloromethane, stir to dissolve, then add 0.006 g of iodine and 0.3 g of 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, 500 W, 30 ° C interval reaction for 90 s, after the reaction is completed, extract, dry, filter, and obtain an oily liquid, then add 11 mL of the oily liquid to a container containing 60 mL of dichloromethane, add 2.5 mL of triethylamine, 2.1 mL of boron trifluoride ether and 0.09 g of dichlorodicyanobenzoquinone in sequence, heat and reflux at 50 ° C for 8 h, after the reaction is completed, wash, dry, filter, and purify to obtain a red solid 1;

[0069] S3: 0.32g of red solid 1 and 0.25g of N-bromosuccinimide were added to 11mL of dichloromethane in sequence, stirred for reaction for 4h, concentrated, and purified to obtain red solid 2; 0.3g of bis(pentanoyl)diboron was added to a container containing 10mL of dichloromethane, stirred and dissolved, 0.25g of caffeic acid phenethyl ester was added, stirred for 30min, and then 0.52mL of boron trifluoride ether was added dropwise, stirred and reacted for 45min, extracted and washed, dried, filtered, and rotary evaporated to obtain an orange-yellow liquid, 2.5mL of the orange-yellow liquid was added to 6mL of 1,4-dioxane to dissolve, 0.65g of red solid 2, 0.052g of potassium carbonate and 0.083g of tetrakis(triphenylphosphine)palladium were added, hydrothermally reacted at 120°C for 12h, dried, dissolved, extracted and washed, dried, filtered, and purified to obtain a nanocomposite.

[0070] The present embodiment discloses a seed reseeding formula for degraded desert grassland, which is composed of the following ingredients in parts by weight: 55 parts of a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds, 12 parts of compound fertilizer, 3 parts of nanocomposite agent, 3.5 parts of sodium polyacrylate, 4 parts of plant growth regulator, and 35 parts of fermented cow dung.

[0071] This embodiment discloses a method for preparing a seed reseeding formula for degraded desert grassland, comprising the following steps:

[0072] Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture;

[0073] Step 2: Mix the seed dressing mixture with a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds to obtain a formula for seed reseeding in degraded desert steppes.

[0074] Embodiment 5: This embodiment discloses a method for preparing a compound fertilizer, comprising the following steps:

[0075] Q1: The corn stalks were washed with deionized water, dried, passed through an 80-mesh sieve, and extracted with a mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 for 24 hours, and dried at 60°C to obtain pretreated corn stalks. 1.7 g of the pretreated corn stalks were added to 180 mL of a 20 wt% sodium hydroxide solution and heated in a water bath at 70°C for 10 hours. After heating, modified corn stalks were obtained. 1.7 g of the modified corn stalks were added to a container, cooled to 20°C, nitrogen was introduced for 45 minutes, 0.05 g of potassium persulfate was added to react for 10 minutes, and then 2.8 g of acrylic acid and 0.012 g of N,N'-methylenebisacrylamide were added in sequence, stirred for reaction for 2 hours, the product was taken out, sheared, washed with deionized water, soaked in anhydrous ethanol for 8 hours, dried, extracted with acetone at 70°C for 12 hours, washed with deionized water, and dried at 80°C to obtain a composite gel.

[0076] Q2: Add 14 g of polyformaldehyde with a purity of 99.0% to a container containing 15.8 g of potassium hydroxide, then add 11.5 g of urea, heat at 90°C for 2 h to obtain hydroxymethyl urea, add 11.5 g of hydroxymethyl urea to a container containing 5 mL of deionized water and 1.2 mL of citric acid, heat at 60°C for 15 min, after the reaction is completed, add 24 g of composite gel and 15 mL of deionized water in sequence, continue to react at 60°C for 20 min, after the reaction is completed, dry at 70°C to obtain compound fertilizer.

[0077] This embodiment discloses a method for preparing a nanocomposite, comprising the following steps:

[0078] S1: Add 0.7 g of 4-boric acid triphenylamine to 35 mL of tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 0.48 g of 5-bromo-2-thiophenecarboxaldehyde, 0.6 g of potassium carbonate and 0.011 g of tetrakis(triphenylphosphine)palladium in sequence, and hydrothermally react at 90° C. for 12 h. After the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate, and purify to obtain a yellow solid;

[0079] S2: Add 0.3 g of yellow solid to 14 mL of dichloromethane, stir to dissolve, then add 0.004 g of iodine and 0.5 g of 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, 500 W, 30 ° C interval reaction for 90 s, after the reaction is completed, extract, dry, filter, and obtain an oily liquid, then add 14 mL of the oily liquid to a container containing 70 mL of dichloromethane, add 3.5 mL of triethylamine, 3.2 mL of boron trifluoride ether and 0.11 g of dichlorodicyanobenzoquinone in sequence, heat and reflux at 50 ° C for 8 h, after the reaction is completed, wash, dry, filter, and purify to obtain a red solid 1;

[0080] S3: 0.45g of red solid 1 and 0.45g of N-bromosuccinimide were added to 14mL of dichloromethane in sequence, stirred for reaction for 4h, concentrated, and purified to obtain red solid 2; 0.4g of bis(pentanoyl)diboron was added to a container containing 10mL of dichloromethane, stirred and dissolved, 0.35g of caffeic acid phenethyl ester was added, stirred for 30min, and then 0.58mL of boron trifluoride ether was added dropwise, stirred and reacted for 45min, extracted and washed, dried, filtered, and rotary evaporated to obtain an orange-yellow liquid, 3.5mL of the orange-yellow liquid was added to 7mL of 1,4-dioxane to dissolve, 0.75g of red solid 2, 0.058g of potassium carbonate and 0.091g of tetrakis(triphenylphosphine)palladium were added, hydrothermally reacted at 120°C for 12h, dried, dissolved, extracted and washed, dried, filtered, and purified to obtain a nanocomposite.

[0081] The present embodiment discloses a seed reseeding formula for degraded desert grassland, which is composed of the following ingredients in parts by weight: 65 parts of a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds, 18 parts of compound fertilizer, 4 parts of nanocomposite agent, 2.5 parts of sodium polyacrylate, 5 parts of plant growth regulator, and 45 parts of fermented cow dung.

[0082] This embodiment discloses a method for preparing a seed reseeding formula for degraded desert grassland, comprising the following steps:

[0083] Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture;

[0084] Step 2: Mix the seed dressing mixture with a mixture of Stipa, Artemisia frigida, Kochia scoparia and Lespedeza seeds to obtain a formula for seed reseeding in degraded desert steppes.

[0085] Comparative Example 1: Compared with Example 1, in the preparation process of the compound fertilizer in Comparative Example 1, no composite gel is added, and other conditions remain unchanged.

[0086] Comparative Example 2: Compared with Example 1, in the preparation process of the nanocomposite agent in Comparative Example 2, no 4-triphenylamine borate was added, and other conditions remained unchanged.

[0087] Comparative Example 3: Compared with Example 1, in Comparative Example 3, during the preparation of the degraded desert steppe seed reseeding formula, no compound fertilizer is added, and other conditions remain unchanged.

[0088] Comparative Example 4: Compared with Example 1, in the preparation process of the degraded desert steppe seed reseeding formula in Comparative Example 4, no nanocomposite agent is added, and other conditions remain unchanged.

[0089] Experimental example: Sow the obtained samples, observe regularly, add water, control temperature, increase light, regularly record the number of germinated seeds, calculate the germination rate, and take 10 seedlings 20 days after sowing to measure the plant height. The test results are shown in Table 1:

[0090]

[0091] From the test results in Table 1, it can be seen that the degraded desert grassland seed reseeding formula prepared in Examples 1-5 of the present invention has excellent germination rate and plant height. From the comparison between Comparative Example 1 and Examples 1-5, it can be seen that the addition of composite gel can improve the germination rate and plant height of seeds; from the comparison between Comparative Example 2 and Examples 1-5, it can be seen that the addition of 4-boric acid triphenylamine can improve the germination rate and plant height of seeds; from the comparison between Comparative Example 3 and Examples 1-5, it can be seen that the addition of compound fertilizer can improve the germination rate and plant height of seeds; from the comparison between Comparative Example 4 and Examples 1-5, it can be seen that the addition of nanocomposite can improve the germination rate and plant height of seeds.

[0092] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0093] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A seed reseeding formula for degraded desert grassland, characterized in that: The invention comprises the following components in parts by weight: 50-70 parts of forage seeds, 10-20 parts of compound fertilizer, 2-5 parts of nanocomposite agent, 2-4 parts of sodium polyacrylate, 3-6 parts of plant growth regulator, and 35-50 parts of fermented cow dung, wherein the forage seeds are composed of a fixed combination of Stipa, Artemisia frigida, and Kochia scoparia, and are combined with one or more of Polygonum multiflorum, Ice grass, Lespedeza, and Caragana; the compound fertilizer is prepared from corn stalks, acrylic acid, N,N'-methylenebisacrylamide, paraformaldehyde, urea, and citric acid; the nanocomposite agent is prepared from 4-triphenylamine borate, 5-bromo-2-thiophenecarboxaldehyde, 2,4-dimethylpyrrole, triethylamine, N-bromosuccinimide, diboryl diboron, and caffeic acid phenethyl ester. The preparation method of the compound fertilizer comprises the following steps: Q1: Wash corn stalks with deionized water, dry, sieve, extract with Soxhlet, and dry to obtain pretreated corn stalks, add them into sodium hydroxide solution and heat them in a water bath, and after heating, obtain modified corn stalks, add the modified corn stalks into a container, cool, introduce nitrogen, add potassium persulfate to react, then add acrylic acid and N,N'-methylenebisacrylamide in sequence, stir to react, take out the product, shear, wash, soak, dry, extract with Soxhlet, continue washing, and dry to obtain a composite gel, the amount ratio of pretreated corn stalks and sodium hydroxide solution is (1-2) g: (100-200) mL, the mass fraction of sodium hydroxide solution is 20wt%, and the amount ratio of modified corn stalks, potassium persulfate, acrylic acid and N,N'-methylenebisacrylamide is (1-2) g: (0.03-0.05) g: (2-4) g: (0.01-0.02) g; Q2: Add paraformaldehyde to a container containing potassium hydroxide, then add urea, heat to react, and obtain hydroxymethyl urea. Add hydroxymethyl urea to a container containing deionized water and citric acid, heat to react, and after the reaction is completed, add composite gel and deionized water in sequence, and continue to react. After the reaction is completed, dry to obtain composite fertilizer. The mass ratio of paraformaldehyde, potassium hydroxide and urea is (10-15): (11.67-16.3): (10-12), and the dosage ratio of hydroxymethyl urea, citric acid and composite gel is (10-12) g: (0.5-1.5) mL: (20-25) g; The preparation method of the nanocomposite comprises the following steps: S1: Add 4-boric acid triphenylamine to tetrahydrofuran, stir and dissolve under nitrogen atmosphere, then add 5-bromo-2-thiophenecarboxaldehyde, potassium carbonate and tetrakis(triphenylphosphine)palladium in sequence, hydrothermally react, and after the reaction is completed, redissolve, extract, wash, dry, filter, rotary evaporate and purify to obtain a yellow solid. The amount ratio of 4-boric acid triphenylamine, tetrahydrofuran, 5-bromo-2-thiophenecarboxaldehyde, potassium carbonate and tetrakis(triphenylphosphine)palladium is (0.5-0.8) g: (25-40) mL: (0.29-0.55) g: (0.4-0.7) g: (0.008-0.012) g; S2: Add the yellow solid to dichloromethane, stir to dissolve, then add iodine and 2,4-dimethylpyrrole, stir and place in a microwave synthesizer for microwave reaction, after the reaction is completed, extract, dry, filter, and obtain an oily liquid, then add the oily liquid to a container containing dichloromethane, add triethylamine, boron trifluoride ether and dichlorodicyanobenzoquinone in sequence, heat and reflux to react, after the reaction is completed, wash, dry, filter, and purify to obtain a red solid 1, yellow The usage ratio of solid, dichloromethane, iodine and 2,4-dimethylpyrrole is (0.15-0.45) g: (10-15) mL: (0.004-0.006) g: (0.2-0.6) g, and the usage ratio of oily liquid, dichloromethane, triethylamine, boron trifluoride etherate and dichlorodicyanobenzoquinone is (10-15) mL: (50-80) mL: (2-4) mL: (2-3.5) mL: (0.08-0.15) g; S3: Add red solid 1 and N-bromosuccinimide to dichloromethane in sequence, stir to react, concentrate, and purify to obtain red solid 2; add divaleryl diboron to a container containing dichloromethane, stir to dissolve, add caffeic acid phenethyl ester, stir, then drop boron trifluoride ether, stir to react, extract and wash, dry, filter, and rotary evaporate to obtain an orange-yellow liquid, add the orange-yellow liquid to 1,4-dioxane to dissolve, add red solid 2, potassium carbonate and tetrakis(triphenylphosphine)palladium, hydrothermally react, dry, dissolve, extract and wash, dry, filter, and purify to obtain a nanocomposite, red solid 1, N- The dosage ratio of bromosuccinimide and dichloromethane is (0.28-0.5) g: (0.2-0.5) g: (10-15) mL, the dosage ratio of bis(pentanoyl)diboron, dichloromethane, caffeic acid phenethyl ester and boron trifluoride ether is (0.25-0.5) g: 10 mL: (0.2-0.4) g: (0.5-0.6) mL, and the dosage ratio of orange-yellow liquid, 1,4-dioxane, red solid 2, potassium carbonate and tetrakis(triphenylphosphine)palladium is (2-4) mL: (5-8) mL: (0.6-0.8) g: (0.05-0.06) g: (0.08-0.1) g.

2. The degraded desert grassland seed reseeding formula according to claim 1, characterized in that: In the Q1, the sieve aperture is 60-80 mesh, and the mixed solution of anhydrous ethanol and benzene in a volume ratio of 1:1 is used for Soxhlet extraction for 20-24 hours, the drying temperature is 60-70°C, the water bath heating temperature is 60-70°C, the time is 8-10 hours, the temperature is lowered to 20-30°C, nitrogen is introduced for 30-45 minutes, potassium persulfate is added for reaction for 10-20 minutes, the stirring reaction time is 2-4 hours, the mixture is washed with deionized water, soaked in anhydrous ethanol for 5-8 hours, and extracted with acetone for 10-12 hours. The Soxhlet extraction temperature is 60-70°C, and the mixture is continued to be washed with deionized water, and the drying temperature is 70-80°C.

3. The degraded desert grassland seed reseeding formula according to claim 1, characterized in that: In the Q2, the purity of paraformaldehyde is 99.0%, the heating reaction temperature is 80-90°C, the time is 1-2h, the heating reaction temperature is 60-70°C, the time is 10-20min, the continued reaction temperature is 60-70°C, the time is 10-20min, and the drying temperature is 50-70°C.

4. The degraded desert grassland seed reseeding formula according to claim 1, characterized in that: In the S1, the hydrothermal reaction temperature is 90-110°C and the time is 10-12h; in the S2, the microwave reaction power is 300-500W, the temperature is 30-35°C, the interval reaction is 80-100s, the heating reaction temperature is 40-50°C and the time is 6-8h.

5. The degraded desert grassland seed reseeding formula according to claim 1, characterized in that: In S3, the stirring reaction time is 2-4 hours; the stirring time is 20-30 minutes, the stirring reaction time is 30-45 minutes, the hydrothermal reaction temperature is 110-130° C., and the time is 10-12 hours.

6. The degraded desert steppe seed reseeding formula according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: Step 1: mixing and stirring the compound fertilizer, the nanocomposite agent, sodium polyacrylate, the plant growth regulator and the fermented cow dung to obtain a seed dressing mixture; Step 2: Mix the seed dressing mixture with forage seeds to obtain a formula for seed reseeding in degraded desert grasslands.

7. The application method of the seed reseeding formula for degraded desert grassland is characterized by: The degraded desert steppe seed reseeding formula described in any one of claims 1 to 6 is applied to the desert steppe ecological environment.

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