Swab sampling tube containing reagent and swab sampling set
By using isolation membrane separating chambers in the swab sampling tube to store antibiotics and buffers, and using cotton swabs to squeeze the retained disease material, the problem of buffers needing to be prepared and uneven virus residues in the prior art is solved, and rapid Mix and effectively retain effects.
Patent Information
- Application Number
- CN202411660673.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-20
- Publication Date
- 2025-01-07
AI Technical Summary
The buffer in the existing swab sampling tube needs to be prepared and used immediately, and the viruses remaining on the cotton swab after sampling are difficult to be evenly distributed in the buffer, resulting in inconvenience in use and inefficiency.
A swab sampling tube containing reagents was designed. By setting an isolation membrane in the tube body, the antibiotic dry powder and buffer are stored respectively, and the cotton swab is inserted and poked the isolation membrane to make it Mix, reduce configuration time, and squeeze the swab through the isolation hole to retain disease.
It realizes rapid mixing of buffers and effective retention of disease materials, simplifies the sampling process, and improves sampling efficiency and safety.
Smart Images
Figure CN119257645A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of biotechnology instruments, and more particularly to a swab sampling tube containing reagents and a swab sampling kit. Background Art
[0002] Currently, the test tubes used to collect poultry throat and anal swabs are EP tubes, and the buffer solution is usually double-antibiotic glycerol PBS solution. Due to the characteristics of antibiotics, they need to be used as soon as possible after adding antibiotics. This limitation requires the solution to be prepared and used immediately.
[0003] Sampling is usually done with a sterile cotton swab. After sampling, the cotton swab is immersed in a buffer solution and cannot be evenly operated, so that a large amount of virus is still on the cotton swab head and cannot be well homogenized into the buffer solution. Summary of the invention
[0004] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a swab sampling tube containing a reagent, which is used to solve the technical problems that the sampling swab buffer needs to be prepared and used immediately and the virus remaining on the cotton swab after sampling cannot be better retained in the buffer.
[0005] The above technical objectives of the present invention are achieved through the following technical solutions:
[0006] A swab sampling tube containing reagents comprises a tube body for containing reagents to be mixed for use, a tube cover for preventing the reagents from leaking to the outside of the tube body is threadedly connected to the top of the tube body, a funnel portion for separating the inner cavity of the tube body into a first cavity and a second cavity is sealed inside the tube body, an isolation hole for inserting a cotton swab from the first cavity into the second cavity is opened on the funnel portion, and an isolation membrane is arranged on the isolation hole; the reagents comprise antibiotic dry powder located in the first cavity and a buffer located in the second cavity; the diameter of the isolation hole is 1-3 mm larger than the diameter of the cotton swab.
[0007] Optionally, a sleeve hole for fixing a cotton swab is provided inside the tube cover.
[0008] Optionally, anti-slip grooves are provided on the side walls of the tube cover.
[0009] Optionally, the isolation film is medical grade aluminum foil.
[0010] Optionally, the tube body is made of PP material.
[0011] Optional, brown material tube for the tube body.
[0012] Optionally, the bottom of the tube body is conical.
[0013] Optionally, the outer wall of the tube body is provided with a plurality of capacity scale lines.
[0014] Another object of the present invention is to provide a swab sampling kit, which includes the aforementioned swab sampling tube containing reagents and a cotton swab.
[0015] In summary, the above embodiments of the present invention have the following beneficial effects: by providing a first cavity and a second cavity separated by an isolation membrane in the tube body, antibiotic dry powder and buffer solution are respectively and independently placed, and when in use, a cotton swab is used to pierce the isolation membrane, so that the antibiotic dry powder and the buffer solution are mixed, thereby reducing the buffer solution preparation time, so that sampling can be carried out at any time. In addition, the volume of the cotton swab after sampling will increase after being immersed in water, so when the cotton swab is dragged out, it can be squeezed through the isolation hole, so that more of the sampled diseased material can be retained in the sampling tube. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 is a perspective view of the present invention;
[0017] Figure 2 is an exploded view of the present invention;
[0018] Figure 3 is a partial cross-sectional view of the tube body of the present invention;
[0019] Figure 4 yes Figure 3 Magnified view of area A. DETAILED DESCRIPTION
[0020] In order to make the purpose, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings. Several embodiments of the present invention are shown in the accompanying drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.
[0021] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances. The terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.
[0022] The present invention is described in detail below in conjunction with the accompanying drawings and embodiments.
[0023] The present invention provides a swab sampling tube containing a reagent, such as Figure 1-4 As shown, it includes a tube body 1 for accommodating reagents that need to be mixed for use, a tube cover 2 is threadedly connected to the top of the tube body 1 for preventing the reagents from leaking to the outside of the tube body 1, and a funnel portion 13 is sealed inside the tube body 1 to separate the inner cavity of the tube body 1 into a first cavity 11 and a second cavity 12, and an isolation hole 131 is opened on the funnel portion 13 for inserting a cotton swab 4 from the first cavity 11 to the second cavity 12, and an isolation membrane 3 is arranged on the isolation hole 131; the reagents include antibiotic dry powder located in the first cavity 11 and a buffer located in the second cavity 12; the diameter of the isolation hole 131 is 1-3 mm larger than the diameter of the cotton swab 4.
[0024] In this embodiment, the antibiotic dry powder is a double-antibiotic powder, the buffer solution is a PBS glycerol mixture, the tube body 1 and the tube cover 2 are both made of transparent PP material, and the tube cover 2 is fixed to the top of the tube body 1 by threaded connection. Among them, the tube body 1 is composed of an inner tube 14 and an outer tube 15. The inner tube 14 is filled with antibiotic dry powder, and the outer tube 15 is filled with buffer solution. The inner tube 14 and the outer tube 15 are assembled into a whole by existing technology to form the tube body 1, such as setting matching threads on the bottom side wall of the inner tube 14 and the top side wall of the outer tube 15, first turning and tightening them together, and then ultrasonic welding is performed at the dividing line to make the inner tube 14 and the outer tube 15 sealed and connected. The isolation membrane 3 is fixed to the bottom of the funnel 13 by hot melting or ultrasonic welding, etc., to block the isolation hole 131. The diameter of the isolation hole 131 is 1-3 mm larger than the diameter of the straight rod part (non-cotton part) of the cotton swab 4. This diameter difference enables the cotton swab 4 to easily bring the double-antibody powder into the second cavity 12. When taking out the cotton swab 4, the isolation hole 131 can also better squeeze the cotton swab head.
[0025] The preparation order of the swab sampling tube is as follows: fix the isolation membrane 3 on the isolation hole 131 of the funnel part 13 of the inner tube 14 by hot melting or ultrasonic welding, add an appropriate amount of PBS glycerol mixture into the outer tube 15, and then tighten the inner tube 14 on the outer tube 15 to achieve preliminary fixation, and then ultrasonically weld the connection between the inner tube 14 and the outer tube 15 to obtain a tube body 1 with good sealing performance; then add the double-antibody powder into the inner tube 14, and finally snap the tube cover 2 onto the tube body 1 and tighten it to achieve the effect of encapsulating the reagent.
[0026] The steps for using the swab sampling tube are as follows: unscrew the tube cover 2 from the tube body 1, insert the sampled cotton swab 4 into the first cavity 11 and further pierce the isolation membrane 3, so that the double-antibody powder and the PBS glycerol mixture are mixed to obtain a buffer solution, and the buffer solution is stirred with the cotton swab 4 for multiple times to make the components in the buffer solution evenly mixed, so as to achieve the purpose of preparing and using it immediately. Then take out the cotton swab 4, tighten the tube cover 2, and place it on the tray to wait for the experimenter to perform pathological analysis. In the process of taking out the cotton swab 4, the head of the cotton swab 4 is soaked in water and swells, and is squeezed by the isolation hole 131, so that more of the sampled diseased material (sample virus) remains in the sampling tube.
[0027] Furthermore, if Figure 1-4 As shown, the tube cover 2 is provided with a sleeve hole (not shown in the figure) for fixing the cotton swab 4, and the cotton swab 4 is inserted into the sleeve hole in an interference fit manner. This arrangement is convenient for the sampler to stir the buffer solution on the one hand, and on the other hand, the sampler does not need to take out the cotton swab 5 immediately after sampling, so that the cotton swab 5 can continue to be located in the tube body 1 during transportation back to the laboratory. During the transportation vibration process, the virus sample can be more fully released into the buffer solution.
[0028] Furthermore, in order to improve the user experience, anti-slip patterns 21 are provided on the side walls of the tube cover 2 to increase the friction between the tube cover 2 and the palm.
[0029] Furthermore, in order to meet the sampling requirements, the tube body 1 is made of medical-grade PP material, and the isolation membrane 3 is made of medical-grade aluminum foil.
[0030] Furthermore, in order to extend the shelf life of the double-antibody powder and PBS glycerol mixture, the tube body 1 and the tube cover 2 are both made of brown transparent PP material.
[0031] Furthermore, if Figure 1 As shown, the bottom of the tube body 1 is conical. During the centrifugation process, the conical bottom helps to concentrate sample components such as precipitates or cells at the bottom tip, which is convenient for subsequent operations. For example, when removing the supernatant, the precipitate can be better retained to reduce sample loss.
[0032] Furthermore, the outer wall of the tube body 1 is provided with a plurality of capacity scale lines (not shown in the drawings). In the present embodiment, the capacity of the tube body 1 is 5 ml, corresponding to five capacity scale lines, namely 1.0 ml, 2.0 ml, 3.0 ml, 4.0 ml and 5.0 ml, for the convenience of the sampler in detecting the volume of the buffer solution.
[0033] The present invention also provides a swab sampling kit, which includes the aforementioned swab sampling tube containing a reagent and a cotton swab 4. The cotton swab 4 is sterile and independently packaged and sold together with the swab sampling tube containing a reagent.
[0034] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.
Claims
1. A swab sampling tube containing a reagent, characterized in that: The invention comprises a tube body for containing reagents to be mixed and used, a tube cover for preventing the reagents from leaking to the outside of the tube body is threadedly connected to the top of the tube body, a funnel part for separating the inner cavity of the tube body into a first cavity and a second cavity is sealed inside the tube body, an isolation hole for inserting a cotton swab from the first cavity into the second cavity is opened on the funnel part, and an isolation membrane is arranged on the isolation hole; the reagents include antibiotic dry powder located in the first cavity and a buffer located in the second cavity; The diameter of the isolation hole is 1-3 mm larger than the diameter of the cotton swab.
2. The swab sampling tube containing a reagent according to claim 1, characterized in that: A sleeve hole for fixing a cotton swab is arranged inside the tube cover.
3. The swab sampling tube containing a reagent according to claim 1, characterized in that: Anti-slip grooves are arranged on the side wall of the tube cover.
4. The swab sampling tube containing a reagent according to claim 1, characterized in that: The isolation film is medical grade aluminum foil.
5. The swab sampling tube containing a reagent according to claim 1, characterized in that: The tube body is made of PP material.
6. The swab sampling tube containing a reagent according to claim 5, characterized in that: Tube body brown material tube.
7. The swab sampling tube containing a reagent according to claim 1, characterized in that: The bottom of the tube is conical.
8. The swab sampling tube containing a reagent according to claim 1, characterized in that: The outer wall of the tube body is provided with a plurality of capacity scale lines.
9. A swab sampling kit, characterized in that: It comprises the swab sampling tube and cotton swab containing reagents as described in any one of claims 1 to 8.