Preparation method of traditional Chinese medicine composition for regulating lipid and dredging collaterals

Abstract

The traditional Chinese medicine composition comprises the following raw materials: fresh hawthorn, fresh ginseng, fresh gastrodia elata, fresh pseudo-ginseng, fresh radix salviae miltiorrhizae, fresh polygonum multiflorum, leech and calculus bovis, and the preparation method comprises the following steps: (1) selecting and cleaning the fresh hawthorn, fresh ginseng, fresh gastrodia elata, fresh pseudo-ginseng, fresh radix salviae miltiorrhizae and fresh polygonum multiflorum, cutting the fresh hawthorn, fresh ginseng, fresh gastrodia elata, fresh pseudo-ginseng, fresh radix salviae miltiorrhizae and fresh polygonum multiflorum into blocks, adding water to break walls, filtering, and centrifuging to obtain a supernatant; (2) carrying out ultrafiltration, concentration and freeze-drying on the supernate; (3) washing, cutting, freeze-drying, crushing and sieving the leeches; and (4) mixing the products obtained in the steps (2) and (3) with bezoar powder, performing vacuum packaging, and sterilizing to obtain the traditional Chinese medicine composition for regulating lipid and dredging collaterals, which has the effects of tonifying qi and consolidating root, promoting blood circulation to remove blood stasis, nourishing heart and dredging collaterals, is used for treating hyperlipidemia caused by kidney deficiency, liver qi stasis and blood stasis blocking collaterals and reducing complications, can effectively control blood lipid, and is simple in process, convenient to operate, safe, environment-friendly, free of toxic and side effects and suitable for clinical application. The economic and social benefits are huge.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine, and in particular to a method for preparing a lipid-regulating and blood vessel-unblocking traditional Chinese medicine composition. Background Art

[0002] Hyperlipidemia is a common clinical disease, mainly manifested as hypercholesterolemia, hypertriglyceridemia, low high-density lipoprotein levels or lipoprotein metabolism disorders. It is an important risk factor for diseases such as atherosclerosis and coronary heart disease. The typical symptoms of hyperlipidemia include xanthomas, early corneal rings, and fundus changes, but in most cases, the symptoms are not obvious and are usually discovered during visits for other diseases or routine physical examinations. In recent years, the incidence of dyslipidemia has shown a clear upward trend. In my country alone, about 35% of adults suffer from dyslipidemia to varying degrees. At present, the treatment of hyperlipidemia is mainly based on Western medicine. Although it works quickly, long-term use has great side effects, especially the impact on liver and kidney function. TCM believes that hyperlipidemia belongs to the category of "phlegm turbidity", "phlegm dampness", "blood stasis", etc., which belongs to the syndrome of deficiency of the root and excess of the superficial. Among them, the deficiency of the liver, spleen and kidney is the root, and phlegm turbidity and blood stasis are the superficial. The main reasons are improper diet and liking for fatty and sweet food, which leads to the spleen's failure to function properly and the internal generation of phlegm dampness; the kidney's failure to transform qi and the internal retention of water dampness; the liver's failure to release qi, the dysfunction of qi movement, the slow blood flow, the poor circulation of qi and blood, the turbidity of body fluids, the generation of phlegm, and the blocking of the meridians. The turbidity of phlegm leads to qi stagnation and blood stasis for a long time, and the blood stasis affects the metabolism of water. The accumulation of water dampness generates phlegm turbidity, and the phlegm and blood stasis are mutually bound, which stays in the meridians and blocks the meridians to form hyperlipidemia. TCM starts from the overall perspective, differentiates and treats based on syndrome differentiation, and regulates the balance of yin and yang of the internal organs. It has unique advantages in the treatment of hyperlipidemia. Therefore, it is necessary to develop a new, efficient and stable Chinese medicine for lipid regulation and meridian unblocking.

[0003] Fresh Chinese medicine is an important feature of traditional Chinese medicine. It has unique efficacy in the clinical treatment of acute and critical diseases and difficult and complicated diseases, and its efficacy is better than that of dry products. So far, there has been no public report on whether fresh Chinese medicine can be used to develop a Chinese medicine composition for lipid regulation and blood circulation. Summary of the invention

[0004] In view of the above situation, in order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition, which can effectively solve the problem of medication for controlling blood lipids.

[0005] The technical solution provided by the present invention is a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition, wherein the Chinese medicine composition is composed of the following raw materials in parts by weight: 10 to 60 parts of fresh hawthorn, 10 to 50 parts of fresh ginseng, 10 to 60 parts of fresh gastrodia, 10 to 60 parts of fresh notoginseng, 10 to 60 parts of fresh salvia, 10 to 60 parts of fresh polygonum multiflorum, 0.2 to 3 parts of leech and 0.05 to 0.5 parts of bezoar, and the preparation method is:

[0006] (1) The fresh hawthorn is selected and washed 1 to 3 times, cut into pieces or slices while fresh, and water is added in an amount of 0.5 to 4 times the weight volume of the original drug, where the weight volume refers to the solid in kg and the liquid in L (the same below), and the cell wall is broken for 1 to 20 minutes, filtered, and the filtrate is centrifuged at 2000 to 8000 r / min for 5 to 30 minutes to obtain the first supernatant; then water is added and the cell wall is broken and centrifuged 1 to 4 times according to the above method to obtain the second to fifth supernatants, and the supernatants of each time are combined;

[0007] After selection, fresh Panax notoginseng is washed 1 to 3 times, cut into sections or slices while fresh, add water 1 to 5 times the weight and volume of the original drug, break the cell wall for 1 to 30 minutes, filter, and centrifuge the filtrate at 2000 to 8000 r / min for 5 to 30 minutes to obtain the first supernatant; then add water and repeat the cell wall breaking and centrifugation 1 to 4 times according to the above method to obtain the second to fifth supernatants, and combine the supernatants of each time;

[0008] Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant;

[0009] (2) ultrafiltration of each supernatant obtained in the above (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating it using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing it to obtain each freeze-dried powder;

[0010] (3) Wash and cut the leech into segments, freeze-dry, grind into coarse powder, pass through an 80-100 mesh sieve, and then ultrafine grind and pass through a 150-300 mesh sieve to obtain leech ultrafine powder;

[0011] (4) uniformly mixing the freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3), and the crushed bezoar powder, vacuum-packing, and sterilizing to obtain a lipid-regulating and meridian-opening Chinese medicine composition, which is stored at -20°C for future use; the sterilization method includes but is not limited to ultra-high pressure sterilization, pasteurization, radiation sterilization, gas sterilization, filtration sterilization, etc.

[0012] The application of the lipid-regulating and meridian-unblocking Chinese medicine composition in the preparation of lipid-regulating and meridian-unblocking medicines.

[0013] The lipid-regulating and meridian-unblocking Chinese medicine composition is further added with pharmaceutically acceptable excipients to prepare lipid-regulating and meridian-unblocking medicines in different Chinese medicine dosage forms.

[0014] The traditional Chinese medicine dosage forms are granules, tablets and capsules.

[0015] The lipid-regulating and blood-clearing Chinese medicine composition is used in preparing drugs for preventing, alleviating and treating dyslipidemia.

[0016] The Chinese medicine composition of the present invention plays a synergistic role according to the primary and secondary relationship of monarch, minister, assistant and envoy, has the effects of invigorating qi and strengthening the foundation, promoting blood circulation and removing blood stasis, nourishing the heart and dredging the collaterals, is used to treat hyperlipidemia caused by kidney deficiency, liver qi stagnation and blood stasis blocking the collaterals, reduces complications, and long-term use of the Chinese medicine composition of the present invention can effectively control blood lipids and keep them within a normal range. At the same time, the Chinese medicine composition with lipid-regulating and blood vessel-dredging effects and the preparation method of the present invention have simple process, convenient operation, safety and environmental protection, can retain the active ingredients in the Chinese medicine to the greatest extent, ensure the efficacy of the medicine, have no toxic side effects when taken for a long time, have good practical clinical application value and development and application prospects, and have huge economic and social benefits. DETAILED DESCRIPTION

[0017] The specific implementation modes of the present invention are described in detail below in combination with embodiments and specific situations.

[0018] Example 1

[0019] The invention discloses a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition. The Chinese medicine composition is composed of the following raw materials in parts by weight: 35 parts of fresh hawthorn, 30 parts of fresh ginseng, 35 parts of fresh gastrodia, 35 parts of fresh notoginseng, 35 parts of fresh salvia, 35 parts of fresh polygonum multiflorum, 1.6 parts of leech and 0.3 part of bezoar. The preparation method is:

[0020] (1) The fresh hawthorn is selected and washed twice, cut into 2-4 mm thick slices while fresh, and added with 1 times the weight volume of water of the original drug, broken for 5 minutes, filtered, and the filtrate is centrifuged at 4000r / min for 20 minutes to obtain the first supernatant; then 3 times the amount of water is added and the wall is broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time are combined;

[0021] Fresh Panax notoginseng was selected and washed twice, cut into 2-4 mm thick slices while fresh, added with water twice the weight of the original drug, broken for 8 minutes, filtered, and the filtrate was centrifuged at 4000r / min for 20 minutes to obtain the first supernatant; then 4 times the amount of water was added and the wall was broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time were combined;

[0022] Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant;

[0023] (2) ultrafiltration of each supernatant obtained in the above (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating it using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing it to obtain each freeze-dried powder;

[0024] (3) Washing and cutting the leech medicinal material, freeze-drying, first grinding into coarse powder, passing through a 100-mesh sieve, and then ultrafine grinding, passing through a 300-mesh sieve, to obtain leech ultrafine powder;

[0025] (4) uniformly mixing the freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3), and the crushed bezoar powder, vacuum-packing, and sterilizing to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

[0026] Example 2

[0027] The invention discloses a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition. The Chinese medicine composition is composed of the following raw materials in parts by weight: 60 parts of fresh hawthorn, 50 parts of fresh ginseng, 60 parts of fresh gastrodia, 60 parts of fresh notoginseng, 60 parts of fresh salvia, 60 parts of fresh polygonum multiflorum, 3 parts of leech and 0.5 part of bezoar. The preparation method is:

[0028] (1) washing the selected cells twice, cutting them into 2-4 mm thick slices while they are still fresh, adding water 3 times the weight volume of the original drug, breaking the cell wall for 10 minutes, filtering, and centrifuging the filtrate at 6000 r / min for 15 minutes to obtain the first supernatant; then adding water and repeating the above method to break the cell wall and centrifuge once to obtain the second supernatant, and combining the supernatants of each time;

[0029] Fresh Panax notoginseng was selected and washed twice, and cut into 2-4 mm thick slices while fresh, and water 4 times the weight and volume of the original drug was added to break the cell wall for 10 minutes, filtered, and the filtrate was centrifuged at 6000r / min for 15 minutes to obtain the first supernatant; then water was added and the cell wall was broken and centrifuged again according to the above method to obtain the second supernatant, and the supernatants of each time were combined;

[0030] Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant;

[0031] (2) ultrafiltration of each supernatant obtained in the above (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating it using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing it to obtain each freeze-dried powder;

[0032] (3) Washing and cutting the leech medicinal material, freeze-drying, first grinding it into coarse powder, passing it through a 100-mesh sieve, and then ultrafine grinding it, passing it through a 220-mesh sieve, to obtain leech ultrafine powder;

[0033] (4) uniformly mixing the freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3), and the crushed bezoar powder, vacuum-packing, and sterilizing to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

[0034] Example 3

[0035] The invention discloses a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition. The Chinese medicine composition is composed of the following raw materials in parts by weight: 10 parts of fresh hawthorn, 10 parts of fresh ginseng, 10 parts of fresh gastrodia elata, 10 parts of fresh notoginseng, 10 parts of fresh salvia miltiorrhiza, 10 parts of fresh polygonum multiflorum, 0.2 parts of leech and 0.05 parts of bezoar. The preparation method is:

[0036] (1) The fresh hawthorn is selected and washed once, cut into 2-4 mm thick slices while fresh, and water 4 times the weight volume of the original drug is added, the wall is broken for 20 minutes, filtered, and the filtrate is centrifuged at 6000 r / min for 30 minutes to obtain a supernatant;

[0037] Fresh Panax notoginseng was selected and washed once, cut into 2-4 mm thick slices while fresh, added with water 5 times the weight and volume of the original drug, broken for 20 minutes, filtered, and the filtrate was centrifuged at 6000r / min for 30 minutes to obtain the supernatant;

[0038] Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant;

[0039] (2) ultrafiltration of each supernatant obtained in the above (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating it using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing it to obtain each freeze-dried powder;

[0040] (3) Washing and cutting the leech medicinal material, freeze-drying, first grinding it into coarse powder, passing it through an 80-mesh sieve, and then ultrafine grinding it, passing it through a 150-mesh sieve, to obtain leech ultrafine powder;

[0041] (4) uniformly mixing the freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3), and the crushed bezoar powder, vacuum-packing, and sterilizing to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

[0042] Example 4

[0043] The invention discloses a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition. The Chinese medicine composition is composed of the following raw materials in parts by weight: 50 parts of fresh hawthorn, 20 parts of fresh ginseng, 50 parts of fresh gastrodia, 20 parts of fresh notoginseng, 50 parts of fresh salvia, 20 parts of fresh Polygonum multiflorum, 2.3 parts of leech and 0.4 part of bezoar. The preparation method is:

[0044] (1) The fresh hawthorn is selected and washed for 1 to 3 times, cut into sections or slices while fresh, and water of 0.5 times the weight volume of the original drug is added, the cell wall is broken for 10 minutes, filtered, and the filtrate is centrifuged at 2000 r / min for 30 minutes to obtain a first supernatant; then water of 3 times the weight volume of the original drug is added, the cell wall is broken for 5 minutes, filtered, and the filtrate is centrifuged at 5000 r / min for 15 minutes to obtain a second supernatant, and water is added according to the above method and the cell wall is broken and centrifuged twice to obtain the third to fourth supernatants, and the supernatants of each time are combined;

[0045] After selection, fresh Panax notoginseng is washed 1 to 3 times, cut into sections or slices while fresh, add water with a volume of 1 times the weight of the original drug, break the cell wall for 10 minutes, filter, and centrifuge the filtrate at 2000r / min for 30 minutes to obtain the first supernatant; then add water with a volume of 3 times the weight of the original drug, break the cell wall for 5 minutes, filter, and centrifuge the filtrate at 5000r / min for 15 minutes to obtain the second supernatant, and then add water according to the above method and repeat the cell wall breaking and centrifugation for 2 times to obtain the 3rd to 4th supernatant, and combine the supernatants of each time;

[0046] Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant;

[0047] (2) ultrafiltration of each supernatant obtained in the above (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating it using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing it to obtain each freeze-dried powder;

[0048] (3) Washing and cutting the leech medicinal material, freeze-drying, first grinding it into coarse powder, passing it through a 100-mesh sieve, and then ultrafine grinding it, passing it through a 280-mesh sieve, to obtain ultrafine leech powder;

[0049] (4) uniformly mixing the freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3), and the crushed bezoar powder, vacuum-packing, and sterilizing to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

[0050] Example 5

[0051] The invention discloses a method for preparing a lipid-regulating and blood-clearing Chinese medicine composition. The Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh hawthorn, 40 parts of fresh ginseng, 20 parts of fresh gastrodia, 50 parts of fresh notoginseng, 20 parts of fresh salvia, 50 parts of fresh Polygonum multiflorum, 0.9 parts of leech and 0.2 parts of bezoar. The preparation method is:

[0052] (1) The fresh hawthorns are selected and washed three times, cut into segments while fresh, and added with water twice the weight volume of the original drug, broken for 8 minutes, filtered, and the filtrate is centrifuged at 8000 r / min for 5 minutes to obtain the first supernatant; then water is added and the cell wall is broken and centrifuged four times according to the above method to obtain the second to fifth supernatants, and the supernatants of each time are combined;

[0053] Fresh Panax notoginseng was selected and washed three times, cut into segments while fresh, added with water twice the weight and volume of the original drug, broken for 10 minutes, filtered, and the filtrate was centrifuged at 8000r / min for 5 minutes to obtain the first supernatant; then water was added and the cell wall was broken and centrifuged four times according to the above method to obtain the second to fifth supernatants, and the supernatants of each time were combined;

[0054] Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant;

[0055] (2) ultrafiltration of each supernatant obtained in the above (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating it using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing it to obtain each freeze-dried powder;

[0056] (3) Washing and cutting the leech medicinal material, freeze-drying, first grinding into coarse powder, passing through an 80-mesh sieve, and then ultrafine grinding, passing through a 180-mesh sieve, to obtain leech ultrafine powder;

[0057] (4) uniformly mixing the freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3), and the crushed bezoar powder, vacuum-packing, and sterilizing to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

[0058] The application of the lipid-regulating and meridian-unblocking Chinese medicine composition in the preparation of lipid-regulating and meridian-unblocking medicines.

[0059] The lipid-regulating and meridian-unblocking Chinese medicine composition is further added with pharmaceutically acceptable excipients to prepare lipid-regulating and meridian-unblocking medicines in different Chinese medicine dosage forms.

[0060] The traditional Chinese medicine dosage forms are granules, tablets and capsules.

[0061] The lipid-regulating and blood-clearing Chinese medicine composition is used in preparing drugs for preventing, alleviating and treating dyslipidemia.

[0062] In the above-mentioned Chinese medicine composition, wherein:

[0063] Fresh hawthorn: sour, sweet, slightly warm in nature. Enters the spleen, stomach, and liver meridians. Helps digestion and invigorates the stomach, promotes qi and dissipates blood stasis, clears turbidity and reduces fat.

[0064] Fresh ginseng: sweet, slightly bitter, slightly warm in nature. Enters the spleen, lung, heart, and kidney meridians. It greatly replenishes vital energy, replenishes qi and produces body fluids, calms the mind and improves intelligence.

[0065] Fresh Panax notoginseng: sweet, slightly bitter, warm in nature. Enters the liver and stomach meridians. Disperses blood stasis, stops bleeding, reduces swelling and relieves pain.

[0066] Fresh Gastrodia elata: sweet in taste, neutral in nature. Enters the liver meridian. Calms wind and stops spasms, calms liver yang, dispels wind and dredges meridians.

[0067] Fresh Salvia miltiorrhiza: bitter, slightly cold in nature. Enters the heart, liver, and spleen meridians. It activates blood circulation, removes blood stasis, cools blood, eliminates carbuncle, clears the heart and relieves restlessness.

[0068] Fresh Polygonum multiflorum: bitter, sweet, astringent, slightly warm. Enters the liver, heart, and kidney meridians. Replenishes essence and blood, detoxifies, eliminates carbuncle, stops malaria, and moistens the intestines and promotes bowel movements.

[0069] Leech: salty, bitter, neutral in nature; slightly toxic. Enters the liver meridian. Breaks blood, dredges the meridians, removes blood stasis and resolves symptoms.

[0070] Bezoar: sweet in taste, cool in nature. Enters the heart and liver meridians. Clears the heart, dispels phlegm, opens the orifices, cools the liver, calms the wind, and detoxifies.

[0071] The above components are scientifically and reasonably combined according to the primary and secondary compatibility principle of traditional Chinese medicine monarch, minister, assistant and envoy, and work synergistically with each other. Fresh ginseng has the effects of greatly replenishing vital energy, invigorating qi and promoting body fluid, calming the mind and improving intelligence, can promote blood circulation, remove blood stasis and dredge the meridians. Fresh Panax notoginseng is a commonly used medicine for promoting blood circulation and removing blood stasis, has the effects of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, can replenish qi and blood, and has the function of promoting blood circulation without hurting blood. The two are the monarch medicines together, and play the effects of replenishing qi and dredging the meridians, nourishing blood and activating blood circulation; fresh Salvia miltiorrhiza can remove blood stasis and activate blood circulation, promote menstruation and relieve pain. A single Salvia miltiorrhiza drink is better than Siwu Decoction, and can clear the heart and relieve troubles. Using it together with fresh ginseng can clear the heart and nourish blood. Fresh Gastrodia elata has the effects of calming the liver and calming wind, dredging the meridians and relieving pain, and can assist fresh Panax notoginseng in strengthening the effect of dredging the meridians and relieving pain. Fresh Polygonum multiflorum has the effects of nourishing the liver and kidney, and benefiting the essence and blood. , strengthening muscles and bones, can assist in strengthening the overall physique and promote recovery. Fresh hawthorn has the effects of digesting food and removing accumulation, promoting qi and dispersing blood stasis. When used together with fresh ginseng, it can enhance the effect of promoting blood circulation and removing blood stasis, while promoting the functioning of the spleen and stomach, and better play the role of nourishing qi and blood. The four medicines are together the ministers, which can significantly enhance the effects of invigorating qi and unblocking collaterals, promoting blood circulation and removing blood stasis, and removing blood stasis without hurting the body; leeches have the effects of breaking blood and dredging menstruation, removing blood stasis and eliminating symptoms. Its blood-breaking power is relatively strong, and it can assist the monarch and ministers in enhancing the effect of promoting blood circulation and removing blood stasis. Bezoar has the effects of clearing the heart and resolving phlegm, opening the orifices and refreshing the mind, cooling the liver and calming the wind, clearing away heat and detoxifying. When used together with fresh salvia miltiorrhiza, it can enhance the effects of clearing the heart and removing troubles, promoting blood circulation and unblocking collaterals. Leeches and bezoar are used together as adjuvants to enhance the effects of removing blood stasis and unblocking collaterals, clearing the heart and nourishing blood. The combination of various medicines can replenish qi and strengthen the body, promote blood circulation and remove blood stasis, nourish the heart and dredge the collaterals, and can be effectively used to treat hyperlipidemia caused by kidney deficiency, liver qi stagnation, and blood stasis blocking the collaterals, reduce complications, and achieve very good beneficial technical effects. The relevant test data are as follows (taking Example 1 as an example):

[0072] 1. Clinical case selection

[0073] A total of 50 cases were selected from outpatient clinics, including 25 males and 25 females with an average age of 48.9 years.

[0074] 1.1. Western medicine diagnostic criteria

[0075] According to the 2016 "Guidelines for the Prevention and Treatment of Dyslipidemia in Chinese Adults", dyslipidemia can be diagnosed when ≥ 1 of the following fasting venous plasma test indicators is met:

[0076] ①Total cholesterol (TC) ≥ 6.2mmol / L;

[0077] ② Low-density lipoprotein cholesterol (LDL-C) ≥ 4.1mmol / L;

[0078] ③Triglyceride (TG) ≥ 2.3mmol / L;

[0079] ④ When high-density lipoprotein cholesterol (HDL-C) is less than 1.0mmol / L;

[0080] ⑤TC ≥ 5.2mmol / L and LDL-C ≥ 3.4mmol / L are defined as marginal elevation, which aims to remind patients to strengthen blood lipid testing.

[0081] 1.2. TCM diagnostic criteria

[0082] According to the standards of "Classification and Determination of TCM Constitution" published by the China Association of Traditional Chinese Medicine in 2009, patients are identified as having both Qi deficiency and blood stasis constitution.

[0083] Main symptoms: fatigue; loss of appetite; loose stools; stabbing pain in the precordial area; forgetfulness. (None (0 points), mild (2 points), moderate (4 points), severe (6 points))

[0084] Secondary symptoms: numbness and heaviness in the limbs; distension in the abdomen; dizziness; pain in the body; tastelessness in the mouth. (None (0 points), mild (1 point), moderate (2 points), severe (3 points)).

[0085] Tongue and pulse: The tongue is pale and dark, with white fur, petechiae or ecchymosis can be seen on the tongue, and the pulse is sluggish and weak.

[0086] Patients who meet at least 2 of the above main symptoms (including fatigue) and at least 2 of the secondary symptoms can be diagnosed.

[0087] 1.3 Inclusion criteria

[0088] ① Meet the diagnostic criteria for hyperlipidemia in the "Guidelines for the Prevention and Treatment of Dyslipidemia in Chinese Adults" and "Practical Internal Medicine";

[0089] ② Comply with the description of the symptoms of Qi deficiency and blood stasis in the "Guidelines for Clinical Research of New Chinese Medicines" and "Diagnostics of Traditional Chinese Medicine";

[0090] ③ Aged between 18 and 70 years old, and have signed the informed consent.

[0091] 1.4 Exclusion criteria

[0092] ① Those who do not meet the above-mentioned Chinese and Western medicine diagnostic criteria;

[0093] ② Those with severe diabetes who need insulin control;

[0094] ③ Hyperlipidemia caused by nephrotic syndrome, hypothyroidism, gout, acute and chronic liver and gallbladder diseases, etc.;

[0095] ④ Patients with hyperlipidemia and homozygous hypercholesterolemia caused by drugs (β-blockers, adrenal corticosteroids and certain contraceptives, etc.);

[0096] ⑤ Patients with severe primary diseases of the liver, kidney and hematopoietic system, and mental illness;

[0097] ⑥ Patients with severe heart failure and acute cerebrovascular accident;

[0098] ⑦ Patients with various acute and chronic infections.

[0099] 1.5. Falling off and rejection criteria

[0100] ① Those who failed to complete the clinical observation for some reason;

[0101] ② Those who have serious adverse events during the observation and treatment process and are not suitable to continue the trial;

[0102] ③ Actively request to withdraw from clinical observation.

[0103] 2. TCM syndrome efficacy evaluation criteria

[0104] Refer to the standards of "Clinical Guidance for New Chinese Medicines".

[0105] Markedly effective: All fasting indices basically returned to normal, clinical symptoms and signs were significantly improved, and the symptom score decreased by ≥70% and <95%; blood lipid improvement reached any one of the following: TC (total cholesterol) decreased by ≥20%, TG (triglyceride) decreased by ≥40%, and HDL-C increased by ≥0.26mmol / L.

[0106] Effective: clinical symptoms and signs are improved, with symptom score ≥30% and <70%; blood lipids improve to any of the following: TC decreases ≥10% but <20%, TG decreases >20% but <40%, HDL-C increases ≥0.13mmol / L but <0.26mmol / L;

[0107] Ineffective: There is no improvement in clinical symptoms and signs, and there is even a tendency to worsen, and the symptom score decreases by less than 30%.

[0108] The nimodipine method was used for calculation: therapeutic index N = [(pre-treatment score - post-treatment score) / pre-treatment score] × 100%;

[0109] The levels of blood lipids TC and TG were checked once in the second and fourth weeks respectively;

[0110] 3. Treatment options

[0111] A total of 50 confirmed patients were randomly divided into two groups (experimental group and control group), with 25 people in each group.

[0112] Experimental group: the Chinese medicine composition of the present invention was taken orally, twice a day, 2 g (4 capsules) each time.

[0113] Control group: rosuvastatin, oral, dose of 5 mg, once a day.

[0114] One course of treatment was one month, and the efficacy of the two groups was statistically analyzed after one course of treatment.

[0115] 4. Statistical processing

[0116] 4.1. General Data Comparison

[0117] 4.1.1 Age distribution of subjects

[0118] According to the treatment scheme of the present invention, 50 outpatients diagnosed with the disease were randomly divided into a treatment group and a control group with 25 cases in a ratio of 1:1 using a random number table method: the treatment group had 18 females and 17 males, aged 18 to 69 years old, with a course of 6 to 24 months; the control group had 17 females and 18 males, aged 19 to 70 years old, with a course of 7 to 25 months. There was no significant difference in gender, age, course of disease and other general data between the two groups after statistical analysis (P>0.05), and they were comparable.

[0119] 4.1.2 Effectiveness after taking

[0120] Note: The efficacy index N = [(pre-treatment score - post-treatment score) / pre-treatment score] × 100%, divided into:

[0121] Markedly effective: clinical symptoms and signs are significantly improved, with symptom scores reduced by ≥70% and <95%;

[0122] Effective: clinical symptoms and signs have improved, with symptom score ≥30% and <70%;

[0123] Ineffective: clinical symptoms and signs have no significant improvement, or even worsen, and the symptom score decreases by less than 30%;

[0124] Comparison of the scores of the main symptoms of kidney yang deficiency before and after treatment in the present invention group

[0125] 4.1.2.1 Effectiveness of the two groups of patients after taking the medicine for 2 weeks

[0126] According to the criteria for determining efficacy in the "Clinical Guidelines for New Traditional Chinese Medicines", the total effective rates of the two groups of patients after taking the medicine for 2 weeks were 66% and 26%, respectively, as shown in Table 1.

[0127] 4.1.2.2 Effectiveness of the two groups of patients after taking the medicine for 4 weeks

[0128] According to the criteria for determining efficacy in the "Clinical Guidelines for New Traditional Chinese Medicines", the total effective rates of the two groups of patients after taking the medicine for 4 weeks were 100% and 80%, respectively, as shown in Table 2.

[0129] 4.1.3 Comparison of scores of main symptoms of Qi deficiency and blood stasis before and after treatment

[0130] After taking the medicine of the present invention, the scores of the three main symptoms of kidney yang deficiency of the subjects were significantly reduced, and the difference was statistically significant, as shown in Table 3.

[0131] 4.1.4 Comparison of scores of Qi deficiency and blood stasis before and after treatment

[0132] The scores of various symptoms before and after taking the drug of the present invention were reduced to varying degrees. According to statistical analysis, the scores of mental fatigue, frequent urination at night, poor sleep, and shortness of breath when moving were statistically significant compared with the scores before treatment (P<0.05), as shown in Table 4.

[0133] 4.1.5 Changes in blood lipids before and after treatment

[0134] There were statistically significant differences in the various blood lipid indicators before and after treatment, as shown in Table 5.

[0135] 4.1.6 Effects of regulating lipids and dredge meridians

[0136] The experimental results show that the total effective rate of reducing blood lipids in the experimental group (Chinese medicine composition of the present invention) is 100%, and the total effective rate of reducing blood lipids in the control group is 62%, as shown in Table 6. At the same time, it can be seen from the experimental results of Tables 5 and 6 that the blood lipid levels of patients taking the present invention are lower than those of patients taking the control group, and are within the normal range. This shows that the pharmaceutical composition of the present invention has a good effect of reducing blood lipids, and the therapeutic effect is significantly better than that of the control group.

[0137] 4.1.7 Data Analysis Methods

[0138] All data were statistically analyzed using SPSS 22.0. Normally distributed data were expressed as mean ± standard deviation. The data of the grade were expressed as median (interquartile range). The t test was used to compare the two groups of normal distribution data; the Wilcoxon rank sum test was used to compare the two groups of graded data. P < 0.05 was considered statistically significant.

[0139] Table 1 Effectiveness of subjects after 2 weeks of treatment

[0140]

[0141] Table 2 Effectiveness of subjects after taking for 4 weeks

[0142]

[0143] Table 3 Comparison of scores of the main symptoms of Qi deficiency and blood stasis in the group of the present invention before and after treatment (n=50, )

[0144]

[0145] Note: *P<0.05 compared with before treatment

[0146] Table 4 Comparison of scores of Qi deficiency and blood stasis in the present invention group before and after treatment (n=50, )

[0147]

[0148] Note: *P<0.05 compared with before treatment

[0149] Table 5 Changes of blood lipids in the subjects of the present invention group and the control group before and after treatment (n=50, )

[0150]

[0151] Note: P<0.05 compared with before treatment

[0152] Table 6 Experimental results of lipid-regulating and blood-clearing in each group

[0153]

[0154] 5. Typical Cases

[0155] Case 1: Zhang, female, 49 years old, from XX City, Henan Province. Hyperlipidemia patients, before taking the present invention, had soreness of waist and knees, fear of cold and cold limbs accompanied by mental weakness, lower limb edema, pale tongue with white fur, slow pulse, weak wrist and other symptoms. TC in the blood was 7.2 mmol / L, TG was 4.2 mmol / L; after taking the present invention for one course of treatment, soreness of waist and knees, fear of cold and cold limbs and complications were significantly improved, TC in the blood was 5.8 mmol / L, TG was 1.1 mmol / L, and blood lipids were maintained within the normal range after continuous use.

[0156] Case 2: Wang, male, 63 years old, from XX City, Henan Province. Hyperlipidemia patient, before taking the present invention, had soreness of waist and knees, loss of libido, accompanied by mental weakness, frequent urination at night, shortness of breath when moving, pale tongue with white fur. TC 8.4 mmol / L, TG 3.8 mmol / L in blood; after taking the present invention for one course of treatment, soreness of waist and knees, loss of libido and complications were significantly improved, TC 5.3 mmol / L, TG 1.4 mmol / L in blood, continuous use, blood lipids maintained in the normal range.

[0157] Case 3: Li, male, 38 years old, from XX City, Henan Province. Patients with hyperlipidemia, before taking the present invention, had soreness of waist and knees, loss of libido, fear of cold and cold limbs. Minor symptoms: mental weakness, frequent urination at night, edema of lower limbs, shortness of breath when moving, dry hair and loose teeth, pale tongue with white fur, slow pulse, weak pulse. TC 9.1 mmol / L, TG 4.7 mmol / L in blood; after taking the present invention for one course of treatment, soreness of waist and knees, loss of libido, fear of cold and cold limbs and complications were significantly improved. TC 4.8 mmol / L, TG 1.7 mmol / L in blood, continuous use, blood lipids are maintained within the normal range.

[0158] Case 4: Zhu, female, 47 years old, from XXX City, Henan Province. Hyperlipidemia patients, before taking the present invention, had decreased libido, fear of cold and cold limbs accompanied by mental weakness, dry hair and loose teeth, pale tongue with white fur, slow pulse, and weak pulse. TC 7.8 mmol / L, TG 5.1 mmol / L in blood; after taking the present invention for one course of treatment, decreased libido, fear of cold and cold limbs and complications were significantly improved. TC 5.0 mmol / L, TG 0.9 mmol / L in blood, continuous use, blood lipids maintained in the normal range.

[0159] Case 5: Han, male, 35 years old, from XXX City, Henan Province. Hyperlipidemia patient, before taking the present invention, had soreness of waist and knees, loss of libido, accompanied by mental weakness, frequent urination at night, lower limb edema, slow pulse, weak wrist. TC in blood 8.3 mmol / L, TG 4.8 mmol / L; after taking the present invention for one course of treatment, soreness of waist and knees, loss of libido and complications were significantly improved. TC in blood 4.9 mmol / L, TG 1.6 mmol / L, continuous use, blood lipids maintained in the normal range.

[0160] Case 6: Xu, male, 30 years old, from XX City, Henan Province. Hyperlipidemia patients, before taking the present invention, had soreness of waist and knees, loss of libido, fear of cold and cold limbs, and frequent urination at night, lower limb edema, pale tongue with white fur. TC 10.1 mmol / L, TG 4.0 mmol / L in blood; after taking the present invention for one course of treatment, soreness of waist and knees, loss of libido, fear of cold and cold limbs and complications were significantly improved. TC 4.5 mmol / L, TG 0.7 mmol / L in blood, continuous use, blood lipids maintained in the normal range.

[0161] Case 7: Shang Moumou, female, 51 years old, from Zhumadian, Henan. Patients with hyperlipidemia, before taking the present invention, had soreness of waist and knees, fear of cold and cold limbs, accompanied by mental weakness, lower limb edema, shortness of breath when moving, dry hair and loose teeth, pale tongue with white fur, slow pulse, and weak foot. TC in the blood was 9.4 mmol / L, TG was 6.2 mmol / L; after taking the present invention for a course of treatment, soreness of waist and knees, fear of cold and cold limbs and complications were significantly improved. TC in the blood was 5.3 mmol / L, TG was 1.1 mmol / L, and blood lipids were maintained within the normal range after continuous use.

[0162] Case 8: Ren, male, 38 years old, from XX City, Henan Province. Hyperlipidemia patients, before taking the present invention, had soreness of waist and knees, loss of libido, accompanied by mental weakness, frequent urination at night, dry hair and loose teeth, pale tongue with white fur, slow pulse, weak pulse (. TC7.4 mmol / L, TG3.6 mmol / L in blood; after taking the present invention for one course of treatment, soreness of waist and knees, loss of libido and complications were significantly improved. TC5.8 mmol / L, TG1.9 mmol / L in blood, continuous use, blood lipids maintained in the normal range.

[0163] Case 9: Sun, female, 60 years old, from XX City, Henan Province. Hyperlipidemia patients, before taking the present invention, had soreness of waist and knees, fear of cold and cold limbs, accompanied by mental weakness, frequent urination at night, edema of lower limbs, shortness of breath when moving, dry hair and loose teeth, pale tongue with white fur, slow pulse, weak pulse (. TC 6.9 mmol / L, TG 5.0 mmol / L in blood; after taking the present invention for one course of treatment, soreness of waist and knees, fear of cold and cold limbs and complications were significantly improved. TC 4.1 mmol / L, TG 2.0 mmol / L in blood, continuous use, blood lipids are maintained within the normal range.

[0164] Case 10: Zhao Moumou, male, 42 years old, from XX City, Henan Province. Hyperlipidemia patients, before taking the present invention, had soreness of waist and knees, loss of libido, fear of cold and cold limbs, accompanied by mental weakness, frequent urination at night, edema of lower limbs, pale tongue with white fur, slow pulse, and weak pulse. TC 11.2 mmol / L, TG 4.7 mmol / L in blood; after taking the present invention for one course of treatment, soreness of waist and knees, loss of libido, fear of cold and cold limbs and complications were significantly improved. TC 4.4 mmol / L, TG 1.5 mmol / L in blood, continuous use, blood lipids are maintained in the normal range.

[0165] While conducting experiments on Example 1, the same experiments were also conducted on other examples, and the same or similar results were obtained, which will not be listed here one by one.

[0166] 5. Conclusion

[0167] In summary, the pharmaceutical composition of the present invention is compatible according to the primary and secondary relationship of monarch, minister, assistant and envoy, and the prescription is scientific and reasonable. Fresh ginseng and fresh Panax notoginseng can invigorate qi and dredge collaterals, nourish blood and promote blood circulation. Fresh salvia miltiorrhiza, fresh gastrodia elata, fresh Polygonum multiflorum and fresh hawthorn can significantly enhance the effects of invigorating qi and dredge collaterals, promoting blood circulation and removing blood stasis, and remove blood stasis without hurting the body. Leech and bezoar are used as adjuvants to enhance the effects of removing blood stasis and dredge collaterals, clearing the heart and nourishing blood. The combination of all the medicines can invigorate qi and consolidate the foundation, promote blood circulation and remove blood stasis, nourish the heart and dredge collaterals, and can be effectively used to treat hyperlipidemia caused by kidney deficiency, liver qi stagnation and blood stasis blocking the collaterals. At the same time, the Chinese medicine composition of the present invention forms a stable fresh medicine preparation process. After wall breaking and pulverization, the effective ingredients of each raw material medicine can be fully released, making full use of them, saving medicinal material resources, having the effect of lowering blood lipids, significantly improving the therapeutic effect, with an effective rate of up to 100%, no toxic side effects, and being able to keep blood lipids within a normal range. Moreover, the Chinese medicine composition can be prepared into pharmaceutically permitted dosage forms such as granules, tablets and capsules according to actual needs, has strong practical application value, is conducive to industrial production and development, promotion and application, and has huge economic and social benefits.

Claims

1. A method for preparing a lipid-regulating and blood-clearing Chinese medicine composition, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 10-60 parts of fresh hawthorn, 10-50 parts of fresh ginseng, 10-60 parts of fresh gastrodia, 10-60 parts of fresh notoginseng, 10-60 parts of fresh salvia, 10-60 parts of fresh polygonum multiflorum, 0.2-3 parts of leech and 0.05-0.5 parts of bezoar. The preparation method is: (1) After selecting the fresh hawthorn, wash it 1 to 3 times, cut it into pieces or slices while it is fresh, add water 0.5 to 4 times the weight volume of the original drug, the weight volume refers to the solid in kg and the liquid in L, break the wall for 1 to 20 minutes, filter, and centrifuge the filtrate at 2000 to 8000 r / min for 5 to 30 minutes to obtain the first supernatant; then add water and repeat the above method of breaking the wall and centrifuging 1 to 4 times to obtain the second to fifth supernatants, and combine the supernatants of each time; After selection, fresh Panax notoginseng is washed 1-3 times, cut into sections or slices while fresh, add 1-5 times the weight and volume of water of the original drug, break the wall for 1-30 minutes, filter, and centrifuge the filtrate at 2000-8000 r / min for 5-30 minutes to obtain the first supernatant; then add water and repeat the above method of breaking the wall and centrifuging 1-4 times to obtain the second to fifth supernatants, and combine the supernatants of each time; Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant; (2) The supernatants obtained in the above (1) are respectively ultrafiltered using a 10000D ultrafiltration membrane to remove macromolecular substances, and the ultrafiltration permeates are collected and then concentrated using a 200D nanofiltration membrane to obtain nanofiltration concentrates; then the nanofiltration concentrates are freeze-dried, and the freeze-dried products are collected and pulverized to obtain freeze-dried powders; (3) Wash and cut the leech into segments, freeze-dry, grind into coarse powder, pass through an 80-100 mesh sieve, and then ultrafine grind and pass through a 150-300 mesh sieve to obtain leech ultrafine powder; (4) The freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3) and the crushed bezoar powder are uniformly mixed, vacuum-packed and sterilized to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

2. The method for preparing the lipid-regulating and blood-clearing Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 35 parts of fresh hawthorn, 30 parts of fresh ginseng, 35 parts of fresh gastrodia, 35 parts of fresh notoginseng, 35 parts of fresh salvia, 35 parts of fresh polygonum multiflorum, 1.6 parts of leech and 0.3 parts of bezoar. The preparation method is: (1) The fresh hawthorns are selected and washed twice, and sliced ​​into 2-4 mm thick slices while fresh, and water of 1 times the weight volume of the original drug is added to break the cell wall for 5 minutes, filtered, and the filtrate is centrifuged at 4000 r / min for 20 minutes to obtain the first supernatant; then 3 times the amount of water is added and the cell wall is broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time are combined; Fresh Panax notoginseng was selected and washed twice, and cut into 2-4 mm thick slices while fresh, and added water twice the weight volume of the original drug, broken the cell wall for 8 minutes, filtered, and the filtrate was centrifuged at 4000 r / min for 20 minutes to obtain the first supernatant; then 4 times the amount of water was added and the cell wall was broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time were combined; Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant; (2) The supernatants obtained in the above (1) are respectively ultrafiltered using a 10000D ultrafiltration membrane to remove macromolecular substances, and the ultrafiltration permeates are collected and then concentrated using a 200D nanofiltration membrane to obtain nanofiltration concentrates; then the nanofiltration concentrates are freeze-dried, and the freeze-dried products are collected and pulverized to obtain freeze-dried powders; (3) Wash and cut the leech into sections, freeze-dry, grind into coarse powder, pass through a 100-mesh sieve, and then ultrafine grind and pass through a 300-mesh sieve to obtain leech ultrafine powder; (4) The freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3) and the crushed bezoar powder are uniformly mixed, vacuum-packed and sterilized to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

3. The method for preparing the lipid-regulating and blood-clearing Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 60 parts of fresh hawthorn, 50 parts of fresh ginseng, 60 parts of fresh gastrodia, 60 parts of fresh notoginseng, 60 parts of fresh salvia, 60 parts of fresh polygonum multiflorum, 3 parts of leech and 0.5 parts of bezoar. The preparation method is: (1) The fresh hawthorns are selected and washed twice, cut into 2-4 mm thick slices while fresh, add water 3 times the weight volume of the original drug, break the wall for 10 minutes, filter, and centrifuge the filtrate at 6000 r / min for 15 minutes to obtain the first supernatant; then add water and repeat the above method to break the wall and centrifuge once to obtain the second supernatant, and combine the supernatants; Fresh Panax notoginseng was selected and washed twice, and cut into 2-4 mm thick slices while fresh, and added with water 4 times the weight and volume of the original drug, and the cell wall was broken for 10 minutes, filtered, and the filtrate was centrifuged at 6000 r / min for 15 minutes to obtain the first supernatant; then water was added and the cell wall was broken and centrifuged again according to the above method to obtain the second supernatant, and the supernatants of each time were combined; Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant; (2) The supernatants obtained in the above (1) are respectively ultrafiltered using a 10000D ultrafiltration membrane to remove macromolecular substances, and the ultrafiltration permeates are collected and then concentrated using a 200D nanofiltration membrane to obtain nanofiltration concentrates; then the nanofiltration concentrates are freeze-dried, and the freeze-dried products are collected and pulverized to obtain freeze-dried powders; (3) Wash and cut the leech into segments, freeze-dry, grind into coarse powder, pass through a 100-mesh sieve, and then ultrafine grind and pass through a 220-mesh sieve to obtain leech ultrafine powder; (4) The freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3) and the crushed bezoar powder are uniformly mixed, vacuum-packed and sterilized to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

4. The method for preparing the lipid-regulating and blood-clearing Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 10 parts of fresh hawthorn, 10 parts of fresh ginseng, 10 parts of fresh gastrodia elata, 10 parts of fresh notoginseng, 10 parts of fresh salvia miltiorrhiza, 10 parts of fresh polygonum multiflorum, 0.2 parts of leech and 0.05 parts of bezoar. The preparation method is: (1) The fresh hawthorn is selected and washed once, cut into 2-4 mm thick slices while fresh, and water 4 times the weight volume of the original drug is added to break the wall for 20 minutes, filtered, and the filtrate is centrifuged at 6000 r / min for 30 minutes to obtain a supernatant; Fresh Panax notoginseng was selected and washed once, cut into 2-4 mm thick slices while fresh, added with water 5 times the weight and volume of the original drug, broken for 20 min, filtered, and the filtrate was centrifuged at 6000 r / min for 30 min to obtain the supernatant; Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant; (2) The supernatants obtained in the above (1) are respectively ultrafiltered using a 10000D ultrafiltration membrane to remove macromolecular substances, and the ultrafiltration permeates are collected and then concentrated using a 200D nanofiltration membrane to obtain nanofiltration concentrates; then the nanofiltration concentrates are freeze-dried, and the freeze-dried products are collected and pulverized to obtain freeze-dried powders; (3) Wash and cut the leech into sections, freeze-dry, grind into coarse powder, pass through an 80-mesh sieve, and then ultrafine grind and pass through a 150-mesh sieve to obtain leech ultrafine powder; (4) The freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3) and the crushed bezoar powder are uniformly mixed, vacuum-packed and sterilized to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

5. The method for preparing the lipid-regulating and meridian-unblocking Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 50 parts of fresh hawthorn, 20 parts of fresh ginseng, 50 parts of fresh gastrodia elata, 20 parts of fresh notoginseng, 50 parts of fresh salvia miltiorrhiza, 20 parts of fresh Polygonum multiflorum, 2.3 parts of leech and 0.4 parts of bezoar. The preparation method is: (1) After selecting the fresh hawthorn, wash it 1 to 3 times, cut it into pieces while it is fresh, add water 0.5 times the weight volume of the original drug, break the wall for 10 minutes, filter, and centrifuge the filtrate at 2000 r / min for 30 minutes to obtain the first supernatant; then add water 3 times the weight volume of the original drug, break the wall for 5 minutes, filter, and centrifuge the filtrate at 5000 r / min for 15 minutes to obtain the second supernatant, then add water and repeat the wall breaking and centrifugation twice according to the above method to obtain the third to fourth supernatants, and combine the supernatants of each time; After selection, fresh Panax notoginseng was washed 1-3 times, cut into segments while fresh, added water with a volume of 1 times the weight of the original drug, broken the cell wall for 10 minutes, filtered, and the filtrate was centrifuged at 2000 r / min for 30 minutes to obtain the first supernatant; then added water with a volume of 3 times the weight of the original drug, broken the cell wall for 5 minutes, filtered, and the filtrate was centrifuged at 5000 r / min for 15 minutes to obtain the second supernatant, and then added water and repeated the cell wall breaking and centrifugation twice according to the above method to obtain the third to fourth supernatants, and the supernatants of each time were combined; Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant; (2) The supernatants obtained in the above (1) are respectively ultrafiltered using a 10000D ultrafiltration membrane to remove macromolecular substances, and the ultrafiltration permeates are collected and then concentrated using a 200D nanofiltration membrane to obtain nanofiltration concentrates; then the nanofiltration concentrates are freeze-dried, and the freeze-dried products are collected and pulverized to obtain freeze-dried powders; (3) Wash and cut the leech into segments, freeze-dry, grind into coarse powder, pass through a 100-mesh sieve, and then ultrafine grind and pass through a 280-mesh sieve to obtain leech ultrafine powder; (4) The freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3) and the crushed bezoar powder are uniformly mixed, vacuum-packed and sterilized to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

6. The method for preparing the lipid-regulating and blood-clearing Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh hawthorn, 40 parts of fresh ginseng, 20 parts of fresh gastrodia, 50 parts of fresh notoginseng, 20 parts of fresh salvia, 50 parts of fresh Polygonum multiflorum, 0.9 parts of leech and 0.2 parts of bezoar. The preparation method is: (1) The fresh hawthorns are selected and washed three times, cut into pieces while fresh, and water twice the weight volume of the original drug is added, the cells are broken for 8 minutes, filtered, and the filtrate is centrifuged at 8000 r / min for 5 minutes to obtain the first supernatant; then water is added and the cell wall is broken and centrifuged four times according to the above method to obtain the second to fifth supernatants, and the supernatants of each time are combined; Fresh Panax notoginseng was selected and washed three times, cut into segments while fresh, added with water twice the weight and volume of the original drug, broken for 10 min, filtered, and the filtrate was centrifuged at 8000 r / min for 5 min to obtain the first supernatant; then water was added and the cell wall was broken and centrifuged four times according to the above method to obtain the second to fifth supernatants, and the supernatants of each time were combined; Prepare fresh Gastrodia elata, fresh Salvia miltiorrhiza and fresh ginseng into corresponding supernatants according to the preparation method of fresh hawthorn supernatant, and prepare fresh Polygonum multiflorum into corresponding supernatants according to the preparation method of fresh Panax notoginseng supernatant; (2) The supernatants obtained in the above (1) are respectively ultrafiltered using a 10000D ultrafiltration membrane to remove macromolecular substances, and the ultrafiltration permeates are collected and then concentrated using a 200D nanofiltration membrane to obtain nanofiltration concentrates; then the nanofiltration concentrates are freeze-dried, and the freeze-dried products are collected and pulverized to obtain freeze-dried powders; (3) Wash and cut the leech into sections, freeze-dry, grind into coarse powder, pass through an 80-mesh sieve, and then ultrafine grind and pass through a 180-mesh sieve to obtain ultrafine leech powder; (4) The freeze-dried powders obtained in step (2), the ultrafine leech powder obtained in step (3) and the crushed bezoar powder are uniformly mixed, vacuum-packed and sterilized to obtain a lipid-regulating and meridian-opening Chinese medicine composition.

7. Use of the lipid-regulating and meridian-unblocking Chinese medicine composition prepared by the method according to any one of claims 1 to 6 in the preparation of lipid-regulating and meridian-unblocking drugs.

8. Use of the lipid-regulating and blood-clearing Chinese medicine composition prepared by the method according to claim 7 in the preparation of lipid-regulating and blood-clearing drugs, characterized in that: The medicine is a granule, tablet or capsule prepared by adding a pharmaceutically acceptable auxiliary material to a lipid-regulating and blood-clearing Chinese medicine composition.

9. Use of the lipid-regulating and meridian-unblocking Chinese medicinal composition prepared by the method according to any one of claims 1 to 6 in the preparation of drugs for preventing, alleviating and treating dyslipidemia.

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