Plant source disinfection material as well as use method and application thereof

Abstract

The invention discloses a botanical disinfection material and a use method and application thereof.The disinfection material is composed of artemisia desertorum, peganum harmala, cypress shells, tea seed meal and myrcia tree, the disinfection material can achieve the disinfection and sterilization effect after being used in a small amount in a closed environment, the disinfection material is made of natural plants, and the disinfection material is easy to prepare, green, environmentally friendly and free of side effects; the method is environment-friendly and human-body-friendly; the disinfection material is good in disinfection effect, wide in sterilization spectrum and remarkable in sterilization and disinfection effect, and can be widely applied to disinfection of indoor occasions such as hospitals, hotels, residences and farms.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a plant-derived disinfection material, a use method and an application thereof. Background Art

[0002] In the biomedical field, disinfection and antibacterial methods are important measures to ensure the safety of the medical environment and prevent infection. Influenza is an acute respiratory infection caused by influenza virus. It is also a highly contagious and fast-spreading disease. The virus is mainly transmitted through droplets in the air, contact between people, or contact with contaminated objects. The existing medical protection system not only has physical isolation to prevent virus infection from the source, but also air disinfection.

[0003] The so-called air disinfection method refers to the inactivation or removal of microorganisms, viruses, bacteria, etc. in the air by physical or chemical means to achieve the purpose of disinfection and purification of the air. Common air disinfection methods include: ultraviolet disinfection, filtration disinfection, negative ion air purification disinfection, fumigation disinfection and other methods. Fumigants refer to dosage forms that use certain flammable substances to produce smoke through combustion to achieve the purpose of killing insects, sterilizing, preventing and treating diseases. Chinese herbal fumigation to avoid plague is the earliest traditional air disinfection method that has been circulated to this day and is still in clinical practice. Chinese herbal fumigation is also very consistent with the idea of ​​"preventing disease before it occurs" in traditional Chinese medicine. The Yellow Emperor's Internal Classic says: "The saint does not treat existing diseases but treats diseases before they occur." The "preventing disease before it occurs" here means taking appropriate medical means to prevent the germination or aggravation of diseases, so as to achieve the effect of preventing them before they occur. CN104886178A discloses a natural plant disinfectant fumigant and a preparation method thereof. The fumigant has simple ingredients. The raw materials such as raw atractylodes and calamus are dried and crushed to 100-180 meshes respectively, and the wormwood leaves are beaten into wormwood wool and mixed and stirred evenly to obtain the natural plant disinfectant fumigant; CN112889846A discloses a Chinese herbal disinfectant fumigant and a preparation method thereof. The fumigant is mainly made of Peganum harmala and tea dregs, and then medically acceptable auxiliary materials are added to make a soft material and press to obtain the Chinese herbal disinfectant fumigant. At present, most of the existing influenza viruses are infected by people through direct contact with objects and environments contaminated by poultry or their excrement. The poultry house disinfection room is the main means to reduce the spread of viruses among poultry. Therefore, in view of the frequent viral infectious diseases, it is particularly important to develop a disinfection method that can be widely used in indoor occasions such as hospitals, hotels, residences, poultry houses, etc. to prevent diseases, control infections, and reduce the spread of infectious diseases. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention aims to provide a plant-derived disinfection material, a method of use and application thereof, so as to solve the problems mentioned in the background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: The invention provides a plant-derived disinfection material. The disinfection material comprises the following raw materials in parts by weight: 40-60 parts of Artemisia ordosica, 25-50 parts of Peganum harmala, 5-50 parts of Cupressus bark, 1-18 parts of tea dregs and 3-45 parts of Cineraria sinensis.

[0006] As a further preferred embodiment of the present invention, the disinfection material comprises the following raw materials in parts by weight: 55 parts of Artemisia ordosica, 30 parts of Peganum harmala, 15 parts of Cupressus bark, 8 parts of tea meal, and 6 parts of Citronella.

[0007] The Artemisia ordosica in the present invention refers to Artemisia ordosica leaves; the Peganum harmala refers to Peganum harmala leaves and stems, and the weight ratio of the Peganum harmala leaves to the stems is 1:3.

[0008] A method for preparing a plant-derived disinfectant material is carried out according to the following steps: The wormwood, camellia sinensis, cypress bark, tea dregs and fragrant leaf tree are dried separately, crushed to 60-100 mesh, and then mixed and stirred evenly to prepare a powder; Or wormwood, camel grass, cypress shell, tea meal and fragrant leaf tree are dried separately, crushed to 60-100 mesh, mixed and stirred evenly, 10-20 parts of medically acceptable auxiliary materials dextrin or starch are added, and then water is added and stirred evenly to make a soft material, and then the soft material is mechanically pressed into strips or discs to obtain the disinfection material.

[0009] In addition, the present invention also provides a method for using the above disinfection material, that is, in a confined space, the dosage of the active ingredient of the disinfection material is 0.2-0.6 g / cubic meter.

[0010] The enclosed space that can be disinfected by the method of the present invention refers to various enclosed spaces such as human living rooms, office rooms, hotels, hospitals, and livestock breeding rooms.

[0011] Compared with the prior art, the present invention has the following beneficial effects: (1) This method uses Artemisia ordosica as a plant source material in the local area, and is combined with traditional antibacterial and anthelmintic Chinese herbal medicines such as Peganum harmala, tea meal, and fragrant leaf tree. Through repeated experiments, it is verified that the technical solution of the present invention has a good disinfection and sterilization effect, especially when combined with Artemisia ordosica, the sterilization effect is better than the fumigants on the market; (2) The product of the present invention is safe and harmless. The materials are all plant-derived and are harmless to humans, animals and the environment during the entire process of use. It is safer than chemical fumigants. DETAILED DESCRIPTION Example

[0012] Based on 100 g, 55 parts of Artemisia ordosica, 25 parts of Peganum harmala, 15 parts of tea meal, 45 parts of Cineraria officinalis, and 50 parts of Cypress bark are dried separately by weight, crushed to 80 mesh, mixed and stirred evenly to obtain a powder. Example

[0013] Based on 100 g, 55 parts of Artemisia ordosica, 25 parts of Peganum harmala, and 15 parts of tea dregs are dried by weight, 40 parts of Folium styracifluum and 45 parts of Cypress bark are dried by weight, and crushed to 80 mesh, mixed and stirred evenly to obtain a powder. Example

[0014] Based on 100 g, 60 parts of Artemisia ordosica, 30 parts of Peganum harmala, and 10 parts of tea meal are dried by weight, 35 parts of Fragrant Leaf Tree and 45 parts of Cupressus chinensis are dried by weight, and crushed to 100 mesh, mixed and stirred evenly to obtain a powder. Example

[0015] Based on 100 g, 50 parts of Artemisia ordosica, 40 parts of Peganum harmala, and 15 parts of tea dregs are dried by weight, 10 parts of Folium styracifluum and 25 parts of Cypress bark are dried by weight, and crushed to 100 mesh, mixed and stirred evenly to obtain a powder. Example

[0016] Based on 100 g, 45 parts of Artemisia ordosica, 50 parts of Peganum harmala, and 12 parts of tea meal are dried by weight, 3 parts of Fragrant Tree and 30 parts of Cupressus bark are dried by weight, and crushed to 120 mesh, mixed and stirred evenly to obtain a powder. Example

[0017] Taking 100 g as the base, 55 parts of Artemisia ordosica, 30 parts of Peganum harmala, and 8 parts of tea meal are dried respectively by weight, 6 parts of Fragrant Leaf Tree and 15 parts of Cupressus chinensis are dried respectively, and crushed to 80 mesh, mixed and stirred evenly, 20 parts of auxiliary material starch are added, and then water is added and stirred evenly to make a soft material, and then the soft material is mechanically pressed into strips with a diameter of 5 mm, and dried to obtain a disc-shaped fumigant. Example

[0018] Taking 100 g as the base, 48 parts of Artemisia ordosica, 30 parts of Peganum harmala, and 2 parts of tea meal are dried respectively by weight, 8 parts of Folium spatholobi and 30 parts of Cypress bark are dried respectively, and crushed to 100 mesh, mixed and stirred evenly, 25 parts of auxiliary material starch are added, and then water is added and stirred evenly to make a soft material, and then the soft material is mechanically pressed into strips with a diameter of 3 mm, and dried to obtain a disc-shaped fumigant. Example

[0019] Taking 100 g as the base, 40 parts of Artemisia ordosica, 25 parts of Peganum harmala, and 5 parts of tea meal are dried respectively by weight, 5 parts of Fragrant Leaf Tree and 45 parts of Cupressus chinensis are dried respectively, and crushed to 120 mesh, mixed and stirred evenly, 15 parts of auxiliary material starch are added, and then water is added and stirred evenly to make a soft material, and then the soft material is mechanically pressed into strips with a diameter of 6 mm, and dried to obtain a disc-shaped fumigant. Example

[0020] The samples prepared above were tested and determined: 1. Quantitative bactericidal test of fumigants against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa: Test strains: Staphylococcus aureus ATCC6538, Escherichia coli ATCC8099 and Pseudomonas aeruginosa ATCC15442, provided by China General Microbiological Culture Collection Center. The above strains are of the fifth generation, and the bacterial suspension is prepared with PBS.

[0021] Test method: 1. Preparation of bacterial slices: According to the Ministry of Health's "Disinfection Technical Specifications" (2002 edition) 2.1.1.2.4, use cloth as the carrier, and the bacterial count is 5x10 5 cfu / piece-5x10 6 cfu / piece.

[0022] 2. Carrier quantitative sterilization test: According to 2.1.1.7 of the "Technical Specifications for Disinfection" of the Ministry of Health. The test was carried out in four sealed glass boxes of the same size (0.7mx0.7mx0.7m, three as experimental groups and one as control group). Foam blocks with artificially stained cloth pieces were placed at the upper 1 / 3 and lower 1 / 3 of the diagonal line in the sealed glass box, and the fumigant was lit in the middle of the bottom of the box. One fumigant prepared in Example 6 was lit in the experimental box, and no incense was lit in the control box. The effect was 100 minutes, and the temperature was 22℃±1℃. The test was repeated 3 times.

[0023] The test results are shown in Table 1. Under the test temperature of 22℃±1℃, after three repeated tests, it was proved that in a closed glass box (0.7mx0.7mx0.7m), one fumigant was ignited and fumigated for 100 minutes, and the killing rate of Staphylococcus aureus stained on the cloth was 99.83%, the killing rate of Escherichia coli was 99.99%, and the killing rate of Pseudomonas aeruginosa was 99.99%.

[0024] Table 1 Quantitative bactericidal test of fumigants against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

[0025] 2. Field test of air disinfection with fumigants: The instruments used are a six-stage mesh air impact sampler (No.: 202144CD0115) and a constant temperature incubator (No.: 201044BJ0028).

[0026] Test method: Randomly select a room (volume is about 25 m 3 , with an area of ​​about 10 m 2), a six-stage sieve air impact sampler was placed in the center of the room at a height of 1.0m from the ground for sampling. The sampling flow rate was 28.3 L / min and the sampling time was 424s, which was used as the pre-disinfection sample (positive control). According to the product instructions, 7 fumigants prepared in Example 6 were ignited and fumigated for 100min to disinfect the room. After the disinfection, the same method was used to sample again, with a sampling flow rate of 28.3 L / min and a sampling time of 424s, as the test sample after disinfection, and live bacteria culture counts were performed. The test was repeated 3 times, and the fumigant developed by the existing CN112889846A patent was used as the control.

[0027] The test results are shown in Table 2. After three repeated tests, it was shown that at room temperature, 7 fumigants were ignited and fumigated to a volume of about 25 m 3 , an area of ​​about 10m 2 The room was disinfected for 100 minutes, and the average elimination rate of natural bacteria in the air was 97.12%, while the average elimination rate of natural bacteria in the air after using the control group fumigant was only 76.26%.

[0028] Table 2 Test on the disinfection effect of fumigants on natural bacteria in the air

[0029] 3. Killing rate of fumigants against Staphylococcus aureus (simulated field test): The test method is the second part (2.1.3) of the Technical Specifications for Disinfection (2002 edition of the Ministry of Health), and the test strain is Staphylococcus aureus ( Staphylococcus albus ) 8032, the test conditions are as follows: 7 fumigant samples prepared in Example 6 are ignited and placed in a test chamber (20m 3 ) The test environment was located in the middle of the space, the relative humidity was 65.4%, the temperature was 24.6°C, and the test was continued for the prescribed time of 1 hour. Samples were taken using a culture medium plate containing a neutralizer.

[0030] Table 3 shows the killing rate of fumigants against white grape bacteria (simulated field test). The results show that the sample prepared in Example 6 3 The disinfection effect in the test chamber was tested for 1 hour, and the test was repeated 3 times. The killing rate of Staphylococcus aureus was greater than 99.9%, which was in line with the evaluation requirements in the second part (2.1.3.4) of the "Technical Specifications for Disinfection" (Ministry of Health, 2002 edition) (killing rate ≥ 99.9%). It was determined that the sample was qualified for disinfection of the strains tested in the air under the test conditions used.

[0031] Table 3 Killing rate of fumigants against white grape fungus (simulated field test)

[0032] Note: The results of the killing rate test have eliminated the influence of the natural extinction of microorganisms in the air. The killing rate of Staphylococcus aureus was tested in the laboratory. The test method and test strains were the same as those in the simulated field test. The test conditions were as follows: 3 fumigant samples prepared in Example 6 were ignited and placed in a test chamber (1m 3 ), the relative humidity of the test environment is 60.6%, the test environment temperature is 24.8℃, the action is for the specified time of 1h, and sampling is carried out with a sampling liquid containing a neutralizer.

[0033] Table 4 shows the killing rate of fumigants against white grape bacteria (laboratory test). The results show that the sample prepared in Example 6 3 The disinfection effect in the test chamber was tested for 1 hour, and the test was repeated 3 times. The killing rate of Staphylococcus aureus was greater than 99.9%, which was in line with the evaluation requirements in the second part (2.1.3.4) of the "Technical Specifications for Disinfection" (Ministry of Health, 2002 edition) (killing rate ≥ 99.9%). It was determined that the sample was qualified for disinfection of the strains tested in the air under the test conditions used.

[0034] Table 4 Killing rate of fumigants against white grape fungus (laboratory test)

[0035] Note: The results of the killing rate test have eliminated the influence of the natural extinction of microorganisms in the air. 4. Acute inhalation toxicity test of fumigants on rats: The animals were acclimatized in a barrier environment animal room for 7 days before the test. The fumigant prepared in Example 6 was selected. During the test, the animals were weighed and marked. A 60.0 g sample was placed in a static poison sample volatilization device and ignited to volatilize the sample. After the experiment, the sample was taken out and weighed again to 27.1 g. The ash weight after burning was 2.4 g. The poison dose was calculated to be 101666.7 mg / m 3 . Inhalation exposure for 4 hours. Observe and record the exposure process and the poisoning and death of animals during the observation period, which is generally 14 days. After the observation period, the surviving animals were killed and gross autopsied. (Note: The breeding environment is a barrier environment with a temperature of 22.8℃-23.6℃ and a humidity of 45.8%-54.8%; the weight of mice is 182.1g-217.1g, and the weight of the same sex does not exceed ±20% of the mean; the name of the feed is the maintenance feed for irradiated sterilized experimental mice, and the feed production license number is Su Feed Certificate (2019) 01008; the animal drinking water is first-level RO ultrafiltered water, which is chlorinated to control the free chlorine content of the water at 0.3mg / L-2mg / L).

[0036] Table 5 shows the results of the acute inhalation toxicity test of fumigants on rats. The results show that the experimental animals did not show any poisoning symptoms or death within 14 days of exposure; the weight of male and female animals was normal (see Table 1). At the end of the experimental observation, the gross anatomical examination of the test animals also showed no abnormal changes. LC 50 >101666.7 mg / m 3 .

[0037] Table 5 Acute inhalation toxicity test of fumigants on rats

[0038] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A plant-derived disinfection material, characterized in that: The invention is composed of the following raw materials in parts by weight: 40-60 parts of Artemisia ordosica, 25-50 parts of Peganum harmala, 5-50 parts of Cypress bark, 1-18 parts of tea dregs and 3-45 parts of Folium strychnos.

2. A plant-derived disinfection material according to claim 1, characterized in that: The invention is composed of the following raw materials in parts by weight: 55 parts of Artemisia ordosica, 30 parts of Peganum harmala, 15 parts of Cypress bark, 8 parts of tea dregs and 6 parts of Folium strychnifolium.

3. A plant-derived disinfection material according to claim 1, characterized in that: The Peganum harmala comprises Peganum harmala leaves and Peganum harmala stems, and the weight ratio of the Peganum harmala leaves to the Peganum harmala stems is 1:

3.

4. A plant-derived disinfection material as claimed in claim 1, characterized in that: In a confined space, the dosage of the active ingredient of the disinfection material is 0.2-0.6 g / cubic meter.

5. Use of a plant-derived disinfectant material as claimed in claim 1 in an environmental disinfection and sterilization preparation.

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