A tooth polishing agent with antibacterial efficacy and its preparation method

Abstract

The present invention relates to the field of dental care, and particularly relates to an antibacterial tooth polishing agent and a preparation method thereof, comprising the following raw materials: silica, sodium carboxymethyl cellulose, xanthan gum, propylene glycol, cocamidopropyl betaine, glycerol, modified nano-zinc oxide, modified nano-hydroxyapatite, glucono delta-lactone, stevioside, sodium benzoate. In the present invention, the modified nano-zinc oxide, modified nano-hydroxyapatite and glucono delta-lactone act through a synergistic effect. The modified nano-hydroxyapatite acts as a carrier to adsorb chemical substances such as modified nano-zinc oxide to form rod-shaped nano-microcapsules, and forms a dense and uniform protective layer on the tooth surface through the nano-size effect. Glucono delta-lactone promotes the slow release of the rod-shaped nano-microcapsules. The three form a dynamic balance system, continuously exerting antibacterial and remineralization effects, strengthening the tooth structure, adjusting the pH value to maintain the oral environment, and providing comprehensive and long-term protection for oral health.

Description

Technical Field

[0001] The present invention relates to the field of dental care, and specifically to a tooth polishing agent with antibacterial efficacy and a preparation method thereof. Background Art

[0002] Tooth polishing agents are products specifically used for dental care, mainly for cleaning and polishing the tooth surface, usually used during professional dental cleaning in dental clinics. Their main purpose is to remove stains, plaque, and slight discoloration on the tooth surface, making the tooth surface smoother and shinier. The initial tooth polishing materials were mainly powders such as calcium carbonate and quartz powder, but these materials had limited effects and were prone to damaging tooth enamel. Subsequently, finer abrasive materials such as alumina and silica were introduced, which improved the polishing effect and reduced the potential harm to teeth. However, these traditional tooth polishing agents mainly remove stains and plaque through physical means and only focus on the cleaning and whitening effects of the products on teeth. With the continuous progress of technology, people's demand for oral health is also increasing. Tooth polishing agents with antibacterial efficacy show unique advantages. In addition to the traditional cleaning and polishing functions, antibacterial tooth polishing agents can effectively inhibit the growth of oral bacteria, providing more comprehensive protection for oral health. The antibacterial components can form a protective film on the tooth surface and continuously play an antibacterial role for a long time after polishing. At the same time, by inhibiting the growth of harmful bacteria, the risk of oral diseases such as tooth decay and gingivitis can be reduced, and bad breath can also be improved by reducing the bacteria causing bad breath, helping to keep the breath fresh. In addition, for people with oral sensitivity or prone to inflammation, antibacterial tooth polishing agents can also provide milder protection. Therefore, it is of great significance to develop a safe and effective antibacterial tooth polishing agent.

[0003] (1) Technical Problems to be Solved

[0004] The purpose of the present invention is to provide a tooth polishing agent with antibacterial efficacy and a preparation method thereof. By optimizing the material formula of the tooth polishing agent and combining modern nanotechnology, the antibacterial efficacy of the tooth polishing agent can be effectively improved, and a more persistent and effective antibacterial environment can be provided for the oral cavity. While ensuring the polishing effect, the tooth structure can be further strengthened by repairing minor defects in tooth enamel, enhancing tooth strength, effectively preventing oral diseases, and providing long-term protection for oral health.

[0005] (2) Technical Solutions

[0006] To achieve the above object, on the one hand, the present invention provides a tooth polishing agent with antibacterial efficacy, comprising the following raw materials in parts by weight: 8-12 parts of silica, 2-4 parts of sodium carboxymethyl cellulose, 1-2 parts of xanthan gum, 1-2 parts of propylene glycol, 2-4 parts of cocamidopropyl betaine, 4-6 parts of glycerol, 0.5-1 part of modified nano-hydroxyapatite, 1-2 parts of glucono delta-lactone, 1-2 parts of stevioside, 2-4 parts of sodium benzoate;

[0007] The tooth polishing agent with antibacterial efficacy further comprises:

[0008] Modified nano-zinc oxide;

[0009] The weight ratio of the modified nano-zinc oxide to silica is 1:(8-12);

[0010] The modified nano-zinc oxide is obtained by surface coupling with tetraphenylporphyrin, and the particle size of the modified nano-zinc oxide is 40-60 nm, and the specific surface area is 120-150 m 2 / g.

[0011] Furthermore, the preparation method of the modified nano-zinc oxide comprises:

[0012] S11. Dissolve zinc acetate in purified water under stirring, after stirring for 0.5-1 h, slowly add 0.1 mol / L sodium hydroxide solution, raise the temperature to 55-65 °C, and continue stirring and reacting for 4-6 h to obtain a first mixed solution;

[0013] S12. Perform high-speed centrifugal separation on the first mixed solution, with a rotation speed of 6000-8000 rpm, centrifuge for 10-15 min, wash the separated solid with purified water 3 times and then perform vacuum drying, with a drying temperature of 50-60 °C, after drying for 12-14 h, obtain zinc oxide and grind it into powder for standby;

[0014] S13. Under nitrogen protection, disperse zinc oxide in absolute ethanol and perform ultrasonic treatment, with an ultrasonic frequency of 40-50 kHz, after ultrasonic treatment at room temperature for 0.5-1 h, slowly add 3-aminopropyltriethoxysilane and reflux, with a reflux temperature of 75-80 °C, after refluxing for 6-8 h, obtain a second mixed solution;

[0015] S14. Perform high-speed centrifugal separation on the second mixed solution, with a rotation speed of 8000-10000 rpm, centrifuge for 15-20 min, wash the separated solid with absolute ethanol 3 times and then perform vacuum drying, with a drying temperature of 45-55 °C, after drying for 8-12 h, obtain a nano-zinc oxide intermediate and grind it into powder for standby;

[0016] S15. Dissolve tetraphenylporphyrin in N,N-dimethylformamide under stirring. After stirring for 1 - 2 h, slowly add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide. Heat up to 45 - 60 °C and continue stirring for 4 - 6 h to obtain a third mixed solution;

[0017] S16. Under light avoidance and nitrogen protection, disperse the nano-zinc oxide intermediate in N,N-dimethylformamide. After stirring for 0.5 - 1 h, slowly add the third mixed solution and continue stirring at room temperature for 24 - 28 h to obtain a fourth mixed solution;

[0018] S17. Centrifuge the fourth mixed solution at a high speed at a rotation speed of 10000 - 12000 rpm for 15 - 20 min. Wash the separated solid alternately with N,N-dimethylformamide and absolute ethanol three times and then carry out vacuum drying at a drying temperature of 40 - 50 °C for 12 - 14 h to obtain modified nano-zinc oxide and grind it into a powder for standby.

[0019] Further, the mass ratio of zinc acetate to tetraphenylporphyrin is 1:(0.01 - 0.1).

[0020] Further, the mass ratio of tetraphenylporphyrin, N,N-dimethylformamide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide is 1:(2 - 4):(0.5 - 1):(0.5 - 1).

[0021] Further, the preparation method of the modified nano-hydroxyapatite includes:

[0022] S21. Dissolve calcium dihydrogen phosphate and calcium hydroxide in purified water under stirring. After stirring for 0.5 - 1 h, slowly add cetyltrimethylammonium bromide, and at the same time adjust the pH value to 10.5 - 11 with ammonia water solution. Continue stirring for 1 - 2 h to obtain a fifth mixed solution;

[0023] S22. Transfer the fifth mixed solution to a hydrothermal reaction kettle for hydrothermal reaction at a reaction temperature of 180 - 200 °C for 12 - 15 h to obtain a sixth mixed solution;

[0024] S23. Centrifuge the sixth mixed solution at a high speed at a rotation speed of 10000 - 12000 rpm for 10 - 15 min. Wash the separated solid alternately with purified water and absolute ethanol three times and then carry out freeze-drying at a drying temperature of -40 - -60 °C for 20 - 24 h to obtain rod-shaped nano-hydroxyapatite and grind it into a powder for standby;

[0025] S24. Dissolve sodium fluoride in purified water under stirring to obtain a sodium fluoride solution. Disperse rod-shaped nano-hydroxyapatite and γ-methacryloxypropyltrimethoxysilane in purified water and perform ultrasonic treatment. The ultrasonic frequency is 40 - 50 kHz. After ultrasonic treatment at room temperature for 0.5 - 1 h, slowly add the sodium fluoride solution, control the dropping rate at 1 - 2 mL / min, and continue stirring at room temperature for 4 - 6 h to obtain a seventh mixed solution;

[0026] S25. Perform high-speed centrifugal separation on the seventh mixed solution at a rotation speed of 10000 - 12000 rpm for 15 - 20 min. Wash the separated solid with purified water 5 times and then perform freeze-drying. The drying temperature is -40 - -60 °C. After drying for 20 - 24 h, obtain modified nano-hydroxyapatite and grind it into a powder for standby.

[0027] Further, the modified nano-hydroxyapatite is obtained by surface modification with sodium fluoride, and its shape is rod-shaped, with a particle size length of 60 - 80 nm, a diameter of 15 - 25 nm, and a specific surface area of 180 - 250 m 2 / g.

[0028] Further, the mass ratio of calcium dihydrogen phosphate, calcium hydroxide, and sodium fluoride is (1 - 1.5):(1.5 - 2):(0.02 - 0.1).

[0029] On the other hand, based on the same inventive concept, the present invention also provides a preparation method of a tooth polishing agent with antibacterial efficacy, which is applied to the tooth polishing agent with antibacterial efficacy described above, and includes the following steps:

[0030] S31. Add purified water to a container, and successively add sodium carboxymethyl cellulose, glycerol, xanthan gum, and propylene glycol and stir. At the same time, perform water bath heating at a heating temperature of 70 - 85 °C until fully dissolved to obtain an eighth mixed solution;

[0031] S32. Transfer the eighth mixed solution to a vacuum emulsifier, and then slowly add cocamidopropyl betaine. After emulsifying for 5 - 10 min, slowly add silica, modified nano-zinc oxide, modified nano-hydroxyapatite, glucono-delta-lactone, stevioside, and sodium benzoate. The stirring speed is 2000 - 4000 rpm, and continue emulsifying for 25 - 30 min to obtain a ninth mixed solution;

[0032] S33. Perform vacuum defoaming on the ninth mixed solution, set the pressure at -0.05 - -0.08 MPa, and after defoaming for 15 - 20 min, obtain a uniform and delicate paste, which is the tooth polishing agent.

[0033] Further, the viscosity of the tooth polishing agent is 20000 - 30000 mPa·s, and the specific gravity is 1.2 - 1.5 g / cm3 and the pH value is between 6.8 and 7.2.

[0034] The mechanism of action of the above raw material components is as follows:

[0035] Silicon dioxide (SiO 2 ) is a network structure formed by silicon and oxygen atoms connected in a tetrahedral structure. It is usually a white powder. Synthetic fine silicon dioxide particles are usually used in tooth polishes. As a mild abrasive, it can effectively remove stains and plaque on the tooth surface. At the same time, the hardness of silicon dioxide is moderate, and it will not excessively wear the tooth enamel while cleaning the teeth. Propylene glycol (CH 3 CH(OH)CH 2 OH) is a colorless, odorless, sweet and viscous liquid. In tooth polishes, it mainly acts as a humectant and solvent, preventing the product from drying or hardening during use and storage, and at the same time helping to dissolve and disperse other components, improving the touch and spreadability of the product. Glycerol (C 3 H 8 O 3 ) is a polyhydroxy alcohol with strong hygroscopicity, capable of absorbing and retaining moisture. Its good lubricity and solubility can dissolve a variety of organic and inorganic substances. In tooth polishes, it acts as a humectant and lubricant, preventing the product from drying, maintaining appropriate humidity, increasing the smoothness of the product, and improving the taste and use experience.

[0036] Sodium carboxymethyl cellulose ((C 6 H 7 O 2 (OH) 2 CH 2 COONa) n ) is a linear polymer obtained by carboxymethylation and sodiumation of cellulose. It is a white to light yellow odorless and tasteless powder, easily soluble in water. In tooth polishes, it mainly acts as a thickener and stabilizer, increasing the viscosity of the product, preventing various components from separating or precipitating, and at the same time forming a uniform gel structure, making the tooth polish easy to apply and use. Xanthan gum ((C 35 H 49 O 29 ) n ) is a high molecular polysaccharide composed of glucose, mannose and glucuronic acid. The main chain is composed of D-glucose connected by β-1,4. A trisaccharide side chain is connected to every two glucose units. It is a beige or light yellow powder, easily soluble in water. In tooth polishes, it acts synergistically with sodium carboxymethyl cellulose to further improve the consistency and stability of the product, provide good suspension, prevent solid particles (such as abrasives) from settling, enhance the adhesion of the product, and enable it to better stay on the toothbrush and tooth surface.

[0037] Cocamidopropyl betaine (C 19 H 38 N 2 O 3 ) is an amphoteric surfactant formed by the reaction of coconut oil fatty acid, 3-dimethylaminopropylamine and chloroacetic acid. It is a light yellow viscous liquid or paste, easily soluble in water. As the main cleaner and foaming agent in tooth polishing agents, it gently removes dirt and plaque on the tooth surface and produces moderate foam, enhancing the cleaning feeling and user experience. At the same time, the molecular structure of cocamidopropyl betaine contains a hydrophobic fatty acid chain from coconut oil and a hydrophilic betaine group, enabling cocamidopropyl betaine to maintain good surface activity under different pH conditions and having little irritation to oral mucosa. Stevioside (C 38 H 60 O 18 ) is a natural sweetener extracted from the leaves of the Stevia rebaudiana plant in South America. In tooth polishing agents, it can improve the taste of the product and mask the unpleasant taste brought by other ingredients. This diterpene glycoside compound contains no calories and is not digested and absorbed by the human body, so it will not cause tooth decay and is suitable for long-term use. Sodium benzoate (C 7 H 5 NaO 2 ) is a white powder or granule, easily soluble in water. It is mainly used as a preservative. By reducing the pH value inside microbial cells, interfering with the metabolic process of microorganisms, it inhibits the growth of bacteria, yeasts and molds, and extends the shelf life of the product.

[0038] Nano-zinc oxide (ZnO) is an inorganic compound with broad-spectrum antibacterial activity. Modified nano-zinc oxide is obtained by coupling tetraphenylporphyrin on the surface of nano-zinc oxide. Tetraphenylporphyrin is a chemical structure in which four pyrrole rings are connected by methylene bridges to form a large ring, with four phenyl groups connected to the periphery. It is an important macrocyclic conjugated compound. As a common photosensitizer, tetraphenylporphyrin can absorb visible light and generate more reactive oxygen species, thereby enhancing the antibacterial efficiency of modified nano-zinc oxide under natural light. Nano-hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 ) has a structure and composition similar to natural tooth enamel, has high biocompatibility, can penetrate into tiny defects in tooth enamel, promote tooth enamel remineralization, and repair tooth damage. Modified nano-hydroxyapatite is prepared by modifying sodium fluoride on the surface of rod-shaped nano-hydroxyapatite, which can further increase its binding ability and permeability to tooth enamel, while enhancing its acid resistance and remineralization ability. Glucono delta-lactone (C 6 H 10 O 6) is the cyclic lactone form of glucose. In an aqueous solution, glucono delta-lactone will slowly hydrolyze into gluconic acid, thereby gently reducing the pH value of the solution. This slow acidification process makes it an ideal pH regulator that can adjust the local environment without irritating the oral cavity.

[0039] In a tooth polishing agent with antibacterial efficacy, modified nano-zinc oxide, modified nano-hydroxyapatite, and glucono delta-lactone act through a synergistic effect. During the preparation of the tooth polishing agent, the modified nano-hydroxyapatite obtained by the hydrothermal synthesis method can be used as a carrier to adsorb chemical substances such as modified nano-zinc oxide, silica, and sodium carboxymethylcellulose to form rod-shaped nano-microcapsules due to its high specific surface area and abundant pore structure. When this tooth polishing agent is applied to the oral environment, glucono delta-lactone forms gluconic acid through slow hydrolysis, and its surface carboxyl group (-COOH) creates a slightly acidic microenvironment, prompting the slow release of chemical substances such as modified nano-zinc oxide, silica, and sodium carboxymethylcellulose from the rod-shaped nano-microcapsules. Modified nano-zinc oxide directly releases zinc ions, and the tetraphenylporphyrin coupled to its surface further enhances the binding to the bacterial cell membrane. The two kill bacteria by directly contacting and destroying the bacterial cell membrane function. At the same time, tetraphenylporphyrin absorbs visible light, causing modified nano-zinc oxide to generate electron-hole pairs under light illumination, and then generating superoxide anions and hydroxyl radicals, providing continuous and efficient antibacterial effects in the oral environment. Modified nano-zinc oxide and modified nano-hydroxyapatite complement each other, further forming a denser and more uniform protective layer on the tooth surface through the nano-size effect, effectively blocking bacteria from contacting the tooth surface. Modified nano-zinc oxide provides antibacterial protection, reducing acidic substances produced by harmful bacteria, while modified nano-hydroxyapatite promotes the remineralization of tooth enamel at the same time. By filling tiny defects on the tooth surface, it enhances the tooth strength, and at the same time releases calcium, phosphorus, and fluoride ions to further improve the oral environment. At the same time, the presence of glucono delta-lactone avoids the agglomeration of modified nano-zinc oxide and modified nano-hydroxyapatite, improves their dispersibility and stability, and the rod-shaped nano-microcapsules enable modified nano-zinc oxide to continuously play a role through the slow release effect. This not only enhances the antibacterial activity of modified nano-zinc oxide but also optimizes the remineralization process of modified nano-hydroxyapatite, and further accelerates the hydrolysis of glucono delta-lactone. The three components interact with each other to form a dynamic balance system, continuously playing antibacterial and remineralization roles. The antibacterial effect of modified nano-zinc oxide reduces harmful bacteria, the remineralization effect of modified nano-hydroxyapatite repairs teeth, strengthens the tooth structure, and enhances the tooth strength. Glucono delta-lactone maintains the oral environment by adjusting the pH value, effectively preventing oral diseases, and providing comprehensive and long-term protection for oral health.

[0040] (3) Beneficial effects

[0041] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0042] 1. Modified nano-zinc oxide, modified nano-hydroxyapatite and glucono-delta-lactone act through a synergistic effect. The modified nano-hydroxyapatite acts as a carrier to adsorb chemical substances such as modified nano-zinc oxide, silica and sodium carboxymethylcellulose to form rod-shaped nano-microcapsules;

[0043] 2. Glucono-delta-lactone promotes the slow release of the rod-shaped nano-microcapsules. The modified nano-zinc oxide directly releases zinc ions and directly contacts the tetraphenylporphyrin coupled to its surface to kill bacteria. The tetraphenylporphyrin absorbs visible light to generate electron-hole pairs, generating superoxide anions and hydroxyl radicals, further providing a continuous and efficient antibacterial effect for the oral environment;

[0044] 3. The modified nano-zinc oxide and the modified nano-hydroxyapatite form a dense and uniform protective layer on the tooth surface through the nano-size effect, effectively blocking bacteria from contacting the tooth surface. The modified nano-zinc oxide provides antibacterial protection, and the modified nano-hydroxyapatite simultaneously promotes the remineralization of tooth enamel, releasing calcium, phosphorus and fluoride ions to further improve the oral environment;

[0045] 4. Glucono-delta-lactone avoids the agglomeration of the modified nano-zinc oxide and the modified nano-hydroxyapatite, improves the dispersibility and stability of the two, forms a dynamic equilibrium system, continuously exerts antibacterial and remineralization effects, and adjusts the pH value to maintain the oral environment, providing comprehensive and long-term protection for oral health. Description of the Drawings

[0046] Figure 1 It is the SEM image of the modified nano-hydroxyapatite in Example 1 of the present invention;

[0047] Figure 2 It is the SEM image of the modified nano-zinc oxide in Example 1 of the present invention. Detailed Embodiments

[0048] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0049] The test equipment and preparations for the following examples are as follows: electronic balance (Sartorius, Germany), electrothermal constant temperature water bath (Kedao, Jiangsu), magnetic stirrer (Meiyingpu, Shanghai), ultrasonic instrument (Yixin, Shanghai), high-speed centrifuge (Jidi, Guangzhou), vacuum homogenizing emulsifier (Nuoze, Shanghai), vacuum drying oven (Jiecheng, Shanghai), rotary evaporator (Yaote, Shanghai), hydrothermal reaction kettle (Beijiaer, Shanghai), scanning electron microscope (Zeiss, Germany), specific surface area analyzer (Beijing Beishide Instrument Technology), freeze dryer (Pudong Freeze Drying, Shanghai), pH meter (Yidian, Shanghai), constant temperature incubator (Hetian, Shanghai); chemical drugs and reagents were purchased from Sigma-Aldrich.

[0050] Example 1: This example discloses a tooth polishing agent with antibacterial efficacy, which comprises the following raw materials in parts by weight: 10 parts of silicon dioxide, 3 parts of sodium carboxymethyl cellulose, 1.5 parts of xanthan gum, 1.5 parts of propylene glycol, 3 parts of cocamidopropyl betaine, 5 parts of glycerol, 0.8 part of modified nano-hydroxyapatite, 1.5 parts of glucono delta-lactone, 1.5 parts of stevioside, 3 parts of sodium benzoate. The tooth polishing agent with antibacterial efficacy also comprises modified nano-zinc oxide, and the weight ratio of the modified nano-zinc oxide to silicon dioxide is 1:10. The modified nano-zinc oxide is obtained by surface coupling with tetraphenylporphyrin, and the particle size of the modified nano-zinc oxide is 40 - 60 nm, and the specific surface area is 120 - 150 m 2 / g.

[0051] In the tooth polishing agent with antibacterial efficacy, the modified nano-zinc oxide, modified nano-hydroxyapatite and glucono delta-lactone play a role through a synergistic effect. Figure 1 The rod-like structure of the modified nano-hydroxyapatite can be clearly seen. During the preparation of the tooth polishing agent, the modified nano-hydroxyapatite obtained by hydrothermal synthesis method can be used as a carrier to adsorb chemical substances such as modified nano-zinc oxide, silicon dioxide and sodium carboxymethyl cellulose to form rod-like nano-microcapsules due to its high specific surface area and rich pore structure. When the tooth polishing agent is applied to the oral environment, glucono delta-lactone slowly hydrolyzes to form gluconic acid (C 6 H 12 O 7 ), and the carboxyl group (-COOH) on its surface dissociates to release H + , thus creating a slightly acidic microenvironment. The acidic environment promotes the slow release of chemical substances such as modified nano-zinc oxide, silicon dioxide and sodium carboxymethyl cellulose from the rod-like nano-microcapsules. The modified nano-zinc oxide directly releases zinc ions (Zn 2+), and the surface-coupled tetraphenylporphyrin is a macrocyclic compound. Its hydrophobic aromatic ring structure makes it easy to interact with the phospholipid bilayer membrane of bacteria, which further enhances the binding to the bacterial cell membrane. The two kill bacteria by direct contact by disrupting the function of the bacterial cell membrane and interfering with the physiological functions of bacteria. At the same time, tetraphenylporphyrin absorbs visible light, which excites the modified nano-zinc oxide to generate electron-hole pairs under light illumination, and then generates reactive oxygen species such as superoxide anion (O 2 - ) and hydroxyl radical (·OH), causing oxidative stress in bacteria, leading to oxidative damage to bacterial DNA, proteins and lipids, and ultimately resulting in bacterial death. The presence of modified nano-zinc oxide ensures the continuous release of zinc ions (Zn 2+ ), and the photocatalytic reaction ensures the long-term generation of reactive oxygen species, which provides a continuous and efficient antibacterial effect for the oral environment. Figure 2 Figure shows the SEM image of modified nano-zinc oxide. It can be seen that the particle size of modified nano-zinc oxide is uniform and its morphology is spherical. Modified nano-zinc oxide and modified nano-hydroxyapatite complement each other, and further form a denser and more uniform protective layer on the tooth surface through the nano-size effect, effectively blocking bacteria from contacting the tooth surface. Modified nano-zinc oxide provides antibacterial protection and reduces the acidic substances produced by harmful bacteria, while modified nano-hydroxyapatite releases calcium ions (Ca 2 +), phosphate ions (PO 4 3- ) and fluoride ions (F -)Promote the remineralization of tooth enamel, fill the tiny defects on the tooth surface, while enhance the resistance of tooth enamel to acid, strengthen the tooth strength, further improve the oral environment. At the same time, the presence of gluconolactone avoids the agglomeration of modified nano-zinc oxide and modified nano-hydroxyapatite. The long-chain structure of gluconic acid molecules produced by hydrolysis will form a steric hindrance on the surface of nanoparticles, preventing the aggregation of nanoparticles through steric hindrance effect. At the same time, the adsorbed gluconic acid molecules also increase the negative charge on the surface of nanoparticles, resulting in electrostatic repulsion between nanoparticles, thereby improving the dispersibility and stability of the two, ensuring the structural integrity of modified nano-zinc oxide and modified nano-hydroxyapatite. And the rod-shaped nano-microcapsules enable the modified nano-zinc oxide to continuously play a role through slow release. This not only enhances the antibacterial activity of modified nano-zinc oxide, but also further optimizes the remineralization process of modified nano-hydroxyapatite due to the reduction of bacteria, and further accelerates the hydrolysis of gluconolactone. The gluconic acid molecules produced by hydrolysis continuously regulate the pH value of the local oral environment. The three components interact with each other to form a dynamic balance system, continuously playing antibacterial and remineralization roles. The antibacterial effect of modified nano-zinc oxide reduces harmful bacteria, the remineralization effect of modified nano-hydroxyapatite repairs teeth, strengthens the tooth structure and enhances the tooth strength, and gluconolactone maintains the oral environment by regulating the pH value, effectively preventing oral diseases and providing comprehensive and long-term protection for oral health.

[0052] The preparation method of the modified nano-zinc oxide includes:

[0053] S11. Dissolve zinc acetate in purified water under stirring, after stirring for 0.5 - 1 h, slowly add 0.1 mol / L sodium hydroxide solution, raise the temperature to 55 - 65 °C, and continue stirring and reacting for 4 - 6 h to obtain a first mixed solution;

[0054] S12. Centrifuge the first mixed solution at a high speed, with a rotation speed of 6000 - 8000 rpm, centrifuge for 10 - 15 min. Wash the separated solid with purified water 3 times and then perform vacuum drying. The drying temperature is 50 - 60 °C, and after drying for 12 - 14 h, obtain zinc oxide and grind it into powder for standby;

[0055] S13. Under nitrogen protection, disperse zinc oxide in absolute ethanol and perform ultrasonic treatment. The ultrasonic frequency is 40 - 50 kHz. After ultrasonic treatment at room temperature for 0.5 - 1 h, slowly add 3-aminopropyltriethoxysilane and reflux. The reflux temperature is 75 - 80 °C, and after refluxing for 6 - 8 h, obtain a second mixed solution;

[0056] S14. Centrifuge the second mixed solution at a high speed at a rotation speed of 8000 - 10000 rpm for 15 - 20 minutes. Wash the separated solid three times with absolute ethanol and then conduct vacuum drying. The drying temperature is 45 - 55 °C. After drying for 8 - 12 hours, obtain the nano-zinc oxide intermediate and grind it into a powder for standby;

[0057] S15. Dissolve tetraphenylporphyrin in N,N-dimethylformamide under stirring. After stirring for 1 - 2 hours, slowly add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide. Raise the temperature to 45 - 60 °C and continue stirring for 4 - 6 hours to obtain the third mixed solution;

[0058] S16. Under light avoidance and nitrogen protection, disperse the nano-zinc oxide intermediate in N,N-dimethylformamide. After stirring for 0.5 - 1 hour, slowly add the third mixed solution and continue stirring at room temperature for 24 - 28 hours to obtain the fourth mixed solution;

[0059] S17. Centrifuge the fourth mixed solution at a high speed at a rotation speed of 10000 - 12000 rpm for 15 - 20 minutes. Wash the separated solid three times alternately with N,N-dimethylformamide and absolute ethanol and then conduct vacuum drying. The drying temperature is 40 - 50 °C. After drying for 12 - 14 hours, obtain the modified nano-zinc oxide and grind it into a powder for standby.

[0060] The mass ratio of zinc acetate to tetraphenylporphyrin is 1:(0.01 - 0.1).

[0061] The mass ratio of tetraphenylporphyrin, N,N-dimethylformamide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide is 1:(2 - 4):(0.5 - 1):(0.5 - 1).

[0062] The preparation method of the modified nano-hydroxyapatite includes:

[0063] S21. Dissolve calcium dihydrogen phosphate and calcium hydroxide in purified water under stirring. After stirring for 0.5 - 1 hour, slowly add cetyltrimethylammonium bromide, and at the same time adjust the pH value to 10.5 - 11 with ammonia water solution. Continue stirring for 1 - 2 hours to obtain the fifth mixed solution;

[0064] S22. Transfer the fifth mixed solution to a hydrothermal reaction kettle for hydrothermal reaction. The reaction temperature is 180 - 200 °C. After reacting for 12 - 15 hours, obtain the sixth mixed solution;

[0065] S23. Centrifuge the sixth mixed solution at a high speed at a rotation speed of 10,000 - 12,000 rpm for 10 - 15 minutes. Wash the separated solid alternately with purified water and absolute ethanol three times, then perform freeze-drying. The drying temperature is -40 to -60 °C. After drying for 20 - 24 hours, obtain rod-shaped nano-hydroxyapatite and grind it into a powder for standby;

[0066] S24. Dissolve sodium fluoride in purified water under stirring to obtain a sodium fluoride solution. Disperse the rod-shaped nano-hydroxyapatite and γ-methacryloyloxypropyltrimethoxysilane in purified water and perform ultrasonic treatment. The ultrasonic frequency is 40 - 50 kHz. After ultrasonic treatment at room temperature for 0.5 - 1 hour, slowly add the sodium fluoride solution, control the dropping rate at 1 - 2 mL / min, and continue stirring at room temperature for 4 - 6 hours to obtain the seventh mixed solution;

[0067] S25. Centrifuge the seventh mixed solution at a high speed at a rotation speed of 10,000 - 12,000 rpm for 15 - 20 minutes. Wash the separated solid with purified water five times, then perform freeze-drying. The drying temperature is -40 to -60 °C. After drying for 20 - 24 hours, obtain modified nano-hydroxyapatite and grind it into a powder for standby.

[0068] The modified nano-hydroxyapatite is obtained by surface modification with sodium fluoride, and its shape is rod-shaped. The particle size length is 60 - 80 nm, the diameter is 15 - 25 nm, and the specific surface area is 180 - 250 m 2 / g.

[0069] The mass ratio of calcium dihydrogen phosphate, calcium hydroxide, and sodium fluoride is (1 - 1.5):(1.5 - 2):(0.02 - 0.1).

[0070] A preparation method of a tooth polishing agent with antibacterial efficacy, applied to the tooth polishing agent with antibacterial efficacy described above, includes the following steps:

[0071] S31. Add purified water to a container, and successively add sodium carboxymethylcellulose, glycerol, xanthan gum, and propylene glycol and stir. At the same time, perform water bath heating, and the heating temperature is 70 - 85 °C until it is fully dissolved to obtain the eighth mixed solution;

[0072] S32. Transfer the eighth mixed solution to a vacuum emulsifier, then slowly add cocamidopropyl betaine, emulsify for 5 - 10 minutes, and then slowly add silica, modified nano-zinc oxide, modified nano-hydroxyapatite, glucono delta-lactone, stevioside, and sodium benzoate. The stirring speed is 2000 - 4000 rpm, and continue emulsifying for 25 - 30 minutes to obtain the ninth mixed solution;

[0073] S33. Vacuum degas the ninth mixed solution, set the pressure at -0.05 to -0.08 MPa, and after degassing for 15 to 20 minutes, a uniform and delicate paste is obtained, which is the tooth polishing agent.

[0074] The viscosity of the tooth polishing agent is 20,000 to 30,000 mPa·s, and the specific gravity is 1.2 to 1.5 g / cm 3 and the pH value is between 6.8 and 7.2.

[0075] Example 2: This example discloses a tooth polishing agent with antibacterial efficacy, which includes the following raw materials in parts by weight: 12 parts of silica, 4 parts of sodium carboxymethyl cellulose, 2 parts of xanthan gum, 2 parts of propylene glycol, 4 parts of cocamidopropyl betaine, 6 parts of glycerol, 1 part of modified nano-hydroxyapatite, 2 parts of glucono delta-lactone, 2 parts of stevioside, 4 parts of sodium benzoate. The tooth polishing agent with antibacterial efficacy also includes modified nano-zinc oxide. The weight ratio of the modified nano-zinc oxide to silica is 1:12. The modified nano-zinc oxide is obtained by surface coupling with tetraphenylporphyrin, and the particle size of the modified nano-zinc oxide is 40 to 60 nm, and the specific surface area is 120 to 150 m 2 / g. The preparation methods of the modified nano-zinc oxide and the modified nano-hydroxyapatite in this example are the same as those in Example 1. The preparation method of the tooth polishing agent with antibacterial efficacy in this example is the same as that in Example 1.

[0076] Example 3: This example discloses a tooth polishing agent with antibacterial efficacy, which includes the following raw materials in parts by weight: 8 parts of silica, 2 parts of sodium carboxymethyl cellulose, 1 part of xanthan gum, 1 part of propylene glycol, 2 parts of cocamidopropyl betaine, 4 parts of glycerol, 0.5 part of modified nano-hydroxyapatite, 1 part of glucono delta-lactone, 1 part of stevioside, 2 parts of sodium benzoate. The tooth polishing agent with antibacterial efficacy also includes modified nano-zinc oxide. The weight ratio of the modified nano-zinc oxide to silica is 1:8. The modified nano-zinc oxide is obtained by surface coupling with tetraphenylporphyrin, and the particle size of the modified nano-zinc oxide is 40 to 60 nm, and the specific surface area is 120 to 150 m 2 / g. The preparation methods of the modified nano-zinc oxide and the modified nano-hydroxyapatite in this example are the same as those in Example 1. The preparation method of the tooth polishing agent with antibacterial efficacy in this example is the same as that in Example 1.

[0077] Control Group 1: The difference between this example and Example 1 is that it does not contain modified nano-zinc oxide. This example discloses a tooth polishing agent with antibacterial efficacy, which comprises the following raw materials in parts by weight: 10 parts of silicon dioxide, 3 parts of sodium carboxymethyl cellulose, 1.5 parts of xanthan gum, 1.5 parts of propylene glycol, 3 parts of cocamidopropyl betaine, 5 parts of glycerol, 0.8 part of modified nano-hydroxyapatite, 1.5 parts of glucono delta-lactone, 1.5 parts of stevioside, and 3 parts of sodium benzoate. The preparation method of the modified nano-hydroxyapatite in this example is the same as that in Example 1. The preparation method of a tooth polishing agent with antibacterial efficacy in this example is the same as that in Example 1.

[0078] Control Group 2: The difference between this example and Example 1 is that it does not contain modified nano-hydroxyapatite. This example discloses a tooth polishing agent with antibacterial efficacy, which comprises the following raw materials in parts by weight: 10 parts of silicon dioxide, 3 parts of sodium carboxymethyl cellulose, 1.5 parts of xanthan gum, 1.5 parts of propylene glycol, 3 parts of cocamidopropyl betaine, 5 parts of glycerol, 1.5 parts of glucono delta-lactone, 1.5 parts of stevioside, and 3 parts of sodium benzoate. The tooth polishing agent with antibacterial efficacy further comprises modified nano-zinc oxide. The weight ratio of the modified nano-zinc oxide to silicon dioxide is 1:10. The modified nano-zinc oxide is obtained by surface coupling with tetraphenylporphyrin, and the particle size of the modified nano-zinc oxide is 40 - 60 nm, and the specific surface area is 120 - 150 m 2 / g. The preparation method of the modified nano-zinc oxide in this example is the same as that in Example 1. The preparation method of a tooth polishing agent with antibacterial efficacy in this example is the same as that in Example 1.

[0079] Control Group 3: The difference between this example and Example 1 is that it does not contain glucono delta-lactone. It comprises the following raw materials in parts by weight: 10 parts of silicon dioxide, 3 parts of sodium carboxymethyl cellulose, 1.5 parts of xanthan gum, 1.5 parts of propylene glycol, 3 parts of cocamidopropyl betaine, 5 parts of glycerol, 0.8 part of modified nano-hydroxyapatite, 1.5 parts of stevioside, and 3 parts of sodium benzoate. The tooth polishing agent with antibacterial efficacy further comprises modified nano-zinc oxide. The weight ratio of the modified nano-zinc oxide to silicon dioxide is 1:10. The modified nano-zinc oxide is obtained by surface coupling with tetraphenylporphyrin, and the particle size of the modified nano-zinc oxide is 40 - 60 nm, and the specific surface area is 120 - 150 m 2 / g. The preparation methods of the modified nano-zinc oxide and modified nano-hydroxyapatite in this example are the same as those in Example 1. The preparation method of a tooth polishing agent with antibacterial efficacy in this example is the same as that in Example 1.

[0080] Effect evaluation: In vitro antibacterial experiment: Five common oral bacterial species, namely Streptococcus mutans, Porphyromonas gingivalis, Candida boidinii, Lactococcus lactis, and Enterococcus faecalis, were selected. All the bacterial species were purchased from the China Center for Type Culture Collection. The specific experimental methods are as follows: (1) Preparation of bacterial suspension: The bacterial species were inoculated on a slant nutrient medium and cultured at 37°C for 6 - 8 h. Then, the bacteria were resuspended with sterile normal saline, and the concentration was adjusted to 1.5×10 8 CFU / mL for standby; (2) Inoculation of bacteria: A sterile cotton swab was dipped into the bacterial suspension with adjusted turbidity and evenly spread on the agar surface three times. Each time, the plate was rotated 60° to ensure uniform distribution of the bacteria. After standing for 5 minutes, the bacterial suspension was absorbed by the agar; (3) Testing of tooth polishing agent samples: Sterile dry filter paper was punched with a hole punch to a diameter of 6 mm and placed in the tooth polishing agent prepared in each experimental group to ensure full impregnation. Another sterile dry filter paper was also soaked in sterile normal saline as a blank control. The filter paper soaked with the tooth polishing agent was placed in the center of the agar plate inoculated with bacteria, and the filter paper was gently pressed to ensure full contact with the agar surface; (4) Cultivation: The plates were inverted and placed in an incubator at 37°C. Aerobic bacteria such as Streptococcus mutans, Candida boidinii, Lactococcus lactis, and Enterococcus faecalis were cultured in an aerobic incubator for 24 - 48 h, and anaerobic bacteria such as Porphyromonas gingivalis were cultured in an anaerobic incubator for 48 - 72 h; (5) Measurement of the inhibition zone: After the cultivation was completed, the plates were taken out, and the diameter of the inhibition zone was measured with a vernier caliper or a ruler, accurate to 0.1 mm. Each plate was measured three times, and the average value was taken.

[0081] Table 1 Statistical table of the size of the inhibition zone of tooth polishing agent (mm)

[0082] Group Streptococcus mutans Porphyromonas gingivalis Candida boidinii Lactococcus lactis Enterococcus faecalis Example 1 15.8 14.2 1 5.7 16.2 1 5.4 Example 2 15.2 14.0 1 5.5 16.2 1 5.2 Example 3 14.5 14.0 15.0 15.5 14.4 Control Group 1 11.4 10.2 12.4 11.6 12.2 Control Group 2 13.6 12.9 1 3.8 1 3.5 13.9 Control Group 3 8.9 10.4 11.2 10.6 9.8 Blank Group 4.3 2.1 5.6 4.4 3.7

[0083] Table 1 shows the statistical results of the measurement of the size of the inhibition zone of the tooth polishing agent in each experimental group. It can be seen from Table 1 that there are significant differences in the antibacterial efficacy of the tooth polishing agents prepared in each experimental group. The blank group showed no antibacterial effect. By comparing the antibacterial effects of the tooth polishing agents prepared in Examples 1 - 3 and Controls 1 - 3, it can be found that overall, the tooth polishing agents prepared in Examples 1 - 3 have better antibacterial effects. The diameter of the inhibition zone is between 14 - 17 mm, showing a strong antibacterial effect. Moreover, the diameter of the inhibition zone of the tooth polishing agent prepared in Example 1 is larger than that of Examples 2 and 3, indicating that the tooth polishing agent prepared in Example 1 has the best antibacterial effect. The diameter of the inhibition zone of the tooth polishing agents prepared in Controls 1 - 3 is ≤14 mm, showing a weak antibacterial effect. This shows that by adding three substances, namely modified nano-zinc oxide, modified nano-hydroxyapatite, and gluconolactone, simultaneously during the preparation of the tooth polishing agent, a tooth polishing agent with excellent antibacterial efficacy can be obtained.

[0084] After the above limited experiments, the application effect of a tooth polishing agent with antibacterial efficacy in Example 1 of the present invention is remarkable. By adding three substances, namely modified nano-zinc oxide, glucono delta-lactone and modified nano-hydroxyapatite, during the production process, the antibacterial efficacy of the tooth polishing agent can be significantly improved, and a more persistent and effective antibacterial environment can be provided for the oral cavity. The three form a dynamic balance system, which can further strengthen the tooth structure by repairing the tiny defects of tooth enamel while ensuring the polishing effect, enhance the tooth strength, adjust the pH value to maintain the oral environment, effectively prevent oral diseases, and provide comprehensive and long-term protection for oral health.

[0085] Finally, it should be noted that although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A tooth polishing agent with antibacterial effect, characterized in that: The invention comprises the following raw materials in parts by weight: 8 to 12 parts of silicon dioxide, 2 to 4 parts of sodium carboxymethyl cellulose, 1 to 2 parts of xanthan gum, 1 to 2 parts of propylene glycol, 2 to 4 parts of cocamidopropyl betaine, 4 to 6 parts of glycerol, 0.5 to 1 part of modified nano-hydroxyapatite, 1 to 2 parts of gluconolactone, 1 to 2 parts of steviol glycoside, and 2 to 4 parts of sodium benzoate; The tooth polishing agent with antibacterial effect also includes: Modified nano zinc oxide; The modified nano zinc oxide and silicon dioxide are in a weight ratio of 1:(8-12); The modified nano zinc oxide is obtained by surface coupling tetraphenylporphyrin, and the particle size of the modified nano zinc oxide is 40-60nm, and the specific surface area is 120-150m 2 / g; The preparation method of the modified nano zinc oxide comprises: S11. Dissolve zinc acetate in purified water under stirring, stir for 0.5 to 1 h, then slowly add 0.1 mol / L sodium hydroxide solution, raise the temperature to 55 to 65 ° C, continue stirring and react for 4 to 6 h to obtain a first mixed solution; S12. The first mixed solution was subjected to high-speed centrifugation at a speed of 6000 to 8000 rpm for 10 to 15 min, and the separated solid was washed three times with purified water and then vacuum dried at a drying temperature of 50 to 60 ° C. After drying for 12 to 14 h, zinc oxide was obtained and ground into powder for standby use; S13. Dispersing zinc oxide in anhydrous ethanol under nitrogen protection and subjecting to ultrasonic treatment at an ultrasonic frequency of 40 to 50 kHz, subjecting the zinc oxide to ultrasonic treatment at room temperature for 0.5 to 1 h, slowly adding 3-aminopropyltriethoxysilane and subjecting the mixture to reflux at a reflux temperature of 75 to 80 ° C., and subjecting the mixture to reflux for 6 to 8 h to obtain a second mixed solution; S14. The second mixed solution was subjected to high-speed centrifugation at a speed of 8000 to 10000 rpm for 15 to 20 min. The separated solid was washed three times with anhydrous ethanol and then vacuum dried at a drying temperature of 45 to 55 ° C. After drying for 8 to 12 h, the nano zinc oxide intermediate was ground into a powder for standby use; S15. Dissolve tetraphenylporphyrin in N,N-dimethylformamide under stirring, slowly add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide after stirring for 1 to 2 hours, raise the temperature to 45 to 60° C., continue stirring for 4 to 6 hours to obtain a third mixed solution; S16. Disperse the nano zinc oxide intermediate in N,N-dimethylformamide under light protection and nitrogen protection, slowly add the third mixed solution after stirring for 0.5 to 1 hour, and continue stirring at room temperature for 24 to 28 hours to obtain a fourth mixed solution; S17. The fourth mixed solution is subjected to high-speed centrifugation at a rotation speed of 10000-12000 rpm for 15-20 min. The separated solid is washed alternately with N,N-dimethylformamide and anhydrous ethanol for 3 times and then vacuum dried at a drying temperature of 40-50° C. After drying for 12-14 h, modified nano zinc oxide is obtained and ground into powder for later use.

2. A tooth polishing agent with antibacterial effect according to claim 1, characterized in that: The mass ratio of the zinc acetate to tetraphenylporphyrin is 1:(0.01-0.1).

3. The tooth polishing agent with antibacterial effect according to claim 1, characterized in that: The mass ratio of tetraphenylporphyrin, N,N-dimethylformamide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide is 1:(2-4):(0.5-1):(0.5-1).

4. The tooth polishing agent with antibacterial effect according to claim 1, characterized in that: The preparation method of the modified nano-hydroxyapatite comprises: S21. Dissolve calcium dihydrogen phosphate and calcium hydroxide in purified water under stirring, slowly add hexadecyltrimethylammonium bromide after stirring for 0.5 to 1 h, and adjust the pH value to 10.5 to 11 with an ammonia solution, and continue stirring for 1 to 2 h to obtain a fifth mixed solution; S22. The fifth mixed solution is transferred to a hydrothermal reactor for hydrothermal reaction at a temperature of 180 to 200 ° C. and reacted for 12 to 15 hours to obtain a sixth mixed solution; S23. The sixth mixed solution is subjected to high-speed centrifugation at a speed of 10000 to 12000 rpm for 10 to 15 min, and the separated solid is washed three times with purified water and anhydrous ethanol alternately and then freeze-dried at a drying temperature of -40 to -60 ° C. After drying for 20 to 24 h, rod-shaped nano-hydroxyapatite is obtained and ground into powder for standby use; S24. Dissolve sodium fluoride in purified water under stirring to obtain a sodium fluoride solution, disperse rod-shaped nanohydroxyapatite and γ-methacryloxypropyltrimethoxysilane in purified water and perform ultrasonic treatment at an ultrasonic frequency of 40 to 50 kHz. After ultrasonic treatment at room temperature for 0.5 to 1 h, slowly add the sodium fluoride solution, control the drop rate to 1 to 2 mL / min, and continue stirring at room temperature for 4 to 6 h to obtain a seventh mixed solution; S25. The seventh mixed solution is subjected to high-speed centrifugation at a rotation speed of 10,000 to 12,000 rpm for 15 to 20 minutes. The separated solid is washed 5 times with purified water and then freeze-dried at a drying temperature of -40 to -60°C. After drying for 20 to 24 hours, the modified nano-hydroxyapatite is obtained and ground into powder for later use.

5. A tooth polishing agent with antibacterial effect according to claim 4, characterized in that: The modified nano-hydroxyapatite is obtained by surface modification of sodium fluoride and is rod-shaped with a particle length of 60 to 80 nm, a diameter of 15 to 25 nm, and a specific surface area of ​​180 to 250 m 2 / g.

6. The tooth polishing agent with antibacterial effect according to claim 4, characterized in that: The mass ratio of the monocalcium phosphate, calcium hydroxide and sodium fluoride is (1-1.5):(1.5-2):(0.02-0.1).

7. A method for preparing a tooth polishing agent with antibacterial effect, which is used for preparing a tooth polishing agent with antibacterial effect as claimed in any one of claims 1 to 6, characterized in that: The method comprises the following steps: S31. Purified water was added to the container, followed by sodium carboxymethyl cellulose, glycerol, xanthan gum and propylene glycol, and stirred while heating in a water bath at a temperature of 70 to 85 ° C until fully dissolved to obtain an eighth mixed solution; S32. The eighth mixed solution was transferred to a vacuum emulsifier, and then cocamidopropyl betaine was slowly added, and silica, modified nano zinc oxide, modified nano hydroxyapatite, gluconolactone, steviol glycosides and sodium benzoate were slowly added after emulsification for 5 to 10 min, and the stirring speed was 2000 to 4000 rpm, and the emulsification was continued for 25 to 30 min to obtain a ninth mixed solution; S33. The ninth mixed solution is subjected to vacuum degassing, and the pressure is set to -0.05 to -0.08 MPa. After degassing for 15 to 20 minutes, a uniform and fine paste is obtained, which is the tooth polishing agent.

8. The method for preparing a tooth polishing agent with antibacterial effect according to claim 7, characterized in that: The tooth polishing agent has a viscosity of 20,000 to 30,000 mPa·s and a specific gravity of 1.2 to 1.5 g / cm 3 And the pH value is between 6.8 and 7.2.

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