Medical sodium hyaluronate skin repair gel and preparation method thereof

Abstract

The invention discloses a medical sodium hyaluronate skin repair gel and a preparation method thereof, and mainly relates to the technical field of hyaluronic acid, the medical sodium hyaluronate skin repair gel comprises the following components: sodium hyaluronate, a synergist, glycerol, carbomer, 1, 3-butanediol, triethanolamine and water. Compared with the prior art, the medical sodium hyaluronate skin repair gel prepared by the invention can relieve erythema, pruritus and other symptoms caused by skin inflammation; the wound dressing can also be used for nursing non-chronic wounds, such as superficial wounds, sutured wounds after operations, mechanical wounds and the like; meanwhile, a bacteriostatic environment for wound healing is kept, and skin repair is accelerated; the scar hyperplasia phenomenon in the skin wound repairing process is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of hyaluronic acid, and particularly relates to a medical sodium hyaluronate skin repair gel and a preparation method thereof. Background Art

[0002] The medical sodium hyaluronate repair gel is a gel preparation mainly composed of sodium hyaluronate and added with various auxiliary components. Sodium hyaluronate has good moisturizing property, biocompatibility and tissue repair function. It can absorb and lock a large amount of moisture, provide a moist environment for skin cells, help maintain the normal morphology and function of cells, and promote skin metabolism. This repair gel is mainly applied in skin repair. For example, in dermatological clinical treatment, it can be used to treat skin injuries caused by burns, scalds, wounds, etc., promote wound healing, and reduce scar formation; in the field of cosmetic plastic surgery, it can be used for post-operative skin repair after laser treatment, microneedle treatment, chemical peeling, etc., relieve skin inflammatory reactions, and accelerate the recovery of skin barrier function; it can also be used for daily skin care, improve skin problems such as dryness and roughness, enhance the moisturizing ability and elasticity of the skin, and improve the overall health status of the skin.

[0003] Before using the medical sodium hyaluronate repair gel for skin repair, it is necessary to ensure that the hands are clean to prevent bacterial contamination of the wound. Bacterial infection will disrupt the normal wound healing process, trigger inflammatory reactions, etc., resulting in redness, swelling and increased pain of the wound, and prolonging the healing time. To avoid or slow down scar hyperplasia problems in the repaired wound, the risk of scar hyperplasia is usually avoided by accelerating the healing speed, reducing inflammation, reducing bacterial infection, paying attention to wound hygiene, controlling diet, etc. During the wound healing period, it is also necessary to avoid excessive tension on the wound, such as avoiding excessive activity or pulling the wound area, promoting wound healing and reducing scar formation, and maximizing skin repair and reducing the degree of scar hyperplasia.

[0004] CN114191597A discloses a liquid dressing for skin wound repair, its preparation method and uses. The liquid dressing for skin wound repair contains the following raw materials by weight percentage: 0.05 - 0.2% D-(+)-trehalose dihydrate, 0.05 - 0.2% hyaluronate, 1 - 5% 1,3-butanediol, 1 - 5% glycerol, and water up to 100%. The experimental results show that the liquid dressing prepared by the technical solution of the present invention is safe and non-irritating, can protect the skin wound from external pollutants and bacteria invasion, and at the same time play the role of moisturizing and repairing the wound skin.

[0005] CN114652664A discloses a repair gel and its preparation method, which comprises the following components: based on the total weight of the repair gel, 23%-36% of umbilical cord tissue extract, 5%-20% of humectant, 5%-20% of skin conditioner, and the balance of deionized water. The invention also provides a preparation method, which includes the following steps: stirring the humectant, umbilical cord tissue extract, skin conditioner and deionized water until uniform, and optionally adding a pH regulator to adjust the pH value to 5-7 to obtain the repair gel; or mixing the humectant with one or several of antioxidant, chelating agent and thickening agent, then heating the mixture to 50-85°C, stirring until uniform, and after cooling to room temperature, adding umbilical cord tissue extract, skin conditioner and deionized water and stirring until uniform, and optionally adding a pH regulator to adjust the pH value to 5-7 to obtain the repair gel. The repair gel of the invention not only has an anti-inflammatory effect on the application of scald wounds, but also can promote skin repair and regeneration.

[0006] The solutions reported in the above patent documents all solve either the problems of skin moisturization and repair, wound bacterial infection, or the problems of scalded skin repair and skin regeneration, etc., and cannot simultaneously solve the problems of skin inflammation, scalds, wound repair, etc., as well as wound bacterial infection problems, and the scar hyperplasia phenomenon caused by skin wounds during actual use. Summary of the Invention

[0007] In view of the above-mentioned defects of the prior art, the technical problems to be solved by the present invention are to relieve the symptoms of erythema, itching, stinging, fever, and dry desquamation caused by skin inflammation; it can also be used for the care of non-chronic wounds, including superficial wounds, postoperative suture wounds, mechanical traumas, small wounds, abrasions, incised wound surfaces, puncture sites of puncture instruments, first-degree or superficial second-degree burn and scald wounds, laser / photon / fruit acid peel / microplasty postoperative wounds; at the same time, maintain an antibacterial environment for wound healing, accelerate skin repair; reduce the scar hyperplasia phenomenon during the skin wound repair process.

[0008] To achieve the above object, the present invention provides a medical sodium hyaluronate skin repair gel and its preparation method.

[0009] The medical sodium hyaluronate skin repair gel comprises the following components: sodium hyaluronate, synergist, glycerol, carbomer, 1,3-butanediol, triethanolamine, water; preferably, it comprises the following components in parts by weight: 0.2-0.4 part of sodium hyaluronate, 0.1-0.2 part of synergist, 0.1-0.2 part of glycerol, 0.05-0.1 part of carbomer, 0.02-0.04 part of 1,3-butanediol, 0.02-0.04 part of triethanolamine, 15-20 parts of water.

[0010] The molecular weight of the sodium hyaluronate is 1.5-2.5 million.

[0011] The synergist is one of D-(+)-trehalose dihydrate and composite modified D-(+)-trehalose dihydrate hydrogel.

[0012] The preparation method of the composite modified D-(+)-trehalose dihydrate hydrogel is as follows:

[0013] Add 0.5 - 1 part of chitosan to 2 - 4 parts of water, dropwise add citric acid aqueous solution to adjust the pH to 2 - 4, stir at room temperature for 8 - 12 hours, add 0.1 - 0.2 part of D-(+)-trehalose dihydrate and 0.4 - 0.8 part of lysate of saccharomyces bispora fermentation product, continue to stir for 0.5 - 1 hour, add 0.2 - 0.6 part of oligosaccharide and continue to stir for 0.5 - 1.5 hours, dropwise add sodium citrate aqueous solution to adjust the pH to 6 - 7 to obtain the composite modified D-(+)-trehalose dihydrate hydrogel.

[0014] The citric acid aqueous solution is 1 - 2 wt% citric acid aqueous solution.

[0015] The sodium citrate aqueous solution is 1 - 2 wt% sodium citrate aqueous solution.

[0016] The oligosaccharide is fucoidan oligosaccharide.

[0017] The present invention also discloses a preparation method of a medical sodium hyaluronate skin repair gel as follows:

[0018] Add sodium hyaluronate, glycerol, carbomer, 1,3-butanediol, and triethanolamine into water and mix evenly, stir for 1 - 2 hours, cool to room temperature, add the synergist, and ultrasonically disperse for 5 - 15 minutes to obtain the medical sodium hyaluronate skin repair gel.

[0019] The stirring conditions for the 1 - 2 hours of stirring are stirring at 45 - 55 °C and 60 - 80 r / min for 1 - 2 hours.

[0020] Sodium hyaluronate has good moisturizing properties. It can form a moisturizing film on the skin surface to lock in moisture, and also improve the elasticity and softness of the skin, which plays an important role in moisturizing during wound repair and maintaining a good skin physiological environment. Glycerol is a commonly used moisturizer that can absorb moisture from the air and help keep the skin in a moist state, relieve the symptoms of dry skin and peeling, and at the same time make the gel texture more moisturizing, which helps with spreading and adhering to the skin surface. Carbomer plays a thickening role, enabling the components to mix well together to form a gel state, endowing the gel with appropriate viscosity and rheological properties, facilitating the use of the product on the skin, and helping the active ingredients to stay and slowly release on the skin surface. 1,3-Butanediol can help keep the skin hydrated, while promoting the dissolution and uniform mixing of other components, improving the stability and usability of the product. Triethanolamine is mainly used to adjust the pH value of the product to keep the gel system within a suitable pH range for the skin. Water serves as a solvent to provide a dispersion medium for other components, enabling the components to be fully and evenly mixed, and is the basis for forming the gel system.

[0021] The synergist is one of D-(+)-trehalose dihydrate and composite modified D-(+)-trehalose dihydrate hydrogel. The addition of D-(+)-trehalose dihydrate can reduce skin damage and scar hyperplasia when the skin shows symptoms such as erythema, itching, stinging, fever, dry desquamation, etc. caused by wounds, wounds, and inflammatory stimuli, prevent cells from being damaged due to changes in osmotic pressure, and then reduce abnormal cell stress responses and the release of inflammatory signals, ensure the relative stability of the cell metabolic pathway, and avoid excessive fibrosis caused by metabolic disorders, thereby reducing the possibility of scar tissue formation to a certain extent. In actual situations, the osmotic pressure of the local environment during the drug repair process of skin wounds may change, and both too high and too low osmotic pressures can damage cells. When the external osmotic pressure increases, D-(+)-trehalose dihydrate can combine with water molecules inside the cells to reduce the loss of intracellular water and prevent the cells from shrinking due to dehydration. When the external osmotic pressure decreases, it can also prevent the cells from swelling and bursting due to excessive water absorption. When the skin is traumatized or in an inflammatory environment, the water balance is disrupted, and the cells are easily damaged due to dehydration. D-(+)-trehalose dihydrate can replace water molecules to form hydrogen bonds with the phospholipid bilayer of the cell membrane, filling the vacancies left by the missing water molecules, thus preventing problems such as long skin repair cycles caused by the phase change, fusion, or rupture of the phospholipid molecules of the cell membrane due to the loss of water support.

[0022] The compound-modified D-(+)-trehalose dihydrate contains D-(+)-trehalose dihydrate, lysate of bifida ferment, fucoidan oligosaccharide, and chitosan. The carboxyl group of fucoidan oligosaccharide forms an amide bond with the amino group of chitosan, which can improve the gelation stability of the hydrogel compared with adding them separately. The obtained gel form encapsulates D-(+)-trehalose dihydrate and lysate of bifida ferment. The lysate of bifida ferment can provide various nutrients, promote the metabolism of skin cells, help repair damaged skin, and reduce scar hyperplasia. Fucoidan oligosaccharide has an anti-inflammatory effect and can relieve skin inflammatory reactions by inhibiting the release of inflammatory mediators, improving discomfort symptoms such as redness, swelling, and itching. Chitosan has good antibacterial properties, can inhibit the growth of bacteria at the wound surface, maintain an antibacterial environment for wound healing, reduce the risk of infection, and at the same time can promote wound healing and reduce scar hyperplasia. Through the synergistic effect of the components of the compound-modified D-(+)-trehalose dihydrate hydrogel, it plays a better role in relieving skin inflammatory symptoms, nursing non-chronic wounds, maintaining an antibacterial environment, and reducing scar hyperplasia.

[0023] Advantages of the present invention:

[0024] Compared with the prior art, the medical sodium hyaluronate skin repair gel of the present invention can relieve symptoms such as erythema, itching, stabbing pain, fever, and dry desquamation caused by skin inflammation; it can also be used for the nursing of non-chronic wounds, including superficial wounds, postoperative suture wounds, mechanical traumas, small wounds, abrasions, incised wound surfaces, puncture sites of puncture instruments, burn and scald wounds of degree I or superficial degree II, and wounds after laser / photon / fruit acid peel / microplasty; at the same time, it maintains an antibacterial environment for wound healing and accelerates skin repair. Specific embodiments

[0025] The parameters and sources of the specific chemical substances used in the examples are as follows:

[0026] Sodium hyaluronate: The molecular weight is 200 Da, and the manufacturer is Xi'an Zebang Biotechnology Co., Ltd.

[0027] D-(+)-trehalose dihydrate: The manufacturer is Hubei Weishi Chemical Reagent Co., Ltd., and the product number is HBWS-D344.

[0028] Lysate of bifida ferment: Brand: Repair Complex CLR TM PF, sourced from CLR Berlin.

[0029] Fucoidan oligosaccharide: The degree of polymerization is between 2 and 7, and the molecular weight is 400 - 1400 Da, sourced from Shanghai Chense Biochemical Co., Ltd.

[0030] Carbomer: Pharmaceutical grade, manufactured by Beijing Guoren Yikang Technology Co., Ltd., model Carbomer 940GE.

[0031] Example 1

[0032] A preparation method of a medical sodium hyaluronate skin repair gel is as follows:

[0033] Add 0.4g of sodium hyaluronate, 0.2g of glycerol, 0.08g of carbomer, 0.03g of 1,3 - butanediol, and 0.03g of triethanolamine to 18g of water, mix evenly, stir at 50°C and 70r / min for 1 hour, cool to room temperature, add 0.2g of D-(+)-trehalose dihydrate, and ultrasonically disperse for 10 minutes to obtain the medical sodium hyaluronate skin repair gel.

[0034] Example 2

[0035] A preparation method of a medical sodium hyaluronate skin repair gel is as follows:

[0036] Add 0.4g of sodium hyaluronate, 0.2g of glycerol, 0.08g of carbomer, 0.03g of 1,3 - butanediol, and 0.03g of triethanolamine to 18g of water, mix evenly, stir at 50°C and 70r / min for 1 hour, cool to room temperature, add 0.2g of composite modified D-(+)-trehalose dihydrate hydrogel, and ultrasonically disperse for 10 minutes to obtain the medical sodium hyaluronate skin repair gel.

[0037] The preparation method of the composite modified D-(+)-trehalose dihydrate hydrogel is as follows:

[0038] Add 0.6g of chitosan to 3g of water, dropwise add 1wt% citric acid aqueous solution to adjust the pH to 3, stir at room temperature for 10 hours, add 0.2g of D-(+)-trehalose dihydrate and 0.5g of lysate of Saccharomyces cerevisiae fermentation product, continue to stir for 0.5 hours, add 0.4g of fucoidan oligosaccharide, continue to stir for 1 hour, and dropwise add 1wt% sodium citrate aqueous solution to adjust the pH to 6.8 to obtain the composite modified D-(+)-trehalose dihydrate hydrogel.

[0039] Example 3

[0040] A preparation method of a medical sodium hyaluronate skin repair gel is as follows:

[0041] Add 0.4g of sodium hyaluronate, 0.2g of glycerol, 0.08g of carbomer, 0.03g of 1,3 - butanediol, and 0.03g of triethanolamine to 18g of water, mix evenly, stir at 50°C and 70r / min for 1 hour, cool to room temperature, add 0.2g of composite modified D-(+)-trehalose dihydrate hydrogel, and ultrasonically disperse for 10 minutes to obtain the medical sodium hyaluronate skin repair gel.

[0042] The preparation method of the composite modified D-(+)-trehalose dihydrate hydrogel is as follows:

[0043] Add 0.6 g of chitosan to 3 g of water, dropwise add 1 wt% citric acid aqueous solution to adjust the pH to 3, stir at room temperature for 10 hours, add 0.2 g of D-(+)-trehalose dihydrate and continue stirring for 0.5 hours, add 0.4 g of fucoidan oligosaccharide and continue stirring for 1 hour, dropwise add 1 wt% sodium citrate aqueous solution to adjust the pH to 6.8 to obtain the composite modified D-(+)-trehalose dihydrate hydrogel.

[0044] Example 4

[0045] The preparation method of a medical sodium hyaluronate skin repair gel is as follows:

[0046] Add 0.4 g of sodium hyaluronate, 0.2 g of glycerol, 0.08 g of carbomer, 0.03 g of 1,3-butanediol, and 0.03 g of triethanolamine to 18 g of water and mix evenly. Stir at 50 °C and 70 r / min for 1 hour, cool to room temperature, add 0.2 g of the composite modified D-(+)-trehalose dihydrate hydrogel, and ultrasonically disperse for 10 minutes to obtain the medical sodium hyaluronate skin repair gel.

[0047] The preparation method of the composite modified D-(+)-trehalose dihydrate hydrogel is as follows:

[0048] Add 0.6 g of chitosan to 3 g of water, dropwise add 1 wt% citric acid aqueous solution to adjust the pH to 3, stir at room temperature for 10 hours, add 0.5 g of lysate of Saccharomyces cerevisiae fermentate and continue stirring for 0.5 hours, dropwise add 1 wt% sodium citrate aqueous solution to adjust the pH to 6.8 to obtain the composite modified D-(+)-trehalose dihydrate hydrogel.

[0049] Example 5

[0050] The preparation method of a medical sodium hyaluronate skin repair gel is as follows:

[0051] Add 0.4 g of sodium hyaluronate, 0.2 g of glycerol, 0.08 g of carbomer, 0.03 g of 1,3-butanediol, and 0.03 g of triethanolamine to 18 g of water and mix evenly. Stir at 50 °C and 70 r / min for 1 hour, cool to room temperature, add 0.06 g of fucoidan oligosaccharide, 0.8 g of chitosan, 0.2 g of D-(+)-trehalose dihydrate, and 0.5 g of lysate of Saccharomyces cerevisiae fermentate, and ultrasonically disperse for 10 minutes to obtain the medical sodium hyaluronate skin repair gel.

[0052] Test Example 1

[0053] The medical sodium hyaluronate skin repair gel of Examples 1-5 was tested. 30 healthy experimental mice with a body weight of 20-25 g and half males and half females were selected and randomly divided into 6 groups, with 5 mice in each group, corresponding to the groups of Examples 1-5 and the control group. After anesthesia, hair removal was performed on the backs of the mice with a 6% sodium sulfide alcohol solution. The next day, after anesthetizing the mice with ether, a superficial second-degree wound with a diameter of 1.4 cm was uniformly created on the backs of the mice using a burn and scald instrument; the burned and scalded mice were all raised under SPF-level conditions; the groups of Examples 1-5 were treated by applying the medical sodium hyaluronate skin repair gel of Examples 1-5, twice a day for 7 days. The histological changes after wound healing were observed, including inflammatory response, angiogenesis, cell proliferation, etc., and the wound area was recorded on the 7th day (the wound surface was photographed and processed with Adobe Photoshop CS 6 software to calculate the wound surface area), and the wound area was recorded. The test data were averaged and summarized as shown in Table 1.

[0054] Table 1 Wound Area

[0055]

[0056] Note: Compared with the control group, there were statistically significant differences in Examples 1-5 (P<0.05); compared with Examples 1, 3-5, there were statistically significant differences in Example 2 (P<0.05).

[0057] The basic formulation of the Example 1 group contains sodium hyaluronate, glycerin, carbomer, 1,3-butanediol, triethanolamine, and D-(+)-trehalose dihydrate. Compared with the control group without intervention, the wound heals faster. Compared with Example 1, the wound healing speed of Example 2 is significantly increased and the inflammatory response is significantly reduced. The difference is that Example 2 performs composite modification on D-(+)-trehalose dihydrate; the composite modified D-(+)-trehalose dihydrate hydrogel inhibits scar hyperplasia from multiple levels. The bifidobacterium ferment lysate therein provides nutrients to promote the proliferation and differentiation of normal cells and reduces abnormal repair cell behavior caused by injury; fucoidan inhibits the inflammatory response, reduces the stimulation of fibroblasts by inflammatory factors, and reduces excessive collagen synthesis; chitosan regulates the metabolism of the extracellular matrix, optimizes the balance of collagen synthesis and degradation, and makes the arrangement of collagen fibers more orderly. At the same time, each component synergistically acts on the relevant signaling pathways in cells, such as regulating the activity of key fibrotic signaling pathways such as TGF-β, inhibiting the excessive activation of fibroblasts and the fibrotic process, thereby effectively reducing the risk of scar hyperplasia, promoting high-quality healing of skin wounds, and reducing the adverse effects of scars on the appearance and function of the skin. Example 3-4 cancel the single variables of bifidobacterium ferment lysate and fucoidan respectively on the basis of Example 2. In Example 5, the bifidobacterium ferment lysate, fucoidan, chitosan, and D-(+)-trehalose dihydrate of the composite modified D-(+)-trehalose dihydrate hydrogel in Example 2 are directly added to the preparation step without mixing and compounding. Although the Example 3 group lacks bifidobacterium ferment lysate, fucoidan and chitosan can still inhibit the inflammatory response, reduce excessive collagen synthesis, and regulate the metabolism of the extracellular matrix, thereby inhibiting scar hyperplasia in the early stage; Example 4 lacks fucoidan and has a relatively strong inflammatory response, but chitosan and the lysate of bifidobacterium ferment lysate still have a certain promoting effect on repair; the separate addition in Example 5 may result in slightly lower effects than Example 2 due to uneven compatibility and dispersibility.

[0058] Test Example 2

[0059] The in vitro antibacterial test was carried out on the medical sodium hyaluronate skin repair gel of Examples 1-5. The test was based on "Gel Agents" in the second part of the Chinese Pharmacopoeia; the bacterial suspensions with concentrations of 105-106 CFU / mL were prepared respectively in the test, and the test strains were Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, and Escherichia coli ATCC 8739. In the test, 100 cfu of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli were inoculated into the casein soya bean digest agar medium respectively. Two plates were prepared in parallel for each test strain, mixed evenly, solidified, and cultured at 30 °C for 24 hours. After elution with 0.9% sterile sodium chloride solution, the number of spores per 1 mL was 10 8For the spore suspension of cfu, the number of bacteria contained in 1 mL of the bacterial suspension was measured. If the prepared bacterial suspension was placed at room temperature, it should be used within 2 hours. 1 g of the medical sodium hyaluronate skin repair gel of Examples 1-5 was respectively applied to the center of a sterile container, and a test bacterial strain with a concentration of 10 5 -10 6 CFU / mL was inoculated, and they were fully mixed to make the test bacteria evenly distributed, and then stored in the dark at 20-25 °C. Counting was carried out at the 24th h and the reduced lg value was calculated. The "reduced lg value" refers to the difference value between the lg value of the number of bacteria measured at each interval time and the lg value of the number of bacteria inoculated in 1 mL of the example. According to the determination results of the viable bacteria count, the number of bacteria added to each test bacterium in 1 mL of the example and the number of bacteria at each interval time were calculated, and they were converted into lg values, which were summarized as shown in Table 2.

[0060] Table 2 In vitro antibacterial test data

[0061]

[0062] As can be seen from the above table, Example 2 is based on Example 1 and conducts composite modification on D-(+)-trehalose dihydrate, which is manifested as the best antibacterial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The main reason lies in the synergistic effect of various components in its composite modified D-(+)-trehalose dihydrate hydrogel. The bifida ferment lysate may contain antibacterial active substances that can directly inhibit the growth of bacteria. At the same time, the nutrients it provides can promote the growth of beneficial microorganisms and competitively inhibit harmful bacteria, thus enhancing the overall antibacterial ability. Alginate oligosaccharide has certain antibacterial activity and may play an antibacterial role by destroying the bacterial cell membrane structure or interfering with bacterial metabolism. And its property of inhibiting inflammatory response also helps to reduce the negative impact of inflammation caused by bacterial infection on the antibacterial effect. Chitosan itself is a natural antibacterial agent that can adsorb on the surface of bacteria, change the permeability of the bacterial cell membrane, and cause the leakage of bacterial contents and death. When acting synergistically with other components, it further enhances the inhibitory effect on the test strains. Compared with Example 2, Example 3 lacks bifida ferment lysate, and its antibacterial effect decreases, probably because the lack of antibacterial active substances and the regulatory effect on the microbial community provided by bifida ferment lysate make the inhibitory ability against each test bacterium inferior to that of Example 2. Example 4 lacks alginate oligosaccharide. Although other components still have antibacterial effects, the absence of alginate oligosaccharide leads to the impairment of its antibacterial auxiliary mechanisms related to destroying bacterial structure and inhibiting inflammation. Therefore, its antibacterial effect is similar to that of Example 3 and slightly weaker than that of Example 2. Example 5 does not carry out composite modification, and the synergistic effect between components is not as efficient as that after composite modification in Example 2. Although individual components also have certain antibacterial ability, the comprehensive antibacterial effect is lower than that of Example 2. As the basic formula, Example 1 only adds a single D-(+)-trehalose dihydrate, and its antibacterial ability is relatively weak because it lacks the synergistic enhancement of other active components and cannot effectively inhibit the test strains from multiple targets and mechanisms like Example 2, so its antibacterial effect is significantly lower than that of Example 2 and some other examples.

[0063] The preferred specific embodiments of the present invention have been described in detail above. It should be understood that those of ordinary skill in the art can make many modifications and variations based on the concept of the present invention without creative work. Therefore, all technical solutions that can be obtained by those skilled in the art in the technical field of the present invention based on the concept of the present invention through logical analysis, reasoning or limited experiments on the basis of the prior art shall fall within the protection scope determined by the claims.

Claims

1. A medical sodium hyaluronate skin repair gel, characterized in that: The invention comprises the following components in parts by weight: 0.2-0.4 parts of sodium hyaluronate, 0.1-0.2 parts of synergist, 0.1-0.2 parts of glycerin, 0.05-0.1 parts of carbomer, 0.02-0.04 parts of 1,3-butylene glycol, 0.02-0.04 parts of triethanolamine and 15-20 parts of water.

2. The medical sodium hyaluronate skin repairing gel according to claim 1, characterized in that: The molecular weight of the sodium hyaluronate is 1.5-2.5 million.

3. The medical sodium hyaluronate skin repairing gel according to claim 1, characterized in that: The synergist is one of D-(+)-trehalose dihydrate and composite modified D-(+)-trehalose dihydrate hydrogel.

4. The medical sodium hyaluronate skin repairing gel according to claim 3, characterized in that: The preparation method of the composite modified D-(+)-trehalose dihydrate hydrogel is as follows: 0.5-1 parts of chitosan are added to 2-4 parts of water, and a citric acid aqueous solution is added dropwise to adjust the pH to 2-4, and the mixture is stirred at room temperature for 8-12 hours. 0.1-0.2 parts of D-(+)-trehalose dihydrate and 0.4-0.8 parts of bifid yeast fermentation product lysate are added, and stirring is continued for 0.5-1 hour. 0.2-0.6 parts of oligosaccharides are added, and stirring is continued for 0.5-1.5 hours. A sodium citrate aqueous solution is added dropwise to adjust the pH to 6-7, and the composite modified D-(+)-trehalose dihydrate hydrogel is obtained.

5. The medical sodium hyaluronate skin repairing gel according to claim 4, characterized in that: The oligosaccharide is brown algae oligosaccharide.

6. A method for preparing the medical sodium hyaluronate skin repairing gel according to any one of claims 1 to 5, characterized in that: The preparation method is as follows: Sodium hyaluronate, glycerin, carbomer, 1,3-butylene glycol and triethanolamine are added into water and mixed evenly, stirred for 1-2 hours, cooled to room temperature, added with a synergist, and ultrasonically dispersed for 5-15 minutes to obtain the medical sodium hyaluronate skin repair gel.

7. The method for preparing the medical sodium hyaluronate skin repairing gel according to claim 6, characterized in that: The stirring conditions for 1-2 hours are stirring at 45-55° C. and 60-80 r / min for 1-2 hours.

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