Reutilization method of erythritol yeast residues

Abstract

The invention belongs to the technical field of sugar alcohol preparation, and relates to an erythritol yeast residue recycling method which comprises the following steps: filtering fermentation liquor obtained after erythritol fermentation culture is finished through a ceramic membrane to obtain erythritol yeast residues, and drying and crushing the yeast residues to obtain mushroom residue powder; and adding the mushroom dreg solid powder into an erythritol fermentation culture medium according to the addition amount of 0.05-2% by mass to replace part of yeast powder. According to the method, a large amount of erythritol yeast residues which are difficult to treat are prepared into the mushroom residue powder, so that the storage volume of the erythritol yeast residues is reduced, the risk of system contamination is reduced, and meanwhile, the mushroom residue powder is used in a fermentation culture medium of the erythritol and is used as a fermentation auxiliary material of the erythritol to replace part of expensive yeast powder, so that the production cost of the erythritol is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of sugar alcohol preparation, and particularly relates to a method for recycling erythritol yeast residue. Background Art

[0002] The existing methods for processing erythritol yeast residue are mainly: 1. Using the residue as raw material or auxiliary material to make compound feed; 2. Using digestion equipment for harmless treatment; 3. Making organic soil for rice and mushroom cultivation. Such treatment methods have high processing costs and low subsequent added value. There is not much research on other application methods of erythritol fermentation yeast residue. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide a method for recycling erythritol yeast residues, wherein the erythritol yeast residues are made into residue powder, and then the residue solid powder is added to the erythritol fermentation medium to replace part of the yeast powder for use, thereby reducing the production cost of erythritol.

[0004] The present invention is achieved by providing a method for recycling erythritol yeast residue, comprising the following steps: Step 1: After the fermentation broth of erythritol fermentation culture is finished, it is filtered through a ceramic membrane to obtain erythritol yeast residue, and the yeast residue is dried and crushed to obtain yeast residue powder; Step 2: Add the solid powder of the fungus residue into the erythritol fermentation medium at a mass ratio of 0.05% to 2% to replace part of the yeast powder.

[0005] Furthermore, in step 2, the formula of the erythritol fermentation medium is: 20%~40% glucose, 0.3% yeast powder, 0.05%~2% fungus residue powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest is fixed to volume with water.

[0006] Compared with the prior art, the method for recycling erythritol yeast residue of the present invention prepares erythritol yeast residue into residue powder, and then adds the residue solid powder into erythritol fermentation medium to replace part of yeast powder for use. The present invention prepares a large amount of difficult-to-handle erythritol yeast residue into residue powder, which not only reduces the storage volume of erythritol yeast residue and reduces the risk of system contamination, but also is used in the fermentation medium of erythritol as an auxiliary material for erythritol fermentation, replaces part of the expensive yeast powder, and reduces the production cost of erythritol. DETAILED DESCRIPTION

[0007] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0008] A preferred embodiment of the method for recycling erythritol yeast residue of the present invention comprises the following steps: Step 1: After the fermentation culture of erythritol is completed, the fermentation liquid is filtered through a ceramic membrane to obtain erythritol yeast residue, and the yeast residue is dried and crushed to obtain residue powder.

[0009] Step 2: Add the solid powder of the fungus residue into the erythritol fermentation medium at a mass ratio of 0.05% to 2% to replace part of the yeast powder.

[0010] Specifically, in step 2, the formula of the erythritol fermentation medium is: 20%-40% glucose, 0.3% yeast powder, 0.05%-2% fungus residue powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest is fixed to volume with water.

[0011] The method for recycling erythritol yeast residues of the present invention is further described below by means of specific examples.

[0012] Example 1

[0013] The first embodiment of the method for recycling erythritol yeast residue of the present invention comprises the following steps: Step 11: After the fermentation culture of erythritol is completed, the fermentation liquid is filtered through a ceramic membrane to obtain erythritol yeast residue, and the yeast residue is dried and crushed to obtain residue powder.

[0014] Step 12, prepare erythritol fermentation medium according to the formula groups of the following experimental group 1, comparison group 1, and comparison group 2, respectively, Formula of experimental group 1: glucose 20%, yeast powder 0.3%, mushroom residue powder 0.05%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, ammonium citrate 0.5%, and the rest was fixed to volume with water; The formula of control group 1: glucose 20%, yeast powder 0.3%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, ammonium citrate 0.5%, and the rest was fixed to volume with water; The formula of control group 2 was as follows: 20% glucose, 0.6% yeast powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest was made up to volume with water.

[0015] The only difference between the formula of control group 1 and experimental group 1 is that the fungus residue powder in the formula of experimental group 1 is replaced by 0.05% water. The only difference between the formula of control group 2 and experimental group 1 is that the fungus residue powder in the formula of experimental group 1 is replaced by 0.3% yeast powder, so the yeast powder in the formula reaches 0.6%.

[0016] Step 13, the fermentation strains of erythritol were inoculated into the fermentation medium prepared in the above groups respectively, and cultured and fermented on a shaker at a temperature of 30° C. and a rotation speed of 150 rpm.

[0017] Three parallel samples were tested for each group according to the above method, namely EG1-01~EG1-03, CG1-01~CG1-03, and CG2-01~CG2-03. The fermentation time, conversion rate, and OD value of the fermentation liquid were recorded after the fermentation of each sample. 600 The results shown in Table 1 below were obtained.

[0018] Table 1 Fermentation time, conversion rate and OD of each group in Example 1 600最高值 result

[0019] The experimental data of each group in Example 1 are summarized to obtain the summary comparison table of Table 2.

[0020] Table 2 Comparison table of experimental data of each group in Example 1

[0021] Note: In Table 2, + indicates average performance, ++ indicates good performance, and +++ indicates excellent performance.

[0022] The data in Tables 1 and 2 above show that the fermentation performance of the experimental group 1 with the addition of a trace amount of fungus residue powder (0.05%) is the same as that of the comparison group 1 with only a small amount of yeast powder (0.3%) added, and the fermentation time and conversion rate performance cannot reach the effect of the comparison group 2 with the addition of a sufficient amount of yeast powder (0.6%).

[0023] Example 2

[0024] The second embodiment of the method for recycling erythritol yeast residue of the present invention comprises the following steps: Step 21: After the fermentation culture of erythritol is completed, the fermentation liquid is filtered through a ceramic membrane to obtain erythritol yeast residue, and the yeast residue is dried and crushed to obtain yeast residue powder.

[0025] Step 22, prepare erythritol fermentation medium according to the formula groups of the following experimental group 2, comparison group 3, and comparison group 4, respectively, Formula of experimental group 2: 40% glucose, 0.3% yeast powder, 1% fungus residue powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest was fixed to volume with water; The formula of control group 3: glucose 40%, yeast powder 0.3%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, ammonium citrate 0.5%, and the rest was fixed to volume with water; The formula of control group 4 was as follows: 40% glucose, 0.6% yeast powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest was fixed to volume with water.

[0026] The only difference between the formula of control group 3 and experimental group 2 is that the fungus residue powder in the formula of experimental group 2 is replaced by 1% water. The only difference between the formula of control group 4 and experimental group 2 is that the fungus residue powder in the formula of experimental group 2 is replaced by 0.3% yeast powder, so the yeast powder in the formula reaches 0.6%.

[0027] Step 23, the fermentation strains of erythritol are inoculated into the fermentation medium prepared in the above groups respectively, and culture and ferment on a shaker at a temperature of 30° C. and a rotation speed of 150 rpm.

[0028] Three parallel samples were tested for each group according to the above method, namely EG2-01~EG2-03, CG3-01~CG3-03, and CG4-01~CG4-03. The fermentation time, conversion rate, and OD value of the fermentation liquid were recorded after the fermentation of each sample. 600 The results shown in Table 3 below were obtained.

[0029] Table 3 Fermentation time, conversion rate and OD of each group in Example 2 600最高值 result

[0030] The experimental data of each group in Example 2 are summarized to obtain the summary comparison table of Table 4.

[0031] Table 4 Comparison table of experimental data of each group in Example 2

[0032] Note: In Table 4, + indicates average performance, ++ indicates good performance, and +++ indicates excellent performance.

[0033] The data in Tables 3 and 4 above show that the fermentation performance of the experimental group 2 with a small amount of fungus residue powder (1%) added is better than that of the blank comparison group 3 with only a small amount of yeast powder (0.3%) added, and the fermentation time performance is better than that of the comparison group 4 with sufficient yeast powder (0.6%) added, but the conversion rate is lower than that of the comparison group 4 with sufficient yeast powder (0.6%) added.

[0034] Example 3

[0035] The third embodiment of the method for recycling erythritol yeast residue of the present invention comprises the following steps: Step 31: After the fermentation broth of erythritol fermentation culture is finished, it is filtered through a ceramic membrane to obtain erythritol yeast residue, and the yeast residue is dried and crushed to obtain residue powder.

[0036] Step 32, prepare erythritol fermentation medium according to the formula groups of the following experimental group 3, comparison group 5, and comparison group 6, respectively, Formula of experimental group 3: glucose 20%, yeast powder 0.3%, mushroom residue powder 2%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, ammonium citrate 0.5%, and the rest was fixed to volume with water; The formula of control group 5: glucose 20%, yeast powder 0.3%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, ammonium citrate 0.5%, and the rest was fixed to volume with water; The formula of control group 6 was as follows: 20% glucose, 0.6% yeast powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest was made up to volume with water.

[0037] The only difference between the formula of control group 5 and experimental group 3 is that the fungus residue powder in the formula of experimental group 3 is replaced by 2% water. The only difference between the formula of control group 6 and experimental group 3 is that the fungus residue powder in the formula of experimental group 3 is replaced by 0.3% yeast powder, so the yeast powder in the formula reaches 0.6%.

[0038] Step 33, inoculate the fermentation strains of erythritol into the fermentation medium prepared in the above groups respectively, and culture and ferment on a shaker at a temperature of 30° C. and a rotation speed of 150 rpm.

[0039] Three parallel sample tests were carried out in each group according to the above method, namely EG3-01~EG3-03, CG5-01~CG5-03, and CG6-01~CG6-03. The fermentation time, conversion rate, and OD value of the fermentation liquid were recorded after the fermentation of each sample. 600 The results shown in Table 5 below were obtained.

[0040] Table 5 Fermentation time, conversion rate and OD of each group in Example 3 600最高值result

[0041] The experimental data of each group in Example 3 are summarized to obtain the summary comparison table in Table 6.

[0042] Table 6 Comparison of experimental data of each group in Example 3

[0043] Note: In Table 6, + indicates average performance, ++ indicates good performance, and +++ indicates excellent performance.

[0044] The data in Tables 5 and 6 above show that the fermentation performance of the experimental group 3 with a large amount of fungus residue powder (2%) added is better than that of the comparison group 5 with only a small amount of yeast powder (0.3%) added, and the fermentation time performance is close to that of the comparison group 6 with a sufficient amount of yeast powder (0.6%) added, but the conversion rate is lower than that of the comparison group 6 with a sufficient amount of yeast powder (0.6%) added.

[0045] The experimental data of Examples 1 to 3 above show that adding an appropriate amount of fungus residue powder can improve the fermentation time, conversion rate and OD 600 , proving that adding the fungus residue powder of the present invention into the erythritol fermentation medium can indeed replace the effect of part of the yeast powder and achieve the expected purpose.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for recycling erythritol yeast residue, characterized in that: The steps include: Step 1: After the fermentation broth of erythritol fermentation culture is finished, it is filtered through a ceramic membrane to obtain erythritol yeast residue, and the yeast residue is dried and crushed to obtain yeast residue powder; Step 2: Add the solid powder of the fungus residue into the erythritol fermentation medium at a mass ratio of 0.05% to 2% to replace part of the yeast powder.

2. The method for recycling erythritol yeast residue according to claim 1, characterized in that: In step 2, the formula of the erythritol fermentation medium is: 20%-40% glucose, 0.3% yeast powder, 0.05%-2% fungus residue powder, 0.05% magnesium sulfate, 0.05% dipotassium hydrogen phosphate, 0.5% ammonium citrate, and the rest is fixed to volume with water.

Images