Hair drug colloidal carbon detection kit and detection method thereof

Abstract

The invention discloses a colloidal carbon detection kit for hair drugs. The colloidal carbon detection kit comprises a kit body and a colloidal carbon immunochromatography test strip, the colloidal carbon immunochromatography test strip comprises a nitrocellulose membrane and a carbon pad, the nitrocellulose membrane is provided with a test area and a quality control area, the test area is coated with bovine serum albumin coupled drug synthetic antigens, the quality control area is coated with goat anti-rabbit IgG, and the carbon pad is embedded with colloidal carbon labeled drug monoclonal antibodies and colloidal carbon labeled rabbit IgG. The detection method of the hair drug colloidal carbon detection kit provided by the invention is simple to operate, carries out drug detection on hair, can obtain a detection result within 5 minutes, and is suitable for rapid drug screening.

Description

Technical Field

[0001] The invention belongs to the technical field of drug detection, and in particular relates to a hair drug colloidal carbon detection kit and a detection method thereof. Background Art

[0002] At present, the biological test samples for rapid screening of drug users mainly include urine, saliva and hair. Compared with urine and saliva, hair samples have the advantages of stable properties, convenient collection, easy storage, long detection time limit, wide application range and less chance of contamination. Drugs are metabolized slowly in hair, and the detection window period can be as long as half a year, which facilitates supervision of the sampling process and prevents the samples from being adulterated or replaced. However, the concentration of drugs and their metabolites in hair is significantly lower than that in urine. Taking methamphetamine as an example, the detection threshold of drugs and their metabolites in urine is 1000ng / mL, while the detection threshold of drugs and their metabolites in hair is 0.2ng / mg. Therefore, in order to detect trace amounts of drugs in hair, it is extremely necessary to use technologies with higher detection sensitivity and better anti-interference.

[0003] The colloidal carbon detection technology used in the present invention is a new in vitro diagnostic technology that has been developed in recent years and can replace colloidal gold. Compared with the colloidal gold method, the sensitivity can be improved by 1-2 orders of magnitude, and the detection accuracy can reach 0.5-2ng / ml. It has the advantages of strong specificity, high sensitivity (reaching 0.2ng / mg), long traceability time, etc., and can detect drug results in 3-5 minutes at the fastest. In addition, colloidal carbon, as a nanomaterial, has good biocompatibility after special activation treatment. As a marker, it surpasses colloidal gold in terms of stability, validity period, environmental protection, and low cost. Summary of the invention

[0004] In order to solve the deficiencies in the prior art, the present invention provides a hair drug colloidal carbon detection kit and a detection method thereof to solve the problems in the prior art.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A hair drug colloidal carbon detection kit, characterized in that it comprises a box body and a colloidal carbon immunochromatography test strip; the colloidal carbon immunochromatography test strip comprises a nitrocellulose membrane and a carbon pad, the nitrocellulose membrane is provided with a test area (T) and a quality control area (C), the test area (T) is coated with bovine serum albumin (BSA)-coupled drug synthetic antigen, the quality control area (C) is coated with goat anti-rabbit IgG, and the carbon pad embeds colloidal carbon-labeled drug monoclonal antibodies and colloidal carbon-labeled rabbit IgG.

[0007] Furthermore, the box body comprises an upper cover and a bottom plate, the bottom plate is a PVC bottom plate, and the upper cover is provided with a sample addition hole; the sample addition hole corresponds to the position of the sample pad.

[0008] A method for detecting hair drug colloidal carbon detection kit comprises the following steps:

[0009] Step 1: Take out some hair, cut it into pieces less than 3 cm with scissors, take no less than 20 mg of hair, add it to the lysis tube, put the lysis tube into the grinder and grind it (grind for 1-2 minutes);

[0010] Step 2: After grinding, take out the lysis tube, use a quantitative dropper to absorb the processed hair lysis solution, and drop 1-2 drops into the sample well of the reagent kit. Read the result within 5 minutes. The result read after 10 minutes is invalid.

[0011] The present invention is established by adopting colloidal carbon immunochromatography technology, and its advantages and beneficial effects are that the present invention has stronger anti-interference performance for samples, higher detection sensitivity, and better result repeatability; on the other hand, drug detection for hair can produce test results within 5 minutes, which solves the problem that large instruments cannot perform on-site and immediate detection, and provides strong support for on-site rapid screening. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a judgment diagram of the detection result of the present invention. DETAILED DESCRIPTION

[0013] The present invention discloses a hair drug colloidal carbon detection kit and a detection method thereof. The specific implementation manner of the present invention is further described below in conjunction with preferred embodiments.

[0014] Example 1

[0015] The present embodiment provides a ketamine hair detection kit, comprising a box body and a colloidal carbon immunochromatography test strip; the colloidal carbon immunochromatography test strip comprises a nitrocellulose membrane and a carbon pad, the nitrocellulose membrane is provided with a test area (T) and a quality control area (C), the test area (T) is coated with bovine serum albumin (BSA) coupled to a ketamine synthetic antigen, the quality control area (C) is coated with goat anti-rabbit IgG, and the carbon pad is embedded with anti-ketamine monoclonal antibodies labeled with colloidal carbon and rabbit IgG labeled with colloidal carbon.

[0016] Furthermore, the box body comprises an upper cover and a bottom plate, the bottom plate is a PVC bottom plate, and the upper cover is provided with a sample addition hole; the sample addition hole corresponds to the position of the sample pad.

[0017] Example 2

[0018] This embodiment provides a method for drug detection using the kit described in embodiment 1, comprising the following steps:

[0019] Step 1: Take out some hair and cut it into 3 cm pieces with scissors. Add 25 mg of hair into a lysis tube and put the lysis tube into a grinder to grind for 2 minutes.

[0020] Step 2: After grinding, take out the lysis tube, use a quantitative dropper to absorb the processed hair lysis solution, drop 2 drops into the sample well of the kit, and read the results after 4 minutes.

[0021] After the sample is added, the solution begins to flow through the nitrocellulose membrane to the absorbent paper. If the sample is a negative sample, the colloidal carbon will be enriched in the test area (T) and the quality control area (C) to form a black strip. If the sample is a positive sample, the colloidal carbon will not be enriched in the test area (T) to form a strip, but will be enriched in the quality control area (C) to form a black strip. For details, see the result judgment diagram of the hair drug detection kit ( Figure 1 ).

Claims

1. A hair drug colloidal carbon detection kit, characterized in that: It comprises a box body and a colloidal carbon immunochromatography test strip; the colloidal carbon immunochromatography test strip comprises a nitrocellulose membrane and a carbon pad, the nitrocellulose membrane is provided with a test area (T) and a quality control area (C), the test area (T) is coated with bovine serum albumin (BSA)-coupled drug synthetic antigen, the quality control area (C) is coated with goat anti-rabbit IgG, and the carbon pad is embedded with colloidal carbon-labeled drug monoclonal antibodies and colloidal carbon-labeled rabbit IgG.

2. The hair drug colloidal carbon detection kit according to claim 1, characterized in that: The box body comprises an upper cover and a bottom plate, wherein the bottom plate is a PVC bottom plate, and the upper cover is provided with a sample addition hole; the sample addition hole corresponds to the position of the sample pad.

3. A hair drug colloidal carbon detection kit detection method, characterized in that: The following steps are involved: Step 1: Take out some hair, cut it into pieces less than 3 cm with scissors, take no less than 20 mg of hair, add it to the lysis tube, put the lysis tube into the grinder and grind it (grind for 1-2 minutes); Step 2: After grinding, take out the lysis tube, use a quantitative dropper to absorb the processed hair lysis solution, and drop 1-2 drops into the sample well of the reagent kit. Read the result within 5 minutes. The result read after 10 minutes is invalid.

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