Application of rubber seed oil in preparation of anti-depression product

Abstract

The invention relates to the technical field of anti-depression products, in particular to application of rubber seed oil in preparation of the anti-depression product, and the anti-depression product takes the rubber seed oil as a unique active ingredient and can also comprise auxiliary materials. The invention creatively provides the treatment application of the rubber seed oil in relieving depression, and mice experiments prove that the rubber seed oil can effectively relieve depression symptoms and improve the life quality of patients, so that a safer and more effective treatment scheme is provided for the patients with depression.

Description

Technical Field

[0001] The invention relates to the technical field of anti-depression products, and in particular to application of rubber seed oil in the preparation of anti-depression products. Background Art

[0002] Depression is a widespread chronic mental illness that causes patients to feel frustrated, helpless, lose interest and pleasure, and affect their thoughts, emotions, and physical health. Symptoms of depression include low mood, insomnia or excessive sleep, changes in appetite, fatigue, inattention, inferiority complex, self-blame, negative thinking, etc. The causes of depression may be multifaceted, including genetic factors, biochemical factors, environmental factors, and other factors. With the increase in work pressure and the tension in interpersonal relationships, the number of patients with depression has increased year by year, seriously affecting people's normal work and life.

[0003] Drug therapy is one of the important means of treating depression. Antidepressants, such as fluoxetine, paroxetine, sertraline, etc., usually relieve symptoms by regulating the level of neurotransmitters in the brain. However, these drugs often bring side effects, such as gastrointestinal symptoms such as nausea, vomiting, diarrhea, indigestion, and cardiovascular effects and sexual dysfunction. In addition, antidepressants are also relatively expensive. Therefore, finding alternative antidepressant strategies with fewer side effects and lower prices is an urgent problem that technicians in this field need to solve.

[0004] Natural plant extracts are one of the important research and development directions of antidepressants. Rubber seeds are a byproduct of natural rubber planting. The oil content of the seed kernel is 40%~50%. After pressing, rubber seed meal and rubber seed oil (RSO) are produced. RSO is abundant and cheap, and is a potential edible renewable oil. The fatty acids in RSO are mainly linoleic acid (C18:2), linolenic acid (C18:3), oleic acid (C18:1), stearic acid (C18:0) and palmitic acid (C16:0), of which polyunsaturated fatty acids account for 65.77%. Compared with commonly used animal-derived feed oils, its unsaturated fatty acid content is higher and the price is lower. The content of linolenic acid is as high as 28.72%, which is 3~4 times that of soybean oil. However, there are few reports on the research of rubber seed oil in the field of antidepressants. Summary of the invention

[0005] In view of the technical problem that there are few reports on the research of rubber seed oil in the field of anti-depression, the present invention provides an application of rubber seed oil in the preparation of an anti-depression product.

[0006] The technical solution of the present invention is as follows: The application of rubber seed oil in the preparation of antidepressant products, wherein the antidepressant products are preferably foods, health products, feeds, feed additives and / or medicines.

[0007] Further, the antidepressant product contains rubber seed oil as the only active ingredient.

[0008] Furthermore, antidepressant products also include excipients, such as carriers, diluents, preservatives, solubilizers, stabilizers, disintegrants, binders, lubricants, wetting agents, emulsifiers, sweeteners, colorants, flavoring agents, salts, buffers, coating agents, antioxidants, etc.

[0009] Furthermore, the antidepressant product is in an oral form, such as tablets, powders, granules, capsules, sustained-release preparations, pills, emulsions, oral solutions, suspensions or aerosols, etc., and the dosage form is preferably an emulsion.

[0010] The beneficial effects of the present invention are: The present invention creatively proposes the therapeutic application of rubber seed oil in alleviating depression, and confirms through mouse experiments that rubber seed oil can effectively alleviate the symptoms of depression and improve the quality of life of patients, providing a safer and more effective treatment plan for patients with depression. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0012] Figure 1 This is a statistical graph of the sucrose preference index results of each group of mice in the sucrose preference experiment.

[0013] Figure 2 This is a statistical chart showing the number of times each group of mice crossed the grid in the open field test.

[0014] Figure 3 This is a statistical chart of the number of upright standing times of mice in each group in the open field test.

[0015] Figure 4 This is a statistical chart of the immobility time of each group of mice in the forced swimming test.

[0016] Figure 5 This is a statistical chart of the immobility time of each group of mice in the tail suspension test. DETAILED DESCRIPTION

[0017] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0018] In each embodiment of the specific implementation manner of the present invention, rubber seed oil is obtained by the following method: The rubber seeds are dried in the sun, shelled and cold pressed to obtain crude oil, and the crude oil is decolorized, deodorized, deacidified and detoxified to obtain rubber seed oil; Commercially available rubber seed oil products may also be used to prepare antidepressant products.

[0019] In each test example of the specific implementation of the present invention, the test data were analyzed using SPSS 25.0 software T-test, # represents the difference comparison between the positive control group and the negative control group, where "#" represents P <0.05, “##” indicates P <0.01, “###” means P <0.001. * indicates the difference between the low, medium and high doses of rubber seed oil treatment groups and the positive control group, "*" indicates P <0.05, “**” indicates P <0.01, "***" indicates P <0.001.

[0020] Example 1 An antidepressant product, comprising rubber seed oil, is prepared as follows: Disperse 0.09 g CMC-Na powder (sodium carboxymethyl cellulose, 300-800 viscosity) in cold distilled water, heat and stir at 80°C to dissolve, and dilute to 300 mL to obtain 0.03% CMC-Na solution; mix 3.45 g rubber seed oil and 0.03% CMC-Na solution evenly and dilute to 20 mL.

[0021] Example 2 An antidepressant product, comprising rubber seed oil, is prepared as follows: Disperse 0.09 g CMC-Na powder (300-800 viscosity) in cold distilled water, heat and stir at 80°C to dissolve, and dilute to 300 mL to obtain 0.03% CMC-Na solution; mix 6.9 g rubber seed oil and 0.03% CMC-Na solution evenly and dilute to 20 mL.

[0022] Example 3 An antidepressant product, comprising rubber seed oil, is prepared as follows: Disperse 0.09 g CMC-Na powder (300-800 viscosity) in cold distilled water, heat and stir at 80°C to dissolve, and dilute to 300 mL to obtain 0.03% CMC-Na solution; mix 13.8 g rubber seed oil and 0.03% CMC-Na solution evenly and dilute to 20 mL.

[0023] Application Example 1 Experimental animals: 120 7-week-old C57BL / 6J male mice were selected after 1 week of adaptation and randomly divided into 6 groups, including negative control group (C-CMCNa), positive control group (M-CMCNa), fluoxetine treatment group (fluoxetine, 20 mg / kg), low-dose rubber seed oil treatment group (LRSO, 1.5 g / kg), medium-dose rubber seed oil treatment group (MRSO, 3 g / kg), and high-dose rubber seed oil treatment group (HRSO, 6 g / kg). There were 12 mice in each group, and 3 mice were kept in each cage. The feeding conditions were: 12h dark / light cycle, room temperature 23±1℃, humidity 50%±5%, free drinking water and food. The negative control group was placed in a separate room.

[0024] Drug treatment was carried out from the first day of the stress experiment until the mice were killed. The negative control group and the positive control group were gavaged with 200 μL of 0.03% CMC-Na solution (prepared in the same way as in Examples 1-3) per kg body weight per day, and the other treatment groups were gavaged with 200 μL of the corresponding reagents, wherein the reagent used in the fluoxetine treatment group was a physiological saline solution of 2.3 mg / mL fluoxetine, which was prepared and used immediately; the reagent used in the low-dose rubber seed oil treatment group was the antidepressant product prepared in Example 1; the reagent used in the medium-dose rubber seed oil treatment group was the antidepressant product prepared in Example 2; and the reagent used in the high-dose rubber seed oil treatment group was the antidepressant product prepared in Example 3.

[0025] The negative control group was not treated with chronic unpredictable mild stress (CUMS), and the other groups were treated with CUMS 4 hours after gavage to prevent mice from vomiting. The gavage time and CUMS time were fixed every day.

[0026] Mice treated with CUMS were randomly assigned to receive one of the stressors every day, with no stressor applied for two consecutive days, and the stress procedure lasted for 5 weeks. The stressors were selected from: ① No food and water for 24 h; ② Cage tilted at 30° for 24 h; ③ Light on all night; ④ Tail clamped for 1 min (1 cm from the tip of the tail); ⑤ Crowded together for 24 h; ⑥ Wet cage for 24 h: 200 mL of water added to 100 g of sawdust bedding; ⑦ Body restraint for 2 h.

[0027] Behavioral tests of depressed mice: Sucrosepreference test (SPT) was performed on the 36th day after the stress experiment, open field test (OFT) was performed on the 38th day, forced swin test (FST) was performed on the 40th day, and tail suspension test (TST) was performed on the 42nd day. Only one test was performed per day, with an interval of about 24 hours between tests.

[0028] 1. Sucrose preference experiment 72 h before the test, mice were trained to adapt to 1% sucrose solution: 2 bottles of 1% sucrose solution were placed in each cage, and 1 bottle of 1% sucrose was replaced with water after 24 h for 24 h. After the adaptation, mice were fasted but not watered for 24 h, and then the sucrose preference experiment was performed. Each cage of mice was free to eat two bottles of 100 mL of 1% sucrose solution and 100 mL of tap water. After 12 h, the sugar water consumption was recorded, and the sugar water preference (%) was calculated = sugar water consumption / (sugar water consumption + water consumption).

[0029] The results are as follows Figure 1 As shown in the figure, compared with the negative control group, the sucrose preference index of the positive control group was significantly reduced, indicating that the mice in the positive control group had stronger depressive symptoms, while the indicators of the mice in the fluoxetine treatment group using the antidepressant fluoxetine improved. After intervention with different concentrations of rubber seed oil, the behavioral indicators of mice improved. Among them, the effect of the high-dose rubber seed oil treatment group was the most obvious, and there was no significant difference with the fluoxetine treatment group, indicating that rubber seed oil can be used to develop products for the treatment of depression.

[0030] 2. Open Field Experiment To evaluate the possible effects of rubber seed oil on spontaneous locomotor activity, the open field test was performed on each group of mice. The test was performed under bright and quiet ambient room light. The open field apparatus consisted of a four-sided black wooden box (96×96×50 cm), with the floor divided into 16 equal squares by white lines, and the central 4 squares were defined as the central area. Mice were placed individually in the central area and allowed to freely explore the unfamiliar arena for 5 min. During this period, the total number of squares crossed by the mice and the number of rearing times (hind legs upright) were manually recorded. The open field apparatus was cleaned and dried after each mouse entered the room.

[0031] The results are as follows Figure 2 and Figure 3As shown in the figure, compared with the negative control group, the number of square crossings and upright times in the open field in the positive control group was significantly reduced, indicating that the mice in the positive control group had stronger depressive symptoms, while the indicators of the mice in the fluoxetine treatment group using the antidepressant fluoxetine improved. After intervention with different concentrations of rubber seed oil, the behavioral indicators of mice improved, among which the high-dose rubber seed oil treatment group had the most obvious effect, and there was no significant difference between the high-dose rubber seed oil treatment group and the fluoxetine treatment group, indicating that rubber seed oil can be used to develop products for the treatment of depression.

[0032] 3. Forced swimming test The mice were placed individually in a transparent glass cylinder 25 cm high and 10 cm in diameter, which contained 10 cm of fresh water, and subjected to a forced swimming test for 6 minutes at 23±2°C. After the first two minutes of intense struggling, the immobility time was recorded for the next 4 minutes, and the mice were considered immobile when they floated in the water without struggling and only made necessary movements.

[0033] The results are as follows Figure 4 As shown in the figure, compared with the negative control group, the immobility time of the positive control group in the forced swimming test was significantly increased, indicating that the mice in the positive control group had stronger depressive symptoms, while the indicators of the mice in the fluoxetine treatment group using the antidepressant fluoxetine improved. After intervention with different concentrations of rubber seed oil, the behavioral indicators of mice improved. Among them, the effect of the high-dose rubber seed oil treatment group was the most obvious, better than the fluoxetine treatment group, indicating that rubber seed oil can be used to develop products for the treatment of depression.

[0034] 4. Tail suspension test Place tape approximately 1 cm from the tip of the mouse's tail to suspend the acoustically and visually isolated mouse 50 cm above the ground. During the 6-min test period, record the time the mouse remains motionless.

[0035] The results are as follows Figure 5 As shown in the figure, compared with the negative control group, the immobility time of the positive control group in the tail suspension test was significantly increased, indicating that the mice in the positive control group had stronger depressive symptoms, while the indicators of the mice in the fluoxetine treatment group using the antidepressant fluoxetine improved. After intervention with different concentrations of rubber seed oil, the behavioral indicators of mice improved, among which the high-dose rubber seed oil treatment group had the most obvious effect, indicating that rubber seed oil can be used to develop products for the treatment of depression.

[0036] Although the present invention has been described in detail with reference to the accompanying drawings and in combination with the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person of ordinary skill in the art may easily think of changes or substitutions within the technical scope disclosed by the present invention, and these shall be within the scope of protection of the present invention.

Claims

1. Application of rubber seed oil in the preparation of antidepressant products.

2. The use according to claim 1, characterized in that The antidepressant product is a food, a health product, a feed, a feed additive and / or a medicine.

3. The use according to claim 1, characterized in that Antidepressant product with rubber seed oil as the only active ingredient.

4. The use according to claim 3, characterized in that Antidepressant products also include excipients.

5. The use according to claim 1, characterized in that Antidepressant products come in oral form.

6. The use according to claim 5, characterized in that The dosage form of the antidepressant product is a lotion.

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