Prestressed blood vessel chip

Abstract

The invention discloses a prestress blood vessel chip, and relates to the technical field of medical blood vessels, the prestress blood vessel chip comprises a body, the surface of the body is provided with a first chamber and a third chamber, the first chamber is communicated with the third chamber through a first channel, the body is provided with at least two fourth chambers, and the fourth chambers are arranged on one side of the first chamber; a communicating channel is formed between the fourth cavities, and at least two first channels communicating with the first cavities are formed in the communicating channel. The blood vessel chip can achieve the state that the mechanical property form is similar to the in-vivo blood vessel mechanical property; the problem that the mechanical property state of a blood vessel channel in a chip in the prior art is greatly different from the real blood vessel state of a human body is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical blood vessels, and in particular to a prestressed blood vessel chip. Background Art

[0002] Vascular chip is a typical microfluidic chip. In addition to the characteristics of miniaturization, integration, and low power consumption, vascular chip technology can also accurately control multiple system parameters. Such as chemical concentration gradients, fluid shear force, as well as the construction of cell pattern culture, tissue-tissue interface interaction, etc., to simulate the complex structure, microenvironment and physiological function of human blood vessels. Currently, the most important treatment for cardiovascular disease is interventional therapy, among which the implantation of vascular stents is the most commonly used means of interventional therapy. The development of new vascular stents that are more suitable for the complex internal environment of the human body is of great significance to alleviating the increasingly severe incidence of cardiovascular diseases.

[0003] At present, relevant evaluation and research experiments are usually limited to animal model tests. However, there are many limiting factors in animal experiments, such as the control of animal physiological state and interventional surgical conditions, the cost of feeding experimental animals and animal model construction, as well as the disadvantages of long preparation period and complicated preparation procedures. PDMS (polydimethylsiloxane) is one of the most common microfluidic chip materials at present. PDMS has excellent softness, transparency and biocompatibility, and is easy to process and prepare microfluidic structures. Therefore, it is widely used in microfluidic chip research and applications in biomedicine, chemical analysis and other fields. PDMS microfluidic chips can realize the precise control and operation of tiny fluids, and have the advantages of high throughput, high sensitivity and high integration. Therefore, they have been widely used in biological analysis, cell culture, drug screening and other fields. At present, vascular chip technology is developing rapidly, and various methods are emerging, including vascularized organoid chips under fluid stimulation, 3D printed pipeline structures assisted by winding rods, etc.

[0004] To solve the above problems, the prior art provides more solutions. For example, the prior art US18678112 discloses a microfluidic device, which includes a microfluidic chip for producing micro organic spheres (MOS). The first channel is defined in the surface of the microfluidic chip and includes: a droplet generating part, the droplet generating part includes an inlet part, a junction between the inlet part and the emulsified fluid channel, and a chamber downstream of the junction. The cross-sectional area of ​​the chamber is larger than the cross-sectional area of ​​the inlet part. The first channel includes a polymerization part downstream of the droplet generating part, and the polymerization part has a serpentine structure. The device includes a barrel for MOS demulsification, and the barrel includes: a collection container; a substrate disposed on the collection container, and a membrane disposed between the collection container and the surface of the substrate. The second channel is defined in the surface of the substrate facing the collection container and is connected to the output fluid of the polymerization part of the first channel, but the device still has room for improvement in controlling the inflow of the medium. Summary of the invention

[0005] The purpose of the present invention is to provide a prestressed vascular chip, which can achieve a mechanical performance morphology similar to the mechanical properties of blood vessels in the body, so as to solve the problem that the mechanical performance state of the vascular channel inside the chip of the prior art is quite different from the actual vascular state of the human body.

[0006] In order to solve the above technical problems, the present invention specifically provides the following technical solutions: a prestressed blood vessel chip, comprising a body, a first chamber and a third chamber are opened on the surface of the body, the first chamber is connected to the third chamber through a first channel, the body is provided with at least two fourth chambers, the fourth chamber is provided on one side of the first chamber, and there is a connecting channel between the fourth chambers, and at least two first channels connected to the first chamber are provided on the connecting channel. The present invention designs the first chamber for cell culture or testing, and the first channel is provided for simulating blood vessels. By setting at least two fourth chambers, when the culture fluid or medium is input, the output pressure formed by the medium after passing through the fourth chamber to the first chamber and the third chamber is different, and the flow rate of the medium entering each fourth chamber through the connecting channel is different, and the pressure and flow rate of the medium flowing out of these fourth chambers are different, so that the first channel and the subsequent flow path are subjected to different pressures, and the simulated blood vessels are subjected to different reactions under different media and different flow rates, so that the medium input is close to the recognition of the real blood vessel state difference.

[0007] At the same time, the above design can provide a variety of liquid inlet solutions, and it is also possible to choose to retain only a single fourth chamber and a single connecting channel. The specific data can be freely selected according to the experimental purpose, and the experimental application range is relatively wide.

[0008] According to one embodiment of the present invention, the main body has an extension plate on the side, the extension plate is connected to the first tube, and the communication channel between the first tube and the fourth chamber is connected. The first tube is a capillary tube for supplying medium to the fourth chamber, and the extension plate is provided to stabilize the input at the interface between the first tube and the fourth chamber, thereby solving the problem that the first tube may be bent at the fourth chamber, resulting in inconsistency between the inflow flow rate, flow rate, pressure and the pump input flow rate, flow rate, pressure, etc.

[0009] According to one embodiment of the present invention, the fourth chamber is a circular chamber, and protrusions with intervals are arranged in an array around the fourth chamber, and the side of the protrusion has an arc surface structure. The fourth chamber is set as a circular structure and is equipped with protrusions arranged in an array around it, so that when the medium enters the circular fourth chamber, it can be guided by the circular structure and the arc surface structure of the protrusion to form a fluid with a regular flow rate and flow direction. The medium can form a vortex inside the fourth chamber through multiple protrusions and be discharged from the outlet direction of the fourth chamber. In this process, the mixing effect of the medium is better to avoid the blockage of the simulated blood vessel flow path caused by the appearance of lumps in the medium. Based on the different flow rates inside the fourth chamber, different fourth chambers can form different output pressures to the first chamber and the third chamber. More importantly, the protrusion structure solves the problem that there may be turbulence in the medium input or the irregularity of the short-term fluid cannot simulate the real blood vessel state of the human body.

[0010] According to one embodiment of the present invention, there is a second chamber between the first chamber and the third chamber, and the second chamber is connected to the first chamber and the third chamber respectively through the first channel. The second chamber is set to simulate multiple organs inside the human body, the first chamber and the second chamber form multiple culture spaces, and the third chamber forms a medium discharge temporary storage space. Compared with the single culture simulation space in the prior art, the present invention provides more test options and higher accuracy.

[0011] According to one embodiment of the present invention, a collecting piece connected to the third chamber is provided below the main body. The collecting piece is a tubular structure and is used to collect waste liquid inside the third chamber. By arranging the collecting piece below the third chamber, gravity is utilized to improve the waste liquid discharge efficiency inside the third chamber. The optional use of a pump body can control the waste liquid discharge rate inside the third chamber.

[0012] According to one embodiment of the present invention, a support body is provided below the body, a support plate connected to the support body is provided on one side of the body, the contact surface between the support body and the body is a flat surface, and the end surface away from the body is an inclined surface. The support body and the support plate are used to ensure the flatness of the body to prevent the body from bending, and further the inclined surface of the support body can be installed with a hydraulic rod to adjust the inclination angle of the support body to control the inclination angle of the body compared to the horizontal plane, providing different test parameter conditions.

[0013] According to one embodiment of the present invention, there are at least two bodies, and adjacent bodies are spaced apart and arranged in an array, thereby providing multiple test options and being able to expand the processing capacity.

[0014] A method for preparing a prestressed vascular chip is provided, and the steps of the preparation method are as follows: Mask making: Build a model of the positive film part of the target chip. Due to the difference in the upper and lower double-layer structures, two positive molds with different structural designs need to be made to obtain a chip with different upper and lower layer structures. Prepare the positive film part on the silicon wafer through a photolithography machine and prepare the PDMS mixture: mix the PDMS base liquid and the cross-linking agent in proportion to obtain a uniform mixture; Degassing: Place the mixed PDMS mixture in a vacuum oven for degassing; leave the mixture to stand for a period of time under vacuum conditions to allow the bubbles in it to be completely discharged. PDMS coating: Evenly coat the degassing PDMS mixture on a silicon wafer or glass sheet. You can use a scraper or roller coating method to ensure that the coating is uniform and of consistent thickness.

[0015] PDMS coating: evenly coat the de-bubbled PDMS mixture on a silicon wafer or glass sheet; Prestressed fiber material laying: The fiber material is stretched, and the pre-stretched fiber material is laid on the upper layer of PDMS, and the stretched state is fixed; various fiber materials are used to manufacture vascular prostheses, and the fiber material is stretched to a certain extent, and the pre-stretched polymer fiber material is laid on the upper layer of PDMS, and the stretched state is fixed.

[0016] Baking and cross-linking: Put the silicon wafer or glass wafer coated with PDMS into an oven for baking and cross-linking; Cutting the chip: After the PDMS is completely cross-linked, use a knife or laser cutting method to cut the PDMS chip into the desired shape and size.

[0017] Cleaning treatment: Put the two prepared PDMS chips into a plasma cleaning machine, and bond the two PDMS chips to other materials to form a complete microfluidic vascular chip with a prestressed state.

[0018] The PDMS base liquid and the cross-linking agent are mixed in a ratio of 5-10:1. The preferred method is: Prepare the PDMS mixture: Mix the PDMS base liquid and the cross-linking agent in a ratio of 10:1, and mix thoroughly until a uniform mixture is obtained.

[0019] The crosslinking is baked at a temperature of about 80 degrees Celsius for 0.5-4 hours, preferably 2 hours.

[0020] The present invention can simulate the external soft tissue constraint state when the vascular channel is deformed by adding a material similar to human soft tissue to the periphery of the vascular channel. This solves the problem that the existing microfluidic chip technology cannot present the state of vascular deformation. The present invention can change the configuration of the vascular channel by adding pre-stretched linear polymer materials to the peripheral area of ​​the blood vessel by stretching the wire.

[0021] Compared with the prior art, the present invention has the following beneficial effects: the present invention designs a first chamber for cell culture or testing, and provides a first channel for simulating blood vessels. By setting at least two fourth chambers, when the culture fluid or medium is input, the output pressure formed by the medium to the first chamber and the third chamber after passing through the fourth chamber is different, and the flow rate of the medium entering each fourth chamber through the connecting channel is different, and the pressure and flow rate of the medium flowing out of these fourth chambers are different. In this way, the first channel and the subsequent flow path are subjected to different pressures, simulating the different reactions of blood vessels to different media and different flow rates, so that the medium input is close to the recognition of the real blood vessel state difference.

[0022] The vascular chip constructed by the present invention can achieve a state in which the mechanical performance morphology is similar to the mechanical properties of blood vessels in the body, so as to solve the problem that the mechanical performance state of the vascular channel inside the chip of the prior art is greatly different from the actual blood vessel state of the human body. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0024] Figure 1 This is a schematic diagram of a combined use state of a prestressed vascular chip of the present invention; Figure 2 This is a schematic diagram of a prestressed vascular chip of the present invention; Figure 3 It is a schematic diagram of the fourth chamber on the body of the present invention; Figure 4 A top view of the fourth chamber on the body of the present invention; Figure 5 It is a schematic diagram of the connecting piece scheme of the present invention; Figure 6 is a cross-sectional view of a second connection base of the present invention; Figure 7 It is a schematic diagram of the limiting member solution of the present invention; Figure 8 It is a cross-sectional view of the limiting member of the present invention.

[0025] Description of reference numerals: 10. Main body; 11. Collecting piece; 12. Third chamber; 13. Second chamber; 14. First chamber; 15. First channel; 16. Support body; 17. Extension plate; 18. Fourth chamber; 19. Protrusion; 20. Connecting piece; 21. First connecting base; 221. First hole body; 22. Second connecting base; 23. Second hole body; 24. Annular groove; 25. Third hole body; 26. Fourth hole body; 30. First tube body; 40. Limiting piece; 41. Fifth hole body; 42. Groove body; 43. Limiting inner tube; 44. Filling ring. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The following first describes the concepts involved in the present application in conjunction with the accompanying drawings. It should be noted that the following description of each concept is only to make the content of the present application easier to understand, and does not limit the scope of protection of the present application; at the same time, the embodiments and features in the embodiments of the present application can be combined with each other in the absence of conflict. The present application will be described in detail with reference to the accompanying drawings and in conjunction with the embodiments.

[0028] Embodiment 1 like Figure 1-8 As shown, a prestressed vascular chip comprises a main body 10, a first chamber 14 and a third chamber 12 are provided on the surface of the main body 10, the first chamber 14 is connected to the third chamber 12 via a first channel 15, the main body 10 is provided with at least two fourth chambers 18, the fourth chamber 18 is provided on one side of the first chamber 14, and a connecting channel is provided between the fourth chambers 18, and at least two first channels 15 connected to the first chamber 14 are provided on the connecting channel. The present invention designs a first chamber 14 for cell culture or testing, and a first channel 15 for simulating blood vessels. At least two fourth chambers 18 are provided to form a culture fluid or medium input, so that the output pressure of the medium to the first chamber 14 and the third chamber 12 after passing through the fourth chamber 18 is different. The flow rate of the medium entering each fourth chamber 18 through the connecting channel is different, and the pressure and flow rate of the medium flowing out of these fourth chambers 18 are different. In this way, the first channel 15 and the subsequent flow path are subjected to different pressures, simulating the different reactions of blood vessels to different media and different flow rates, so that the medium input is close to the recognition of the actual difference in blood vessel status.

[0029] At the same time, the above design can provide a variety of liquid inlet solutions, and it is also possible to choose to only retain a single fourth chamber 18 and a single connecting channel. The specific data can be freely selected according to the experimental purpose, and the experimental application range is relatively wide.

[0030] The body 10 has an extension plate 17 on the side, and the first tube 30 is connected to the extension plate 17, and the communication channel between the first tube 30 and the fourth chamber 18 is connected. The first tube 30 is a capillary tube for supplying the medium to the fourth chamber 18, and the extension plate 17 is provided to stabilize the input at the interface between the first tube 30 and the fourth chamber 18, so as to solve the situation that the first tube 30 may be bent at the fourth chamber 18, resulting in the inflow flow rate, flow rate, pressure and the pump input flow rate, flow rate, pressure and the like being inconsistent.

[0031] The fourth chamber 18 is a circular chamber, and the protrusions 19 with intervals are arranged in an array around the fourth chamber 18, and the side of the protrusion 19 has an arc surface structure. The fourth chamber 18 is set as a circular structure and equipped with protrusions 19 arranged in an array around it, so that when the medium enters the circular fourth chamber 18, it can be guided by the circular structure and the arc surface structure of the protrusion 19 to form a fluid with a regular flow rate and flow direction. The medium can form a vortex inside the fourth chamber 18 through multiple protrusions 19 and be discharged from the outlet direction of the fourth chamber 18. In this process, the mixing effect of the medium is better to avoid the blockage of the simulated blood vessel flow path caused by the appearance of lumps in the medium. Based on the different flow rates inside the fourth chamber 18, different fourth chambers 18 can form different output pressures to the first chamber 14 and the third chamber 12. More importantly, the protrusion 19 structure solves the problem that there may be turbulence in the medium input or the irregularity of the short-term fluid cannot simulate the real blood vessel state of the human body.

[0032] There is a second chamber 13 between the first chamber 14 and the third chamber 12, and the second chamber 13 is connected to the first chamber 14 and the third chamber 12 through the first channel 15. The second chamber 13 is used to simulate multiple organs in the human body, the first chamber 14 and the second chamber 13 form multiple culture spaces, and the third chamber 12 forms a temporary storage space for medium discharge. Compared with the single culture simulation space in the prior art, the present invention provides more test options and higher accuracy.

[0033] A collecting piece 11 connected to the third chamber 12 is provided below the main body 10. The collecting piece 11 is a tubular structure and is used to collect waste liquid inside the third chamber 12. By arranging the collecting piece 11 below the third chamber 12, gravity is utilized to improve the waste liquid discharge efficiency inside the third chamber 12. The optional use of a pump body can control the waste liquid discharge rate inside the third chamber 12.

[0034] A support body 16 is provided below the body 10, and a support plate connected to the support body 16 is provided on one side of the body 10. The contact surface between the support body 16 and the body 10 is a flat surface, and the end surface away from the body 10 is an inclined surface. The support body 16 and the support plate are used to ensure the flatness of the body 10 to prevent the body 10 from bending. Furthermore, the inclined surface of the support body 16 can be installed with a hydraulic rod to adjust the inclination angle of the support body 16 to control the inclination angle of the body 10 compared to the horizontal plane, providing different test parameter conditions.

[0035] There are at least two main bodies 10 , and adjacent main bodies 10 are spaced apart and arranged in an array, providing multiple test options while being able to expand the processing capacity.

[0036] A method for preparing a prestressed vascular chip is provided, and the steps of the preparation method are as follows: Mask making: Build a model of the positive film part of the target chip. Due to the difference in the upper and lower double-layer structures, two positive molds with different structural designs need to be made to obtain a chip with different upper and lower layer structures. Prepare the positive film part on the silicon wafer through a photolithography machine and prepare the PDMS mixture: mix the PDMS base liquid and the cross-linking agent in proportion to obtain a uniform mixture; Degassing: Place the mixed PDMS mixture in a vacuum oven for degassing; leave the mixture to stand for a period of time under vacuum conditions to allow the bubbles in it to be completely discharged. PDMS coating: Evenly coat the degassing PDMS mixture on a silicon wafer or glass sheet. You can use a scraper or roller coating method to ensure that the coating is uniform and of consistent thickness.

[0037] PDMS coating: evenly coat the de-bubbled PDMS mixture on a silicon wafer or glass sheet; Laying of prestressed fiber materials: The fiber materials are stretched and laid on the upper layer of PDMS, and the stretched state is fixed; various textile materials and fiber materials are used to manufacture vascular prostheses, including nylon polyurethane or spandex, polyvinyl acetal (Ivalon®) and acrylic (Orlon®), polyethylene terephthalate (PET) or polyester (Dacron®) and polytetrafluoroethylene or PTFE (Tefl on®) braided or knitted textile tubes or porous expanded membrane structures. The fiber materials are stretched to a certain extent, and the pre-stretched polymer fiber materials are laid on the upper layer of PDMS, and the stretched state is fixed.

[0038] Baking and cross-linking: Put the silicon wafer or glass wafer coated with PDMS into an oven for baking and cross-linking; Cutting the chip: After the PDMS is completely cross-linked, use a knife or laser cutting method to cut the PDMS chip into the desired shape and size.

[0039] Cleaning treatment: Put the two prepared PDMS chips into a plasma cleaning machine, and bond the two PDMS chips to other materials to form a complete microfluidic vascular chip with a prestressed state.

[0040] The PDMS base liquid and the cross-linking agent are mixed in a ratio of 5-10:1. The preferred method is: Prepare the PDMS mixture: Mix the PDMS base liquid and the cross-linking agent in a ratio of 10:1, and mix thoroughly until a uniform mixture is obtained.

[0041] The crosslinking is baked at a temperature of about 80 degrees Celsius for 0.5-4 hours.

[0042] Embodiment 2: See attached Figure 2 , Attachment Figure 5 , Attachment Figure 6 As shown, this embodiment provides a further solution based on the substantial example 1, a connector 20 is connected to the top of the fourth chamber 18 through a sealing ring, the connector 20 includes a second connecting base 22 connected to the inner wall of the upper end of the fourth chamber 18, and a first connecting base 21 is coaxially assembled on the upper part of the second connecting base 22, and both the first connecting base 21 and the second connecting base 22 are cylindrical structures.

[0043] A sealing ring structure is provided at the connection between the second connecting base 22 and the fourth chamber 18, a first hole 221 is provided in the middle of the second connecting base 22 and does not pass through the bottom of the second connecting base 22, a fourth hole 26 connected to the fourth chamber 18 is provided at the bottom of the second connecting base 22, the first connecting base 21 has a column plugged into the first hole 221, and another first hole 221 connected to the first hole 221 on the second connecting base 22 is provided on the plugged column.

[0044] The second connection base 22 is surrounded by a second hole 23 , which is arranged around the center of the first hole 221 on the second connection base 22 . The second connection base 22 is provided with a through hole for connecting the second hole 23 with the first hole 221 .

[0045] The bottom surface of the second connection base 22 at the bottom of the second hole 23 is uniformly distributed with micropores.

[0046] An annular groove 24 is arranged around the outside of the second connecting base 22. When it is necessary to supplement the medium or input other fluids, the medium can be directly input into the fourth chamber 18 by inserting the pipeline into the first hole body 221. The pipe body can also be matched with the sealing ring to cover the outside of the connecting piece 20, so that the sealing ring is filled in the annular groove 24 to prevent leakage of the pipe body. The medium can also be input into the first hole body 221, and then discharged from the fourth hole 26 and the micropores at the bottom of the second hole 23 to the fourth chamber 18 below. This design can make the input medium enter the fourth chamber 18 in a diverted manner, solving the problem of turbulence caused by the convergence of the medium sent into the chamber and the original rectified fluid of the fourth chamber 18. In addition, this scheme can weaken the downward impact force of the supplementary input medium and the problem of excessive pressure difference at the interface between the connecting piece 20 and the fourth chamber 18. Specifically, the pressure entering the fourth chamber 18 is reduced by diverting the medium from the fourth hole 26 and the third hole body 25, thereby solving the pressure difference problem at the contact port.

[0047] Embodiment 3: See attached Figure 1 , Attachment Figure 7 , Attachment Figure 8 As shown, this embodiment provides a further solution based on the substantial example 1. The main body 10 of the present invention can be multiple. In this case, the number of the first pipe bodies 30 increases accordingly. There may be problems such as the first pipe bodies 30 being staggered or the pipelines being entangled with other equipment pipelines. For this reason, the attached Figure 7 A limiting member 40 is provided to constrain multiple first tube bodies 30. The limiting member 40 includes a main body of a cylindrical sleeve structure, which is hollow inside and is provided with limiting inner tubes 43 at intervals. The outer wall of the limiting inner tube 43 is connected to the inner wall of the outer cylindrical sleeve structure main body through a filling ring 44. On this basis, a coaxial fifth hole 41 is provided through the middle of the cylindrical sleeve structure main body and the limiting inner tube 43, and a groove body 42 is provided on one side of the cylindrical sleeve structure main body and the limiting inner tube 43. Through the above-mentioned design scheme, multiple first tube bodies 30 can be placed in the fifth hole body 41, so that the layout of multiple first tube bodies 30 is constrained. The increase in the number of tube bodies may cause the diameter of the tube body to be compressed. The groove body 42 provided in the present invention can allow the cylindrical sleeve structure main body and the limiting inner tube 43 to have a certain deformation space after grooving. In this way, after the number of first tube bodies 30 increases, the deformation of the cylindrical sleeve structure main body and the limiting inner tube 43 provides appropriate space to avoid the diameter of the first tube body 30 being compressed.

[0048] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any form. Any technical personnel in this field may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0049] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and its core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the concept and technical solution of the invention to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A prestressed vascular chip, comprising a body (10), wherein a first chamber (14) and a third chamber (12) are formed on the surface of the body (10), wherein the first chamber (14) is connected to the third chamber (12) through a first channel (15), and wherein: The body (10) is provided with at least two fourth chambers (18), the fourth chambers (18) being provided on one side of the first chamber (14), and a connecting passage is provided between the fourth chambers (18), and at least two first passages (15) communicating with the first chamber (14) are provided on the connecting passage.

2. The prestressed vascular chip according to claim 1, characterized in that: An extension plate (17) is provided on the side of the main body (10), a first tube body (30) is connected to the extension plate (17), and a communication channel between the first tube body (30) and the fourth chamber (18) is connected.

3. The prestressed vascular chip according to claim 1, characterized in that: The fourth chamber (18) is a circular chamber, and projections (19) are arranged in an array at intervals around the fourth chamber (18), and the side surfaces of the projections (19) have an arc-shaped surface structure.

4. The prestressed vascular chip according to claim 1, characterized in that: A second chamber (13) is provided between the first chamber (14) and the third chamber (12), and the second chamber (13) is communicated with the first chamber (14) and the third chamber (12) respectively through a first channel (15).

5. The prestressed vascular chip according to claim 1, characterized in that: A collecting piece (11) in communication with the third chamber (12) is provided below the main body (10); the collecting piece (11) is a tubular structure.

6. The prestressed vascular chip according to claim 1, characterized in that: A support body (16) is provided below the body (10); a support plate connected to the support body (16) is provided on one side of the body (10); a contact surface between the support body (16) and the body (10) is a flat surface, and an end surface facing away from the body (10) is an inclined surface.

7. The prestressed vascular chip according to claim 1, characterized in that: There are at least two bodies (10), and adjacent bodies (10) are spaced apart and arranged in an array.

8. A method for preparing a prestressed vascular chip according to any one of claims 1 to 7, characterized in that: The preparation method steps are as follows: Mask making: construct a model of the positive film part of the target chip, prepare the positive film part on the silicon wafer by using a photolithography machine, prepare the PDMS mixture: mix the PDMS base liquid and the cross-linking agent in proportion to obtain a uniform mixture; Degassing treatment: Place the mixed PDMS mixture in a vacuum oven for degassing treatment; PDMS coating: evenly coat the de-bubbled PDMS mixture on a silicon wafer or glass sheet; Laying of prestressed fiber materials: stretching the fiber materials, laying the pre-stretched fiber materials on the upper layer of PDMS, and fixing the stretched state; Baking and cross-linking: Put the silicon wafer or glass wafer coated with PDMS into an oven for baking and cross-linking; Cutting the chip: After the PDMS is completely cross-linked, use a knife or laser cutting method to cut the PDMS chip.

9. The method for preparing a prestressed vascular chip according to claim 8, characterized in that: PDMS base liquid and cross-linking agent are mixed in a ratio of 5-10:

1.

10. The method for preparing a prestressed vascular chip according to claim 8, characterized in that: The crosslinking is baked at a temperature of about 80 degrees Celsius for 0.5-4 hours.

Images