Application of white kidney bean extract or composition thereof in promoting lipolysis

Abstract

The invention discloses an application of a white kidney bean extract or a composition thereof in promoting lipolysis, provides a new application of the white kidney bean extract or the composition thereof, and particularly relates to an application of the white kidney bean extract or the composition thereof in preparing a product for promoting lipolysis. Meanwhile, according to the composition for promoting lipolysis provided by the invention, the white kidney bean extract and the bifidobacterium animalis subsp. Lactis MN-Gup are matched with each other, so that the composition has a synergistic effect on the function of promoting lipolysis.

Description

Technical Field

[0001] The invention relates to a new application field of white kidney bean, and in particular to application of a white kidney bean extract or a composition thereof in promoting fat decomposition. Background Art

[0002] White kidney bean is a common edible bean. White kidney bean is not only rich in nutritional value, but also has high medicinal and health value. It is a traditional food and medicine in my country. White kidney bean extract is a natural amylase inhibitor extracted from white kidney bean. Its active ingredient is mainly α-amylase inhibitor, which is a glycoside hydrolase inhibitor. The currently disclosed white kidney bean extract inhibits the activity of saliva and pancreatic amylase in the intestine, hinders the digestion and absorption of carbohydrates in food, and selectively reduces sugar intake, thereby playing a role in lowering blood sugar, blood lipids, and reducing the conversion of sugar to fat. Summary of the invention

[0003] Therefore, the object of the present invention is to provide a new use of a white kidney bean extract or a composition thereof, and specifically, to provide a use of a white kidney bean extract or a composition thereof in the preparation of a product that promotes fat decomposition.

[0004] Application of white kidney bean extract or a composition thereof in preparing a product promoting fat decomposition.

[0005] The composition includes white kidney bean extract and Bifidobacterium animalis subsp. lactis MN-Gup.

[0006] The mass ratio of the white kidney bean extract to animal Bifidobacterium lactis subsp. MN-Gup is 1:(0.05-20), for example, it can be 1:0.05, 1:0.1, 1:0.5, 1:1, 1:3, 1:5, 1:7, 1:9, 1:10, 1:12, 1:14, 1:16, 1:18, 1:20 or any ratio therein not shown.

[0007] The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 CFU / g or more, for example: 4×10 9 CFU / g, 5×10 9 CFU / g, 6×10 9 CFU / g, 7×10 9 CFU / g, 8×10 9 CFU / g, 9×10 9 CFU / g, 10×10 9 CFU / g, 20×10 9 CFU / g, 30×10 9 CFU / g, 50×10 9CFU / g, 80×10 9 CFU / g, 100×10 9 CFU / g, 150×10 9 CFU / g, 200×10 9 CFU / g, 250×10 9 CFU / g, 300×10 9 CFU / g, 350×10 9 CFU / g, 400×10 9 CFU / g, 450×10 9 CFU / g, 500×10 9 CFU / g, 600×10 9 CFU / g, etc. or any content not shown therein between the viable counts.

[0008] The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 ~500×10 9 CFU / g.

[0009] A composition for promoting fat decomposition comprises white kidney bean extract and Bifidobacterium animalis subspecies lactis MN-Gup.

[0010] The mass ratio of the white kidney bean extract to animal Bifidobacterium lactis subspecies MN-Gup is 1:(0.05-20).

[0011] The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 CFU / g and above.

[0012] The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 ~500×10 9 CFU / g.

[0013] The technical solution of the present invention has the following advantages:

[0014] The present invention provides a new use of a white kidney bean extract or a composition thereof, specifically, the use of the white kidney bean extract or the composition thereof in preparing a product that promotes fat decomposition. At the same time, the composition for promoting fat decomposition provided by the present invention has a synergistic effect in promoting fat decomposition through the cooperation between the white kidney bean extract and the animal Bifidobacterium lactis subspecies MN-Gup. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 This is a diagram showing the fat decomposition results of the white kidney bean extract of the present invention;

[0017] Figure 2 This is a diagram showing the results of fat decomposition of the composition of the white kidney bean extract of the present invention. DETAILED DESCRIPTION

[0018] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0019] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0020] Example 1

[0021] Application of white kidney bean extract in preparing products promoting fat decomposition.

[0022] The white kidney bean extract in the present invention can be extracted by oneself or purchased from a commercial product, for example, the white kidney bean extract purchased from Yunnan Tianbaohua Biological Resources Development Co., Ltd.

[0023] Application effect detection:

[0024] 1. Experimental Animals

[0025] Zebrafish were raised in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / LCaCO3).

[0026] 2. Detection method

[0027] 2.1. Determination of maximum detectable concentration (MTC)

[0028] Melanin allele mutant zebrafish (albino) at 2dpf (days of embryonic development) were randomly selected in a 6-well plate, with 30 zebrafish in each well (experimental group). White kidney bean extract and animal Bifidobacterium lactis subsp. MN-Gup were given respectively, and the concentrations given were shown in Table 1. A normal control group was also set up, with a volume of 3mL per well. After 1 day of treatment at 28℃, each experimental group was given Nile red dye in water, and the treatment was continued at 28℃ until 4dpf, and the MTC of normal zebrafish in the samples was determined.

[0029] 2.2 Evaluation of the effect of white kidney bean extract on promoting lipolysis

[0030] White kidney bean extract was divided into low, medium and high dose groups. The low dose group (BYD-L) of white kidney bean extract was given a sample concentration of 12.5 μg / mL, the medium dose group (BYD-M) was given a sample concentration of 25.0 μg / mL, and the high dose group (BYD-H) was given a sample concentration of 50.0 μg / mL.

[0031] 2dpf melanin allele mutant zebrafish (albino) were randomly selected in 6-well plates, and 30 zebrafish were treated in each well. The water-soluble group was given the corresponding dose of white kidney bean extract in the low, medium and high dose groups, and the normal group was set up as a control; after 1 day of treatment at 28℃, each group was given Nile red dye in water-soluble form, and the treatment was continued at 28℃ until 4dpf. Ten zebrafish were randomly selected from each experimental group and placed under a fluorescence microscope for photography. The data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software, and the fluorescence intensity of zebrafish yolk sac fat was analyzed. The statistical analysis results of this indicator were used to evaluate the efficacy of the sample in promoting lipolysis. The statistical analysis results were expressed as mean±SE. SPSS26.0 software was used for statistical analysis between the two groups, and p<0.05 indicated that the difference was statistically significant.

[0032] 3. Experimental results

[0033] 3.1 Maximum detectable concentration (MTC) determination results.

[0034] The MTC test results are shown in Table 1 below:

[0035] Table 1

[0036]

[0037] The zebrafish in the experimental group were similar to those in the normal control group when the white kidney bean extract was in the intervention range of 12.5 to 200 μg / mL. Therefore, under the experimental conditions, the MTC of the white kidney bean extract in promoting lipolysis was 200 μg / mL. 5 CFU / mL~1×10 8CFU / mL intervention dose range, the state of zebrafish in the experimental group was similar to that of the normal control group, while 1×10 9 CFU / mL group, 5 zebrafish died, with a mortality rate of 17%. Therefore, the MTC of MN-Gup powder in promoting lipolysis under the experimental conditions was 1×10 8 CFU / mL.

[0038] 3.2 White kidney bean extract promotes lipolysis

[0039] Nile Red is a fluorescent hydrophobic probe that can indicate neutral fats such as triglycerides as golden yellow and phospholipids as orange-red under the conditions of excitation wavelength of 450-500nm and emission wavelength>520nm. The specific in vivo fluorescent dye Nile Red can be used to stain and mark fats, and when observed under a fluorescence microscope, a decrease in fluorescence intensity indicates the promotion of fat decomposition.

[0040] The experimental results of white kidney bean extract promoting fat decomposition are as follows Figure 1 As shown, Figure 1 * indicates significant difference compared with the normal group (P<0.05). Figure 1 It can be seen that the fluorescence intensity of yolk sac fat in the normal group was 3.76±240,000 pixels. The fluorescence intensity of yolk sac fat in the low, medium and high dose groups of white kidney bean extract was 2.85±310,000 pixels, 2.71±240,000 pixels and 2.35±170,000 pixels, respectively, which were significantly lower than those in the normal group, indicating that low, medium and high doses of white kidney bean extract all have the effect of promoting lipolysis.

[0041] Example 2

[0042] Application of a white kidney bean composition in preparing a product promoting fat decomposition.

[0043] The composition of white kidney bean extract of the present invention comprises white kidney bean extract and animal Bifidobacterium lactis subspecies MN-Gup in different proportions in compositions 1-5; the animal Bifidobacterium lactis subspecies MN-Gup has a preservation number of CGMCC No.15578, which is purchased from Mengniu High-Tech Dairy (Beijing) Co., Ltd., and its source is the Bama Longevity Village in Guangxi, the "World Longevity Hometown", and the conventional viable bacteria count of MN-Gup bacterial powder is 1 to 5×10 11 CFU / g, the viable bacterial count of the MN-Gup powder used in this example is 2×10 11 CFU / g. The ratio of white kidney bean extract to MN-Gup powder in composition 1 is 1:0.05, the ratio in composition 2 is 1:1, the ratio in composition 3 is 1:20, the ratio in composition 4 is 50:1, and the ratio in composition 5 is 1:50.

[0044] Application effect detection of promoting fat decomposition:

[0045] 1. Experimental Animals

[0046] Zebrafish were raised in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / LCaCO3).

[0047] 2. Detection method

[0048] The composition of white kidney bean extract is a combination of white kidney bean extract and animal Bifidobacterium lactis subspecies MN-Gup. The present invention uses compositions with different compounding ratios to verify the effects of the compositions in promoting fat decomposition. Specifically, compound group 1 (FP1) uses composition 1, compound group 2 (FP2) uses composition 2, compound group 3 (FP3) uses composition 3, compound group 4 (FP4) uses composition 4, and compound group 5 (FP5) uses composition 5.

[0049] 2dpf melanin allele mutant zebrafish (albino) were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well. The samples of the combination of FP1-FP5 were given in water, and the total intervention dose of the samples was 200μg / mL. At the same time, a normal control group (NC), a white kidney bean extract group (BYD), and an animal Bifidobacterium lactis subsp. MN-Gup group (MN-Gup) were set up. The intervention doses of BYD and MN-Gup were also 200μg / mL, and the volume of each well was 3mL. After 1 day of treatment at 28℃, each experimental group was given Nile red dye in water, and the treatment was continued at 28℃ until 4dpf. Ten zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software, and the fluorescence intensity of zebrafish yolk sac fat was analyzed. The statistical analysis results of this indicator were used to evaluate the efficacy of the samples in promoting lipolysis. The statistical processing results are expressed as mean±SE. SPSS26.0 software was used for statistical analysis between the two groups, and p<0.05 indicated that the difference was statistically significant.

[0050] 3. Experimental results

[0051] The experimental results of FP1-FP5 are as follows Figure 2 As shown, Figure 2 In the table, *: significant difference compared with the normal control group (NC) (P < 0.05); #: significant difference compared with the white kidney bean extract group (BYD) (P < 0.05); &: significant difference compared with the MN-Gup group (P < 0.05).

[0052] Figure 2The fluorescence intensity of yolk sac fat in the normal control group (NC) was 4.75±230,000 pixels, and the fluorescence intensity of yolk sac fat in the white kidney bean extract and MN-Gup was 3.27±220,000 pixels and 3.45±330,000 pixels, respectively, which were significantly lower than those in the normal control group, indicating that both of them had the effect of promoting lipolysis at an intervention concentration of 200 μg / mL. The fluorescence intensity of yolk sac fat in the FP1-FP5 groups was 2.70±130,000 pixels, 2.58±150,000 pixels, 2.77±140,000 pixels, 3.24±160,000 pixels, and 3.52±200,000 pixels, respectively, which were significantly lower than those in the normal control group, indicating that at an intervention concentration of 200 μg / mL, the compound groups all had the effect of promoting lipolysis. Among them, the fluorescence intensity of yolk sac fat in groups FP1 to FP3 was 2.70±130,000 pixels, 2.58±150,000 pixels, and 2.77±140,000 pixels, respectively, which were significantly lower than those in the normal control group, and significantly lower than those in the white kidney bean extract group and the MN-Gup group. This indicates that the ratio of white kidney bean extract to MN-Gup is in the range of 1:0.05 to 20, which has a synergistic effect in promoting lipolysis. This proves that white kidney bean extract and MN-Gup have a synergistic effect in promoting lipolysis.

[0053] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. Application of white kidney bean extract in the preparation of products promoting fat decomposition.

2. Application of a composition of white kidney bean extract in the preparation of a product for promoting fat decomposition.

3. The use according to claim 2, characterized in that: The composition comprises white kidney bean extract and animal bifidobacterium lactis subspecies MN-Gup.

4. The use according to claim 3, characterized in that: The mass ratio of the white kidney bean extract to animal Bifidobacterium lactis subspecies MN-Gup is 1:(0.05-20).

5. The use according to claim 4, characterized in that: The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 CFU / g and above.

6. The use according to claim 5, characterized in that: The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 ~500×10 9 CFU / g.

7. A composition for promoting fat decomposition, characterized in that: Includes white kidney bean extract and Bifidobacterium animalis subsp. lactis MN-Gup.

8. The composition according to claim 7, characterized in that The mass ratio of the white kidney bean extract to animal Bifidobacterium lactis subspecies MN-Gup is 1:(0.05-20).

9. The composition according to claim 8, characterized in that The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 CFU / g and above.

10. The composition according to claim 9, characterized in that The viable bacterial count of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 4×10 9 ~500×10 9 CFU / g.

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