Traditional Chinese medicine compound for treating blood stasis arthralgia type osteoarthritis

Abstract

A traditional Chinese medicine compound for treating blood stasis arthralgia type osteoarthritis belongs to the field of traditional Chinese medicine preparations and comprises 8 g of polygonum cuspidatum, 10 g of rhizoma sparganii, 10 g of turmeric, 10 g of motherwort, 10 g of artemisia anomala, 10 g of rhizoma curculiginis, 10 g of ramulus mori, 10 g of lycopodium clavatum, 10 g of bungarus parvus, 8 g of radix angelicae, 10 g of codonopsis pilosula, 10 g of semen coicis, 8 g of scutellaria baicalensis, 8 g of honeysuckle and 8 g of fructus forsythiae. All the medicines are put into a non-woven fabric bag to be sealed and put into a container, and water is added for boiling. Effective components of the traditional Chinese medicines are separated out by heating to prepare the fumigation and washing formula, and the effects of promoting blood circulation to remove blood stasis and activating meridians to stop pain are achieved when the fumigation and washing formula is used.

Description

Technical Field

[0001] The invention belongs to the field of Chinese medicine preparations, and in particular relates to a Chinese medicine compound for treating blood stasis and arthritis type osteoarthritis. Background Art

[0002] Osteoarthritis is a common degenerative joint disease with complex and diverse pathogenesis, among which blood stasis and stagnation type is particularly difficult. Patients often present with joint pain, swelling, limited mobility, and obvious blood stasis symptoms, such as dark purple tongue and sluggish pulse. According to TCM theory, blood stasis and stagnation, poor blood circulation, are the root causes of pain and dysfunction. As a kind of TCM external treatment, Chinese medicine foot bath therapy uses the thermal effect of the medicine and the penetration of the skin and meridians to allow the medicine to reach the diseased area directly, achieving the purpose of promoting blood circulation, removing blood stasis, unblocking meridians and relieving pain. The Chinese medicine fumigation and washing compound for blood stasis and stagnation type osteoarthritis is based on the principle of TCM syndrome differentiation and treatment, and is composed of a variety of Chinese medicines with the effects of promoting blood circulation, removing blood stasis, warming the meridians and dispelling cold, and removing wind and dampness. It aims to improve local blood circulation in the joints, promote inflammation absorption, and relieve pain and dysfunction through local treatment. Summary of the invention

[0003] The object of the present invention is to provide a traditional Chinese medicine compound for effectively treating blood stasis and obstruction type osteoarthritis. The traditional Chinese medicine compound comprises polygonum cuspidatum, motherwort, turmeric, aconite, three-edged sword, curculigo, mulberry branch, herba schizonepetae, scutellariae, white snakehead, angelica dahurica, codonopsis pilosula, coix seed, scutellariae baicalensis, honeysuckle and forsythiae. The effective components of the traditional Chinese medicine are extracted by heating to prepare a fumigation and washing formula. When the fumigation and washing is used, the effects of promoting blood circulation, removing blood stasis, unblocking meridians and relieving pain can be achieved.

[0004] The technical solution adopted by the present invention is:

[0005] A traditional Chinese medicine compound for treating blood stasis and obstruction type osteoarthritis is prepared from the following traditional Chinese medicine raw materials by weight: 8g of Polygonum cuspidatum, 10g of Trigonum multiflorum, 10g of Curcuma longa, 10g of Leonurus japonicus, 10g of Achyranthes bidentata, 10g of Curculigo orchioides, 10g of Morus alba, 10g of Herba Semen ...

[0006] Polygonum cuspidatum, Leonurus japonicus, Curcuma longa, Liuji Nu, and Tripterygium wilfordii are all blood-activating and blood-stasis-removing drugs, and play a role in blood-stasis-removing and pain-relieving effects. Polydatin can activate the autophagic flux in chondrocytes through the mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase / protein kinase B (PI3K / Akt) signaling pathways, thereby playing a cartilage-protective role in osteoarthritis. Neutrophil extracellular reticular trap (NET) formation is a potential mechanism for the treatment of arthritis. Polydatin can significantly inhibit the formation of NET and slow down the development of arthritis in mice with collagen-induced arthritis. Leonurus japonicus can inhibit the activation of NOD-like receptor pyrin domain-related protein 3 (NLRP3) inflammasome by upregulating the level of mammalian rapamycin target protein (mTOR) to regulate the phosphorylation-mediated autophagy of related proteins, showing anti-inflammatory effects. The anti-inflammatory efficacy of curcumin is comparable to that of steroidal and nonsteroidal anti-inflammatory drugs. Its mechanism is to inhibit the occurrence of inflammatory response by inhibiting the activity of active enzymes and reactive oxygen cluster enzymes that promote inflammatory response, reducing the activity of macrophages and the expression of protein kinases. Curcumin can inhibit the growth of fibroblasts by inhibiting the expression of cyclooxygenase (COX) and lipoxygenase (LOX) to promote cell apoptosis, thereby inhibiting the occurrence and development of inflammation. It can also play a protective role in osteoarthritis chondrocytes through the tyrosine protein kinase-2 / transcription activator-3 (JAK-2 / STAT-3) signaling pathway. The total flavonoids of Liujinu may be the material basis of the anti-inflammatory effect of Liujinu. The ethyl acetate part of Liujinu is one of the anti-inflammatory active components of Liujinu. The total flavonoids enriched in this way inhibit the nitrite content in the cell supernatant in a dose-dependent manner, and partially achieve anti-inflammatory effects by inhibiting the expression of NOS and COX-2 genes and proteins through the extracellular signal-regulated kinase signaling pathway in mitogen-activated protein kinase. Sanleng Pills can significantly prolong the pain response time of mice, significantly reduce the number of body twisting of mice, and reduce the swelling of rat paws and mouse ears.

[0007] Curculigo, Morus alba, and Herba Artemisiae Rhizoma are all drugs for removing dampness and unblocking meridians. Herba Artemisiae Rhizoma and Radix Angelicae Dahuricae also have the function of unblocking meridians, which can relieve the symptoms of arthritis and difficulty in walking. Curculigo polysaccharide isolated from Curculigo can enhance the phagocytic function of mouse macrophages in a dose-dependent manner, and can exert anti-inflammatory effects by inhibiting the release of inflammatory cytokines, downregulating JAK / STAT signaling pathway proteins, and increasing the expression of nuclear factor κB (NF-κB). Mulberry polysaccharide (MFP) can regulate the secretion of various cytokines involved in inflammatory response, and thus also has a certain anti-inflammatory effect. Sanggenone B and Albenol B in Morus alba can reduce the production of pro-inflammatory cytokines such as TNF-α and IL-6 in RAW264.7 macrophages induced by lipopolysaccharide in vitro, and reduce the expression of inducible iNOS and COX-2, showing a certain anti-inflammatory effect. The anti-inflammatory effect of total alkaloids of Herba Lycopodii showed a dose-dependent characteristic, and the improvement of adjuvant arthritis in model rats in the medium and high dose groups was more significant, indicating that Herba Lycopodii had a good anti-inflammatory effect. The alkaloid components of Herba Lycopodii could significantly reduce the swelling of joints in rats with CFA-induced arthritis and improve their synovial lesions, which may be related to the intervention of IL-1β and TNF-α levels. Herba Lycopodii had a significant inhibitory effect on xylene-induced auricular inflammation in mice and egg white-induced foot swelling in rats and mice. Pentenyloxyfuran in Angelica dahurica could effectively block the inflammatory response induced by endotoxin. Angelica dahurica extract could significantly reduce the swelling of the left ankle joint induced by MSU in rats, improve the inflammatory infiltration in the synovial tissue, and significantly reduce the protein and mRNA expression of NLRP3, IL-1β, caspase-1, IL-1R1, IL-6, and TNF-α in the synovial tissue, thereby reducing the inflammatory response.

[0008] Codonopsis and coix seed both have the function of strengthening the spleen, which helps to strengthen the spleen and stomach and enhance the body's immune function. Codonopsis can promote T lymphocyte proliferation, regulate the secretion of inflammatory factors by T lymphocytes and macrophages, and maintain the dynamic balance of immune cells, thereby enhancing the body's immune function. Codonopsis polysaccharides and codonopsis saponins can inhibit inflammatory responses by affecting the upstream and downstream factors of the NF-κB signaling pathway. Scutellaria baicalensis, honeysuckle, and forsythia have the function of clearing heat and help relieve joint redness, swelling, heat, and pain. Baicalin can regulate immune function through the Akt signaling pathway, p53 signaling pathway, MAPK signaling pathway, T cell receptor pathway, etc. Honeysuckle has antipyretic and anti-inflammatory effects and can significantly improve the immune function of immunocompromised mice. Lignans B and C in forsythia leaves have anti-inflammatory activity against LPS-induced macrophages. Forsythiaside A inhibits the expression of TNF-α, IL-1β, NO and PGE2 in cells by inhibiting NF-κB and activating the Nrf2 / HO-1 pathway, thereby suppressing the inflammatory response induced by lipopolysaccharide (LPS) in cells.

[0009] In the foot bath fumigation and washing prescription proposed by the present invention, Trigonum multiflorum and Curcuma longa can promote blood circulation and qi, have strong stasis-removing power, and can also promote qi circulation and relieve pain, promote blood circulation and activate collaterals. The two play the role of monarch medicine in the prescription, Leonurus japonicus can promote blood circulation, promote diuresis and relieve swelling, and can relieve joint swelling and pain caused by arthritis, Herba Ligustici ammiguli is warm in nature and bitter in taste, and can enter the heart meridian, spleen meridian and liver meridian, Polygonum cuspidatum is slightly bitter in taste and slightly cold in nature, and belongs to the liver, gallbladder and lung meridians, and both can disperse blood stasis and relieve pain, and help the monarch medicine to promote blood circulation and remove blood stasis, promote blood circulation and relieve pain, and are altogether ministerial medicines. Morus twig mainly belongs to the liver meridian, dispels rheumatism, and benefits joints; Herba Artemisiae Rhizoma dispels wind and dampness, promotes blood circulation and activates collaterals; Curculigo orchioides nourishes kidney yang, strengthens tendons and bones, and dispels cold and dampness; Herba Artemisiae Rhizoma dispels wind, promotes collaterals and stops spasm; Angelica dahurica dispels cold, dispels wind and relieves pain; they are altogether adjuvant medicines, and help the monarch medicine to dispel wind and dampness, promote blood circulation and relieve pain. Arthritis damages the liver and kidneys over time, and is easily affected by wind, cold and dampness, which damages the spleen and stomach. Therefore, Codonopsis pilosula and Coix seeds are added to help the spleen and stomach to transport and transform, invigorate the spleen and remove dampness, and relieve pain. If the spleen and stomach transport and transform poorly, the water metabolism will be impaired, and dampness will form over time. In addition, blood stasis will block the collaterals, which will easily cause depression and heat, resulting in joint swelling, heat and pain. Therefore, Scutellaria baicalensis, honeysuckle and forsythia are added to remove heat. The combination of all the medicines in the prescription can achieve the effect of promoting blood circulation, removing blood stasis, and relieving pain. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 Comparison of joint indexes between the two groups before and after treatment (*p<0.05, **p<0.01).

[0011] Figure 2 Comparison of inflammatory factors between the two groups before and after treatment (*p<0.05, **p<0.01). DETAILED DESCRIPTION

[0012] The following is a further detailed description of a Chinese medicinal compound for treating blood stasis and obstruction type osteoarthritis of the present invention in conjunction with the accompanying drawings and examples.

[0013] Example 1

[0014] A Chinese medicinal compound for treating blood stasis and arthralgia type osteoarthritis is prepared from the following Chinese medicinal raw materials by weight:

[0015] Polygonum cuspidatum 8g, Trigonum multiflorum 10g, Curcuma longa 10g, Leonurus japonicus 10g, Liu Jinnu 10g, Curculigo orchioides 10g, Morus alba 10g, Herba schizonepetae 10g, Herba schizonepetae 10g, Herba schizonepetae 10g, Herba schizonepetae 10g, Radix Angelicae Dahuricae 8g, Codonopsis pilosulae 10g, Semen Coicis 10g, Radix Scutellariae 8g, Flos Lonicerae 8g, Fructus Forsythiae 8g. Put all the drugs into a non-woven bag, seal it, put it into a container, add water and boil it to obtain the foot bath fumigation recipe.

[0016] Example 2

[0017] In this experiment, an osteoarthritis mouse model was prepared to study the effect of Chinese medicine foot bath fumigation on the arthritis index, inflammatory factors IL-10 and TGF-β of the model mice.

[0018] 1. Materials and Methods

[0019] 1.1 Animals

[0020] Twenty SPF female mice, aged 42 to 56 days, were purchased from Anhui Experimental Animal Research Center. Complete Freund's adjuvant-induced osteoarthritis model was established.

[0021] 1.2 Chinese medicine fumigation and washing prescription

[0022] Chinese medicine fumigation formula: Polygonum cuspidatum 8g, Trigonum spicatum 10g, Curcuma longa 10g, Leonurus japonicus 10g, Liu Jinnu 10g, Curculigo orchioides 10g, Morus alba 10g, Herba schizonepetae 10g, Herba schizonepetae 10g, Herba schizonepetae 10g, Herba schizonepetae 10g, Herba schizonepetae 8g, Herba schizonepetae 10g, Herba schizonepetae 8g, Herba schizonepetae 10g, Herba schizonepetae 8g, Herba schizonepetae 8g. This is the agreed formula of the Department of Rheumatology of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, and the Chinese medicinal materials used are provided by the Chinese medicine pharmacy of the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine. All drugs are sealed in a non-woven bag, placed in a container, and boiled with water.

[0023] 1.3 Methods

[0024] 1.3.1 Modeling

[0025] After each mouse was anesthetized with isoflurane, 20 μL of complete Freund's adjuvant was injected between the second and third webs of the left toes. The swelling of the left foot and the decrease in the mechanical and thermal pain thresholds were considered as the signs of successful modeling. The mice in the sham operation group were operated in the same way as above and injected with an equal volume of normal saline. After successful modeling, they were randomly divided into the control group and the study group, with 10 mice in each group.

[0026] 1.3.2 Administration

[0027] The control group was given celecoxib combined with tofacitinib, celecoxib 0.2g / time, once a day by gavage (Qingdao Baiyang Pharmaceutical Co., Ltd., National Medicine Standard: H20203325); tofacitinib 5mg / time, once a day by gavage (Zhengda Tianqing Pharmaceutical Group, National Medicine Standard: H20193281). The research group was given a high-concentration Chinese medicine fumigation formula on the basis of the control group, once a day for 4 weeks. The various indicators of the rats were observed every day for four weeks.

[0028] 1.4 Observation indicators and detection methods

[0029] (1) The arthritis index was used to evaluate the degree of arthritis in mice before and after treatment. The full score was 100, and the higher the score, the better.

[0030] (2) Compare the levels of inflammatory factors IL-10 and TGF-β between the two groups before and after treatment.

[0031] 2. Results

[0032] 2.1 Arthritis Index

[0033] The arthritis index of mice in each group was compared before and after treatment. The arthritis index of the control group before treatment was (14.26±2.59) points, and the arthritis index of the research group before treatment was (14.56±2.10) points. The arthritis index of the control group after treatment was (10.68±1.52) points, and the arthritis index of the research group after treatment was (9.14±1.84) points. The arthritis index of the control group after treatment was significantly lower than that before treatment (p<0.05), the arthritis index of the research group after treatment was significantly lower than that before treatment (p<0.01), and the arthritis index of the research group after treatment was significantly lower than that of the control group after treatment (p<0.01) (the results are shown in Figure 1 shown).

[0034] 2.2 Comparison of inflammatory factors IL-10 and TGF-β

[0035] The levels of IL-10 and TGF-β in each group of mice were compared before and after treatment. The levels of IL-10 and TGF-β in the control group before treatment were (19.13±4.35) ng / L and (24.8±3.93) ng / L, respectively; the levels of IL-10 and TGF-β in the study group before treatment were (19.72±3.79) ng / L and (23.14±1.62) ng / L, respectively. The levels of IL-10 and TGF-β in the control group after treatment were (12.67±1.64) ng / L and (18.31±3.35) ng / L, respectively; the levels of IL-10 and TGF-β in the study group after treatment were (11.91±1.38) ng / L and (14.78±1.54) ng / L, respectively. After treatment, IL-10 and TGF-β in the control group were significantly lower than those before treatment (p < 0.05), and IL-10 and TGF-β in the study group were significantly lower than those before treatment (p < 0.01). Figure 2 shown).

[0036] The advantage of Chinese medicine foot bath fumigation formula in treating blood stasis and obstruction type osteoarthritis lies in its unique treatment method and comprehensive treatment effect. It not only enables the drug to quickly reach the diseased area and play a therapeutic role through the direct action of the drug and the penetration of heat; it also focuses on overall regulation and syndrome differentiation and treatment, aiming to fundamentally improve the patient's physical condition and condition.

[0037] In addition, Chinese medicine foot bath fumigation therapy has the advantages of simple operation, few side effects, and easy acceptance by patients, so it has a wide range of application prospects. This experiment found that Chinese medicine foot bath fumigation therapy can help osteoarthritis mice arthritis index, inflammatory factors IL-10, TGF-β to be significantly improved, indicating that the Chinese medicine foot bath fumigation can improve the joint inflammation of mice by reducing inflammatory factors.

[0038] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A Chinese medicinal compound for treating blood stasis type osteoarthritis, characterized in that: The foot bath formula is prepared from the following Chinese medicinal raw materials by weight: 8g of Polygonum cuspidatum, 10g of Trigonum multiflorum, 10g of Curcuma longa, 10g of Leonurus japonicus, 10g of Achyranthes bidentata, 10g of Curculigo orchioides, 10g of Morus alba, 10g of Herba Semen Semen Semen Semen, 10g of Herba Schizonepetae, 10g of Agkistrodon acutus, 8g of Angelica dahurica, 10g of Codonopsis pilosula, 10g of Coix lachryma-jobi, 8g of Scutellaria baicalensis, 8g of Honeysuckle, and 8g of Forsythia suspensa; all the drugs are put into a non-woven bag, sealed, put into a container, add water and boil to obtain a foot bath fumigation recipe.

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