Preparation method of special cattle hoof medicated bath disinfectant for dairy farm

Abstract

The preparation method of the special cattle hoof medicated bath disinfectant for the dairy farm comprises the following steps: S01, adding zinc pyrrolidone carboxylate into a micro-nano zinc solution, heating to 40-60 DEG C, stirring for 0.5-2.5 hours, adding bovine bone collagen peptide powder, continuously stirring for 0.5-1.5 hours, cooling and filtering to obtain a precursor solution; s02, adding a mixture of sodium iodide and potassium iodate into the precursor solution, adding glycerol, a surfactant and deionized water, stirring for 2.5-4.5 hours at the temperature of 60-80 DEG C, and cooling to obtain a medicated bath disinfectant for the cattle hoof; the selected materials are green, environment-friendly, non-toxic and non-irritant, and the disinfectant belongs to the environment-friendly medicated bath disinfectant for the cattle hoof; a better prevention effect is achieved on hoof warts, interdigital dermatitis and foot rot; the product system is uniform and stable, the water solubility is good, and the dilution ratio is large; the health condition and the production efficiency of dairy cows are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a method for preparing a special cattle hoof medicinal bath disinfectant for dairy farms. Background Art

[0002] In daily operation of dairy farms, cow hooves are often injured by foreign objects, such as punctures, contusions or accidental injuries, dermal inflammation, and secondary infection with bacteria such as necrotic bacillus, pyogenic bacillus, streptococci, and nodular clostridium. In order to prevent cow hooves from suppuration, disinfection is performed before cows enter the milking parlor.

[0003] The harm of cow hoof suppuration is mainly reflected in the following aspects:

[0004] 1. Pain: Hoof suppuration can cause local pain, affecting the cow's walking and standing. In severe cases, it can cause the cow to be unable to walk normally.

[0005] 2. Spread of infection: The festering cow hooves are susceptible to bacterial infection. If not treated in time, the bacteria may spread to other parts through the blood, causing systemic infection.

[0006] 3. Hoof injury: suppurative hoof may damage the hoof structure, causing the hoof to deform or fall off, affecting the cow's walking and standing.

[0007] 4. Decreased appetite: Due to the effects of pain and infection, cattle may have a decreased appetite, leading to malnutrition and weight loss.

[0008] At present, various hoof medicated baths on the market have various problems. Some have poor effects, some use materials that are not environmentally friendly, have side effects, and some bring other hazards, which do not play a fundamental role in the harm of hoof suppuration in dairy cows. The present invention can not only solve the harm of hoof suppuration, but also has environmentally friendly materials and a repairing effect. Therefore, before dairy cows enter the milking hall, they must be quickly sterilized and have a lasting antibacterial effect to ensure that the cow's hoof greatly reduces bacterial infection and ensures the health of dairy cows. Summary of the invention

[0009] In order to solve the problem that cattle hooves are easily injured and infected with bacteria, leading to suppuration, the present invention discloses a cattle hoof disinfectant that can quickly kill bacteria and has a long-lasting antibacterial effect, and is widely used in pastures.

[0010] To achieve the above object, the technical solution of the present invention is as follows:

[0011] The invention provides a method for preparing a special cattle hoof medicinal bath disinfectant for dairy farms, comprising the following steps:

[0012] Add zinc pyrrolidone carboxylate to the S01 micro-nano zinc solution, heat to 40°C-60°C, stir for 0.5-2.5h, add bovine collagen peptide powder, continue stirring for 0.5-1.5h, cool and filter to obtain a precursor solution;

[0013] A mixture of sodium iodide and potassium iodate is added to the S02 precursor solution, and glycerol, a surfactant, and deionized water are added. The mixture is stirred at a temperature of 60-80°C for 2.5-4.5 hours, and then cooled to obtain a cattle hoof medicated bath disinfectant.

[0014] Furthermore, in step S01, the mass ratio of micro-nano zinc to zinc pyrrolidone carboxylate is 1:(2-5).

[0015] Furthermore, in step S01, the mass ratio of micro-nano zinc to bovine collagen peptide powder is 1:(0.5-2).

[0016] Furthermore, in step S02, the mass ratio of sodium iodide to potassium iodide is 1:1.

[0017] Furthermore, in step S02, the mass ratio of the precursor solution to sodium iodide is 1:(0.01-0.1).

[0018] Furthermore, in step S02, the mass ratio of sodium iodide, glycerol, surfactant, and deionized water is 1:(0.5-3):(0.3-5):(20-100).

[0019] Furthermore, the surfactant is one or more of betaine, polyoxyethylene stearyl alcohol ether, sodium lauryl sulfate, sodium hexadecyl sulfate, and sodium octadecyl sulfate.

[0020] The addition of zinc pyrrolidone carboxylate in S01 of the present invention, stirring for 0.5-2.5h, plays the role of a catalyst at low temperature (40°C-60°C), has better selectivity and stability, and makes the prepared precursor solution more stable. The addition of bovine collagen peptide powder, stirring for 0.5-1.5h under 40°C-60°C, can form a precursor solution of multi-component coordination compounds with micro-nano zinc, strengthen the disinfection function, and have a better preventive effect on hoof warts, interdigital dermatitis and hoof rot. The addition of a mixture of sodium iodide and potassium iodate in the S02 precursor solution can play a synergistic role with the precursor solution, reduce the spread of infection, and prevent the occurrence of hoof rot. Glycerol can strengthen the effect of the precursor solution and reduce the effect of hoof damage. The addition of surfactants can make the entire system more compatible and more stable. The addition of deionized water is to promote the reaction and adjust the effective content of the product.

[0021] The beneficial effects of the present invention are:

[0022] The invention is environmentally friendly and non-toxic, non-irritating, and belongs to an environmentally friendly cattle hoof medicinal bath disinfectant;

[0023] The invention has a better preventive effect on hoof warts, interdigital dermatitis and hoof rot;

[0024] The product system of the present invention is uniform and stable, has good water solubility and a large dilution multiple;

[0025] The invention improves the health status and production efficiency of dairy cows. DETAILED DESCRIPTION

[0026] The present invention is further explained below in conjunction with the attached table and specific implementation methods. It should be understood that the following specific implementation methods are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0027] Example 1

[0028] S01: Add 3g of zinc pyrrolidone carboxylate to 300ml of 5000ppm micro-nano zinc, heat to 40°C in a beaker, stir for 0.5h, add 0.75g of bovine collagen peptide powder, continue stirring for 0.5h, cool and filter to obtain a precursor solution;

[0029] S02: Take 250 ml of S01 precursor solution, add 5 g of a mixture of sodium iodide and potassium iodate (1:1), add 1.25 g of glycerol, add 0.75 g of betaine, add 50 ml of deionized water, stir at 60°C for 2.5 hours, and then cool to obtain a cattle hoof medicated bath disinfectant.

[0030] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0031] The prepared cattle hoof medicated bath disinfectant was tested, and the test process and results are as follows:

[0032] 1. Equipment

[0033] 1. Sample: Cattle hoof disinfectant (diluted 60 times);

[0034] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0035] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0036] 4. Organic interferences: 3% bovine serum albumin;

[0037] 5. Constant temperature incubator (model: 404L);

[0038] 6. Culture medium: ordinary nutrient agar;

[0039] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0040] 8. Standard hard water: hardness 342 mg / L.

[0041] 2. Methods

[0042] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0043] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0044] Cattle hoof disinfectant bath: water 1:60 Action time: 2min;

[0045] The test temperature was 20℃ and the experiment was repeated 3 times.

[0046] 3. Test results

[0047] The test results are shown in Table 1

[0048] Table 1 Detection results of cattle hoof disinfectant bath

[0049]

[0050] The rest is the same as in Example 1, except that zinc pyrrolidone carboxylate is not added, and the other components and processes remain unchanged. The test results of the prepared cattle hoof medicated bath disinfectant are shown in Table 2:

[0051] Table 2 Detection results of cattle hoof disinfectant bath

[0052]

[0053] Example 2

[0054] S01: Add 7.5g of zinc pyrrolidone carboxylate to 300ml of 5000ppm micro-nano zinc, heat to 60℃ in a beaker and stir for 2.5h, add 3g of bovine collagen peptide powder, continue stirring for 1.5h, cool and filter to obtain a precursor solution;

[0055] S02: Take 250 ml of S01 precursor liquid, add 50 g of a mixture of sodium iodide and potassium iodate (1:1), add 12.5 g of glycerol, add 3.5 g of betaine, add 3.5 g of polyoxyethylene stearyl ether, add 0.5 g of sodium lauryl sulfate, add 2500 ml of deionized water, stir at 80°C for 4.5 hours, and then cool to obtain a cattle hoof medicated bath disinfectant.

[0056] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0057] The prepared cattle hoof medicated bath disinfectant was tested, and the test process and results are as follows:

[0058] 1. Equipment

[0059] 1. Sample: Cattle hoof disinfectant (diluted 80 times);

[0060] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0061] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0062] 4. Organic interferences: 3% bovine serum albumin;

[0063] 5. Constant temperature incubator (model: 404L);

[0064] 6. Culture medium: ordinary nutrient agar;

[0065] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0066] 8. Standard hard water: hardness 342 mg / L.

[0067] 2. Methods

[0068] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0069] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0070] Concentration of cattle hoof disinfectant bath: 1:80 Action time: 2min;

[0071] The test temperature was 20°C and the experiment was repeated 3 times.

[0072] 3. Test results

[0073] The test results are shown in Table 3

[0074] Table 3 Cattle hoof disinfectant test results

[0075]

[0076] The other components are the same as those in Example 2, except that no bovine collagen peptide powder is added. The other components and processes remain unchanged. The test results of the prepared bovine hoof medicated bath disinfectant are shown in Table 4.

[0077] Table 4 Cattle hoof disinfectant bath test results

[0078]

[0079] Example 3

[0080] S01: Add 4.5g of zinc pyrrolidone carboxylate to 300ml of 5000ppm micro-nano zinc, heat to 60℃ in a beaker and stir for 1.5h, add 1.5g of bovine collagen peptide powder, continue stirring for 1h, cool and filter to obtain a precursor solution;

[0081] S02: Take 250 ml of S01 precursor liquid, add 10 g of a mixture of sodium iodide and potassium iodate (1:1), add 2.8 g of glycerol, add 1.5 g of betaine, add 2.5 g of polyoxyethylene stearyl ether, add 0.2 g of sodium lauryl sulfate, add 300 ml of deionized water, stir at 70°C for 2.8 hours, and then cool to obtain cattle hoof medicated bath disinfectant.

[0082] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0083] The prepared cattle hoof medicated bath disinfectant was tested, and the test process and results are as follows:

[0084] 1. Equipment

[0085] 1. Sample: Cattle hoof disinfectant (diluted 80 times);

[0086] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0087] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0088] 4. Organic interferences: 3% bovine serum albumin;

[0089] 5. Constant temperature incubator (model: 404L);

[0090] 6. Culture medium: ordinary nutrient agar;

[0091] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0092] 8. Standard hard water: hardness 342 mg / L.

[0093] 2. Methods

[0094] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0095] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0096] Concentration of cattle hoof disinfectant bath: 1:80 Action time: 2min;

[0097] The test temperature was 20°C and the experiment was repeated 3 times.

[0098] 3. Test results

[0099] The test results are shown in Table 5.

[0100] Table 5 Cattle hoof disinfectant bath test results

[0101]

[0102] The rest is the same as in Example 3, except that sodium iodide and potassium iodate are not added, and the other components and processes remain unchanged. The test results of the prepared cattle hoof medicated bath disinfectant are shown in Table 6:

[0103] Table 6 Cattle hoof disinfectant bath test results

[0104]

[0105] Example 4

[0106] S01: Add 5.5g of zinc pyrrolidone carboxylate to 300ml of 5000ppm micro-nano zinc, heat to 55℃ in a beaker and stir for 1.2h, add 1.8g of bovine collagen peptide powder, continue stirring for 1h, cool and filter to obtain a precursor solution;

[0107] S02: Take 250 ml of S01 precursor liquid, add 20 g of a mixture of sodium iodide and potassium iodate (1:1), add 12 g of glycerol, add 3.5 g of betaine, add 3.5 g of polyoxyethylene stearyl ether, add 1.2 g of sodium lauryl sulfate, add 800 ml of deionized water, stir at 65 ° C for 3 hours, and then cool to obtain cattle hoof medicated bath disinfectant.

[0108] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0109] The prepared cattle hoof medicated bath disinfectant was tested, and the test process and results are as follows:

[0110] 1. Equipment

[0111] 1. Sample: Cattle hoof disinfectant (diluted 60 times);

[0112] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0113] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0114] 4. Organic interferences: 3% bovine serum albumin;

[0115] 5. Constant temperature incubator (model: 404L);

[0116] 6. Culture medium: ordinary nutrient agar;

[0117] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0118] 8. Standard hard water: hardness 342 mg / L.

[0119] 2. Methods

[0120] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0121] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0122] Cattle hoof disinfectant concentration: 1:60 Action time: 2min;

[0123] The test temperature was 20°C and the experiment was repeated 3 times.

[0124] 3. Test results

[0125] The test results are shown in Table 7

[0126] Table 7 Cattle hoof disinfectant bath test results

[0127]

[0128] The rest is the same as in Example 4, except that glycerol is not added, and other components and processes remain unchanged. The test results of the prepared cattle hoof medicated bath disinfectant are shown in Table 8:

[0129] Table 8 Cattle Hoof Bath Disinfectant Test Results

[0130]

[0131] Example 5

[0132] S01: Add 3.5g of zinc pyrrolidone carboxylate to 300ml of 5000ppm micro-nano zinc, heat to 50℃ in a beaker and stir for 2h, add 2.6g of bovine collagen peptide powder, continue stirring for 1.2h, cool and filter to obtain a precursor solution;

[0133] S02: Take 250 ml of S01 precursor liquid, add 30 g of a mixture of sodium iodide and potassium iodate (1:1), add 26 g of glycerol, add 1.5 g of betaine, add 4.5 g of polyoxyethylene stearyl ether, add 2.2 g of sodium lauryl sulfate, add 1200 ml of deionized water, stir at 75°C for 3.5 hours, and then cool to obtain cattle hoof medicated bath disinfectant.

[0134] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0135] The prepared cattle hoof medicated bath disinfectant was tested, and the test process and results are as follows:

[0136] 1. Equipment

[0137] 1. Sample: Cattle hoof disinfectant (diluted 100 times);

[0138] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0139] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0140] 4. Organic interferences: 3% bovine serum albumin;

[0141] 5. Constant temperature incubator (model: 404L);

[0142] 6. Culture medium: ordinary nutrient agar;

[0143] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0144] 8. Standard hard water: hardness 342 mg / L.

[0145] 2. Methods

[0146] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0147] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0148] Cattle hoof disinfectant concentration: 1:100 Action time: 2min;

[0149] The test temperature was 20°C and the experiment was repeated 3 times.

[0150] 3. Test results

[0151] The test results are shown in Table 9

[0152] Table 9 Cattle Hoof Bath Disinfectant Test Results

[0153]

[0154] The rest is the same as in Example 5, except that no surfactant is added, and the other components and processes remain unchanged. The test results of the prepared cattle hoof medicated bath disinfectant are shown in Table 10.

[0155] Table 10 Cattle hoof disinfectant bath test results

[0156]

[0157] Example 6

[0158] S01: Add 6.5g of zinc pyrrolidone carboxylate to 300ml of 5000ppm micro-nano zinc, heat to 55℃ in a beaker and stir for 1.5h, add 1.3g of bovine collagen peptide powder, continue stirring for 1.5h, cool and filter to obtain a precursor solution;

[0159] S02: Take 250 ml of S01 precursor liquid, add 40 g of a mixture of sodium iodide and potassium iodate (1:1), add 35 g of glycerol, add 1.5 g of betaine, add 1.5 g of polyoxyethylene stearyl ether, add 3.5 g of sodium dodecyl sulfate, add 2.6 g of sodium hexadecyl sulfate, add 1.2 g of sodium octadecyl sulfate, add 1500 ml of deionized water, stir at 70°C for 3.0 h, and then cool to obtain cattle hoof medicated bath disinfectant.

[0160] The preparation method of micro-nano zinc is the same as patent 2021107358455.

[0161] The prepared cattle hoof medicated bath disinfectant was tested, and the test process and results are as follows:

[0162] 1. Equipment

[0163] 1. Sample: Cattle hoof disinfectant (diluted 120 times);

[0164] 2. Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0165] 3. Neutralizer: 1% lecithin, 1% Tween-80TSB;

[0166] 4. Organic interferences: 3% bovine serum albumin;

[0167] 5. Constant temperature incubator (model: 404L);

[0168] 6. Culture medium: ordinary nutrient agar;

[0169] 7. Adjustable constant temperature water bath (model: SD28R-30-A12Y);

[0170] 8. Standard hard water: hardness 342 mg / L.

[0171] 2. Methods

[0172] 1. Inspection basis: Technical Specifications for Disinfection, 2002 edition, 2.1.1.5.5, 2.1.1.7.4;

[0173] 2. Neutralizer identification test: Test bacteria: Escherichia coli, Staphylococcus aureus, Candida albicans;

[0174] Concentration of cattle hoof disinfectant bath: 1:120 Action time: 2min;

[0175] The test temperature was 20°C and the experiment was repeated 3 times.

[0176] 3. Test results

[0177] The test results are shown in Table 11

[0178] Table 11 Cattle hoof disinfectant bath test results

[0179]

[0180] The rest is the same as Example 6, except that zinc pyrrolidone carboxylate and surfactant are not added, and other components and processes remain unchanged. The test results of the prepared cattle hoof medicated bath disinfectant are shown in Table 12.

[0181] Table 12 Cattle hoof disinfectant bath test results

[0182]

[0183] The factory inspection report of the cattle hoof medicated bath disinfectant prepared in Example 5 is shown in Table 13.

[0184] Table 13 Factory inspection of cattle hoof disinfectant bath

[0185]

[0186] In order to verify the preventive effect of using cattle hoof disinfectant for hoof warts, interdigital dermatitis and hoof rot, we conducted a 20-day hoof bath verification test at a group ranch in Hebei.

[0187] Experimental method: 1. According to the cattle barn grouping on the ranch, select 2 cattle sheds with good health and divide them into the experimental group and the control group.

[0188] 2. The control group was a blank group, with 90 cows, and no cattle hoof disinfectant was used;

[0189] 3. The 90 cattle in the experimental group were given a hoof bath with a 1:60 dilution of cattle hoof disinfectant. The hoof bath was performed every two days, with the interval between each two hoof baths being the same.

[0190] Test results: The cattle hoof disinfectant has a very good preventive effect on hoof warts and interdigital dermatitis, with a prevention rate of 100%, and a prevention rate of 95.65% for hoof rot. See Table 14 for specific results.

[0191] Table 14 Results of the test on the prevention of hoof warts, interdigital dermatitis and hoof rot by medicated dip disinfectant for cattle hoof

[0192]

[0193] It should be noted that the above content only illustrates the technical idea of ​​the present invention and cannot be used to limit the protection scope of the present invention. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications all fall within the protection scope of the claims of the present invention.

Claims

1. A method for preparing a special cattle hoof medicated bath disinfectant for dairy farms, characterized in that: The following steps are involved: Add zinc pyrrolidone carboxylate to the S01 micro-nano zinc solution, heat to 40°C-60°C, stir for 0.5-2.5h, add bovine collagen peptide powder, continue stirring for 0.5-1.5h, cool and filter to obtain a precursor solution; A mixture of sodium iodide and potassium iodate is added to the S02 precursor solution, and glycerol, a surfactant, and deionized water are added. The mixture is stirred at 60-80°C for 2.5-4.5 hours and then cooled to obtain a cattle hoof medicated bath disinfectant.

2. The method for preparing a special cattle hoof medicated bath disinfectant for dairy farms according to claim 1, characterized in that: step In S01, the mass ratio of micro-nano zinc to zinc pyrrolidone carboxylate is 1:2-5.

3. The method for preparing a special cattle hoof medicated bath disinfectant for dairy farms according to claim 1, characterized in that: step In S01, the mass ratio of micro-nano zinc to bovine collagen peptide powder is 1:0.5-2.

4. The method for preparing a special cattle hoof medicated bath disinfectant for dairy farms according to claim 1, characterized in that: step In S02, the mass ratio of sodium iodide to potassium iodide is 1:

1.

5. The method for preparing a special cattle hoof medicated bath disinfectant for dairy farms according to claim 1, characterized in that: step In S02, the mass ratio of the precursor solution to sodium iodide is 1:0.01-0.

1.

6. The method for preparing a special cattle hoof medicated bath disinfectant for dairy farms according to claim 1, characterized in that: step In S02, the mass ratio of sodium iodide, glycerol, surfactant and deionized water is 1:0.5-3:0.3-5:20-100.

7. The method for preparing a special cattle hoof medicated bath disinfectant for dairy farms according to claim 6, characterized in that: The surfactant is one or more of betaine, polyoxyethylene stearyl alcohol ether, sodium lauryl sulfate, sodium hexadecyl sulfate, and sodium octadecyl sulfate.

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