Amino acid water-soluble fertilizer taking ampullaria gigas as raw material and preparation method of amino acid water-soluble fertilizer

Abstract

The invention relates to the technical field of agricultural fertilizers, and discloses an amino acid water-soluble fertilizer taking ampullaria gigas as a raw material and a preparation method of the amino acid water-soluble fertilizer. The water-soluble fertilizer comprises the following components in percentage by weight: 10%-20% of pomacea canaliculata protein, 0.5%-1.5% of a chelating agent, 2%-5% of carbohydrates, 1%-5% of organic acid, 0.1%-0.3% of a fermentation strain, 1%-2% of water-soluble calcium, 0.1%-0.5% of water-soluble magnesium, 0.2%-0.6% of water-soluble phosphorus, 0.05%-0.1% of a plant growth regulator, 0.05%-0.2% of chelated trace elements and the balance of water, the preparation method comprises the following steps: decomposing pomacea canaliculata protein through a fermentation strain to generate amino acid, reacting with a chelating agent and trace elements, and adding water-soluble calcium, magnesium, phosphorus and other elements to prepare the water-soluble fertilizer. The water-soluble fertilizer can promote rapid growth of crops and improve the yield and quality of the crops, is suitable for various crops, and has the advantages of being environmentally friendly, efficient, easy to absorb and the like.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural fertilizers, in particular to an amino acid water-soluble fertilizer using Pomacea canaliculata as a raw material and a preparation method thereof. Background Art

[0002] With the development of modern agriculture, the demand for efficient and environmentally friendly fertilizers is increasing. Amino acid water-soluble fertilizers have gradually become an important type of fertilizer in agricultural production because they can provide a variety of amino acids and trace elements required by plants and promote the rapid absorption and utilization of crops. The raw materials for the production of traditional amino acid water-soluble fertilizers are mainly derived from animal and plant waste, such as fish meal, plant protein or industrial by-products. These raw materials have certain limitations, such as unstable resource acquisition, large fluctuations in raw material prices, and complex production processes, resulting in high fertilizer production costs. In addition, existing fertilizers usually contain synthetic chemical components, which are prone to adverse effects on the soil and the environment during long-term use, leading to problems such as decreased soil fertility and water pollution.

[0003] As an invasive species, the golden apple snail reproduces rapidly, harming crops such as rice, causing ecological imbalance and agricultural losses. The current treatment of golden apple snails mainly focuses on physical capture and chemical killing, which is time-consuming and labor-intensive and may cause secondary pollution to the environment. The golden apple snail is rich in protein and has the potential to become a source of high-quality amino acids, but there is still a lack of an economical and feasible solution to how to convert this waste resource into an effective agricultural fertilizer. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides an amino acid water-soluble fertilizer using Pomacea canaliculata as a raw material and a preparation method thereof, and utilizes the protein resources of Pomacea canaliculata to prepare a high-efficiency and environment-friendly water-soluble fertilizer rich in amino acids and trace elements through biological fermentation and chelation reaction.

[0005] To achieve the above purpose, the present invention is implemented by the following technical scheme: an amino acid water-soluble fertilizer using Pomacea canaliculata as raw material, comprising the following components by weight percentage:

[0006] Apple snail protein: 10%-20%;

[0007] Chelating agent: 0.5%-1.5%;

[0008] Sugar substances: 2%-5%;

[0009] Organic acid: 1%-5%;

[0010] Fermentation bacteria: 0.1%-0.3%;

[0011] Water-soluble calcium: 1%-2%;

[0012] Water-soluble magnesium: 0.1%-0.5%;

[0013] Water-soluble phosphorus: 0.2%-0.6%;

[0014] Plant growth regulators: 0.05%-0.1%;

[0015] Chelated trace elements: 0.05%-0.2%;

[0016] The balance is water.

[0017] Preferably, the Pomacea canaliculata protein is obtained by biological fermentation and decomposition, and the fermentation bacteria are Bacillus subtilis and / or Bacillus licheniformis.

[0018] Preferably, the fermentation process is carried out at 30-35°C and the fermentation time is 5-7 days.

[0019] Preferably, the chelating agent is EDTA, citric acid or salts thereof, which form stable chelates with trace elements through chelation.

[0020] Preferably, the organic acid is one or more of citric acid, lactic acid and acetic acid.

[0021] Preferably, the water-soluble calcium is generated by the reaction of calcium carbonate and citric acid, and the calcium carbonate is derived from the shell of Pomacea canaliculata.

[0022] The present invention also provides a method for preparing an amino acid water-soluble fertilizer using Pomacea canaliculata as a raw material, comprising the following steps:

[0023] S1. Select uncontaminated golden apple snails, remove the shells and internal organs, and make minced meat from the snail meat;

[0024] S2, mixing the minced Pomacea canaliculata meat with sugar and water, adding fermentation bacteria, and fermenting at 30-35° C. for 5-7 days to obtain a fermentation liquid;

[0025] S3, adding a chelating agent to the fermentation liquid to carry out a chelating reaction with the trace elements;

[0026] S4, adding water-soluble calcium, magnesium and phosphorus to adjust the fertilizer composition;

[0027] S5. Evenly mix the obtained amino acid solution with other ingredients, filter and package.

[0028] Preferably, step S1 comprises the following steps:

[0029] S11, washing the golden apple snails under running water to remove mud and surface impurities;

[0030] S12, soaking the golden apple snails in a 0.5%-1% NaOH alkaline solution for 5-10 minutes to further remove harmful substances on the surface;

[0031] S13, using mechanical or manual methods to remove the shell of the golden apple snail, and retaining the shell for subsequent calcium source treatment;

[0032] S14. Manually or mechanically separate the internal organs of the golden apple snail, especially remove the digestive glands and retain the snail meat;

[0033] S15, mechanically crushing the snail meat to form a meat paste with a particle size of 1-3 mm;

[0034] S16, mixing the minced snail meat with 0.5%-1% citric acid solution to prevent the snail meat from being oxidized, and adjusting the pH to 6-7 for subsequent fermentation.

[0035] Preferably, step S2 comprises the following steps:

[0036] S21, mixing the minced Pomacea canaliculata meat with water, and adding sugar;

[0037] S22, adding Bacillus subtilis and / or Bacillus licheniformis to the mixture of Pomacea canaliculata minced meat and sugar;

[0038] S23, fermenting at a constant temperature of 30-35° C. for 5-7 days, maintaining ventilation and stirring during the fermentation process, stirring 1-2 times a day to ensure uniform fermentation;

[0039] S24, during the fermentation process, the pH value is maintained between 6 and 7, and the pH is adjusted by adding citric acid or sodium carbonate to ensure the activity of the fermentation strain;

[0040] S25, taking samples regularly during fermentation, and terminating the fermentation when the amino acid concentration reaches the target concentration;

[0041] S26. After the fermentation is completed, the fermentation liquid is separated from the solid by a centrifuge to obtain a fermentation liquid rich in amino acids.

[0042] Preferably, the bacterial strains are mixed strains of Bacillus subtilis and Bacillus licheniformis, and the mixing ratio thereof is 1:1.

[0043] The present invention provides an amino acid water-soluble fertilizer using Pomacea canaliculata as a raw material and a preparation method thereof. It has the following beneficial effects:

[0044] 1. The present invention uses the golden apple snail as raw material, fully utilizes its rich protein resources, and generates amino acids through fermentation and decomposition, which not only solves the ecological hazard problem of the golden apple snail, but also converts it into valuable agricultural fertilizer, realizes the recycling of waste resources, and conforms to the concept of environmental protection and sustainable development.

[0045] 2. The present invention produces a variety of amino acids through fermentation and decomposition of Pomacea canaliculata protein, which can be quickly absorbed by plants and significantly improve the nutritional supply of crops. At the same time, through chelation, trace elements such as iron, zinc, manganese, etc. are more easily absorbed and utilized by crops, thereby improving the yield and quality of crops.

[0046] 3. The organic acid and chelating agent contained in the water-soluble fertilizer of the present invention can adjust the soil pH, promote the reproduction of beneficial microorganisms in the soil, and enhance the activity of the soil. Water-soluble calcium, magnesium, phosphorus and other medium elements can effectively improve the soil structure, enhance soil fertility, and ensure the durability of fertilizer effect.

[0047] 4. The plant growth regulators (such as auxin, gibberellin, cytokinin, etc.) added in the present invention can regulate the growth cycle of plants, promote rapid growth and root development of plants, and enhance the stress resistance of crops, including disease resistance, cold resistance, drought resistance, etc., thereby improving the stability of agricultural production and the disease resistance of crops.

[0048] 5. The amino acid water-soluble fertilizer of the present invention is suitable for a variety of crops, including field crops, fruit trees, vegetables and cash crops, and can significantly improve the growth rate and quality of crops and increase yield. At the same time, its water-soluble properties make the fertilizer easier to be absorbed and utilized by crops, and the fertilizer effect is significant, which meets the needs of modern agriculture. DETAILED DESCRIPTION

[0049] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0050] The invention provides an amino acid water-soluble fertilizer using Pomacea canaliculata as a raw material and a preparation method thereof. The water-soluble fertilizer rich in amino acids and trace elements is prepared through process steps such as fermentation of Pomacea canaliculata protein and chelation reaction. The water-soluble fertilizer is suitable for various crops and has the advantages of enhancing crop nutrient absorption, improving stress resistance, and the like.

[0051] The amino acid water-soluble fertilizer using Pomacea canaliculata as raw material in the present invention comprises the following components (by weight percentage):

[0052] Pomacea canaliculata protein (10%-20%): provides the main source of amino acids, can effectively promote crop growth and improve the efficiency of plant nitrogen absorption.

[0053] Chelating agents (0.5%-1.5%): such as EDTA or citric acid, are used to chelate trace elements, increase the absorption and utilization rate of elements such as iron, zinc, and manganese by crops, and prevent the loss of trace elements.

[0054] Sugar substances (2%-5%): such as glucose or sucrose, provide carbon sources for microorganisms during the fermentation process and promote the growth and reproduction of bacteria.

[0055] Organic acid (1%-5%): such as citric acid, lactic acid or acetic acid, adjusts the pH value of the fermentation system, maintains the acid-base balance during the fermentation process, and promotes the activity of fermentation bacteria.

[0056] Fermentation bacteria (0.1%-0.3%): such as Bacillus subtilis and Bacillus licheniformis, are used to decompose the protein of the golden apple snail and produce amino acids and other nutrients.

[0057] Water-soluble calcium (1%-2%): Calcium citrate generated through chelation reaction is an important secondary element in crop growth and enhances cell wall strength.

[0058] Water-soluble magnesium (0.1%-0.5%): Derived from magnesium salts, such as magnesium sulfate, it participates in photosynthesis and improves the utilization of nutrients by crops.

[0059] Water-soluble phosphorus (0.2%-0.6%): provides phosphorus required for plant growth and promotes root development.

[0060] Plant growth regulators (0.05%-0.1%): such as auxin, gibberellin or cytokinin, which promote crop growth and development and regulate plant growth cycle.

[0061] Chelated trace elements (0.05%-0.2%): such as iron, zinc, manganese, etc., enhance the disease resistance and stress resistance of crops.

[0062] Water: The balance, acts as a solvent to ensure that all ingredients are evenly dispersed.

[0063] 2. Preparation method of Pomacea canaliculata amino acid water-soluble fertilizer

[0064] Step S1: Processing of Pomacea canaliculata

[0065] Select uncontaminated golden apple snails and carry out the following processing steps:

[0066] S11. Cleaning: Clean the golden apple snails under running water to remove mud and surface impurities to ensure that the raw materials are clean.

[0067] S12. Alkali treatment: soak the golden apple snails in a 0.5%-1% NaOH alkaline solution for 5-10 minutes to further remove harmful substances on the surface. Rinse with clean water immediately after the alkaline treatment.

[0068] S13. Shelling: Use mechanical or manual methods to remove the shell of the golden apple snail, and keep the shell for subsequent calcium source treatment.

[0069] S14. De-visceration: Manually or mechanically separate the internal organs of the golden apple snail, especially remove the digestive glands, and retain the snail meat to ensure that there are no harmful substances in the fertilizer that are detrimental to crops.

[0070] S15. Crushing: Mechanically crush the snail meat to form a meat paste with a particle size controlled at 1-3 mm to increase the surface area and promote the release of nutrients in the subsequent fermentation process.

[0071] S16, pretreatment: mix the minced snail meat with 0.5%-1% citric acid solution to prevent the snail meat from oxidation and deterioration, and adjust the pH to 6-7, which is suitable for the growth and reproduction of fermentation bacteria.

[0072] Step S2: Fermentation

[0073] The processed Pomacea canaliculata minced meat is mixed with sugar substances and fermented to produce amino acids:

[0074] S21. Prepare culture medium: mix minced Pomacea canaliculata meat and water in a weight ratio of 1:1, and add 2%-5% of sugar substances, such as glucose or sucrose, to provide a carbon source and promote fermentation.

[0075] S22. Inoculating bacteria: adding 0.1%-0.3% of fermentation bacteria, such as Bacillus subtilis and / or Bacillus licheniformis, to the mixture of minced Pomacea canaliculata meat and sugar to promote the decomposition of protein into amino acids.

[0076] S23. Fermentation conditions: Ferment at a constant temperature of 30-35°C for 5-7 days. Keep ventilation and stirring during the fermentation process. Stir 1-2 times a day to ensure uniform fermentation.

[0077] S24. pH control: During the fermentation process, the pH value is maintained between 6 and 7 by adding citric acid or sodium carbonate to ensure the activity of the fermentation bacteria and promote protein degradation.

[0078] S25. Amino acid detection: Samples were taken regularly during the fermentation period, and the amino acid concentration in the fermentation broth was detected by the ninhydrin method. The fermentation was terminated when the amino acid concentration reached the target concentration.

[0079] S26. Fermentation broth separation: After the fermentation is completed, the fermentation broth and solid residue are separated by centrifugation to obtain a fermentation broth rich in amino acids.

[0080] Step S3: Chelation reaction

[0081] Add 0.5%-1.5% of chelating agent, such as EDTA or citric acid, to the fermentation broth to react with trace elements to form stable chelates, thereby improving the absorption efficiency of trace elements. The key elements in the chelation reaction include iron, zinc and manganese.

[0082] Step S4: Addition of calcium, magnesium and phosphorus

[0083] Calcium addition: Calcium carbonate in the snail shell reacts with citric acid to produce calcium citrate (1%-2%), providing water-soluble calcium.

[0084] Magnesium addition: Add 0.1%-0.5% water-soluble magnesium, such as magnesium sulfate, to enhance crop photosynthesis.

[0085] Phosphorus addition: Add 0.2%-0.6% water-soluble phosphorus to promote root development and nutrient absorption of crops.

[0086] Step S5: Mixing and packaging

[0087] The amino acid solution that has undergone chelation and mineral addition is evenly mixed with other ingredients, and high shear mixing equipment is used to ensure that the ingredients are evenly dispersed. After mixing, the remaining particulate impurities are removed by filtration to ensure that the fertilizer is clear. Finally, the finished amino acid water-soluble fertilizer is packaged in plastic barrels and sealed for storage.

[0088] The present invention prepares a highly efficient and environmentally friendly amino acid water-soluble fertilizer by fermenting and decomposing the protein of Pomacea canaliculata, combining chelated trace elements and water-soluble nutrients such as calcium, magnesium, phosphorus, etc. The fertilizer can be quickly absorbed by crops, promote crop growth, increase yield, and enhance crop disease resistance and stress resistance, and is suitable for various agricultural production such as field crops, fruit trees, and vegetables.

[0089] Example 1: Preparation of basic Pomacea canaliculata amino acid water-soluble fertilizer

[0090] formula:

[0091] Apple snail protein: 15%

[0092] Chelating agent (EDTA): 1%

[0093] Sugar (glucose): 3%

[0094] Organic acid (citric acid): 3%

[0095] Fermentation bacteria (Bacillus subtilis): 0.2%

[0096] Water-soluble calcium: 1.5%

[0097] Water-soluble magnesium: 0.3%

[0098] Water-soluble phosphorus: 0.4%

[0099] Plant growth regulator (gibberellic acid): 0.08%

[0100] Chelated trace elements (Fe, Zn, Mn): 0.1%

[0101] Water: Balance to 100%

[0102] Preparation method:

[0103] 1. Select uncontaminated golden apple snails, remove the shells and internal organs, and break the snail meat into 1-3 mm minced meat.

[0104] 2. Mix the minced Pomacea canaliculata meat with water, add 3% glucose and 0.2% Bacillus subtilis, and ferment at 30°C for 6 days. Stir twice a day to keep the fermentation uniform.

[0105] 3. After fermentation is completed, add 1% EDTA chelating agent to carry out chelation reaction with 0.1% of trace elements such as iron, zinc, and manganese.

[0106] 4. Add 1.5% water-soluble calcium, 0.3% water-soluble magnesium and 0.4% water-soluble phosphorus to the fermentation broth.

[0107] 5. After mixing evenly, filter and package into amino acid water-soluble fertilizer.

[0108] The water-soluble fertilizer of this embodiment has a good nutritional supplement effect on vegetable crops, and promotes the development of root systems and rapid growth.

[0109] Example 2: Amino Acid Water-Soluble Fertilizer for Improving Magnesium Content

[0110] formula:

[0111] Apple snail protein: 12%

[0112] Chelating agent (citric acid): 1.2%

[0113] Sugar (sucrose): 4%

[0114] Organic acid (lactic acid): 2%

[0115] Fermentation bacteria (Bacillus licheniformis): 0.25%

[0116] Water-soluble calcium: 1.2%

[0117] Water-soluble magnesium: 0.5%

[0118] Water-soluble phosphorus: 0.5%

[0119] Plant growth regulator (auxin): 0.07%

[0120] Chelated trace elements (Fe, Zn): 0.12%

[0121] Water: Balance to 100%

[0122] Preparation method:

[0123] 1. Mix the minced Pomacea canaliculata meat with sucrose and water, add 0.25% of Bacillus licheniformis fermentation bacteria, and ferment at 32° C. for 7 days.

[0124] 2. During the fermentation process, the pH value is maintained between 6.5-7 and adjusted by adding citric acid and lactic acid.

[0125] 3. After fermentation is complete, add citric acid as a chelating agent to chelate trace elements Fe and Zn.

[0126] 4. Add 1.2% water-soluble calcium, 0.5% water-soluble magnesium and 0.5% water-soluble phosphorus.

[0127] 5. After stirring evenly, filter and package.

[0128] The water-soluble fertilizer in this embodiment is suitable for leafy vegetables, increases the magnesium content, and helps to improve the production of chlorophyll and the efficiency of photosynthesis.

[0129] Example 3: Low-sugar fermented Pomacea canaliculata amino acid water-soluble fertilizer

[0130] formula:

[0131] Apple snail protein: 20%

[0132] Chelating agent (EDTA): 0.7%

[0133] Sugar (glucose): 2%

[0134] Organic acid (acetic acid): 3%

[0135] Fermentation bacteria (Bacillus subtilis): 0.3%

[0136] Water-soluble calcium: 1%

[0137] Water-soluble magnesium: 0.4%

[0138] Water-soluble phosphorus: 0.3%

[0139] Plant growth regulator (gibberellic acid): 0.05%

[0140] Chelated trace elements (Mn, Zn): 0.08%

[0141] Water: Balance to 100%

[0142] Preparation method:

[0143] 1. Process the Pomacea canaliculata to obtain a minced meat material, and mix it with water and 2% glucose.

[0144] 2. Inoculate 0.3% Bacillus subtilis and ferment at a constant temperature of 30°C for 5 days, maintaining ventilation during the fermentation process.

[0145] 3. The fermentation broth reacts with EDTA chelating agent and trace elements (Mn, Zn) to perform chelation.

[0146] 4. Add 1% water-soluble calcium, 0.4% water-soluble magnesium and 0.3% water-soluble phosphorus.

[0147] 5. Filter the finished product and put it into a 10L plastic barrel and seal it.

[0148] This embodiment is a low-sugar fermentation system, which is suitable for fertilizing fruit trees and can improve the disease resistance and fruit quality of fruit trees.

[0149] Example 4: High calcium formula Pomacea canaliculata amino acid water-soluble fertilizer

[0150] formula:

[0151] Apple snail protein: 18%

[0152] Chelating agent (citric acid): 1.5%

[0153] Sugar (glucose): 5%

[0154] Organic acid (lactic acid): 1%

[0155] Fermentation bacteria (Bacillus subtilis): 0.2%

[0156] Water-soluble calcium: 2%

[0157] Water-soluble magnesium: 0.1%

[0158] Water-soluble phosphorus: 0.6%

[0159] Plant growth regulator (cytokinin): 0.1%

[0160] Chelated trace elements (Fe, Zn, Mn): 0.2%

[0161] Water: Balance to 100%

[0162] Preparation method:

[0163] 1. Prepare minced meat using standard Pomacea canaliculata processing methods and mix with 5% glucose.

[0164] 2. Inoculate with 0.2% Bacillus subtilis and ferment at 33°C for 6 days, stirring twice a day.

[0165] 3. After fermentation, add 1.5% citric acid to chelate trace elements such as Fe, Zn, and Mn.

[0166] 4. Add 2% water-soluble calcium, 0.1% water-soluble magnesium and 0.6% water-soluble phosphorus.

[0167] 5. After filtering, you can get high calcium formula water-soluble fertilizer.

[0168] High calcium formula helps improve crop lodging resistance and is particularly suitable for field crops and fruit crops.

[0169] Example 5: Trace element-enhanced amino acid water-soluble fertilizer for Pomacea canaliculata

[0170] formula:

[0171] Apple snail protein: 10%

[0172] Chelating agent (citric acid): 1%

[0173] Sugar (sucrose): 4%

[0174] Organic acid (acetic acid): 2%

[0175] Fermentation bacteria (Bacillus subtilis and Bacillus licheniformis): 0.3%

[0176] Water-soluble calcium: 1%

[0177] Water-soluble magnesium: 0.5%

[0178] Water-soluble phosphorus: 0.5%

[0179] Plant growth regulator (gibberellic acid): 0.08%

[0180] Chelated trace elements (Fe, Zn, Mn, B, Cu): 0.2%

[0181] Water: Balance to 100%

[0182] Preparation method:

[0183] 1. Mix the processed minced Pomacea canaliculata meat with sucrose and water, and inoculate 0.3% of fermentation bacteria (a 1:1 mixture of Bacillus subtilis and Bacillus licheniformis).

[0184] 2. Ferment at a constant temperature of 32°C for 7 days, maintaining the pH at 6.5, adjusted by acetic acid and sodium carbonate.

[0185] 3. After fermentation is completed, add 1% citric acid to chelate trace elements Fe, Zn, Mn, B, and Cu.

[0186] 4. Add 1% water-soluble calcium, 0.5% water-soluble magnesium and 0.5% water-soluble phosphorus.

[0187] 5. After mixing evenly, filter and package the finished product.

[0188] This formula is particularly suitable for crops with high trace element requirements, such as melons and fruits, and can effectively improve the nutrient absorption capacity of crops.

[0189] Comparative experiment 1:

[0190] In order to verify the effect of the water-soluble fertilizer of Pomacea canaliculata amino acid on promoting crop growth, improving crop nutrient absorption and enhancing stress resistance, a comparative experiment was conducted. The comparative experiment was divided into three groups:

[0191] 1. Experimental group: The amino acid water-soluble fertilizer of Pomacea canaliculata of the present invention was used for fertilization, and the water-soluble fertilizer formula of Example 1 was adopted.

[0192] 2. Comparative Example 1: Use a common chemical compound fertilizer (NPK fertilizer) on the market, the main components of which are nitrogen, phosphorus and potassium in a ratio of 20:20:20.

[0193] 3. Comparative Example 2: Using traditional organic fertilizer, the main ingredients of which are animal manure, plant residues and other natural organic matter, without precise nutrient content control.

[0194] Experimental crops:

[0195] Chinese cabbage, which has a short growth period, was selected as the experimental crop. The experimental period was 45 days to test the crop's growth conditions, yield, nutritional components and disease resistance.

[0196] Experimental design:

[0197] Experimental period: 45 days

[0198] Fertilization method: each group was fertilized at the same amount. The experimental group was fertilized with an equal amount of Pomacea canaliculata amino acid water-soluble fertilizer (according to the formula of Example 1) each time, the comparative example 1 was fertilized with an equal amount of NPK compound fertilizer, and the comparative example 2 was fertilized with an equal amount of organic fertilizer.

[0199] Fertilization frequency: Fertilize once every 10 days, for a total of 4 times.

[0200] Measuring indicators:

[0201] Plant growth height (cm): Measure every 10 days and record the growth of plant height.

[0202] Chlorophyll content (SPAD value): measured by a chlorophyll meter to evaluate the photosynthesis capacity of plants.

[0203] Crop fresh weight (g): The fresh weight of each Chinese cabbage was measured at harvest, reflecting the plant biomass.

[0204] Root development: Root health can be evaluated by observing root length and branch density.

[0205] Disease incidence: Disease incidence was recorded to evaluate disease resistance under different fertilizer treatments.

[0206] The comparison results are shown in the following table:

[0207]

[0208] Result analysis:

[0209] Plant growth height: The growth height of the plants in the experimental group was the highest, reaching 30 cm, which was 7.1% and 25% higher than that of Comparative Example 1 and Comparative Example 2, respectively, indicating that the amino acid water-soluble fertilizer of Pomacea canaliculata of the present invention can more effectively promote the longitudinal growth of the plants.

[0210] Chlorophyll content (SPAD value): The chlorophyll content of the experimental group was 40, which was significantly higher than 35 of comparative example 1 and 30 of comparative example 2, indicating that the Pomacea canaliculata amino acid water-soluble fertilizer can better promote plant photosynthesis and enhance the leaf function of crops.

[0211] Average fresh weight: The average fresh weight of each Chinese cabbage in the experimental group reached 300 grams, which was 11.1% higher than that of comparative example 1 and 25% higher than that of comparative example 2, indicating that the water-soluble fertilizer of the present invention can significantly increase the yield of crops.

[0212] Root development: The root length and branch density of the experimental group were better than those of the other two groups. The average root length of the experimental group was 20 cm, while that of comparison groups 1 and B was 17 cm and 15 cm respectively, indicating that the amino acid water-soluble fertilizer of Pomacea canaliculata has a good promoting effect on root development.

[0213] Disease incidence: The disease incidence of the experimental group was only 5%, 10% in comparative example 1, and 15% in comparative example 2. The experimental results show that the amino acid water-soluble fertilizer of the present invention significantly enhances the disease resistance of crops, which may be related to the comprehensive effects of amino acids and trace elements in the fertilizer.

[0214] Through comparative experiments, the experimental group using the water-soluble fertilizer of the amino acid of Pomacea canaliculata showed significant advantages in crop growth, nutrient absorption and disease resistance. Compared with comparative example 1 (NPK fertilizer), the experimental group showed stronger photosynthesis efficiency and better root development; compared with comparative example 2 (organic fertilizer), the experimental group had significant improvements in plant growth rate, yield and disease resistance. It is proved that the water-soluble fertilizer of the amino acid of Pomacea canaliculata of the present invention has significant advantages in increasing production and efficiency.

[0215] Comparative experiment 2:

[0216] In order to verify the effect of the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention in improving the growth of fruit trees, fruit quality and disease resistance, the following comparative experiment was designed.

[0217] Three treatment groups were set up:

[0218] Experimental group: using the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention, the water-soluble fertilizer formula of Example 4 was adopted.

[0219] Comparative Example 3: conventional chemical fertilizer (NPK 15:15:15 compound fertilizer) was used.

[0220] Comparative Example 4: Using traditional organic fertilizers (compost and decomposed animal manure).

[0221] Experimental crops:

[0222] Three-year-old apple trees were selected and the experimental period was 120 days. The growth of the trees, fruit yield, fruit quality and disease resistance were measured.

[0223] Experimental design:

[0224] Experimental period: 120 days

[0225] Fertilization method: Apply equal amount of fertilizer to each fruit tree. The experimental group applies Pomacea canaliculata amino acid water-soluble fertilizer each time, the comparative example 3 applies equal amount of NPK compound fertilizer, and the comparative example 4 applies equal amount of organic fertilizer.

[0226] Fertilization frequency: Fertilize once every 30 days, for a total of 4 times.

[0227] Measuring indicators:

[0228] Crown expansion rate (cm / month): The growth of crown diameter is measured every month, reflecting the growth rate of fruit trees.

[0229] Fruit size (diameter cm): The diameter of the fruit is measured at harvest to reflect the development of the fruit.

[0230] Fruit weight (g): The average weight of the fruit was measured to assess the degree of fruit development.

[0231] Soluble solids content (°Br ix): measures the sweetness of the fruit and reflects the quality of the fruit.

[0232] Disease incidence: Record the disease incidence and evaluate the disease resistance of fruit trees.

[0233] The experimental results are shown in the following table:

[0234]

[0235] Result analysis:

[0236] Crown expansion speed: The crown expansion speed of the fruit trees in the experimental group was 8.5 cm / month, which was significantly higher than that of Comparative Example 3 (7.0 cm / month) and Comparative Example 4 (6.0 cm / month), indicating that the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention can effectively promote the growth of branches and leaves of fruit trees and increase the crown coverage rate.

[0237] Fruit diameter: The average fruit diameter of the experimental group was 8.2 cm, which was 7.9% and 17.1% higher than that of comparative example 3 and comparative example 4, respectively, indicating that the fertilizer of the present invention has a better promoting effect on fruit development.

[0238] Fruit weight: The average fruit weight of the experimental group was 250 g, which was significantly higher than 230 g of Comparative Example 3 and 210 g of Comparative Example 4, indicating that the fertilizer of the present invention can improve the development of fruits and increase fruit yield.

[0239] Soluble solid content: The soluble solid content (i.e. sweetness) of the fruit in the experimental group was 15.0°Br ix, which was higher than that of Comparative Example 3 (13.0°Br ix) and Comparative Example 4 (12.5°Br ix), indicating that the fruit using the Pomacea canaliculata amino acid water-soluble fertilizer had better quality and higher sweetness.

[0240] Disease incidence: The disease incidence in the experimental group was the lowest, only 3%, while the disease incidences in comparative examples 3 and B were 7% and 10%, respectively, indicating that the Pomacea canaliculata amino acid water-soluble fertilizer can effectively improve the disease resistance of fruit trees.

[0241] Through comparative experiments, the experimental group using the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention showed significant advantages in the growth rate, fruit development, fruit quality and disease resistance of fruit trees. The crown expansion speed of the experimental group was fast, the fruit diameter and weight were significantly higher than those of the control group, and the sweetness of the fruit was also higher, showing the excellent effect of the Pomacea canaliculata amino acid water-soluble fertilizer in promoting the growth of fruit trees and improving the quality of fruit. At the same time, the disease incidence rate of fruit trees in the experimental group was much lower than that of the control group, indicating that the water-soluble fertilizer of the present invention has a significant promoting effect on the disease resistance of fruit trees.

[0242] Comparative experiment 3:

[0243] In order to verify the effect of the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention in increasing rice yield, promoting nutrient absorption and improving disease resistance and lodging resistance, the following comparative experiment was designed.

[0244] Three treatment groups were set up:

[0245] Experimental group: using the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention, the water-soluble fertilizer formula of Example 5 was adopted.

[0246] Comparative Example 5: conventional chemical fertilizer (NPK 16:16:16 compound fertilizer) was used.

[0247] Comparative Example 6: Using traditional organic fertilizers (farmyard manure, such as manure and humus).

[0248] Experimental crops:

[0249] Rice was selected as the experimental crop. The experimental period was 120 days. The growth, yield, rice ear development, disease resistance and lodging resistance of rice were measured.

[0250] Experimental design:

[0251] Experimental period: 120 days

[0252] Fertilization method: an equal amount of fertilizer was applied to each rice field. The experimental group applied Pomacea canaliculata amino acid water-soluble fertilizer each time, the comparative example 5 applied an equal amount of NPK compound fertilizer, and the comparative example 6 applied an equal amount of organic fertilizer.

[0253] Fertilization frequency: Fertilize once every 30 days, for a total of 4 times.

[0254] Measuring indicators:

[0255] Plant height (cm): The height of rice plants was measured every 10 days to reflect the growth status.

[0256] Leaf greenness (SPAD value): measured by a chlorophyll meter to evaluate the photosynthetic capacity of rice.

[0257] Number of effective tillers (tillers per plant): Record the number of effective tillers per rice plant to evaluate its tillering ability.

[0258] Number of rice ears (ears / plant): The number of rice ears per rice plant is measured at harvest, reflecting the yield potential of rice.

[0259] Disease incidence: Record the disease incidence and evaluate the disease resistance of rice.

[0260] Lodging rate: Record the proportion of lodged plants to evaluate the lodging resistance of rice.

[0261] The experimental results are shown in the following table:

[0262]

[0263] Result analysis:

[0264] Plant height: The plant height of the rice in the experimental group was 105 cm, which was significantly higher than 95 cm in comparative example 5 and 90 cm in comparative example 6, indicating that the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention has a promoting effect on rice growth.

[0265] Leaf greenness (SPAD value): The SPAD value of rice leaves in the experimental group was 43, which was significantly higher than that of comparative example 5 (39) and comparative example 6 (35), indicating that the use of the fertilizer of the present invention can improve the photosynthesis efficiency of rice and increase the chlorophyll content.

[0266] Number of effective tillers: The number of effective tillers per rice plant in the experimental group was 20, which was significantly higher than 17 in Comparative Example 5 and 15 in Comparative Example 6, indicating that the fertilizer of the present invention can effectively promote rice tillering and increase yield potential.

[0267] Number of rice ears: The average number of rice ears in the experimental group was 15 ears / plant, which was 15.4% and 36.4% higher than that of control examples 5 and B, respectively, indicating that the Pomacea canaliculata amino acid water-soluble fertilizer can effectively increase rice yield.

[0268] Disease incidence: The disease incidence of rice in the experimental group was only 3%, significantly lower than that of comparative example 5 (7%) and comparative example 6 (10%), indicating that the Pomacea canaliculata amino acid water-soluble fertilizer can significantly enhance the disease resistance of rice.

[0269] Lodging rate: The lodging rate of rice in the experimental group was only 2%, while that in comparative example 5 was 8% and that in comparative example 6 was 12%, indicating that the Pomacea canaliculata amino acid water-soluble fertilizer can enhance the stalk strength of rice and significantly improve its lodging resistance.

[0270] Through comparative experiments, the experimental group using the Pomacea canaliculata amino acid water-soluble fertilizer of the present invention showed significant advantages in the growth rate, tillering ability, number of rice ears, and disease resistance and lodging resistance of rice. The plant height, tillering number and number of rice ears of the experimental group rice were significantly higher than those of the comparative example, indicating that the fertilizer of the present invention can effectively improve the yield potential of rice. At the same time, the disease incidence and lodging rate of rice in the experimental group were significantly reduced, indicating that the Pomacea canaliculata amino acid water-soluble fertilizer is effective in enhancing the disease resistance and lodging resistance of rice.

[0271] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An amino acid water-soluble fertilizer using Pomacea canaliculata as raw material, characterized in that: In terms of weight percentage, it includes the following components: Apple snail protein: 10%-20%; Chelating agent: 0.5%-1.5%; Sugar substances: 2%-5%; Organic acid: 1%-5%; Fermentation bacteria: 0.1%-0.3%; Water-soluble calcium: 1%-2%; Water-soluble magnesium: 0.1%-0.5%; Water-soluble phosphorus: 0.2%-0.6%; Plant growth regulators: 0.05%-0.1%; Chelated trace elements: 0.05%-0.2%; The balance is water.

2. The amino acid water-soluble fertilizer using Pomacea canaliculata as raw material according to claim 1, characterized in that: The Pomacea canaliculata protein is obtained by biological fermentation and decomposition, and the fermentation bacteria are Bacillus subtilis and / or Bacillus licheniformis.

3. The amino acid water-soluble fertilizer using Pomacea canaliculata as raw material according to claim 2, characterized in that: The fermentation process is carried out at 30-35°C and the fermentation time is 5-7 days.

4. The amino acid water-soluble fertilizer using Pomacea canaliculata as raw material according to claim 1, characterized in that: The chelating agent is EDTA, citric acid or its salts, which forms a stable chelate with trace elements through chelation.

5. The amino acid water-soluble fertilizer using Pomacea canaliculata as raw material according to claim 1, characterized in that: The organic acid is one or more of citric acid, lactic acid and acetic acid.

6. The amino acid water-soluble fertilizer using Pomacea canaliculata as raw material according to claim 1, characterized in that: The water-soluble calcium is generated by the reaction of calcium carbonate and citric acid, and the calcium carbonate is derived from the shell of Pomacea canaliculata.

7. A method for preparing an amino acid water-soluble fertilizer using Pomacea canaliculata as a raw material according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Select uncontaminated golden apple snails, remove the shells and internal organs, and make minced meat from the snail meat; S2, mixing the minced Pomacea canaliculata meat with sugar and water, adding fermentation bacteria, and fermenting at 30-35° C. for 5-7 days to obtain a fermentation liquid; S3, adding a chelating agent to the fermentation liquid to carry out a chelating reaction with the trace elements; S4, adding water-soluble calcium, magnesium and phosphorus to adjust the fertilizer composition; S5. Evenly mix the obtained amino acid solution with other ingredients, filter and package.

8. The preparation method according to claim 7, characterized in that: The step S1 comprises the following steps: S11, washing the golden apple snails under running water to remove mud and surface impurities; S12, soaking the golden apple snails in a 0.5%-1% NaOH alkaline solution for 5-10 minutes to further remove harmful substances on the surface; S13, using mechanical or manual methods to remove the shell of the golden apple snail, and retaining the shell for subsequent calcium source treatment; S14. Manually or mechanically separate the internal organs of the golden apple snail, especially remove the digestive glands and retain the snail meat; S15, mechanically crushing the snail meat to form a meat paste with a particle size of 1-3 mm; S16, mixing the minced snail meat with 0.5%-1% citric acid solution to prevent the snail meat from being oxidized, and adjusting the pH to 6-7 for subsequent fermentation.

9. The preparation method according to claim 7, characterized in that: The step S2 comprises the following steps: S21, mixing the minced Pomacea canaliculata meat with water, and adding sugar; S22, adding Bacillus subtilis and / or Bacillus licheniformis to the mixture of Pomacea canaliculata minced meat and sugar; S23, fermenting at a constant temperature of 30-35° C. for 5-7 days, maintaining ventilation and stirring during the fermentation process, stirring 1-2 times a day to ensure uniform fermentation; S24, during the fermentation process, the pH value is maintained between 6 and 7, and the pH is adjusted by adding citric acid or sodium carbonate to ensure the activity of the fermentation strain; S25, taking samples regularly during fermentation, and terminating the fermentation when the amino acid concentration reaches the target concentration; S26. After the fermentation is completed, the fermentation liquid is separated from the solid by a centrifuge to obtain a fermentation liquid rich in amino acids.

10. The preparation method according to claim 8, characterized in that: The bacterial strains are mixed strains of Bacillus subtilis and Bacillus licheniformis, and the mixing ratio is 1:1.

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