A multi-channel field stimulation electrode array system

Abstract

The present invention relates to the technical fields of medical device technology and biomedical engineering technology, and discloses a multi-channel field stimulation electrode array system. Culture test tubes are uniformly arranged on the culture plate. A stimulation electrode array unit is assembled in the cover plate. A positive electrode unit and a negative electrode unit corresponding exactly to the culture test tubes are fixed at the bottom of the cover plate, and the positive electrode unit, the negative electrode unit and the stimulation electrode array unit are connected. Different stimulation effects are achieved by controlling different channel fields. The culture plate unit has an electrode array with multiple independent channels, and each channel can be independently controlled to realize multi-channel point stimulation, improve throughput, and generate an electrostimulation field inside the culture test tubes without interference and with strong independence. In addition, the intensity of the electrostimulation field inside this group of culture test tubes can be changed, so as to meet the regulation requirements of the device for giving different electrostimulation when culturing different cells or tissues.

Description

Technical Field

[0001] The present invention relates to the technical fields of medical devices and biomedical engineering, and particularly relates to a multi-channel field stimulation electrode array system. Background Art

[0002] Currently, electrostimulation technology has been widely used in preclinical studies for exploring biological mechanisms, studying medicinal mechanisms, drug screening, efficacy evaluation, etc., especially for cardiomyocytes and nerve cells.

[0003] In an extraocular electrode array device for local electrostimulation of the retina with the application number CN201910648942.3, electrostimulation signals with different parameters are transmitted according to actual stimulation requirements, and a large-area electrostimulation is performed in a direct stimulation mode or a local electrostimulation is performed in an interference stimulation mode. It can be used for electrostimulation treatment of local areas of the retina, and at the same time can be used as a stimulation method for a minimally invasive retinal prosthesis to induce artificial visual sensations in a larger visual field.

[0004] However, traditional electrostimulation technology often uses single-channel or a small number of channels, and the stimulation effect is limited. If a high-intensity regional stress stimulation is used, irreversible damage is likely to occur, and at the same time, it is easy to have problems such as difficulty in precisely controlling the stimulation area and inflexible adjustment of stimulation parameters, which limits its application effect in complex scenarios. With the continuous development of medical technology, the requirements for the accuracy and controllability of electrostimulation technology are getting higher and higher.

[0005] Therefore, the present invention solves the above problems through a multi-channel field stimulation electrode array system. Summary of the Invention

[0006] The purpose of the present invention is to provide a multi-channel field stimulation electrode array system to solve the problems raised in the above background art. The multi-channel electrostimulation technology can provide a more precise, more flexible stimulation scheme, improve the accuracy, controllability, diversity, and high throughput of electrostimulation technology, meet the requirements of different experimental conditions. As an innovative electrostimulation electrode, the array electrode has the advantages of multi-channel, high density, programmable, etc., and can achieve more precise electrostimulation control.

[0007] To achieve the above purpose, the present invention provides the following technical solution: A multi-channel field stimulation electrode array system includes a culture plate unit. The culture plate unit includes a culture plate and a cover plate. Culture test tubes are evenly arranged on the culture plate. A stimulation electrode array unit is assembled in the cover plate. A positive electrode unit and a negative electrode unit corresponding to the culture test tubes are fixed at the bottom of the cover plate, and the positive electrode unit, the negative electrode unit, and the stimulation electrode array unit are connected.

[0008] The stimulating electrode array unit includes a positive electrode and a negative electrode fixed on the left side wall of the cover plate. A main positive electrode wire laid inside the cover plate is connected to the positive electrode, and a main negative electrode wire laid inside the cover plate is connected to the negative electrode. Positive electrode branch lines equal in number to the number of rows of culture test tubes are connected to the main positive electrode wire, and negative electrode branch lines equal in number to the number of rows of culture test tubes are connected to the main negative electrode wire. Each group of positive electrode units is connected to the positive electrode branch line through a conductive wire, and each group of negative electrode units is connected to the negative electrode branch line through a conductive wire.

[0009] Preferably, the culture plate unit further includes liquid adding ports opened on the surface of the culture plate and corresponding to the culture test tubes one by one. A power supply connector is fixed in the middle on the left side of the cover plate. The power supply connector is connected to the positive electrode and the negative electrode. Accommodation grooves corresponding to the positive electrode units and the negative electrode units are opened at the bottom of the cover plate.

[0010] Preferably, sealing rings corresponding to the culture test tubes one by one are fixed at the bottom of the cover plate. The positive electrode units and the negative electrode units are assembled inside the sealing rings. The positive electrode units and the negative electrode units have the same composition structure and are arranged oppositely. The sealing rings are matched with the liquid adding ports.

[0011] Preferably, the positive electrode unit includes a first bracket and a first mounting rack fixed on the left side wall inside the sealing ring. A group of insulating cylinders are hinged and assembled on the front and rear sides of the inner side wall of the first bracket through movable shafts. A positive platinum electrode rod is fixedly assembled inside this group of insulating cylinders.

[0012] Further preferably, a first pushing member is fixed on the left side wall inside the first mounting rack, and a first limiting member is fixed on the right side wall inside the first mounting rack. The other sides of the first pushing member and the first limiting member are fixedly installed on the outer side wall of the positive platinum electrode rod.

[0013] Preferably, the negative electrode unit includes a second bracket and a second mounting rack fixed on the right side wall inside the sealing ring. Another group of insulating cylinders are hinged and assembled on the front and rear sides of the inner side wall of the second bracket through movable shafts. A negative platinum electrode rod is fixedly assembled inside this group of insulating cylinders.

[0014] Further preferably, a second pushing member is fixed on the right side wall inside the second mounting rack, and a second limiting member is fixed on the left side wall inside the second mounting rack. The other sides of the second pushing member and the second limiting member are fixedly installed on the outer side wall of the negative platinum electrode rod.

[0015] Preferably, the first pusher, the first stopper, the second pusher, and the second stopper are all pressure-bearing air bags. The first pusher, the first stopper, the second pusher, and the second stopper are respectively connected to an air pump through a first air pipe, a second air pipe, a third air pipe, and a fourth air pipe. A first solenoid valve, a second solenoid valve, a third solenoid valve, and a fourth solenoid valve are respectively fixedly assembled on the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe.

[0016] Preferably, the positive platinum electrode rod is connected to the wire on the positive branch line, and the negative platinum electrode rod is connected to the wire on the negative branch line. The positive platinum electrode rod and the negative platinum electrode rod are of the same length and are both inserted into the culture test tube.

[0017] Preferably, a multi-channel field stimulation electrode array system further includes an incubator. A storage rack for supporting and limiting the culture plate unit is provided on the inner side wall of the incubator, and the number of the storage racks is the same as that of the culture plate units. A power plug matching the power supply connector is fixed on the inner side wall of the incubator.

[0018] Preferably, a door is hingedly assembled on the front side wall of the incubator, and an electric control box is fixed on the top of the incubator. A display screen matching the culture plate unit is installed on the front side wall of the electric control box.

[0019] The technical effects and advantages of the present invention are as follows:

[0020] 1. When the cell solution is introduced into the culture test tube in the present invention, the positive electrode unit and the negative electrode unit are powered on through the stimulation electrode array unit. A group of culture test tubes is an independent electrode channel. Thus, under the driving action of the stimulation electrode array unit, each electrode channel on the culture plate can be independently controlled, preventing interference during the culture of the culture test tubes and affecting the accuracy of cell condition detection. During the culture, the stimulation electrode array unit is controlled to stimulate cells in different regions to different degrees, and the results of the cell or tissue's response to the stimulation are recorded, improving the precision and controllability of the electrostimulation technology.

[0021] 2. The culture plate in the present invention can cooperate with the stimulation electrode array unit on the cover plate to form a stimulation electrode array with a multi-channel field. Different stimulation effects can be achieved by controlling different channel fields. The number of culture test tubes in the culture plate unit can be customized according to the culture needs, which is convenient for horizontal comparison of different cells during culture, improving the detection accuracy of cells under stress stimulation. During the culture work, the culture plate unit has an electrode array with multiple independent channels, and each channel can be independently controlled to achieve multi-channel point stimulation, improving the throughput. An electric stimulation field is generated inside the culture test tube, without interference from each other, and has strong independence. At the same time, the current value generated in the corresponding culture test tube can be changed, and then the intensity of the electric stimulation field inside the group of culture test tubes can be changed, so as to meet the regulation requirements of the device for different cells or tissues to be given different electric stimulations during culture.

[0022] 3. In order to detect the stimulation effect on cells when different electric stimulation fields are generated by the platinum electrodes, during actual use, a control signal can be sent from the stimulation chip in the electric control box to the air pump, and the cooperation of the air pump and the solenoid valve is used to control the positive electrode unit and the negative electrode unit, which is convenient for adjusting the relative position between the positive platinum electrode rod and the negative platinum electrode rod, so as to change the distribution form of the electric stimulation field in the culture test tube and give more electric stimulations to cells or tissues.

[0023] 4. The present invention detects the use state of the platinum electrodes based on the change of the current value in a single circuit of the platinum electrodes, so as to reflect the passivation degree of the positive platinum electrode rod and the negative platinum electrode rod, which is convenient for making real-time adjustment measures, improving the protection of the platinum electrodes, and at the same time improving the detection accuracy during cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0025] Figure 2 is a schematic diagram of the internal structure of the incubator of the present invention;

[0026] Figure 3 is a schematic diagram of the structure of the culture plate unit of the present invention;

[0027] Figure 4 is a schematic diagram of the disassembled structure of the culture plate unit of the present invention;

[0028] Figure 5 is a schematic diagram of the overall structure of the cover plate of the present invention;

[0029] Figure 6 is a schematic diagram of the partial structure of the cover plate of the present invention;

[0030] Figure 7It is a bottom view of the assembly structure of the positive electrode unit and the negative electrode unit of the present invention;

[0031] Figure 8 It is a top view of the assembly structure of the positive electrode unit and the negative electrode unit of the present invention;

[0032] Figure 9 It is a schematic diagram of the structural layout of the stimulating electrode array unit of the present invention.

[0033] In the figure: 10, incubator; 11, box door; 12, electric control box; 13, display screen; 14, storage rack; 15, power plug; 20, culture plate unit; 21, culture plate; 22, culture test tube; 23, liquid adding port; 24, cover plate; 25, power connector; 26, encapsulation ring; 27, accommodation groove; 30, stimulating electrode array unit; 31, positive electrode; 32, negative electrode; 33, main positive electrode wire; 34, main negative electrode wire; 35, positive electrode branch line; 36, negative electrode branch line; 37, conducting wire; 40, positive electrode unit; 41, first bracket; 42, first mounting bracket; 43, movable shaft; 44, insulating cylinder; 45, positive electrode platinum electrode rod; 46, first pushing member; 47, first limiting member; 50, negative electrode unit; 51, second bracket; 52, second mounting bracket; 53, second pushing member; 54, second limiting member; 55, negative electrode platinum electrode rod. Specific embodiments

[0034] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0035] The first embodiment:

[0036] This embodiment provides a multi-channel field stimulating electrode array system as shown in Figures 1 to 9 , including a culture plate unit 20, which facilitates arranging the number of culture plate units 20 according to actual needs. At the same time, the culture plate unit 20 can be disassembled and replaced, enabling recycling. Setting multiple culture plate units 20 can realize the culture of multiple groups of cells and multiple types of cells, with higher operability.

[0037] Please refer to Figures 2 - 6, The culture plate unit 20 includes a culture plate 21 and a cover plate 24. Culture tubes 22 are uniformly arranged on the culture plate 21. A stimulating electrode array unit 30 is assembled inside the cover plate 24. A positive electrode unit 40 and a negative electrode unit 50 corresponding exactly to the culture tubes 22 are fixed to the bottom of the cover plate 24, and the positive electrode unit 40, the negative electrode unit 50 and the stimulating electrode array unit 30 are connected.

[0038] In this embodiment, the number of groups of the culture tubes 22 designed in the culture plate 21 is 96, and it can be designed as 6, 12, 24, 36, 48, 96, 384 groups according to actual needs. It can cooperate with the stimulating electrode array unit 30 on the cover plate 24 to form a stimulating electrode array with a multi-channel field, and different stimulating effects can be achieved by controlling different channel fields. Then, when cell fluid is introduced into the culture tubes 22, the positive electrode unit 40 and the negative electrode unit 50 are powered on by the stimulating electrode array unit 30. One group of culture tubes 22 is an independent electrode channel. In this way, under the driving action of the stimulating electrode array unit 30, each electrode channel on the culture plate 21 can be independently controlled, preventing interference between the culture tubes 22 during cultivation and affecting the accuracy of cell condition detection. During cultivation, the stimulating electrode array unit 30 is controlled to apply different degrees of stimulation effects to cells in different regions, and the results of the responses of cells or tissues to the stimulation are recorded, improving the precision and controllability of the electrostimulation technology.

[0039] Please refer to Figure 4 and Figure 9 , The stimulating electrode array unit 30 includes a positive electrode 31 and a negative electrode 32 fixed to the left side wall of the cover plate 24. The positive electrode 31 and the negative electrode 32 can ensure the effective operation of the stimulating electrode array unit 30. A main positive line 33 laid inside the cover plate 24 is connected to the positive electrode 31, and a main negative line 34 laid inside the cover plate 24 is connected to the negative electrode 32. The main positive line 33 and the main negative line 34 are laid according to the length of the culture plate 21, which can ensure that each row of culture tubes 22 can be powered on. Positive branch lines 35 with the same number as the number of columns of the culture tubes 22 are connected to the main positive line 33, and negative branch lines 36 with the same number as the number of columns of the culture tubes 22 are connected to the main negative line 34. The positive branch lines 35 and the negative branch lines 36 match the width of the culture plate 21, which can ensure that each column of culture tubes 22 can be powered on. Each group of positive electrode units 40 is connected to the positive branch line 35 through a conducting wire 37, and each group of negative electrode units 50 is connected to the negative branch line 36 through a conducting wire 37. When the positive electrode unit 40 inside the culture tube 22 is powered on, an electric field will be generated inside the culture tube 22 between the positive electrode unit 40 and the negative electrode unit 50, so as to achieve the effect of stimulating the cultured cells or tissues.

[0040] Please refer to Figure 3, the culture plate unit 20 further includes liquid addition ports 23 opened on the surface of the culture plate 21 and corresponding to the culture test tubes 22 one by one. A power connector 25 is fixed in the middle of the left side of the cover plate 24. The power connector 25 is connected to the positive electrode 31 and the negative electrode 32. A receiving groove 27 corresponding to the positive electrode unit 40 and the negative electrode unit 50 is opened at the bottom of the cover plate 24. The culture plate unit 20 is designed to be detachable. At the same time, the power connector 25 adopts a movable plug-in type, which is convenient for operation when replacing the culture plate unit 20. At the same time, the culture plate unit 20 can be quickly powered off without affecting the cell culture of other culture plate units 20.

[0041] Please refer to Figures 4 - 7 , a sealing ring 26 corresponding to the culture test tube 22 one by one is fixed at the bottom of the cover plate 24. The sealing ring 26 is matched with the liquid addition port 23. In this way, when the cover plate 24 is installed on the top of the culture plate 21, the sealing ring 26 can seal the liquid addition port 23. The positive electrode unit 40 and the negative electrode unit 50 are assembled inside the sealing ring 26. The positive electrode unit 40 and the negative electrode unit 50 have the same composition structure and are arranged oppositely, which is convenient for generating an electric field inside the culture test tube 22 by the positive electrode unit 40 and the negative electrode unit 50 after the positive electrode unit 40 is powered on, so as to generate an electric stimulation to the cultured cells or tissues.

[0042] Please refer to Figure 7 and Figure 8 , the positive electrode unit 40 includes a first bracket 41 and a first mounting frame 42 fixed on the inner left side wall of the sealing ring 26. A group of insulating cylinders 44 are hinged and assembled on the front and rear sides of the inner side wall of the first bracket 41 through movable shafts 43. A positive platinum electrode rod 45 is fixedly assembled inside the group of insulating cylinders 44. The negative electrode unit 50 includes a second bracket 51 and a second mounting frame 52 fixed on the inner right side wall of the sealing ring 26. Another group of insulating cylinders 44 are hinged and assembled on the front and rear sides of the inner side wall of the second bracket 51 through movable shafts 43. A negative platinum electrode rod 55 is fixedly assembled inside the group of insulating cylinders 44. The positive platinum electrode rod 45 is connected to the conducting wire 37 on the positive branch line 35, and the negative platinum electrode rod 55 is connected to the conducting wire 37 on the negative branch line 36. The positive platinum electrode rod 45 and the negative platinum electrode rod 55 have the same length and jointly extend into the culture test tube 22.

[0043] During actual use, when the positive electrode 31 is powered on, it can cause the positive platinum electrode rod 45 to be powered on along the main positive electrode line 33, the positive electrode branch line 35, and the conductive line 37. Subsequently, an electric field is generated between the positive platinum electrode rod 45 and the negative platinum electrode rod 55, providing an electric stimulus to the cells in the culture test tube 22. Since all the culture test tubes 22 are independent in the culture plate 21, and after the stimulating electrode array unit 30 is powered on, the voltage in each group of culture test tubes 22 is the same, so that the electric stimulus obtained by each group of culture test tubes 22 is the same, thereby performing an undifferentiated electric stimulus on the cells or tissues on the culture plate unit 20, which is more convenient for recording the cell conditions in each group of culture test tubes 22.

[0044] It should be noted that a multi-channel field stimulation electrode array system disclosed in this embodiment further includes an incubator 10. A storage rack 14 for supporting and limiting the culture plate unit 20 is provided on the inner side wall of the incubator 10, and the number of the storage racks 14 is the same as the number of the culture plate units 20, which is convenient for quickly placing the culture plate unit 20 on the storage rack 14. A power plug 15 matching the power supply connector 25 is fixed on the inner side wall of the incubator 10, which is convenient for the quick insertion of the power plug 15 and the power supply connector 25, and then enables the whole group of culture plate units 20 to be powered on synchronously. A door 11 is hingedly assembled on the front side wall of the incubator 10, and an electric control box 12 is fixed on the top of the incubator 10. A display screen 13 matching the culture plate unit 20 is installed on the front side wall of the electric control box 12. The display screen 13 can display the independent stimulation responses of the culture test tubes 22 inside the culture plate unit 20, and can achieve the independent display of each group of culture test tubes 22, with better visualization effect.

[0045] The electric control box 12 is equipped with a programmable multi-channel micro-current stimulator, including a stimulation chip, a signal transmission module, and a power supply module. Among them: the stimulation chip is connected to the signal transmission module, and the signal transmission module transmits the electric stimulation signal generated after encoding the image information of the external device to the stimulation chip. The stimulation chip is connected to the positive platinum electrode rod 45 and the negative platinum electrode rod 55, and transmits control signals to the positive platinum electrode rod 45 and the negative platinum electrode rod 55 respectively. The power supply module includes a multi-channel voltage source module and a multi-channel voltage amplifier. The multi-channel voltage source module is used to generate a specific frequency and pulse width according to the control signal, and each channel can be set independently. The multi-channel voltage amplifier is used to amplify the voltage signal generated by the voltage source to ensure that the intensity and shape of the voltage meet specific stimulation requirements.

[0046] Second Embodiment:

[0047] This embodiment discloses as Figures 1 to 9A multi-channel field stimulation electrode array system as shown includes all the technical features disclosed in the first embodiment. At the same time, a first pusher 46 is fixed on the left inner wall of the first mounting frame 42, and a first stopper 47 is fixed on the right inner wall of the first mounting frame 42. The other sides of the first pusher 46 and the first stopper 47 are fixedly installed on the outer wall of the positive platinum electrode rod 45. A second pusher 53 is fixed on the right inner wall of the second mounting frame 52, and a second stopper 54 is fixed on the left inner wall of the second mounting frame 52. The other sides of the second pusher 53 and the second stopper 54 are fixedly installed on the outer wall of the negative platinum electrode rod 55.

[0048] During actual use, the positive platinum electrode rod 45 can rotate relative to the movable shaft 43. Similarly, the negative platinum electrode rod 55 can also rotate relative to the movable shaft 43. At the same time, under the clamping action of the first pusher 46 and the first stopper 47, the positive platinum electrode rod 45 can be limited and fixed to prevent the positive platinum electrode rod 45 from self-rotating. Similarly, under the action of the second pusher 53 and the second stopper 54, the negative platinum electrode rod 55 can be limited and fixed to prevent the negative platinum electrode rod 55 from self-rotating, which can ensure the relative fixation of the distance between the positive platinum electrode rod 45 and the negative platinum electrode rod 55, and ensure that the electric stimulation field intensity in each culture test tube 22 is the same.

[0049] It should be noted that the first pusher 46, the first stopper 47, the second pusher 53, and the second stopper 54 are all pressure-bearing air bags, and the first pusher 46, the first stopper 47, the second pusher 53, and the second stopper 54 are respectively connected to an air pump through a first air pipe, a second air pipe, a third air pipe, and a fourth air pipe. First solenoid valves, second solenoid valves, third solenoid valves, and fourth solenoid valves are respectively fixedly assembled on the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe.

[0050] Before the culturing work, the number of culture test tubes 22 can be customized for the culture plate unit 20 according to the culture requirements, which is convenient for horizontal comparison of different cells during culturing and improves the detection accuracy of cells under stress stimulation. During the culturing work, the culture plate unit 20 has an electrode array with multiple independent channels, and each channel can be independently controlled to achieve multi-channel point stimulation, improving the throughput. An electric stimulation field is generated inside the culture test tube 22, without interference from each other and with strong independence.

[0051] It should be noted that due to the need to culture different cells on the same set of culture plates 21, the stimulating electrode array unit 30 is required to have the ability to regulate current, so that different electric stimulation field intensities can be generated in different culture test tubes 22. Therefore, a set of electronically controlled sliding rheostats is connected in series between each positive branch line 35 and the conducting wire 37. In this way, the current value generated in the corresponding culture test tube 22 can be changed by remotely controlling the resistance of the sliding rheostat connected to the circuit, and then the electric stimulation field intensity inside the set of culture test tubes 22 can be changed, so as to meet the device's regulation requirements for different electric stimulations when culturing different cells or tissues.

[0052] The positive platinum electrode rod 45 and the negative platinum electrode rod 55 are made of platinum electrodes, which have the advantages of high conductivity, non-toxicity, low impedance, high stability, and good biocompatibility. Under normal use conditions, the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are both vertically downward and immersed in the cell liquid inside the culture test tube 22, and then an electric stimulation field generated between the positive platinum electrode rod 45 and the negative platinum electrode rod 55 is used to provide stimulation to the cells.

[0053] Moreover, when the platinum electrodes are in use, since the positions of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are relatively fixed, the distribution of the electric stimulation field generated between them is also fixed. In order to detect the stimulating effect of the platinum electrodes on cells when generating different electric stimulation fields, in actual use, a control signal can be sent from the stimulation chip in the electronic control box to the air pump, and the cooperation of the air pump and the solenoid valve is used to control the positive electrode unit 40 and the negative electrode unit 50, so as to conveniently adjust the relative positions of the positive platinum electrode rod 45 and the negative platinum electrode rod 55, and then change the distribution pattern of the electric stimulation field in the culture test tube 22 to give more electric stimulation to the cells or tissues. Specifically:

[0054] When the second solenoid valve and the third solenoid valve are simultaneously controlled to open, at this time, the air pump will introduce gas into the first limiting member 47 and the second pushing member 53 through the second air pipe and the third air pipe respectively, so as to promote the synchronous inflation and expansion of the first limiting member 47 and the second pushing member 53. Then, the first limiting member 47 pushes the insulating cylinder 44 at the top of the positive platinum electrode rod 45 to squeeze the first pushing member 46, so that the positive platinum electrode rod 45 starts to rotate clockwise around the movable shaft 43. Similarly, the second pushing member 53 pushes the insulating cylinder 44 at the top of the negative platinum electrode rod 55 to squeeze the second limiting member 54, so that the negative platinum electrode rod 55 starts to rotate clockwise around the movable shaft 43 synchronously. In this way, the distance between the ends of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 will gradually increase but still remain parallel. At this time, due to the increase in the distance between the ends of the positive platinum electrode rod 45 and the negative platinum electrode rod 55, the intensity of the electric stimulation field between the platinum electrodes will gradually weaken under the condition of constant current, so as to detect the stress response of the cultured cells after the reduction of the electric stimulation field intensity.

[0055] It should be noted that when gas is simultaneously introduced into the first pushing member 46 and the second limiting member 54, the positive platinum electrode rod 45 and the negative platinum electrode rod 55 will rotate counterclockwise around the movable shaft 43 synchronously, and the intensity of the electric stimulation field generated by the platinum electrodes will also be reduced, which is the same as the effect achieved by the above operation.

[0056] When the air pump simultaneously introduces gas into the first pushing member 46 and the second pushing member 53 to make them expand, the first pushing member 46 pushes the positive platinum electrode rod 45 to rotate counterclockwise around the movable shaft 43, while the second pushing member 53 pushes the negative platinum electrode rod 55 to rotate clockwise, so that the distance between the ends of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 is reduced, thereby enhancing the intensity of the electric stimulation field between the platinum electrodes and increasing the stress stimulation on the cultured cells, so as to detect the stress response of the cultured cells after the enhancement of the electric stimulation field intensity.

[0057] However, in actual use, after the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are used for a long time, their surfaces will be oxidized and passivated due to the organic matter in the cell fluid, which will affect the response slope of the platinum electrodes. The most intuitive is that the resistance value of the platinum electrodes becomes larger, and then the current value in the circuit will decrease, and the intensity of the electric stimulation field formed by it will decrease, which makes the response speed of the platinum electrodes significantly slower, resulting in a decrease in the response of the cells in the stress stimulation. The response of the cells in the stress stimulation can detect the magnitude of the current change of the cells through electrophysiological mapping technology or optical mapping technology. Therefore, the degree of passivation of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 can be reflected by detecting the change of the current value in each group of culture test tubes 22, which is convenient to make adjustment measures in real time. Specifically:

[0058] When the decrease change amount of the current in a group of culture test tubes 22 on the display screen 13 exceeds the error threshold, and the power supply voltage of the power plug 15 does not change, it can be determined at this time that the platinum electrodes inside this group of culture test tubes 22 have undergone obvious passivation. At this time, the stimulation chip in the electric control box 12 is used to control the reduction of the resistance value of the sliding rheostat in the electrode array circuit corresponding to this group of culture test tubes 22, so as to increase the current value in this group of electrode array circuits until the current value increases to the same as that in the electrode arrays of other groups, and then increase the electric stimulation field generated by the passivated platinum electrodes.

[0059] When the decrease change amount of the current in a group of culture test tubes 22 on the display screen 13 exceeds the maximum threshold, it indicates at this time that the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are severely passivated to form an oxide film. This not only seriously reduces the intensity of the electric stimulation field generated by the platinum electrodes, but also there will be adsorbents attached to the platinum electrodes, and then the resistance value after the platinum electrodes are connected to the circuit will be greatly increased, seriously affecting the control accuracy of the electric stimulation field during cell culture and also being unfavorable for the detection of cell culture. At this time, the positive platinum electrode rod 45 and the negative platinum electrode rod 55 of this group are taken out for soaking, cleaning, and cleaning.

[0060] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A multi-channel field stimulation electrode array system, characterized in that: The culture plate unit includes a culture plate and a cover plate, culture tubes are evenly arranged on the culture plate, a stimulation electrode array unit is installed in the cover plate, a positive electrode unit and a negative electrode unit corresponding to the culture tubes are fixed at the bottom of the cover plate, and the positive electrode unit, the negative electrode unit and the stimulation electrode array unit are connected; The stimulation electrode array unit includes a positive electrode and a negative electrode fixed on the left side wall of the cover plate, the positive electrode is connected to a main positive electrode line laid inside the cover plate, the negative electrode is connected to a main negative electrode line laid inside the cover plate, the main positive electrode line is connected to positive electrode branches with the same number of culture test tubes, the main negative electrode line is connected to negative electrode branches with the same number of culture test tubes, each group of positive electrode units is connected to the positive electrode branches through a conductive line, and each group of negative electrode units is connected to the negative electrode branches through a conductive line; a group of electrically controlled sliding rheostats is connected in series between each group of positive electrode branches and the conductive line; The positive electrode unit includes a first bracket and a first mounting bracket fixed on the left side wall inside the packaging ring, a group of insulating cylinders are hingedly assembled on the front and rear sides of the inner side wall of the first bracket through a movable shaft, and a positive electrode platinum electrode rod is fixedly assembled inside the group of insulating cylinders; a first pushing member is fixed on the left side wall inside the first mounting bracket, and a first limiting member is fixed on the right side wall inside the first mounting bracket, and the other side of the first pushing member and the first limiting member is fixedly mounted on the outer side wall of the positive electrode platinum electrode rod; The negative electrode unit includes a second bracket and a second mounting bracket fixed on the right side wall inside the packaging ring, and another group of insulating cylinders are hingedly assembled on the front and rear sides of the inner side wall of the second bracket through a movable shaft, and a negative electrode platinum electrode rod is fixedly assembled inside the group of insulating cylinders; a second pushing member is fixed on the right side wall inside the second mounting bracket, and a second limiting member is fixed on the left side wall inside the second mounting bracket, and the other side of the second pushing member and the second limiting member is fixedly mounted on the outer side wall of the negative electrode platinum electrode rod; The first pushing member, the first limiting member, the second pushing member, and the second limiting member are all pressure-bearing air bags, and the first pushing member, the first limiting member, the second pushing member, and the second limiting member are respectively connected to the air pump through the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe, and the first solenoid valve, the second solenoid valve, the third solenoid valve, and the fourth solenoid valve are respectively fixedly assembled on the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe.

2. The multi-channel field stimulation electrode array system according to claim 1, characterized in that: The culture plate unit also includes a liquid filling port opened on the surface of the culture plate and corresponding to the culture test tube. A power connector is fixed in the middle of the left side of the cover plate, and the power connector connects the positive pole and the negative pole. A receiving groove corresponding to the positive electrode unit and the negative electrode unit is opened at the bottom of the cover plate.

3. The multi-channel field stimulation electrode array system according to claim 2, characterized in that: A packaging ring corresponding to the culture tube is fixed at the bottom of the cover plate. The positive electrode unit and the negative electrode unit are assembled inside the packaging ring. The positive electrode unit and the negative electrode unit have the same composition structure and are arranged relatively. The packaging ring matches the liquid filling port.

4. The multi-channel field stimulation electrode array system according to claim 3, characterized in that: The positive platinum electrode rod is connected to the conductive wire on the positive branch line, and the negative platinum electrode rod is connected to the conductive wire on the negative branch line. The positive platinum electrode rod and the negative platinum electrode rod have the same length and extend into the culture tube together.

5. The multi-channel field stimulation electrode array system according to claim 4, characterized in that: It also includes an incubator, on the inner wall of which are arranged racks for supporting and limiting the culture plate units, the number of the racks is the same as the number of the culture plate units, and a power plug matching the power connector is fixed on the inner wall of the incubator.

6. The multi-channel field stimulation electrode array system according to claim 5, characterized in that: A box door is hingedly mounted on the front side wall of the incubator, an electric control box is fixed on the top of the incubator, and a display screen matching the culture plate unit is installed on the front side wall of the electric control box.

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