Method for testing sterilization capability of sterilization space of equipment

Abstract

The invention relates to the technical field of intelligent household appliances, and discloses a method for testing the sterilization capability of a sterilization space of equipment. Respectively preparing the control bacterial liquid group and the sterilization bacterial liquid group into a control test sample group and a sterilization test sample group; obtaining a corresponding sterilization test culture group and a control test culture group; obtaining first bacterial colony information of the sterilization test culture group and second bacterial colony information of the control test culture group; according to the first bacterial colony information and the second bacterial colony information, the sterilization capacity of the sterilization space is obtained. The sterilization test sample group is put into the sterilization space of the equipment, the contrast test sample group is put into the contrast space with the same volume as the sterilization space of the equipment, and the first bacterial colony information and the second bacterial colony information are obtained, so that the sterilization capability of the sterilization space is obtained, and the test and evaluation of the sterilization effect in the sterilization space of the equipment can be realized.

Description

Technical Field

[0001] The present application relates to the field of smart home appliance technology, for example, to a method for testing the sterilization capability of a sterilization space of a device. Background Art

[0002] At present, in recent years, food safety and sterilization functions in refrigerators have become the focus of public attention. Among them, the microorganisms produced by the corruption of food during storage have a polluting effect on the refrigerator environment. Newly placed food is more likely to be corrupted when stored in an environment with microbial contamination, and the microorganisms and odors produced affect the health of consumers, and there are also hidden dangers to food safety. Consumers' demand for food safety sterilization in refrigerators requires the development of sterilization modules equipped in refrigerators and methods that can evaluate the sterilization effect.

[0003] There are existing testing methods for the purification and sterilization rate of air purifiers, but there is currently no testing and evaluation method for the sterilization effect of space in household appliances such as refrigerators.

[0004] It should be noted that the information disclosed in the above background technology section is only used to enhance the understanding of the background of the present application, and therefore may include information that does not constitute the prior art known to ordinary technicians in the field. Summary of the invention

[0005] In order to provide a basic understanding of some aspects of the disclosed embodiments, a brief summary is given below. The summary is not an extensive review, nor is it intended to identify key / critical components or delineate the scope of protection of these embodiments, but rather serves as a prelude to the detailed description that follows.

[0006] The disclosed embodiments provide a method for testing the sterilization capability of a sterilization space of an equipment, so as to solve the technical problem that there is currently no testing and evaluation method for the sterilization effect of the space of household appliances such as refrigerators.

[0007] In some embodiments, a method for testing the sterilization ability of a sterilization space of an equipment is provided, comprising:

[0008] Prepare bacterial solution for testing;

[0009] The test bacterial solution was prepared into a control bacterial solution group and a bactericidal bacterial solution group;

[0010] The control bacterial solution group and the bactericidal bacterial solution group were prepared as a control test sample group and a bactericidal test sample group respectively;

[0011] The sterilization test sample group is placed in the sterilization space of the equipment for sterilization test to obtain the sterilization sample group after the test; during the sterilization test, the equipment is started to operate so that the sterilization space reaches the set test conditions, and the equipment runs for a preset test time;

[0012] Place the control test sample group in a control space with the same volume as the sterilization space of the equipment for control testing to obtain a post-test control sample group; during the control test, make the control space have environmental conditions consistent with the set test conditions of the sterilization test and place it for a preset test time;

[0013] Performing bacterial culture on the post-test sterilization sample group and the post-test control sample group respectively to obtain a sterilization test culture group and a control test culture group;

[0014] Obtaining first colony information of a sterilization test culture group and second colony information of a control test culture group;

[0015] The sterilization capacity of the sterilization space is obtained according to the first colony information and the second colony information.

[0016] In these embodiments, a test and evaluation method for the sterilization effect of the space of household appliances such as refrigerators is proposed. By placing a sterilization test sample group into a sterilization space of the device and placing a control test sample group into a control space with the same volume as the sterilization space of the device, first colony information and second colony information are obtained. Based on the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, thereby realizing the test and evaluation of the sterilization effect in the sterilization space of the device.

[0017] In some embodiments, the preparation of the test bacterial solution comprises:

[0018] The pasteurized milk is subjected to rejuvenation culture to obtain a test bacterial solution with a set colony count; wherein the rejuvenation culture includes shaking culture for a first period of time, or static culture for a second period of time.

[0019] In these embodiments, pasteurized milk is used to prepare the test bacterial solution, and colonies are easy to obtain, thereby reducing the disadvantages of difficult acquisition of professional strains for microbiological operations, high costs, and strict requirements for storage equipment.

[0020] In some embodiments, the control bacterial solution group and the bactericidal bacterial solution group are respectively prepared as a control test sample group and a bactericidal test sample group, including:

[0021] Spread the bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group onto sterilized glass slides or sterilized filter paper sheets respectively, to obtain a first control test sample group and a first sterilization test sample group respectively;

[0022] Then, the first control test sample group is subjected to a sterilization test to obtain a first post-test control sample group, and the first sterilization test sample group is subjected to a control test to obtain a first post-test sterilization sample group. In some embodiments, bacterial culture is performed on the post-test sterilization sample group and the post-test control sample group, respectively, including:

[0023] The first post-test sterilization sample group and the first post-test control sample group are respectively placed in a second volume of physiological saline of a first concentration, and balanced at a second temperature for a third time, thereby obtaining a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group;

[0024] Respectively pipette a first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample, and add them into sterile culture plates;

[0025] A first volume of plate count agar medium is injected into each sterile culture plate, and then the sterile culture plate is placed in an environment of a third temperature and cultured for a fourth time period.

[0026] In these embodiments, a method of separately culturing bacteria in the post-test sterilization sample group and the post-test control sample group is proposed.

[0027] In some embodiments, the control bacterial solution group and the bactericidal bacterial solution group are respectively prepared as a control test sample group and a bactericidal test sample group, including:

[0028] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group are respectively spread on the bottom surface of the sterile culture dish or on the agar solidified layer laid on the bottom surface of the sterile culture dish, to obtain a second control test sample group and a second sterilization test sample group;

[0029] Then, the second control test sample group is subjected to a sterilization test to obtain a second post-test control sample group, and the second sterilization test sample group is subjected to a control test to obtain a second post-test sterilization sample group. In some embodiments, bacterial culture is performed on the post-test sterilization sample group and the post-test control sample group, respectively, including:

[0030] Covering the bacterial nutrient gel suspension or the plate count agar medium on the surfaces of the culture dishes of the second post-test sterilization sample group and the second post-test control sample group, respectively, and placing the bacterial nutrient gel suspension or the plate count agar medium in an environment of a fourth temperature for a sixth period of time after solidification of the bacterial nutrient gel suspension or the plate count agar medium;

[0031] The bacterial nutrient gel suspension includes, by weight percentage, 0.2% to 0.6% soy protein, 0.1% to 0.8% trypsin, 0.05% to 0.45% glucose, 2% to 8% sodium chloride, 0.05% to 0.5% dipotassium hydrogen phosphate, 0.2% to 0.9% agar, and water is supplemented to 100%.

[0032] In these embodiments, a gel suspension medium containing soybean protein, trypsin, glucose, sodium chloride, dipotassium hydrogen phosphate, agar and water is prepared and, after sterilization, is covered on the surface of the plate, thereby reducing the loss effect of the microorganism elution process.

[0033] In these embodiments, agar and plate count agar medium are used as double-layer medium covering and bottom colony coating. The colony distribution is more uniform, the influence of colony elution is reduced, and the feasibility of adjusting different dilution gradients is higher because the colony operation space becomes larger.

[0034] In some embodiments, the first colony information includes a first bacterial content; the second colony information includes a second bacterial content;

[0035] According to the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, including:

[0036] The test sterilization rate is calculated according to the following relationship (1):

[0037] k1=f1×(C1-C2)÷C1 (1)

[0038] Among them, k1 is the test sterilization rate, the unit is %; C1 is the second bacterial content, the unit is cfu / ml or cfu / g; C2 is the first bacterial content, the unit is cfu / ml or cfu / g; f1 is the time coefficient, f1=a / t, t is the preset test time, and a is the first adjustment parameter.

[0039] In some embodiments, the value of a is 1, and in other embodiments, the value of a is 0.5.

[0040] In these embodiments, a method of achieving sterilization capability of a sterilized space is proposed.

[0041] In some embodiments, the test sterilization rate is calculated according to the following relationship (2):

[0042] k1=f1×f2×(C1-C2)÷C1 (2)

[0043] Wherein, f2 is the space coefficient, f2=b÷V; V is the volume of the sterilization space, and b is the second adjustment parameter. In some embodiments, the value of b is 625.

[0044] In these embodiments, a method of achieving sterilization capability of a sterilized space is proposed.

[0045] In some embodiments, the first colony information includes a first colony area parameter; the second colony information includes a second colony area parameter;

[0046] According to the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, including:

[0047] The sterilization parameters are calculated according to the following equation (3):

[0048]

[0049] Among them, k3 is the sterilization parameter; f3 is the evaluation coefficient; S1 is the second colony area parameter, and S2 is the first colony area parameter.

[0050] In these embodiments, a method of achieving sterilization capability of a sterilized space is proposed.

[0051] In some embodiments, the colony area parameter is calculated according to the following relationship (4):

[0052] S=N×A (4)

[0053] Wherein, N is the dilution factor and A is the colony area score.

[0054] The test method for the sterilization ability of the sterilization space of the equipment provided in the embodiment of the present disclosure can achieve the following technical effects:

[0055] A test and evaluation method for the sterilization effect of the space of household appliances such as refrigerators is proposed. The method comprises placing a sterilization test sample group into the sterilization space of the device, placing a control test sample group into a control space with the same volume as the sterilization space of the device, obtaining first colony information and second colony information, and obtaining the sterilization capacity of the sterilization space based on the first colony information and the second colony information, thereby realizing the test and evaluation of the sterilization effect in the sterilization space of the device.

[0056] The above general description and the following description are exemplary and explanatory only and are not intended to limit the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] One or more embodiments are exemplarily described by corresponding drawings, which do not limit the embodiments. Elements with the same reference numerals in the drawings are shown as similar elements, and the drawings do not constitute a scale limitation, and wherein:

[0058] Figure 1 It is a flow chart of a method for testing the sterilization capacity of a sterilization space of an equipment;

[0059] Figure 2 It is a schematic diagram of a process for preparing a bacterial solution for testing provided by an embodiment of the present disclosure;

[0060] Figure 3 is a schematic flow chart of another method for preparing a bacterial solution for testing provided by an embodiment of the present disclosure;

[0061] Figure 4 It is a schematic diagram of a sterilization experiment method according to an embodiment of the present disclosure;

[0062] Figure 5is a schematic diagram of another method flow of a sterilization experiment according to an embodiment of the present disclosure;

[0063] Figure 6 It is a schematic diagram of another method flow of a sterilization experiment provided by an embodiment of the present disclosure;

[0064] Figure 7 It is a schematic diagram of another method flow of a sterilization experiment provided by an embodiment of the present disclosure;

[0065] Figure 8 It is a flow chart of a method for determining a test sterilization rate provided by an embodiment of the present disclosure;

[0066] Fig. 9 It is a flow chart of another method for determining a test sterilization rate provided by an embodiment of the present disclosure;

[0067] Fig.10 This is a method provided by the embodiment of the present disclosure to obtain the sterilization capability of a sterilization space. DETAILED DESCRIPTION

[0068] In order to be able to understand the features and technical contents of the embodiments of the present disclosure in more detail, the implementation of the embodiments of the present disclosure is described in detail below in conjunction with the accompanying drawings. The attached drawings are for reference only and are not used to limit the embodiments of the present disclosure. In the following technical description, for the convenience of explanation, a full understanding of the disclosed embodiments is provided through multiple details. However, one or more embodiments can still be implemented without these details. In other cases, to simplify the drawings, well-known structures and devices can be simplified for display.

[0069] The terms "first", "second", etc. in the specification and claims of the embodiments of the present disclosure and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the terms used in this way can be interchanged where appropriate, so that the embodiments of the embodiments of the present disclosure described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions.

[0070] Unless otherwise stated, the term "plurality" means two or more.

[0071] In the embodiment of the present disclosure, the character " / " indicates that the preceding and following objects are in an "or" relationship. For example, A / B indicates: A or B.

[0072] The term "and / or" is a description of the association relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A or B, or, A and B.

[0073] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other.

[0074] The disclosed embodiments provide a method for testing the sterilization capability of a sterilization space of an equipment, so as to solve the technical problem that there is currently no testing and evaluation method for the sterilization effect of the space of household appliances such as refrigerators.

[0075] In some embodiments, Figure 1 As shown, a test method for the sterilization ability of a sterilization space of an equipment is provided, comprising:

[0076] S101, preparing bacterial solution for testing.

[0077] S102, preparing the test bacterial solution into a control bacterial solution group and a bactericidal bacterial solution group.

[0078] S103, preparing the control bacterial solution group and the bactericidal bacterial solution group into a control test sample group and a bactericidal test sample group respectively.

[0079] S104, placing the sterilization test sample group into the sterilization space of the equipment for sterilization test, and obtaining the sterilization sample group after the test; during the sterilization test, the equipment is started to operate so that the sterilization space reaches the set test conditions, and the equipment runs for a preset test time.

[0080] S105, placing the control test sample group in a control space with the same volume as the sterilization space of the equipment for control testing, and obtaining a post-test control sample group; in the control test, the control space is made to have environmental conditions consistent with the set test conditions of the sterilization test, and is placed for a preset test time. Here, the control space of the control test does not have sterilization capability.

[0081] S106. Performing bacterial culture on the post-test sterilization sample group and the post-test control sample group respectively to obtain a sterilization test culture group and a control test culture group.

[0082] S107, obtaining the first colony information of the sterilization test culture group and the second colony information of the control test culture group.

[0083] S108. Obtain the sterilization capacity of the sterilization space according to the first colony information and the second colony information.

[0084] In these embodiments, a test and evaluation method for the sterilization effect of the space of household appliances such as refrigerators is proposed. By placing a sterilization test sample group into a sterilization space of the device and placing a control test sample group into a control space with the same volume as the sterilization space of the device, first colony information and second colony information are obtained. Based on the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, thereby realizing the test and evaluation of the sterilization effect in the sterilization space of the device.

[0085] In some embodiments, the test bacterial solution is prepared from pasteurized milk.

[0086] In some embodiments, the preparation of the test bacterial solution comprises:

[0087] The pasteurized milk is subjected to rejuvenation culture to obtain a test bacterial solution with a set colony count, wherein the rejuvenation culture includes shaking culture for a first time period, or static culture for a second time period.

[0088] In practical applications, the first duration can be 2 hours to 5 hours, and the second duration can be 12 hours to 24 hours. The set colony count can be 1×10 5 cfu / ml to 1×10 8 cfu / ml.

[0089] In these embodiments, pasteurized milk is used to prepare the test bacterial solution, and colonies are easy to obtain, thereby reducing the disadvantages of difficult acquisition of professional strains for microbiological operations, high costs, and strict requirements for storage equipment.

[0090] In practical applications, multiple concentrations of test bacterial solutions can be used in the experiment to improve the accuracy of the experiment. Multiple concentrations of test bacterial solutions can be prepared by gradient dilution method to obtain a bactericidal bacterial solution group. In some embodiments, two to three concentrations of test bacterial solutions can be prepared. Similarly, a control bacterial solution group is obtained.

[0091] In some embodiments, in combination Figure 2 As shown, preparing the test bacterial solution includes:

[0092] S201, placing the pasteurized milk into a triangular flask of a first volume for colony rejuvenation at a first rotation speed.

[0093] S202, shaking and culturing the pasteurized milk in the conical flask at a first temperature for a first period of time.

[0094] S203, diluting the cultured pasteurized milk according to a gradient dilution method to generate 2 to 3 concentrations of test bacterial solutions.

[0095] In practical applications, the first volume may be 50 ml, the first temperature may be 30° C., and the first rotation speed may be 100 r / min.

[0096] In some embodiments, in combination Figure 3 As shown, prepare the test bacterial solution, including:

[0097] S301, placing the pasteurized milk into a triangular flask of a first volume for colony rejuvenation at a first rotation speed.

[0098] S302, culturing the pasteurized milk in the flask at the first temperature for a second period of time.

[0099] S303, diluting the cultured pasteurized milk according to a gradient dilution method to generate 2 to 3 concentrations of test bacterial solutions.

[0100] In some embodiments, the control bacterial solution group and the bactericidal bacterial solution group are respectively prepared as a control test sample group and a bactericidal test sample group, including:

[0101] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group are respectively spread onto a sterilized glass slide or a sterilized filter paper sheet to obtain a first control test sample group and a first sterilization test sample group accordingly.

[0102] Accordingly, a first post-test sterilization sample group and a first post-test control sample group are obtained.

[0103] In this embodiment, it can be understood that the sterilization sample group after the first test is obtained by placing the first sterilization test sample group into the sterilization space of the device, and the device runs the preset test time under the set test conditions. The control sample group after the first test is obtained by placing the first control test sample group into a control space with the same volume as the sterilization space of the device, and making the control space have environmental conditions consistent with the set test conditions, and placing it for the preset test time.

[0104] In some embodiments, the post-test sterilization sample group and the post-test control sample group are subjected to bacterial culture respectively, comprising:

[0105] The first post-test sterilization sample group and the first post-test control sample group are respectively placed in a second volume of physiological saline of a first concentration, and equilibrated at a second temperature for a third time to obtain a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group.

[0106] A first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample are respectively pipetted and added into sterile culture plates.

[0107] A first volume of plate count agar medium is injected into each sterile culture plate, and then the sterile culture plate is placed in an environment of a third temperature and cultured for a fourth time period.

[0108] In these embodiments, a method of separately culturing bacteria in the post-test sterilization sample group and the post-test control sample group is proposed.

[0109] In practical applications, the second volume can be 100 ml. The first concentration can be 0.75%. The second temperature can be 20° C. to 30° C. The third duration can be 15 min to 30 min. The first volume can be 15 ml to 25 ml. The third temperature can be 30° C. The fourth duration can be 48 h. During the process of balancing the third duration at the second temperature, the balancing can be performed at a stirring speed of 100 r / min to 150 r / min.

[0110] Combination Figure 4 As shown, in some embodiments, the experimental process after preparing the test bacterial solution includes:

[0111] S401 , spreading the bacterial solutions of the control bacterial solution group and the bactericidal bacterial solution group onto sterilized glass slides respectively, to obtain a first control test sample group and a first bactericidal test sample group accordingly.

[0112] S402: Place the first sterilization test sample group into the sterilization space of the device. Under the set test conditions, the device runs the preset test time to obtain the first post-test sterilization sample group.

[0113] S403, placing the first control test sample group in a control space with the same volume as the sterilization space of the equipment, and making the control space have environmental conditions consistent with the set test conditions, and placing it for a preset test time to obtain a first post-test control sample group.

[0114] S404, respectively placing the first post-test sterilization sample group and the first post-test control sample group into a second volume of physiological saline of a first concentration, equilibrating at a second temperature for a third period of time, and correspondingly obtaining a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group.

[0115] S405 , respectively aspirate the first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample, and add them into sterile culture dishes respectively.

[0116] S406, injecting a first volume of plate count agar medium into each sterile culture plate, and then placing the sterile culture plate in an environment at a third temperature and culturing for a fourth time period.

[0117] Combination Figure 5 As shown, in some embodiments, the experimental process after preparing the test bacterial solution includes:

[0118] S501 , spreading the bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group onto sterilized filter paper sheets respectively, to obtain a first control test sample group and a first sterilization test sample group respectively.

[0119] S502: Place the first sterilization test sample group into the sterilization space of the device. Under the set test conditions, the device runs the preset test time to obtain the first post-test sterilization sample group.

[0120] S503, placing the first control test sample group in a control space with the same volume as the sterilization space of the equipment, and making the control space have environmental conditions consistent with the set test conditions, and placing it for a preset test time to obtain a first post-test control sample group.

[0121] S504, respectively placing the first post-test sterilization sample group and the first post-test control sample group into a second volume of physiological saline of a first concentration, and equilibrating them at a second temperature for a third time, to obtain a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group.

[0122] S505 , respectively aspirate the first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample, and add them into sterile culture plates respectively.

[0123] S506, injecting a first volume of plate count agar medium into each sterile culture plate, and then placing the sterile culture plate in an environment at a third temperature and culturing for a fourth time period.

[0124] In some embodiments, the control bacterial solution group and the bactericidal bacterial solution group are respectively prepared as a control test sample group and a bactericidal test sample group, including:

[0125] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group are respectively spread on the bottom surface of the sterile culture dish or on the agar solidified layer laid on the bottom surface of the sterile culture dish, and a second control test sample group and a second sterilization test sample group are obtained accordingly.

[0126] Accordingly, a second post-test sterilization sample group and a second post-test control sample group are obtained.

[0127] In this embodiment, it can be understood that the second post-test sterilization sample group is obtained by placing the second sterilization test sample group into the sterilization space of the device, and the device runs the preset test time under the set test conditions. The second post-test control sample group is obtained by placing the second control test sample group into a control space with the same volume as the sterilization space of the device, and making the control space have environmental conditions consistent with the set test conditions, and placing it for the preset test time.

[0128] In some embodiments, the post-test sterilization sample group and the post-test control sample group are subjected to bacterial culture respectively, comprising:

[0129] The bacterial nutrient gel suspension or the plate count agar medium is respectively covered on the culture dish surfaces of the second post-test sterilization sample group and the second post-test control sample group; after the bacterial nutrient gel suspension or the plate count agar medium solidifies, it is placed in an environment of a fourth temperature and cultured for a sixth time period.

[0130] The bacterial nutrient gel suspension includes, by weight percentage, 0.2% to 0.6% soy protein, 0.1% to 0.8% trypsin, 0.05% to 0.45% glucose, 2% to 8% sodium chloride, 0.05% to 0.5% dipotassium hydrogen phosphate, 0.2% to 0.9% agar, and water is supplemented to 100%.

[0131] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.2% soy protein, 0.1% trypsin, 0.05% glucose, 2% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.2% agar, and water is supplemented to 100%.

[0132] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.6% soy protein, 0.8% trypsin, 0.45% glucose, 8% sodium chloride, 0.5% dipotassium hydrogen phosphate, 0.9% agar, and water is supplemented to 100%.

[0133] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.3% soy protein, 0.7% trypsin, 0.3% glucose, 6% sodium chloride, 0.4% dipotassium hydrogen phosphate, and 0.7% agar, and water is supplemented to 100%.

[0134] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.4% soy protein, 0.7% trypsin, 0.4% glucose, 7% sodium chloride, 0.5% dipotassium hydrogen phosphate, and 0.8% agar, and water is supplemented to 100%.

[0135] In practical applications, the fourth temperature may be 30° C., and the sixth time period may be 48 hours.

[0136] Combination Figure 6 As shown, in some embodiments, the experimental process after preparing the test bacterial solution includes:

[0137] S601, spreading the bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group on the bottom surface of a sterile culture dish, and obtaining a second control test sample group and a second sterilization test sample group accordingly.

[0138] S602: Place the second sterilization test sample group into the sterilization space of the device. Under the set test conditions, the device runs the preset test time to obtain the second post-test sterilization sample group.

[0139] S603, placing the second control test sample group in a control space with the same volume as the sterilization space of the equipment, and making the control space have environmental conditions consistent with the set test conditions, and placing it for a preset test time to obtain a second post-test control sample group.

[0140] S604. Prepare a gel suspension culture medium containing soybean protein, trypsin, glucose, sodium chloride, dipotassium hydrogen phosphate, agar and water.

[0141] S605, respectively covering the surfaces of the culture dishes of the sterilized sample group after the second test and the control sample group after the second test with the bacterial nutrient gel suspension, and placing the culture dishes in an environment of a fourth temperature for a sixth period of time after the bacterial nutrient gel suspension solidifies.

[0142] In these embodiments, a gel suspension medium containing soybean protein, trypsin, glucose, sodium chloride, potassium hydrogen phosphate, agar and water is prepared and coated on the surface of the plate, thereby reducing the loss effect of the microorganism elution process.

[0143] Combination Figure 7 As shown, in some embodiments, the experimental process after preparing the test bacterial solution includes:

[0144] S701, spreading the bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group onto the agar solidified layer laid on the bottom of the sterile culture dish, and obtaining a second control test sample group and a second sterilization test sample group accordingly.

[0145] S702: Place the second sterilization test sample group into the sterilization space of the device. Under the set test conditions, the device runs the preset test time to obtain the second post-test sterilization sample group.

[0146] S703, placing the second control test sample group in a control space with the same volume as the sterilization space of the equipment, and making the control space have environmental conditions consistent with the set test conditions, and placing it for a preset test time to obtain a second post-test control sample group.

[0147] S704, respectively covering the culture dish surfaces of the second post-test sterilization sample group and the second post-test control sample group with plate count agar medium, and placing them in an environment of a fourth temperature for a sixth period of time after the bacterial nutrient gel suspension or the plate count agar medium solidifies.

[0148] In these embodiments, agar and plate count agar medium are used as double-layer medium covering and bottom colony coating. The colony distribution is more uniform, the influence of colony elution is reduced, and since the colony operation space becomes larger, it is more feasible to adjust different dilution gradients.

[0149] In step S701, the agar solidified layer laid on the bottom surface of the sterile culture dish is obtained by the following steps:

[0150] Weigh 7g to 25g of agar, add 1000mL of grade 3 pure water to prepare a 0.7% to 2.5% agar suspension, and dispense into Erlenmeyer flasks. Sterilize at 121℃ for 15min to 20min, select a disposable sterile culture plate, pour 15mL to 20mL of sterilized agar solution, and solidify for 1h to 12h.

[0151] In some embodiments, the first colony information includes a first bacterial content, and the second colony information includes a second bacterial content.

[0152] In some embodiments, a method for testing the sterilization capability of a sterilization space of a device is provided, comprising:

[0153] The processor obtains a first bacterial content of a sterilization test of a sterilization liquid set in a sterilization space of the device, and a second bacterial content of a control liquid in a sterilization test having the same storage environment as the sterilization liquid.

[0154] The processor determines an initial sterilization rate of the sterilization space of the device according to the first bacteria content and the second bacteria content.

[0155] The processor obtains a test environment coefficient of a sterilization space of the equipment.

[0156] The processor corrects the initial sterilization rate using the test environment coefficient to obtain a test sterilization rate of the sterilization space of the equipment, so as to evaluate the sterilization ability of the sterilization space of the equipment according to the test sterilization rate.

[0157] In these embodiments, the initial sterilization rate of the sterilization space of the device is determined according to the first bacterial content of the sterilization liquid in the sterilization test of the device, and the second bacterial content of the control liquid in the same storage environment as the sterilization liquid in the sterilization test. The initial sterilization rate is corrected by the test environment coefficient to obtain the test sterilization rate of the sterilization space of the device, so as to evaluate the sterilization ability of the sterilization space of the device according to the test sterilization rate. A method for evaluating the sterilization ability of a confined space such as a refrigerator is proposed. And because the initial sterilization rate of the confined space is corrected by the test environment coefficient, the evaluation of the sterilization ability of a confined space such as a refrigerator can be achieved more accurately.

[0158] The first bacterial content of the sterilization liquid in the sterilization space of the equipment during the sterilization test and the second bacterial content of the control liquid in the sterilization test in the same storage environment as the sterilization liquid are obtained. The results can be obtained at a certain time period during the test or after the test. The certain time period can be the time period with the best sterilization effect after the start of the test, for example, three hours after the start of the test, or five hours after the start of the test.

[0159] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization space of the device, including calculating the test sterilization rate in the following manner:

[0160] k=f×k0

[0161] Among them, k is the test sterilization rate, k0 is the initial sterilization rate, and f is the test environment coefficient.

[0162] In some embodiments, the initial sterilization rate is calculated as follows:

[0163] k0=(C1-C2)÷C1

[0164] Wherein, k0 is the initial sterilization rate, C1 is the second bacterial content, the unit is colony forming units (cfu) / ml (millilitre) or cfu / gram (g). C2 is the first bacterial content, the unit is cfu / ml or cfu / g.

[0165] In some embodiments, the test environment coefficient is calculated as follows:

[0166] f=f1, or, f=f2, or, f=f1×f2

[0167] Among them, f is the test environment coefficient, f1 is the time coefficient, and f2 is the space coefficient.

[0168] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization space of the device, including calculating the test sterilization rate in the following manner:

[0169] k1=f1×k0

[0170] Among them, k1 is the test sterilization rate, f1 is the time coefficient, and k0 is the initial sterilization rate.

[0171] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization space of the device, including calculating the test sterilization rate in the following manner:

[0172] k1=f2×k0

[0173] Among them, k1 is the test sterilization rate, f2 is the space coefficient, and k0 is the initial sterilization rate.

[0174] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization space of the device, including calculating the test sterilization rate in the following manner:

[0175] k1=f1×f2×k0

[0176] Among them, k1 is the test sterilization rate, f1 is the time coefficient, f2 is the space coefficient, and k0 is the initial sterilization rate.

[0177] In these embodiments, by calculating the test sterilization rate according to the time coefficient and the space coefficient, the initial sterilization rate can be adjusted according to the test time and the test space, thereby more accurately evaluating the sterilization capacity of a confined space such as a refrigerator.

[0178] The time coefficient is a coefficient calculated based on the test start time and test end time. It is used to correct the initial sterilization rate according to the time required for the test. The value range of the time coefficient is between 0 and 1.

[0179] The space coefficient is a coefficient calculated based on the volume of the confined space where the test is located. It is used to correct the initial sterilization rate based on the volume of the confined space where the test is located. The value range of the space coefficient is between 0 and 1.

[0180] In some implementations, the time factor is calculated as follows:

[0181] f1=a / (T2-T1)

[0182] Among them, f1 is the time coefficient, a is the first adjustment parameter, T2 is the time when the bacterial content is obtained in the sterilization test, and T1 is the preset time in the sterilization test.

[0183] In some embodiments, T1 is the time when the sterilizing liquid is placed into the sterilization space of the device.

[0184] In practical applications, the value of a can be 0.5 to 1. In some embodiments, the value of a is 1.

[0185] In some embodiments, the spatial coefficient is calculated as follows:

[0186] f2=b / V

[0187] Among them, f2 is the space coefficient, b is the second adjustment parameter, and V is the test volume of the sterilization space of the equipment.

[0188] In practical applications, the value of b can be 625.

[0189] In practical applications, when evaluating the sterilization capability of a refrigerator's refrigerating chamber, V may be the volume of the refrigerating chamber. When evaluating the sterilization capability of a refrigerator's freezing chamber, V may be the volume of the freezing chamber. When the refrigerating chamber includes a sealed drawer, when evaluating the sterilization capability of the refrigerator's refrigerating chamber, V may be the difference between the volume of the refrigerating chamber and the volume of the drawer.

[0190] In some embodiments, evaluating the sterilization capability of the sterilization space of the equipment according to the test sterilization rate includes:

[0191] When the tested sterilization rate is greater than or equal to the set sterilization rate, the processor determines that the sterilization space of the equipment has the sterilization capability.

[0192] When the tested sterilization rate is less than the set sterilization rate, the processor determines that the sterilization space of the equipment does not have the sterilization capability.

[0193] In some embodiments, the sterilization rate is set at 90%.

[0194] In some embodiments, the second colony information includes a second bacterial content and a second colony area parameter. The first colony information includes a first bacterial content and a first colony area parameter. The method for testing the sterilization capacity of the sterilization space of the device includes the following steps:

[0195] The processor obtains a first bacterial content of a sterilization test of a sterilization liquid set in a sterilization space of the device, and a second bacterial content of a control liquid in a sterilization test having the same storage environment as the sterilization liquid.

[0196] The processor determines an initial sterilization rate of the sterilization space of the device according to the first bacteria content and the second bacteria content.

[0197] The processor obtains a test environment coefficient of a sterilization space of the equipment.

[0198] The processor uses the test environment coefficient to correct the initial sterilization rate to obtain the test sterilization rate of the sterilization space of the equipment.

[0199] The processor obtains a first colony area parameter of a sterilization test of a sterilization liquid set in a sterilization space of the device, and a second colony area parameter of a control liquid having the same storage environment as the sterilization liquid in a sterilization test.

[0200] The processor determines a colony area parameter ratio of the first colony area parameter and the second colony area parameter.

[0201] The processor obtains evaluation parameters for the sterilization test experiment.

[0202] The processor determines a sterilization parameter of a sterilization space of the device according to the evaluation parameter and the colony area parameter ratio, so as to evaluate the sterilization capability of the sterilization space of the device according to the test sterilization rate and the sterilization parameter.

[0203] In these embodiments, by evaluating the sterilization capability of the sterilization space of the device according to the sterilization parameters and the test sterilization rate, the sterilization capability of a closed space such as a refrigerator can be evaluated more accurately.

[0204] In some embodiments, the sterilization ability of the sterilization space of the device is evaluated based on the test sterilization rate and the sterilization parameters. When the sterilization space of the device is determined to have sterilization ability based on the test sterilization rate, and when it is determined that the sterilization space of the device has obvious sterilization ability based on the sterilization parameters, the sterilization ability of the sterilization space of the device is determined to be excellent.

[0205] When it is determined that the sterilization space of the equipment has sterilization ability according to the test sterilization rate, and it is determined that the sterilization space of the equipment has no obvious sterilization ability according to the sterilization parameters, the sterilization ability of the sterilization space of the equipment is determined to be good.

[0206] When it is determined that the sterilization space of the equipment has no sterilization ability according to the test sterilization rate, and it is determined that the sterilization space of the equipment has obvious sterilization ability according to the sterilization parameters, the sterilization ability of the sterilization space of the equipment is determined to be medium.

[0207] When it is determined that the sterilization space of the equipment has no sterilization ability according to the test sterilization rate, and when it is determined that the sterilization space of the equipment has no obvious sterilization ability according to the sterilization parameters, it is determined that the sterilization ability of the sterilization space of the equipment is poor.

[0208] Among them, the first colony area parameter of the sterilization liquid set in the sterilization space of the equipment in the sterilization test, and the second colony area parameter of the control liquid in the sterilization test in the same storage environment as the sterilization liquid are obtained. It can be obtained in a certain time period during the test, or it can be obtained after the test. Among them, a certain time period can be the time period with the best sterilization effect after the start of the test, for example, three hours after the start of the test, or five hours after the start of the test.

[0209] The present disclosure also provides a method for testing the sterilization capability of a sterilization space of a device, comprising:

[0210] The processor obtains a first bacterial content of a sterilization test of a sterilization liquid set in a sterilization space of the device, and a second bacterial content of a control liquid in a sterilization test having the same storage environment as the sterilization liquid.

[0211] The processor determines an initial sterilization rate of the sterilization space of the device according to the first bacteria content and the second bacteria content.

[0212] The processor obtains the time coefficient.

[0213] The processor obtains the spatial coefficient.

[0214] The processor uses the time coefficient and the space coefficient to correct the initial sterilization rate to obtain the test sterilization rate of the sterilization space of the equipment.

[0215] The processor obtains a first colony area parameter of a sterilization test of a sterilization liquid set in a sterilization space of the device, and a second colony area parameter of a control liquid having the same storage environment as the sterilization liquid in a sterilization test.

[0216] The processor determines a colony area parameter ratio of the first colony area parameter and the second colony area parameter.

[0217] The processor obtains evaluation parameters for the sterilization test experiment.

[0218] The processor determines a sterilization parameter of a sterilization space of the device according to the evaluation parameter and the colony area parameter ratio, so as to evaluate the sterilization capability of the sterilization space of the device according to the test sterilization rate and the sterilization parameter.

[0219] In some embodiments, determining the sterilization parameter of the sterilization space of the device according to the ratio of the evaluation parameter to the colony area parameter includes calculating the sterilization parameter according to the following formula:

[0220]

[0221] Among them, f3 is the evaluation parameter, S1 is the first colony area parameter, and S2 is the second colony area parameter.

[0222] Among them, the evaluation parameter is to ensure the effective constant of the sterilization test. In conventional microbiological tests, the difference in colony count is more than 10 times, and the microbial sterilization test is considered effective. Therefore, S2÷S1 must be greater than 10. Set the value of f3 to When k is greater than or equal to 1, it indicates that effective sterilization has been performed. When k is less than 1, it indicates that effective sterilization has not been performed. In some embodiments, the evaluation parameter may be set to 0.316.

[0223] The first colony area parameter may be the value of the first colony area, or the product of the value of the first colony area and the dilution factor.

[0224] The second colony area parameter may be the value of the second colony area, or the product of the value of the second colony area and the dilution factor.

[0225] In some embodiments, evaluating the sterilization capability of the sterilization space of the device according to the sterilization parameters includes:

[0226] When the sterilization parameter is greater than or equal to the set value, the processor determines that the sterilization capacity of the sterilization space of the equipment is a first set capacity.

[0227] When the sterilization parameter is less than the set value, the processor determines that the sterilization capacity of the sterilization space of the equipment is the second set capacity.

[0228] Among them, the sterilization capacity of the first setting capacity is greater than the second setting capacity.

[0229] The set value may be 1. The first set capability may be that there is an obvious sterilization capability. The second set capability may be that there is no obvious sterilization capability.

[0230] In these embodiments, by comparing the sterilization parameters and the set values, the sterilization capacity of the sterilization space of the equipment can be determined more accurately.

[0231] In some embodiments, the first colony area parameter and the second colony area parameter are determined as follows:

[0232] S1=N1×A1,S2=N2×A2

[0233] Among them, S1 is the first colony area parameter, N1 is the first dilution factor, A1 is the first colony area score, S2 is the second colony area parameter, N2 is the second dilution factor, and A2 is the second colony area score.

[0234] The dilution factor is the dilution factor of the gradient dilution of the bacterial solution used to calculate the colony area parameter. The colony area parameter is determined by the dilution factor and the colony area score, which can take into account the impact of different dilution factors on the colony area parameter, thereby obtaining a more accurate colony area parameter.

[0235] In some embodiments, colonies of the bactericidal solution and the control solution are distributed in multiple cells.

[0236] The processor determines the first colony area score as follows:

[0237]

[0238] Among them, A1 is the first colony area score, Z1 is the first colony area weight of each cell under the area calculation scale, a1 is the first score weight of each cell under the area calculation scale, and n1 is the first total number of cells of the sample under the area calculation scale.

[0239] In practical applications, taking n1 as 5 as an example, the first colony area weights of the five cells are Z11=1, Z12=4, Z13=3, Z14=2, and Z15=1. The first score weights of the five cells are a11=1, a12=0.5, a13=0.25, a14=1, and a15=0.5. Then A1=Z11×a11+Z12×a12+Z13×a13+Z14×a14+Z15×a15=1×1+4×0.5+3×0.25+2×1+1×0.5=6.25.

[0240] In some embodiments, the processor determines the second colony area score as follows:

[0241]

[0242] Among them, A2 is the second colony area score, Z2 is the second colony area weight of each cell under the area calculation scale, a2 is the second score weight of each cell under the area calculation scale, and n2 is the second total number of cells of the sample under the area calculation scale.

[0243] In practical applications, taking n2 as 5 as an example, the second colony area weights of the five cells are Z21=1, Z22=4, Z23=3, Z24=2, and Z25=1. The first score weights of the five cells are a21=1, a22=0.5, a23=0.25, a24=1, and a25=0.5. Then A2=Z21×a21+Z22×a22+Z23×a23+Z24×a24+Z25×a25=1×1+4×0.5+3×0.25+2×1+1×0.5=6.25.

[0244] In some embodiments, the processor determines the first colony area score as follows:

[0245]

[0246] Among them, A1 is the first colony area score, Z1 is the first colony area weight of each cell under the area calculation scale, a1 is the first score weight of each cell under the area calculation scale, and n1 is the first total number of cells of the sample under the area calculation scale.

[0247] The processor determines the second colony area score as follows:

[0248]

[0249] Among them, A2 is the second colony area score, Z2 is the second colony area weight of each cell under the area calculation scale, a2 is the second score weight of each cell under the area calculation scale, and n2 is the second total number of cells of the sample under the area calculation scale.

[0250] In these embodiments, the first colony area score is determined by the first colony area weight of each cell under the area calculation scale and the first scoring weight of each cell under the area calculation scale. The second colony area score is determined according to the second colony area weight of each cell under the area calculation scale and the second scoring weight of each cell under the area calculation scale, and the colony area score is determined according to the colony area weight associated with the colony area of ​​each cell and the first scoring weight associated with the cell area. The influence of different cell areas on the colony area score is taken into account. The first colony area score and the second colony area score can be determined more accurately.

[0251] In some embodiments, the first colony area weight is determined as follows:

[0252] The processor determines a first area ratio of the colonies of the sterilizing liquid in each cell.

[0253] The processor determines a first colony area weight of a cell where a colony of the sterilizing liquid is located according to the first area ratio.

[0254] In some embodiments, the value of the first colony area weight can be determined according to the value of the first area ratio. The value of the first area ratio is proportional to the value of the first colony area weight. In some embodiments, the second colony area weight is determined as follows:

[0255] The processor determines a second area ratio of the colonies of the control bacterial solution in each cell.

[0256] The processor determines a second colony area weight of the cell where the colony of the control bacterial solution is located according to the second area ratio.

[0257] In some implementations, the value of the second colony area weight may be determined according to the value of the second area ratio, and the value of the second area ratio is proportional to the value of the second colony area weight.

[0258] In some embodiments, the first colony area weight is determined as follows:

[0259] The processor determines a first area ratio of the colonies of the sterilizing liquid in each cell.

[0260] The processor determines a first colony area weight of a cell where a colony of the sterilizing liquid is located according to the first area ratio.

[0261] The second colony area weight is determined as follows:

[0262] The processor determines a second area ratio of the colonies of the control bacterial solution in each cell.

[0263] The processor determines the second colony area weight of the cell where the colony of the sterilizing liquid is located according to the second area ratio. In these embodiments, the first colony area weight can be determined by the first area ratio. The second colony area weight can be determined by the second area ratio. Thus, the first colony area weight and the second colony area weight can reflect the colony area ratio of each cell.

[0264] In some embodiments, the first colony area weight is determined as follows:

[0265] When the first area ratio is greater than or equal to three quarters, the first colony area weight is the first area weight value.

[0266] When the first area accounts for less than three quarters and is greater than or equal to one half, the first colony area weight is the second area weight value.

[0267] When the first area accounts for less than one half and is greater than or equal to one quarter, the first colony area weight is the third area weight value.

[0268] When the first area accounts for less than one quarter, the first colony area weight is the fourth area weight value.

[0269] In some embodiments, the second colony area weight is determined as follows:

[0270] When the second area ratio is greater than or equal to three quarters, the second colony area weight is the first area weight value.

[0271] When the second area proportion is less than three quarters and greater than or equal to one half, the second colony area weight is the second area weight value.

[0272] When the second area ratio is less than one half and greater than or equal to one quarter, the second colony area weight is the third area weight value.

[0273] When the second area accounts for less than one quarter, the second colony area weight is the fourth area weight value.

[0274] In actual applications, the first area weight value is 4, the second area weight value is 3, the third area weight value is 2, and the fourth area weight value is 1.

[0275] In some implementations, the first scoring weight is determined as follows:

[0276] The processor determines a first ratio of the area of ​​each cell to the area of ​​a standard cell.

[0277] The processor determines a first scoring weight of a cell where a colony of the bactericidal liquid is located according to the first ratio.

[0278] In some implementations, the second scoring weight is determined as follows:

[0279] The processor determines a second ratio of the area of ​​each cell to the area of ​​the standard cell.

[0280] The processor determines a second scoring weight of the cell where the colony of the control bacterial solution is located according to the second ratio.

[0281] In some implementations, the first scoring weight is determined as follows:

[0282] The processor determines a first ratio of the area of ​​each cell to the area of ​​a standard cell.

[0283] The processor determines a first scoring weight of a cell where a colony of the bactericidal liquid is located according to the first ratio.

[0284] The second scoring weight is determined as follows:

[0285] The processor determines a second ratio of the area of ​​each cell to the area of ​​the standard cell.

[0286] The processor determines a second scoring weight of the cell where the colony of the control bacterial solution is located according to the second ratio.

[0287] The value of the first ratio is proportional to the first scoring weight, and the value of the second ratio is proportional to the second scoring weight.

[0288] In these embodiments, the first scoring weight is determined according to a first ratio of each cell area to the standard cell area, and the second scoring weight is determined according to a second ratio of each cell area to the standard cell area. The first scoring weight and the second scoring weight can reflect the ratio of each cell area to the standard cell area.

[0289] In practical applications, the first scoring weight can be determined as follows:

[0290] When the cell area is greater than half of the standard cell area, the first scoring weight is the first weight value.

[0291] When the cell area is less than or equal to half of the standard cell area and greater than one quarter of the standard cell area, the first scoring weight is the second weight value.

[0292] When the cell area is less than or equal to one quarter of the standard cell area, the first scoring weight is the third weight value.

[0293] In practical applications, the second scoring weight can be determined as follows:

[0294] When the cell area is greater than half of the standard cell area, the second scoring weight is the first weight value.

[0295] When the cell area is less than or equal to one-half of the standard cell area and greater than one-quarter of the standard cell area, the second scoring weight is the second weight value.

[0296] When the cell area is less than or equal to one quarter of the standard cell area, the second scoring weight is the third weight value.

[0297] In actual applications, the first weight value is 1, the second weight value is 0.5, and the third weight value is 0.2.

[0298] In some embodiments, obtaining the sterilization capacity of the sterilization space according to the first colony information and the second colony information includes:

[0299] The test sterilization rate is calculated according to the following relationship (1):

[0300] k1=f1×(C1-C2)÷C1 (1)

[0301] Wherein, k1 is the test sterilization rate, in %, C1 is the second bacterial content, in cfu / ml or cfu / g. C2 is the first bacterial content, in cfu / ml or cfu / g. f1 is the time coefficient, f1=a / t, t is the preset test time, and a is the first adjustment parameter. In practical applications, the value of a can be 0.5 to 1. In some embodiments, the value of a is 1.

[0302] In some embodiments, in combination Figure 8 As shown, the ways to obtain the sterilization capability of the sterilization space include:

[0303] S801. Obtain a first bacterial content of the sterilization test culture group and a second bacterial content of the control test culture group.

[0304] S802. Calculate and obtain the test sterilization rate according to the first bacterial content, the second bacterial content, and the time coefficient.

[0305] In some embodiments, the test sterilization rate is calculated according to the following relationship (2):

[0306] k1=f1×f2×(C1-C2)÷C1 (2)

[0307] Wherein, f2 is the space coefficient, f2=b÷V. V is the volume of the sterilization space, and b is the second adjustment parameter. In practical applications, the value of b can be 625.

[0308] In practical applications, when evaluating the sterilization capability of a refrigerator's refrigerating chamber, V may be the volume of the refrigerating chamber. When evaluating the sterilization capability of a refrigerator's freezing chamber, V may be the volume of the freezing chamber. When the refrigerating chamber includes a sealed drawer, when evaluating the sterilization capability of the refrigerator's refrigerating chamber, V may be the difference between the volume of the refrigerating chamber and the volume of the drawer.

[0309] In these embodiments, by calculating the test sterilization rate according to the time coefficient and the space coefficient, the sterilization capacity of the space of equipment such as a refrigerator can be evaluated more accurately.

[0310] The sterilization capacity of the sterilization space of the equipment is evaluated according to the sterilization rate, including:

[0311] When the tested sterilization rate is greater than or equal to the set sterilization rate, the processor determines that the sterilization space of the equipment has the sterilization capability.

[0312] When the tested sterilization rate is less than the set sterilization rate, the processor determines that the sterilization space of the equipment does not have the sterilization capability.

[0313] In some embodiments, the sterilization rate is set at 90%.

[0314] In these embodiments, a method for obtaining the sterilization capacity of the sterilization space is proposed, so as to better evaluate the sterilization capacity of the sterilization space.

[0315] In some embodiments, in combination Fig. 9 As shown, the ways to obtain the sterilization capability of the sterilization space include:

[0316] S901. Obtain a first bacterial content of the sterilization test culture group and a second bacterial content of the control test culture group.

[0317] S902. Calculate and obtain the test sterilization rate according to the first bacterial content, the second bacterial content, the time coefficient and the space coefficient.

[0318] In some embodiments, the first colony information includes a first colony area parameter, and the second colony information includes a second colony area parameter.

[0319] According to the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, including:

[0320] The sterilization parameters are calculated according to the following equation (3):

[0321]

[0322] Wherein, k3 is the sterilization parameter, f3 is the evaluation coefficient, S1 is the second colony area parameter, and S2 is the first colony area parameter.

[0323] Evaluate the sterilization capacity of the sterilization space of the equipment according to the sterilization parameters, including:

[0324] When the sterilization parameter is greater than or equal to the set value, the sterilization capacity of the sterilization space of the equipment is determined to be the first set capacity.

[0325] When the sterilization parameter is less than the set value, the sterilization capacity of the sterilization space of the equipment is determined to be the second set capacity.

[0326] Among them, the sterilization capacity of the first setting capacity is greater than the second setting capacity.

[0327] The set value may be 1. The first set capacity may be that there is an obvious sterilization capacity. The second set capacity may be that there is no obvious sterilization capacity.

[0328] In these embodiments, by comparing the sterilization parameters and the set values, the sterilization capacity of the sterilization space of the equipment can be determined more accurately.

[0329] Sterilization parameters Sterilization parameters In some embodiments, combined Fig.10 As shown, the ways to obtain the sterilization capability of the sterilization space include:

[0330] S1001. Obtain a first colony area parameter of a sterilization test culture group and a second colony area parameter of a control test culture group.

[0331] S1002. Calculate and obtain sterilization parameters according to the first colony area parameter, the second colony area parameter and the evaluation coefficient.

[0332] S1003. When the sterilization parameter is greater than or equal to 1, determine that the sterilization capacity of the sterilization space is capable of effective sterilization.

[0333] S1004. When the sterilization parameter is less than 1, it is determined that the sterilization capacity of the sterilization space is incapable of effective sterilization.

[0334] The test method for the sterilization ability of the sterilization space of the equipment provided in the embodiment of the present disclosure can achieve the following technical effects:

[0335] A test and evaluation method for the sterilization effect of the space of household appliances such as refrigerators is proposed. The method places a sterilization test sample group into the sterilization space of the device, and places a control test sample group into a control space with the same volume as the sterilization space of the device, obtains first colony information and second colony information, and obtains the sterilization capacity of the sterilization space based on the first colony information and the second colony information, thereby realizing the test and evaluation of the sterilization effect in the sterilization space of the device.

[0336] The technical solution of the embodiment of the present disclosure can be embodied in the form of a software product, which is stored in a storage medium and includes one or more instructions for enabling a computer device (which may be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method described in the embodiment of the present disclosure. The aforementioned storage medium may be a non-transient storage medium, including: a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk or an optical disk, and other media that can store program codes, or a transient storage medium.

[0337] The above description and the accompanying drawings fully illustrate the embodiments of the present disclosure so that those skilled in the art can practice them. Other embodiments may include structural, logical, electrical, process and other changes. The embodiments represent only possible changes. Unless explicitly required, separate components and functions are optional, and the order of operation may vary. The parts and features of some embodiments may be included in or replace the parts and features of other embodiments. Moreover, the words used in this application are only used to describe the embodiments and are not used to limit the claims. As used in the description of the embodiments and the claims, unless the context clearly indicates, the singular forms of "a", "an" and "the" are intended to include plural forms as well. Similarly, the term "and / or" as used in this application refers to any and all possible combinations of listings containing one or more associated ones. In addition, when used in the present application, the term "comprise" and its variants "comprises" and / or comprising refer to the presence of stated features, wholes, steps, operations, elements, and / or components, but do not exclude the presence or addition of one or more other features, wholes, steps, operations, elements, components and / or groups thereof. In the absence of further restrictions, the elements defined by the sentence "comprising a ..." do not exclude the presence of other identical elements in the process, method or device comprising the elements. In this article, each embodiment may focus on the differences from other embodiments, and the same and similar parts between the various embodiments may refer to each other. For the methods, products, etc. disclosed in the embodiments, if they correspond to the method part disclosed in the embodiments, then the relevant parts can refer to the description of the method part.

[0338] Those skilled in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software may depend on the specific application and design constraints of the technical solution. The technicians may use different methods for each specific application to implement the described functions, but such implementations should not be considered to exceed the scope of the embodiments of the present disclosure. The technicians may clearly understand that, for the convenience and simplicity of description, the specific working processes of the systems, devices and units described above may refer to the corresponding processes in the aforementioned method embodiments, and will not be repeated here.

[0339] In the embodiments disclosed herein, the disclosed methods and products (including but not limited to devices, equipment, etc.) can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units can be only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the coupling or direct coupling or communication connection between each other shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms. The units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the units may be selected according to actual needs to implement this embodiment. In addition, each functional unit in the embodiment of the present disclosure may be integrated in a processing unit, or each unit may exist physically alone, or two or more units may be integrated in one unit.

[0340] The flowchart and block diagram in the accompanying drawings show the possible architecture, function and operation of the system, method and computer program product according to the embodiment of the present disclosure. In this regard, each box in the flowchart or block diagram can represent a module, a program segment or a part of the code, and the module, the program segment or a part of the code contains one or more executable instructions for realizing the specified logical function. In some alternative implementations, the functions marked in the box can also occur in a different order from the order marked in the accompanying drawings. For example, two consecutive boxes can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, which can depend on the functions involved. In the description corresponding to the flowchart and the block diagram in the accompanying drawings, the operations or steps corresponding to different boxes can also occur in a different order from the order disclosed in the description, and sometimes there is no specific order between different operations or steps. For example, two consecutive operations or steps can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, which can depend on the functions involved. Each block in the block diagrams and / or flowcharts, and combinations of blocks in the block diagrams and / or flowcharts, may be implemented by a dedicated hardware-based system that performs the specified functions or actions, or may be implemented by a combination of dedicated hardware and computer instructions.

Claims

1. A method for testing the sterilization capacity of a sterilization space of an equipment, characterized in that: include: Prepare bacterial solution for testing; The test bacterial solution was prepared into a control bacterial solution group and a bactericidal bacterial solution group; The control bacterial solution group and the bactericidal bacterial solution group were prepared as a control test sample group and a bactericidal test sample group respectively; The sterilization test sample group is placed in the sterilization space of the equipment for sterilization test to obtain a sterilization sample group after the test; During the sterilization test, the equipment is started and operated so that the sterilization space reaches the set test conditions, and the equipment runs for the preset test time; Place the control test sample group in a control space with the same volume as the sterilization space of the equipment for control testing to obtain a post-test control sample group; during the control test, make the control space have environmental conditions consistent with the set test conditions of the sterilization test and place it for a preset test time; Performing bacterial culture on the post-test sterilization sample group and the post-test control sample group respectively to obtain a sterilization test culture group and a control test culture group; Obtaining first colony information of a sterilization test culture group and second colony information of a control test culture group; The sterilization capacity of the sterilization space is obtained according to the first colony information and the second colony information.

2. The testing method according to claim 1, characterized in that: The method of preparing the test bacterial solution comprises: The pasteurized milk is subjected to rejuvenation culture to obtain a test bacterial solution with a set colony count; wherein the rejuvenation culture includes shaking culture for a first period of time, or static culture for a second period of time.

3. The testing method according to claim 1, characterized in that: The control bacterial solution group and the sterilization bacterial solution group are respectively prepared as a control test sample group and a sterilization test sample group, including: Spread the bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group onto sterilized glass slides or sterilized filter paper sheets respectively, to obtain a first control test sample group and a first sterilization test sample group respectively; Then, the first control test sample group is subjected to a sterilization test to obtain a first post-test control sample group, and the first sterilization test sample group is subjected to a control test to obtain a first post-test sterilization sample group.

4. The testing method according to claim 3, characterized in that: Bacterial culture was performed on the sterilized sample group after the test and the control sample group after the test, including: The first post-test sterilization sample group and the first post-test control sample group are respectively placed in a second volume of physiological saline of a first concentration, and balanced at a second temperature for a third time, thereby obtaining a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group; Respectively pipette a first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample, and add them into sterile culture plates; A first volume of plate count agar medium is injected into each sterile culture plate, and then the sterile culture plate is placed in an environment of a third temperature and cultured for a fourth time period.

5. The testing method according to claim 1, characterized in that: The control bacterial solution group and the sterilization bacterial solution group are respectively prepared as a control test sample group and a sterilization test sample group, including: The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group are respectively spread on the bottom surface of the sterile culture dish or on the agar solidified layer laid on the bottom surface of the sterile culture dish, to obtain a second control test sample group and a second sterilization test sample group; Then, the second control test sample group is subjected to a sterilization test to obtain a second post-test control sample group, and the second sterilization test sample group is subjected to a control test to obtain a second post-test sterilization sample group.

6. The testing method according to claim 5, characterized in that: Bacterial culture was performed on the sterilized sample group after the test and the control sample group after the test, including: Covering the bacterial nutrient gel suspension or the plate count agar medium on the surfaces of the culture dishes of the second post-test sterilization sample group and the second post-test control sample group, respectively, and placing the bacterial nutrient gel suspension or the plate count agar medium in an environment of a fourth temperature for a sixth period of time after solidification of the bacterial nutrient gel suspension or the plate count agar medium; The bacterial nutrient gel suspension includes, by weight percentage, 0.2% to 0.6% soy protein, 0.1% to 0.8% trypsin, 0.05% to 0.45% glucose, 2% to 8% sodium chloride, 0.05% to 0.5% dipotassium hydrogen phosphate, 0.2% to 0.9% agar, and water is supplemented to 100%.

7. The testing method according to any one of claims 1 to 6, characterized in that: The first colony information includes a first bacterial content; the second colony information includes a second bacterial content; According to the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, including: The test sterilization rate is calculated according to the following relationship (1): k1=f1×(C1-C2)÷C1 (1) Among them, k1 is the test sterilization rate, the unit is %; C1 is the second bacterial content, the unit is cfu / ml or cfu / g; C2 is the first bacterial content, the unit is cfu / ml or cfu / g; f1 is the time coefficient, f1=a / (T2-T1), T2 is the moment when the bacterial content is obtained in the sterilization test, T1 is the preset moment in the sterilization test, and a is the first adjustment parameter.

8. The testing method according to claim 7, characterized in that: The test sterilization rate is calculated according to the following relationship (2): k1=f1×f2×(C1-C2)÷C1 (2) Wherein, f2 is the space coefficient, f2=b÷V; V is the test volume of the sterilization space of the equipment, and b is the second adjustment parameter.

9. The testing method according to any one of claims 1 to 6, characterized in that: The first colony information includes a first colony area parameter; the second colony information includes a second colony area parameter; According to the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, including: The sterilization parameters are calculated according to the following equation (3): Among them, k3 is the sterilization parameter; f3 is the evaluation coefficient; S1 is the second colony area parameter, and S2 is the first colony area parameter.

10. The testing method according to claim 9, characterized in that: The colony area parameter is calculated according to the following equation (4): S=N×A (4) Wherein, N is the dilution factor and A is the colony area score.

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