Microcosm test device for simulating genetic engineering blue-green algae remediation system
By designing sealing self-checking measures in the microcosm test device that simulates the genetically engineered cyanobacteria repair system, the problem of leakage in the simulation box and external interference is solved, and real-time monitoring and evaluation accuracy during the test process are achieved.
Patent Information
- Application Number
- CN202411187525.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-28
- Publication Date
- 2025-01-21
AI Technical Summary
The existing simulation boxes lack sealing self-inspection measures, which leads to the inability to detect leakage in time, and external interference affects the simulation evaluation results.
A microcosm experimental device that simulates the genetically engineered cyanobacteria repair system is designed, including a test box and a box lid. The box lid is equipped with a simulation light, the test box is equipped with a controller, and the box lid is equipped with an inner seal and a The external seal monitors the sealing performance in real time through three detection methods: gas leakage, liquid leakage and pressure block movement driven by gas through the gap.
It can timely discover the leakage of the test device, reduce external interference, and ensure the accuracy of cyanobacteria repair evaluation.
Smart Images
Figure CN119334551A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of environmental restoration simulation equipment, in particular to a microcosm test device for simulating a genetically engineered cyanobacteria restoration system. Background Art
[0002] In the field of ecological restoration and environmental governance, cyanobacteria, as an important microbial resource, has gradually attracted widespread attention due to its unique physiological characteristics and genetic engineering potential. In recent years, with the development of genetic engineering technology, cyanobacteria have shown great application potential in environmental governance, bioenergy production, drug development and other fields. In practical applications, the research on cyanobacteria genetic engineering restoration system often faces many challenges, such as low gene expression efficiency, poor environmental adaptability, and difficult to evaluate the restoration effect. In order to more accurately simulate and evaluate the performance of cyanobacteria genetic engineering restoration system under complex environmental conditions, microcosm test devices are generally used to simulate genetic engineering cyanobacteria restoration system. In the simulation process, the environment to be repaired is simulated inside the simulation box, and cyanobacteria are put into the simulation box. The internal environment of the simulation box is detected within a preset time to evaluate the cyanobacteria's ability to repair the environment. In the simulation process, the simulation box is generally separated from the outside world. However, the existing simulation box generally lacks sealing self-test measures, which leads to the failure to detect leakage in the simulation box in time, and the internal environment of the simulation box is disturbed by the outside world, affecting the simulation evaluation results. Summary of the invention
[0003] The purpose of the present invention is to provide a microcosm test device for simulating a genetically engineered cyanobacteria restoration system, so as to solve the problem that the existing simulation box proposed in the above background technology generally lacks sealing self-inspection measures, resulting in failure to timely detect leakage in the simulation box, and causing the internal environment of the simulation box to be disturbed by the outside world, thereby affecting the simulation evaluation results.
[0004] To achieve the above-mentioned object, the present invention provides the following technical solutions: A microcosm test device for simulating a genetically engineered cyanobacteria restoration system, comprising: a test box and a box cover arranged on the test box, the box cover is provided with a simulation light, the test box is provided with a controller, and the box cover is provided with an inner seal and an outer seal respectively attached to the inner and outer walls of the test box;
[0005] The test box includes an outer box, an inner box, and a support block disposed between the outer box and the inner box, and a filling liquid is filled between the outer box and the inner box;
[0006] The box cover comprises a cover plate and an elastic wrapping layer wrapped on one side of the cover plate, and gas is filled between the cover plate and the elastic wrapping layer;
[0007] Wherein, a groove is provided at the bottom of the cover plate, and a pressing block for fitting with the top wall of the test box body is slidably provided in the groove.
[0008] Preferably, a telescopic connector is provided between the cover plate and the elastic wrapping layer;
[0009] Among them, the telescopic connecting part includes a plurality of sets that are slidably connected in sequence, an elastic part is provided between two adjacent sets, a sliding groove is provided on the inner side wall of the set, a sliding block for slidingly inserting into the sliding groove is provided on the set, conductive sheets that fit each other are provided on the slider and in the sliding groove, and an indicator light that is controlled by two conductive sheets to turn on the power is provided on the cover plate.
[0010] Preferably, two protrusions are provided in the slide groove, and the two protrusions are respectively located at the front and rear sides of the moving direction of the slider.
[0011] Preferably, a plurality of the sliders and a plurality of the slide slots are provided with conductive sheets, the conductive sheets on the sliders and the conductive sheets in the slide slots form a conductive assembly, and the indicator lights are energized by controlling the plurality of conductive assemblies.
[0012] Preferably, an indicator plate located above the filling liquid is slidably provided between the outer box body and the inner box body.
[0013] Preferably, a winding assembly is provided on the outer box body, a pull rope for connecting to an indicator plate is provided on the winding end of the winding assembly, an installation groove is provided on the indicator plate, and a sensor for fitting with a cover plate and controlling the opening and closing of the winding assembly is provided in the installation groove.
[0014] Preferably, a mounting hole is provided on the side wall of the mounting groove, a limit rod and a spring member connected to the limit rod are slidably provided in the mounting hole, a limit groove is provided on the side wall of the sensor, a guide groove is provided on the inner wall of the outer box body, the end of the limit rod is inserted in the guide groove, and a push spring for pushing the sensor upward is provided in the mounting groove.
[0015] Preferably, the bottom of the cover plate is provided with two sealing grooves respectively located on the inner and outer sides of the groove, a moving block and a supporting spring connected to the moving block are slidably provided in the sealing groove, the moving block divides the sealing groove into two upper and lower cavities isolated from each other, the two upper cavities are connected to the outside through through holes, and the two lower cavities are connected with exhaust holes, and a connecting piece is provided between the moving block and the pressing block.
[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: when the box cover is damaged, the gas between the cover plate and the elastic wrapping layer will leak, causing the elastic wrapping layer to deform; when the test box is damaged, the liquid between the outer box and the inner box will leak out; if there is a gap between the test box and the box cover, gas will pass through the gap between the two and move the pressure block upward; the sealing of the test box, the box cover, and the space between the test box and the box cover is tested by the above three detection methods, so that leakage of the test device during the test can be discovered in time, reducing the impact of external interference on the test process, and ensuring the accuracy of the cyanobacteria restoration evaluation. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 This is a schematic diagram of the structure of the micro-universe test device of the present invention;
[0018] Figure 2 For the present invention Figure 1 A schematic diagram of the structure enlargement in the middle;
[0019] Figure 3 For the present invention Figure 2 A magnified schematic diagram of the structure at C in the middle;
[0020] Figure 4 For the present invention Figure 1 A magnified schematic diagram of the structure at B in the middle;
[0021] Figure 5 For the present invention Figure 4 Enlarged schematic diagram of the structure at point D in the middle.
[0022] In the figure: 1. test box; 101. outer box; 102. inner box; 103. support block; 2. box cover; 21. cover plate; 211. sealing groove; 212. moving block; 213. support spring; 214. exhaust hole; 215. through hole; 22. elastic wrapping layer; 23. telescopic connector; 231. slide groove; 232. kit; 233. slider; 234. conductive sheet; 235. bump; 3. inner seal; 4. outer seal; 5. controller; 6. indicator light; 7. simulation light; 8. winding assembly; 9. pull rope; 10. slot; 11. pressure block; 12. indicator board; 13. installation slot; 14. sensor; 15. limit slot; 16. limit rod; 17. spring member; 18. guide slot. DETAILED DESCRIPTION
[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0024] Example 1
[0025] See also Figure 1 A microcosm test device for simulating a genetically engineered cyanobacteria restoration system comprises: a test box 1 and a box cover 2, a simulation lamp 7 (LED lamp) is provided on the bottom wall of the box cover 2, a controller 5 is provided on the test box 1 (the controller 5 can be a central control device, such as a computer, and a temperature controller, a humidity controller and other devices are installed inside the test box 1. The device is controlled by a computer to simulate the temperature and humidity of the required environment inside the test box 1. Corresponding control devices can also be installed inside the test box 1 according to the requirements of the simulated environment. These processes and the equipment used belong to the prior art and are not described in detail here), an inner seal 3 and an outer seal 4 (both are made of rubber) are provided at the bottom of the box cover 2, the inner seal 3 is in contact with the inner wall of the test box 1, and the outer seal 4 is in contact with the outer wall of the test box 1, so as to increase the sealing between the box cover 2 and the test box 1.
[0026] It should be noted that the test box body 1 and the box cover 2 can be connected and fixed by bolts.
[0027] See also Figure 1 The test box 1 includes an outer box 101, an inner box 102 and a support block 103 arranged between the outer box 101 and the inner box 102. The space between the outer box 101 and the inner box 102 is filled with a filling liquid (such as water); the box cover 2 includes a cover plate 21 and an elastic wrapping layer 22 (made of rubber material). The elastic wrapping layer 22 wraps the cover plate 21 except the bottom. The space between the cover plate 21 and the elastic wrapping layer 22 is filled with gas (the gas may be air. After the space between the cover plate 21 and the elastic wrapping layer 22 is filled with gas, the elastic wrapping layer 22 will expand, and the cover plate 21 is provided with an inflation port for inflating air between the cover plate 21 and the elastic wrapping layer 22); a groove 10 is provided at the bottom of the cover plate 21, and a pressing block 11 is slidably provided in the inner cavity of the groove 10. The pressing block 11 is in contact with the top wall of the test box 1, and the shape of the groove 10 is the same as the shape of the top wall of the test box 1.
[0028] It should be noted that during the test, if the box cover 2 is damaged (such as the cover plate 21 or the elastic wrapping layer 22 is broken), the gas between the cover plate 21 and the elastic wrapping layer 22 will leak, causing the elastic wrapping layer 22 to deform; if the test box 1 is damaged (such as the outer box 101 and the inner box 102 are broken), the liquid between the outer box 101 and the inner box 102 will leak out; if there is a gap between the test box 1 and the box cover 2, gas will pass through the gap between the two and move the pressure block 11 upward. The above three methods are used to detect the sealing of the test box 1, the box cover 2, and the sealing between the test box 1 and the box cover 2 to ensure that leakage in the test device can be discovered in time, which is used to eliminate external interference during the test and ensure the accuracy of the cyanobacteria assessment.
[0029] In this implementation, as a further optimization solution, please refer to Figure 2 Two sealing grooves 211 are provided at the bottom of the cover plate 21. The shapes of the two sealing grooves 211 are the same as those of the slots 10 (the cross sections of the two sealing grooves 211 extend along the top wall of the test box 1. If the test box is rectangular, the two sealing grooves 211 are both in the shape of a square). The two sealing grooves 211 are located directly above the test box 1, and the two sealing grooves 211 are respectively located on the inner and outer sides of the slots 10. A moving block 212 is slidably provided in the inner cavity of the sealing groove 211. A supporting spring 213 is installed between the sealing groove 211 and the moving block 212. The moving block 212 divides the sealing groove 211 into two upper and lower cavities isolated from each other. The two upper cavities are connected to the outside through through holes 215, and the two lower cavities are connected with exhaust holes 214. A connecting piece is provided between the moving block 212 and the pressing block 11 (The connecting piece refers to the connecting rod); after the box cover 2 is connected to the test box body 1, it is connected to the exhaust hole 214 through an exhaust device (air pump) to extract the gas in the lower cavity of the sealing grooves 211 on both sides, so that a negative pressure environment is formed inside, and the external air pressure applies a downward force to the top of the moving block 212 through the through hole 215, so that the moving block 212 moves downward with the pressure block 11 until the pressure block 11 fits with the top wall of the test box body 1; when a gap is generated between the box cover 2 and the test box body 1, the negative pressure environment of the cavity under the sealing groove 211 is destroyed, so that the air pressure of the upper and lower cavities is balanced, so that the moving block 212 is subjected to reduced air pressure, and under the action of the supporting spring 213, the moving block 212 moves upward with the pressure block 11, and whether there is a leak between the box cover 2 and the test box body 1 is determined by observing whether the pressure block 11 moves.
[0030] It should be noted that in order to make the movement of the pressing block 11 more obvious, a touch switch is installed in the inner cavity of the slot 10. The touch switch is located above the pressing block 11. The touch switch is connected in series with the power supply and the LED lamp. When the moving block 212 moves the pressing block 11 upward, the pressing block 11 presses the touch switch to make the LED lamp light up.
[0031] In this embodiment, as a further optimized solution, please refer to Figure 1 , Figure 4 and Figure 5 A telescopic connector 23 is provided between the cover plate 21 and the elastic wrapping layer 22; the telescopic connector 23 includes a plurality of sets 232 that are slidably connected in sequence, an elastic member (spring) is provided between two adjacent sets 232, a slide groove 231 is provided on the inner side wall of the set 232, a slider 233 is provided on the set 232, the slider 233 on one set 232 is slidably inserted into the slide groove 231 of another set 232, and a conductive sheet 234 is provided on the slider 233 and in the slide groove 231, The cover plate 21 is provided with an indicator light 6 (LED light), and the indicator light 6 and the conductive sheet 234 are connected in series with an external power source; when air is inflated between the cover plate 21 and the elastic wrapping layer 22 to expand the elastic wrapping layer 22, the telescopic connector 23 will extend, and the slider 233 will slide in the slide groove 231 at this time. When the conductive sheet 234 on the slider 233 contacts the conductive sheet 234 in the slide groove 231, the indicator light 6 is powered on and lights up, and the inflation is stopped at this time; two bumps 235 are provided in the slide groove 231, The two protrusions 235 are respectively located at the front and rear sides of the moving direction of the slider 233, and are used to limit the movement of the slider 233 so that the slider 233 will not move easily and cause the two conductive sheets 234 to be misaligned; when the cover plate 21 or the elastic wrapping layer 22 is broken, causing the gas between the two to leak, the air pressure between the two is reduced, and under the action of the elastic member, the telescopic connector 23 contracts to separate the two conductive sheets 234 in contact, and at this time the indicator light 6 is powered off and stops emitting light. The staff can judge the sealing of the box cover 2 itself by judging whether the indicator light 6 is emitting light, making the detection process more convenient; when the cover plate 21 is broken and the air pressure inside the test box 1 is greater than the air pressure between the cover plate 21 and the elastic wrapping layer 22, the gas inside the test box 1 will enter between the cover plate 21 and the elastic wrapping layer 22, increasing the expansion amplitude of the elastic wrapping layer 22. At this time, the telescopic connector 23 is extended more, which will also cause the two conductive sheets 234 to be out of contact, causing the indicator light 6 to stop emitting light, thereby ensuring the accuracy of the detection.
[0032] In this embodiment, as a further optimized solution, please refer to Figure 4 and Figure 5 , a plurality of sliders 233 and a plurality of slide grooves 231 are provided with conductive sheets 234, the conductive sheets 234 on the sliders 233 and the conductive sheets 234 in the slide grooves 231 form a conductive assembly, and the indicator light 6 is controlled by a plurality of groups of conductive assemblies to be energized; when the conductive sheets 234 on all sliders 233 are in contact with the conductive sheets 234 in the slide grooves 231, the indicator light 6 will light up, so that the telescopic connecting member 23 is not shortened or extended much, but when one group of conductive sheets 234 is not in contact, the indicator light 6 will stop lighting up, so that the detection accuracy is higher.
[0033] In this embodiment, as a further optimized solution, please refer to Figure 1 , Figure 2 and Figure 3 An indicator plate 12 (the indicator plate 12 refers to a plate member) is slidably provided between the outer box body 101 and the inner box body 102. The indicator plate 12 is located above the filling liquid between the outer box body 101 and the inner box body 102. The indicator plate 12 can block the gap between the outer box body 101 and the inner box body 102 to prevent the filling liquid from leaking from the top of the test box body 1. When the outer box body 101 and the inner box body 102 are broken and the filling liquid leaks, the indicator plate 12 will move downward under the action of gravity. By observing whether the indicator plate 12 moves, it can be judged whether the test box body 1 itself is leaking, which makes it more convenient for the staff to judge whether the test box body 1 is leaking.
[0034] In this embodiment, as a further optimized solution, please refer to Figure 1 , Figure 2 and Figure 3 , a winding assembly 8 (motor) is provided on the outer box body 101, a pull rope 9 is provided on the output shaft of the motor, and the end of the pull rope 9 away from the motor is connected to the indicator plate 12; a mounting groove 13 is provided on the top wall of the indicator plate 12, and a sensor 14 (pressure sensor) is provided in the mounting groove 13, the sensor 14 is in contact with the bottom wall of the cover plate 21, and the sensor 14 is connected to the central control device installed on the test box body 1 (the sensor 14 continuously transmits information to the central control device); when the liquid between the outer box body 101 and the inner box body 102 begins to leak, causing the indicator plate 12 to move downward by a small amplitude, a gap is generated between the indicator plate 12 and the pressure block 11 at this time, so that the sensor 14 is not in contact with the pressure block 11, and the central control device detects that the sensor 14 is not in contact with the pressure block 11 by receiving the information, and then controls the motor to be powered on and started, and winds the pull rope 9 around the output shaft of the motor to pull the indicator plate 12 downward, increase the movement amplitude of the indicator plate 12, and make the leakage of the test box body 1 detected as soon as possible.
[0035] In this embodiment, as a further optimized solution, please refer to Figure 2 and Figure 3, a mounting hole is provided on the side wall of the mounting groove 13, a limiting rod 16 is slidably provided in the inner cavity of the mounting hole, a spring member 17 is installed between the mounting hole and the limiting rod 16, a mounting seat is slidably provided in the inner cavity of the mounting groove 13, a push spring is installed between the mounting seat and the mounting groove 13, the sensor 14 is installed above the mounting seat, a limiting groove 15 is provided on one side wall of the mounting seat facing the limiting rod 16, a guide groove 18 is provided on the inner side wall of the outer box body 101, and one end of the limiting rod 16 away from the mounting seat is inserted into the guide groove 18; when the test box body 1 itself does not leak, and there is a gap between the test box body 1 and the box cover 2, this When the pressure block 11 moves upward, the pressure on the sensor 14 disappears. Under the action of the push spring, the mounting seat moves up with the sensor 14 and continues to contact the bottom of the pressure block 11, so that the movement of the pressure block 11 will not cause the winding assembly 8 to be powered on; when the indicator plate 12 moves downward, the limit rod 16 will move downward until the limit rod 16 moves out of the inner cavity of the guide groove 18 (the distance between the left and right sides of the guide groove 18 gradually decreases from top to bottom). At this time, the limit rod 16 will be inserted into the limit groove to fix the mounting seat and the sensor 14, ensuring that when the indicator plate 12 moves downward actively, the winding assembly 8 will be powered on.
[0036] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A microcosm test device for simulating a genetically engineered cyanobacteria restoration system, comprising: A test box (1) and a box cover (2) disposed on the test box (1), wherein the box cover (2) is provided with a simulation lamp (7), and the test box (1) is provided with a controller (5), characterized in that: the box cover (2) is provided with an inner sealing member (3) and an outer sealing member (4) respectively in contact with the inner and outer side walls of the test box (1); The test box (1) comprises an outer box (101), an inner box (102), and a support block (103) arranged between the outer box (101) and the inner box (102); a filling liquid is filled between the outer box (101) and the inner box (102); The box cover (2) comprises a cover plate (21) and an elastic wrapping layer (22) wrapped on one side of the cover plate (21), and gas is filled between the cover plate (21) and the elastic wrapping layer (22); The bottom of the cover plate (21) is provided with a slot (10), and a pressing block (11) is slidably arranged in the slot (10) for fitting with the top wall of the test box (1).
2. A microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 1, characterized in that: A telescopic connecting piece (23) is provided between the cover plate (21) and the elastic wrapping layer (22); The telescopic connecting member (23) comprises a plurality of sets (232) which are slidably connected in sequence, an elastic member is provided between two adjacent sets (232), a sliding groove (231) is provided on the inner side wall of the set (232), a slider (233) which is used for slidingly inserting into the sliding groove (231) is provided on the set (232), a conductive sheet (234) which fits mutually is provided on the slider (233) and in the sliding groove (231), and an indicator light (6) which is powered on and controlled by two conductive sheets (234) is provided on the cover plate (21).
3. A microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 2, characterized in that: Two protrusions (235) are arranged in the sliding groove (231), and the two protrusions (235) are respectively located at the front and rear sides of the moving direction of the sliding block (233).
4. A microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 2, characterized in that: Conductive sheets (234) are provided on a plurality of the sliders (233) and a plurality of the slide grooves (231); the conductive sheets (234) on the sliders (233) and the conductive sheets (234) in the slide grooves (231) form a conductive component; and the indicator light (6) is energized by controlling the plurality of groups of conductive components.
5. The microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 1, characterized in that: An indicator plate (12) located above the filling liquid is slidably disposed between the outer box body (101) and the inner box body (102).
6. A microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 5, characterized in that: The outer box body (101) is provided with a winding assembly (8), a drawstring (9) for connecting to an indicator plate (12) is provided on the winding end of the winding assembly (8), a mounting groove (13) is provided on the indicator plate (12), and a sensor (14) for fitting with a cover plate (21) and controlling the opening and closing of the winding assembly (8) is provided in the mounting groove (13).
7. The microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 6, characterized in that: A mounting hole is provided on the side wall of the mounting groove (13), a limiting rod (16) and a spring member (17) connected to the limiting rod (16) are slidably provided in the mounting hole, a limiting groove (15) is provided on the side wall of the sensor (14), a guide groove (18) is provided on the inner side wall of the outer box body (101), an end of the limiting rod (16) is inserted into the guide groove (18), and a pushing spring for pushing the sensor (14) upward is provided in the mounting groove (13).
8. The microcosm test device for simulating a genetically engineered cyanobacteria restoration system according to claim 1, characterized in that: The bottom of the cover plate (21) is provided with two sealing grooves (211) respectively located on the inner and outer sides of the slot (10); a moving block (212) and a supporting spring (213) connected to the moving block (212) are slidably provided in the sealing groove (211); the moving block (212) divides the sealing groove (211) into two upper and lower cavities isolated from each other; the two upper cavities are connected to the outside through through holes (215); the two lower cavities are connected with air extraction holes (214); and a connecting piece is provided between the moving block (212) and the pressing block (11).