A kind of testing equipment and detection method for measuring peroxidase activity in rice bran

Abstract

The present invention relates to the technical field of enzyme activity detection, and in particular to an inspection device for measuring peroxidase activity in rice bran and a detection method thereof, comprising a frame as a carrier of the entire device and a near-infrared spectrometer for detecting rice bran enzyme activity, an electric push rod is installed on the frame, a free end of which is fixedly connected to a pressure plate for crushing rice bran, a tank body filled with a solvent and a plurality of test tube sleeves filled with rice bran are also provided on the frame, and a transposition mechanism is provided on the frame to protect the test tube sleeves from being damaged when the pressure plate squeezes the rice bran, the transposition mechanism comprises a transmission plate rotatably installed on the frame, a plurality of test tube sleeves are respectively fixed on the transmission plate, an arc plate rotatably connected to the transmission plate and matched with the test tube sleeve, and a shaking mechanism for shaking the test tube to promote mixing of rice bran and solution is also installed on the frame. The present invention can quickly detect and measure enzyme activity, reduce the time required for experiments, improve work efficiency, and is suitable for occasions requiring rapid results.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme activity detection, in particular to a testing device for measuring peroxidase activity in rice bran and a detection method thereof. Background Art

[0002] Rice bran is a byproduct of rice processing and is rich in nutrients such as vitamins, fats and dietary fiber. However, the peroxidase present in rice bran can cause it to become rancid and deteriorate during storage and processing. In order to extend the shelf life of rice bran and improve its application value in food and medicine, it is necessary to sample and test rice bran samples, and then treat the same batch of rice bran in a targeted manner based on the test results of the samples. In traditional enzyme activity detection devices, in order to improve the detection quality of rice bran enzyme activity, rice bran is usually crushed. However, during this crushing process, the direct contact between the mechanical structure and the test tube often causes damage or rupture of the test tube, thereby interrupting the experiment and causing waste of samples and reagents. Damage to the test tube not only affects the experimental process, but may also cause leakage of harmful substances and affect laboratory safety. In addition, many traditional equipment use metal test tubes. Although hard test tubes are not easily damaged, metal materials will introduce additional friction and heat during the reaction of rice bran with the solution. These factors can change the environmental conditions of the enzyme reaction, reduce the activity of the enzyme, and even lead to changes in the reaction mechanism. The contact between the test tube wall and the reactants may also affect the uniformity of the reaction, causing the concentration in some areas to be too high or too low, thus affecting the measurement results of the overall enzyme activity. Summary of the invention

[0003] In view of the shortcomings of the prior art, the present invention provides a testing device for measuring peroxidase activity in rice bran, which has the advantage of using bases made of different materials in different situations and solves the problem that the same material base affects the experiment.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] The invention discloses an inspection device for measuring peroxidase activity in rice bran, comprising a frame as a carrier of the whole device and a near-infrared spectrometer for detecting enzyme activity of rice bran. An electric push rod is installed on the frame, and the free end of the electric push rod is fixedly connected to a pressure plate for crushing rice bran. The frame is also provided with a tank body filled with a solvent and a plurality of test tube sleeves filled with rice bran. The frame is equipped with a shifting mechanism for protecting the test tube sleeves from damage when the pressure plate squeezes the rice bran. The shifting mechanism comprises a transmission plate rotatably installed on the frame, a plurality of test tube sleeves are respectively fixed on the transmission plate, an arc plate rotatably connected to the transmission plate and matched with the test tube sleeves, a bottom plate one combined with the test tube sleeves for crushing rice bran and a bottom plate two for mixing solution with rice bran are respectively provided on both sides of the arc plate. The frame is also provided with a shaking mechanism for shaking the test tubes to promote mixing of the rice bran and the solution.

[0006] Preferably, a base for supporting chassis 1 is fixedly connected to the frame, the base is located below the pressure plate, and a protective ring that fits the inner wall of the test tube sleeve is telescopically mounted on chassis 1.

[0007] Preferably, an arc ring is fixedly connected to the outer side of the second chassis, a connecting frame is provided on the arc plate, a spring 1 is fixedly connected to the connecting frame, the second chassis is fixedly connected to the spring 1, an interlayer is provided on the second chassis, a plurality of second springs are fixedly connected in the interlayer, a sealing ring is fixedly connected to the plurality of second springs, the bottom of the test tube sleeve is inserted into the interlayer, an electric telescopic rod 2 for removing the second chassis is fixedly installed on the connecting frame, and the free end of the electric telescopic rod 2 is located above the electromagnet.

[0008] Preferably, a limit rod slidably connected to the connecting frame is fixedly connected to the chassis 2, an electromagnetic strip is fixedly connected to the bottom end of the limit rod, and a magnetic sensor magnetically connected to the electromagnetic strip is fixedly mounted on the connecting frame for controlling the addition of solvent according to the amount of rice bran.

[0009] Preferably, the frame is fixed with a motor for driving the transmission plate to rotate, the transmission plate is rotatably mounted with a connecting shaft connected to a similar arc plate, the connecting shaft is fitted with a spur gear, and the frame is fixed with a plurality of racks corresponding to the spur gears.

[0010] Preferably, an electric telescopic rod 1 is fixedly mounted on the frame, a plurality of top rods corresponding to the protective rings are fixedly connected to the electric telescopic rod 1, and sliding grooves corresponding to the top rods are provided on the chassis 1 and the base.

[0011] Preferably, the shaking mechanism includes a motor fixedly mounted on an arc-shaped plate, a connecting frame fixedly connected to the free end of the motor, a load-bearing plate corresponding to a nearby test tube sleeve fixedly connected to the transmission plate, a slider slidably connected to the load-bearing plate through an arc-shaped groove, the arc-shaped groove takes the output end of the motor as the center, the test tube sleeve is hinged on the slider, and a transmission component for stirring rice bran and solution is also provided on the arc-shaped plate.

[0012] Preferably, the transmission assembly includes a cavity arranged in chassis two, on which a rotating shaft is rotatably mounted, a plurality of stirring bars in contact with rice bran and solvent are fixedly connected to the transmission shaft, and a plurality of transmission blades located in the cavity are also fixedly connected, a guide rail is fixedly connected in the cavity, a ball is slidably connected in the guide rail, and the ball contacts the transmission blade to drive the stirring bars on the rotating shaft to rotate.

[0013] Preferably, a liquid outlet pipe is fixedly connected to the tank body, an electric valve is installed on the liquid outlet pipe, a material box for collecting waste is provided on one side of the frame, a water pump is equipped on the material box, the output pipe of the water pump is connected to a guide pipe, and a nozzle is connected to the guide pipe for cleaning the test tube sleeve and the second chassis.

[0014] A method for testing a testing device for measuring peroxidase activity in rice bran comprises the following steps:

[0015] S1. Weigh a certain amount of rice bran sample and put the rice bran sample into a test tube sleeve;

[0016] S2, adding solvent to the tank body, starting the machine to move the test tube sleeve to the bottom of the tank body, and then opening the tank body to introduce the solvent into the test tube sleeve containing rice bran;

[0017] S3, mixing and reacting the crushed rice bran and the solvent in the test tube;

[0018] S4, the machine continues to run and drives the test tube sleeve to move to the bottom of the near-infrared spectrometer, the near-infrared spectrometer measures the fluorescence intensity of the reaction mixture, and calculates the activity of the enzyme in the rice bran based on the comparison of the fluorescence intensity with the known standard curve;

[0019] S5. Based on the relationship between fluorescence intensity and enzyme activity, a standard curve is drawn to conduct quantitative analysis on the samples to obtain the activity level of the enzyme in rice bran.

[0020] By means of the above technical solution, the present invention provides a testing device for measuring the activity of peroxidase in rice bran, which has at least the following beneficial effects:

[0021] 1. The testing equipment for determining the activity of peroxidase in rice bran can realize simultaneous processing of multiple test tube sets by setting a transposition mechanism and driving the transmission plate to rotate by a motor, thereby improving the experimental efficiency, making sample testing no longer be carried out individually, and supporting the needs of batch testing.

[0022] 2. The testing equipment for measuring the activity of peroxidase in rice bran, the sliding of the ball in the guide rail enables the stirring blades to function at different positions, thus maximizing the mixing effect. This unique movement mode not only increases the coverage area of ​​stirring, but also reduces the resistance to the fluid.

[0023] 3. The testing equipment for measuring the activity of peroxidase in rice bran has a magnetic sensor that can monitor the position of the electromagnetic strip. The lower the electromagnetic strip is, the more rice bran there is, and vice versa. When the test tube sleeve moves to the bottom of the liquid outlet tube, the processor controls the electric valve according to the value of the magnetic sensor to add a solution corresponding to the amount of rice bran.

[0024] 4. The testing equipment for determining the activity of peroxidase in rice bran, when the second chassis and the test tube sleeve overlap, the arc plate stops rotating, and at the same time the bottom of the test tube sleeve is inserted into the interlayer to complete the sealing of the test tube sleeve, preventing the rice bran from spilling due to shaking or rotation during operation, thereby keeping the experimental environment clean and tidy, and ensuring the accuracy of the test.

[0025] 5. In the testing equipment for determining the activity of peroxidase in rice bran, the free sliding of the sphere can be adaptively adjusted according to the left and right swing of the second chassis, ensuring that no matter how the flow state of the liquid is, the stirring bar can produce a stirring effect in time, thereby making the stirring process more flexible.

[0026] 6. The testing equipment for determining the activity of peroxidase in rice bran can quickly process freshly crushed rice bran with a solution, reducing the storage time, thereby improving the freshness of the sample and the reliability of the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention:

[0028] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention in the front view direction;

[0029] Figure 2 It is a schematic diagram of the external connection structure of the transmission plate of the present invention;

[0030] Figure 3 It is a structural schematic diagram of the transposition mechanism of the present invention;

[0031] Figure 4 It is a schematic diagram of the external connection structure of the connection frame of the present invention;

[0032] Figure 5 This is a schematic diagram of the external connection structure of the chassis 2 of the present invention;

[0033] Figure 6 It is a structural schematic diagram of the shaking mechanism of the present invention;

[0034] Figure 7 It is a rear cross-sectional view of the second chassis of the present invention.

[0035] Reference numerals:

[0036] 100, frame; 101, electric push rod; 102, pressure plate; 103, tank; 104, electric valve; 105, water pump; 106, flow guide pipe; 107, nozzle; 108, material box; 109, near-infrared spectrometer;

[0037] 200, transposition mechanism; 201, motor; 202, transmission plate; 203, arc plate; 204, chassis 1; 205, chassis 2; 206, arc ring; 207, test tube sleeve; 208, connecting shaft; 209, spring 1; 210, interlayer; 211, spring 2; 212, sealing ring; 213, spur gear; 214, protective ring; 215, electric telescopic rod 1; 216, top rod; 217, limit rod; 218, magnetic sensor; 219, electromagnetic strip; 220, electric telescopic rod 2; 221, rack; 222, base;

[0038] 300, shaking mechanism; 301, load-bearing plate; 302, slider; 303, transmission assembly; 3031, motor; 3032, connecting frame; 3033, cavity; 3034, guide rail; 3035, ball; 3036, rotating shaft; 3037, transmission blade; 3038, stirring bar. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Rice bran is a byproduct of rice processing and is rich in various nutrients and active substances, such as fat, dietary fiber, vitamins and minerals. In addition, rice bran also contains a variety of enzymes, such as amylase, protease and cellulase, which have important application value in food processing, feed production and biotechnology. Therefore, accurate determination of enzyme activity in rice bran is crucial to evaluate its nutritional value, application potential and optimize processing technology.

[0041] Traditional enzyme activity detection methods usually rely on complex experimental procedures, which require a lot of time and manpower costs, and the experimental results are greatly affected by the operator's experience and skills.

[0042] Embodiment 1:

[0043] Different materials have different reactivity to chemical reagents. Metal materials will react with reagents in specific solutions, which will interfere with the detection of enzyme activity. Therefore, it is very important to choose materials with good chemical stability. In order to solve the above problems, combined with Figure 1-Figure 3 As shown, a testing device for measuring peroxidase activity in rice bran provided by the present invention comprises a frame 100 as a carrier of the entire device and a near-infrared spectrometer 109 for detecting peroxidase activity in rice bran, an electric push rod 101 is installed on the frame 100, and its free end is fixedly connected to a pressing plate 102 for crushing rice bran, a tank body 103 filled with a solvent and a plurality of test tube sleeves 207 filled with rice bran are also provided on the frame 100, and the solvent is a buffer solution (such as phosphate buffer, pH 7.0-8.0), and a transposition mechanism 200 is provided on the frame 100 to protect the test tube sleeves 207 from being damaged when the pressing plate 102 squeezes the rice bran, which is conducive to improving the service life of the device;

[0044] In the process of crushing or mechanical processing, plastic materials may indeed be damaged. The physical properties, toughness and hardness of plastic materials are usually lower than those of materials such as metal or glass. When a strong compressive force is applied, the plastic may crack, break or deform. In order to solve the above problems, the transposition mechanism 200 includes a transmission plate 202 rotatably mounted on the frame 100, and a plurality of test tube sleeves 207 are respectively fixed on the transmission plate 202. The transmission plate 202 is rotatably connected to an arc plate 203 that cooperates with the test tube sleeves 207. A chassis 1 204 and a chassis 2 205 are provided on both sides of the arc plate 203. The chassis 1 204 is made of metal material, and the chassis 205 is made of polypropylene material. The chassis 1 204 is combined with the test tube sleeve 207 to crush rice bran. It can withstand higher pressure to ensure that the rice bran is crushed. During the process of mixing rice bran, the structure is kept stable, providing sufficient strength and durability, and the second chassis 205 is used to mix the solution with rice bran. It has good chemical stability, is not easy to react with the solution, can effectively avoid contaminating the experimental sample, and has a smooth surface, which is easy to clean and maintain. In the initial state, the first chassis 204 is located below the test tube sleeve 207 and combined with it. The electric push rod 101 starts to drive the pressure plate 102 to crush the rice bran. After the crushing is completed, the second chassis 205 is moved below the test tube sleeve 207, so that the rice bran is located in the second chassis 205, and then the solution is added into the second chassis 205 to mix it with the rice bran;

[0045] The near-infrared spectrometer 109 emits near-infrared light to the mixed rice bran. The rice bran absorbs light of a specific wavelength, and the light not absorbed is received by the detector and can be measured by transmission, reflection or diffuse reflection. By analyzing the characteristic peaks in the absorption spectrum, the chemical composition and concentration of the sample can be inferred, and then the activity state of the peroxidase in the rice bran can be determined.

[0046] The frame 100 is also provided with a shaking mechanism 300 for shaking the test tube to promote mixing of the rice bran and the solution.

[0047] When the pressure plate 102 descends, it will contact the test tube sleeve 207, and then it will also scratch the inner wall of the test tube sleeve 207. In order to solve the above problem, a base 222 for supporting the chassis 204 is fixedly connected to the frame 100. The base 222 is located below the pressure plate 102. A protective ring 214 that fits the inner wall of the test tube sleeve 207 is retractably installed on the chassis 204. When the chassis 204 and the test tube sleeve 207 are combined, the protective ring 214 rises and moves into the test tube sleeve 207, which can protect the test tube sleeve 207 and avoid damage to the test tube sleeve 207 when crushing rice bran.

[0048] According to the embodiments, by combining the advantages of metal materials and polypropylene materials and adopting a double chassis design, the performance and practicality of the equipment can be significantly improved, ensuring rapid and accurate determination of rice bran enzyme activity.

[0049] Embodiment 2:

[0050] If the design of the chassis does not take into account the sealing or fixation of the rice bran, especially when changing position, the rice bran may fall due to gravity or tilt. In order to solve the above problems, combined with Figure 2-Figure 5 As shown, on the basis of the first embodiment, an arc ring 206 is fixedly connected to the outer side of the second chassis 205, a connecting frame 3032 is provided on the arc plate 203, a spring 1 209 is fixedly connected to the connecting frame 3032, the second chassis 205 is fixedly connected to the spring 1 209, an interlayer 210 is provided on the second chassis 205, a plurality of springs 211 are fixedly connected in the interlayer 210, a sealing ring 212 is fixedly connected to the plurality of springs 211, the bottom of the test tube sleeve 207 is inserted into the interlayer 210, an electric telescopic rod 220 for removing the second chassis 205 is fixedly installed on the connecting frame 3032, the free end of the electric telescopic rod 220 is located above the electromagnet, and when the first chassis 204 and the chassis are replaced, the electric telescopic rod 220 is fixedly connected to the electromagnet. When the chassis 205 is in the second position 205, since the arc plate 203 and the chassis 1 204 are located in the same plane, the rice bran can be prevented from spilling out when the arc plate 203 rotates. Then the chassis 205 moves with the arc plate 203, and the arc ring 206 first contacts the test tube sleeve 207. Due to the spring, the arc ring 206 and the chassis 205 descend. When the chassis 205 and the test tube sleeve 207 overlap, the arc plate 203 stops rotating, and the bottom of the test tube sleeve 207 is inserted into the interlayer 210 to complete the sealing of the test tube sleeve 207, preventing the rice bran from spilling out due to shaking or rotation during operation, thereby keeping the experimental environment clean and tidy and ensuring the accuracy of the test.

[0051] Furthermore, a material box 108 for collecting waste is provided on one side of the frame 100, and a water pump 105 is provided on the material box 108. The output pipe of the water pump 105 is connected to the guide pipe 106, and the guide pipe 106 is connected to the nozzle 107 for cleaning the test tube sleeve 207 and the second chassis 205. After the rice bran is detected, the arc plate 203 rotates, the test tube sleeve 207 and the chassis 1 204 and the chassis 2 205 are separated, and the transmission plate 202 moves the chassis 1 204, the chassis 2 205 and the test tube sleeve 207 to the top of the material box 108, and then the nozzle 107 sprays water to rinse the chassis 1 204, the chassis 2 205 and the test tube sleeve 207, and the motor 3031 drives the connecting frame 3032 to rotate, and the chassis 2 205 is turned over as the connecting frame 3032 rotates, and then the residue can be dumped into the material box 108.

[0052] Embodiment three:

[0053] The amount of solution added may lead to deviations in experimental results. For example, excessive solution may dilute the reactants, resulting in low enzyme activity test results; insufficient solution may cause incomplete reactions, resulting in high results. In order to solve the above problems, combined with Figure 1-Figure 5 As shown, on the basis of the second embodiment, a liquid outlet pipe is fixedly connected to the tank body 103, an electric valve 104 is installed on the liquid outlet pipe, and a processor is also installed. A limit rod 217 slidably connected to the connecting frame 3032 is fixedly connected to the chassis 205, an electromagnetic strip 219 is fixedly connected to the bottom end of the limit rod 217, and a magnetic sensor 218 magnetically connected to the electromagnetic strip 219 is fixedly installed on the connecting frame 3032, which is used to control the addition of solvent according to the amount of rice bran. Due to the provision of a spring 1 209, the rice bran is located in the chassis 205 and is caused to descend, and the electromagnetic strip 219 on the upper limit rod 217 on the chassis 205 descends accordingly, and the magnetic sensor 218 can monitor the position of the electromagnetic strip 219. The lower the electromagnetic strip 219 is, the more rice bran the amount is, and vice versa. When the test tube sleeve 207 moves to the bottom of the liquid outlet pipe, the processor controls the electric valve 104 according to the numerical value of the magnetic sensor 218 to add a solution corresponding to the amount of rice bran.

[0054] Without precise displacement control, the sample processing process will be uneven, which may cause distortion of experimental results and affect the credibility of scientific research. The frame 100 is fixed with a motor 201 for driving the transmission plate 202 to rotate. The transmission plate 202 is rotatably mounted with a connecting shaft 208 connected to the adjacent arc-shaped plate 203. The connecting shaft 208 is provided with a spur gear 213. The frame 100 is fixed with a plurality of racks 221 corresponding to the spur gears 213. In the process of detecting the activity of rice bran enzyme, the motor 201 is started to drive the transmission plate 202 to rotate, and a single plurality of test tube sleeves 207 and their supporting parts rotate with the transmission plate 202, so that multiple rice bran samples can be continuously detected. Subsequently, when the spur gear 213 contacts the rack 221, the connecting shaft 208 can be driven to rotate, and the transmission plate 202 rotates with the connecting shaft 208, and the chassis 1 204 and the chassis 2 205 on the transmission plate 202 are swapped, so as to achieve different treatments of rice bran.

[0055] Furthermore, an electric telescopic rod 215 is fixedly installed on the frame 100, and a plurality of top rods 216 corresponding to the protective ring 214 are fixedly connected to the electric telescopic rod 215. The chassis 204 and the base 222 are both provided with sliding grooves corresponding to the top rods 216. When the electric telescopic rod 215 is started, its free end extends to drive the top rod 216 to rise, and the top rod 216 pushes the sealing ring 212 to enter the inside of the test tube sleeve 207. After being crushed, the free end of the electric telescopic rod 215 contracts to drive the top rod 216 to descend, and then the sealing ring 212 automatically descends into the chassis 204.

[0056] According to the embodiment, the motor 201 drives the transmission plate 202 to rotate, so that multiple test tube sleeves 207 can be processed simultaneously, thereby improving the experimental efficiency, making sample testing no longer performed individually, and supporting the needs of batch testing.

[0057] Embodiment 4:

[0058] Insufficient contact between the solution and the rice bran may lead to uneven reaction. The rice bran in some areas may not fully react with the enzyme or reagent, thus affecting the overall reaction effect. In order to solve the above problems, combined with Figure 1 , Figure 4 , Figure 6 and Figure 7 As shown, on the basis of Example 3, the shaking mechanism 300 includes a motor 3031 fixedly mounted on the arc plate 203, a connecting frame 3032 fixedly connected to the free end of the motor 3031, a load-bearing plate 301 corresponding to the adjacent test tube sleeve 207 is fixedly connected to the transmission plate 202, a slider 302 is slidably connected to the load-bearing plate 301 through an arc groove, the test tube sleeve 207 is hinged on the slider 302, and the arc groove takes the output end of the motor 3031 as the center. The motor 3031 is started to rotate left and right, and then can drive the chassis 205 on the connecting frame 3032 to swing left and right, and the test tube sleeve 207 swings with the chassis 205, which can improve the mixing effect of the rice bran and the solution, and is also provided with a transmission component 303 for stirring the rice bran and the solution, which further promotes the mixing of the rice bran and the solution, increases the kinetic energy of the fluid, creates good conditions for the full reaction of the sample, and ensures the accuracy of the rice bran enzyme activity detection.

[0059] Mechanical stirring may generate frictional heat, causing the mixture temperature to increase. High temperature may affect the stability of enzymes or other biologically active ingredients, and even cause loss of activity. In order to solve the above problem, the transmission component 303 includes a cavity 3033 arranged in the chassis 205, and a rotating shaft 3036 is rotatably installed on the chassis 205. A plurality of stirring bars 3038 in contact with rice bran and solvent are fixedly connected to the transmission shaft, and a plurality of transmission blades 3037 located in the cavity 3033 are also fixedly connected. A guide rail 3034 is fixedly connected in the cavity 3033, and a ball 3035 is slidably connected in the guide rail 3034. The chassis 205 swings left and right, so that the ball 3035 moves left and right in the guide rail 3034. The ball 3035 contacts the transmission blade 3037 to drive the stirring bar 3038 on the rotating shaft 3036 to rotate, so that the solution and rice bran can be stirred and mixed, which simplifies the complex mechanical layout required for conventional agitators, realizes multiple functions through a single motion mechanism (swing of the chassis), and reduces mechanical complexity and maintenance difficulty.

[0060] A multi-level stirring system is created by combining the rotating stirring bars 3038 with the spheres 3035 moving in the cavity 3033. The movement of the spheres 3035 drives the transmission blades 3037 to rotate, which in turn drives the stirring bars 3038 to achieve mixing, thereby improving the efficiency and uniformity of mixing.

[0061] It can be known from the above embodiment that: first, the chassis 1 204 and the test tube sleeve 207 are combined, and the rice bran and the substrate solvent are injected into the test tube sleeve 207, the substrate solvent is a hydrogen peroxide substrate (such as aniline, o-phenylenediamine), then the motor 201 is started to drive the transmission plate 202 to rotate, and the single or multiple test tube sleeves 207 and their supporting parts rotate with the transmission plate 202, and then the electric push rod 101 is started to drive the pressure plate 102 to crush the rice bran. After the crushing is completed, the positions of the chassis 1 204 and the chassis 2 205 are exchanged, the chassis 2 205 and the test tube sleeve 207 are combined, and then the solution is added to the chassis 2 205 to mix them, and then the chassis 2 205 continues to move to the near-infrared spectrometer 109, and then the near-infrared spectrometer 109 detects the mixed rice bran, and then the staff can check the detection data.

[0062] It should be noted that the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A testing device for measuring peroxidase activity in rice bran, comprising a frame (100) as a carrier of the entire device and a near infrared spectrometer (109) for detecting peroxidase activity in rice bran, characterized in that: An electric push rod (101) is mounted on the frame (100), the free end of which is fixedly connected to a pressing plate (102) for crushing rice bran. A tank (103) filled with a solvent and a plurality of test tube sleeves (207) filled with rice bran are also provided on the frame (100). A shifting mechanism (200) is provided on the frame to protect the test tube sleeves (207) from being damaged when the pressing plate (102) squeezes the rice bran. The shifting mechanism (200) comprises a transmission plate (202) rotatably mounted on a frame (100), a plurality of test tube sleeves (207) respectively fixed on the transmission plate (202), an arc-shaped plate (203) rotatably connected to the transmission plate (202) and matched with the test tube sleeves (207), a first chassis (204) for crushing rice bran combined with the test tube sleeves (207) being provided on both sides of the arc-shaped plate (203), the arc-shaped plate (203) and the first chassis (204) being located on the same plane, and a second chassis (205) for mixing a solution with rice bran, the first chassis (204) being made of a metal material, and the second chassis (205) being made of a polypropylene material; The frame (100) is also provided with a shaking mechanism (300) for shaking the test tube to promote mixing of the rice bran and the solution; The shaking mechanism (300) comprises a motor (3031) fixedly mounted on the arc-shaped plate (203); a connecting frame (3032) fixedly connected to the free end of the motor (3031); a bearing plate (301) corresponding to a nearby test tube sleeve (207) fixedly connected to the transmission plate (202); a slider (302) slidably connected to the bearing plate (301) via an arc-shaped groove; the arc-shaped groove takes the output end of the motor (3031) as the center of a circle; the test tube sleeve (207) is hingedly connected to the slider (302); and a transmission component (303) for stirring rice bran and a solution is also provided on the arc-shaped plate (203).

2. The testing device for measuring peroxidase activity in rice bran according to claim 1, characterized in that: A base (222) for supporting the first chassis (204) is fixedly connected to the frame (100). The base (222) is located below the pressure plate (102). A protective ring (214) that fits the inner wall of the test tube sleeve (207) is telescopically mounted on the first chassis (204).

3. The testing device for measuring peroxidase activity in rice bran according to claim 1, characterized in that: An arc ring (206) is fixedly connected to the outer side of the second chassis (205), a spring (209) is fixedly connected to the connecting frame (3032), the second chassis (205) is fixedly connected to the spring (209), an interlayer (210) is provided on the second chassis (205), a plurality of second springs (211) are fixedly connected to the interlayer (210), a sealing ring (212) is fixedly connected to the plurality of second springs (211), the bottom of the test tube sleeve (207) is inserted into the interlayer (210), an electric telescopic rod (220) for removing the second chassis (205) is fixedly installed on the connecting frame (3032), and the free end of the electric telescopic rod (220) is located above the electromagnet.

4. The testing device for measuring peroxidase activity in rice bran according to claim 3, characterized in that: A limit rod (217) slidably connected to the connecting frame (3032) is fixedly connected to the second chassis (205); an electromagnetic strip (219) is fixedly connected to the bottom end of the limit rod (217); and a magnetic sensor (218) magnetically connected to the electromagnetic strip (219) is fixedly mounted on the connecting frame (3032) for controlling the addition of solvent according to the amount of rice bran.

5. The testing device for measuring peroxidase activity in rice bran according to claim 4, characterized in that: The frame (100) is fixedly provided with a motor (201) for driving the transmission plate (202) to rotate; a connecting shaft (208) connected to the adjacent arc-shaped plate (203) is rotatably provided on the transmission plate (202); a spur gear (213) is sleeved on the connecting shaft (208); and a plurality of racks (221) corresponding to the spur gears (213) are fixedly provided on the frame (100).

6. The testing device for measuring peroxidase activity in rice bran according to claim 2, characterized in that: The frame (100) is fixedly provided with an electric telescopic rod (215), a plurality of top rods (216) corresponding to the protective ring (214) are fixedly connected to the electric telescopic rod (215), and sliding grooves corresponding to the top rods (216) are provided on the chassis (204) and the base (222).

7. The testing device for measuring peroxidase activity in rice bran according to claim 1, characterized in that: The transmission assembly (303) comprises a cavity (3033) disposed in the second chassis (205); a rotating shaft (3036) is rotatably mounted on the second chassis (205); a plurality of stirring bars (3038) in contact with rice bran and a solvent are fixedly connected to the transmission shaft; a plurality of transmission blades (3037) located in the cavity (3033) are also fixedly connected; a guide rail (3034) is fixedly connected in the cavity (3033); a ball (3035) is slidably connected in the guide rail (3034); the ball (3035) contacts the transmission blades (3037) to drive the stirring bars (3038) on the rotating shaft (3036) to rotate.

8. The testing device for measuring peroxidase activity in rice bran according to claim 1, characterized in that: The tank body (103) is fixedly connected with a liquid outlet pipe, and an electric valve (104) is installed on the liquid outlet pipe. A material box (108) for collecting waste is provided on one side of the frame (100). A water pump (105) is provided on the material box (108). The output pipe of the water pump (105) is connected to a guide pipe (106), and the guide pipe (106) is connected to a nozzle (107) for cleaning the test tube sleeve (207) and the second chassis (205).

9. A method for testing the peroxidase activity in rice bran according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1, weighing a certain amount of rice bran sample, and placing the rice bran sample into a test tube sleeve (207); S2, adding solvent to the tank body (103), starting the machine to move the test tube sleeve (207) to the bottom of the tank body (103), and then opening the tank body (103) to introduce the solvent into the test tube sleeve (207) containing rice bran; S3, the crushed rice bran and the solvent are mixed and reacted in the test tube sleeve (207); S4, the machine continues to operate, driving the test tube sleeve (207) to move to the bottom of the near-infrared spectrometer (109), and the near-infrared spectrometer (109) measures the fluorescence intensity of the reaction mixture, and calculates the activity of the enzyme in the rice bran based on the comparison of the fluorescence intensity with a known standard curve; S5. Based on the relationship between fluorescence intensity and enzyme activity, a standard curve is drawn to conduct quantitative analysis on the samples to obtain the activity level of the enzyme in rice bran.

Images