A sample injection device for capillary isotachophoresis instrument

Abstract

The present invention discloses a sample injection device for a capillary isotachophoresis instrument, which relates to the technical field of sample injection detection of electrophoresis instruments, and includes: an electrophoresis unit, including an electrophoresis operating part, an electrophoresis power supply arranged inside the electrophoresis operating part, and an electrophoresis connecting line arranged on the electrophoresis power supply, and a sample injection detection unit. Through the sample injection component and the recovery component, the solution is accurately injected and guided, and the electrophoresis power supply is controlled by the conductive part and the electrophoresis connecting line to start the electrophoresis. The pressure cover and the electrophoresis comb make the experimental observation and sample separation more convenient and uniform, and by directly injecting the solution into the solution box, the solution contamination is avoided, and the accuracy of the detection result is ensured. The recovery component uniformly processes the detection solution after the detection is completed, prevents the sample from flowing back, avoids the failure of the experiment, improves the efficiency, accuracy and environmental protection of the experiment, and reduces the waste of resources.

Description

Technical Field

[0001] The invention relates to the technical field of electrophoresis instrument sample injection detection, in particular to a sample injection device of a capillary isotachophoresis instrument. Background Art

[0002] Capillary electrophoresis is a separation and analysis technology developed in recent years. It separates ions, molecules or charged particles in the sample entering the capillary by applying a high voltage of 10 to 30 kV at both ends of the capillary. It has the advantages of high efficiency, rapidity and small injection volume. At present, capillary electrophoresis has been widely used in the separation and analysis of biological molecules such as amino acids, peptides, proteins, nucleic acids, drugs, sugars and oligosaccharides, polymers and particles.

[0003] When capillary electrophoresis is running, both ends of the capillary are immersed in the solution. Due to the need for injection, the injection end of the capillary will switch between different solutions. However, during capillary electrophoresis, only a single solution bottle is placed at both ends of the capillary to replace the solution bottle. When replacing, the instrument needs to be opened manually to manually replace the internal solution bottle. The whole process is cumbersome. Manually changing the solution can easily lead to solution contamination, resulting in abnormal test results. At the same time, after the test is completed, the test solution cannot be uniformly collected and processed, resulting in reverse outflow of the sample, causing the experiment to fail. Summary of the invention

[0004] In view of the above problems existing in the sample injection device of the existing capillary isotachophoresis instrument, the present invention is proposed.

[0005] Therefore, the present invention provides a sample injection device for a capillary isotachophoresis instrument, the purpose of which is to solve the problem that manual solution replacement can easily lead to solution contamination, thereby causing abnormal test results, and after the test is completed, the test solution cannot be collected and processed uniformly, resulting in reverse outflow of the sample and causing the experiment to fail.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: an electrophoresis unit comprises an electrophoresis operating member, an electrophoresis power source arranged inside the electrophoresis operating member, and an electrophoresis connecting line arranged on the electrophoresis power source;

[0007] The sampling detection unit comprises a sampling assembly arranged at the other end of the electrophoresis connecting line, and a recovery assembly arranged at the bottom of the sampling assembly.

[0008] As a preferred solution of the sample injection device of the capillary isotachophoresis instrument described in the present invention, the sample injection assembly includes a conductive part arranged at the other end of the electrophoresis connecting line, a solution box arranged at the bottom of the conductive part, a liquid inlet arranged at the top of the solution box, a filter plate arranged inside the solution box, a rotating shaft rotatably arranged inside the solution box, and a pressure cover arranged at the other end of the rotating shaft, and the pressure cover is rotatably connected to the solution box.

[0009] As a preferred solution of the sample injection device of the capillary isotachophoresis apparatus of the present invention, an electrophoresis rod is arranged at the bottom of the conductive member, and the electrophoresis rod penetrates and is connected to the solution box.

[0010] As a preferred solution of the sample injection device of the capillary isotachophoresis apparatus of the present invention, a liquid discharge port is arranged on one side of the bottom of the solution box, and a liquid guide port is arranged on the other side of the bottom of the solution box.

[0011] As a preferred solution of the sample injection device of the capillary isotachophoresis apparatus of the present invention, a slide groove is provided on the outer diameter of one side of the solution box, a detection area is slidably connected inside the slide groove, and one side of the detection area is penetrated and connected to the liquid guide port.

[0012] As a preferred solution of the sample injection device of the capillary isotachophoresis apparatus of the present invention, an electrophoresis comb is slidably arranged inside the detection zone, and the electrophoresis comb is slidably connected to the solution box.

[0013] As a preferred solution of the sample injection device of the capillary isotachophoresis apparatus of the present invention, a limit buckle is slidably provided on the top of the solution box, and the limit buckle is slidably connected to the pressure cover.

[0014] As a preferred solution of the sample injection device of the capillary isotachophoresis apparatus of the present invention, a transparent plate is arranged inside the gland, and a mounting plate is arranged at the bottom of the solution box.

[0015] As a preferred solution of the sample injection device of the capillary isotachophoresis instrument described in the present invention, the recovery component includes an anti-reflection bottom plate arranged at the bottom of the solution tank, a glass electrophoresis cover slidably arranged inside the anti-reflection bottom plate, and the glass electrophoresis cover is connected to the conductive part.

[0016] As a preferred solution of the sample injection device of the capillary isotachophoresis instrument described in the present invention, a waste liquid port is provided on one side of the glass electrophoresis cover, and the waste liquid port cooperates with the liquid discharge port, a liquid inlet tank is provided on the top of the glass electrophoresis cover, and the liquid inlet tank is slidably connected to the liquid inlet.

[0017] The beneficial effects of the present invention are as follows: through the injection component and the recovery component, rapid installation of the experiment, accurate solution injection and diversion, automatic electrophoresis start-up, intuitive observation and sample separation, environmentally friendly recovery, and prevention of pollution and experimental failure are achieved. The experimental efficiency is improved by quickly unfolding the injection component, ensuring that solutions a and b accurately flow into the detection area through the liquid guide port, and the electrophoresis power supply is controlled by the conductive member and the electrophoresis connection line to start electrophoresis. The pressure cover and the electrophoresis comb make the experimental observation and sample separation more convenient and uniform, and by directly injecting the solution into the solution box, the solution contamination is avoided, and the accuracy of the test results is ensured. The recovery component uniformly processes the test solution after the test is completed to prevent the sample from flowing back. It can be widely used in multiple fields such as biomolecular analysis, drug analysis, and environmental monitoring. Through the designs of accurate solution injection and diversion, automatic electrophoresis start-up, intuitive observation and sample separation, the experimental efficiency and data accuracy are significantly improved. At the same time, the device reduces resource waste and environmental pollution through the environmentally friendly recovery and pollution prevention mechanism, improves the safety of the experiment and the durability of the equipment, avoids experimental failure, improves the efficiency, accuracy and environmental protection of the experiment, and reduces resource waste. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 The figure is a schematic diagram of the overall structure of the sample injection device of the capillary isotachophoresis apparatus of the present invention.

[0020] Figure 2 The figure is a schematic diagram of the installation structure of the sample injection device of the capillary isotachophoresis apparatus of the present invention.

[0021] Figure 3 The figure is a schematic diagram of the internal structure of the sample injection device of the capillary isotachophoresis apparatus of the present invention.

[0022] Figure 4 The figure is a schematic diagram of the surface cross-sectional structure of the sample injection device of the capillary isotachophoresis apparatus of the present invention.

[0023] Figure 5 The sample injection device of the capillary isotachophoresis apparatus of the present invention Figure 4 A is an enlarged structural diagram of FIG.

[0024] Figure 6 The sample injection device of the capillary isotachophoresis apparatus of the present invention Figure 4 Schematic diagram of the enlarged structure at B.

[0025] Figure 7 The sample injection device of the capillary isotachophoresis apparatus of the present invention Figure 4 Enlarged structural diagram at C.

[0026] Figure 8 It is a schematic diagram of the fully expanded structure of the sample injection device of the capillary isotachophoresis apparatus of the present invention.

[0027] Fig. 9 The sample injection device of the capillary isotachophoresis apparatus of the present invention Figure 8 Enlarged structural diagram at D.

[0028] Fig.10 The schematic diagram of the expanded cross-sectional structure of the sample injection device of the capillary isotachophoresis apparatus of the present invention is

[0029] Explanation of the reference numerals: 100, electrophoresis unit; 101, electrophoresis operating part; 102, electrophoresis power supply; 103, electrophoresis connecting line; 200, injection detection unit; 201, injection assembly; 201a, conductive part; 201a-1, electrophoresis rod; 201b, solution box; 201b-1, liquid discharge port; 201b-2, liquid guide port; 201b-3, mounting plate; 201c, liquid inlet; 201d, filter plate; 201e, rotating shaft; 201f, pressure cover; 201f-1, transparent plate; 201g, limit buckle; 201h, slide; 201l, detection area; 201i, electrophoresis comb; 202, recovery assembly; 202a, anti-reflection bottom plate; 202b, glass electrophoresis cover; 202c, waste liquid port; 202d, liquid inlet tank. DETAILED DESCRIPTION

[0030] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0031] Example 1, reference Figure 1 - Figure 4 , which is the first embodiment of the present invention, provides a sample injection device for a capillary isotachophoresis instrument, and the device includes: an electrophoresis unit 100, and a sample injection detection unit 200.

[0032] The electrophoresis unit 100 includes an electrophoresis operating member 101, an electrophoresis power source 102 disposed inside the electrophoresis operating member 101, and an electrophoresis connection line 103 disposed on the electrophoresis power source 102. When electrophoresis detection of the capillary is required, the detection solution is injected into the interior of the injection component 201, and the sample to be detected is also injected into the interior of the injection component 201, the injection component 201 and the electrophoresis connection line 103 are connected, and the electrophoresis power source 102 is controlled to perform electrophoresis detection on the capillary sample inside the injection component 201;

[0033] The sampling detection unit 200 includes a sampling assembly 201 arranged at the other end of the electrophoresis connection line 103, and a recovery assembly 202 arranged at the bottom of the sampling assembly 201. Before the capillary electrophoresis experiment is performed, the sampling assembly 201 is taken out and the sampling assembly 201 is unfolded and installed so that the sampling assembly 201 can be detected. At the same time, the operator uses a syringe to inject the solution required for electrophoresis into the interior of the solution box 201b through the liquid inlet 201c. At the same time, the solution in the solution box 201b is filtered and divided by the sampling assembly 201 and guided to the interior of the detection area 201l for electrophoresis pretreatment. At the same time, the electrophoresis comb 201i is slidably inserted into the interior of the solution box 201b. The capillary sample is injected into the detection area 201l. After the injection of the solution and the sample is completed, the recovery component 202 is completely engaged on the surface of the injection component 201, the conductive part 201a and the electrophoresis connection line 103 are connected, and then the electrophoresis power supply 102 is controlled to start detecting the capillary sample inside the injection component 201. After the detection is completed, the injection component 201 can uniformly guide the internal detection sample and solution, uniformly collect them and then discharge them together to the designated location, ensuring that the equipment and the experimental environment are clean and tidy after the experiment. At the same time, during the electrophoresis experiment, the recovery component 202 can also effectively prevent the detection sample from flowing back, so as to prevent the sample from flowing out in reverse, resulting in incorrect experimental results.

[0034] During use, when it is necessary to perform electrophoresis detection on the capillary, the detection solution is injected into the interior of the injection component 201, and the sample to be detected is also injected into the interior of the injection component 201, the injection component 201 and the electrophoresis connection line 103 are connected, and the electrophoresis power supply 102 is controlled to perform electrophoresis detection on the capillary sample inside the injection component 201. Before the detection test starts, the injection component 201 is taken out, and the injection component 201 is unfolded and installed so that the injection component 201 can be detected. At the same time, the operator uses a syringe to inject the solution required for electrophoresis into the interior of the solution box 201b through the liquid inlet 201c. At the same time, the solution inside the solution box 201b is filtered and segmented by the injection component 201, and guided to the interior of the detection area 201l for pretreatment before electrophoresis. The electrophoresis comb 201i is slidably inserted into the interior of the solution box 201b, and the capillary sample is injected into the interior of the detection area 201l. After the injection of the solution and the sample is completed, the recovery component 202 is completely engaged on the surface of the injection component 201, the conductive part 201a and the electrophoresis connection line 103 are connected, and then the electrophoresis power supply 102 is controlled to start detecting the capillary sample inside the injection component 201. After the detection is completed, the injection component 201 can uniformly guide the internal detection sample and solution, uniformly collect them and then discharge them together to the designated location, to ensure that the equipment and the experimental environment are clean and tidy after the experiment. At the same time, during the electrophoresis experiment, the recovery component 202 can also effectively prevent the detection sample from being processed to prevent the sample from flowing back, thereby ensuring the effectiveness and accuracy of the experimental detection.

[0035] Example 2, reference Figure 1 - Fig.10, which is the second embodiment of the present invention. This embodiment is different from the first embodiment in that the sample injection assembly 201 includes a conductive member 201a disposed at the other end of the electrophoresis connection line 103, a solution box 201b disposed at the bottom of the conductive member 201a, a liquid inlet 201c disposed at the top of the solution box 201b, a filter plate 201d disposed inside the solution box 201b, a rotating shaft 201e rotatably disposed inside the solution box 201b, and a filter plate 201d disposed inside the solution box 201b. The pressure cover 201f at the other end is rotatably connected to the solution box 201b. Before performing electrophoresis experiment on the capillary, first push the pressure covers 201f on both sides to the bottom, fully open the pressure covers 201f on both sides, insert the electrophoresis comb 201i and the detection area 201l from the upper side in turn and place them at the bottom. The solution box 201b clamps the pressure covers 201f on both sides, and at the same time, slide the limit buckle 201g into the interior of the pressure cover 201f, so that the pressure cover 201f and the solution box 201b are in a closed position. into a whole, use a syringe to slowly inject the prepared gel solution into the middle gel chamber of the two detection areas 201l to the optimal position of the gel chamber, then use double distilled water or anhydrous ethanol to evenly add it to the gel chamber back and forth to make it seal, and inject the detection solution into the interior of the solution box 201b through the liquid inlet 201c, the injected solution is filtered and divided by the filter plate 201d, flows to the bottom of the solution box 201b, and starts to flow into the interior of the detection area 201l along the liquid guide port 201b-2 to contact the sample, at the same time, the conductive member 201a is connected to the electrophoresis connecting line 103, and the electrophoresis power supply 102 is controlled to start to transmit current to the conductive member 201a and the electrophoresis rod 201a-1, and the sample inside the detection area 201l is subjected to electrophoresis detection. When performing electrophoresis detection, the electrophoresis comb 201i evenly separates the internal sample to ensure the detection of the internal capillary sample, thereby making the experimental effect more accurate.

[0036] Compared with Example 1, further, an electrophoresis rod 201a-1 is provided at the bottom of the conductive member 201a, and the electrophoresis rod 201a-1 penetrates and is connected to the solution box 201b, a discharge port 201b-1 is provided on one side of the bottom of the solution box 201b, and a liquid guide port 201b-2 is provided on the other side of the bottom of the solution box 201b, a slide groove 201h is provided on the outer diameter of one side of the solution box 201b, and the inside of the slide groove 201h is slidably connected with the detection area 201l, and one side of the detection area 201l penetrates and is connected with the liquid guide port 201b-2, an electrophoresis comb 201i is slidably provided inside the detection area 201l, and the electrophoresis comb 201i is slidably connected to the solution box 201b, a limit buckle 201g is slidably provided on the top of the solution box 201b, and the limit buckle 201g is slidably connected to the pressure cover 201f, and a transparent plate 201f- is provided inside the pressure cover 201f. 1. A mounting plate 201b-3 is provided at the bottom of the solution box 201b. After the capillary electrophoresis experiment in the detection area 201l is completed, a transparent plate 201f-1 is used to observe the experimental situation in the detection area 201l, and the solutions in the detection area 201l and the solution box 201b are transported out through the liquid guide port 201b-2 and the liquid discharge port 201b-1 for unified collection and processing. The experimental efficiency is improved by quickly unfolding the injection assembly 201 to ensure that the solution accurately flows into the detection area 201l through the liquid inlet 201c, and the electrophoresis power supply 102 is controlled by the conductive member 201a and the electrophoresis connecting line 103 to start the electrophoresis. The pressure cover 201f and the electrophoresis comb 201i make the experimental observation and sample separation more convenient and uniform, and the solution is directly injected into the solution box 201b to avoid solution contamination, thereby ensuring the accuracy of the test results.

[0037] During use, before conducting an electrophoresis experiment on the capillary, first push the pressure covers 201f on both sides to the bottom, fully open the pressure covers 201f on both sides, insert the electrophoresis comb 201i and the detection area 201l from the upper side in turn and place them to the bottom, the solution box 201b clamps the pressure covers 201f on both sides, and at the same time, slide the limit buckle 201g into the interior of the pressure cover 201f, so that the pressure cover 201f and the solution box 201b form a whole, use a syringe to slowly inject the prepared gel solution into the middle gel chamber of the two detection areas 201l to the optimal position of the gel chamber, and then use double distilled water or anhydrous ethanol to evenly add it into the gel chamber back and forth to make it seal. The detection solution is injected into the solution box 201b through the liquid inlet 201c. The injected solution is filtered and divided by the filter plate 201d, flows to the bottom of the solution box 201b, and starts to flow into the detection area 201l along the liquid guide port 201b-2 to contact the sample. At the same time, the conductive member 201a is connected to the electrophoresis connecting line 103, and the electrophoresis power supply 102 is controlled to start transmitting current to the conductive member 201a and the electrophoresis rod 201a-1. The sample in the detection area 201l is subjected to electrophoresis detection. When the electrophoresis detection is performed, the electrophoresis comb 201i evenly separates the internal sample.

[0038] After the capillary electrophoresis experiment in the detection area 201l is completed, the transparent plate 201f-1 is used to observe the experimental situation in the detection area 201l, and the solutions in the detection area 201l and the solution box 201b are transported out through the liquid guide port 201b-2 and the liquid discharge port 201b-1 for unified collection and processing. The experimental efficiency is improved by quickly unfolding the injection component 201 to ensure that the solution accurately flows into the detection area 201l through the liquid inlet 201c, and the electrophoresis power supply 102 is controlled by the conductive part 201a and the electrophoresis connecting line 103 to start the electrophoresis. The pressure cover 201f and the electrophoresis comb 201i make the experimental observation and sample separation more convenient and uniform, and by directly injecting the solution into the solution box 201b, the solution contamination is avoided, ensuring the detection of the internal capillary sample, so that the experimental effect is more accurate.

[0039] The remaining structures are the same as those of Example 1.

[0040] Example 3, reference Figure 1 - Figure 5, which is the third embodiment of the present invention. This embodiment is different from the second embodiment in that: the recovery component 202 includes an anti-reflection bottom plate 202a arranged at the bottom of the solution box 201b, and a glass electrophoresis cover 202b slidably arranged inside the anti-reflection bottom plate 202a, and the glass electrophoresis cover 202b is connected to the conductive member 201a. After the capillary sample is injected into the detection area 201l, it is mixed with the solution inside the solution boxes 201b on both sides. The connection between the electrophoresis connecting line 103 and the conductive member 201a allows the current of the electrophoresis connecting line 103 to be transported to the inside of the conductive member 201a, and then transported to the bottom of the solution box 201b through the electrophoresis rod 201a-1, and at the same time, the current is transported together with the solution at the bottom of the solution box 201b. The liquid is transported to the inside of the detection area 2011 to start the electrophoresis experiment on the capillary. During the experiment, the reaction of the internal sample can be observed in real time through the transparent plate 201f-1. After the electrophoresis experiment is completed, the sample in the detection area 2011 and the solution in the solution tank 201b are transported to the inside of the glass electrophoresis cover 202b through the discharge port 201b-1. The space between the glass electrophoresis cover 202b and the solution tank 201b collects the solution and samples at the end of the experiment, and finally discharges them to a designated location through the waste liquid port 202c to prevent the solution from being contaminated and the inability to uniformly collect and process the tested solution, which leads to inaccurate test results and environmental pollution.

[0041] Compared with Example 2, further, a waste liquid port 202c is provided on one side of the glass electrophoresis cover 202b, and the waste liquid port 202c cooperates with the discharge port 201b-1, and a liquid inlet tank 202d is provided on the top of the glass electrophoresis cover 202b, and the liquid inlet tank 202d is slidably connected to the liquid inlet 201c. When the electrophoresis power supply 102 performs electrophoresis detection on the inside of the detection area 201l, the solution box 201b can be stably fixed on the anti-reflection bottom plate 202a through the connection between the anti-reflection bottom plate 202a and the mounting plate 201b-3. At the same time, the anti-reflection bottom plate 202a supports the bottom of the solution box 201b and the detection area 201l to prevent the reverse outflow of protein in the detection area 201l during the experiment, thereby avoiding experimental failure, improving the efficiency, accuracy and environmental protection of the experiment, and reducing resource waste.

[0042] During use, after the capillary sample is injected into the detection area 201l, it is mixed with the solution inside the solution boxes 201b on both sides. The connection between the electrophoresis connecting line 103 and the conductive member 201a allows the current of the electrophoresis connecting line 103 to be transported to the inside of the conductive member 201a, and then transported to the bottom of the solution box 201b through the electrophoresis rod 201a-1. At the same time, the current is transported to the inside of the detection area 201l together with the solution at the bottom of the solution box 201b to start the electrophoresis experiment on the capillary. During the experiment, the reaction of the internal sample can be observed in real time through the transparent plate 201f-1. After the electrophoresis experiment is completed, the sample inside the detection area 201l and the solution inside the solution box 201b are transported to the inside of the glass electrophoresis cover 202b through the drain port 201b-1. The glass electrophoresis cover 20 The space between 2b and the solution box 201b is used to collect the solutions and samples at the end of the experiment, and finally discharge them to the designated location through the waste liquid port 202c to prevent the solution from being contaminated and the inability to uniformly collect and process the tested solutions, which would lead to inaccurate test results and environmental pollution. At the same time, when the electrophoresis power supply 102 performs electrophoresis detection on the inside of the detection area 201l, the solution box 201b can be stably fixed on the anti-reflection bottom plate 202a through the connection between the anti-reflection bottom plate 202a and the mounting plate 201b-3. At the same time, the anti-reflection bottom plate 202a supports the bottom of the solution box 201b and the detection area 201l to prevent the reverse gushing of proteins in the detection area 201l during the experiment, thereby avoiding experimental failure, improving the efficiency, accuracy and environmental protection of the experiment, and reducing resource waste.

[0043] The remaining structure is the same as that of Example 2.

[0044] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A sample injection device for a capillary isotachophoresis apparatus, characterized in that: include: An electrophoresis unit (100) comprises an electrophoresis operating element (101), an electrophoresis power source (102) arranged inside the electrophoresis operating element (101), and an electrophoresis connection line (103) arranged on the electrophoresis power source (102); The sampling detection unit (200) comprises a sampling component (201) arranged at the other end of the electrophoresis connection line (103), and a recovery component (202) arranged at the bottom of the sampling component (201); The sample injection assembly (201) comprises a conductive member (201a) arranged at the other end of the electrophoresis connecting line (103), a solution box (201b) arranged at the bottom of the conductive member (201a), a liquid inlet (201c) arranged at the top of the solution box (201b), a filter plate (201d) arranged inside the solution box (201b), a rotating shaft (201e) rotatably arranged inside the solution box (201b), and a pressure cover (201f) arranged at the other end of the rotating shaft (201e), and the pressure cover (201f) is rotatably connected to the solution box (201b); An electrophoresis rod (201a-1) is provided at the bottom of the conductive member (201a), and the electrophoresis rod (201a-1) penetrates and is connected to the solution box (201b); A liquid discharge port (201b-1) is provided on one side of the bottom of the solution box (201b), and a liquid guide port (201b-2) is provided on the other side of the bottom of the solution box (201b); A slide groove (201h) is provided on the outer diameter of one side of the solution box (201b), a detection area (201l) is slidably connected inside the slide groove (201h), and one side of the detection area (201l) penetrates and is connected to the liquid guide port (201b-2); An electrophoresis comb (201i) is slidably disposed inside the detection area (201l), and the electrophoresis comb (201i) is slidably connected to the solution box (201b); A limit buckle (201g) is slidably provided on the top of the solution box (201b), and the limit buckle (201g) is slidably connected to the pressure cover (201f); A transparent plate (201f-1) is provided inside the gland (201f), and a mounting plate (201b-3) is provided at the bottom of the solution tank (201b); The recovery component (202) comprises an anti-reflection bottom plate (202a) arranged at the bottom of the solution box (201b), and a glass electrophoresis cover (202b) slidably arranged inside the anti-reflection bottom plate (202a), and the glass electrophoresis cover (202b) is connected to the conductive member (201a); A waste liquid port (202c) is provided on one side of the glass electrophoresis cover (202b), and the waste liquid port (202c) cooperates with the liquid discharge port (201b-1); a liquid inlet groove (202d) is provided on the top of the glass electrophoresis cover (202b), and the liquid inlet groove (202d) is slidably connected to the liquid inlet (201c).

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