Method for synchronously measuring various ammonia and organic amines in soil and sediments

Abstract

The invention discloses a method for synchronously measuring various ammonia and organic amines in soil and sediments, which comprises the following steps: carrying out first extraction operation on the basis of a single extraction reagent, and oscillating and standing a product of the first extraction operation; preparing a to-be-detected solution according to the turbid condition of an oscillation standing operation product; setting optimized chromatographic conditions, and based on the optimized chromatographic conditions, detecting the to-be-detected liquid by adopting a cation chromatographic column and an electrical conductivity detector to obtain the contents of the four kinds of ammonia and organic amines in the soil and the sediments; according to the invention, four kinds of ammonia and organic amines in soil and sediments can be accurately, efficiently and rapidly measured; the pretreatment steps are simple, convenient and rapid, the target compound can be extracted and measured at the same time only through one extracting agent, and no obvious pungent smell exists in the whole process; the method has the advantages of high recovery rate and detection efficiency, small detection interference, reliable detection result and low detection limit, and is suitable for determination of trace ammonia and organic amine in soil and sediments.

Description

Technical Field

[0001] The invention relates to the technical field of environmental detection, and in particular to a method for synchronously determining multiple ammonia and organic amines in soil and sediment. Background Art

[0002] Under standard temperature and pressure conditions, ammonia appears as a colorless gas that emits a strong irritant and foul odor. Its molecular weight is about 17.03, vapor pressure is 506.62 kPa (4.7°C), melting point is -77.7°C, boiling point is -33.5°C, density is 0.771 g / L, and it is easily soluble in water, ethanol, and ether. Organic amines are derivatives of ammonia molecules in which one or more hydrogen atoms are replaced by hydroxyl groups. They can be divided into various types according to the length of the carbon chain, mainly including 34 short-chain organic amines such as methylamine, dimethylamine, trimethylamine, and butylamine, as well as fatty amines with carbon atoms between C8 and C22.

[0003] Ammonia and organic amines, as the second most serious source of odor pollution after organic sulfur compounds, are irritating, toxic and persistent. They not only pose a threat to the natural environment such as soil, but also seriously affect human health. Specifically, ammonia can dissolve human tissue proteins, inhibit enzyme activity and stimulate the central nervous system; methylamine has irritating and corrosive effects on the skin and respiratory tract; dimethylamine is a precursor of nitrosamine carcinogens due to its low olfactory threshold; and trimethylamine not only irritates the eyes, nose and throat, but also inhibits the synthesis of biological macromolecules, with the potential risk of causing embryonic malformations. In view of the increasingly prominent pollution problems of ammonia and organic amine compounds in recent years, it is crucial to accurately, efficiently and quickly identify the types and contents of these compounds in soil and sediments for the maintenance of the ecological environment and human health.

[0004] In the existing technology, there are relatively few studies on ammonia and organic amines, and the main analytical methods include high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, ion selective electrode method, spectrophotometry and ion chromatography, etc. Most of these methods focus on food, workplace, industrial methanol, and ambient air and waste gas, and are guided by a series of relevant national standards; however, in practical applications, these methods have their own limitations, such as:

[0005] (1) HPLC requires cumbersome derivatization steps;

[0006] (2) Gas chromatography and gas chromatography-mass spectrometry are costly, technically demanding, and have poor reproducibility;

[0007] (3) The ion selective electrode method is not sensitive enough; the spectrophotometric method is susceptible to interference and some color developers are toxic;

[0008] (4) In contrast, ion chromatography has the advantages of high response, high sensitivity, good selectivity, low detection limit, high precision, and is environmentally friendly. However, no research has been found on the determination of ammonia and organic amines in soil and sediment using ion chromatography. Summary of the invention

[0009] The object of the present invention is to provide a method for the simultaneous determination of various ammonia and organic amines in soil and sediment, thereby solving all or one of the above problems existing in the prior art.

[0010] In order to solve the above technical problems, the specific technical solutions of the present invention are as follows:

[0011] The present invention provides a method for synchronously determining a variety of ammonia and organic amines in soil and sediment, comprising the following steps:

[0012] Efficient sample pretreatment steps:

[0013] Performing a first extraction operation based on a single extraction reagent, and performing an oscillation and static operation on a product of the first extraction operation; and preparing a test solution according to the turbidity of the product of the oscillation and static operation;

[0014] Determination steps:

[0015] Optimized chromatographic conditions are set, and based on the optimized chromatographic conditions, a cation chromatographic column and a conductivity detector are used to detect the test liquid to obtain the contents of four types of ammonia and organic amines in the soil and sediment.

[0016] In one embodiment, the first extraction operation includes:

[0017] Weighing a first mass of sample and placing it in a first centrifuge tube;

[0018] A second mass and a first concentration of the single extraction reagent are added to the centrifuge tube, and vortex mixed for a first time period.

[0019] In one embodiment, the oscillating static operation includes:

[0020] Using a horizontal oscillator to oscillate the product of the first extraction operation at room temperature for a second period of time at a first low speed;

[0021] After the shaking at room temperature, the mixture is allowed to stand for a third period of time.

[0022] In one embodiment, the step of preparing the test solution according to the turbidity of the product of the oscillation and static operation comprises:

[0023] When the turbidity of the product of the shaking and standing operation is that the supernatant is clear, the clear supernatant of the mixture after standing is taken as the test solution.

[0024] In one embodiment, the step of preparing the test solution according to the turbidity of the product of the oscillation and static operation further comprises:

[0025] When the turbidity of the oscillation and static operation product is that the supernatant is turbid, the test solution is transferred to a second centrifuge tube, and the second centrifuge tube is placed in a centrifuge and centrifuged at a second high speed for a fourth time;

[0026] The supernatant after centrifugation is filtered through a microporous filter membrane of the first parameter, and the filtrate is taken as the test liquid.

[0027] In one embodiment, the optimizing chromatographic conditions comprises:

[0028] Chromatographic column used: Metrosep C4-150 / 4.0;

[0029] The eluent used was: 2,6-pyridinedicarboxylic acid at a concentration of 0.7 mmol / L;

[0030] Flow rate: 0.9 mL / min;

[0031] Injection volume: 20 μL.

[0032] In one embodiment, the determining step further comprises:

[0033] Using pure water as solvent, a mixed standard working solution of four ammonia and organic amines with a concentration of 8.0 μg / mL was prepared;

[0034] 2.5 mL, 5 mL, 10 mL, 25 mL and 50 mL of the mixed standard working solution were respectively measured and transferred to a 100 mL volumetric flask, fixed to volume with pure water and thoroughly mixed to obtain four mixed standard solutions of ammonia and organic amine mixed standard series;

[0035] According to the optimized chromatographic conditions, the mixed standard solutions of the four ammonia and organic amine mixed standard series are measured; the standard curves of the four ammonia and organic amines are drawn with the mass concentration of the target compound as the horizontal coordinate and the chromatographic peak area as the vertical coordinate.

[0036] In one embodiment, the concentrations of the mixed standard solutions of the four mixed standard series of ammonia and organic amines are 0.2 μg / mL, 0.4 μg / mL, 0.8 μg / mL, 2.0 μg / mL and 4.0 μg / mL, respectively.

[0037] In one embodiment, the single extraction reagent is: sulfuric acid solution;

[0038] The first mass is 2.00 g;

[0039] The second mass is 10 mL;

[0040] The first concentration is 0.01 mol / L;

[0041] The first duration is 2 minutes;

[0042] The first low speed is 100r / min;

[0043] The second duration is 30 minutes;

[0044] The third duration is 10 minutes;

[0045] The second highest speed is 3000r / min;

[0046] The fourth duration is 5 minutes;

[0047] The first parameter is 0.45 μm.

[0048] In one embodiment, the four types of ammonia and organic amines include ammonia, methylamine, dimethylamine and trimethylamine.

[0049] The beneficial effects of the technical solution of the present invention are:

[0050] The present invention can realize accurate, efficient and rapid determination of four kinds of ammonia and organic amines in soil and sediments; the pretreatment steps of the present invention are simple and rapid, and only one extractant is needed to simultaneously extract and determine the target compound, and there is no obvious irritating odor in the whole process; the present invention has high recovery rate and detection efficiency, small detection interference, reliable detection results, low detection limit, and is suitable for the determination of trace ammonia and organic amines in soil and sediments; furthermore, the equipment used in the present invention is low in price and easy to operate, and the risk of cross contamination is avoided. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0052] Figure 1 1 is a schematic flow chart of the method for simultaneously determining various ammonia and organic amines in soil and sediment according to Example 1 of the present invention;

[0053] Figure 2 It is an ion chromatogram of four kinds of ammonia and organic amines in the method for simultaneous determination of multiple kinds of ammonia and organic amines in soil and sediment described in Example 1 of the present invention;

[0054] The markings in the accompanying drawings are as follows:

[0055] 1. Ammonia; 2. Methylamine; 3. Dimethylamine; 4. Trimethylamine. DETAILED DESCRIPTION

[0056] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making a clearer and more definite definition of the protection scope of the present invention.

[0057] In the description of the present invention, it should be noted that the embodiments described in the present invention are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention.

[0058] The terms "first", "second", etc. in the specification and claims of this article and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of this article described here can be implemented in an order other than those illustrated or described here. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, device, product or equipment that includes a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or equipment.

[0059] In the description of the present invention, it should be noted that:

[0060] Ammonia is ammonia;

[0061] Organic amines are organic amines;

[0062] Oscillation extraction is oscillation extraction;

[0063] Ion chromatography is ion chromatography.

[0064] 1. In the embodiments of the present invention, the following listed instruments, reagents and conditions are all used as an implementation method, and the implementation method can be adapted and adjusted according to the implementation environment, objectives or conditions of the method in this embodiment.

[0065] 2. In the embodiments of the present invention, the instruments and equipment used are as follows:

[0066] 1. ECO IC ion chromatograph, equipped with conductivity detector.

[0067] 2. HY-4 speed-regulating multi-purpose oscillator.

[0068] 3. TGL-10B high-speed desktop centrifuge.

[0069] 4. Dura 12V ultrapure water machine.

[0070] 3. In the embodiments of the present invention, the reagent materials and their standards used are as follows:

[0071] 1. Sulfuric acid: ρ(H2SO4)=1.84g / mL, purity is excellent grade;

[0072] 2. Nitric acid: ρ(HNO3)=1.42g / mL, purity is excellent grade;

[0073] 3. 2,6-pyridinedicarboxylic acid: the purity is excellent grade;

[0074] 4. Ammonia standard solution: 1000 μg / mL;

[0075] 5. Methylamine and dimethylamine standard solution: 1000 μg / mL;

[0076] 6. Trimethylamine standard solution: 2000 μg / mL;

[0077] 7. The water used in the experiment is ultrapure water, prepared by Dura 12V ultrapure water system.

[0078] In the description of the present invention, it should be noted that the present invention is mainly used for determining four kinds of ammonia and organic amines in soil and sediment by oscillation extraction-ion chromatography.

[0079] This embodiment provides a method for the simultaneous determination of various ammonia and organic amines in soil and sediments. Figure 1 and Figure 2 As shown, the following steps are included:

[0080] S100, efficient sample pre-treatment steps:

[0081] In this step, the sample extraction operation is performed based on a single extraction reagent, and the extract is subjected to secondary extraction according to the turbidity of the extract to finally obtain the test solution, as follows:

[0082] S101. Sample collection: Soil samples shall be collected according to the relevant provisions of HJ / T166, and sediment samples shall be collected according to the standard of GB17378.3; the collected samples shall be properly stored in clean brown ground-mouth glass bottles, and the samples shall be kept in a light-proof, sealed and refrigerated state during transportation;

[0083] Specifically, after sample collection, if analysis cannot be performed immediately, the sample should be placed in a refrigerated environment below 4°C, kept away from light and sealed, with a maximum shelf life of 7 days.

[0084] S102, sample extraction: accurately weigh 2.00g (accurate to 0.01g) of sample and place it in a 50mL centrifuge tube; then, add 10mL of 0.01mol / L sulfuric acid solution thereto and mix evenly using a vortexer for 2 minutes; then, oscillate the mixture at room temperature at a speed of 100r / min using a horizontal oscillator for 30 minutes; then, let the mixture stand for 10 minutes so that the supernatant becomes clear; take the clear supernatant as the test solution.

[0085] S103. Secondary extraction: When the supernatant is still turbid, transfer the extract to another centrifuge tube and centrifuge it in a centrifuge at 3000r / min for 5 minutes; after centrifugation, take the supernatant and filter it through a 0.45μm microporous filter membrane to obtain the test solution for subsequent testing.

[0086] S200, determination steps:

[0087] In this step, a Metrosep C4-150 / 4.0 cation chromatography column is used for separation and exchange, and a conductivity detector is used for detection; a qualitative analysis is performed based on the retention time of the substance, and at the same time, an external standard method is used for quantitative analysis. The specific steps are as follows:

[0088] S201. Chromatographic conditions during determination, including:

[0089] (1) Chromatographic column used: Metrosep C4-150 / 4.0;

[0090] (2) The eluent used was 2,6-pyridinedicarboxylic acid at a concentration of 0.7 mmol / L;

[0091] (3) Flow rate: 0.9 mL / min;

[0092] (4) Injection volume: 20 μL.

[0093] S202, drawing a standard curve, including:

[0094] S2021. Use pure water as solvent to prepare 8.0 μg / mL of 4 kinds of ammonia and organic amine mixed standard working solutions, and store them in a sealed container in a refrigerated environment below 4°C and away from light;

[0095] S2022, respectively measure 2.5mL, 5mL, 10mL, 25mL and 50mL of the above mixed standard working solution, transfer to a 100mL volumetric flask, make up to volume with pure water and mix thoroughly to prepare 4 mixed standard solutions of ammonia and organic amine mixed standard series; the concentrations of the 4 mixed standard solutions of ammonia and organic amine mixed standard series are 0.2μg / mL, 0.4μg / mL, 0.8μg / mL, 2.0μg / mL and 4.0μg / mL, respectively;

[0096] S2023. According to the chromatographic conditions optimized in S201, the standard solutions of the four ammonia and organic amines are measured; the standard curves of the four ammonia and organic amines are drawn with the mass concentration of the target compound as the horizontal axis and the chromatographic peak area as the vertical axis.

[0097] S203, sample measurement, including: establishing the same instrument conditions according to the calibration curve to perform sample measurement.

[0098] It should be noted that in order to prove the technical effect of this method, the detection limit and quantification limit of this method were calculated as follows: According to the provisions of the "Technical Guidelines for the Development of Environmental Monitoring Analytical Methods" (HJ 168-2020), according to the full procedure of sample analysis, the blank spiked samples with a spiked amount of 3 to 5 times the estimated method detection limit were repeated 10 times. The detection limit was calculated according to the formula LD=2.821S, where S represents the standard deviation of the results of ten parallel measurements, and the lower limit of quantification was set to four times the detection limit; the final experimental results showed that the four types of ammonia and organic amines showed good linear relationships in the concentration range of 0.2-4.0 μg / mL, and the correlation coefficients r were all higher than 0.999. Under the conditions of 2g sample and 10mL fixed volume, the detection limits of these four ammonia and organic amine methods are 0.4mg / kg~1.0mg / kg, and the quantification limits are 1.6mg / kg~4.0mg / kg, which meet the detection requirements of ammonia and organic amines in soil and sediment. The relevant result data table is as follows:

[0099] Table 1, method detection limit and quantification limit of 4 kinds of ammonia and organic amines:

[0100]

[0101] Table 2, standard curve equations and correlation coefficients of 4 kinds of ammonia and organic amines:

[0102]

[0103]

[0104] It should be noted that in order to prove the technical effect of this method, the precision and accuracy of this method were verified: two different types of samples, namely soil samples and sediment samples, were collected in a certain area of ​​Suzhou City, Jiangsu Province; 18 samples of each type were collected, each weighing 2 grams; for these two types of actual samples, three groups of different types of parallel spike recovery tests were carried out, and the spike amounts were set to 3.00μg, 10.0μg and 30.0μg respectively; then, according to the chromatographic strips that have been optimized in this method, The samples were measured, and the spiked recoveries and relative standard deviations (RSDs) of the measurement results were calculated. The results are as follows: for sediment samples, at low, medium and high spiked concentration levels, the spiked recoveries of the four ammonia and organic amines were 82.1% to 98.6%, and the RSDs were 3.9% to 9.0%; for soil samples, also at low, medium and high spiked concentration levels, the spiked recoveries of the four ammonia and organic amines were 85.7% to 101%, and the RSDs were 1.8% to 8.8%. The relevant result data table is as follows:

[0105] Table 3, actual recovery rates and relative standard deviations of four kinds of ammonia and organic amines in soil and sediments:

[0106]

[0107]

[0108] It should be noted that the above examples are only for explaining the present invention and cannot limit the protection scope of the present invention.

[0109] Different from the prior art, the method for synchronous determination of multiple ammonia and organic amines in soil and sediments of the present application can accurately, efficiently and quickly determine four types of ammonia and organic amines in soil and sediments; the pretreatment steps of the present invention are simple and quick, and only one extractant is needed to simultaneously extract and determine the target compounds, and there is no obvious irritating odor throughout the process; the present invention has a high recovery rate and detection efficiency, small detection interference, reliable detection results, and a low detection limit, and is suitable for the determination of trace ammonia and organic amines in soil and sediments; furthermore, the equipment used in the present invention is low in price and easy to operate, avoiding the risk of cross contamination.

[0110] It should be understood that in the various embodiments of this document, the size of the serial numbers of the above-mentioned processes does not mean the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this document.

[0111] It should also be understood that in the embodiments of this article, the term "and / or" is only a description of the association relationship of the associated objects, indicating that three relationships may exist. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0112] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, computer software, or a combination of the two. In order to clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in terms of function in the above description. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this article.

[0113] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working processes of the systems, devices and units described above can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0114] In the several embodiments provided herein, it should be understood that the disclosed systems, devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic, for example, the division of the units is only a logical function division, and there may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the mutual coupling or direct coupling or communication connection shown or discussed can be an indirect coupling or communication connection through some interfaces, devices or units, or can be electrical, mechanical or other forms of connection.

[0115] The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the embodiments of this article.

[0116] In addition, each functional unit in each embodiment of this invention may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above integrated unit may be implemented in the form of hardware or in the form of software functional unit.

[0117] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this article is essentially or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including a number of instructions to enable a computer device (which can be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method described in each embodiment of this article. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk and other media that can store program codes.

[0118] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for simultaneous determination of various ammonia and organic amines in soil and sediment, characterized in that: The following steps are involved: Efficient sample pretreatment steps: Performing a first extraction operation based on a single extraction reagent, and performing an oscillation and static operation on a product of the first extraction operation; and preparing a test solution according to the turbidity of the product of the oscillation and static operation; Determination steps: Optimized chromatographic conditions are set, and based on the optimized chromatographic conditions, a cation chromatographic column and a conductivity detector are used to detect the test liquid to obtain the contents of four types of ammonia and organic amines in the soil and sediment.

2. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 1, characterized in that: The first extraction operation comprises: Weighing a first mass of sample and placing it in a first centrifuge tube; A second mass and a first concentration of the single extraction reagent are added to the centrifuge tube, and vortex mixed for a first time period.

3. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 2, characterized in that: The oscillating static operation comprises: Using a horizontal oscillator to oscillate the product of the first extraction operation at room temperature for a second period of time at a first low speed; After the shaking at room temperature, the mixture is allowed to stand for a third period of time.

4. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 3, characterized in that: The step of preparing the test solution according to the turbidity of the product of the oscillation and static operation comprises: When the turbidity of the product of the shaking and standing operation is that the supernatant is clear, the clear supernatant of the mixture after standing is taken as the test solution.

5. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 4, characterized in that: The step of preparing the test solution according to the turbidity of the product of the oscillation and static operation further comprises: When the turbidity of the oscillation and static operation product is that the supernatant is turbid, the test solution is transferred to a second centrifuge tube, and the second centrifuge tube is placed in a centrifuge and centrifuged at a second high speed for a fourth time; The supernatant after centrifugation is filtered through a microporous filter membrane of the first parameter, and the filtrate is taken as the test liquid.

6. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 1, characterized in that: The optimized chromatographic conditions include: Chromatographic column used: MetrosepC4-150 / 4.0; The eluent used was: 2,6-pyridinedicarboxylic acid at a concentration of 0.7 mmol / L; Flow rate: 0.9 mL / min; Injection volume: 20 μL.

7. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 1, characterized in that: The measuring step further comprises: Using pure water as solvent, a mixed standard working solution of four ammonia and organic amines with a concentration of 8.0 μg / mL was prepared; 2.5 mL, 5 mL, 10 mL, 25 mL and 50 mL of the mixed standard working solution were respectively measured and transferred to a 100 mL volumetric flask, fixed to volume with pure water and thoroughly mixed to obtain four mixed standard solutions of ammonia and organic amine mixed standard series; According to the optimized chromatographic conditions, the mixed standard solutions of the four ammonia and organic amine mixed standard series are measured; the standard curves of the four ammonia and organic amines are drawn with the mass concentration of the target compound as the horizontal coordinate and the chromatographic peak area as the vertical coordinate.

8. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 7, characterized in that: The concentrations of the mixed standard solutions of the four ammonia and organic amine mixed standard series are 0.2 μg / mL, 0.4 μg / mL, 0.8 μg / mL, 2.0 μg / mL and 4.0 μg / mL, respectively.

9. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 5, characterized in that: The single extraction reagent is: sulfuric acid solution; The first mass is 2.00 g; The second mass is 10 mL; The first concentration is 0.01 mol / L; The first duration is 2 minutes; The first low speed is 100r / min; The second duration is 30 minutes; The third duration is 10 minutes; The second highest speed is 3000r / min; The fourth duration is 5 minutes; The first parameter is 0.45 μm.

10. The method for simultaneous determination of various ammonia and organic amines in soil and sediment according to claim 7, characterized in that: The four types of ammonia and organic amines include ammonia, methylamine, dimethylamine and trimethylamine.

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