Cultivation method of head smut-resistant glutinous sorghum restorer line

Abstract

The invention relates to the technical field of agriculture, and discloses a head smut-resistant glutinous sorghum restorer line cultivation method which comprises the following steps: S1, selecting full-grain early-maturing dwarf dense-tolerant sterile line seeds as a first female parent, selecting full-grain glutinous sorghum seeds as a first male parent, hybridizing the first female parent and the first male parent to obtain a primary generation seed, and breeding the primary generation seed into a second female parent; continuously selfing the primary seeds for six generations; and S2, hybridizing by taking the sterile line of the japonica sorghum as a female parent and the bred primary-generation seed as a male parent to obtain a second-generation seed named FB357. According to the cultivation method of the head smut-resistant glutinous sorghum restorer line, the restorer line sorghum seeds cultivated through the above steps can accurately eliminate head smut-resistant strains through an indoor identification method and a field identification method, the breeding efficiency is improved, the morbidity is low in the planting process, and the yield is high. The situation of large-scale occurrence of head smut in a sorghum field in production is avoided, the purpose of increasing production and income is achieved, and the sorghum yield is effectively increased through multiple hybridization and selfing.

Description

Technical Field

[0001] The invention relates to the technical field of agriculture, and in particular to a method for cultivating a head smut-resistant glutinous sorghum restorer line. Background Art

[0002] Head smut is one of the main diseases affecting the development of sorghum production in my country. It occurs every year in sorghum producing areas, with the most serious damage in Northeast China and North China. The incidence rate is sometimes as high as 70%, causing great losses to production. Production practice has shown that the use of pesticides or cultivation measures for prevention and control has limitations such as being difficult to implement, not economical, having unsatisfactory prevention and control effects, and causing environmental pollution. Domestic and foreign studies have shown that the main measure to prevent and control the disease is to plant disease-resistant varieties. It is necessary to invent a breeding method for sorghum resistant to head smut in order to breed sorghum varieties resistant to head smut. Currently, most disease resistance tests are conducted in the field, and the identification efficiency is slow. Summary of the invention

[0003] 1. Technical issues to be resolved

[0004] In view of the shortcomings of the prior art, the present invention provides a method for cultivating a glutinous sorghum restorer line resistant to head smut, which solves the problem that most of the current disease resistance tests are conducted in the field and the identification efficiency is slow.

[0005] (II) Technical solution

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method for breeding a glutinous sorghum restorer line resistant to head smut, comprising the following steps:

[0008] S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for six consecutive generations;

[0009] S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for six consecutive generations;

[0010] S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for six consecutive generations to obtain restorer planting materials;

[0011] S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals;

[0012] S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears;

[0013] S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being between 70%, amylopectin accounting for more than 90% of the total starch, and the endosperm reacting red with iodine;

[0014] S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3;

[0015] S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination;

[0016] S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%;

[0017] S6B), indoor growth cone identification includes steps A, B and C;

[0018] S6C), using disease-resistant strains in both selfing and hybridization.

[0019] Preferably, the specific steps are as follows: Step A, indoor growth cone identification: taking the head smut resistance strains of the seeds obtained in S6A as units, taking 100 seeds from each ear, and performing resistance identification indoors;

[0020] Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain;

[0021] Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

[0022] Preferably, said S1, S2 and S3 are self-pollinated continuously for at least six times.

[0023] Preferably, the natural pollination process in S5 can be pollinated according to the bagging method.

[0024] Preferably, in the SN020 seed production process, the female parent's growth period is 110 days, and the male parent's growth period is 115 days.

[0025] Preferably, the dyeing formula of the aniline blue lactic acid dye in step B is: 0.1-0.2 g of aniline blue, 50-60 ml of lactic acid, and 100 ml of water.

[0026] (III) Beneficial effects

[0027] Compared with the prior art, the present invention provides a method for breeding a waxy sorghum restorer line resistant to head smut, which has the following beneficial effects:

[0028] 1. A method for breeding a restorer line of glutinous sorghum resistant to head smut. The restorer line sorghum seeds bred through the above steps can accurately eliminate strains that are not resistant to head smut through indoor identification methods and field identification methods, thereby improving breeding efficiency. The disease incidence rate is low during the planting process, thereby avoiding the large-scale occurrence of head smut in sorghum fields during production, achieving the purpose of increasing production and income, and effectively increasing the yield of the sorghum through multiple hybridization and selfing.

[0029] 2. The method for breeding the glutinous sorghum restoration line resistant to head smut is to hybridize and self-pollinate through the above steps, thereby significantly increasing the yield of the sorghum, and having strong lodging resistance, good herbicide resistance and high survival rate. DETAILED DESCRIPTION

[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0031] Embodiment 1:

[0032] A method for breeding a glutinous sorghum restorer line resistant to head smut, comprising the following steps:

[0033] S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for six consecutive generations;

[0034] S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for six consecutive generations;

[0035] S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for six consecutive generations to obtain restorer planting materials;

[0036] S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals;

[0037] S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears;

[0038] S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being 70%, amylopectin accounting for more than 90% of the total starch, and the endosperm reacting red with iodine;

[0039] S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3;

[0040] S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination;

[0041] S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%;

[0042] S6B), indoor growth cone identification includes steps A, B and C;

[0043] S6C), using disease-resistant strains in both selfing and hybridization;

[0044] The specific steps are as follows: Step A, indoor growth cone identification: Taking the head smut-resistant strains of seeds obtained in S6A as units, 100 seeds were taken from each ear and resistance identification was performed indoors;

[0045] Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain;

[0046] The dyeing formula of aniline blue lactic acid dye is: aniline blue 0.1g, lactic acid 50ml, water 100ml;

[0047] Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

[0048] The restorer sorghum varieties cultivated through the above steps can accurately eliminate strains that are not resistant to head smut through indoor identification and field identification methods, thereby improving breeding efficiency, and having a low incidence rate during the planting process, thereby avoiding the large-scale occurrence of head smut in sorghum fields during production, achieving the goal of increasing production and income, and effectively increasing the yield of the sorghum through multiple hybridization and self-pollination.

[0049] Embodiment 2:

[0050] A method for breeding a glutinous sorghum restorer line resistant to head smut, comprising the following steps:

[0051] S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for seven consecutive generations;

[0052] S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for seven consecutive generations;

[0053] S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for seven consecutive generations to obtain restorer planting materials;

[0054] S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals;

[0055] S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears;

[0056] S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being 70%, amylopectin accounting for 90% of the total starch, and the endosperm reacting red with iodine;

[0057] S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3;

[0058] S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination;

[0059] S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%;

[0060] S6B), indoor growth cone identification includes steps A, B and C;

[0061] S6C), using disease-resistant strains in both selfing and hybridization;

[0062] The specific steps are as follows: Step A, indoor growth cone identification: Taking the head smut-resistant strains of seeds obtained in S6A as units, 100 seeds were taken from each ear and resistance identification was performed indoors;

[0063] Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain;

[0064] The dyeing formula of aniline blue lactic acid dye is: aniline blue 0.15g, lactic acid 55ml, water 100ml;

[0065] Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

[0066] The restorer sorghum varieties cultivated through the above steps can accurately eliminate strains that are not resistant to head smut through indoor identification and field identification methods, thereby improving breeding efficiency, and having a low incidence rate during the planting process, thereby avoiding the large-scale occurrence of head smut in sorghum fields during production, achieving the goal of increasing production and income, and effectively increasing the yield of the sorghum through multiple hybridization and self-pollination.

[0067] Embodiment three:

[0068] A method for breeding a glutinous sorghum restorer line resistant to head smut, comprising the following steps:

[0069] S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for six consecutive generations;

[0070] S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for seven consecutive generations;

[0071] S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for eight consecutive generations to obtain restorer planting materials;

[0072] S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals;

[0073] S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears;

[0074] S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being 70%, amylopectin accounting for 90% of the total starch, and the endosperm reacting red with iodine;

[0075] S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3;

[0076] S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination;

[0077] S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%;

[0078] S6B), indoor growth cone identification includes steps A, B and C;

[0079] S6C), using disease-resistant strains in both selfing and hybridization;

[0080] The specific steps are as follows: Step A, indoor growth cone identification: Taking the head smut-resistant strains of seeds obtained in S6A as units, 100 seeds were taken from each ear and resistance identification was performed indoors;

[0081] Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain;

[0082] The dyeing formula of aniline blue lactic acid dye is: aniline blue 0.2g, lactic acid 60ml, water 100ml;

[0083] Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

[0084] The restorer sorghum varieties cultivated through the above steps can accurately eliminate strains that are not resistant to head smut through indoor identification and field identification methods, thereby improving breeding efficiency, and having a low incidence rate during the planting process, thereby avoiding the large-scale occurrence of head smut in sorghum fields during production, achieving the goal of increasing production and income, and effectively increasing the yield of the sorghum through multiple hybridization and self-pollination.

[0085] Embodiment 4:

[0086] A method for breeding a glutinous sorghum restorer line resistant to head smut, comprising the following steps:

[0087] S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for seven consecutive generations;

[0088] S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for seven consecutive generations;

[0089] S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for eight consecutive generations to obtain restorer planting materials;

[0090] S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals;

[0091] S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears;

[0092] S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being 70%, amylopectin accounting for 90% of the total starch, and the endosperm reacting red with iodine;

[0093] S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3;

[0094] S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination;

[0095] S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%;

[0096] S6B), indoor growth cone identification includes steps A, B and C;

[0097] S6C), using disease-resistant strains in both selfing and hybridization;

[0098] The specific steps are as follows: Step A, indoor growth cone identification: Taking the head smut-resistant strains of seeds obtained in S6A as units, 100 seeds were taken from each ear and resistance identification was performed indoors;

[0099] Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain;

[0100] The dyeing formula of aniline blue lactic acid dye is: aniline blue 0.1g, lactic acid 60ml, water 100ml;

[0101] Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

[0102] The restorer sorghum varieties cultivated through the above steps can accurately eliminate strains that are not resistant to head smut through indoor identification and field identification methods, thereby improving breeding efficiency, and having a low incidence rate during the planting process, thereby avoiding the large-scale occurrence of head smut in sorghum fields during production, achieving the goal of increasing production and income, and effectively increasing the yield of the sorghum through multiple hybridization and self-pollination.

[0103] Embodiment five:

[0104] A method for breeding a glutinous sorghum restorer line resistant to head smut, comprising the following steps:

[0105] S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for eight consecutive generations;

[0106] S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for six consecutive generations;

[0107] S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for eight consecutive generations to obtain restorer planting materials;

[0108] S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals;

[0109] S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears;

[0110] S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being 70%, amylopectin accounting for 90% of the total starch, and the endosperm reacting red with iodine;

[0111] S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3;

[0112] S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination;

[0113] S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%;

[0114] S6B), indoor growth cone identification includes steps A, B and C;

[0115] S6C), using disease-resistant strains in both selfing and hybridization;

[0116] The specific steps are as follows: Step A, indoor growth cone identification: Taking the head smut-resistant strains of seeds obtained in S6A as units, 100 seeds were taken from each ear and resistance identification was performed indoors;

[0117] Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain;

[0118] The dyeing formula of aniline blue lactic acid dye is: aniline blue 0.17g, lactic acid 58ml, water 100ml;

[0119] Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

[0120] The restorer sorghum varieties cultivated through the above steps can accurately eliminate strains that are not resistant to head smut through indoor identification and field identification methods, thereby improving breeding efficiency, and having a low incidence rate during the planting process, thereby avoiding the large-scale occurrence of head smut in sorghum fields during production, achieving the goal of increasing production and income, and effectively increasing the yield of the sorghum through multiple hybridization and self-pollination.

[0121] Data comparison and analysis:

[0122] In the variety experiment, the seedling raising method of the present invention has a higher resistance to black stigma, and the yield is obviously higher than that of the comparison variety, and the resistance to diseases and insect pests is strong;

[0123] The following table shows the specific experimental data:

[0124]

[0125] It can be seen from the above table that the restorer sorghum varieties cultivated by the present invention can accurately eliminate strains that are not resistant to head smut through indoor identification methods and field identification methods, thereby improving the efficiency of breeding, and have a low incidence rate during the planting process, thus avoiding the large-scale occurrence of head smut in sorghum fields during production.

[0126] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for breeding a glutinous sorghum restorer line resistant to head smut, characterized in that: The following steps are involved: S1, selecting early-maturing dwarf-dense-tolerant sterile line seeds with full grains as the first female parent, selecting waxy sorghum seeds with full grains as the first male parent, hybridizing the first female parent with the first male parent to obtain the first generation seeds, and the first generation seeds are self-pollinated for six consecutive generations; S2, using the male sterile line of japonica sorghum as the female parent and the first generation seed as the male parent, the second generation seed was obtained and named FB357. FB357 was self-pollinated for six consecutive generations; S3, select restorer japonica sorghum seeds as the third male parent, and use the bred FB357 as the female parent for hybridization to obtain the third generation seed, which is named SN020. SN020 is self-pollinated for six consecutive generations to obtain restorer planting materials; S4, during the continuous selfing process of S1 / S2 / S3, plants showing corresponding characteristics were selected in each generation according to the breeding goals; S4A), the breeding goal is to have strong plants, low height, disease resistance, small ears, and waxy ears; S4B), the waxy property in the breeding goal refers to the starch content of waxy sorghum being between 70%, amylopectin accounting for more than 90% of the total starch, and the endosperm reacting red with iodine; S5, artificial pollination or natural pollination can be selected during the self-pollination and hybridization of S1 / S2 / S3; S6: In S1 / S2 / S3, field disease resistance identification is carried out, disease-resistant plants are selected for cultivation, and seeds are harvested before hybridization or self-pollination; S6A), identification of silk sorghum resistant lines was performed by combining indoor growth cone identification and field identification to identify the F4 generation twice, eliminating lines with high disease incidence and selecting lines with disease incidence below 10%; S6B), indoor growth cone identification includes steps A, B and C; S6C), using disease-resistant strains in both selfing and hybridization.

2. The method for breeding a head smut-resistant waxy sorghum restorer line according to claim 1, characterized in that: The specific steps are as follows: Step A, indoor growth cone identification: taking the head smut resistance strain of the seeds obtained in S6A as a unit, 100 seeds are taken from each ear, and resistance identification is performed indoors; Step B, inoculating the culture box with 0.6% (mass percentage) of bacterial soil, and 25 days after emergence, taking the seedlings, peeling off the outer leaves, leaving the growth cone, and staining with aniline blue lactic acid stain; Step C, microscopically inspecting the mesenchyme at the base of the growth cone of the seedlings for dark blue mycelium, and selecting strains with a seedling disease incidence rate of less than 8%, which are disease-resistant strains.

3. The method for breeding a head smut-resistant waxy sorghum restorer line according to claim 1, characterized in that: The S1, S2 and S3 are self-pollinated continuously for at least six times.

4. The method for breeding a head smut-resistant waxy sorghum restorer line according to claim 1, characterized in that: In the natural pollination process in step S5, pollination is performed according to the bagging method.

5. The method for breeding a head smut-resistant waxy sorghum restorer line according to claim 1, characterized in that: In the SN020 seed production process, the female parent's growth period is 110 days, and the male parent's growth period is 115 days.

6. The method for breeding a restoring line of glutinous sorghum resistant to head smut according to claim 2, characterized in that: The dyeing formula of the aniline blue lactic acid dye in step B is: 0.1-0.2 g of aniline blue, 50-60 ml of lactic acid, and 100 ml of water.

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