Anti-wrinkle and anti-aging composition containing composite peptide and application of anti-wrinkle and anti-aging composition

Abstract

The invention discloses an anti-wrinkle and anti-aging composition containing composite peptide and application of the anti-wrinkle and anti-aging composition, and aims to provide a composition which can repair skin barriers, supplement collagen and elastic fibers, improve skin elasticity, reduce skin wrinkles and delay skin aging and has anti-aging, anti-wrinkle and moisturizing effects. According to the technical scheme, the compound peptide mainly comprises compound peptide, wherein the compound peptide comprises arginine / lysine polypeptide, acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, acetyl tetrapeptide-9 and acetyl tetrapeptide-11; belongs to the technical field of cosmetics.

Description

Technical Field

[0001] The invention discloses an anti-wrinkle and anti-aging composition, specifically, an anti-wrinkle and anti-aging composition containing a composite peptide. The invention also discloses the application of the anti-wrinkle and anti-aging composition containing the composite peptide as a skin care product additive; and belongs to the technical field of cosmetics. Background Art

[0002] As we age, the structure of our skin changes, which is mainly reflected in the following aspects: (1) The epidermis becomes thinner and the cell renewal rate slows down, causing the skin to lose elasticity. (2) The content of collagen fibers and elastic fibers in the dermis decreases, causing the skin to sag. (3) The fat in the subcutaneous tissue decreases and the skin loses support. In addition, the moisture content in the skin will gradually decrease, which will cause the skin to become dry and lose its original elasticity and firmness. Over time, fine lines and wrinkles will appear on the surface of the skin, showing obvious signs of aging. Therefore, in the process of daily skin care, anti-aging and anti-wrinkle have always been the focus of people's skin care. By using various anti-wrinkle and anti-aging skin care products, the moisture and nutrients needed by the skin can be replenished, the aging process of the skin can be delayed, and the skin can be kept healthy and youthful.

[0003] Therefore, the research and development of anti-wrinkle and anti-aging products has also become a research focus for researchers. For example, CN118593400A provides an anti-wrinkle and anti-aging composition containing Chinese medicinal ingredients and its preparation method and application, using Bidens pilosa extract, snow tea leaf extract, Rosa roxburghii fruit extract, Phyllanthus emblica fruit extract, Viola extract, Tribulus terrestris fruit extract, Scrophularia ningpoensis extract and Bakuchiol. Chinese patent CN118001205A provides an anti-wrinkle active composition and its application, anti-wrinkle cream, which is composed of 1-6% recombinant type III collagen, 30-45% alpine edelweiss extract, 5-20% ginsenoside CK and 30-45% acetyl hexapeptide-8. The four active ingredients cooperate in a variety of ways, so that the anti-wrinkle active composition can effectively prevent and reduce wrinkles on facial skin, scalp skin and neck skin, help restore skin elasticity, enhance collagen strength, enhance skin barrier function and hydration and moisturizing effects.

[0004] Although the above products have certain anti-wrinkle and anti-aging effects, either the amount of active ingredients added is high and the price is expensive, or the concept is added, the anti-wrinkle and anti-aging effects are not ideal and cannot meet the needs of customers. Summary of the invention

[0005] In view of the above problems, the purpose of the present invention is to provide an anti-aging, anti-wrinkle and moisturizing composition that can repair the skin barrier, replenish the skin's moisturizing ability, replenish collagen and elastic fibers, improve skin elasticity, reduce skin wrinkles, and delay skin aging.

[0006] The second technical solution provided by the present invention is the use of the anti-wrinkle and anti-aging composition containing the composite peptide in the first technical solution as a cosmetic additive.

[0007] To this end, the first technical solution provided by the present invention is as follows:

[0008] The invention discloses an anti-wrinkle and anti-aging composition containing a composite peptide, which mainly comprises the composite peptide, wherein the composite peptide comprises arginine / lysine polypeptide, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, acetyl tetrapeptide-9 and acetyl tetrapeptide-11.

[0009] Furthermore, in the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide, the composite peptide also includes a moisturizer and a solvent.

[0010] Furthermore, in the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide, the moisturizer includes any one of sorbitol, polyethylene glycol, hexylene glycol, betaine, glycerin, propylene glycol, butylene glycol, methylpropanediol or pentylene glycol.

[0011] Furthermore, the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide, the composite peptide is composed of the following components in percentage by mass: acetyl tetrapeptide-11 0.01% to 1%; acetyl tetrapeptide-9 0.01% to 1%; dipeptide diaminobutyryl benzylamide diacetate 0.01% to 1%; acetyl hexapeptide-8 0.01% to 1%; arginine / lysine polypeptide 0.001% to 1%; butanediol 20% to 50%, and the balance water.

[0012] Furthermore, the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide, the composite peptide is composed of the following components in percentage by mass: acetyl tetrapeptide-110.03%; acetyl tetrapeptide-90.03%; dipeptide diaminobutyryl benzylamide diacetate 0.05%; acetyl hexapeptide-80.05%; arginine / lysine polypeptide 0.003%; butanediol 40%, and the balance water.

[0013] Furthermore, the above anti-wrinkle and anti-aging composition containing a composite peptide further comprises 0.3-0.8% of banana fruit extract.

[0014] Furthermore, the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide also includes 0.25-0.5% of Tremella fuciformis fruiting body extract and 0.3-0.8% of oregano flower oil.

[0015] Furthermore, the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide also includes 0.1-0.3% of Guangxi Mandarin orange peel extract, 0.1-0.3% of Rosa canina fruit oil and 0.1-0.5% of Nigella nigra seed oil.

[0016] Furthermore, in the above-mentioned anti-wrinkle and anti-aging composition containing a composite peptide, the banana fruit extract is prepared by the following method:

[0017] S1: peel fresh banana fruit and place it in an extraction container, then add deionized water in an amount of 1-2 times the weight of the banana fruit, and crush it to obtain the extract;

[0018] S2: adding antioxidant to the extract, heating to 45-55°C, soaking for 6-8 hours to obtain a crude liquid;

[0019] S3: Add 0.5-1.5wt% sodium chloride, 0.8-1.2wt% sodium dihydrogen phosphate, and 2-2.5wt% sucrose to the crude extract and mix well. 7 CFU / mL inoculation volume: 1×10 6 Inoculate Lactobacillus bulgaricus at an inoculum amount of 100 CFU / mL and Bacillus subtilis at an inoculum amount of 1×107 CFU / mL, ferment at 32-36° C. for 7-14 days, sterilize, and obtain a fermentation product;

[0020] S4: adding chitosan to the fermented product and stirring for 1-3 hours, filtering through a 200-mesh sieve to obtain a primary filtrate, and then filtering through a 100-150 μm microporous filter membrane to obtain a refined filtrate; the refined filtrate is concentrated under reduced pressure to 1 / 3-1 / 4 of the primary filtrate, and freeze-dried to obtain the banana fruit extract.

[0021] The second technical solution provided by the present invention is the application of the anti-wrinkle and anti-aging composition containing the composite peptide as a cosmetic additive, and the added amount accounts for 1-10% of the total amount of the cosmetics.

[0022] Compared with the prior art, the technical solution provided by the present invention has the following technical advantages:

[0023] 1. The composition provided by the present application can effectively promote the regeneration and repair of skin cells, activate skin cells, and revitalize them, thereby achieving an anti-aging effect. At the same time, it can also promote the renewal of skin surface cells, making the skin smoother and more delicate, and having an anti-wrinkle effect.

[0024] 2. The composition provided in the present application has a good moisturizing effect, penetrates deep into the bottom layer of the skin, provides lasting moisture to the skin, maintains the moisture balance of the skin, thereby making the skin have sufficient elasticity and firmness, thereby reducing the formation of wrinkles.

[0025] 3. The polypeptide factor provided in this application can also repair the barrier function of the skin, replenish collagen, promote the proliferation of human skin fibroblasts and the synthesis of extracellular matrix, thereby improving the firmness and elasticity of the skin.

[0026] 4. The composition provided in the present application is easy to absorb. The molecular structure of the polypeptide factor is small and can be quickly absorbed by the skin, exerting anti-aging, anti-wrinkle and moisturizing effects. It has good compatibility and can be used in combination with various matrices to enhance the skin care effect.

[0027] 5. The technical solution provided by the present invention also includes a formula of Tremella fuciformis fruiting body extract, banana fruit extract, oregano flower oil, Guangxi mandarin orange peel extract, Rosa canina fruit oil and Nigella nigra seed oil, which are rich in polysaccharides, terpenoids, fatty acids, amino acids, vitamins and other nutrients, which further synergize with the composite peptide component of the present application to promote the proliferation and differentiation of skin cells, promote the repair and regeneration of skin cells, improve skin elasticity, reduce wrinkles, relieve skin inflammation, remove free radicals in the skin, and prevent free radicals from damaging skin cells, thereby further improving the skin's immunity, resisting the invasion of the external environment on the skin, and delaying skin aging.

[0028] 6. The technical solution provided by the present invention adopts a combination of water extraction, fermentation extraction and the like to extract banana fruit, thereby further improving the activity and bioavailability of the extract and increasing the content of active ingredients in the banana fruit extract. DETAILED DESCRIPTION

[0029] The present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that, if no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0030] Before describing the embodiments in detail, necessary descriptions are first made on the raw materials involved in each embodiment and the method for preparing fermented oil in each embodiment. Unless otherwise specified, each embodiment is prepared according to this method:

[0031] The raw materials involved in the embodiment are shown in Table 1:

[0032] Table 1

[0033] raw material supplier Butanediol Shanghai Qiao Chemical Technology Co., Ltd. Arginine / Lysine Peptide Jining Huanju Pharmaceutical Technology Co., Ltd. Acetyl Hexapeptide-8 Jining Huanju Pharmaceutical Technology Co., Ltd. Dipeptide diaminobutyryl benzylamide diacetate Jining Huanju Pharmaceutical Technology Co., Ltd. Acetyl Tetrapeptide-9 Jining Huanju Pharmaceutical Technology Co., Ltd. Acetyl Tetrapeptide-11 Jining Huanju Pharmaceutical Technology Co., Ltd. Tremella fuciformis fruiting body extract Shandong Fengtai Biotechnology Co., Ltd. Winter oregano flower oil Beijing Lingbao Technology Co., Ltd. Guangxi Citrus aurantium peel extract Weiyi Peptide (Guangdong) Biotechnology Co., Ltd. Rosa canina fruit oil Wuhan Xinxinjiali Biotechnology Co., Ltd. Black tea seed oil Shaanxi Didu Pharmaceutical Chemical Co., Ltd. Plantain Fruit Extract self made

[0034] The plantain fruit extract described in the following examples was prepared by the following method:

[0035] S1: peel fresh banana fruit, weigh them, sterilize them with ultraviolet light, put them in an extraction container, add deionized water with the weight of 2 banana fruit into the container, and crush them to obtain the extract;

[0036] S2: adding 1 wt% antioxidant to the extract (, heating to 55°C, soaking for 7 hours, to obtain a crude liquid;

[0037] S3: Add 1 wt% sodium chloride, 1 wt% sodium dihydrogen phosphate, and 2.5 wt% sucrose to the crude extract and mix well. 7 CFU / mL inoculation volume: 1×10 6 Inoculate Lactobacillus bulgaricus at an inoculum amount of 100 CFU / mL and Bacillus subtilis at an inoculum amount of 1×107 CFU / mL, ferment at 32-36°C for 10 days, sterilize at 121°C for 12 min, and obtain a fermentation product;

[0038] S4: adding chitosan (10 wt% by weight of the fermentation product) to the fermentation product and stirring for 2 hours, filtering through a 200-mesh sieve to obtain a primary filtrate, and then filtering through a 150 μm microporous filter membrane to obtain a refined filtrate; the refined filtrate is concentrated under reduced pressure at 0.09 MPa and 60° C. to 1 / 3-1 / 4 of the primary filtrate, and then freeze-dried at -60° C. for 24 hours to obtain the banana fruit extract.

[0039] The components and their mass percentages involved in Examples 1-5 of the present application are shown in Table 2.

[0040]

[0041] The components and their mass percentages involved in Examples 6-8 of the present application are shown in Table 3.

[0042] Table 3

[0043] Components Example 6 Example 7 Example 8 Butanediol 40.000％ 40.000％ 40.000％ Arginine / Lysine Peptide 0.003％ 0.003％ 0.003％ Acetyl Hexapeptide-8 0.05％ 0.05％ 0.05％ Dipeptide diaminobutyryl benzylamide diacetate 0.05％ 0.05％ 0.05％ Acetyl Tetrapeptide-9 0.030％ 0.030％ 0.030％ Acetyl Tetrapeptide-11 0.030％ 0.030％ 0.030％ Plantain Fruit Extract 0.300％ 0.500％ 0.800％ water 59.5370％ 59.3370％ 59.0370％

[0044] The components and their mass percentages involved in implementations 9-11 of this application are shown in Table 4.

[0045] Table 4

[0046]

[0047]

[0048] The various components and their mass percentages involved in implementations 12-14 of the present application are shown in Table 5.

[0049] Table 5

[0050]

[0051]

[0052] In order to better demonstrate the development process and effect of the composition provided in this application, the comparative examples in the development process of this application are given below:

[0053] Comparative Example 1

[0054]

[0055]

[0056] It should be noted that:

[0057] The sources of the components in Comparative Examples 1-5 are the same as those in the examples. The preparation method of the banana fruit extract in Comparative Example 6 is as follows:

[0058] S1: peel fresh banana fruit, weigh them, sterilize them with ultraviolet light, put them in an extraction container, add deionized water with the weight of 2 banana fruit into the container, and crush them to obtain the extract;

[0059] S2: adding 1 wt% antioxidant to the extract and heating to 55°C, soaking for 7 hours to obtain a crude liquid;

[0060] S3: adding chitosan (10 wt% by weight of the crude liquid) to the crude liquid and stirring for 2 hours, filtering through a 200-mesh sieve to obtain a primary filtrate, and then filtering through a 150 μm microporous filter membrane to obtain a refined filtrate; the refined filtrate was concentrated under reduced pressure at 0.09 MPa and 60° C. to 1 / 3-1 / 4 of the primary filtrate, and then freeze-dried at -60° C. for 24 hours to obtain the banana fruit extract.

[0061] In order to prove the advantages of the technical solution provided by this application, the experimental data of the technical solution provided by this application are given below:

[0062] Experiment 1 Effect on collagen synthesis

[0063] In order to demonstrate the advantages of the technical solution provided by the present application, the test data of each composition provided in Examples 1 to 5, 6, 9, 12 and Comparative Examples 1 to 6 are given below.

[0064] Test method: The content of collagen type I, III and IV in fibroblasts was determined by enzyme-linked immunosorbent assay. After trypsin digestion, the fibroblasts were transferred to a 96-well culture plate, and the edge wells were filled with sterile phosphate buffer solution, and the plates were moved to an incubator for further culture. When the cells were completely attached to the wall and grew, 20 μL / well of the sample solution of the composition of Examples 1 to 5, 6, 9, and 12 Comparative Examples 1 to 6 were added in turn using a micropipette, and the same volume of 0.01 M PBS was added to the control group. After 48 hours of adding the sample, the content of type I, type III and type IV collagen was determined according to the instructions of the kit. There were 3 duplicate wells in each group, and ultrapure water was used as the control group, and its collagen synthesis was 100%. The test results are shown in Table 6.

[0065] Table 6 The amount of collagen synthesis of type I, type III and type IV

[0066]

[0067] Experiment 2 Skin elasticity test

[0068] Test samples: The experimental group is the composition of Examples 1 to 5, 6, 9, 12 and Comparative Examples 1 to 6 added to the same emulsion matrix, and the added amount is 5 wt %.

[0069] Testers: Healthy volunteers aged 30-40 were selected and randomly divided into groups of 5. The samples were applied twice a day, morning and evening. During the experiment, the subjects were not allowed to apply any other cosmetics on the experimental area.

[0070] Skin elasticity test: Use Cutometer MPA580, a skin elasticity meter, to test the skin condition in the first, second and fourth weeks respectively. The test site is the zygomatic area. During the measurement, the probe repeats the measurement 3 times in the same test area and takes the average value.

[0071] Skin elasticity growth rate W% = (NT-N0) / N0×100%

[0072] N0—Skin elasticity test value before application.

[0073] NT—Elasticity test value during the T period after application.

[0074] The test results are shown in Table 7:

[0075] Table 7 Skin elasticity growth rate

[0076]

[0077] Experiment 3: DPPH free radical scavenging test

[0078] Prepare a 0.2mmol / L DPPH solution with 95% ethanol and store it away from light. Prepare a 0.2mg / mL test solution with each composition provided in Examples 1 to 5, 6, 9, 12 and Comparative Examples 1 to 6, and immediately measure the absorbance (A) at a wavelength of 517nm with a 1cm cuvette, and record the absorbance as Ai. Then measure the absorbance after storing it away from light in a greenhouse for 30 minutes, and record it as Aj. The control test is an ethanol solution with only DPPH added, and its absorbance is recorded as A0. Calculate the free radical scavenging rate according to the following formula, and measure it three times in parallel to take the average value. The experimental results are shown in Table 8.

[0079]

[0080] Where: Ai is the absorbance of the reaction after adding the test substance;

[0081] Aj is the absorbance of the test solution itself;

[0082] A0 is the blank value without adding the test solution.

[0083] Table 8 DPPH free radical scavenging results

[0084] sample DPPH clearance rate (%) Blank matrix 9.17 Example 1 92.28 Example 2 77.38 Example 3 97.64 Example 4 90.58 Example 5 95.61 Example 6 96.50 Example 9 98.48 Example 12 99.79 Comparative Example 1 91.37 Comparative Example 2 90.02 Comparative Example 3 91.36 Comparative Example 4 87.70 Comparative Example 5 85.94 Comparative Example 6 98.31

[0085] Experiment 4: Moisturizing effect experiment

[0086] The specific test method is as follows:

[0087] Reference standard: Refer to QB / T 4256-2011 Guidelines for Evaluation of Moisturizing Efficacy of Cosmetics.

[0088] Test conditions: Capacitive skin moisture meter Corneometer CM825, test temperature 20-22°C, humidity 40-60%, test personnel are the same as in Experiment 2.

[0089] Test principle: The capacitance method is used to determine the moisture content of the stratum corneum of human skin. It is based on the significant difference in the dielectric constants of water and other substances. According to the different moisture content of the stratum corneum of the skin, the measured capacitance value of the skin is different, and its parameters can represent the skin moisture content (MMV).

[0090] Test samples: The experimental group is the composition provided in Examples 1 to 5, 6, 9, 12, and the composition of Comparative Examples 1-6 added to the same emulsion matrix, and the addition amount is 3wt%, respectively, to obtain test samples 1, 2, 3, 4, 5, 6, 9, 12, and controls 1-6.

[0091] Test method: Three fixed points are measured in each area in a certain order to obtain the average value. Then the sample to be tested and the control sample are applied to the test area (single application at a dosage of (2.0±0.1) mg / cm2). At 2h, 4h, 8h, and 24h, the probe is used to measure the skin moisture content of the test area, and the average value of each group at the corresponding time point is calculated. The calculation formula for the skin moisture content growth rate is:

[0092] Skin moisture content growth rate % = (MMVt-MMV0) / MMV0×100%

[0093] MMV0 - Skin MMV before application

[0094] MMVt——MMV of skin in the period t after application

[0095] The results are shown in Table 9.

[0096] Table 9 Growth rate of skin moisture content

[0097]

[0098]

[0099] According to the data in Tables 6-9, it can be found that the composition provided by the present invention can achieve a synergistic effect between the ingredients, can effectively and timely replenish the elastin required for the skin, thereby promoting the synthesis of collagen and restoring the elasticity of the skin. In addition, it also has the ability to scavenge free radicals to protect the skin from oxidative damage. By replenishing the necessary moisture for the skin, the composition can significantly increase the elasticity of the skin, making the skin more firm and elastic. At the same time, it can also effectively reduce wrinkles and fine lines, thereby achieving anti-aging and anti-wrinkle effects.

Claims

1. An anti-wrinkle and anti-aging composition containing a composite peptide, characterized in that: The invention mainly comprises a composite peptide, wherein the composite peptide comprises arginine / lysine polypeptide, acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, acetyl tetrapeptide-9 and acetyl tetrapeptide-11.

2. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 1, characterized in that: The composite peptide also includes a moisturizer and a solvent.

3. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 2, characterized in that: The moisturizing agent includes any one of sorbitol, polyethylene glycol, hexylene glycol, betaine, glycerin, propylene glycol, butylene glycol, methylpropanediol or pentylene glycol.

4. An anti-wrinkle and anti-aging composition containing a composite peptide according to any one of claims 1 to 4, characterized in that: The composite peptide is composed of the following components in percentage by mass: acetyl tetrapeptide-11 0.01% to 1%; acetyl tetrapeptide-9 0.01% to 1%; dipeptide diaminobutyryl benzylamide diacetate 0.01% to 1%; acetyl hexapeptide-8 0.01% to 1%; arginine / lysine polypeptide 0.001% to 1%; butanediol 20% to 50% and the balance water.

5. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 4, characterized in that: The composite peptide is composed of the following components in percentage by mass: acetyl tetrapeptide-110.03%; acetyl tetrapeptide-90.03%; dipeptide diaminobutyryl benzylamide diacetate 0.05%; acetyl hexapeptide-80.05%; arginine / lysine polypeptide 0.003%; butanediol 40% and the balance water.

6. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 1, characterized in that: The anti-wrinkle and anti-aging composition containing the complex peptide also includes 0.3N0.8% of banana fruit extract.

7. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 6, characterized in that: The anti-wrinkle and anti-aging composition containing the composite peptide also comprises 0.25-0.5% of Tremella fuciformis fruiting body extract and 0.3-0.8% of oregano flower oil.

8. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 7, characterized in that: The anti-wrinkle and anti-aging composition containing the composite peptide also includes 0.1-0.3% of Guangxi mandarin orange peel extract, 0.1-0.3% of dog rose fruit oil and 0.1-0.5% of black tea seed oil.

9. The anti-wrinkle and anti-aging composition containing a composite peptide according to claim 8, characterized in that: The plantain fruit extract is prepared by the following method: S1: peel fresh banana fruit and place it in an extraction container, then add deionized water in an amount of 1-2 times the weight of the banana fruit, and crush it to obtain the extract; S2: adding antioxidant to the extract, heating to 45-55°C, soaking for 6-8 hours to obtain a crude liquid; S3: Add 0.5-1.5wt% sodium chloride, 0.8-1.2wt% sodium dihydrogen phosphate, and 2-2.5wt% sucrose to the crude extract and mix well. 7 CFU / mL inoculation volume: 1×10 6 Inoculate Lactobacillus bulgaricus at an inoculum amount of 100 CFU / mL and Bacillus subtilis at an inoculum amount of 1×107 CFU / mL, ferment at 32-36° C. for 7-14 days, sterilize, and obtain a fermentation product; S4: adding chitosan to the fermented product and stirring for 1-3 hours, filtering through a 200-mesh sieve to obtain a primary filtrate, and then filtering through a 100-150 μm microporous filter membrane to obtain a refined filtrate; the refined filtrate is concentrated under reduced pressure to 1 / 3-1 / 4 of the primary filtrate, and freeze-dried to obtain the banana fruit extract.

10. Use of the anti-wrinkle and anti-aging composition containing the composite peptide according to claim 1 as a cosmetic additive.

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