Disposable ultraviolet flow cell and injection molding process of cell body thereof

Abstract

A light passing pipe is arranged in the vertical direction of a pool body, a liquid passing pipe is arranged in the horizontal direction of the pool body, the center part where the light passing pipe and the liquid passing pipe intersect vertically is a transmission space, two cavity windows which are symmetrical up and down are arranged in the space, and the contact face, making contact with liquid, of each cavity window is a cavity window face. The light path distance between the window surfaces of the two cavities is an actual light path; the upper end and the lower end of the light transmission pipe are connected with optical fiber connectors, collimating lenses are correspondingly arranged in the optical fiber connectors, and light beams flow through the light transmission pipe to form a light path. The invention also provides an injection molding process of the disposable ultraviolet flow cell, the cell body formed by injection molding of a polypropylene material can be used as a consumable in production, can be directly replaced without cleaning the interior of the cell body, and can realize one-time replacement of an ultraviolet detection pipeline when being matched with disposable chromatography, ultrafiltration and other equipment for use, so that the operation efficiency is effectively improved.

Description

Technical Field

[0001] The invention relates to a disposable ultraviolet flow cell and an injection molding process of a cell body thereof, which are used for measuring the change of light intensity passing through the ultraviolet flow cell to detect the change of component concentration in a sample, and belong to the technical field of biopharmaceuticals. Background Art

[0002] Ultraviolet absorption detector, also known as UV detector for short, is a detector designed based on the principle that solute molecules absorb ultraviolet light. Its working principle is Lambert-Beer law, that is, when a beam of monochromatic light passes through the flow cell, if the mobile phase does not absorb light, the absorbance A is proportional to the concentration C of the absorbing component and the optical path length L of the flow cell. Therefore, ultraviolet detectors are widely used in the fields of biopharmaceuticals, chemical processes and environmental protection. Its working principle is: ultraviolet light emitted from a specific light source enters the flow cell in the form of a parallel beam of a single wavelength after passing through a condenser and a cylindrical lens. After passing through the detection area, it is irradiated on the photosensitive sensor after passing through another cylindrical lens and a condenser. The different sample concentrations in the mobile phase cause the change in light intensity to be converted into a change in photocurrent. After amplification, it is input into a logarithmic converter or recorder to obtain a spectrum of sample concentration or optical density changes.

[0003] However, the existing UV flow cell has the following disadvantages: 1) The production efficiency of the traditional UV flow cell is low and the cost is high, and it cannot be quickly replaced during the detection process; 2) After completing each process, the UV flow cell needs to be cleaned each time to prevent liquid residue, which makes the operation complicated; 3) When the UV flow cell is matched with disposable chromatography, ultrafiltration and other equipment, since the UV flow cell needs to be retained, the complete pipeline cannot be replaced at one time, and the disassembly and replacement process is cumbersome.

[0004] Therefore, it is necessary to propose an injection molding process for a disposable UV flow cell and a cell body thereof to improve the applicability performance of the UV flow cell. Summary of the invention

[0005] The purpose of the invention of this application is to solve the above-mentioned problems and to provide a disposable UV flow cell and an injection molding process for its cell body, so as to solve the problems of high production cost and non-one-time replacement of the UV flow cell and to improve the comprehensive performance.

[0006] The technical solution of the present application is: a disposable ultraviolet flow cell, including a cell body in a cross structure, characterized in that: a light-passing tube is provided in the vertical direction of the cell body, and a liquid-passing tube is provided in the horizontal direction, and a liquid flow path is formed in the liquid-passing tube along its length direction. The central part of the light-passing tube and the liquid-passing tube which are perpendicular to each other is a transmission space, and two cavity windows which are symmetrical up and down are provided in the transmission space, and the contact surface of each cavity window in contact with the liquid is the cavity window surface, and the optical path distance between the two cavity window surfaces is the actual optical path. Optical fiber sockets are provided at the upper and lower ends of the light-passing tube, and each of the optical fiber sockets is connected to an optical fiber connector. Each of the optical fiber connectors is provided with a collimating lens for focusing and collimation, and the collimating lens is positioned and fixed on the corresponding light-passing tube, and the light beam flows through the light-passing tube to form an optical path.

[0007] Furthermore, in the above-mentioned disposable UV flow cell, the bottom of the optical fiber connector is provided with an optical fiber external thread structure, and the optical fiber external thread structure matches the optical fiber internal thread of the optical fiber socket.

[0008] Furthermore, in the above-mentioned disposable UV flow cell, a crimping ring is further provided between the optical fiber connector and the optical fiber socket.

[0009] Furthermore, in the above-mentioned disposable UV flow cell, a glue-coated ring groove structure is provided at the bottom of the optical fiber connector, and the glue-coated ring groove structure is assembled with the crimping ring to ensure a tight connection between the optical fiber connector and the crimping ring.

[0010] Furthermore, in the above-mentioned disposable UV flow cell, the collimating lens and the cell body are sealed and connected via a sealing ring.

[0011] The present application also provides a disposable UV flow cell body injection molding process, which is characterized by comprising the following steps:

[0012] Step (a): customizing the injection mold of the cell body, the mold is closed under the drive of the injection molding machine, and the mold is closed to prepare for injection molding;

[0013] Step (ii): injecting molten polypropylene material into the cavity of the injection mold at a pressure of 90 to 140 MPa, with a filling speed of 200 to 800 mm / s, filling the cavity to 93% to 96%. The shorter the filling time, the higher the molding efficiency.

[0014] Step (iii): continuously applying a pressure of 45 to 70 MPa to the injection mold for a holding time of 5 to 10 seconds to compact the melt to increase the density of the polypropylene material and compensate for the shrinkage behavior of the polypropylene material;

[0015] Step (iv): monitor the environmental changes of the injection mold, set up a monitoring network consisting of multiple temperature monitoring points and humidity monitoring points around it, the monitoring network adopts a multi-point distribution of temperature and humidity, and obtains the temperature and humidity values ​​and the temperature and humidity change data of each monitoring point in real time, so as to control the cooling system to cool at a fixed time, and then cool and solidify the polypropylene material in the injection mold;

[0016] Step (V): Open the injection mold after cooling, separate the molded cell body product from the injection mold, take the cell body product out of the injection mold, and perform a quality inspection on the cell body product to ensure that the cell body product meets the use requirements;

[0017] Step (six): The injection mold is closed again under the drive of the injection molding machine to prepare for the next injection molding operation of the polypropylene material.

[0018] Furthermore, in the injection molding process of the disposable UV flow cell, the temperature monitoring point is provided with a temperature sensor, which is one or more of a thermocouple sensor, a thermistor sensor, a resistance temperature detector (RTD) or an IC temperature sensor, and the temperature sensor includes a temperature acquisition module for obtaining a current temperature value in real time; the humidity monitoring point is provided with a humidity sensor, which is one or more of DHT11, DHT22, SHT11, HDC1080, SHT31, HS1101 or HYT-271, and the humidity sensor includes a humidity acquisition module for obtaining a current humidity value in real time.

[0019] Furthermore, in the above-mentioned injection molding process of the disposable UV flow cell, the cooling system is used to control the temperature of the injection mold to ensure that the plastic is properly molded in the mold, including a cooling water channel, a coolant circulation system and a temperature control device. The cooling pipeline is used to transport the coolant, the cooling element is responsible for distributing the coolant to various parts of the mold, and the temperature control system is used to monitor and control the temperature of the coolant to ensure the cooling effect of the mold, and the cooling system is one of linear cooling, spiral cooling, curved cooling or hot runner cooling.

[0020] The above-mentioned temperature and humidity change data of the present application is realized through the following technical solutions: a computer device, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor realizes analysis and processing of the values ​​of each temperature and humidity monitoring point when executing the computer program, so as to eliminate the interference of the surrounding environment and obtain accurate data; a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, wherein the computer program realizes the storage and retrieval of the analysis and processing values ​​of each temperature and humidity monitoring point when executed by the processor.

[0021] Compared with the prior art, after adopting the technical solution of the present invention, the cell body injection-molded by polypropylene material can be used as a consumable in production. The UV flow cell can be directly replaced without cleaning. When used in conjunction with disposable chromatography, ultrafiltration and other equipment, a one-time replacement of the UV detection pipeline can also be achieved, which not only saves production costs, but also greatly simplifies the operating steps.

[0022] Compared with the traditional injection molding process, the injection molding process adopted in the present invention adds a monitoring network with a multi-point distribution of temperature and humidity. The cooling and solidification of the pool model can be monitored in real time through each temperature and humidity monitoring point. The numerical values ​​of each temperature and humidity monitoring point can be analyzed and processed through computer equipment, and the cooling temperature and time can be adjusted according to the data, thereby improving the cooling efficiency and quality, thereby improving the injection molding rate of the pool body and saving material costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic structural diagram of a disposable ultraviolet flow cell of the present invention;

[0024] Figure 2 It is a schematic diagram of the decomposition of the disposable UV flow cell of the present invention;

[0025] Figure 3 It is a side view of the disposable UV flow cell of the present invention;

[0026] Figure 4 It is a schematic diagram of the enlarged structure of A of the present invention;

[0027] Figure 5 It is a cross-sectional schematic diagram of the present invention along the BB direction;

[0028] Figure 6 It is a schematic structural diagram of the cell body (without the optical fiber connector installed) of the present invention;

[0029] Figure 7 for Figure 6 Schematic cross-section of .

[0030] The meanings of the reference numerals in the figure are: 1-cell body, 2-liquid passage tube, 3-light passage tube, 31-optical fiber jack, 4-optical fiber connector, 5-crimping ring, 6-collimating lens, 7-cavity window, 8-cavity window surface, 9-light path, 10-actual light path, 11-liquid flow path. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further described below with reference to the accompanying drawings to make it easier to understand and grasp. The optical fiber connector 4, crimping ring 5 and collimating lens 6 involved therein are commonly used by ordinary technicians in the field, and there is no special requirement for them in this case.

[0032] like Figure 1 to Figure 7As shown, the present invention provides a disposable UV flow cell, comprising a cell body 1 in a cross structure, wherein the cell body 1 is provided with a light pipe 3 in the vertical direction and a liquid pipe 2 in the horizontal direction, wherein a liquid flow path 11 is formed in the liquid pipe 2 along its length direction.

[0033] According to the technical solution of the present invention, the central part of the light pipe 3 and the liquid pipe 2 which are perpendicular to each other is a transmission space, and two cavity windows 7 which are symmetrical up and down are arranged in the transmission space, and the contact surface of each cavity window 7 (that is, the cavity window 7 is a cylindrical convex shape) and the liquid is a cavity window surface 8, and the distance between the two cavity window surfaces 8 is the thickness of the liquid layer to be inspected, that is, the optical path distance of the two cavity window surfaces 8 is the actual optical path 10 of the disposable UV flow cell, and the actual optical path 10 is relatively long, which can improve the sensitivity of the UV detector. and resolution, the wider the sample concentration range that the UV detector can detect, the further improved performance indicators such as the linear range and response time of the UV detector, and the improved overall UV detection accuracy and efficiency; both upper and lower ends of the light tube 3 are provided with optical fiber sockets 31, each of the optical fiber sockets 31 is connected to an optical fiber connector 4, each of the optical fiber connectors is provided with a collimating lens 6 for focusing and collimation, and each collimating lens 6 is positioned and fixed on the corresponding light tube 3, and the light beam flows through the light tube 3 to form an optical path 9.

[0034] Preferably, in the above structure: the collimating lens 6 and the cell body 1 are sealed and connected via a sealing ring, thereby improving the tightness between the collimating lens 6 and the cell body 1 and being able to maintain a stable sealing effect under different working pressures and temperatures.

[0035] Preferably, in the above structure: the bottom of the optical fiber connector 4 is provided with an optical fiber external thread structure, which matches the optical fiber internal thread of the optical fiber socket 31 to facilitate disassembly and connection.

[0036] Specifically, a crimping ring 5 is provided between the optical fiber connector 4 and the optical fiber socket 31 to further improve the installation tightness of the optical fiber connector 4 and the optical fiber socket 31 .

[0037] More specifically, a glue-coated annular groove structure is provided at the bottom of the optical fiber connector 4 , and the glue-coated annular groove structure is adapted and assembled with the crimping ring 5 to ensure a tight connection between the optical fiber connector 4 and the crimping ring 5 .

[0038] In this way, the technical solution of the present invention is adopted. When in use, under normal working conditions, after the solution forms a liquid flow path and fills up the liquid passing tube 2, after the optical fiber is connected to the optical fiber connector 4 on the cell body 1, the incident optical fiber formed enters the cell body 1 through the upper light passing tube 3, and the light beam of the incident optical fiber is collimated and focused by a collimating lens 6 arranged on the upper light passing tube 3, so that the light beam passing through the transmission space is parallel light, and the parallel light passes through the window to transmit the solution, and the intensity of the light beam is attenuated, thereby measuring the absorbance; finally, the parallel light passes through the lower light passing tube 3, and another collimating lens 6 inside the lower light passing tube 3 converges the light beam, and the output optical fiber formed outputs a light signal to the ultraviolet detector, thereby detecting the measured substance and processing and analyzing the data of the corresponding detection results.

[0039] In addition, the present invention also provides a cell body injection molding process for an ultraviolet flow cell, and various embodiments are as follows.

[0040] [Example 1]

[0041] First, the injection mold of the pool body 1 needs to be customized. The mold is closed under the drive of the injection molding machine and is prepared for injection molding by mold clamping. At a pressure of 140MPa, the molten polypropylene material is injected into the cavity of the injection mold. The filling speed is 800mm / s, and the cavity is filled to 96%. The shorter the filling time, the higher the molding efficiency. The pressure of 70MPa is continuously applied to the injection mold, and the holding time is 10s. The melt is compacted to increase the density of the polypropylene material and compensate for the shrinkage behavior of the polypropylene material. The environmental changes of the injection mold are monitored, and a monitoring network consisting of multiple temperature monitoring points (i.e., the temperature monitoring points are provided with IC temperature sensors to obtain temperature values) and humidity monitoring points (i.e., the humidity monitoring points are provided with HYT-271 to obtain humidity values) is set around it. The monitoring network adopts a multi-point distribution of temperature and humidity, and obtains the temperature and humidity values ​​and the temperature and humidity change data of each monitoring point in real time. The value of each temperature and humidity monitoring point is analyzed and processed by computer equipment. It includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the computer program is executed by the processor, the storage and retrieval of the analysis and processing values ​​of each temperature and humidity monitoring point are realized through a computer-readable storage medium, so as to capture the temperature and humidity in all directions to determine the cooling parameters, determine whether the temperature change achieves the cooling effect of the polypropylene material, and eliminate humidity interference, adjust the cooling temperature, and maintain constant temperature cooling, thereby controlling the curved cooling and timing cooling in the cooling system, and then cooling and solidifying the polypropylene material in the injection mold; opening the injection mold after cooling, separating the molded pool body product from the injection mold, taking the pool body product out of the injection mold, and performing a quality inspection on the pool body product to ensure that the pool body product meets the use requirements (after the injection molding process is completed, the injection mold is closed again under the drive of the injection molding machine to prepare for the next injection molding operation of the polypropylene material), and the pool body 1 can be used in conjunction with disposable chromatography, ultrafiltration and other equipment.

[0042] [Example 2]

[0043] First, the injection mold of the pool body 1 is customized. The mold is closed under the drive of the injection molding machine and prepared for injection molding by mold closing: under a pressure of 110MPa, the molten polypropylene material is injected into the cavity of the injection mold with a filling speed of 600mm / s, and the cavity is filled to 95%; a pressure of 50MPa is continuously applied to the injection mold, and the holding time is 8s to compact the melt to increase the density of the polypropylene material: a monitoring network consisting of multiple temperature monitoring points (i.e., the temperature monitoring points are provided with thermocouple sensors to obtain temperature values) and humidity monitoring points (i.e., the humidity monitoring points are provided with DHT22 to obtain humidity values) is set around it to monitor the environmental changes of the injection mold. The monitoring network adopts a multi-point distribution of temperature and humidity to obtain the temperature and humidity values ​​and the temperature and humidity change data of each monitoring point in real time, and the analysis of the values ​​of each temperature and humidity monitoring point is realized through computer equipment. and processing, which includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the computer program is executed by the processor, the storage and retrieval of the analysis and processing values ​​of each temperature and humidity monitoring point are realized through a computer-readable storage medium, so as to capture the temperature and humidity in all directions to determine the cooling parameters, determine whether the temperature change achieves the cooling effect of the polypropylene material, and eliminate humidity interference, adjust the cooling temperature, and maintain constant temperature cooling, thereby controlling the linear cooling in the cooling system to perform timed cooling of the model, and then completely cool and solidify the polypropylene material in the injection mold; open the injection mold after cooling, separate the formed cell body 1 product from the injection mold, take the cell body 1 product out of the injection mold, and perform a quality inspection on the cell body 1 product to ensure that the cell body product meets the use requirements, and use the cell body 1 in conjunction with disposable chromatography, ultrafiltration and other equipment.

[0044] [Example 3]

[0045] First, the injection mold of the pool body 1 is customized. The mold is closed under the drive of the injection molding machine and prepared for injection molding by mold closing: under a pressure of 90MPa, the molten polypropylene material is injected into the cavity of the injection mold with a filling speed of 200mm / s, and the cavity is filled to 93%; a pressure of 45MPa is continuously applied to the injection mold with a holding time of 5s to compact the melt to increase the density of the polypropylene material: a monitoring network consisting of multiple temperature monitoring points (i.e., the temperature monitoring points are provided with thermocouple sensors to obtain temperature values) and humidity monitoring points (i.e., the humidity monitoring points are provided with HDC1080 to obtain humidity values) is set around it to monitor the environmental changes of the injection mold. The monitoring network adopts a multi-point distribution of temperature and humidity to obtain the temperature and humidity values ​​and the temperature and humidity change data of each monitoring point in real time, and the analysis and processing of the values ​​of each temperature and humidity monitoring point are realized through computer equipment, which includes The invention comprises a memory, a processor and a computer program stored in the memory and executable on the processor. When the computer program is executed by the processor, the storage and retrieval of the analysis and processing values ​​of each temperature and humidity monitoring point are realized through a computer-readable storage medium, so as to capture the temperature and humidity in an all-round way to determine the cooling parameters, determine whether the temperature change achieves the cooling effect of the polypropylene material, and eliminate humidity interference, adjust the cooling temperature and maintain constant temperature cooling, thereby controlling the linear cooling in the cooling system to perform timed cooling of the model, and then completely cool and solidify the polypropylene material in the injection mold; open the injection mold after cooling, separate the formed cell body 1 product from the injection mold, take the cell body 1 product out of the injection mold, and perform a quality inspection on the cell body 1 product to ensure that the cell body product meets the use requirements and can be used as a consumable with low cost. The cell body 1 can be used in conjunction with disposable chromatography, ultrafiltration and other equipment.

[0046] Among the technical solutions of the present invention, the disposable polypropylene injection-molded pool body and the symmetrical light-transmitting structure arranged inside it are the technical key of this case. Figure 5 The focus is on the relevant components involved in the formation of the optical path 9. Since the cell body 1 is a symmetrical structure, there is no need to consider the direction of the optical fiber connector 4 during installation and use, which is convenient for the staff to adapt and install. For components such as the collimating lens 6, ordinary technicians in this field can perform conventional settings based on existing technologies. This case has no special requirements for its model selection and combined use.

[0047] From the above description, it can be found that compared with the prior art, after adopting the technical solution of the present invention, the circulation pool body is processed and formed by injection molding, which can save costs and be used as consumables. The circulation pool has a high degree of hygiene and reduces the cleaning process in the actual process. When encountering residual impurities and difficult cleaning, the pool body can be directly replaced. The operation is simple and convenient. When used in conjunction with disposable chromatography, ultrafiltration and other equipment, a one-time replacement of the ultraviolet detection pipeline can also be achieved.

[0048] The technical scheme, working process and implementation effect of the present invention are described in detail above. It should be noted that what is described is only a typical example of the present invention. In addition, the present invention may also have many other specific implementation methods. Any technical scheme formed by equivalent replacement or equivalent transformation falls within the scope of protection required by the present invention.

Claims

1. A disposable ultraviolet flow cell, comprising a cell body (1) in a cross structure, characterized in that: The pool body (1) is provided with a light pipe (3) in the vertical direction and a liquid pipe (2) in the horizontal direction; the central part of the light pipe (3) and the liquid pipe (2) which are perpendicular to each other is a transmission space, and two cavity windows (7) which are symmetrical in upper and lower directions are provided in the transmission space; the light pipe (3) is provided with optical fiber sockets (31) at both upper and lower ends, each optical fiber socket (31) is connected to an optical fiber connector (4), and each optical fiber connector (4) is provided with a collimating lens (6) for focusing and collimating light, and each collimating lens (6) is positioned and fixed on the corresponding light pipe (3).

2. The disposable UV flow cell according to claim 1, characterized in that: The bottom of the optical fiber connector (4) is provided with an optical fiber external thread structure, and the optical fiber external thread structure matches the optical fiber internal thread of the optical fiber socket (31).

3. The disposable UV flow cell according to claim 2, characterized in that: A crimping ring (5) is also provided between the optical fiber connector (4) and the optical fiber socket (31).

4. The disposable UV flow cell according to claim 3, characterized in that: The bottom of the optical fiber connector (4) is provided with a glue-coated ring groove structure, and the glue-coated ring groove structure is adapted to be assembled with the crimping ring (5).

5. The disposable UV flow cell according to claim 1, characterized in that: The collimating lens (6) and the cell body (1) are sealed and connected via a sealing ring.

6. The cell body injection molding process of the disposable UV flow cell according to any one of claims 1 to 5, characterized in that: The following steps are involved: Step S1: customizing an injection mold for the cell body (1), the mold is closed under the drive of an injection molding machine, and is prepared for injection molding by mold closing; Step S2: injecting molten polypropylene material into the cavity of the injection mold at a pressure of 90 to 140 MPa, with a filling speed of 200 to 800 mm / s, until the cavity is filled to 93% to 96%; Step S3: continuously applying a pressure of 45 to 70 MPa to the injection mold for a holding time of 5 to 10 seconds to compact the melt to increase the density of the polypropylene material and compensate for the shrinkage behavior of the polypropylene material; Step S4: monitor the environmental changes of the injection mold, and set a monitoring network consisting of multiple temperature monitoring points and humidity monitoring points around it. The monitoring network adopts a multi-point distribution structure of temperature and humidity crossover to obtain the temperature and humidity values ​​and the temperature and humidity change data of each monitoring point in real time, so as to control the cooling system to cool at a fixed time, and then cool and solidify the polypropylene material in the injection mold; Step S5: opening the injection mold after cooling, separating the molded cell body (1) product from the injection mold, taking the cell body (1) product out of the injection mold, and performing a quality inspection on the cell body (1) product to ensure that the cell body (1) product meets the use standards; Step S6: The injection mold is closed again under the drive of the injection molding machine to prepare for the next injection molding operation of the polypropylene material.

7. The cell body injection molding process of the disposable UV flow cell according to claim 6, characterized in that: In step S4, the temperature monitoring point is provided with a temperature sensor, and the temperature sensor includes a temperature acquisition module for acquiring the current temperature value in real time; the humidity monitoring point is provided with a humidity sensor, and the humidity sensor includes a humidity acquisition module for acquiring the current humidity value in real time.

8. The cell body injection molding process of the disposable UV flow cell according to claim 6, characterized in that: In step S4, the cooling system is one of linear cooling, spiral cooling, curved cooling or hot runner cooling.

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