A kind of biological organic fertilizer and its production method and application

Abstract

The present invention provides a kind of biological organic fertilizer and its production method and application, belongs to the field of biological organic fertilizer technology, and the production method of the biological organic fertilizer comprises the following steps: S1, by weight, 0.8-1 parts of seaweed crushed material, 7.5-8 parts of straw and 1-1.3 parts of human and animal feces are mixed to obtain a basic material; S2, a composite bacterial agent is added to the basic material obtained by S1, the mass of the composite bacterial agent is 0.2-0.3% of the mass of the basic material, mixed, uniformly ventilated, composted for 25-28d, and a biological organic fertilizer is obtained; the composite bacterial agent includes Bacillus licheniformis, nitrogen-fixing bacteria and micro-rhizomucor. The present invention ensures that the fertilizer release rate of the obtained fertilizer is fast and the action time is long, and the growth needs of short-growth cycle vegetables and long-growth cycle vegetables can be better taken into account.

Description

Technical Field

[0001] The invention belongs to the technical field of bio-organic fertilizers, and in particular relates to a bio-organic fertilizer and a production method and application thereof. Background Art

[0002] Bio-organic fertilizer is a kind of fertilizer that combines the effects of microbial fertilizer and organic fertilizer, and is composed of specific functional microorganisms and harmless, composted and decomposed organic materials. The raw materials of existing bio-organic fertilizers are extensive, including agricultural waste (such as straw, soybean meal, etc.), livestock and poultry manure (such as chicken manure, cattle and sheep manure, etc.), industrial waste (such as wine lees, vinegar lees, etc.) and domestic garbage, etc.; the main production processes are flat windrow composting process and trough aerobic fermentation process; it can provide comprehensive nutrients, including N, P, K and other macroelements and a variety of trace elements. Long-term use of bio-organic fertilizer can significantly increase the content of soil organic matter, enhance the soil's fertilizer and water retention capacity, and improve soil structure. The application of bio-organic fertilizer can also significantly increase crop yield and quality. The beneficial microorganisms in bio-organic fertilizer can also improve the root microbial community of crops and improve the plant's resistance to diseases and insects.

[0003] The release speed and duration of the fertilizer effect of existing bio-organic fertilizers vary depending on the product type and ingredients. For vegetables with a short growth cycle (such as leafy vegetables) or fast-growing crops, you can choose bio-organic fertilizers with a faster fertilizer release rate. This type of fertilizer usually contains a higher proportion of soluble organic matter and microbial agents, which can quickly provide the nutrients needed by crops and promote their rapid growth. For crops with a longer growth cycle (such as fruit trees, melons, etc.), you can choose bio-organic fertilizers that have a relatively slow fertilizer release rate but a longer duration. Crops with different growth cycles need to choose different bio-organic fertilizers, which cannot be well balanced. Summary of the invention

[0004] In order to solve the problems existing in the background technology, the present invention provides a biological organic fertilizer and its production method and application, which ensures that the fertilizer effect of the fertilizer is released quickly and for a long time, and can well take into account the growth needs of vegetables with different growth cycles (short growth cycle and long growth cycle).

[0005] In order to achieve the above object, the present invention provides a method for producing a bio-organic fertilizer, comprising the following steps:

[0006] S1. Mix 0.8-1 parts of seaweed crushed material, 7.5-8 parts of straw and 1-1.3 parts of human and animal feces by weight to obtain a basic material;

[0007] S2. Add a composite bacterial agent to the basic material obtained in S1, wherein the mass of the composite bacterial agent is 0.2-0.3% of the mass of the basic material, mix well, ventilate at a constant speed, and compost for 25-28 days to obtain a biological organic fertilizer;

[0008] The composite bacterial agent comprises Bacillus licheniformis, nitrogen-fixing bacteria and Rhizomucor microplus.

[0009] Further, in S1, 0.9 parts of seaweed crushed material, 7.8 parts of straw and 1.1 parts of human and animal feces are mixed uniformly by weight to obtain a basic material.

[0010] Furthermore, the production method of the seaweed crushed material is as follows:

[0011] S11, drying the seaweed to a water content of 55-60%, and then chopping the seaweed to obtain shredded material;

[0012] S12, adding sodium alginate to the crushed material obtained in S11, wherein the mass of sodium alginate is 7-9% of the mass of the crushed material, and mixing well to obtain seaweed crushed material.

[0013] Furthermore, in the composite bacterial agent, the content of live bacteria of Bacillus licheniformis is 8*10 6 -1*10 7 CFU / g, the live bacteria content of nitrogen-fixing bacteria is 5*10 6 -7*10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 1*10 6 -3*10 6 CFU / g.

[0014] Furthermore, in the composite bacterial agent, the content of live bacteria of Bacillus licheniformis is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 2.2*10 6 CFU / g.

[0015] Furthermore, in S2, the specific operation of uniform ventilation is: ventilation for 12-15 minutes every 1 hour, and the ventilation volume is 0.32-0.35L / (h·kg).

[0016] In a second aspect, the present invention provides a bio-organic fertilizer produced by the above-mentioned production method.

[0017] In a third aspect, the present invention provides an application of a bio-organic fertilizer, which is prepared by the above-mentioned production method, and is suitable for short-growth cycle vegetables and long-growth cycle vegetables; the short-growth cycle vegetables include water spinach, Chinese cabbage, lettuce, romaine lettuce, spinach, and rapeseed; the long-growth cycle vegetables include cabbage, radish, cucumber, and tomato.

[0018] This application has the following beneficial effects:

[0019] 1. In the preparation of the biological organic fertilizer of the present invention, seaweed crushed material and micro-rhizomucor are used together, and there is a synergistic effect between the two, which can synergistically improve the overall fertilization effect of the obtained fertilizer on the short growth cycle crop (water spinach), so that the fresh weight increase of the short growth cycle crop (water spinach) in each time period after planting is significantly increased, and finally the fresh weight of water spinach 30 days after planting is significantly increased; and can synergistically improve the overall fertilization effect of the obtained fertilizer on the long growth cycle crop (cabbage), so that the fresh weight increase of the long growth cycle crop (cabbage) in each time period after planting is significantly increased, and finally the fresh weight of the cabbage 50 days after planting is significantly increased.

[0020] 2. In the preparation of the seaweed crushed material of the present invention, the addition of raw seaweed can increase the carbon-nitrogen ratio (C / N) of the basic material, slow down the speed at which microorganisms decompose these substances, and thus delay the release of fertilizer effect; the addition of raw sodium alginate can increase the content of sodium alginate, slow down the diffusion rate of fertilizer effect components into the soil, and thus prolong the action time of the fertilizer. At the same time, the present invention adds microscopic Rhizomucor, which has a strong decomposition ability, can accelerate the decomposition and transformation of organic matter, and increase the release rate of fertilizer effect (also shorten the action time of the fertilizer). The two (seaweed crushed material and microscopic Rhizomucor) cooperate, complement each other, and work synergistically, so that the prepared fertilizer can well take into account the growth needs of vegetables with different growth cycles (short growth cycle and long growth cycle). BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 , a comparative trend chart of the test data of each stage of the increase in fresh weight of water spinach in the blank control group, the embodiment 1 group and the comparative examples 1 to 3 groups of the present invention;

[0022] Figure 2 , a comparative trend chart of the total amount of fresh weight increase test data of the blank control group, Example 1 to Example 5 groups and Comparative Example 1 to Comparative Example 3 groups of water spinach planted 30 days after planting in Test Example 1 of the present invention;

[0023] Figure 3 , a comparative trend chart of the test data of each stage of the increase in fresh weight of Chinese cabbage in the blank control group, the embodiment 1 group and the comparative examples 1 to 3 groups in the test example 2 of the present invention;

[0024] Figure 4 , a comparative trend chart of the total amount of fresh weight increase test data of the blank control group, Example 1 to Example 5 groups and Comparative Example 1 to Comparative Example 3 groups of cabbage 50 days after planting in Test Example 2 of the present invention. DETAILED DESCRIPTION

[0025] The present application is further described in detail below with reference to the embodiments.

[0026] Unless otherwise specified, the raw materials used in the examples and comparative examples of the present application are all commercially available.

[0027] Example 1: (1) Prepare seaweed fragments, the preparation method is as follows:

[0028] S11, drying the seaweed at 55° C. to a water content of 58%, and then chopping the seaweed to obtain chop.

[0029] S12, adding sodium alginate to the crushed material obtained in S11, wherein the mass of sodium alginate is 8% of the mass of the crushed material, and mixing well to obtain seaweed crushed material.

[0030] Among them, sodium alginate (99%) was purchased from Qingdao Huazhiyuan Food Additive Co., Ltd.

[0031] (2) A method for producing a bio-organic fertilizer, comprising the following steps:

[0032] S1. Mix 0.9 parts of seaweed crushed material, 7.8 parts of straw and 1.1 parts of human and animal feces by weight to obtain a basic material. The straw is specifically corn straw (crushed).

[0033] S2. Add a composite bacterial agent to the base material obtained in S1. The mass of the composite bacterial agent is 0.25% of the mass of the base material. The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 2.2*10 6 CFU / g. After mixing, uniform ventilation is performed for composting. The specific operation of uniform ventilation is: ventilation for 13 minutes every 1 hour, and the ventilation volume is 0.33L / (h·kg). After 26 days of composting, biological organic fertilizer is obtained.

[0034] Among them, Bacillus licheniformis (RN-86412), nitrogen-fixing bacteria (RN-62434) and Rhizomucor pusillus (RN-11030) were purchased from Noan Gene Technology (Wuhan) Co., Ltd.

[0035] Azotobacteria (RN-62434) are obligate aerobic bacteria that require oxygen to fix nitrogen, converting atmospheric nitrogen into ammonia nitrogen that can be absorbed and utilized by plants. Therefore, azotobacteria usually require an adequate supply of oxygen during the fermentation process.

[0036] Bacillus licheniformis (RN-86412) is a facultative anaerobe, which means it can grow in both aerobic and anaerobic conditions, but prefers aerobic environments. In aerobic conditions, it can quickly consume free oxygen in the ecological environment, produce biological oxygen deprivation, create a hypoxic environment, and promote the growth of anaerobic bacteria.

[0037] Rhizomucor microsporus (RN-11030) is a fungus that relies heavily on anaerobic conditions for growth and metabolism. Rhizomucor microsporus is able to grow and produce metabolites better in anoxic or hypoxic environment.

[0038] Uniform ventilation (i.e., continuous equal amount of oxygen supply at the same speed) is adopted. In the early and middle stages of composting, Bacillus licheniformis and nitrogen-fixing bacteria are in a rapid growth period, with high respiration intensity and large oxygen uptake rate. As the concentration of bacteria continues to rise, the oxygen consumption continues to increase, making the composting fermentation environment in a low oxygen state, which is conducive to the participation of Rhizomucor microscopic in fermentation. In the late stage of composting, after the bacterial growth enters the stable period, the bacterial respiration intensity begins to weaken and the oxygen uptake rate decreases, but the oxygen supply of the composting fermentation environment remains unchanged, so that the composting fermentation environment is in an oxygen-rich state, thereby weakening the fermentation progress of Rhizomucor microscopic. In other words, in the early and middle stages of fermentation, Bacillus licheniformis, nitrogen-fixing bacteria and Rhizomucor microscopic can all grow rapidly, while in the late stage of fermentation, Bacillus licheniformis and nitrogen-fixing bacteria grow normally, and Rhizomucor microscopic is curbed, so as to achieve the purpose of effectively controlling the live bacterial content of Rhizomucor microscopic at a relatively low level (lower than the live bacterial content of Bacillus licheniformis and the live bacterial content of nitrogen-fixing bacteria).

[0039] Example 2: The difference between this example and Example 1 is that: (1) seaweed crushed material is prepared, and the preparation method thereof is as follows:

[0040] S11, drying the seaweed at 55° C. until the water content is 55%, and then chopping the seaweed to obtain chop.

[0041] S12, adding sodium alginate to the crushed material obtained in S11, wherein the mass of sodium alginate is 7% of the mass of the crushed material, and mixing well to obtain seaweed crushed material.

[0042] Example 3: The difference between this example and Example 1 is that: (1) seaweed crushed material is prepared, and the preparation method thereof is as follows:

[0043] S11, drying the seaweed at 55° C. until the water content is 60%, and then chopping the seaweed to obtain chop.

[0044] S12, adding sodium alginate to the crushed material obtained in S11, wherein the mass of sodium alginate is 9% of the mass of the crushed material, and mixing well to obtain seaweed crushed material.

[0045] Embodiment 4: The difference between this embodiment and embodiment 1 is that: (2) A method for producing a biological organic fertilizer comprises the following steps:

[0046] S1. Mix 0.8 parts of seaweed crushed material, 7.5 parts of straw and 1 part of human and animal feces by weight to obtain a basic material. The straw is specifically corn straw (crushed).

[0047] S2. Add a composite bacterial agent to the base material obtained in S1. The mass of the composite bacterial agent is 0.2% of the mass of the base material. The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 2.2*10 6 CFU / g. After mixing, ventilate at a constant speed. The specific operation of uniform ventilation is: ventilate for 12 minutes every 1 hour, and the ventilation volume is 0.35L / (h·kg). Compost for 25 days to obtain biological organic fertilizer.

[0048] Embodiment 5: The difference between this embodiment and embodiment 1 is that: (2) a method for producing a biological organic fertilizer comprises the following steps:

[0049] S1. Mix 1 part of seaweed crushed material, 8 parts of straw and 1.3 parts of human and animal feces by weight to obtain a basic material. The straw is specifically corn straw (crushed).

[0050] S2. Add a composite bacterial agent to the base material obtained in S1. The mass of the composite bacterial agent is 0.3% of the mass of the base material. The live bacterial content of Bacillus licheniformis in the composite bacterial agent is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 2.2*10 6 CFU / g. After mixing, ventilate at a constant speed. The specific operation of uniform ventilation is: ventilate for 15 minutes every 1 hour, and the ventilation volume is 0.32L / (h·kg). Compost for 28 days to obtain biological organic fertilizer.

[0051] Comparative Example 1: The difference between this comparative example and Example 1 is that in the production of biological organic fertilizer, seaweed fragments are deleted and Rhizomucor microplus is deleted.

[0052] Specifically, a method for producing a bio-organic fertilizer comprises the following steps:

[0053] S1. Mix 7.8 parts of straw and 1.1 parts of human and animal feces by weight to obtain a basic material.

[0054] S2. Add a composite bacterial agent to the base material obtained in S1. The mass of the composite bacterial agent is 0.25% of the mass of the base material. The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6 CFU / g. After mixing, uniform ventilation is performed for composting. The specific operation of uniform ventilation is: ventilation for 13 minutes every 1 hour, and the ventilation volume is 0.33L / (h·kg). After 26 days of composting, biological organic fertilizer is obtained.

[0055] Comparative Example 2: The difference between this comparative example and Example 1 is that seaweed fragments are deleted in the production of biological organic fertilizer.

[0056] Specifically, a method for producing a bio-organic fertilizer comprises the following steps:

[0057] S1. Mix 7.8 parts of straw and 1.1 parts of human and animal feces by weight to obtain a basic material.

[0058] S2. Add a composite bacterial agent to the base material obtained in S1. The mass of the composite bacterial agent is 0.25% of the mass of the base material. The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 2.2*10 6 CFU / g. After mixing, uniform ventilation is performed for composting. The specific operation of uniform ventilation is: ventilation for 13 minutes every 1 hour, and the ventilation volume is 0.33L / (h·kg). After 26 days of composting, biological organic fertilizer is obtained.

[0059] Comparative Example 3: The difference between this comparative example and Example 1 is that Rhizomucor microplus is deleted in the production of biological organic fertilizer.

[0060] Specifically, a method for producing a bio-organic fertilizer comprises the following steps:

[0061] S1. Mix 0.9 parts of seaweed crushed material, 7.8 parts of straw and 1.1 parts of human and animal feces by weight to obtain a basic material.

[0062] S2. Add a composite bacterial agent to the base material obtained in S1. The mass of the composite bacterial agent is 0.25% of the mass of the base material. The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8.7*10 6 CFU / g, the content of live nitrogen-fixing bacteria is 5.9*10 6CFU / g. After mixing, uniform ventilation is performed for composting. The specific operation of uniform ventilation is: ventilation for 13 minutes every 1 hour, and the ventilation volume is 0.33L / (h·kg). After 26 days of composting, biological organic fertilizer is obtained.

[0063] Test example 1: Test project: Growth test of water spinach, a vegetable with a short growth cycle (fresh weight).

[0064] Test subjects: Fertilizers were prepared according to Examples 1 to 5 and Comparative Examples 1 to 3.

[0065] Test method and content: 9 groups were set up, including 1 blank control group, 5 embodiment groups and 3 comparative example groups.

[0066] Each group included three pots, each pot was filled with 3 kg of soil, and each pot was planted with a 5-day-old water spinach seedling. The fresh weight was tested on the day of planting and on the 10th, 20th and 30th days after planting, and the fresh weight increase of the water spinach in the first 10 days, the second 10 days and the third 10 days after planting was calculated and recorded. Before planting, the soil of each pot in each group was evenly mixed with the corresponding fertilizer (except for the blank control group), and the amount of fertilizer added was 0.9% of the soil. Each time the fresh weight was measured, a pot in the corresponding group was selected and weighed directly with an analytical balance.

[0067] Test results: See Table 1.

[0068] Table 1. Test data of test example 1

[0069]

[0070] Result analysis: Analyze the blank control, Example 1-Example 5 and combine the data in Table 1 and Figure 1-Figure 2 It can be seen that after applying the fertilizer prepared by the present invention for 30 days, the weight of water spinach reaches more than 70.1g, which is significantly greater than 49.2g without fertilizer (blank control). Moreover, compared with no fertilizer (white control), the application of the fertilizer prepared by the present invention (Example 1-Example 5) can significantly increase the increase in fresh weight of water spinach in three time periods after planting (the first 10 days, the second 10 days and the third 10 days). It shows that the fertilizer prepared by the present invention can well meet the growth requirements of water spinach at various growth stages of short growth cycle vegetables, thereby significantly increasing the fresh weight of water spinach.

[0071] Analyze the blank control, Example 1 and Comparative Examples 1-3 and combine the data in Table 1 and Figure 1-Figure 2By comparing Comparative Example 1 and Comparative Example 2, it can be seen that compared with Comparative Example 1, Rhizomucor microphylla is added to the composite bacterial agent used for composting in Comparative Example 2, resulting in a significant increase in the fresh weight increase of water spinach within the first 10 days after planting, but a continuous decrease in the fresh weight increase within the second 10 days and the third 10 days; ultimately resulting in the total fresh weight increase of water spinach in Comparative Example 2 after 30 days of planting being less than that in Comparative Example 1. This shows that the addition of Rhizomucor microphylla can improve the initial effect of the fertilizer, but will also cause a decrease in the effect of the fertilizer in the middle and late stages of application, ultimately resulting in a decrease in the overall effect of the fertilizer.

[0072] This is because the addition of Rhizomucor alone can increase the release rate of the fertilizer effect of the prepared fertilizer, causing the fertilizer effect to be released too quickly in the early stages of crop growth, resulting in a waste of fertilizer effect in the early stages of growth, and insufficient supply capacity of fertilizer effect in the middle and late stages of crop growth.

[0073] By comparing Comparative Example 1 and Comparative Example 3, it can be seen that compared with Comparative Example 1, the self-made seaweed crushed material of the present invention is added to the base material of Comparative Example 3, resulting in a significant decrease in the fresh weight increase of water spinach in the first 10 days and the second 10 days after planting, and an increase in the fresh weight increase in the third 10 days; ultimately resulting in the total fresh weight increase of water spinach in Comparative Example 3 after 30 days of planting being less than that in Comparative Example 1. This indicates that when the prepared fertilizer is applied to crops with a short growth cycle (30 days), the addition of seaweed crushed material can reduce the effect of the prepared fertilizer in the early and middle stages of application, and at the same time improve the effect of the fertilizer in the later stages of application, ultimately resulting in a decrease in the overall effect of the fertilizer.

[0074] This is because the addition of seaweed fragments alone will, on the one hand, increase the carbon-nitrogen ratio (C / N) of the base material, causing the rate at which microorganisms decompose substances during the composting process to slow down, affecting the degree of decomposition, and affecting the release rate of the fertilizer effect during subsequent application and use, resulting in the inability to fully meet the needs of the early and middle stages of crop growth; on the other hand, the high content of sodium alginate in the seaweed fragments will also slow down the diffusion rate of fertilizer ingredients into the soil, resulting in poor diffusion effect (uniformity and sufficiency) of the fertilizer effect in the early and middle stages of crop growth.

[0075] Combined with Example 1 for comparison, it can be seen that compared with Comparative Example 1, the homemade seaweed crushed materials of the present invention are added to the basic materials of Example 1, and Rhizomucor microphylla is added to the composite bacterial agent used for composting. As a result, the fresh weight increase of water spinach in the first 10 days, the second 10 days and the third 10 days after planting is increased; finally, the total amount of fresh weight increase of water spinach in Example 1 after 30 days of planting is also significantly greater than that of Comparative Example 1. It shows that in the preparation of the fertilizer of the present invention, seaweed crushed materials and Rhizomucor microphylla are used together, and there is a synergistic effect between the two, which can synergistically improve the overall effect of the obtained fertilizer on short growth cycle crops (water spinach).

[0076] Test Example 2: Test item: Growth test of cabbage, a vegetable with a long growth cycle (fresh weight).

[0077] Test subjects: Fertilizers were prepared according to Examples 1 to 5 and Comparative Examples 1 to 3.

[0078] Test method and content: 9 groups were set up, including 1 blank control group, 5 embodiment groups and 3 comparative example groups.

[0079] Each group included five pots, each pot was filled with 6 kg of soil, and each pot was planted with a 6-day-old cabbage seedling. The fresh weight was tested on the day of planting and on the 10th, 20th, 30th, 40th and 50th days after planting. The fresh weight increase of the cabbage in the first 10 days, the second 10 days, the third 10 days, the fourth 10 days and the fifth 10 days after planting was calculated and recorded. Before planting, the soil of each pot in each group was evenly mixed with the corresponding fertilizer (except for the blank control group), and the amount of fertilizer added was 1.3% of the soil. Each time the fresh weight was measured, a pot in the corresponding group was selected and weighed directly with an analytical balance.

[0080] Test results: See Table 2.

[0081] Table 2. Test data of Experimental Example 2

[0082]

[0083] Result analysis: Analyze the blank control, Example 1-Example 5 and combine the data in Table 1 and Figure 3-Figure 4 It can be seen that after 50 days of planting with the fertilizer prepared by the present invention, the weight of the cabbage reached more than 458.4g, which is significantly greater than 366.9g without fertilizer (blank control). In addition, compared with no fertilizer (blank control), the application of the fertilizer prepared by the present invention (Example 1-Example 5) can significantly increase the increase in fresh weight of cabbage in five time periods after planting (the first 10 days, the second 10 days, the third 10 days, the fourth 10 days and the fifth 10 days). It shows that the fertilizer prepared by the present invention can well meet the growth requirements of cabbage at various growth stages of long growth cycle vegetables, thereby significantly increasing the fresh weight of cabbage.

[0084] Analyze the blank control, Example 1 and Comparative Examples 1-3 and combine the data in Table 1 and Figure 3-Figure 4By comparing Comparative Example 1 and Comparative Example 2, it can be seen that compared with Comparative Example 1, Rhizomucor microphylla is added to the composite bacterial agent used in the composting of Comparative Example 2, resulting in a significant increase in the fresh weight increase of the cabbage in the first 10 days and the second 10 days after planting, while the fresh weight increase in the third 10 days, the fourth 10 days and the fifth 10 days continuously decreases; ultimately resulting in the total fresh weight increase of the cabbage in Comparative Example 2 after 50 days of planting being slightly less than that in Comparative Example 1. This shows that the addition of Rhizomucor microphylla can improve the initial effect of the fertilizer, but will also cause the effect of the fertilizer to decrease in the middle and late stages of application.

[0085] By comparing Comparative Example 1 and Comparative Example 3, it can be seen that compared with Comparative Example 1, the self-made seaweed crushed material of the present invention is added to the base material of Comparative Example 3, as a result, the increase in fresh weight of the cabbage in the first 10 days and the second 10 days after planting is significantly reduced, and the increase in fresh weight in the third 10 days, the fourth 10 days and the fifth 10 days is increased; finally, the total increase in fresh weight of the cabbage in Comparative Example 3 after 50 days of planting is greater than that in Comparative Example 1. It shows that when the prepared fertilizer is applied to crops with a long growth cycle (50 days), the addition of seaweed crushed material can reduce the initial effect of the application of the prepared fertilizer, and at the same time can improve the effect of the fertilizer in the middle and late stages of application, and finally improve the overall effect of the fertilizer.

[0086] Combined with Example 1 for comparison, it can be seen that compared with Comparative Example 1, the homemade seaweed crushed materials of the present invention are added to the basic materials of Example 1, and Rhizomucor microphylla is added to the composite bacterial agent used for composting. As a result, the fresh weight increase of cabbage in the first 10 days, the second 10 days, the third 10 days, the fourth 10 days and the fifth 10 days after planting is increased; finally, the total amount of fresh weight increase of cabbage in Example 1 after 50 days of planting is significantly greater than that of Comparative Example 1, and at the same time, it is also significantly greater than that of Comparative Example 3. It shows that in the preparation of the fertilizer of the present invention, seaweed crushed materials and Rhizomucor microphylla are used together, and there is a synergistic effect between the two, which can synergistically improve the overall effect of the obtained fertilizer on long growth cycle crops (cabbage).

[0087] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0088] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A method for producing a biological organic fertilizer, characterized in that: The steps include: S1. Mix 0.8-1 parts of seaweed crushed material, 7.5-8 parts of straw and 1-1.3 parts of human and animal feces by weight to obtain a basic material; S2. Add a composite bacterial agent to the basic material obtained in S1, wherein the mass of the composite bacterial agent is 0.2-0.3% of the mass of the basic material, mix well, ventilate at a constant speed, and compost for 25-28 days to obtain a biological organic fertilizer; The composite bacterial agent comprises Bacillus licheniformis, nitrogen-fixing bacteria and Rhizomucor microplus; The production method of the seaweed crushed material is as follows: S11, drying the seaweed to a water content of 55-60%, and then chopping the seaweed to obtain shredded material; S12, adding sodium alginate to the crushed material obtained in S11, wherein the mass of sodium alginate is 7-9% of the mass of the crushed material, and mixing well to obtain seaweed crushed material.

2. The method for producing bio-organic fertilizer according to claim 1, characterized in that: In S1, 0.9 parts of seaweed crushed material, 7.8 parts of straw and 1.1 parts of human and animal feces are mixed uniformly by weight to obtain a basic material.

3. The method for producing bio-organic fertilizer according to claim 1, characterized in that: The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8×10 6 -1×10 7 CFU / g, the live bacteria content of nitrogen-fixing bacteria is 5×10 6 -7×10 6 CFU / g, the live bacteria content of Rhizomucor microplus is 1×10 6 -3×10 6 CFU / g.

4. The method for producing bio-organic fertilizer according to claim 3, characterized in that: The content of live bacteria of Bacillus licheniformis in the composite bacterial agent is 8.7×10 6 CFU / g, the content of live nitrogen-fixing bacteria was 5.9×10 6 CFU / g, the live bacteria content of Rhizomucor microplus was 2.2×10 6 CFU / g.

5. The method for producing bio-organic fertilizer according to claim 1, characterized in that: In S2, the specific operation of uniform ventilation is: ventilation for 12-15 minutes every hour, and the ventilation volume is 0.32-0.35L / (h·kg).

6. A biological organic fertilizer, characterized in that: The method is prepared by the production method according to any one of claims 1 to 5.

7. An application of a biological organic fertilizer, characterized in that: The bio-organic fertilizer is prepared by the production method according to any one of claims 1 to 5, and is suitable for short-growth-cycle vegetables and long-growth-cycle vegetables; the short-growth-cycle vegetables include water spinach, Chinese cabbage, lettuce, romaine lettuce, spinach, and rapeseed; the long-growth-cycle vegetables include cabbage, radish, cucumber, and tomato.

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