Compound fresh bamboo traditional Chinese medicine composition for relieving cough and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicines, and provides a compound fresh bamboo traditional Chinese medicine composition for relieving cough and application thereof, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines: 15-20 g of fresh bamboo, 15-20 g of astragalus membranaceus, 8-10 g of cassia twig, 6-10 g of trichosanthes kirilowii maxim, 6-10 g of bulbus fritillariae cirrhosae, 6-10 g of raw pinellia ternate, 3-5 g of radix peucedani, 3-5 g of white mulberry root-bark, 3-5 g of liquorice and 3-5 g of platycodon grandiflorum. The traditional Chinese medicine preparation disclosed by the invention has an all-around conditioning effect on different types of coughs through various mechanisms of clearing heat, moistening lung, warming yang, reducing phlegm and the like on the whole, and is particularly suitable for patients with coughs caused by deficiency-cold in spleen and stomach with phlegm heat; the traditional Chinese medicinal materials are matched for use, so that the weight of mice with deficiency-cold in spleen and stomach can be remarkably improved, the cough latency period of the mice with deficiency-cold in spleen and stomach is remarkably prolonged, the cough frequency of the mice is reduced, and a remarkable cough-relieving effect is achieved; meanwhile, the tracheal phenol red excretion amount of mice with deficiency-cold in spleen and stomach can be remarkably increased, and a remarkable phlegm reducing effect is achieved.

Description

Technical Field

[0001] The invention belongs to the field of Chinese medicine preparations, and particularly relates to a compound fresh bamboo Chinese medicine composition for relieving cough and application thereof. Background Art

[0002] Cough is one of the common symptoms of the respiratory system, usually caused by exogenous wind-cold, wind-heat, internal injury, phlegm and dampness and other factors. Traditional Chinese medicine has a long history in the field of cough and phlegm, and achieves antitussive effects in various ways, such as clearing heat and resolving phlegm, warming the middle and dispersing cold, nourishing yin and moistening the lungs and other different mechanisms of action. Commonly used Chinese medicine single prescriptions and compound prescriptions have achieved good results in the treatment of cough, such as single herbs such as Fritillaria cirrhosa and licorice, and compound prescriptions such as Qingfei Zhike Decoction and Maimendong Decoction. These medicines can relieve cough symptoms by regulating lung function and resolving phlegm, heat and dampness. With the continuous changes in clinical needs, compound Chinese medicines have received more and more attention, and better therapeutic effects can be achieved through the compatibility of multiple drugs.

[0003] In recent years, compound Chinese medicines based on bamboo extracts (such as fresh bamboo, bamboo shavings, etc.) have made great research progress in relieving cough and reducing phlegm. Traditional Chinese medicines with fresh bamboo as the main ingredient have the effects of clearing heat and moistening the lungs, reducing phlegm and relieving cough, and are suitable for patients with phlegm-heat cough. Through reasonable compatibility with other Chinese medicines such as Astragalus, Fritillaria cirrhosa, and Trichosanthes kirilowii, the comprehensive efficacy of the drug can be enhanced and adapted to a wider range of patient groups. The bamboo shavings extract in the compound fresh bamboo preparation has a unique effect of regulating qi and reducing phlegm, and is especially suitable for symptoms of hot cough and dry throat. The synergistic effect of different drug ingredients helps to comprehensively regulate the body and solve the problem of insufficient efficacy of a single drug.

[0004] The compound fresh bamboo Chinese medicine composition has multiple advantages in relieving cough: first, it can comprehensively regulate the respiratory function through various mechanisms (such as clearing heat and resolving phlegm, warming yang and dispersing cold, nourishing yin and moistening the lungs), and provide effective treatment for different types of coughs. Secondly, the compound medicine reduces the side effects that may be caused by the use of a single medicinal material through the synergistic effect of each medicinal material, and has better safety. Finally, fresh bamboo and its related extracts have a strong effect of clearing heat and moisturizing dryness, and are particularly suitable for hot coughs. However, the composition also has certain defects. For example, fresh bamboo is cold in nature and is not suitable for patients with spleen and stomach deficiency. In addition, the composition of the compound is complex, and long-term use may have an adverse effect on the spleen and stomach function, so it needs to be personalized according to the patient's constitution. Summary of the invention

[0005] In order to solve the above problems, the present invention firstly provides a compound fresh bamboo traditional Chinese medicine composition for relieving cough and its application.

[0006] The invention provides a composite fresh bamboo traditional Chinese medicine composition for relieving cough, which is prepared from the following raw materials by weight: 15-20g of fresh bamboo, 15-20g of astragalus, 8-10g of cassia twig, 6-10g of trichosanthes, 6-10g of fritillaria, 6-10g of raw pinellia, 3-5g of peucedanum, 3-5g of white mulberry bark, 3-5g of liquorice and 3-5g of platycodon.

[0007] In some embodiments, the Chinese medicine composition is prepared from the following raw materials by weight: 15g fresh bamboo, 15g astragalus, 10g cinnamon twig, 8g trichosanthes, 10g fritillaria, 10g raw pinellia, 5g peucedanum, 5g mulberry bark, 3g licorice, and 3g platycodon.

[0008] In some embodiments, the Chinese medicine composition is prepared from the following raw materials by weight: 20g fresh bamboo, 15g astragalus, 8g cinnamon twig, 6g trichosanthes, 8g fritillaria, 8g raw pinellia, 5g peucedanum, 3g mulberry bark, 3g licorice, and 5g platycodon.

[0009] In some embodiments, the Chinese medicine composition is prepared from the following raw materials by weight: 20g fresh bamboo, 20g astragalus, 8g cinnamon twig, 8g trichosanthes, 8g fritillaria, 8g cirrhosa, 6g raw pinellia, 3g peucedanum, 3g mulberry bark, 3g licorice, and 3g platycodon.

[0010] In some embodiments, the Chinese medicine composition is prepared from the following raw materials by weight: 15g fresh bamboo, 15g astragalus, 8g cinnamon twig, 8g trichosanthes, 6g fritillaria, 8g raw pinellia, 5g peucedanum, 5g mulberry bark, 3g licorice, and 3g platycodon.

[0011] In some embodiments, the Chinese medicine composition is prepared from the following raw materials by weight: 20g fresh bamboo, 15g astragalus, 10g cinnamon twig, 10g trichosanthes, 8g fritillaria, 10g raw pinellia, 5g peucedanum, 5g mulberry bark, 5g licorice, and 3g platycodon.

[0012] In addition, the present invention also provides an application of a traditional Chinese medicine preparation in preparing a medicine for treating cough.

[0013] The Chinese medicinal materials of the present invention have a unique mechanism of action in relieving cough, forming a prescription with comprehensive therapeutic effects, which mainly relieves cough symptoms through the multiple effects of clearing away heat and resolving phlegm, warming the middle and dispersing cold, invigorating qi and strengthening the body, and promoting lung function and relieving cough.

[0014] Main medicine: fresh bamboo, astragalus

[0015] Fresh bamboo: clears heat and resolves phlegm, moistens the lungs and relieves cough. It is mainly used to treat phlegm-heat blocking the lungs type cough, by clearing lung heat, diluting phlegm, reducing the sticky feeling of the respiratory tract, thus relieving cough.

[0016] Astragalus: replenishes Qi and strengthens the spleen, strengthens the body and eliminates pathogenic factors. Astragalus enhances the body's immune function, especially for coughs caused by Qi deficiency. It helps to clear phlegm and dampness and improve cough symptoms by enhancing the transport and transformation functions of the lungs and spleen. Astragalus also has the effect of strengthening the body's constitution, making the body more able to fight against external pathogens during treatment.

[0017] Assistant drugs: cinnamon twig, trichosanthes root, fritillaria cirrhosa, raw pinellia

[0018] Cinnamon twig: warms the meridians and dispels cold, promotes yang and transforms qi. Cinnamon twig has the effect of warming the lungs and dispelling cold, and is suitable for cold coughs or phlegm-damp coughs caused by cold pathogens. It warms the qi machine, promotes the release of lung qi, and alleviates the symptoms of cold coughs.

[0019] Trichosanthes: Clears away heat and resolves phlegm, moistens dryness and relieves chest tightness. Trichosanthes clears away hot and dry phlegm, unclogs the airways and helps with expectoration. It is particularly suitable for chest tightness and cough symptoms caused by phlegm heat.

[0020] Fritillaria cirrhosa: Clears heat and moistens the lungs, resolves phlegm and relieves cough. It has a good therapeutic effect on patients with fever cough, dry cough with little phlegm or sticky phlegm that is difficult to cough up. The lung-moistening effect of Fritillaria cirrhosa can relieve dry throat and reduce the frequency of coughing.

[0021] Raw Pinellia: Drying dampness and resolving phlegm, relieving adverse reactions and relieving cough. Pinellia has a strong dissolving effect on thick phlegm, especially for cough caused by damp phlegm blocking the lungs, by reducing mucus in the trachea and reducing cough reactions.

[0022] Adjuvant: Peucedanum chinense, Morus alba bark

[0023] Peucedanum: It can lower Qi and resolve phlegm, clear the lungs and relieve cough. The effect of Peucedanum is mainly reflected in its ability to lower Qi and resolve phlegm, making it easier to discharge phlegm in the airway. It is suitable for symptoms of excessive phlegm and frequent cough.

[0024] Mulberry bark: purging the lungs and calming asthma, clearing away heat and resolving phlegm. Mulberry bark is mainly used to treat lung heat, cough and asthma. Its lung purging effect can quickly reduce lung heat and help relieve cough, phlegm, asthma and other symptoms caused by lung heat.

[0025] Adjuvant: Licorice, Platycodon grandiflorum

[0026] Licorice: harmonizes all medicines, moistens the lungs and relieves coughs. Licorice is often used as a guiding drug to harmonize the properties of the medicines, and has certain effects of moistening the lungs, relieving coughs, and relieving urgency. Licorice can also reduce the irritation of the medicine to the stomach in traditional Chinese medicine prescriptions and improve the overall efficacy of the medicine.

[0027] Platycodon grandiflorum: It can clear the lungs and relieve throat pain, and remove phlegm and pus. Platycodon grandiflorum is a guiding drug that can guide the effects of other drugs directly to the lungs, while helping to remove phlegm and relieve throat discomfort.

[0028] This prescription plays a comprehensive conditioning role for different types of coughs (such as cold, hot, and phlegm-damp coughs) through multiple mechanisms such as clearing heat, moistening the lungs, warming the yang, and resolving phlegm. Fresh bamboo and astragalus as the main drugs play a basic role in clearing the lungs, resolving phlegm, and replenishing qi; the auxiliary drugs such as cinnamon twig and trichosanthes further enhance the therapeutic effect by warming the lungs and dispersing cold, clearing heat and moistening dryness; the auxiliary drugs Peucedanum chinense and Morus alba help to clear the lungs and expel phlegm, while licorice and platycodon play a role in harmonizing and guiding the efficacy of the medicine, so that the efficacy reaches the diseased area directly. This combination uses fresh bamboo and astragalus as the main drugs to play the role of clearing heat, moistening the lungs and replenishing qi, and cooperates with the auxiliary drugs Trichosanthes alba, Fritillaria cirrhosa, cinnamon twig, and raw pinellia to further clear heat and resolve phlegm and warm yang and dispel cold. The auxiliary drugs Peucedanum chinense and Morus alba help to descend qi and resolve phlegm, assist in the conditioning of lung function, and finally licorice and platycodon harmonize the medicinal properties and guide the medicine upward. This combination is suitable for patients with coughs caused by spleen and stomach deficiency and cold with phlegm and heat.

[0029] In addition, the combined use of the above-mentioned Chinese medicinal materials in the present invention can significantly improve the body weight of mice with spleen and stomach deficiency and cold, significantly prolong the cough latency period of mice with spleen and stomach deficiency and cold, reduce the number of coughing times of mice, and have a significant antitussive effect; at the same time, it can significantly increase the tracheal phenol red excretion of mice with spleen and stomach deficiency and cold, and has a significant expectorant effect. DETAILED DESCRIPTION

[0030] In order to make the technical problems to be solved, technical solutions and advantages of the present invention more clear, they are described in detail below in conjunction with specific embodiments.

[0031] Example 1

[0032] A composite fresh bamboo traditional Chinese medicine composition 1 for relieving cough is prepared from the following raw materials by weight:

[0033] 15g fresh bamboo, 15g astragalus, 10g cinnamon twig, 8g trichosanthes, 10g fritillaria, 10g raw pinellia, 5g peucedanum, 5g mulberry bark, 3g licorice, and 3g platycodon.

[0034] Example 2

[0035] A composite fresh bamboo traditional Chinese medicine composition 2 for relieving cough is prepared from the following raw materials by weight:

[0036] 20g fresh bamboo, 15g astragalus, 8g cinnamon twig, 6g trichosanthes, 8g fritillaria, 8g raw pinellia, 5g peucedanum, 3g mulberry bark, 3g licorice, and 5g platycodon.

[0037] Example 3

[0038] A composite fresh bamboo traditional Chinese medicine composition 3 for relieving cough is prepared from the following raw materials by weight:

[0039] 20g fresh bamboo, 20g astragalus, 8g cinnamon twig, 8g trichosanthes, 8g fritillaria, 8g cirrhosa, 6g raw pinellia, 3g peucedanum, 3g mulberry bark, 3g licorice, and 3g platycodon.

[0040] Example 4

[0041] A composite fresh bamboo traditional Chinese medicine composition 4 for relieving cough is prepared from the following raw materials by weight:

[0042] 15g fresh bamboo, 15g astragalus, 8g cinnamon twig, 8g trichosanthes, 6g fritillaria, 8g raw pinellia, 5g peucedanum, 5g mulberry bark, 3g licorice, and 3g platycodon.

[0043] Example 5

[0044] A composite fresh bamboo traditional Chinese medicine composition 5 for relieving cough is prepared from the following raw materials by weight:

[0045] 20g fresh bamboo, 15g astragalus, 10g cinnamon twig, 10g trichosanthes, 8g fritillaria, 10g raw pinellia, 5g peucedanum, 5g mulberry bark, 5g licorice, and 3g platycodon.

[0046] Example 6

[0047] A preparation method of a composite fresh bamboo Chinese medicine composition for relieving cough comprises the following steps: taking fresh bamboo, astragalus, cinnamon twig, trichosanthes, fritillaria cirrhosa, raw pinellia, peucedanum, white mulberry bark, liquorice and platycodon, weighing the dosage according to the formula of any one of Examples 1-5, and crushing all the medicinal materials into coarse powder, usually about 80 meshes. Soaking the coarsely crushed medicinal materials in 8 times the amount of water (i.e., 8 times the total weight of the medicinal materials), standing for 30 minutes to allow the medicinal materials to fully absorb water, and then decocting with warm water, decocting twice. After boiling with water for the first time, decocting with low heat for 40 minutes; adding 6 times the amount of water for the second time, decocting for 30 minutes, combining the two decoctions, filtering to remove the medicinal residues, and obtaining a water extract. Then, adding 50% ethanol to the remaining medicinal residues, soaking in a volume of 5 times the weight of the medicinal materials, and soaking for 24 hours. After soaking, ethanol reflux extraction is performed: reflux in a water bath of about 60°C for 2 hours, keeping a constant temperature during the reflux process, and collecting the extract. After the reflux extraction is completed, a secondary extraction is performed: add the same volume of ethanol, reflux for 1 hour, and collect the two ethanol extracts. The water extract and the ethanol extract are respectively concentrated under reduced pressure, and the excess solvent is removed to obtain a relatively concentrated extract. The concentrated water extract and the ethanol extract are combined and mixed to obtain 200 mL of concentrated Chinese medicine compound extract.

[0048] Example 7 Pharmacological Animal Experiment

[0049] Construction of a mouse model of spleen and stomach deficiency and cold: Male ICR mice were exposed to a low temperature environment (usually around 4°C) for 2-3 hours a day for 1-2 weeks to simulate the damage of the spleen and stomach in the cold environment, thereby causing physical weakness in the mice. The weight of the mice decreased significantly in the state of spleen and stomach deficiency and cold, and they also showed physical weakness, loss of appetite, and reduced food intake, indicating that the mouse model of spleen and stomach deficiency and cold was successfully constructed.

[0050] Effect of the compound fresh bamboo Chinese medicine composition on the body weight of mice with spleen and stomach deficiency and coldness: 70 male ICR mice with spleen and stomach deficiency and coldness were successfully constructed and divided into Chinese medicine group 1 (200mL / kg), Chinese medicine group 2 (200mL / kg), Chinese medicine group 3 (200mL / kg), Chinese medicine group 4 (200mL / kg), Chinese medicine group 5 (200mL / kg), and model group (200mL / kg normal saline). The normal group (200mL / kg normal saline) was healthy male ICR mice, with 10 mice in each group. Each drug-treated group was gavaged once a day for 28 consecutive days, and the changes in the initial and final body weight of the mice were recorded, as shown in Table 1.

[0051] Table 1 Effect of the compound fresh bamboo Chinese medicine composition on the body weight of mice with spleen and stomach deficiency (n=10)

[0052]

[0053]

[0054] Note: Compared with the normal group, a P<0.05, b P<0.01.

[0055] The results in Table 1 show that compared with the normal group, the body weight growth rate of mice in the model group was significantly reduced (P < 0.05); compared with the model group, the body weight growth rate of mice in Chinese medicine group 1, Chinese medicine group 2, Chinese medicine group 3, Chinese medicine group 4 and Chinese medicine group 5 were significantly increased (P < 0.05 or P < 0.01), which indicates that the compound fresh bamboo Chinese medicine composition of the present invention has the effect of significantly improving the body weight of mice with spleen and stomach deficiency and cold.

[0056] Construction of cough model in mice with spleen and stomach deficiency and coldness: 80 male ICR mice with spleen and stomach deficiency and coldness were successfully divided into pentoxyverine group (15.0 mg / kg), Guilong Kechuanning group (0.68 g / kg), Chinese medicine group 1 (200 mL / kg), Chinese medicine group 2 (200 mL / kg), Chinese medicine group 3 (200 mL / kg), Chinese medicine group 4 (200 mL / kg), Chinese medicine group 5 (200 mL / kg), and blank group (200 mL / kg normal saline), with 10 mice in each group. Each medication group was gavaged once a day for 28 consecutive days. One hour after the last medication, a 500 mL wide-mouth bottle was turned upside down on the operating table, and a ball of cotton with 0.3 mL concentrated ammonia was placed inside. The cotton was taken out after evaporation for 20 seconds, and the mice were quickly placed in the wide-mouth bottle. The cough latency of the mice and the number of coughs within 2 minutes were observed and recorded, as shown in Table 2.

[0057] Table 2 Effect of the compound fresh bamboo Chinese medicine composition on the cough latency and cough frequency of mice with spleen and stomach deficiency (n=10)

[0058]

[0059]

[0060] Note: Compared with the blank group, a P<0.05, b P<0.01.

[0061] The results in Table 2 show that compared with the blank group, the cough latency in the pentoxyverine group, Guilong Kechuanning group, and Chinese medicine group 1 to Chinese medicine group 5 was significantly prolonged (P < 0.05 or P < 0.01); the number of coughs in mice in each medication group was significantly reduced compared with the blank group (P < 0.05 or P < 0.01), which indicates that the compound fresh bamboo Chinese medicine composition of the present invention significantly prolongs the cough latency in mice with spleen and stomach deficiency and cold, reduces the number of coughs in mice, and has a significant antitussive effect.

[0062] Construction of tracheal phenol red excretion model in weak mice: 80 male ICR mice with spleen and stomach deficiency were successfully divided into bromhexine group (15.0 mg / kg), Guilong Kechuanning group (0.68 g / kg), Chinese medicine group 1 (200 mL / kg), Chinese medicine group 2 (200 mL / kg), Chinese medicine group 3 (200 mL / kg), Chinese medicine group 4 (200 mL / kg), Chinese medicine group 5 (200 mL / kg), and blank group (200 mL / kg normal saline), with 10 mice in each group. Each medication group was gavage-administered once a day for 28 consecutive days. One hour after the last medication, the mice were intraperitoneally injected with 20 mL / kg of 5% phenol red saline solution. After 30 minutes, the trachea was removed and placed in 4 mL of normal saline, and 0.2 mL of 5% sodium bicarbonate solution was added and shaken to completely release the phenol red in the trachea. The OD value was measured at 546 nm, and the excretion amount of phenol red was calculated according to the standard curve of phenol red, as shown in Table 3.

[0063] Table 3 Effect of the fresh bamboo traditional Chinese medicine composition on tracheal phenol red secretion in mice with spleen and stomach deficiency (n=10)

[0064] Group Phenol red secretion / (ng / mL) Blank group 1.23±0.36 Bromhexine group <![CDATA[2.45±0.64 a ]]> Guilong Kechuanning Group <![CDATA[2.56±0.45 a ]]> Chinese medicine group 1 <![CDATA[3.14±0.62 b ]]> Chinese medicine group 2 <![CDATA[3.45±0.47 a ]]> Chinese medicine group 3 <![CDATA[3.72±0.52 b ]]> Chinese medicine 4 groups <![CDATA[3.63±0.37 a ]]> Chinese medicine 5 groups <![CDATA[3.27±0.46 a ]]>

[0065] Note: Compared with the blank group, a P<0.05, b P<0.01.

[0066] The results in Table 3 show that compared with the blank group, the phenol red excretion of mice in the bromhexine group, the Guilong Kechuanning group, and the Chinese medicine group 1 to the Chinese medicine group 5 was significantly increased (P < 0.05 or P < 0.01), which indicates that the compound fresh bamboo Chinese medicine composition of the present invention can significantly increase the phenol red excretion of the trachea of ​​mice with spleen and stomach deficiency and cold, and has a significant expectorant effect.

[0067] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite fresh bamboo traditional Chinese medicine composition for relieving cough, characterized in that the composition is prepared from the following raw materials by weight: 15-20g fresh bamboo, 15-20g astragalus, 8-10g cassia twig, 6-10g trichosanthes, 6-10g fritillaria, 6-10g cirrhosa, 6-10g raw pinellia, 3-5g peucedanum, 3-5g mulberry bark, 3-5g liquorice, and 3-5g platycodon.

2. The Astragalus Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition is prepared from the following raw materials by weight: 15g fresh bamboo, 15g astragalus, 10g cinnamon twig, 8g trichosanthes, 10g fritillaria, 10g raw pinellia, 5g peucedanum, 5g mulberry bark, 3g liquorice and 3g platycodon.

3. The Astragalus Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following raw materials by weight: 20g fresh bamboo, 15g astragalus, 8g cinnamon twig, 6g trichosanthes, 8g fritillaria, 8g raw pinellia, 5g peucedanum, 3g mulberry bark, 3g liquorice, and 5g platycodon.

4. The Astragalus Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following raw materials by weight: 20g fresh bamboo, 20g astragalus, 8g cinnamon twig, 8g trichosanthes, 8g fritillaria, 6g raw pinellia, 3g peucedanum, 3g mulberry bark, 3g liquorice, and 3g platycodon.

5. The Astragalus Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following raw materials by weight: 15g fresh bamboo, 15g astragalus, 8g cinnamon twig, 8g trichosanthes, 6g fritillaria, 8g raw pinellia, 5g peucedanum, 5g mulberry bark, 3g liquorice, and 3g platycodon.

6. The Astragalus Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is prepared from the following raw materials by weight: 20g fresh bamboo, 15g astragalus, 10g cinnamon twig, 10g trichosanthes, 8g fritillaria, 10g raw pinellia, 5g peucedanum, 5g mulberry bark, 5g liquorice and 3g platycodon.

7. Use of the Chinese medicine composition according to any one of claims 1 to 6 in the preparation of a medicament for treating cough.

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