Automatic magnetic bead cleaning and separating device

Abstract

The invention discloses an automatic magnetic bead cleaning and separating device and aims to solve the problems that in the prior art, in order to complete the cleaning process of whole magnetic beads and reaction cups, the number of modules is large, part of the modules are large, the structure is very complex after series connection use, and the cleaning efficiency is low. The technical problem is solved through the following technical scheme: the cleaning device comprises a magnetic disk turntable which is provided with a magnetic disk rotating shaft and a cleaning hole position rotating around the magnetic disk rotating shaft; the cleaning device is arranged above the magnetic disk turntable; the cleaning device comprises a liquid injection disc and a liquid suction disc which are arranged in the vertical direction, and the liquid suction disc is arranged above the liquid injection disc. Lifting devices for controlling the liquid injection disc and the liquid suction disc to independently lift are arranged on the liquid injection disc and the liquid suction disc; a liquid injection device is arranged on the liquid injection disc and injects liquid into the reaction cup; a liquid suction device is arranged on the liquid suction disc, and liquid in the reaction cup is sucked out by the liquid suction device; and the uniform mixing device is used for uniformly mixing the liquid injected by the liquid injection device in the reaction cup.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and more specifically, to an automatic magnetic bead cleaning and separation device. Background Art

[0002] In the field of in vitro diagnosis, the immune complex marker will bind to the magnetic beads after the sample is added, but the bound magnetic beads are still mixed with other unbound markers in the reagent. Therefore, a separation and cleaning device is needed to separate the magnetic beads and clean up the remaining unnecessary substances. The accuracy of the reading can only be guaranteed after thorough cleaning. The cleaning and separation of the magnetic beads requires incubation in advance to allow the marker to fully bind to the magnetic beads, and then multi-stage cleaning is performed on them. After the cleaning is completed, a medium is added to allow it to emit light and read the value. In the prior art, in the above-mentioned entire process, many devices and modules are needed, and some modules are relatively large. When they are used in series, the structure is complicated and the cleaning efficiency is not high enough. Summary of the invention

[0003] The present invention overcomes the problems in the prior art that, in order to complete the entire cleaning process of magnetic beads and reaction cups, a large number of modules are required, some modules are relatively large, and the structure is very complicated after being used in series, resulting in low cleaning efficiency. An automatic magnetic bead cleaning and separation device is provided, which can automatically clean the magnetic beads, and the relevant modules for cleaning the magnetic beads are all arranged above the magnetic disk turntable, and has the characteristics of compact structure, high degree of automation and high cleaning efficiency.

[0004] In order to solve the above technical problems, the present invention adopts the following technical solution: an automatic magnetic bead cleaning and separation device, comprising: The disk turntable is provided with a disk shaft and a cleaning hole rotating around the disk shaft; The cleaning device is arranged above the magnetic disk turntable; the cleaning device comprises a liquid injection plate and a liquid suction plate arranged in a vertical direction, the liquid suction plate is arranged above the liquid injection plate; the liquid injection plate and the liquid suction plate are both provided with a lifting device for controlling their independent lifting; the liquid injection plate is provided with a liquid injection device, the liquid injection device injects liquid into the reaction cup; the liquid suction plate is provided with a liquid suction device, the liquid suction device sucks out the liquid in the reaction cup; The mixing device mixes the liquid in the reaction cup after the liquid is injected by the injection device.

[0005] In the present application, the liquid injection plate and the liquid suction plate are arranged above the magnetic disk turntable, and then the liquid suction device, the liquid injection device, the mixing device and other structures are arranged on the liquid injection plate, so that the overall structure is more compact, which greatly saves the equipment space. At the same time, each device can cooperate with each other to perform the steps of liquid injection, mixing and cleaning on the reaction cup in sequence, reducing unnecessary reaction cup transfer processes, thereby improving the efficiency of reaction cup and magnetic bead cleaning, and improving the efficiency of the experiment.

[0006] Preferably, the liquid absorbing device comprises a liquid absorbing pad block arranged on the liquid absorbing plate, a liquid absorbing needle penetrating through and slidably arranged in the liquid absorbing pad block, and a first buffer structure is arranged on the top of the liquid absorbing needle.

[0007] The aspiration needle is driven to move up and down by the control of the aspiration plate, so that the aspiration needle can suck the liquid in the reaction cup cleanly.

[0008] Preferably, the injection device comprises a bracket arranged on the injection tray, a nut column is arranged on the top of the bracket, an injection needle is penetrated and slidably arranged in the nut column, and a second buffer structure is arranged on the top of the injection needle.

[0009] The injection needle is driven to move up and down by the control of the injection plate, so that the injection needle can inject liquid into the reaction cup.

[0010] Preferably, the mixing device includes a clamping device for gripping the reaction cup and a power device for driving the clamping device to rotate; the clamping device includes a rotating cup-grabbing shaft rotatably arranged on the pipette plate, a rotating synchronous wheel arranged on the rotating cup-grabbing shaft and an elastic gripping clamp arranged below the rotating cup-grabbing shaft; a top shaft is arranged through the center of the rotating cup, and an injection needle is arranged through the top shaft; upper and lower thrust blocks are arranged at the upper end of the top shaft, and the upper and lower thrust blocks are located below the pipette plate; a third buffer structure is arranged between the upper and lower thrust blocks and the rotating synchronous wheel.

[0011] Through the above structure, the liquid injection device is combined with the mixing device, so that the transfer step between liquid injection and mixing is omitted, which not only makes the overall structure more compact, but also improves the cleaning efficiency of the reaction cup and magnetic beads, thereby improving the efficiency of the experiment.

[0012] Preferably, the third buffer structure is a thrust spring disposed between the upper and lower thrust blocks and the rotating synchronous wheel.

[0013] The thrust spring can provide support force for the upper and lower thrust blocks, and can also provide restoring force for the upper and lower thrust blocks, so that when the reaction cup is ejected from the elastic gripping clamp by the ejection shaft, the upper and lower thrust blocks can automatically reset.

[0014] Preferably, the liquid injection device is provided with several groups; the power device comprises a synchronous belt which is in contact with the rotating synchronous wheel, and a power driving device which drives the synchronous belt to rotate.

[0015] The power drive device drives the synchronous belt, and the synchronous belt can drive multiple tension wheels, driving wheels, and synchronous wheels at the same time.

[0016] Preferably, the power device further comprises a plurality of tensioning wheels in contact with the synchronous belt; among the plurality of tensioning wheels, at least one sliding tensioning wheel is provided.

[0017] The sliding tensioner can continuously provide tension to the synchronous belt, thereby preventing the synchronous belt from slipping during transmission.

[0018] Preferably, a driving assembly for driving the disk shaft to rotate is provided at the bottom thereof, and the driving assembly includes a shaft driven wheel fixedly connected to the disk shaft and a shaft power device for driving the shaft driven wheel to rotate; a shaft driving wheel is provided on the output shaft of the shaft power device, and the shaft driving wheel and the shaft driven wheel are linked by a synchronous belt.

[0019] The rotating shaft driving wheel and the rotating shaft driven wheel are linked by a synchronous belt. The angle of the disk rotating shaft is controlled by controlling the rotation angle of the stepper motor, thereby controlling the rotation angle of the incubation turntable.

[0020] Preferably, a code tooth piece is provided at the bottom of the disk shaft, and a reset photoelectric switch and a rotation photoelectric switch corresponding thereto are provided at the edge of the code tooth piece; the reset photoelectric switch controls the reset of the disk turntable; and the rotation photoelectric switch controls the rotation amount of the disk turntable.

[0021] The reset photoelectric switch controls the reset of the disk turntable; the rotation photoelectric switch controls the rotation amount of the disk turntable. It can accurately control the rotation angle of the disk shaft and control the reset of the disk shaft.

[0022] Preferably, a reading device is arranged on the side wall of the magnetic disk turntable, and the reading device comprises a reading support and a reader arranged in the reading support, and the reader reads the light value in the reaction cup.

[0023] The reader can perform a collective interpretation of the light value emitted when the immune conjugate in the reaction cup reacts with the excitation liquid, and materialize the luminescence amount into a numerical value to facilitate subsequent experimental calculations and other steps.

[0024] Compared with the prior art, the present invention has the following beneficial effects: The present invention arranges the liquid injection plate and the liquid suction plate above the magnetic disk turntable, and then arranges the liquid suction device, the liquid injection device, the mixing device and other structures on the liquid injection plate, so that the overall structure is more compact and the equipment space is greatly saved. At the same time, the various devices can cooperate with each other to perform the steps of liquid injection, mixing and cleaning on the reaction cup in sequence, reducing unnecessary reaction cup transfer processes, thereby improving the efficiency of reaction cup and magnetic bead cleaning and improving the efficiency of the experiment. In addition, the present application creatively combines the liquid injection device with the mixing device, so that the transportation process of the reaction cup between liquid injection and mixing is omitted, which not only makes the overall structure more compact, but also improves the cleaning efficiency of the reaction cup and magnetic beads, thereby improving the efficiency of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a three-dimensional diagram of the overall structure of the present invention.

[0026] Figure 2 It is a top view of the overall structure of the present invention.

[0027] Figure 3 It is a three-dimensional structural diagram of the magnetic disk turntable of the present invention.

[0028] Figure 4 It is a three-dimensional structural diagram of the disk turntable of the present invention from another angle.

[0029] Figure 5 It is a top view of the magnetic disk turntable of the present invention.

[0030] Figure 6 yes Figure 5 Cross-sectional view along the AA direction.

[0031] Figure 7 It is a three-dimensional structural diagram of the cleaning device of the present invention.

[0032] Figure 8 It is a three-dimensional structural diagram of the cleaning device of the present invention from another angle.

[0033] Fig. 9 It is a schematic diagram of the layout structure of the power device of the present invention.

[0034] Fig.10 It is a cross-sectional view of the interpretation device of the present invention.

[0035] Fig.11 It is a front view of the liquid injection device of the present invention.

[0036] Fig.12 It is a cross-sectional view of the liquid injection device of the present invention and its related structures.

[0037] In the figure: 1, disk turntable, 11, fixed bottom plate, 111, bottom plate support column, 112, fixed block, 12, disk shaft, 13, heating plate, 131, heating plate pressure plate, 132, temperature sensor, 133, thermal protection switch, 14, incubation turntable, 141, incubation hole position, 142, cleaning hole position, 15, code tooth plate, 151, end cover, 152, reset photoelectric switch, 153, rotating photoelectric switch off, 16, shaft driven wheel, 17, shaft power device, 171, shaft driving wheel, 18, disk upper plate, 181, incubation placement hole, 182, cleaning placement hole, 183, washing liquid injection block, 184, excitation liquid A injection block, 185, excitation liquid B injection block, 186, guide rod, 19, reading device, 191, reading support, 192, reader, 193, circuit board, 194, cover; 2. Liquid injection device, 21. Liquid injection plate, 211. Liquid aspiration needle guide seat, 22. Bracket, 23. Liquid injection needle, 24. Liquid injection spring, 3. Liquid suction device, 31. Liquid suction plate, 32. Liquid suction pad, 33. Liquid suction needle, 4. Clamping device, 41. Rotating cup grabbing shaft, 42. Rotating synchronous wheel, 43. Elastic grabbing clamp, 44. Top shaft, 45. Upper and lower thrust blocks, 46. Thrust spring, 47. Flange bearing; 5. power device, 51. rotating motor, 53. rotating driven wheel, 54. first tensioning wheel, 55. second tensioning wheel; 6. Screw lifting device, 61. Screw motor, 62. Screw seat; 7. Synchronous belt lifting device, 71. Upper and lower load plates, 72. Lifting driven wheel, 73. Lifting synchronous wheel, 74. Guide rail, 75. Slider. DETAILED DESCRIPTION

[0038] The technical solution of the present invention is further described in detail below through specific embodiments and in conjunction with the accompanying drawings: Example 1: Reference Figures 1 to 12 As shown, an automatic magnetic bead cleaning and separation device comprises: a magnetic disk turntable 1, a cleaning device and a mixing device.

[0039] The disk turntable 1 is provided with a disk rotation shaft 12 and a cleaning hole 142 rotating around the disk rotation shaft 12 .

[0040] The cleaning device is arranged above the magnetic disk turntable 1; the cleaning device includes a liquid injection plate 21 and a liquid suction plate 31 arranged in a vertical direction, and the liquid suction plate 31 is arranged above the liquid injection plate 21; both the liquid injection plate 21 and the liquid suction plate 31 are provided with a lifting device for controlling their independent lifting; the liquid injection plate 21 is provided with a liquid injection device 2, and the liquid injection device 2 injects liquid into the reaction cup; the liquid suction plate 31 is provided with a liquid suction device 3, and the liquid suction device 3 sucks the liquid in the reaction cup.

[0041] The mixing device mixes the liquid in the reaction cup after the liquid is injected by the liquid injection device 2.

[0042] like Figure 3 and Figure 4As shown, the disk turntable 1 is arranged on a fixed bottom plate 11, and a plurality of bottom plate support columns 111 are arranged at the bottom of the fixed bottom plate 11 to support the fixed bottom plate 11. A fixed block 112 is arranged on the fixed bottom plate 11 to support the disk turntable 1, and the fixed block 112 is arranged along the circumferential direction, and the disk is mounted on the fixed block 112. A disk shaft 12 is arranged at the center of the disk turntable 1, and the disk shaft 12 is arranged in the disk turntable 1 through a bearing and a locking nut. A heating plate 13 is arranged below the disk turntable 1, and the heating plate 13 can provide heat for incubation. The heating plate 13 is fixed to the bottom of the disk turntable 1 through a heating plate pressing plate 131, and a temperature sensor 132 and a thermal protection switch 133 are arranged on the side of the heating plate 13. The temperature sensor 132 can detect the temperature in real time. When the temperature reaches a certain height, the thermal protection switch 133 works to prevent the temperature from being too high.

[0043] like Figures 3 to 6 As shown, an incubation turntable 14 is provided inside the disk turntable 1, and the incubation turntable 14 rotates with the rotation of the disk shaft 12. The incubation turntable 14 is divided into two circles, an inner circle and an outer circle. The inner circle in this embodiment is provided with 4 rows of holes, each row has 30 holes, a total of 120 holes, which are incubation holes 141, and the reaction cups after sample addition will be placed here for heating and incubation. 120 holes are sufficient to meet the needs of ordinary experiments and emergency needs. The outer circle is 30 cleaning holes 142. When the incubation time of the reaction cup is up, it will be transferred to the outer circle for the cleaning process. In order to ensure that the cleaning holes 142 are not light-transmitting, the outer circle holes are designed to have a darkroom effect. After the reaction cup is placed in, it sinks into the cleaning hole 142, thereby isolating the external light to the maximum extent.

[0044] A circle of magnets is arranged on the side wall of the disk turntable 1 in a NSNS... manner, thereby forming a magnetic field in the disk turntable 1. When the incubation turntable 14 rotates into the magnetic field, the strong magnetic force will adsorb the magnetic beads in the reaction cup to the wall of the reaction cup, thereby preventing the magnetic beads from being sucked away when the liquid in the reaction cup is sucked away.

[0045] like Figure 4 As shown, a code tooth piece 15 and a shaft driven wheel 16 are provided at the bottom of the disk shaft 12, and the shaft driven wheel 16 and the code tooth piece 15 are fixed to the bottom of the disk shaft 12 through an end cover 151. The edge of the code tooth piece 15 is provided with a reset photoelectric switch 152 and a rotation photoelectric switch 153 corresponding thereto; the reset photoelectric switch 152 controls the reset of the disk turntable 1; and the rotation photoelectric switch 153 controls the rotation amount of the disk turntable 1.

[0046] A driving assembly for driving the disk shaft 12 to rotate is provided at the bottom of the fixed base plate 11. The driving assembly includes a shaft driven wheel 16 fixedly connected to the disk shaft 12 and a shaft power device 17 for driving the shaft driven wheel 16 to rotate. The shaft power device 17 in this embodiment includes but is not limited to a stepper motor. The output shaft of the stepper motor is provided with a shaft driving wheel 171, and the shaft driving wheel 171 is linked to the shaft driven wheel 16 through a synchronous belt. The angle of the disk shaft 12 is controlled by controlling the rotation angle of the stepper motor, thereby controlling the rotation angle of the incubation turntable 14.

[0047] like Figure 4 As shown, a reading device 19 is provided on the side wall of the disk turntable 1, and the reading device 19 provides a reading function. Fig.10 As shown, the reading device 19 includes a reading support 191 and a reader 192 disposed in the reading support, the reader is connected to a circuit board 193, and a cover 194 is disposed outside the reading support. The reading support is fixedly disposed on the outer wall of the disk turntable 1, and the reader reads the light value in the reaction cup. The reader can perform a concentrated reading of the light value emitted when the immune conjugate in the reaction cup reacts with the excitation liquid, and materialize the luminescence amount into a numerical value to facilitate subsequent experimental calculations and other steps.

[0048] A disk upper plate 18 is disposed above the disk turntable 1, and a cleaning device is installed above the disk upper plate 18. Figure 2 As shown, a plurality of incubation placement holes 181 matching the incubation holes 141 are arranged on the disk upper plate 18; a cleaning placement hole 182 corresponding to the cleaning hole 142 is arranged in the circumferential direction of the disk upper plate 18, and the reaction cup is placed on the cleaning hole 142 through the cleaning placement hole 182. At the same time, a washing liquid injection block 183, an excitation liquid A injection block 184 and an excitation liquid B injection block 185 are also arranged on the disk upper plate 18.

[0049] The cleaning device is divided into two layers, the lower layer is the liquid injection plate 21, and the upper layer is the liquid suction plate 31. Figure 1 As shown, both the liquid injection plate 21 and the liquid suction plate 31 are provided with a lifting device capable of driving the liquid suction plate 31 to move in the vertical direction. In order to make the liquid suction plate 31 and the liquid injection plate 21 move more stably in the vertical direction, two guide rods 186 are provided on the magnetic disk upper plate 18, and guide holes cooperating with the guide rods 186 are provided on both sides of the liquid injection plate 21 and the liquid suction plate 31, and linear bearings are provided in the guide holes, and the guide rods 186 can slide freely in the linear bearings.

[0050] like Figure 1As shown, the liquid suction device 3 includes a liquid suction pad 32 arranged on the liquid suction disk 31, a liquid suction needle 33 penetrating and slidingly arranged in the liquid suction pad 32, and a first buffer structure is arranged on the top of the liquid suction needle 33. The liquid suction device 3 in this embodiment is provided with three groups, and the three groups of liquid suction devices 3 have the same structure. A liquid suction needle guide seat 211 corresponding to the liquid suction pad 32 is arranged on the liquid injection disk 21, and the liquid suction needle guide seat 211 is penetratingly arranged on the liquid injection disk 21. The upper end of the liquid suction needle 33 is arranged in the liquid suction pad 32 through a nut, and the lower end of the liquid suction needle 33 slides freely in the liquid suction needle guide seat 211. The first buffer structure in this application includes but is not limited to a liquid suction spring, and the two ends of the liquid suction spring are respectively abutted and connected with the nut and the liquid suction needle 33, and the liquid suction spring plays a buffering role for the liquid suction needle 33. A liquid suction hole corresponding to the liquid suction needle 33 is arranged on the disk upper disk 18, so that the liquid suction needle 33 can pass through the liquid suction hole to suck out the liquid in the reaction cup.

[0051] There are two groups of liquid injection devices 2, and the two groups of liquid injection devices 2 and the three groups of liquid suction devices 3 cooperate with each other. The two groups of liquid injection devices 2 are inserted between the three groups of liquid suction devices 3, thereby completing three-stage cleaning.

[0052] like Figure 1 , Fig.11 , Fig.12 As shown. The injection device 2 includes a bracket 22 arranged on the injection plate 21, the upper end of the bracket 22 passes over the suction plate 31, a nut column is arranged on the top of the bracket 22, an injection needle 23 is penetrated and slidably arranged in the nut column, and a second buffer structure is arranged on the top of the injection needle 23. (The connection structure between the top of the injection needle 23 and the bracket 22 is similar to the structure between the top of the suction needle 33 and the suction plate 31). The upper end of the injection needle 23 is arranged in the nut column through a nut. The second buffer structure in this embodiment includes but is not limited to the injection spring 24, the two ends of the injection spring 24 are respectively abutted against the nut column and the injection needle 23, and the injection spring 24 plays a buffering role for the injection needle 23. An injection hole corresponding to the injection needle 23 is arranged on the upper disk 18 of the magnetic disk, so that the injection needle 23 can pass through the injection hole to inject liquid in the reaction cup.

[0053] The mixing device includes a clamping device 4 for gripping the reaction cup and a power device 5 for driving the clamping device 4 to rotate. In the present application, in order to make the overall structure more compact and simpler, the clamping device 4 is combined with the liquid injection device 2 so that the reaction cup can be directly rotated after the liquid is injected, thereby improving the cleaning efficiency.

[0054] like Fig.11 , Fig.12As shown, the clamping device 4 includes a rotating cup grabbing shaft 41 rotatably arranged on the liquid suction disc 31, a rotating synchronous wheel 42 arranged on the rotating cup grabbing shaft 41, and an elastic gripper 43 arranged below the rotating cup grabbing shaft 41. A top shaft 44 is arranged through the center of the rotating cup grabbing shaft 41, and the injection needle 23 is arranged through the top shaft 44; upper and lower thrust blocks 45 are arranged at the upper end of the top shaft 44, and the upper and lower thrust blocks are located below the liquid suction disc 31; a third buffer structure is arranged between the upper and lower thrust blocks and the rotating synchronous wheel 42. The third buffer structure is a thrust spring 46 arranged between the upper and lower thrust blocks 45 and the rotating synchronous wheel 42. Two flange bearings 47 are arranged at positions corresponding to the liquid suction disc 31 and the liquid suction needle 33, and the rotating cup grabbing shaft 41 is arranged in the flange bearings 47, so that the rotating cup grabbing shaft 41 can rotate freely in the flange bearings 47.

[0055] like Fig. 9 As shown, the power device 5 includes a synchronous belt that fits the rotating synchronous wheel 42 and a power drive device that drives the synchronous belt to rotate. The power drive device in the present application includes but is not limited to a rotating motor 51, a rotating driving wheel is arranged on the output shaft of the rotating motor 51, and a rotating driven wheel 53 is arranged on the other end of the suction plate 31. A first tensioning wheel 54 is arranged between the rotating driven wheel 53 and the adjacent rotating synchronous wheel 42. In the present application, two groups of liquid injection devices 2 are arranged, so a second tensioning wheel 55 is arranged between the two rotating synchronous wheels 42. The synchronous belt is sequentially wound around the rotating driving wheel, the rotating driven wheel 53, the first tensioning wheel 54, the rotating synchronous wheel 42, the second tensioning wheel 55, and the rotating synchronous wheel 42, so that when the output shaft of the rotating motor 51 rotates, the rotating synchronous wheel 42 can be driven to rotate through the synchronous belt, thereby driving the rotating cup grabbing shaft 41 to rotate, and then driving the elastic grabbing clamp 43 at the bottom to rotate, so that after the reaction cup is filled with liquid, the elastic grabbing clamp 43 can rotate with the reaction cup, so that the reaction cup can be cleaned more cleanly.

[0056] In order to prevent the synchronous belt from slipping during transmission, the power device 5 is provided with a plurality of tensioning wheels that fit the synchronous belt; among the plurality of tensioning wheels, at least one sliding tensioning wheel is provided. Specifically, in this embodiment, the first tensioning wheel 54 can slide on the liquid injection plate 21, and the first tensioning wheel 54 can be continuously pushed by the spring, thereby providing a tensioning force for the synchronous belt to prevent the synchronous belt from slipping during transmission.

[0057] In the present application, a total of three aspiration needles 33 and two injection needles 23 are provided, and the two injection needles 23 are interspersed between the three aspiration needles 33, and the washing liquid injection block 183 is provided on the magnetic disk 18, so as to cooperate with the three-stage cleaning of the reaction cup and the magnetic beads. Of course, the number of the aspiration needles 33 and the injection needles 23 is not specifically limited, and the number of the aspiration needles 33 and the injection needles 23 can be set according to actual needs to complete the cleaning of different numbers of stages.

[0058] An excitation liquid A injection block 184 is provided on the left side of the disk upper disk 18, and the excitation liquid A can be added to the reaction cup after cleaning. And an excitation liquid B injection block 185 is also provided just above the reading device, and the excitation liquid B can be added thereto, so as to make a collective reading of the light value emitted when the immune conjugate in the reaction cup reacts with the excitation liquid, and materialize the luminescence amount into a numerical value for the convenience of subsequent experimental calculations and other steps.

[0059] The working principle of the present application is as follows: a reaction cup filled with a reagent is placed into the cleaning hole 142 from the cleaning placement hole 182, the incubation turntable 14 rotates, and the reaction cup comes to the cleaning solution injection block 183 to be filled with cleaning solution.

[0060] After the filling is completed, the incubation turntable 14 continues to rotate to the first aspiration needle 33. At this time, the lifting device that controls the up and down movement of the injection plate is started, and the lifting device lowers the height of the injection plate, and then the lifting device that controls the up and down movement of the aspiration plate 31 is started, so that the aspiration plate 31 moves downward until the aspiration needle 33 can reach the bottom of the reaction cup, and then the aspiration needle 33 starts to aspirate liquid. Because the magnetic disk turntable 1 is equipped with a magnet, all the magnetic beads in the reaction cup are adsorbed on the cup wall at this time, so the aspiration needle 33 will only aspirate the liquid, not the magnetic beads.

[0061] After the liquid aspiration is completed, the lifting device is started again, and the aspiration plate 31 and the injection plate are reset in turn through the induction of the photoelectric switch. At the same time, the aspiration spring and the injection spring 24 in the nut can also reset the aspiration needle 33 and the injection needle 23. After the end, the incubation turntable 14 continues to rotate and comes to the first injection needle 23. The same lifting device is started, and the injection plate descends, so that the rotating gripper completely covers the upper edge of the reaction cup. Then the lifting device drives the injection plate to rise and lifts the reaction cup to a certain height. The injection needle 23 injects a certain amount of liquid into the reaction cup. After that, the rotating motor 51 is started, and the rotating synchronous wheel 42 is driven to rotate through the synchronous belt, so that the rotating gripper rotates, and the reaction cup rotates with the rotating gripper, so that the liquid in the reaction cup is mixed.

[0062] After the mixing is completed, the injection plate first descends to a certain distance, and then the suction plate 31 descends. When the suction plate 31 descends, it presses the upper and lower thrust blocks 45 and descends together, so that the top shaft 44 descends together, until the lower end of the top shaft 44 pushes the reaction cup out of the rotating clamp. After the reaction cup falls into the cleaning hole 142, the suction plate 31 and the injection plate are reset in turn, and the thrust spring 46 rebounds at the same time, so that the upper and lower thrust blocks 45 and the injection needle 23 return to the starting position.

[0063] Then the incubation turntable 14 continues to rotate to the position of the second aspiration needle 33, repeats the above-mentioned aspiration action, and then rotates to the position of the injection needle 23, repeats the above-mentioned injection action, until three injections are completed. After three aspirations, the cleaning process is considered to be completed.

[0064] After the cleaning is completed, the incubation turntable 14 continues to rotate and comes to the position of the exciting liquid A injection block 184, and the exciting liquid A is added. After the filling is completed, the reaction cup is transferred to the mixing module by the robot for mixing, and then transferred to the specified position of the incubation turntable 14 by the robot. After that, the incubation turntable 14 continues to rotate and comes to the judgment position to add liquid B for judgment. After the judgment is completed, the incubation turntable 14 continues to rotate to the discard port, and the robot discards the reaction cup. In this way, the entire cleaning and judgment process is completed.

[0065] In the present application, by setting the liquid injection plate and the liquid suction plate 31 above the magnetic disk turntable 1, and then arranging the liquid suction device 3, the liquid injection device, the mixing device and other structures, all on the liquid injection plate, the overall structure is more compact, and the equipment space is greatly saved. At the same time, each device can cooperate with each other to perform the steps of liquid injection, mixing and cleaning on the reaction cup in sequence, reducing unnecessary reaction cup transfer processes, thereby improving the efficiency of reaction cup and magnetic bead cleaning, and improving the efficiency of the experiment. In addition, the present application creatively combines the liquid injection device with the mixing device, so that the step of reaction cup transportation between liquid injection and mixing is omitted, which not only makes the overall structure more compact, but also improves the cleaning efficiency of the reaction cup and magnetic beads, thereby improving the efficiency of the experiment.

[0066] Example 2: Reference Figure 7 As shown, the structure of this embodiment is similar to that of Embodiment 1, except that the lifting device on the liquid injection plate 21 is a screw lifting device 6, and the lifting device on the liquid suction plate 31 is a synchronous belt lifting device 7.

[0067] The screw lifting device 6 includes a screw motor 61 fixedly arranged on the liquid injection tray, a screw seat 62 is arranged on the magnetic disk upper plate 18, one end of the screw is inserted into the screw motor 61, and the other end of the screw is fixedly connected to the screw seat 62. When the screw motor 61 rotates, the screw motor 61 moves up and down along the screw, thereby driving the liquid injection tray to move up and down.

[0068] The synchronous belt lifting device 7 is fixedly arranged on the outer side. The synchronous belt lifting device 7 includes an upper and lower download plate 71, which is fixedly installed on the magnetic disk upper plate 18 through a mounting block. A lifting driven wheel 72 and a lifting synchronous wheel 73 are arranged on the upper and lower download plate 71 in the vertical direction, and a lifting synchronous belt is wound around the lifting synchronous wheel 73 and the lifting driven wheel 72. A stepping motor for driving the lifting synchronous belt to rotate is arranged on the upper and lower download plate 71. A guide rail 74 is arranged on the upper and lower download plate 71, and a slider 75 that slides along the guide rail 74 is arranged on the liquid suction plate 31. The synchronous belt pressing plate presses the lifting synchronous belt against the slider 75, so that the lifting synchronous belt can drive the liquid suction plate 31 to move in the vertical direction.

[0069] In addition, a photoelectric gate switch is provided at the upper end of the upper and lower load plates 71, and a zero position piece cooperating with the photoelectric gate is provided on the slider 75. The zero position piece cooperates with the photoelectric gate switch to control the liquid suction plate 31 to reset.

[0070] In this embodiment, the screw lifting device 6 controls the lifting and lowering of the liquid injection plate 21 based on the principle of the ball screw, so that the displacement of the liquid injection plate 21 in the vertical direction can be accurately controlled, so that during the experiment, the distance between the liquid injection needle 23 and the reaction cup can be accurately controlled. The synchronous belt lifting device 7 also has the characteristics of high operating accuracy, so that the displacement of the liquid suction plate 31 in the vertical direction can be accurately controlled, and the distance between the liquid suction needle 33 and the reaction cup can be accurately controlled.

[0071] The above-described embodiments are only preferred solutions of the present invention and are not intended to limit the present invention in any form. Other variations and modifications are possible without exceeding the technical solutions described in the claims.

Claims

1. An automatic magnetic bead cleaning and separation device, characterized in that: include: The disk turntable is provided with a disk shaft and a cleaning hole rotating around the disk shaft; The cleaning device is arranged above the magnetic disk turntable; the cleaning device comprises a liquid injection plate and a liquid suction plate arranged in a vertical direction, the liquid suction plate is arranged above the liquid injection plate; the liquid injection plate and the liquid suction plate are both provided with a lifting device for controlling their independent lifting; the liquid injection plate is provided with a liquid injection device, the liquid injection device injects liquid into the reaction cup; the liquid suction plate is provided with a liquid suction device, the liquid suction device sucks out the liquid in the reaction cup; The mixing device mixes the liquid in the reaction cup after the liquid is injected by the injection device.

2. The automatic magnetic bead cleaning and separation device according to claim 1, characterized in that: The liquid absorbing device comprises a liquid absorbing pad block arranged on the liquid absorbing plate, a liquid absorbing needle penetrating and slidably arranged in the liquid absorbing pad block, and a first buffer structure is arranged on the top of the liquid absorbing needle.

3. The automatic magnetic bead cleaning and separation device according to claim 1, characterized in that: The liquid injection device comprises a bracket arranged on the liquid injection plate, a nut column is arranged on the top of the bracket, a liquid injection needle is penetrated and slidably arranged in the nut column, and a second buffer structure is arranged on the top of the liquid injection needle.

4. The automatic magnetic bead cleaning and separation device according to claim 3 is characterized in that: The mixing device comprises a clamping device for clamping the reaction cup and a power device for driving the clamping device to rotate; the clamping device comprises a rotating cup grabbing shaft rotatably arranged on the liquid suction plate, a rotating synchronous wheel arranged on the rotating cup grabbing shaft and an elastic clamp arranged below the rotating cup grabbing shaft; a top shaft is arranged through the center of the rotating cup grabbing cup, and an injection needle is arranged through the top shaft; upper and lower thrust blocks are arranged at the upper end of the top shaft, and the upper and lower thrust blocks are located below the liquid suction plate; a third buffer structure is arranged between the upper and lower thrust blocks and the rotating synchronous wheel.

5. The automatic magnetic bead cleaning and separation device according to claim 4, characterized in that: The third buffer structure is a thrust spring disposed between the upper and lower thrust blocks and the rotating synchronous wheel.

6. The automatic magnetic bead cleaning and separation device according to claim 4, characterized in that: The liquid injection device is provided with several groups; the power device comprises a synchronous belt which is in contact with the rotating synchronous wheel and a power driving device which drives the synchronous belt to rotate.

7. The automatic magnetic bead cleaning and separation device according to claim 6, characterized in that: The power device also includes a plurality of tension wheels which are in contact with the synchronous belt; among the plurality of tension wheels, at least one sliding tension wheel is provided.

8. The automatic magnetic bead cleaning and separation device according to any one of claims 1 to 7, characterized in that: A driving assembly is provided at the bottom of the disk shaft to drive it to rotate. The driving assembly includes a shaft driven wheel fixedly connected to the disk shaft and a shaft power device to drive the shaft driven wheel to rotate; the output shaft of the shaft power device is provided with a shaft driving wheel, and the shaft driving wheel and the shaft driven wheel are linked by a synchronous belt.

9. The automatic magnetic bead cleaning and separation device according to any one of claims 1 to 7, characterized in that: A code tooth piece is arranged at the bottom of the disk shaft, and a reset photoelectric switch and a rotation photoelectric switch corresponding thereto are arranged at the edge of the code tooth piece; the reset photoelectric switch controls the reset of the disk turntable; and the rotation photoelectric switch controls the rotation amount of the disk turntable.

10. The automatic magnetic bead cleaning and separation device according to any one of claims 1 to 7, characterized in that: A reading device is arranged on the side wall of the disk turntable. The reading device comprises a reading support and a reader arranged in the reading support. The reader reads the light value in the reaction cup.

Images