Biological sample nucleic acid extraction treatment system and treatment method

Abstract

The invention relates to the technical field of biochemical experiment automation equipment, and discloses a biological sample nucleic acid extraction processing system and a processing method.The system comprises a rack and a control unit, a working platform is arranged in the rack, a mechanical arm is arranged above the rack, a module unit is arranged on the working platform, and the control unit is connected with the mechanical arm; comprising a sample tube plate frame, a suction head frame, a suction head recycling box, a pore plate frame, a low-temperature incubation unit, a low-temperature swing unit, a tube plate opening and closing cover unit, a low-speed centrifugal unit, a heating unit, a magnetic frame unit, a PCR unit and a fluorescence detection unit, the sample tube plate frame is arranged in the center of a workbench, and other unit modules are arranged around the sample tube frame; a clamping jaw and a liquid injection unit are arranged on the mechanical arm, and the control unit controls the mechanical arm and each module unit to act, so that a nucleic acid extraction treatment flow is realized. Branch processes are completed through the module units, the control unit connects the processes in series to complete nucleic acid extraction, and multiple processes are achieved in a user-defined mode.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical experiment automation equipment, and in particular to a biological sample nucleic acid extraction and processing system. Background Art

[0002] The nucleic acid extraction experiment of biological samples is a complex process, with long operation and detection time. When there are a large number of samples, more staff members are often required to participate, and it is time-consuming and has low accuracy. Taking the extraction of nucleic acid from animal and plant cells as an example, it often takes more than ten days or more to complete a process.

[0003] With the development of laboratory automation technology, it has become a reality to implement the nucleic acid detection process through an automated platform. Then, this automation process mainly implements multiple related sub-processes separately, and then combines them into a complete process work line. This form can be well implemented for a single experimental process. However, for different samples, the process is slightly different, and the entire laboratory automation equipment must be greatly modified and replaced. For multi-process experiments, it can no longer meet the requirements. Summary of the invention

[0004] The purpose of the present invention is to solve the above problems and provide a biological sample nucleic acid extraction and processing system and method, wherein each module unit completes the branch process, and the control unit connects the process in series to complete the nucleic acid extraction, and realizes customized implementation for multiple processes.

[0005] The technical solution adopted by the present invention is: A biological sample nucleic acid extraction and processing system, characterized in that it includes a frame and a control unit, a working platform is arranged in the frame, a mechanical arm is arranged above the frame, and a module unit is arranged on the working platform, including Sample tube and plate rack, tip rack, tip recovery box, well plate rack, low temperature incubation unit, low temperature swing unit, tube plate opening and closing unit, low speed centrifugation unit, heating unit, magnetic rack unit, PCR unit and fluorescence detection unit, The sample tube rack is arranged at the center of the workbench, and other unit modules are arranged around the sample tube rack. The mechanical arm is provided with a clamping claw and a liquid injection unit. The sample tube plate rack is used to place the sample tube plate, and the tube plate clamp is used to take and place the sample tube plate, and the sample tube is placed on the tube plate; The liquid injection unit completes the liquid processing in the sample tube by loading and unloading the suction tip; The tip recovery box is used to collect unloaded tips and waste liquid; The orifice plate rack is used for dispensing liquid from sample tubes; The tube sheet opening and closing cover unit is used to open and close the cover of the sample tube on the tube sheet; The magnetic frame unit is used to adsorb magnetic beads, and the magnetic beads are bound to nucleic acid substances; The PCR unit and the fluorescence detection unit realize the amplification and quantification of nucleic acid substances. The control unit controls the mechanical arm and each module unit to perform actions to implement the nucleic acid extraction process.

[0006] Furthermore, the module unit also includes an oscillating crushing unit and a high-speed centrifugal unit, which are arranged outside the other module units. The oscillating crushing unit is used to crush the biological sample to obtain nucleic acid substances, and the high-speed centrifugal unit centrifuges the crushed sample.

[0007] Furthermore, a sample tube plate is arranged on the sample tube plate rack, and the sample tube plate is provided with four channels for placing four sample tubes, the sample tubes are centrifuge tubes, and a positioning installation hole is arranged at the end of the sample tube plate for universal installation on each module unit.

[0008] Furthermore, a card block array in the front-to-back direction is arranged on the working platform, card slots are arranged on both sides of the base of each module unit, and each module unit is sequentially mounted on the working platform in multiple rows through the card blocks and the card slots.

[0009] Furthermore, the sample tube plate rack, the tip rack, and the well plate rack are all multiple and arranged in one or more rows. The tip recovery box is arranged outside the tip rack, and other module units are arranged in one or more rows on the periphery.

[0010] Furthermore, multiple rows of sample tube plates are arranged on the sample tube plate rack, a tip array is arranged on the tip rack, the tip array includes tips of various specifications, a well plate is arranged on the well plate rack, the clamp is used for taking and placing the sample tube plates and the well plates, and the injection unit is a four-channel pipette.

[0011] Further, the low temperature incubation unit comprises A low temperature module and a container rack installed on the low temperature module, wherein the container rack corresponds to containers of various specifications; The low-temperature swing unit includes a low-temperature module and a swing module, and the swing module drives the low-temperature module and the sample tube plate on the low-temperature module to swing and mix; The tube sheet opening and closing cover unit comprises a tube sheet seat and an opening and closing pressure plate, and the sample tube on the tube sheet seat is opened and closed by turning the pressure plate; The low-speed centrifugal unit includes a tube sheet seat and a centrifugal motor. The tube sheet seats are symmetrically arranged. The centrifugal motor drives the tube sheet seat to rotate slowly to achieve centrifugation. The heating unit includes a tube sheet seat and an auxiliary heating table. The heating plate arranged on the tube sheet seat heats the sample tube, and the auxiliary heating table presses the heated hot plate onto the heating plate for heat preservation. The magnetic frame unit comprises a magnet seat arranged in a frame, a magnet is arranged on the magnet seat, a tube plate is arranged on the frame, and the sample tubes on the tube plate cooperate with the magnets to achieve adsorption of magnetic beads in the sample tubes on the tube wall.

[0012] Furthermore, the oscillation and crushing unit includes a lifting seat and a driving motor. The driving motor drives the lifting seat to oscillate up and down through a crank wheel. The sample tube in the lifting seat contains biological samples and rigid particles. The high-speed oscillation of the lifting seat causes the rigid particles to crush the biological samples.

[0013] A method for extracting and processing nucleic acid from a biological sample, characterized in that: The process includes: (1) After the biological sample is broken by the oscillating breaking unit and centrifuged by the high-speed centrifugal unit, the supernatant is sucked out by the injection unit and sent to the sample tube on the sample tube plate rack; (2) The clamp removes the sample tube sheet and sends it to the tube sheet opening and closing cover unit. The tube sheet opening and closing cover unit opens the cover of the tube sheet. The liquid injection unit performs one of liquid injection, liquid aspiration, and blowing and aspiration mixing on the sample tube on the sample tube sheet. Then, the tube sheet opening and closing cover unit closes the cover on the sample tube, and the clamp outputs the sample tube sheet. (3) The gripper sends the sample tube plate to the low-temperature incubation unit or the heating unit for cooling or heating. After completion, the gripper outputs the sample tube plate; (4) The injection unit removes the tip from the tip rack, performs liquid operations on the sample tube, and then discards the tip into the tip recovery box; (5) The clamp sends the sample tube plate to the low-temperature swing unit. The low-temperature swing unit swings and oscillates the sample tube according to the set time and temperature. After completion, the clamp outputs the sample tube plate; (6) The clamping jaws send the sample tube plate to the low-speed centrifugal unit, which throws off the vapor and liquid in the sample tube; (7) The injection unit transfers liquid from the sample tube to the well plate of the well plate rack; (8) The clamping claw sends the sample tube plate to the magnetic rack unit, so that the magnetic beads adsorbing nucleic acid in the sample tube adhere to the tube wall or the bottom of the tube, and then the clamping claw outputs the sample tube rack; (9) The gripper takes the sample tube and places it in the PCR unit for amplification, and then sends it to the fluorescence detection unit for quantification. The above processes are independent of each other and are controlled by the control unit.

[0014] Furthermore, multiple processes in the above process are repeated and / or combined to form a complete nucleic acid detection process, and the control unit runs the entire process in the processing system.

[0015] The beneficial effects of the present invention are: (1) It covers all processes in the nucleic acid testing experimental process, including sample processing, liquid processing, nucleic acid extraction, and amplification testing, providing protection for the entire process of nucleic acid testing; (2) After the process is analyzed by the control unit, each process is combined to form a complete detection process; (3) Suitable for various nucleic acid detection processes targeting biological cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Attached Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention; Attached Figure 2 It is a schematic diagram of the arrangement of the module units; Attached Figure 3 This is a partial enlarged view of the working platform’s card-mounted structure; Attached Figure 4 It is a partial enlarged view of the sample tube plate rack arrangement; Attached Figure 5 is a schematic diagram of the structure of the sample tube plate; Attached Figure 6 It is a partial enlarged view of the arrangement of the tip rack and the well plate rack; Attached Figure 7 This is a partial enlarged view of the mechanical part inside the frame; Attached Figure 8 It is a partial enlarged view of the gripper; Attached Fig. 9 It is a partial enlarged view of the tube sheet opening and closing cover unit; Attached Fig.10 It is a partial enlarged view of the magnetic frame unit; Attached Fig.11 It is a partial enlarged view of the oscillating crushing unit; Attached Fig.12 It is a partial enlarged picture of the low-temperature incubation unit; Attached Fig.13 It is a partial enlarged view of the low temperature swing unit; Attached Fig.14 It is a partial enlarged view of the low-speed centrifugal unit; Attached Fig.15 It is a partial enlarged view of the heating unit; Attached Fig.16 It is a partial enlarged view of the PCR unit and the fluorescence detection unit.

[0017] The reference numerals in the accompanying drawings are: 1. Frame; 2. Working platform; 3. Robotic arm; 4. Module unit; 5. Sample tube and plate rack; 6. Tip rack; 7. Tip recovery box; 8. Perforated plate rack; 9. Low temperature incubation unit; 10. Low temperature swing unit; 11.Tube sheet opening and closing cover unit; 12.Low speed centrifugal unit; 13. Heating unit; 14. Magnetic frame unit; 15. PCR unit; 16. Fluorescence detection unit; 17. Card block array; 18. Sample tube plate; 19. Positioning installation hole; 20. Tube sheet seat; 21. Opening and closing pressure plate; 22. Magnet seat; 23. Magnet; 24. Frame; 25. Oscillating crushing unit; 26. High-speed centrifugal unit; 27. Lifting seat; 28. Driving motor; 29. Crank wheel; 30. Low temperature module; 31. Container rack; 32. Low temperature module; 33. Swing module; 34. Tube sheet seat; 35. Tube sheet seat; 36. Auxiliary heating table; 37. Heating plate; 38. X axis; 39.Y axis; 40.Liquid injection unit; 41. Gripper. DETAILED DESCRIPTION

[0018] The specific implementation of the biological sample nucleic acid extraction and processing system and processing method of the present invention is described in detail below with reference to the accompanying drawings.

[0019] See attached Figure 1 The biological sample nucleic acid extraction and processing system of the present invention comprises a rack 1 and a control unit. A working platform 2 is arranged in the rack 1, a mechanical arm 3 is arranged above the rack 1, and a module unit 4 is arranged on the working platform 2. After the control unit sets the process program, it drives the mechanical arm 3 and each module unit 4 to implement the process experiment process. The staff only needs to provide various sample solutions used in the experiment process to the predetermined module unit 4.

[0020] See attached Figure 2 The module unit 4 mainly includes: a sample tube plate rack 5, a tip rack 6, a tip recovery box 7, a well plate rack 8, a low-temperature incubation unit 9, a low-temperature swing unit 10, a tube plate opening and closing cover unit 11, a low-speed centrifugation unit 12, a heating unit 13, a magnetic rack unit 14, a PCR unit 15 and a fluorescence detection unit 16, etc.

[0021] According to the usage of samples and consumables during the experiment, consumables such as sample tubes and pipette tips are pre-arranged on the sample tube plate rack 5 .

[0022] See attached Figure 3 , each module unit 4 in the processing system is installed on the working platform 2, and a card block array 17 in the front-to-back direction is provided on the working platform 2. Card slots are provided on both sides of the base of each module unit 4, and each module unit 4 is card-mounted on the working platform 2 in multiple rows in sequence through the card blocks and the card slots. The card-mounting method can easily change the arrangement of each module unit 4, providing the most economical working line for a specific nucleic acid detection process.

[0023] See attached Figure 4 , 5 The sample tube plate rack 5 is arranged at the central position of the working platform 2, and other unit modules are arranged around the sample tube rack. Multiple rows of sample tube plates 18 are arranged on the sample tube plate rack 5. The sample tube plate 18 is provided with four channels for placing four sample tubes. The sample tubes are centrifuge tubes. A positioning installation hole 19 is provided at the end of the sample tube plate 18, which is universally installed on each module unit 4.

[0024] There are multiple sample tube plate racks 5 on the working platform 2, which can be arranged side by side or in separate places on the platform.

[0025] See attached Figure 6 There are multiple tip racks 6, which are mainly arranged outside the sample tube plate rack 5. A tip recovery box 7 is arranged outside the tip rack 6. Each tip rack 6 is provided with a tip array, which includes tips of various specifications for liquid operations for different sample containers. The tip recovery box is used to recycle used tips and collect waste liquid.

[0026] There are multiple well plate racks 8 distributed at multiple positions outside the sample tube plate rack 5. Well plates are arranged on the well plate racks 8 to meet the liquid separation requirements in the pre-treatment process of nucleic acid detection.

[0027] See attached Figure 7 , 8 The robot arm 3 is provided with a clamp 41 and a liquid injection unit 40. The clamp 41 is used to take and place the sample tube plate 18, sample tubes and well plates. The structure of the clamp 41 has multiple grasping points, which can grasp containers and materials of different sizes respectively.

[0028] The injection unit 40 is a four-channel pipette, which corresponds to the position of the pipette tips in the pipette tip rack 6 and the position of the sample tubes in the sample tube rack 5, and can load four pipette tips at a time and process samples in four sample tubes at the same time, performing pipetting, blowing and aspiration and other actions.

[0029] The robot arm 3 adopts a transverse X-axis 38 located at the front and rear ends of the frame 1, and the Y-axis 39 is straddled on the X-axis 38. The injection unit 40 and the clamp 41 arranged on the Y-axis 39 can cover all module units 4 of the entire working platform 2.

[0030] See attached Fig. 9 The tube sheet opening and closing cover unit 11 is used to open and close the cover of the sample tube on the tube sheet. The tube sheet opening and closing cover unit 11 includes a tube sheet seat 20 and an opening and closing pressure plate 21. The opening and closing cover of the sample tube on the tube sheet seat is realized by the flipping action of the opening and closing pressure plate 21. The flipping action of the opening and closing pressure plate 21 is realized by the motion guide path of the opening and closing pressure plate 21. According to the opening and closing principle of the centrifugal tube, the pressure plate realizes the pressing and closing actions.

[0031] See attached Fig.10 The magnetic frame unit 14 is used to adsorb magnetic beads. The magnetic beads have been combined with nucleic acid substances in the previous operation. The magnetic frame unit 14 includes a magnet seat 22 arranged in the frame, and a magnet 23 is arranged on the magnet seat 22. A sample tube plate 18 is arranged on the frame 24. The sample tubes of the sample tube plate 18 correspond to the magnets 23 to achieve adsorption of the magnetic beads in the sample tubes to the tube walls.

[0032] See attached Fig.11 The module unit 4 is also equipped with an oscillating and crushing unit 25 and a high-speed centrifugal unit 26. In an experiment where no nucleic acid sample is provided, the cells are directly pre-treated. The oscillating and crushing unit 25 is used to crush the biological sample to obtain nucleic acid substances, and the high-speed centrifugal unit 26 centrifuges the crushed sample. The oscillating and crushing unit 25 includes a lifting seat 27 and a driving motor 28. The driving motor 28 drives the lifting seat 27 to oscillate up and down through a crank wheel 29. The sample tube in the lifting seat 27 contains biological samples and rigid particles. The high-speed oscillation of the lifting seat causes the rigid particles to crush the biological sample.

[0033] See attached Fig.12 The low-temperature incubation unit 9 includes a low-temperature module 30 and a container rack 31 installed on the low-temperature module 30. The container rack 31 corresponds to containers of various specifications. The low-temperature incubation unit 9 can be multiple, respectively for containers of different specifications, such as 1ml and 5ml centrifuge tubes.

[0034] See attached Fig.13 The low-temperature swing unit 10 includes a low-temperature module 32 and a swing module 33. The swing module 33 drives the low-temperature module 32 and the sample tube plate 18 on the low-temperature module 32 to swing and mix. The low-temperature swing unit 10 realizes oscillation of the sample in a low-temperature environment.

[0035] See attached Fig.14The low-speed centrifugal unit 12 includes a tube sheet seat 34 and a centrifugal motor. The tube sheet seat 34 is symmetrically arranged. The centrifugal motor drives the tube sheet seat to rotate slowly to achieve centrifugation. The tube sheet seat 34 can also swing freely to achieve a tube throwing similar to manual operation, so that the gas and liquid on the tube wall fall back.

[0036] See attached Fig.15 The heating unit 13 includes a tube sheet seat 35 and an auxiliary heating table 36. The heating plate arranged on the tube sheet seat 35 heats the sample tube, and the auxiliary heating table 36 presses the heated hot plate 37 onto the heating plate for heat preservation.

[0037] See attached Fig.16 , the PCR unit 15 and the fluorescence detection unit 16 realize the amplification and quantification of the nucleic acid substance. Both of them are products in the existing technology. The equipment is controlled by the control unit and the measurement data is fed back to the system.

[0038] This processing system can complete the following processes: (1) After the biological sample is broken by the oscillating breaking unit 25 and centrifuged by the high-speed centrifugal unit 26, the supernatant is sucked by the injection unit and sent to the sample tube on the sample tube plate rack 5; (2) The clamp removes the sample tube sheet 18 and sends it to the tube sheet cover opening and closing unit 11. The tube sheet cover opening and closing unit 11 opens the tube sheet, and the liquid injection unit performs one of the following steps: liquid injection, liquid aspiration, and mixing by blowing and suction on the sample tube on the sample tube sheet 18. Then, the tube sheet cover opening and closing unit 11 closes the cover on the sample tube, and the clamp outputs the sample tube sheet 18. (3) The clamping jaws deliver the sample tube plate 18 to the low-temperature incubation unit 9 and the heating unit 13 for cooling or heating operations, and after completion, the clamping jaws output the sample tube plate 18; (4) The liquid injection unit removes the pipette tip from the pipette tip rack 6, performs liquid operation on the sample tube, and then discards the pipette tip into the pipette tip recovery box 7; (5) The clamping jaws deliver the sample tube plate 18 to the low-temperature swing unit 10. The low-temperature swing unit 10 swings and oscillates the sample tube according to the set time and temperature. After completion, the clamping jaws output the sample tube plate 18. (6) The clamping jaws deliver the sample tube plate 18 to the low-speed centrifugal unit 12, and the low-speed centrifugal unit 12 throws off the vapor and liquid in the sample tube; (7) The injection unit transfers liquid from the sample tube to the well plate of the well plate rack 8; (8) The clamp delivers the sample tube plate 18 to the magnetic rack unit 14, so that the magnetic beads adsorbing nucleic acid in the sample tube adhere to the tube wall or the bottom of the tube, and then the clamp outputs the sample tube rack; (9) The gripper takes the sample tube and places it in the PCR unit for amplification, and then sends it to the fluorescence detection unit 16 for quantification.

[0039] It also includes the processing of the time axis, including static, heat preservation and cold preservation, etc. Each process can be used as a branch process of the control unit. By combining the above multiple processes or one process and repeated operations with other processes, a complex process can be completed to achieve sample nucleic acid detection and processing. As long as the branch processes after the complex process is decomposed can be realized in the above equipment, the new process can be completed through the configuration of the control unit.

[0040] The above are only preferred embodiments of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and modifications without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A biological sample nucleic acid extraction and processing system, characterized in that: It includes a frame and a control unit, a working platform is arranged in the frame, a mechanical arm is arranged above the frame, and a module unit is arranged on the working platform, including Sample tube and plate rack, tip rack, tip recovery box, well plate rack, low temperature incubation unit, low temperature swing unit, tube plate opening and closing unit, low speed centrifugation unit, heating unit, magnetic rack unit, PCR unit and fluorescence detection unit, The sample tube rack is arranged at the center of the workbench, and other unit modules are arranged around the sample tube rack. The mechanical arm is provided with a clamping claw and a liquid injection unit. The sample tube plate rack is used to place the sample tube plate, and the tube plate clamp is used to take and place the sample tube plate, and the sample tube is placed on the tube plate; The liquid injection unit completes the liquid processing in the sample tube by loading and unloading the suction tip; The tip recovery box is used to collect unloaded tips and waste liquid; The orifice plate rack is used for dispensing liquid from sample tubes; The tube sheet opening and closing cover unit is used to open and close the cover of the sample tube on the tube sheet; The magnetic frame unit is used to adsorb magnetic beads, and the magnetic beads are bound to nucleic acid substances; The PCR unit and the fluorescence detection unit realize the amplification and quantification of nucleic acid substances. The control unit controls the mechanical arm and each module unit to perform actions to implement the nucleic acid extraction process.

2. The biological sample nucleic acid extraction and processing system according to claim 1, characterized in that: The module unit also includes an oscillating crushing unit and a high-speed centrifugal unit, which are arranged outside the other module units. The oscillating crushing unit is used to crush the biological sample to obtain nucleic acid substances, and the high-speed centrifugal unit centrifuges the crushed sample.

3. The biological sample nucleic acid extraction and processing system according to claim 1, characterized in that: The sample tube plate rack is provided with a sample tube plate, which is provided with four channels for placing four sample tubes, the sample tubes being centrifuge tubes, and a positioning installation hole is provided at the end of the sample tube plate for universal installation on each module unit.

4. The biological sample nucleic acid extraction and processing system according to any one of claims 1 to 3, characterized in that: The work platform is provided with a card block array in the front-to-back direction, and card slots are provided on both sides of the base of each module unit. Each module unit is sequentially mounted on the work platform in multiple rows through the card blocks and the card slots.

5. The biological sample nucleic acid extraction and processing system according to claim 4, characterized in that: The sample tube plate rack, the tip rack, and the well plate rack are all multiple and arranged in one or more rows. The tip recovery box is arranged outside the tip rack, and other module units are arranged in one or more rows on the periphery.

6. The biological sample nucleic acid extraction and processing system according to any one of claims 1 to 3, characterized in that: The sample tube plate rack is provided with multiple rows of sample tube plates, the tip rack is provided with a tip array, the tip array includes tips of various specifications, the well plate rack is provided with a well plate, the clamp is used for taking and placing the sample tube plates and the well plate, and the injection unit is a four-channel pipette.

7. The biological sample nucleic acid extraction and processing system according to any one of claims 1 to 3, characterized in that: The low-temperature incubation unit comprises A low temperature module and a container rack installed on the low temperature module, wherein the container rack corresponds to containers of various specifications; The low-temperature swing unit includes a low-temperature module and a swing module, and the swing module drives the low-temperature module and the sample tube plate on the low-temperature module to swing and mix; The tube sheet opening and closing cover unit comprises a tube sheet seat and an opening and closing pressure plate, and the sample tube on the tube sheet seat is opened and closed by turning the pressure plate; The low-speed centrifugal unit includes a tube sheet seat and a centrifugal motor. The tube sheet seats are symmetrically arranged. The centrifugal motor drives the tube sheet seat to rotate slowly to achieve centrifugation. The heating unit includes a tube sheet seat and an auxiliary heating table. The heating plate arranged on the tube sheet seat heats the sample tube, and the auxiliary heating table presses the heated hot plate onto the heating plate for heat preservation. The magnetic frame unit comprises a magnet seat arranged in a frame, a magnet is arranged on the magnet seat, a tube plate is arranged on the frame, and the sample tubes on the tube plate cooperate with the magnets to achieve adsorption of magnetic beads in the sample tubes on the tube wall.

8. The biological sample nucleic acid extraction and processing system according to claim 2, characterized in that: The oscillation and crushing unit comprises a lifting seat and a driving motor. The driving motor drives the lifting seat to oscillate up and down through a crank wheel. The sample tube in the lifting seat contains biological samples and rigid particles. The high-speed oscillation of the lifting seat causes the rigid particles to crush the biological samples.

9. A method for extracting and processing nucleic acid from a biological sample, using the biological sample nucleic acid extraction and processing system as claimed in any one of claims 1 to 7, characterized in that: The process includes: (1) After the biological sample is broken by the oscillating breaking unit and centrifuged by the high-speed centrifugal unit, the supernatant is sucked out by the injection unit and sent to the sample tube on the sample tube plate rack; (2) The clamp removes the sample tube sheet and sends it to the tube sheet opening and closing cover unit. The tube sheet opening and closing cover unit opens the cover of the tube sheet. The liquid injection unit performs one of liquid injection, liquid aspiration, and blowing and aspiration mixing on the sample tube on the sample tube sheet. Then, the tube sheet opening and closing cover unit closes the cover on the sample tube, and the clamp outputs the sample tube sheet. (3) The gripper sends the sample tube plate to the low-temperature incubation unit or the heating unit for cooling or heating. After completion, the gripper outputs the sample tube plate; (4) The injection unit removes the tip from the tip rack, performs liquid operations on the sample tube, and then discards the tip into the tip recovery box; (5) The clamp sends the sample tube plate to the low-temperature swing unit. The low-temperature swing unit swings and oscillates the sample tube according to the set time and temperature. After completion, the clamp outputs the sample tube plate; (6) The clamping jaws send the sample tube plate to the low-speed centrifugal unit, which throws off the vapor and liquid in the sample tube; (7) The injection unit transfers liquid from the sample tube to the well plate of the well plate rack; (8) The clamping claw sends the sample tube plate to the magnetic rack unit, so that the magnetic beads adsorbing nucleic acid in the sample tube adhere to the tube wall or the bottom of the tube, and then the clamping claw outputs the sample tube rack; (9) The gripper takes the sample tube and places it in the PCR unit for amplification, and then sends it to the fluorescence detection unit for quantification. The above processes are independent of each other and are controlled by the control unit.

10. The method for extracting and processing nucleic acid from a biological sample according to claim 9, characterized in that: Multiple processes in the above process are repeated and / or combined to form a complete nucleic acid detection process, and the control unit runs the entire process in the processing system.

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