Non-contact biological particle treatment equipment

Abstract

The invention discloses non-contact biological particle treatment equipment which comprises a containing device and a triggering device. The containing device comprises a light sensing structure, a matching structure spaced from the light sensing structure and a frame arranged between the light sensing structure and the matching structure. The frame is provided with two working sections which are opposite to each other, and a first opening and a second opening smaller than the first opening are formed in the two opposite ends of the frame respectively. And the trigger device is arranged corresponding to the frame. When at least one biological particle with the size larger than that of the second opening penetrates through the first opening and enters the space between the two working sections, the triggering device can be used for triggering the cell outer surface layer of at least one biological particle so that the cell outer surface layer can have the preset permeability larger than the initial permeability. Therefore, the permeability of the cell outer surface layer of the target biological particle can be improved according to requirements, and various treatment operations can be accurately carried out.

Description

Technical Field

[0001] The present invention relates to a biological particle processing device, in particular to a non-contact biological particle processing device. Background Art

[0002] Existing biological particle processing devices can drive biological particles to move by applying light driving. However, it is difficult for existing biological particle processing devices to accurately control the biological particles to perform related processing operations at a fixed point. Therefore, the inventors believe that the above defects can be improved, and have devoted themselves to research and applied scientific principles, and finally proposed a reasonable design and effective improvement of the above defects. Summary of the invention

[0003] The embodiment of the present invention provides a non-contact biological particle processing device, which can effectively improve the defects that may occur in existing biological particle processing devices.

[0004] The embodiment of the present invention discloses a non-contact biological particle processing device, which includes: a containing device, which is used to contain a liquid specimen, which contains a plurality of biological particles and a transfection substance, and the containing device includes: a light-sensing structure, which has a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure, which is separated from the light-sensing structure; wherein at least one of the light-sensing structure and the matching structure is transparent, and the matching structure includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light-sensing structure; and a frame, which is arranged between the light-sensing structure and the matching structure; wherein the frame has two working sections facing each other, and the opposite ends of the frame are respectively formed with a first opening and a second opening smaller than the first opening; wherein at least one of the plurality of biological particles is defined as a mesh The target biological particle has a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; a light capturing device facing the containing device; wherein the light capturing device can be used to form a first dielectrophoretic pattern on the light sensing structure, so as to drive the target biological particle through the first opening and enter between the two working sections through the first dielectrophoretic pattern; and a trigger device, which corresponds to the frame setting; wherein the trigger device can be used to trigger the cell surface layer of the target biological particle located between the two working sections, so that the cell surface layer has a predetermined permeability greater than the initial permeability; wherein, when the target biological particle is captured between the two working sections, the light capturing device can be used to form a second dielectrophoretic pattern on the light sensing structure, so as to drive the transfection material through the second opening and enter between the two working sections through the second dielectrophoretic pattern, so as to realize a transfection operation.

[0005] Optionally, the area between the two working sections is formed with: a receiving area, one end of which is defined by a first opening; and a necking area, which is located at the other end of the receiving area; wherein the necking area is gradually tapered in a direction away from the receiving area, and the necking area is defined by a second opening away from the first opening.

[0006] Optionally, the trigger device includes: two electrode pads, located in the receiving area and respectively arranged on the inner walls of the two working sections; and a power source, electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the receiving area, the power source can drive the two electrode pads to apply an electric field to the target biological particles, so that the target biological particles form an electroporation, thereby making the cell surface layer have a predetermined permeability.

[0007] Optionally, the trigger device includes: two electrode pads located in the necking area and respectively arranged on the inner walls of the two working sections; and a power source electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the necking area, the power source can drive the two electrode pads to apply an electric field to the target biological particles, so that the target biological particles form an electroporation, thereby making the cell surface layer have a predetermined permeability.

[0008] Optionally, the frame includes two channel sections, and the two channel sections are respectively connected to two working section parts defining the second opening; wherein the two channel sections define a third opening away from the second opening, which is larger than the second opening.

[0009] Optionally, when the target biological particles are captured between the two working sections, the light capturing device can be used to form a third dielectrophoretic pattern on the light sensing structure, so as to maintain the target biological particles between the two working sections through the third dielectrophoretic pattern.

[0010] Optionally, the transfection material comprises at least one of RNA, DNA, exosomes, liposomes and viruses.

[0011] Optionally, the transfection material includes at least one of RNA, DNA, exosomes and liposomes, and during the implementation of the transfection operation, the target biological particles are captured between the two working sections and have a predetermined permeability.

[0012] Optionally, the transfection material includes a virus, and during the transfection operation, the target biological particles are captured between the two working sections and have an initial permeability.

[0013] The embodiment of the present invention also discloses a non-contact biological particle processing device, which includes: a containing device, which is used to contain a liquid specimen, which contains a plurality of biological particles, and the containing device includes: a light sensing structure, which has a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure, which is separated from the light sensing structure; wherein at least one of the light sensing structure and the matching structure is transparent, and the matching structure includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light sensing structure; and a frame, which is arranged between the light sensing structure and the matching structure; wherein the frame has two working sections facing each other, and the opposite ends of the frame respectively form a first opening and a second opening smaller than the first opening; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and The target biological particle has an initial permeability on its cell surface; a light capturing device facing the containing device; wherein the light capturing device can be used to form a first dielectrophoretic pattern on the light sensing structure, so as to drive the target biological particle through the first opening and enter between the two working sections through the first dielectrophoretic pattern; and a triggering device, which corresponds to the frame arrangement; wherein the triggering device can be used to trigger the cell surface of the target biological particle located between the two working sections, so as to make the cell surface have a predetermined permeability greater than the initial permeability, thereby causing the target biological particle to proliferate an exosome that passes through the cell surface; wherein, when the target biological particle is captured between the two working sections and proliferates with exosomes, the light capturing device can be used to form a second dielectrophoretic pattern on the light sensing structure, so as to drive the exosome from between the two working sections through the second opening and leave the two working sections through the second dielectrophoretic pattern, so as to achieve a purification operation.

[0014] Optionally, the area between the two working sections is formed with: a receiving area, one end of which is defined by a first opening; and a necking area, which is located at the other end of the receiving area; wherein the necking area is gradually tapered in a direction away from the receiving area, and the necking area is defined by a second opening away from the first opening.

[0015] Optionally, the trigger device includes: two electrode pads, located in the receiving area and respectively arranged on the inner walls of the two working sections; and a power source, electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the receiving area, the power source can drive the two electrode pads to apply an electric field to the target biological particles, so that the target biological particles form an electroporation, thereby making the cell surface layer have a predetermined permeability.

[0016] Optionally, the trigger device includes: two electrode pads located in the necking area and respectively arranged on the inner walls of the two working sections; and a power source electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the necking area, the power source can drive the two electrode pads to apply an electric field to the target biological particles, so that the target biological particles form an electroporation, thereby making the cell surface layer have a predetermined permeability.

[0017] Optionally, the frame includes two channel sections, and the two channel sections are respectively connected to two working section parts defining the second opening; wherein the two channel sections define a third opening away from the second opening, which is larger than the second opening.

[0018] The embodiment of the present invention further discloses a non-contact biological particle processing device, which includes: a containing device, which is used to contain a liquid specimen, which contains a plurality of biological particles, and the containing device includes: a light sensing structure, which has a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure, which is separated from the light sensing structure; wherein at least one of the light sensing structure and the matching structure is transparent, and the matching structure includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light sensing structure; and a frame, which is arranged between the light sensing structure and the matching structure. The invention relates to a method for preparing a novel bioparticle-enhanced bioparticle system comprising: a frame having two working sections facing each other, and two opposite ends of the frame having a first opening and a second opening smaller than the first opening, respectively; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and a cell surface layer of the target biological particle has an initial permeability; and a trigger device, which is arranged corresponding to the frame; wherein when the target biological particle passes through the first opening and enters between the two working sections, the trigger device can be used to trigger the cell surface layer of the target biological particle so that the cell surface layer has a predetermined permeability greater than the initial permeability.

[0019] In summary, the non-contact biological particle processing equipment disclosed in the embodiment of the present invention cooperates with the frame and the trigger device and is matched with the light sensing structure of the containing device, so that the frame can be used to locate the target biological particles, and the trigger device can increase the permeability of the cell surface layer of the target biological particles according to needs, thereby facilitating the precise processing of the target biological particles (such as the transfection operation or the purification operation).

[0020] To further understand the features and technical contents of the present invention, please refer to the following detailed description and drawings of the present invention. However, such description and drawings are only used to illustrate the present invention and are not intended to limit the protection scope of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a three-dimensional schematic diagram of the non-contact biological particle processing equipment according to the first embodiment of the present invention.

[0022] Figure 2 It is a schematic plan cross-sectional view of a non-contact biological particle processing device according to the first embodiment of the present invention.

[0023] Figure 3 It is a three-dimensional cross-sectional schematic diagram of a non-contact biological particle processing device according to a first embodiment of the present invention.

[0024] Figure 4 for Figure 2 A cross-sectional schematic diagram of another embodiment.

[0025] Figure 5 for Figure 2 A cross-sectional schematic diagram of the subsequent action of .

[0026] Figure 6 for Figure 5 A cross-sectional schematic diagram of the subsequent action of .

[0027] Figure 7 It is a cross-sectional schematic diagram of a non-contact biological particle processing device according to a second embodiment of the present invention.

[0028] Figure 8 It is a cross-sectional schematic diagram of a non-contact biological particle processing device according to a third embodiment of the present invention.

[0029] Fig. 9 for Figure 8 A cross-sectional schematic diagram of the subsequent action of .

[0030] Fig.10 for Fig. 9 A cross-sectional schematic diagram of the subsequent action of . DETAILED DESCRIPTION

[0031] The following is an explanation of the implementation of the "non-contact biological particle processing equipment" disclosed in the present invention through specific embodiments. Those skilled in the art can understand the advantages and effects of the present invention from the contents disclosed in this specification. The present invention can be implemented or applied through other different specific embodiments, and the details in this specification can also be modified and changed based on different viewpoints and applications without departing from the concept of the present invention. In addition, the drawings of the present invention are only simple schematic illustrations and are not depicted according to actual dimensions. It is stated in advance. The following implementation methods will further explain the relevant technical contents of the present invention in detail, but the disclosed contents are not intended to limit the scope of protection of the present invention.

[0032] It should be understood that, although the terms "first", "second", "third", etc. may be used herein to describe various components or signals, these components or signals should not be limited by these terms. These terms are mainly used to distinguish one component from another component, or one signal from another signal. In addition, the term "or" used herein may include any one or more combinations of the associated listed items depending on the actual situation.

[0033] [Example 1]

[0034] See also Figures 1 to 6 As shown, it is the first embodiment of the present invention. Figures 1 to 3 As shown, this embodiment discloses a non-contact biological particle processing device 100, which includes a containing device 1, an alternating current device 2 electrically coupled to the containing device 1, a light capturing device 3 facing the containing device 1, and a trigger device 4 disposed on the containing device 1, but the present invention is not limited thereto. For example, in other embodiments not shown in the present invention, the non-contact biological particle processing device 100 can omit at least one of the alternating current device 2 and the light capturing device 3 according to actual needs; for example, the combination of the containing device 1 and the trigger device 4 can be used independently (e.g., sold) or used in combination with other devices.

[0035] The container 1 in this embodiment is a rectangular structure of chip-scale, and is used to contain a liquid specimen S, which includes a plurality of biological particles P and a transfection substance T, but the present invention is not limited thereto. For example, the number of biological particles P included in the liquid specimen S can also be adjusted according to actual needs (e.g., at least one).

[0036] It should be additionally explained that the liquid sample S can be a body fluid sample from an animal (such as blood, lymph, saliva or urine), and the biological particles P can be a specific type of cell or cell cluster, such as circulating tumor cells (CTC), fetal nucleated red blood cells (FNRBCs) or bacteria, and the transfection material T has genetic material and can be at least one of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), exosome, liposome and virus, but the present invention is not limited to the above. For example, in other embodiments not shown in the present invention, the liquid sample S can also be a liquid sample from a plant.

[0037] The containing device 1 includes a light sensing structure 11, a matching structure 12 spaced apart from the light sensing structure 11, a plurality of frames 13 disposed between the light sensing structure 11 and the matching structure 12, and a bonding layer 14 connecting the light sensing structure 11 and the matching structure 12. Among them, at least one of the light sensing structure 11 and the matching structure 12 is transparent, and the light sensing structure 11 and the matching structure 12 are two plate-like structures disposed parallel to each other in this embodiment, and the distance between them is greater than the size of any of the biological particles P, but the present invention is not limited to the above.

[0038] In more detail, the light sensing structure 11 has a first substrate 111, a first electrode layer 112 formed on the first substrate 111, and a photoelectric layer 113 formed on the first substrate 111. In this embodiment, the first electrode layer 112 is formed on the bottom side of the first substrate 111, the photoelectric layer 113 is formed on the top side of the first substrate 111, and the photoelectric layer 113 is formed with a plurality of transistors 1131 arranged in a matrix, but the present invention is not limited thereto.

[0039] The matching structure 12 includes a second substrate 121 and a second electrode layer 122 formed on the second substrate 121, and the second electrode layer 122 faces the light sensing structure 11 (e.g., the photoelectric layer 113). In this embodiment, the AC device 2 is electrically coupled to the first electrode layer 112 of the light sensing structure 11 and the second electrode layer 122 of the matching structure 12, so that the light sensing structure 11 can be irradiated by the light emitted by the light capturing device 3 to form a dielectrophoresis pattern F, so as to move at least one of the biological particles P or the transfection substance T in the liquid sample S through the dielectrophoresis pattern F.

[0040] For example, the light capturing device 3 may include a camera 31 and a light source 32 matched with the camera 31. The light capturing device 3 can emit light from the light source 32 to illuminate the light sensing structure 11, so that the light sensing structure 11 forms the dielectrophoresis pattern F.

[0041] The plurality of frames 13 are sandwiched between the photoelectric layer 113 of the light sensing structure 11 and the second electrode layer 122 of the matching structure 12, and since the structures of the plurality of frames 13 are substantially the same in this embodiment, for the sake of convenience, the structure of a single frame 13 will be described below, but the present invention is not limited thereto. For example, in other embodiments not shown in the present invention, the structures of the plurality of frames 13 may also be slightly different.

[0042] In this embodiment, the frame 13 is a mirror-symmetrical structure, and the frame 13 includes two working sections 131 and two channel sections 132 extending from the two working sections 131. The two working sections 131 are spaced apart from each other and arranged facing each other, and the two channel sections 132 are also spaced apart from each other and arranged facing each other, but the present invention is not limited thereto. Figure 4 As shown, the frame 13 may also omit the two channel sections 132 according to actual needs.

[0043] In more detail, the two opposite ends of the two working sections 131 are respectively formed with a first opening 1311 and a second opening 1312 smaller than the first opening 1311. That is to say, the area between the two working sections 131 can be connected to the outside through the first opening 1311 and the second opening 1312 respectively. Furthermore, the two channel sections 132 are respectively connected to the two working sections 131 parts where the second opening 1312 is defined, and the two channel sections 132 are defined with a third opening 1321 away from the second opening 1312, which is larger than the second opening 1312. In this embodiment, the size of the third opening 1321 is substantially equal to the size of the second opening 1312, but the present invention is not limited thereto.

[0044] Furthermore, in the present embodiment, the region between the two working sections 131 is formed with a receiving region 1313 and a necking region 1314 connected to the receiving region 1313, and one end of the receiving region 1313 is defined with the first opening 1311, and the necking region 1314 is defined with the second opening 1312 away from the first opening 1311. The receiving region 1313 has a width substantially the same as that of the first opening 1311, the necking region 1314 is located at the other end of the receiving region 1313, and the necking region 1314 is gradually narrowed in a direction away from the receiving region 1313, thereby defining the second opening 1312, but the present invention is not limited thereto.

[0045] It should be noted that at least one of the plurality of biological particles P is defined as a target biological particle P1, which has a particle size larger than the second opening 1312 but smaller than the first opening 1311, and the cell surface layer P1-1 of the target biological particle P1 has an initial permeability; for example, the cell surface layer P1-1 can be a cell membrane or a cell wall. Furthermore, the size of the transfection substance T is smaller than the second opening 1312.

[0046] like Figure 3 , Figure 5 and Figure 6 As shown, the light capturing device 3 can be used to form a first dielectrophoretic pattern F1 on the light sensing structure 11, so as to drive the target biological particle P1 through the first opening 1311 and enter between the two working sections 131 through the first dielectrophoretic pattern F1. In this embodiment, the light capturing device 3 emits light to irradiate the light sensing structure 11 to form the first dielectrophoretic pattern F1 that is closed and surrounds the target biological particle P1, and then the target biological particle P1 is moved through the first opening 1311 and enters the receiving area 1313 through the first dielectrophoretic pattern F1.

[0047] Furthermore, when the target biological particle P1 is captured within the two working sections 131, the light capturing device 3 can be used to form a second dielectrophoretic pattern F2 on the light sensing structure 11, so as to drive the transfection material T through the second opening 1312 and enter between the two working sections 131 through the second dielectrophoretic pattern F2 to achieve a transfection operation. Accordingly, in the case where the transfection material T includes the virus, during the implementation of the transfection operation, the target biological particle P1 is captured between the two working sections 131 and has the initial permeability.

[0048] It should be noted that when the target biological particle P1 is captured between the two working sections 131, the light capturing device 3 can be used to form a third dielectrophoretic pattern F3 on the light sensing structure 11, so as to maintain the target biological particle P1 between the two working sections 131 through the third dielectrophoretic pattern F3, thereby facilitating the transfection operation. Furthermore, the specific appearance of the second dielectrophoretic pattern F2 and the third dielectrophoretic pattern F3 can be adjusted according to actual needs.

[0049] In addition, although the position of the target biological particle P1 is limited by the third dielectrophoretic pattern F3 in this embodiment, in other embodiments not shown in the present invention, the target biological particle P1 can also be controlled by the pressure of the liquid specimen S, thereby limiting the target biological particle P1 to a specific position.

[0050] In addition, the trigger device 4 is arranged corresponding to the frame 13, and the trigger device 4 can be used to trigger the cell outer layer P1-1 of the target biological particle P1 located between the two working sections 131, so that the cell outer layer P1-1 has a predetermined permeability greater than the initial permeability. It should be noted that the trigger device 4 can use electrical energy or light energy to instantaneously increase the permeability of the cell outer layer P1-1; for example: electroporation, laser transfection, and light injection. However, for ease of understanding, the trigger device 4 is described in this embodiment using the electroporation method, but the present invention is not limited thereto.

[0051] Specifically, the trigger device 4 includes two electrode pads 41 and a power source 42 electrically coupled to the two electrode pads 41. In this embodiment, the power source 42 is described as a DC power source, and the two electrode pads 41 are respectively a positive electrode and a negative electrode (e.g., the two electrode pads 41 are located in the receiving area 1313 and are respectively disposed on the inner walls of the two working sections 131), but the present invention is not limited thereto. For example, in other embodiments not shown in the present invention, the power source 42 may also be an AC power source according to actual needs.

[0052] When the target biological particle P1 is captured in the containing area 1313, the power source 42 can drive the two electrode pads 41 to apply an electric field (e.g., a short-term high-intensity electric field) to at least one of the target biological particles P1, so that the target biological particle P1 forms an electroporation, thereby making the cell surface layer P1-1 have the predetermined permeability.

[0053] Accordingly, when the transfection substance T includes at least one of the RNA, the DNA, the exosome and the liposome, during the implementation of the transfection operation, the target biological particle P1 is captured between the two working sections 131 and has the predetermined permeability through the trigger device 4.

[0054] As described above, the non-contact biological particle processing equipment 100 in this embodiment cooperates with the frame 13 and the trigger device 4 and is matched with the light sensing structure 11 of the containing device 1, so that the frame 13 can be used to locate the target biological particle P1, and the trigger device 4 can improve the permeability of the cell surface layer P1-1 of the target biological particle according to needs, thereby facilitating the precise operation of the target biological particle P1.

[0055] [Example 2]

[0056] See also Figure 7 As shown, it is the second embodiment of the present invention. Since this embodiment is similar to the first embodiment, the similarities between the two embodiments are not repeated here, and the difference between this embodiment and the first embodiment mainly lies in the trigger device 4.

[0057] In this embodiment, the two electrode pads 41 are located in the necking region 1314 and are respectively disposed on the inner walls of the two working sections 131. When the target biological particle P1 is captured in the necking region 1314, the power source 42 can drive the two electrode pads 41 to apply an electric field (e.g., a short-term high-intensity electric field) to the target biological particle P1, so that the target biological particle P1 forms an electroporation, thereby making the cell outer layer P1-1 have the predetermined permeability.

[0058] Accordingly, the light capturing device 3 can confine the target biological particle P1 to the necking region 1314 by the third dielectrophoretic pattern F3, and the cell outer surface layer P1-1 of the target biological particle P1 adjacent to the second opening 1312 can be instantaneously opened by the electroporation, so that the light capturing device 3 can move the transfection substance T through the second opening 1312 to the necking region 1314 by the second dielectrophoretic pattern F2, thereby facilitating a more accurate transfection operation between the target biological particle P1 and the transfection substance T.

[0059] [Example 3]

[0060] See also Figures 8 to 10 As shown, this is the third embodiment of the present invention. Since this embodiment is similar to the above-mentioned embodiments 1 and 2, the same points of the above-mentioned embodiments are not repeated here, and the difference between this embodiment and the above-mentioned embodiments 1 and 2 mainly lies in the operation of the trigger device 4.

[0061] In this embodiment, the trigger device 4 can be used to trigger the cell surface layer P1-1 of the target biological particle P1 located between the two working sections 131, so that the cell surface layer P1-1 has a predetermined permeability greater than the initial permeability, thereby causing the target biological particle P1 to proliferate an exosome T1 (exosome) that passes through the cell surface layer P1-1.

[0062] When the target biological particle P1 is captured between the two working sections 131 and the exosomes T1 proliferate, the light capturing device 3 can be used to form a second dielectrophoretic pattern F2 on the light sensing structure 11, so as to drive the exosomes T1 to pass through the second opening 1312 from between the two working sections 131 and leave the two working sections 131 through the second dielectrophoretic pattern F2, so as to achieve a purification operation.

[0063] [Technical Effects of Embodiments of the Invention]

[0064] In summary, the non-contact biological particle processing equipment disclosed in the embodiment of the present invention cooperates with the frame and the trigger device and is matched with the light sensing structure of the containing device, so that the frame can be used to locate the target biological particles, and the trigger device can increase the permeability of the cell surface layer of the target biological particles according to needs, thereby facilitating the precise processing of the target biological particles (such as the transfection operation or the purification operation).

[0065] The contents disclosed above are only preferred feasible embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Therefore, all equivalent technical changes made using the contents of the present invention's specification and drawings are included in the patent scope of the present invention.

Claims

1. A non-contact biological particle processing device, characterized in that: The non-contact biological particle processing equipment comprises: A container for containing a liquid sample, which contains a plurality of biological particles and a transfection substance, and the container comprises: A light sensing structure comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure, spaced apart from the light sensing structure; wherein at least one of the light sensing structure and the matching structure is transparent, and the matching structure comprises a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light sensing structure; and a frame disposed between the light sensing structure and the matching structure; wherein the frame has two working sections facing each other, and the opposite ends of the frame are respectively formed with a first opening and a second opening smaller than the first opening; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; a light capturing device facing the containing device; wherein the light capturing device can be used to form a first dielectrophoretic pattern on the light sensing structure, so as to drive the target biological particles through the first opening and enter between the two working sections through the first dielectrophoretic pattern; and a trigger device, which is arranged corresponding to the frame; wherein the trigger device can be used to trigger the cell outer layer of the target biological particle located between the two working sections, so that the cell outer layer has a predetermined permeability greater than the initial permeability; When the target biological particles are captured between the two working sections, the light capturing device can be used to form a second dielectrophoretic pattern on the light sensing structure, so as to drive the transfection material through the second opening and enter between the two working sections through the second dielectrophoretic pattern to achieve a transfection operation.

2. The non-contact biological particle processing equipment according to claim 1, characterized in that: The area between the two working sections is formed by: a receiving area, one end of which is defined with the first opening; and A necking region is located at the other end of the receiving region; wherein the necking region is gradually tapered in a direction away from the receiving region, and the necking region defines the second opening away from the first opening.

3. The non-contact biological particle processing equipment according to claim 2, characterized in that: The trigger device comprises: Two electrode pads, located in the receiving area and respectively arranged on the inner walls of the two working sections; and A power source is electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the containment area, the power source can drive the two electrode pads to apply an electric field to the target biological particles so that the target biological particles form an electroporation, thereby making the cell surface layer have the predetermined permeability.

4. The non-contact biological particle processing equipment according to claim 2, characterized in that: The trigger device comprises: Two electrode pads, located in the necking area and respectively arranged on the inner walls of the two working sections; and A power source is electrically coupled to the two electrode pads; wherein, when the target biological particle is captured in the necking area, the power source can drive the two electrode pads to apply an electric field to the target biological particle so that the target biological particle forms an electroporation, thereby making the cell surface layer have the predetermined permeability.

5. The non-contact biological particle processing equipment according to claim 1, characterized in that: The frame includes two channel sections, and the two channel sections are respectively connected to the two working section parts defining the second opening; wherein the two channel sections define a third opening away from the second opening, which is larger than the second opening.

6. The non-contact biological particle processing equipment according to claim 1, characterized in that: When the target biological particles are captured between the two working sections, the light capturing device can be used to form a third dielectrophoretic pattern on the light sensing structure, so as to maintain the target biological particles between the two working sections through the third dielectrophoretic pattern.

7. The non-contact biological particle processing equipment according to claim 1, characterized in that: The transfection material includes at least one of RNA, DNA, exosomes, liposomes and viruses.

8. The non-contact biological particle processing equipment according to claim 1, characterized in that: The transfection material includes at least one of RNA, DNA, exosomes and liposomes, and during the implementation of the transfection operation, the target biological particles are captured between the two working sections and have the predetermined permeability.

9. The non-contact biological particle processing device according to claim 1, characterized in that: The transfection material contains viruses, and during the implementation of the transfection operation, the target biological particles are captured between the two working sections and have the initial permeability.

10. A non-contact biological particle processing device, characterized in that: The non-contact biological particle processing equipment comprises: A containing device, which is used to contain a liquid specimen, which contains a plurality of biological particles, and the containing device comprises: A light sensing structure comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure, spaced apart from the light sensing structure; wherein at least one of the light sensing structure and the matching structure is transparent, and the matching structure comprises a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light sensing structure; and a frame disposed between the light sensing structure and the matching structure; wherein the frame has two working sections facing each other, and the opposite ends of the frame are respectively formed with a first opening and a second opening smaller than the first opening; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; a light capturing device facing the containing device; wherein the light capturing device can be used to form a first dielectrophoretic pattern on the light sensing structure, so as to drive the target biological particles through the first opening and enter between the two working sections through the first dielectrophoretic pattern; and a trigger device, which is arranged corresponding to the frame; wherein the trigger device can be used to trigger the cell outer layer of the target biological particle located between the two working sections, so that the cell outer layer has a predetermined permeability greater than the initial permeability, thereby causing the target biological particle to proliferate an exosome that passes through the cell outer layer; When the target biological particles are captured between the two working sections and the exosomes proliferate, the light capturing device can be used to form a second dielectrophoretic pattern on the light sensing structure, so as to drive the exosomes through the second opening between the two working sections and leave the two working sections through the second dielectrophoretic pattern to achieve a purification operation.

11. The non-contact biological particle processing device according to claim 10, characterized in that: The area between the two working sections is formed by: a receiving area, one end of which is defined with the first opening; and A necking region is located at the other end of the receiving region; wherein the necking region is gradually tapered in a direction away from the receiving region, and the necking region defines the second opening away from the first opening.

12. The non-contact biological particle processing device according to claim 11, characterized in that: The trigger device comprises: Two electrode pads, located in the receiving area and respectively arranged on the inner walls of the two working sections; and A power source is electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the containment area, the power source can drive the two electrode pads to apply an electric field to the target biological particles so that the target biological particles form an electroporation, thereby making the cell surface layer have the predetermined permeability.

13. The non-contact biological particle processing device according to claim 11, characterized in that: The trigger device comprises: Two electrode pads, located in the necking area and respectively arranged on the inner walls of the two working sections; and A power source is electrically coupled to the two electrode pads; wherein, when the target biological particle is captured in the necking area, the power source can drive the two electrode pads to apply an electric field to the target biological particle so that the target biological particle forms an electroporation, thereby making the cell surface layer have the predetermined permeability.

14. The non-contact biological particle processing device according to claim 10, characterized in that: The frame includes two channel sections, and the two channel sections are respectively connected to the two working section parts defining the second opening; wherein the two channel sections define a third opening away from the second opening, which is larger than the second opening.

15. A non-contact biological particle processing device, characterized in that: The non-contact biological particle processing equipment comprises: A containing device, which is used to contain a liquid specimen, which contains a plurality of biological particles, and the containing device comprises: A light sensing structure comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure, spaced apart from the light sensing structure; wherein at least one of the light sensing structure and the matching structure is transparent, and the matching structure comprises a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light sensing structure; and a frame disposed between the light sensing structure and the matching structure; wherein the frame has two working sections facing each other, and the opposite ends of the frame are respectively formed with a first opening and a second opening smaller than the first opening; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; and A trigger device is arranged corresponding to the frame; wherein, when the target biological particle passes through the first opening and enters between the two working sections, the trigger device can be used to trigger the cell surface layer of the target biological particle so that the cell surface layer has a predetermined permeability greater than the initial permeability.

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