Special microbial growth regulator for bananas and application of special microbial growth regulator

Abstract

The invention belongs to the field of biology, and particularly relates to a microbial growth regulator special for bananas and application of the microbial growth regulator. The special microbial growth regulator for the bananas is prepared from the following components in parts by weight: 1 to 3 parts of streptomyces coronamycin, 0.5 to 1.5 parts of trichoderma harzianum, 0.5 to 1.5 parts of saccharomycetes, 10 to 20 parts of guava leaves and 10 to 20 parts of bagasse, the streptomyces coronamycin is streptomyces coronamycin s-24, and the preservation number of the streptomyces coronamycin is CCTCC No: M 2017353. The components are mixed, fermented and applied to a banana garden, so that the root length of banana plants can be increased, the cauloid thickness of the banana plants is effectively increased, the toughness of the banana plants is improved, the wind resistance of the banana plants is improved, the loss of the banana garden caused by typhoon can be effectively reduced, and a new way is provided for green cultivation of bananas.

Description

Technical Field

[0001] The invention belongs to the biological field, and in particular relates to improving a special microbial growth regulator for banana and application thereof. Background Art

[0002] Banana (scientific name: Musa nana Lour.) is a plant of the genus Musa in the family Musaceae. It also refers to its fruit, which is widely cultivated and eaten in tropical areas. Banana leaves are large and shallow, pseudostems are loose and fleshy, and the fruit clusters are heavy. Typhoons often occur in tropical and subtropical areas. Once encountered, banana plants are very likely to break and fall, causing heavy losses to banana plantations. Therefore, in the process of banana cultivation, it is of great significance to enhance the wind resistance of banana plants, especially through daily management measures such as water and fertilizer management, to enhance the wind resistance of banana plants. Summary of the invention

[0003] The invention provides a special microbial growth regulator for banana and application thereof, which can promote the growth of the root system of banana plants, effectively increase the thickness of the pseudostem of banana plants, and help improve the wind resistance of banana plants.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A microbial growth regulator for bananas comprises the following components in parts by weight: 1-3 parts of Streptomyces chrestomyceticus, 0.5-1.5 parts of Trichoderma harzianum, 0.5-1.5 parts of yeast, 10-20 parts of guava leaves, and 10-20 parts of bagasse. The Streptomyces chrestomyceticus is Streptomyces chrestomyceticus s-24, and its preservation number is CCTCC No: M 2017353.

[0006] Furthermore, the banana-specific microbial growth regulator comprises the following components in parts by weight: 2 parts of Streptomyces coronarius, 1 part of Trichoderma harzianum, 1 part of yeast, 15 parts of guava leaves, and 15 parts of sugarcane bagasse.

[0007] Furthermore, the total number of viable bacteria of the Streptomyces coronaeum is ≥ 10 7 cfu / mL; the total number of viable bacteria of Trichoderma harzianum is ≥10 7 cfu / mL; total number of viable yeast cells ≥10 7 cfu / mL.

[0008] Furthermore, the Trichoderma harzianum is Trichoderma harzianum T-22.

[0009] A method for preparing a microbial growth regulator for bananas comprises the following steps:

[0010] (1) Take dried guava leaves and sugarcane bagasse, crush them, add water and stir evenly;

[0011] (2) Add Streptomyces coronatus, Trichoderma harzianum and yeast to the stirred materials, stir evenly, and then ferment them in a pile to obtain the product.

[0012] Furthermore, in step (1), 40-50% of the total mass of guava leaves and bagasse is added with water.

[0013] Furthermore, in step (2), the compost fermentation time is 7-15 days; during the fermentation process, the temperature is maintained at 20-35°C, and the compost is turned over to cool down when the temperature exceeds 35°C.

[0014] The application of the above-mentioned banana-specific microbial growth regulator in the preparation of a preparation for promoting root growth of banana plants.

[0015] Furthermore, in the application, the banana-specific microbial growth regulator can increase the root length of banana plants.

[0016] The above-mentioned banana-specific microbial growth regulator is used in the preparation of a preparation for increasing the pseudostem thickness of banana plants.

[0017] Furthermore, in the application, the banana-specific microbial growth regulator is applied starting from the third month after banana transplanting; 1-2 times a month; the application amount per mu is 15-20 kg, and it can be directly buried in the soil around the plant during application.

[0018] Beneficial effects of the present invention:

[0019] The banana-specific microbial growth regulator of the present invention can promote the root growth rate of banana plants, increase the root length of banana plants, effectively increase the pseudostem thickness of banana plants, improve the toughness of banana plants, help improve the wind resistance of banana plants, effectively reduce the loss of banana plantations caused by typhoons, and provide a new way for the green cultivation of bananas. DETAILED DESCRIPTION

[0020] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The Streptomyces Chrestomyceticus s-24 described in the present invention has a deposit number of CCTCC No: M 2017353, a deposit date of June 19, 2017, and a deposit unit of the China Center for Type Culture Collection, with an address of Wuhan University, Wuhan, China.

[0022] Example 1

[0023] A microbial growth regulator for bananas, comprising the following components in parts by weight: 1 part of Streptomyces chrestomyceticus, 0.5 part of Trichoderma harzianum, 0.5 part of yeast, 10 parts of guava leaves, and 10 parts of sugarcane bagasse, wherein the Streptomyces chrestomyceticus is Streptomyces chrestomyceticus s-24, and its deposit number is CCTCC No: M 2017353. The total number of viable bacteria of the Streptomyces chrestomyceticus is 10 7 cfu / mL; the total number of viable bacteria of Trichoderma harzianum is 10 7 cfu / mL; the total number of viable yeast cells is 10 7 cfu / mL.

[0024] A method for preparing a microbial growth regulator for bananas comprises the following steps:

[0025] (1) Take dried guava leaves and sugarcane bagasse, crush them, add 40-50% of the total weight of guava leaves and sugarcane bagasse into water and stir evenly;

[0026] (2) Add Streptomyces coronatus, Trichoderma harzianum and yeast to the stirred materials, stir evenly, and ferment for 7-15 days. During the fermentation process, maintain the temperature at 20-35°C. Turn the pile to cool down when the temperature exceeds 35°C.

[0027] Example 2

[0028] A microbial growth regulator for bananas, comprising the following components in parts by weight: 2 parts of Streptomyces chrestomyceticus, 1 part of Trichoderma harzianum, 1 part of yeast, 15 parts of guava leaves, and 15 parts of sugarcane bagasse. The Streptomyces chrestomyceticus is Streptomyces chrestomyceticus s-24, and its preservation number is CCTCC No: M 2017353. The total number of viable bacteria of the Streptomyces chrestomyceticus is 10 8 cfu / mL; the total number of viable bacteria of Trichoderma harzianum is 10 9 cfu / mL; the total number of viable yeast cells is 10 8 cfu / mL.

[0029] The preparation method is the same as that in Example 1.

[0030] Example 3

[0031] A microbial growth regulator for bananas, comprising the following components in parts by weight: 3 parts of Streptomyces chrestomyceticus, 1.5 parts of Trichoderma harzianum, 1.5 parts of yeast, 20 parts of guava leaves, and 20 parts of sugarcane bagasse. The Streptomyces chrestomyceticus is Streptomyces chrestomyceticus s-24, and its preservation number is CCTCC No: M 2017353. The total number of viable bacteria of the Streptomyces chrestomyceticus is 10 10 cfu / mL; the total number of viable bacteria of Trichoderma harzianum is 10 10 cfu / mL; the total number of viable yeast cells is 10 9 cfu / mL.

[0032] The preparation method is the same as that in Example 1.

[0033] In the above examples, the Trichoderma harzianum used is the Trichoderma harzianum T-22 strain, which can be purchased directly from the market. The yeast used is Angel Yeast commonly used in the market for fermentation.

[0034] The above-mentioned banana-specific microbial growth regulator can be applied in the third month after banana transplanting, with an application rate of 15-20 kg per mu, 1-2 times per month. When applying, it can be directly buried in the soil around the banana plants.

[0035] Effect of the banana-specific microbial growth regulator of the present invention on the growth of banana plants

[0036] In order to facilitate comparison of experimental results, the present invention selects banana seedlings from the same batch of Brazilian banana tissue culture seedlings, that is, the number of leaves is 5-7, the pseudostem thickness is ≥0.9cm, the leaf width is ≥6.8cm, the leaf color is green and not elongated, the leaves have no disease spots and no pests; the root system grows well; there is no mechanical damage, and the mutation rate is ≤0.01%. Uniformly select plots that have not been planted with bananas in the past three years for banana cultivation experiments, plant banana seedlings at a density of 150 seedlings per mu, and each treatment has 100 seedlings to compare the effects of growth regulators of different compositions on the growth of banana plants. The specific experimental method is as follows. The composition of the growth regulator used is shown in Table 1. Its preparation method refers to Example 1.

[0037] Table 1 Composition of growth regulators used in different treatment groups

[0038]

[0039]

[0040] From the third month after transplanting banana seedlings, different growth regulators were applied, with an application rate of 20 kg per mu, twice a month. When applying, bury directly into the soil around the plants. It is best to keep the fertilizer application 0.5-1 meter away from the banana plants, and try to avoid damaging the roots due to soil tillage. Daily field management was carried out uniformly. After continuous application for 3 months, the effects on the root length and pseudostem diameter of banana plants were statistically analyzed. See Table 2 for details.

[0041] The measurement position of the banana plant pseudostem diameter was unified as the middle diameter of the pseudostem.

[0042] Table 2 Effects of different treatments on banana root system and stem diameter

[0043] Serial number Root length (cm) Pseudostem diameter (cm) 1 57.31 22.76 2 58.26 23.69 3 58.05 23.18 4 40.12 15.04 5 39.07 15.91 6 38.94 14.39 7 41.20 13.21 8 40.35 14.46

[0044] It can be seen from Table 2 that the growth regulators prepared by the compositions and preparation methods of the three embodiments of the present invention in treatment groups 1-3 can significantly increase the root length and pseudostem diameter of banana.

[0045] Treatment groups 4-7 changed the composition of the microbial agent in the growth regulator. Although treatment group 7 had the same composition, it was not fermented. Although guava leaves and sugarcane bagasse were composted in treatment group 8, no microbial agent was added. The root length and stem diameter of the banana plants in the above treatment groups were reduced to varying degrees, and were significantly lower than those in the three example groups. It is shown that the growth regulator prepared by fermenting guava leaves and sugarcane bagasse with a combination of Streptomyces coronarius, Trichoderma harzianum and yeast in the three example groups of the present invention can significantly increase the root length and pseudostem diameter of banana plants, and its effect is significantly improved compared with other treatment groups.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial growth regulator for banana, characterized in that: The invention comprises the following components in parts by weight: 1-3 parts of Streptomyces chrestomyceticus, 0.5-1.5 parts of Trichoderma harzianum, 0.5-1.5 parts of yeast, 10-20 parts of guava leaves and 10-20 parts of sugarcane bagasse. The Streptomyces chrestomyceticus is Streptomyces chrestomyceticus s-24, and its preservation number is CCTCC No: M 2017353.

2. A microbial growth regulator for banana as claimed in claim 1, characterized in that: The microbial growth regulator for bananas comprises the following components in parts by weight: 2 parts of Streptomyces coronarius, 1 part of Trichoderma harzianum, 1 part of yeast, 15 parts of guava leaves and 15 parts of sugarcane bagasse.

3. A microbial growth regulator for banana according to claim 1 or 2, characterized in that: The total number of viable bacteria of the Streptomyces coronaeum is ≥ 10 7 cfu / mL; the total number of viable bacteria of Trichoderma harzianum is ≥10 7 cfu / mL; total number of viable yeast cells ≥10 7 cfu / mL.

4. A microbial growth regulator for banana according to claim 1 or 2, characterized in that: The Trichoderma harzianum is Trichoderma harzianum T-22.

5. The preparation method of a special microbial growth regulator for banana as claimed in claim 1 or 2, characterized in that, The following steps are involved: (1) Take dried guava leaves and sugarcane bagasse, crush them, add water and stir evenly; (2) Add Streptomyces coronatus, Trichoderma harzianum and yeast to the stirred materials, stir evenly, and then ferment them in a pile to obtain the product.

6. The preparation method according to claim 5, characterized in that: In step (1), 40-50% of the total mass of guava leaves and bagasse is added with water; in step (2), the composting and fermentation time is 7-15 days; during the fermentation process, the temperature is maintained at 20-35° C., and the compost is turned over to cool down when the temperature exceeds 35° C.

7. Application of the special microbial growth regulator for banana as claimed in claim 1 or 2 in the preparation of a preparation for promoting root growth of banana plants.

8. The use according to claim 7, characterized in that The special microbial growth regulator for bananas can increase the root length of banana plants.

9. Use of the banana-specific microbial growth regulator as claimed in claim 1 or 2 in preparing a preparation for increasing the pseudostem thickness of banana plants.

10. The use according to claim 8 or 9, characterized in that: The banana-specific microbial growth regulator is applied starting from the third month after banana transplanting, and is applied 1-2 times a month; the application amount per mu is 15-20 kg, and the agent can be directly buried in the soil around the plant during application.

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