Dry-type detection and analysis assembly for inorganic phosphorus

Abstract

The invention discloses an inorganic phosphorus dry type detection analysis assembly, the assembly sequentially comprises an upper support layer, a diffusion layer, a first reagent layer, a second reagent layer, a support body and a lower support layer from top to bottom, the two ends of the upper support layer and the lower support layer are respectively connected through buckles, and a sample adding hole is formed in the middle position of the upper support layer; a test hole is formed in the middle of the lower supporting layer; reagents contained in the diffusion layer comprise 5-sulfosalicylic acid, succinic acid, a non-ionic high-molecular chemical substance and triton X-100; reagents contained in the first reagent layer comprise a modified 30% binder, a modified acrylic resin emulsion, white glue, ammonium molybdate, ascorbic acid, a surfactant, soluble cellulose ether, a defoaming agent and an active material; a reagent contained in the second reagent layer comprises D4 hydrogel with the concentration of 0.3 g / L. According to the assembly, the problem of multi-layer coating in the prior art is greatly solved, the permeation effect is improved, and the purposes of normal-temperature storage, coating frequency simplification, process simplification and production cost reduction are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro detection, and in particular to an inorganic phosphorus dry-type detection and analysis component. Background Art

[0002] Phosphorus in the blood includes inorganic phosphorus and organic phosphorus. The blood phosphorus detected clinically is serum inorganic phosphorus, which participates in the metabolism of sugar, lipid and amino acid, and regulates acid-base balance. Serum inorganic phosphorus is of great significance for the diagnosis and treatment of bone diseases, thyroid diseases, parathyroid diseases, chronic kidney disease, electrolyte disorders, etc.

[0003] At present, the commonly used dry analysis components for detecting inorganic phosphorus are to coat the shielding layer, diffusion layer, reagent layer, color development layer, etc. on a transparent support in sequence according to different functions, and detect the concentration of the object to be tested by measuring the change of reflected light. This type of multilayer film dry analysis component has accurate test results and is easy to operate, but its production process is complicated and the production cost is high. This is because: its production process requires multi-layer coating, shielding light source and dispersing samples to make the sample diffuse evenly; the prepared analysis component test area needs to be large to meet the requirements of subsequent testing by reflectance photometry (Kubelka-Munk theory), avoiding the problem of weak light receiving signal and insufficient sensitivity caused by small test area; the prepared component needs to be stored at low temperature, because the storage condition of the raw material fish gelatin used in the production process is 2-8 ° C, and the gel effect of fish gelatin is poor at room temperature. In addition, the organic solvents used in the production process (such as using organic solvents to prepare diffusion layers, etc.) have high requirements on materials and equipment, and there is a risk of endangering the health of operators and polluting the environment. Therefore, under the premise of ensuring the accuracy of the test results, a dry analysis component of inorganic phosphorus with a simpler preparation process and lower cost is needed. Summary of the invention

[0004] In order to solve the above problems, the present invention provides an inorganic phosphorus dry detection and analysis component.

[0005] The present invention adopts the following technical solutions:

[0006] An inorganic phosphorus dry detection and analysis component comprises, from top to bottom, an upper support layer, a diffusion layer, a first reagent layer, a second reagent layer, a support and a lower support layer, wherein the upper support layer and the lower support layer are connected at both ends by snap fasteners, a sample addition hole is arranged in the middle of the upper support layer, and a test hole is arranged in the middle of the lower support layer; the reagents contained in the diffusion layer include 5-sulfosalicylic acid, succinic acid, non-ionic polymer chemicals and Triton X-100; the reagents contained in the first reagent layer include modified 30% binder, modified acrylic resin emulsion, white glue, ammonium molybdate, ascorbic acid, surfactant, soluble cellulose ether, defoaming agent and active material; the reagents contained in the second reagent layer include D4 hydrogel with a concentration of 0.3 g / L.

[0007] Furthermore, the diffusion layer is made of a mesh with an opening ratio of 400 or 500;

[0008] The first reagent layer and the second reagent layer are transparent plastic substrates or frosted plastic substrates with a light transmittance of more than 80% under a wavelength of 300-800nm, including any one of polyethylene terephthalate, polycarbonate, polypropylene, polyethylene, and polycarbonate sheets, and the thickness thereof is 30-200μm;

[0009] The support body is made of PET material with a thickness of 100-200 um, including any one of polyethylene terephthalate, polybutylene terephthalate, and polyvinyl chloride.

[0010] Furthermore, in the diffusion layer, the 5-sulfosalicylic acid is added in an amount of 1-20 g per unit square meter or 1-15 g per unit square meter; the succinic acid is added in an amount of 1-20 g per unit square meter or 1-7 g per unit square meter; the non-ionic polymer chemical includes any one or more of PVPK90, PVPK30, PVPK15, and PVPK17 and is added in an amount of 1-15 g per unit square meter or 1-10 g per unit square meter; the Triton X-100 is added in an amount of 1-10 g per unit square meter or 1-8 g per unit square meter.

[0011] Further, the modified 30% binder is added to the first reagent layer in an amount of 1-50 g per unit square meter or 1-40 g per unit square meter;

[0012] The preparation method of the modified 30% binder is as follows:

[0013] S1. Weigh acrylamide and N-vinyl pyrrolidone in a mass ratio of 8:2 and dissolve them in 100 g of ultrapure water. After dissolution, weigh 2% of N,N'-methylenebisacrylamide and 1% of N,N,N',N'-tetramethylethylenediamine and stir them evenly in the solution.

[0014] S2, transferring the solution obtained in step S1 to a polymerization reactor, introducing nitrogen for 10 min, raising the temperature to 40° C. after the nitrogen is introduced, and maintaining the temperature for 30 min under the protection of nitrogen;

[0015] S3. Add 0.4% potassium persulfate and sodium bisulfite, continue to react at a constant temperature for 10 minutes, then react for 4 hours under sealed conditions, take out, and test the viscosity with a viscometer. The binder that passes the test is the desired binder.

[0016] Furthermore, the modified acrylic resin emulsion is added to the first reagent layer in an amount of 1-30g per unit square meter or 2-20g per unit square meter; the white glue is added to the first reagent layer in an amount of 0.05-30g per unit square meter or 0.1-20g per unit square meter; the ammonium molybdate is added to the first reagent layer in an amount of 0.05-3g per unit square meter or 0.1-2g per unit square meter; the ascorbic acid is added to the first reagent layer in an amount of 0.05-10g per unit square meter or 0.1-7g per unit square meter.

[0017] Further, the surfactant is added to the first reagent layer in an amount of 0.05-5 g per unit square meter or in an amount of 0.07-2 g per unit square meter;

[0018] The surfactant is a zwitterionic surfactant, a cationic surfactant or an anionic surfactant; the zwitterionic surfactant includes any one of Tween 20, Tween 80, Triton X-405 and Triton X-100; the cationic surfactant includes any one of hexadecyltrimethylammonium bromide and dodecyltrimethylammonium bromide; the anionic surfactant includes any one or more of sodium dodecylbenzene sulfonate and sodium dodecyl sulfate.

[0019] Furthermore, the soluble cellulose ether is added to the first reagent layer in an amount of 1-30 g per unit square meter or 1-20 g per unit square meter; the soluble cellulose ether includes any one of methyl cellulose, hydroxyethyl methyl cellulose, carboxymethyl cellulose, ethyl cellulose, benzyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, cyanoethyl cellulose, benzyl cyanoethyl cellulose, carboxymethyl hydroxyethyl cellulose and phenyl cellulose.

[0020] Furthermore, the defoamer is added to the first reagent layer in an amount of 0.1-5 ml per unit square meter or 0.5-2 ml per unit square meter; the defoamer includes any one or more of silicone defoamers, polyether defoamers, and composite defoamers.

[0021] Furthermore, the active material is added to the first reagent layer in an amount of 1-50 g per unit square meter or 10-40 g per unit square meter; the active material includes any one or more of diatomaceous earth, volcanic ash, silica gel, fly ash, microcrystalline cellulose, bentonite, titanium dioxide, burnt clay, and cellulose powder.

[0022] Furthermore, the preparation method of the component is as follows:

[0023] A1. Solution preparation of the second reagent layer: weigh 30 g of D4 hydrogel and dissolve it in 100 ml of mixed alcohol, where the ratio of alcohol to water is 9:1. Wait until it is completely dissolved to obtain the solution of the second reagent layer.

[0024] A2. Solution preparation of the first reagent layer: weigh white glue, ammonium molybdate, and ascorbic acid respectively and add them to the aqueous solution in sequence. After they are completely dissolved, add active materials, modified acrylic resin emulsion, modified 30% binder, surfactant, and soluble cellulose ether to the solution in sequence. The stirring speed is controlled at 500 r / min and the stirring time is 2 h.

[0025] A3. Continue to add defoamer to step A2. After adding, adjust the stirring speed from 500r / min to 400r / min. The stirring time is 1h.

[0026] A4, use a heated doctor blade coater to coat the second reagent layer solution of 0.3g / ml D4 hydrogel on the PET substrate, the coating wet film thickness is 100um, after coating is completed, transfer to a 60°C oven for drying for 5 minutes, and after drying, transfer to a tray for standby use;

[0027] A5, the first reagent layer slurry that has been stirred evenly in step A3 is moved onto the coated second reagent layer for a second coating, the coating wet film thickness is controlled to be 100-500 μm, and dried at a temperature range of 50°C-70°C, and the dry film thickness after drying is 50-200 μm;

[0028] A6. Cut the first reagent layer, the second reagent layer and the diffusion layer gauze dried in step A5 into 9.5*9.5mm 2 The small pieces are fixed and assembled into inorganic phosphorus dry detection and analysis components using customized black PET plastic card sleeves as the upper and lower support layers.

[0029] After adopting the above technical solution, the present invention has the following advantages compared with the background technology:

[0030] 1. The present invention optimizes and innovates the formula, coating process and reagent cost. The first reagent layer is combined with a modified 30% binder and a modified acrylic resin emulsion, and connected with an active material to prepare a reagent layer with fast diffusion, penetration and uniform color development. The inorganic phosphorus dry detection and analysis component of the present invention only needs to be coated with two layers (the first reagent layer and the second reagent layer), which greatly improves the problem of multi-layer coating in the prior art and improves the penetration effect, realizes the purpose of room temperature storage, simplifies the number of coatings, simplifies the process, and reduces the production cost. The test results are highly accurate and have a wide measurement range, meeting the needs of instant diagnosis and on-site diagnosis, and can be applied to clinical value;

[0031] 2. In addition, the present invention uses gauze as the diffusion layer, which is environmentally friendly and safe, and can achieve rapid penetration and accurate detection of samples to be analyzed containing inorganic phosphorus, solving the problems of organic reagent pollution of the diffusion layer and harm to the human body and the environment in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a structural schematic diagram of the components of the present invention;

[0033] Figure 2 is a linear correlation diagram of the component test results of Example 3 and the biochemical analyzer test results;

[0034] Figure 3 is a linear correlation diagram of the component test results of Example 4 and the biochemical analyzer test results;

[0035] Figure 4 It is a linear correlation diagram of the component test results of Example 5 and the biochemical analyzer test results.

[0036] Description of reference numerals:

[0037] 1. Upper support layer; 2. Diffusion layer; 3. First reagent layer; 4. Second reagent layer; 5. Support; 6. Lower support layer; 7. First card position; 8. Second card position; a. Sample loading hole; b. Test hole. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0039] Example 1

[0040] like Figure 1As shown, an inorganic phosphorus dry detection and analysis component comprises, from top to bottom, an upper support layer 1, a diffusion layer 2, a first reagent layer 3, a second reagent layer 4, a support body 5 and a lower support layer 6. The upper support layer 1 and the lower support layer 6 are respectively connected at both ends by a first clamping position 7 and a second clamping position 8, which play the role of fixing and protecting the diffusion layer 2, the first reagent layer 3, the second reagent layer 4, and the support body 5; a sample addition hole a is provided in the middle of the upper support layer 1, and a test hole b is provided in the middle of the lower support layer 6; The body 5 plays a supporting role, and the reagents contained in the diffusion layer 2 include 5-sulfosalicylic acid, succinic acid, non-ionic polymer chemicals and Triton X-100; the reagents contained in the first reagent layer 3 include modified 30% binder (i.e. poly(acrylamide-N-vinyl pyrrolidone)), modified acrylic resin emulsion, white glue, ammonium molybdate, ascorbic acid, surfactant, soluble cellulose ether, defoaming agent and active material; the reagents contained in the second reagent layer 4 include D4 hydrogel with a concentration of 0.3g / L.

[0041] Specifically, the diffusion layer 2 uses a gauze with an opening rate of 400; in the diffusion layer 2, the 5-sulfosalicylic acid is added in an amount of 10 g per unit square meter; the succinic acid is added in an amount of 5 g per unit square meter; the non-ionic polymer chemical uses PVPK90 and is added in an amount of 8 g per unit square meter; the Triton X-100 is added in an amount of 6 g per unit square meter.

[0042] The first reagent layer 3 and the second reagent layer 4 are made of a transparent plastic substrate polyethylene terephthalate with a light transmittance of more than 80% under a wavelength of 500 nm, and a thickness of 100 μm;

[0043] Among them, in the first reagent layer 3, the modified 30% binder is added in an amount of 30g per unit square meter; the modified acrylic resin emulsion (purchased directly, with the advantages of good film-forming property, strong adhesion, good shielding property, fast drying speed, and green environmental protection) is added in an amount of 15g per unit square meter; the white glue is added in an amount of 10g per unit square meter; the ammonium molybdate is added in an amount of 0.3g per unit square meter; the ascorbic acid is added in an amount of 0.3g per unit square meter; the surfactant adopts the zwitterionic surfactant Tween 20 and is added in an amount of 0.1g per unit square meter; the soluble cellulose ether methyl cellulose is added in an amount of 15g per unit square meter; the defoaming agent polyether defoaming agent is added in an amount of 0.7ml per unit square meter; the active material diatomaceous earth is added in an amount of 30g per unit square meter.

[0044] The support 5 is made of PET material polyethylene terephthalate with a thickness of 100 um.

[0045] The preparation method of the modified 30% binder is as follows:

[0046] S1. Weigh acrylamide and N-vinyl pyrrolidone in a mass ratio of 8:2 and dissolve them in 100 g of ultrapure water. After dissolution, weigh 2% of N,N'-methylenebisacrylamide and 1% of N,N,N',N'-tetramethylethylenediamine and stir them evenly in the solution.

[0047] S2, transferring the solution obtained in step S1 to a polymerization reactor, introducing nitrogen for 10 min (to remove oxygen in the solution), raising the temperature to 40° C. after the nitrogen is introduced, and maintaining the temperature for 30 min under the protection of nitrogen;

[0048] S3. Add 0.4% potassium persulfate and sodium bisulfite, continue to react at a constant temperature for 10 minutes, then react for 4 hours under sealed conditions, take out, and test the viscosity with a viscometer. The binder that passes the test is the desired binder.

[0049] The detection method of the inorganic phosphorus dry detection and analysis component of the present invention is as follows:

[0050] 10ul of the serum sample to be tested is vertically dripped into the sample well a. The sample diffuses to the diffusion layer 2, the first reagent layer 3 and the second reagent layer 4 in turn, and is incubated at about 37°C for 300s. During this process, the inorganic phosphorus and ammonium molybdate in the serum produce phosphomolybdic acid under acidic conditions. The phosphomolybdic acid generates a blue complex under the action of the ascorbic acid reducing agent. This complex is measured for reflected light at a wavelength of 610nm. The change in reflectivity is measured according to the reflectivity, and the concentration of inorganic phosphorus is calculated by the change in reflectivity. The corresponding substance reactions of the inorganic phosphorus dry analytical reagent are as follows:

[0051] (1) Inorganic phosphorus + ammonium molybdate generates phosphomolybdic acid under acidic conditions;

[0052] (2) Phosphomolybdic acid + ascorbic acid forms a blue complex.

[0053] The dry analytical reagent for detecting inorganic phosphorus of the present invention has good stability, is easy to store and preserve, has high detection accuracy and a wide linear range.

[0054] Example 2

[0055] This embodiment specifically introduces the preparation method of the component as follows:

[0056] A1. Solution preparation of the second reagent layer: weigh 30 g of D4 hydrogel and dissolve it in 100 ml of mixed alcohol, where the ratio of alcohol to water is 9:1. Wait until it is completely dissolved to obtain the solution of the second reagent layer.

[0057] A2. Solution preparation of the first reagent layer: white glue, ammonium molybdate, and ascorbic acid were weighed and added to the aqueous solution in sequence. After they were completely dissolved, active materials, modified acrylic resin emulsion, modified 30% binder, surfactant, and soluble cellulose ether were added to the solution in sequence. The stirring speed was controlled at 500 r / min and the stirring time was 2 h. In this process, the stirrer used was an electric stirrer of model MYP2011-150.

[0058] A3. Continue to add defoamer to step A2. After adding, adjust the stirring speed from 500r / min to 400r / min. The stirring time is 1h.

[0059] A4, use a heated blade coater model PW-TB100 to coat the second reagent layer solution of 0.3g / ml D4 hydrogel on the PET substrate, the wet film thickness of the coating is 100um, the coating speed is 50cm / min, the coating temperature is controlled at 27°C, after coating is completed, transfer to a 60°C oven for drying, the drying time is 5min, and after drying, transfer to a tray for standby use;

[0060] A5, the first reagent layer slurry stirred evenly in step A3 is transferred to the coated second reagent layer for a second coating, the coating machine used is a heating scraper coater of model PW-TB100, the coating wet film thickness is controlled at 100-500 μm, and the coating is dried at a temperature range of 50°C-70°C, and the dry film thickness after drying is 50-200 μm;

[0061] A6. Cut the first reagent layer, the second reagent layer and the diffusion layer gauze dried in step A5 into 9.5*9.5mm 2 The small pieces are fixed and assembled into inorganic phosphorus dry detection and analysis components using customized black PET plastic card sleeves as the upper and lower support layers.

[0062] The diffusion layer solution is prepared by weighing 5-sulfosalicylic acid, succinic acid, non-ionic polymer compounds and Triton X-100 into the aqueous solution, stirring them evenly, and using the scraping process to immerse the gauze with an opening rate of 400 or 500, scrape and dry it. The drying temperature is controlled at 60°C and the drying time is controlled at 10-15 minutes.

[0063] Example 3

[0064] This embodiment specifically introduces the preparation and test results of an inorganic phosphorus dry detection and analysis component as follows:

[0065] Preparation of the second reagent layer: Weigh 30 g of D4 hydrogel into 100 ml of mixed alcohol (wherein the ratio of alcohol to water is 9:1) and wait for dissolution before use.

[0066] Preparation of the first reagent layer: weigh a certain mass of ultrapure water, add the white glue, ammonium molybdate, and VC in the reagent group to the solution in turn, and add active materials, modified acrylic resin emulsion, modified 30% binder, surfactants, and soluble cellulose ether in turn after the solution is completely dissolved. Stir with a spiral stirrer, control the speed at 600r / min, stir for 2h, and continue to add defoamer to the slurry when the time is up. At this time, the speed is adjusted from 600r / min to 400r / min, and stir for 1h before coating. Preparation of the diffusion layer: weigh 5-sulfosalicylic acid, succinic acid, PVPK90, and Triton X-100 in the aqueous solution in turn until dissolved and stirred evenly. When the solution is clear and transparent, use the scraping process technology to soak, scrape and dry the gauze with an opening rate of 400 or 500. The drying temperature is controlled at 60℃ and the drying time is controlled at 10-15min.

[0067] Place the transparent 125um thick PET film support on the coating machine, press both ends, return the micrometer to zero, set the coating machine wet film thickness to 90um, transfer the D4 hydrogel of the second reagent layer to the bottom of the scraper mouth, press the coating button, the scraper starts coating, and the coating speed is controlled at 50cm / min. Place the coated second reagent layer in a 50℃ oven for constant temperature drying. The drying time is 5min. After drying, place it back on the coating machine, set the coating machine wet film thickness to 350um, pour the slurry of the first reagent layer to the bottom of the scraper mouth, and press the coating button to start coating. Put the coated first reagent layer into a 50℃ oven for drying, and the drying time is 20min. The reagent layer to be dried is cut with a long strip cutter, and the cutting specification is 294*9.5mm. The gauze was cut into the same size, and then cut into small pieces of 9.5*9.5mm2. Finally, a black PET card sleeve was used as the upper support layer, the lower support layer and the support body to fix and assemble them into a dry detection and analysis component, and then used for measurement.

[0068] Table 1 Formula of the first reagent layer in Example 3

[0069] Reagents Coating amount / m2 Ammonium molybdate 1g 10% white glue 10ml Hexadecyltrimethylammonium bromide 300mg Modified acrylic resin emulsion 12g Modified 30% Binder 18g Silicone defoamer 1g Hydroxyethyl Cellulose 10g diatomite 30g Fly ash 5g VC 5g

[0070] Table 2 Diffusion layer formulation in Example 3

[0071] Reagents Coating amount / m2 5-Sulfosalicylic acid 8.5g Succinic acid 1.5g PVPk90 3g Triton X-100 5ml

[0072] 25 serum samples of different gradient concentrations were prepared in proportion by Randox quality control, and the correlation of multiple test samples was analyzed by the method of this embodiment and the biochemical analyzer test method. The results are as follows: Figure 2 As shown, the test results of the method of this embodiment and the test results of the biochemical analyzer method show a good linear correlation.

[0073] Example 4

[0074] This embodiment specifically introduces the preparation and test results of an inorganic phosphorus dry detection and analysis component as follows:

[0075] Preparation of the second reagent layer: Weigh 30 g of D4 hydrogel into 100 ml of mixed alcohol (wherein the ratio of alcohol to water is 9:1) and wait for dissolution before use.

[0076] Preparation of the first reagent layer: Weigh a certain mass of ultrapure water, add the white glue, ammonium molybdate, and VC in the reagent group to the solution in turn, and add active materials, modified acrylic resin emulsion, modified 30% binder, surfactants, and soluble cellulose ether in turn after the solution is completely dissolved. Stir with a spiral stirrer, control the speed at 600r / min, stir for 2h, and continue to add defoamer to the slurry when the time is up. At this time, the speed is adjusted from 400r / min to 500r / min, and stir for 1h before coating. Preparation of the diffusion layer: Weigh 5-sulfosalicylic acid, succinic acid, pvpk30, and Triton X-100 in the aqueous solution in turn until dissolved and stirred evenly. When the solution is clear and transparent, use the scraping process technology to soak, scrape and dry the gauze with an opening rate of 400 or 500. The drying temperature is controlled at 60℃ and the drying time is controlled at 10-15min.

[0077] Place the transparent PET film support on the coating machine, press both ends, return the micrometer to zero, set the coating machine wet film thickness to 100um, transfer the D4 hydrogel of the second reagent layer to the bottom of the scraper mouth, press the coating button, the scraper starts coating, and the coating speed is controlled at 80cm / min. Place the coated second reagent layer in a 60℃ oven for constant temperature drying. The drying time is 5min. After drying, place it back on the coating machine, set the coating machine wet film thickness to 340um, pour the slurry of the first reagent layer to the bottom of the scraper mouth, and press the coating button to start coating. Put the coated first reagent layer into a 60℃ oven for drying, and the drying time is 15min. The reagent layer to be dried is cut with a long strip cutter, and the cutting specification is 294*9.5mm. The gauze was then cut into the same size, and then cut into small pieces of 9.5*9.5 mm2. Finally, a black PET card sleeve was used as the upper support layer, lower support layer and support body to fix and assemble into a dry detection and analysis component, and then used for measurement.

[0078] Table 3 Formula of the first reagent layer in Example 4

[0079] Reagents Coating amount / m2 Ammonium molybdate 1.0g 10% white glue 12ml Dodecyltrimethylammonium bromide 400mg Triton X-100 2g Modified acrylic resin emulsion 10g Modified 30% binder 20g Composite defoamer 2g Bentonite 7g Benzyl cellulose 12g diatomite 25g VC 5g

[0080] Table 4 Diffusion layer formulation in Example 4

[0081] Reagents Coating amount / m2 5-Sulfosalicylic acid 8.3g Succinic acid 1.7g PVPk30 5g Triton X-100 6ml

[0082] 25 serum samples of different gradient concentrations were prepared in proportion by Randox quality control, and the correlation of multiple test samples was analyzed by the method of the present invention and the biochemical analyzer test method. The results are as follows: Figure 3 As shown, the test results of the method of the present invention and the test results of the biochemical analyzer method show a good linear correlation.

[0083] Example 4

[0084] This embodiment specifically introduces the preparation and test results of an inorganic phosphorus dry detection and analysis component as follows:

[0085] Preparation of the second reagent layer: weigh 30g of D4 hydrogel in 100ml of mixed alcohol (the ratio of alcohol to water is 9:1) and wait for dissolution. Preparation of the first reagent layer: weigh a certain mass of ultrapure water, add white glue, ammonium molybdate, and VC reagent in the reagent group to the solution in turn, and add active materials, modified acrylic resin emulsion, modified 30% binder adhesive, surfactant, and soluble cellulose ether in turn after the solution is completely dissolved. Stir with a spiral stirrer, control the speed at 550r / min, stir for 2h, and continue to add defoamer to the slurry when the time is up. At this time, the speed is adjusted from 550r / min to 400r / min, and stir for 1h before coating. Preparation of the diffusion layer: weigh 5-sulfosalicylic acid, succinic acid, PVPK30, and Triton X-100 in the aqueous solution in turn until dissolved and stirred evenly. When the solution is clear and transparent, use the scraping process technology to soak, scrape and dry the gauze with an opening rate of 400 or 500. The drying temperature is controlled at 60°C and the drying time is controlled at 10-15 minutes.

[0086] Place the transparent 125um thick PET film support on the coating machine, press both ends, return the micrometer to zero, set the coating machine wet film thickness to 95um, transfer the D4 hydrogel of the second reagent layer to the bottom of the scraper mouth, press the coating button, the scraper starts coating, and the coating speed is controlled at 70cm / min. Place the coated second reagent layer in a 70℃ oven for constant temperature drying. The drying time is 5min. After drying, place it back on the coating machine, set the coating machine wet film thickness to 345um, pour the slurry of the first reagent layer to the bottom of the scraper mouth, and press the coating button to start coating. Put the coated first reagent layer into a 70℃ oven for drying, and the drying time is 10min. The reagent layer to be dried is cut with a long strip cutter, and the cutting specification is 294*9.5mm. The gauze was then cut into the same size, and then cut into small pieces of 9.5*9.5 mm2. Finally, a black PET card sleeve was used as the upper support layer, lower support layer and support body to fix and assemble into a dry detection and analysis component, and then used for measurement.

[0087] Table 5: Formula of the first reagent layer in Example 5

[0088]

[0089]

[0090] Table 6 Diffusion layer formulation in Example 5

[0091] Reagents Coating amount / m2 5-Sulfosalicylic acid 8g Succinic acid 2g PVPk30 5g Triton X-100 7ml

[0092] 25 serum samples of different gradient concentrations were prepared in proportion by Randox quality control, and the correlation of multiple test samples was analyzed by the method of the present invention and the biochemical analyzer test method. The results are as follows Figure 4 As shown, the test results of the method of the present invention and the test results of the biochemical analyzer method show a good linear correlation.

[0093] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. An inorganic phosphorus dry detection and analysis component, characterized in that: The component comprises an upper support layer, a diffusion layer, a first reagent layer, a second reagent layer, a support body and a lower support layer in order from top to bottom; the two ends of the upper support layer and the lower support layer are respectively connected by snaps; a sample loading hole is arranged in the middle of the upper support layer, and a test hole is arranged in the middle of the lower support layer; the reagents contained in the diffusion layer include 5-sulfosalicylic acid, succinic acid, non-ionic polymer chemicals and Triton X-100; the reagents contained in the first reagent layer include modified 30% binder, modified acrylic resin emulsion, white glue, ammonium molybdate, ascorbic acid, surfactant, soluble cellulose ether, defoaming agent and active material; the reagents contained in the second reagent layer include D4 hydrogel with a concentration of 0.3g / L.

2. An inorganic phosphorus dry detection and analysis component as claimed in claim 1, characterized in that: The diffusion layer is made of a mesh with an opening rate of 400 or 500; The first reagent layer and the second reagent layer are transparent plastic substrates or frosted plastic substrates with a light transmittance of more than 80% under a wavelength of 300-800nm, including any one of polyethylene terephthalate, polycarbonate, polypropylene, polyethylene, and polycarbonate sheets, and the thickness thereof is 30-200μm; The support body is made of PET material with a thickness of 100-200 um, including any one of polyethylene terephthalate, polybutylene terephthalate, and polyvinyl chloride.

3. An inorganic phosphorus dry detection and analysis component as claimed in claim 2, characterized in that: In the diffusion layer, the 5-sulfosalicylic acid is added in an amount of 1-20 g per unit square meter or 1-15 g per unit square meter; the succinic acid is added in an amount of 1-20 g per unit square meter or 1-7 g per unit square meter; the non-ionic polymer chemicals include any one or more of PVPK90, PVPK30, PVPK15, and PVPK17 and are added in an amount of 1-15 g per unit square meter or 1-10 g per unit square meter; the Triton X-100 is added in an amount of 1-10 g per unit square meter or 1-8 g per unit square meter.

4. An inorganic phosphorus dry detection and analysis component as claimed in claim 3, characterized in that: The modified 30% binder is added to the first reagent layer in an amount of 1-50 g per unit square meter or 1-40 g per unit square meter; The preparation method of the modified 30% binder is as follows: S1. Weigh acrylamide and N-vinyl pyrrolidone in a mass ratio of 8:2 and dissolve them in 100 g of ultrapure water. After dissolution, weigh 2% of N,N'-methylenebisacrylamide and 1% of N,N,N',N'-tetramethylethylenediamine and stir them evenly in the solution. S2, transferring the solution obtained in step S1 to a polymerization reactor, introducing nitrogen for 10 min, raising the temperature to 40° C. after the nitrogen is introduced, and maintaining the temperature for 30 min under the protection of nitrogen; S3. Add 0.4% potassium persulfate and sodium bisulfite, continue to react at a constant temperature for 10 minutes, then react for 4 hours under sealed conditions, take out, and test the viscosity with a viscometer. The binder that passes the test is the desired binder.

5. An inorganic phosphorus dry detection and analysis component as claimed in claim 4, characterized in that: The modified acrylic resin emulsion is added to the first reagent layer in an amount of 1-30g per unit square meter or 2-20g per unit square meter; the white glue is added to the first reagent layer in an amount of 0.05-30g per unit square meter or 0.1-20g per unit square meter; the ammonium molybdate is added to the first reagent layer in an amount of 0.05-3g per unit square meter or 0.1-2g per unit square meter; the ascorbic acid is added to the first reagent layer in an amount of 0.05-10g per unit square meter or 0.1-7g per unit square meter.

6. An inorganic phosphorus dry detection and analysis component as claimed in claim 5, characterized in that: The surfactant is added to the first reagent layer in an amount of 0.05-5 g per unit square meter or 0.07-2 g per unit square meter; The surfactant is a zwitterionic surfactant, a cationic surfactant or an anionic surfactant; the zwitterionic surfactant includes any one of Tween 20, Tween 80, Triton X-405 and Triton X-100; the cationic surfactant includes any one of hexadecyltrimethylammonium bromide and dodecyltrimethylammonium bromide; the anionic surfactant includes any one or more of sodium dodecylbenzene sulfonate and sodium dodecyl sulfate.

7. An inorganic phosphorus dry detection and analysis component as claimed in claim 6, characterized in that: The soluble cellulose ether is added to the first reagent layer in an amount of 1-30 g per unit square meter or 1-20 g per unit square meter; the soluble cellulose ether includes any one of methyl cellulose, hydroxyethyl methyl cellulose, carboxymethyl cellulose, ethyl cellulose, benzyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, cyanoethyl cellulose, benzyl cyanoethyl cellulose, carboxymethyl hydroxyethyl cellulose and phenyl cellulose.

8. An inorganic phosphorus dry detection and analysis component as claimed in claim 7, characterized in that: The defoamer is added to the first reagent layer in an amount of 0.1-5 ml per unit square meter or 0.5-2 ml per unit square meter; the defoamer includes any one or more of silicone defoamers, polyether defoamers, and composite defoamers.

9. An inorganic phosphorus dry detection and analysis component as claimed in claim 8, characterized in that: The active material is added to the first reagent layer in an amount of 1-50 g per unit square meter or 10-40 g per unit square meter; the active material includes any one or more of diatomaceous earth, volcanic ash, silica gel, fly ash, microcrystalline cellulose, bentonite, titanium dioxide, burnt clay, and cellulose powder.

10. An inorganic phosphorus dry detection and analysis component according to any one of claims 1 to 9, characterized in that: The preparation method of the assembly is as follows: A1. Solution preparation of the second reagent layer: weigh 30 g of D4 hydrogel and dissolve it in 100 ml of mixed alcohol, where the ratio of alcohol to water is 9:

1. Wait until it is completely dissolved to obtain the solution of the second reagent layer. A2. Solution preparation of the first reagent layer: weigh white glue, ammonium molybdate, and ascorbic acid respectively and add them to the aqueous solution in sequence. After they are completely dissolved, add active materials, modified acrylic resin emulsion, modified 30% binder, surfactant, and soluble cellulose ether to the solution in sequence. The stirring speed is controlled at 500 r / min and the stirring time is 2 h. A3. Continue to add defoamer to step A2. After adding, adjust the stirring speed from 500r / min to 400r / min. The stirring time is 1h. A4, use a heated doctor blade coater to coat the second reagent layer solution of 0.3g / ml D4 hydrogel on the PET substrate, the coating wet film thickness is 100um, after coating is completed, transfer to a 60°C oven for drying for 5 minutes, and after drying, transfer to a tray for standby use; A5, the first reagent layer slurry that has been stirred evenly in step A3 is moved onto the coated second reagent layer for a second coating, the coating wet film thickness is controlled to be 100-500 μm, and dried at a temperature range of 50°C-70°C, and the dry film thickness after drying is 50-200 μm; A6. Cut the first reagent layer, the second reagent layer and the diffusion layer gauze dried in step A5 into 9.5*9.5mm 2 The small pieces are fixed and assembled into inorganic phosphorus dry detection and analysis components using customized black PET plastic card sleeves as the upper and lower support layers.

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