Device and method for identifying types and concentrations of metabolites in sweat

Abstract

The invention discloses a device and a method for identifying types and concentrations of metabolites in sweat. Belongs to the technical field of ultrasound and the field of electrochemical sensing and comprises a base station, a piezoelectric plate driving power supply, a piezoelectric plate, an aluminum / copper electrode, a glass substrate, a vibrating rod and an electric signal detection unit, the specific method is as follows: electric signals collected by an ultrasonic enhanced sweat sensor are utilized, the electric signals generated by different metabolites and concentrations in sweat are extremely weak, and the sensibilization coefficients of ultrasonic to the different metabolites and concentrations in the sweat are different, so that the difference between the electric signals is amplified by the ultrasonic, and the sensitivity of the sweat is improved. And the sweat sensor can easily identify the component and the concentration of the detected substance in the sweat. The ultrasonic technology and the electrochemical sensing technology are combined, the sensitivity of the sweat sensor is enhanced, the detection time of the sensor is shortened, the accuracy of the sensor is improved, and the types and concentrations of different metabolites in sweat are identified through an ultrasonic method.

Description

Technical Field

[0001] The present invention belongs to the field of ultrasonic technology and electrochemical sensing, and relates to a device for identifying the types and concentrations of metabolites in sweat and an identification method thereof; specifically, it relates to a method for identifying the types and concentrations of different metabolites in sweat based on ultrasonic sensitization coefficients. Background Art

[0002] The concentrations of sugars, acids, chlorides, etc. in sweat can be used to detect physiological health conditions and diagnose and treat related diseases. Compared with conventional blood and urine tests, it has the advantages of non-invasiveness and real-time continuous detection. Due to the instability of sweat secretion and the low concentration of some components in the metabolites, it is impossible to accurately identify the types and concentrations of metabolites in sweat; when doing a lot of exercise, some components of the sweat produced will also change due to exercise, resulting in inaccurate identification results.

[0003] The identification of sweat requires the instantaneous detection of sweat, but the instability of sweat secretion and the difficulty of collection limit the instantaneous detection of sweat. As time goes by, the pH value of sweat and the activity of metabolites will also change, which will lead to inaccurate identification results. A sweat sensor described in the Chinese invention patent with publication number CN114569123A needs to collect sweat and store it until it is sufficient before testing, which cannot complete the identification of sweat components in a short time. Summary of the invention

[0004] In view of the above problems, the purpose of the present invention is to propose a device and method for identifying the types and concentrations of metabolites in sweat, which enhances the sensitivity of the sweat sensor while improving the accuracy of the sensor and provides an ultrasonic method for identifying components in sweat.

[0005] The technical solution of the present invention is: a device for identifying the type and concentration of metabolites in sweat according to the present invention comprises a base station, a piezoelectric film driving power supply, a piezoelectric film, an aluminum / copper electrode, a glass substrate, a vibrating rod and an electrical signal detection unit;

[0006] The base comprises two parts: an upper piezoelectric sheet support frame for supporting the piezoelectric sheet at the upper end and a base for supporting the glass substrate and fixing the aluminum / copper electrode at the lower end.

[0007] Furthermore, a glass substrate is arranged on the upper end of the base, and aluminum / copper electrodes are arranged on the glass substrate and the base.

[0008] Furthermore, one end of the semicircular aluminum / copper electrode is placed on the glass substrate, and the other end is fixedly placed on the base;

[0009] One end of the aluminum / copper electrode fixed on the base is connected with an electrical signal detection unit through a wire.

[0010] Furthermore, a piezoelectric sheet is arranged on the upper end of the upper piezoelectric sheet support frame.

[0011] Two ends of the piezoelectric sheet are connected with a piezoelectric sheet driving power source through wires.

[0012] Furthermore, the top end of the vibration rod passes through the upper piezoelectric sheet support frame and is placed on the vibration radiation surface of the piezoelectric sheet through epoxy resin, and the other end is placed in a chamber on the glass substrate that can store sweat and does not contact the glass substrate.

[0013] Furthermore, in the aluminum / copper electrode, the aluminum electrode and the copper electrode are of the same size and shape, and the diameter of the outer semicircle is 5mm-10mm.

[0014] Furthermore, the glass substrate is formed by stacking a round glass with a diameter of 18mm-20mm and a thickness of 1mm-1.5mm and a glass ring with the same outer diameter as the round glass, an inner diameter of 12mm-15mm and a thickness of 1mm-1.5mm.

[0015] Furthermore, the resonance frequency range of the piezoelectric piece is 20kHz-200kHz, the operating frequency f is between 103.93kHz-104.83kHz, the operating voltage is about 7.5V-7.7V, and the operating current is 135mA-150mA;

[0016] The diameter of the piezoelectric sheet is the same as that of the glass substrate, and is composed of a ceramic material with a thickness of 1.5 mm to 3 mm having an inverse piezoelectric effect and two copper sheets bonded together by epoxy resin, forming a sandwich structure.

[0017] Furthermore, the diameter of the vibrating rod 6 is between 1.5 mm and 3 mm, and the length is required to allow one end of the vibrating rod to be immersed in sweat without contacting the glass substrate, and the vibrating rod 6 is made of resin material.

[0018] Furthermore, the identification method of the device for identifying the type and concentration of metabolites in sweat has the following operating steps:

[0019] Step (1), placing sweat or a certain metabolite in sweat in a chamber capable of storing sweat on a glass substrate (5), the aluminum / copper electrode (4) being in full contact with the sweat or a certain metabolite in sweat, and the generated electrical signal being collected by an electrical signal detection unit (7);

[0020] Step (2), when the piezoelectric sheet driving unit (2) is turned off, the piezoelectric sheet (3) does not perform ultrasonic vibration, and the vibration rod (6) has no effect;

[0021] Step (3), when the piezoelectric sheet driving unit (2) is turned on, the piezoelectric sheet (3) is ultrasonically excited, and the vibration is transmitted to the vibration rod (6). The end of the vibration rod (6) away from the piezoelectric sheet is inserted into sweat or a metabolic product of sweat, and an acoustic flow field is generated therein; the electrical signal generated at this time is collected by the electrical signal detection unit (7);

[0022] Step (4), comparing the data collected by applying ultrasound with the data without applying ultrasound, the size of the electrical signal collected by applying ultrasound is in a certain ratio to the original size;

[0023] The current generated by chloride under ultrasound is about 6 times the original, urea and glucose can reach about 15 times the original, and acids can reach about 20 times the original;

[0024] Therefore, the electrical signals collected after ultrasound application of different types of metabolites and concentrations in sweat will fall within a specific range, thereby identifying the components and concentrations in the sweat.

[0025] The beneficial effects of the present invention are: 1. Ultrasound accelerates the transmission of ions in sweat and amplifies the electrical signals generated by sweat, making the electrical signals easier to be captured by the sweat detection unit, thereby improving the sensitivity of the sweat sensor; 2. Since ultrasound has different amplification effects on the electrical signals generated by different metabolites and concentrations in sweat, and the electrical signals generated by different metabolites and concentrations in sweat will fall within a specific interval of a certain range under the action of ultrasound, it is easy to identify which metabolite and concentration of the detected object is in sweat; 3. The piezoelectric buzzer that generates ultrasound is small in size and low in energy consumption, and can be attached to a flexible wearable intelligent detection device or a micro sensor. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a specific device structure diagram of the present invention;

[0027] Figure 2 It is a structural schematic diagram of the base platform in the present invention;

[0028] Figure 3 This is a graph of electrical signal acquisition data in an embodiment of the present invention (the sweat component is 1M NaCl);

[0029] In the figure, 1 is a base, 101 is a base, and 102 is an upper piezoelectric sheet support frame;

[0030] 2 is a piezoelectric film driving power source, 3 is a piezoelectric film, 4 is an aluminum / copper electrode, 5 is a glass substrate, 6 is a vibration rod, and 7 is an electrical signal detection unit. DETAILED DESCRIPTION

[0031] The specific technical scheme of the present invention is further described in detail below with reference to specific examples.

[0032] As shown in the figure, the device and method for identifying the type and concentration of metabolites in sweat of the present invention include a base 1, a piezoelectric film driving power supply 2, a piezoelectric film 3, an aluminum / copper electrode 4, a glass substrate 5, a vibrating rod 6 and an electrical signal detection unit 7;

[0033] The base 1 comprises an upper and a lower part. The base 101 at the lower end is used to support the glass substrate 5 and fix the aluminum / copper electrode 4 ; the piezoelectric sheet support frame 102 at the upper end is used to support the piezoelectric sheet 3 .

[0034] Furthermore, one end of the semicircular aluminum / copper electrode 4 is adhered to the glass substrate 5 with double-sided adhesive, and the other end is fixed to the base 101 in the same way;

[0035] The end of the aluminum / copper electrode 4 fixed on the base 101 is connected to an electrical signal detection unit 7 through a wire.

[0036] Furthermore, in the aluminum / copper electrode 4, the aluminum electrode and the copper electrode included therein are of the same size and shape, and the diameter of the semicircle at the outer end thereof is 5 mm-10 mm.

[0037] Furthermore, the glass substrate 5 is formed by stacking a round glass with a diameter of 18mm-20mm and a thickness of 1mm-1.5mm and a glass ring with the same outer diameter as the round glass, an inner diameter of 12mm-15mm and a thickness of 1mm-1.5mm.

[0038] Furthermore, the top end of the vibration rod 6 is adhered to the vibration radiation surface of the piezoelectric sheet 3 through epoxy resin, and the other end is placed in a chamber for storing sweat on the glass substrate 5 and does not contact the glass substrate 5.

[0039] Furthermore, a piezoelectric sheet 3 is mounted on the piezoelectric sheet support frame 102 at the upper end of the base 1.

[0040] The two ends of the piezoelectric sheet 3 are connected to a piezoelectric sheet driving power source 2 via wires.

[0041] Furthermore, the resonance frequency range of the piezoelectric sheet 3 is 20kHz-200kHz, the operating frequency f is between 103.93kHz-104.83kHz, the operating voltage is about 7.5V-7.7V, and the operating current is 135mA-150mA;

[0042] The piezoelectric sheet 3 has a diameter of 20 mm, which is the same as the diameter of the glass substrate 5 , and is composed of a ceramic material with a thickness of 1.5 mm to 3 mm having an inverse piezoelectric effect and two copper sheets bonded together by epoxy resin, forming a sandwich structure.

[0043] Furthermore, the diameter of the vibrating rod 6 is between 1.5 mm and 3 mm, and the length is required to allow one end of the vibrating rod to be immersed in sweat without contacting the glass substrate, and the vibrating rod 6 is made of resin material.

[0044] Furthermore, the method for identifying the type and concentration of metabolites in sweat comprises the following steps:

[0045] (1) placing sweat or a certain metabolite in sweat in a chamber on a glass substrate 5 capable of storing sweat, and the aluminum / copper electrode 4 is in full contact with the sweat or a certain metabolite in sweat, and the generated electrical signal is collected by an electrical signal detection unit 7;

[0046] (2) When the piezoelectric sheet 3 driving unit 2 is turned off, the piezoelectric sheet 3 does not perform ultrasonic vibration, and the vibrating rod 6 has no effect. The entire device is equivalent to a traditional sweat detection device;

[0047] (3) When the piezoelectric film driving unit 2 is turned on, the piezoelectric film 3 is ultrasonically excited, and the vibration is transmitted to the vibration rod 6. The end of the vibration rod 6 away from the piezoelectric film is inserted into sweat or a metabolic product of sweat, and an acoustic flow field is generated therein; the electrical signal generated at this time is collected by the electrical signal detection unit 7.

[0048] (4) Comparing the data collected with ultrasound and the data without ultrasound, the size of the electrical signal collected with ultrasound is in a certain ratio to the original size; the current generated by chloride under the action of ultrasound is about 6 times the original, urea and glucose can reach about 15 times the original, and acids can reach about 20 times the original; therefore, the electrical signals collected after the application of ultrasound for different types of metabolites and concentrations in sweat will fall within a certain range, so that the components and concentrations in the sweat can be clearly identified.

[0049] Furthermore, ultrasound has different sensitivity enhancement coefficients for different metabolite types and concentrations in sweat. By ultrasonically amplifying the electrical signals generated by different metabolites and concentrations in sweat, the sweat sensor can easily identify the component and concentration of the detected substance in sweat.

[0050] Furthermore, the device for implementing this method mainly includes a piezoelectric sheet 3 or other device capable of generating ultrasound, a microfluidic channel or method capable of collecting sweat, and a sweat detection unit for detecting electrical signals.

[0051] Furthermore, the electrical signals generated by different metabolites and concentrations in sweat can be amplified by various ultrasonic excitation methods and vibration modes.

[0052] Furthermore, the types of metabolites in sweat that can be identified by this method include but are not limited to chloride, acids, urea, glucose, etc.

[0053] Furthermore, the ultrasonic driving power source drives the piezoelectric sheet 3 to perform ultrasonic vibration, and the ultrasonic signal is transmitted to the vibration rod 6 connected to the piezoelectric sheet 3 by epoxy resin, and the other end of the vibration rod 6 is immersed in the sweat or a certain component of the sweat on the glass substrate 5, and an acoustic flow field is generated therein;

[0054] The aluminum electrode and the copper electrode are respectively attached to the two sides of the glass substrate 5 , and are partially immersed in sweat or some metabolites in the sweat, and the exposed parts are connected to the electrical signal detection unit 7 .

[0055] The acoustic flow field generated by the vibration rod 6 in the sweat or some of its components accelerates the transmission rate of ions, so ultrasound has an amplifying effect on the electrical signals generated by sweat or metabolic products in sweat; for substances such as NaCl and KCl that exist in the form of ions in the solution, the amplifying effect of ultrasound on the electrical signals generated by them is not obvious, only 4-6 times of their own electrical signals;

[0056] The electrical signals generated by substances such as urea and glucose are very weak, and they will deteriorate and hydrolyze over time, resulting in inaccurate test results. However, ultrasound can significantly amplify their electrical signals, which can reach about 15 times their own electrical signals.

[0057] The size of the electrical signal generated by acidic substances such as uric acid and lactic acid is between the above two types of substances, but the test results are inaccurate because the electrical signal generated by itself is weak. After ultrasonic amplification, it can reach about 20 times of its own electrical signal.

[0058] In summary, ultrasound has different sensitivity enhancement coefficients for different metabolites and concentrations, which can make the electrical signals it generates fall within a specific interval within a certain range, so that the detected substances can be identified and judged based on the size of their electrical signals.

[0059] In an embodiment,

[0060] The resonant frequency of the piezoelectric piece 3 is 104.5kHz, the operating frequency f=104.83kHz, the operating voltage is 7.7V (effective value), and the operating current is 150mA;

[0061] The driving power supply of the piezoelectric piece 3 is a signal generator that sends an electrical signal to a power amplifier for amplifying the power of the electrical signal, which is used to drive the piezoelectric piece 3, and an oscilloscope displays the working voltage value and working current value of the piezoelectric piece.

[0062] The glass substrate 5 is formed by stacking a round glass with a diameter of 20 mm and a thickness of 1 mm and a glass ring with an outer diameter of 20 mm, an inner diameter of 15 mm and a thickness of 1 mm, and is used to hold the object to be detected.

[0063] The diameter of the piezoelectric sheet 3 is 20 mm, which is the same as the diameter of the glass substrate 5 , and the thickness is 2 mm. Copper sheets with a thickness of 0.5 mm are attached to the upper and lower surfaces.

[0064] The aluminum electrode and the copper electrode are of the same size and shape. The diameter of the outer semicircle is 10 mm, the end connected to the electrical signal detection unit 7 is 50 mm long and 5 mm wide, and the overall thickness is 0.6 mm.

[0065] The electrical signal detection unit 7 is undertaken by the electrochemical workstation to collect and record the electrical signals generated by the metabolites.

[0066] The vibration rod 6 has a diameter of 2 mm and a length of 13.2 mm and is made of resin material.

[0067] The present invention proposes a method for identifying different types and concentrations of metabolites in sweat based on ultrasound sensitization coefficient. It is an ultrasound-based electrochemical identification method, which is achieved by utilizing the different amplification effects of ultrasound on electrical signals generated by different types and concentrations of metabolites in sweat.

Claims

1. A device for identifying the type and concentration of metabolites in sweat, characterized in that: It comprises a base (1), a piezoelectric sheet driving power supply (2), a piezoelectric sheet (3), an aluminum / copper electrode (4), a glass substrate (5), a vibrating rod (6) and an electrical signal detection unit (7); The base (1) comprises two parts: an upper piezoelectric sheet support frame (102) for supporting the piezoelectric sheet (3) and a lower base (101) for supporting the glass substrate (5) and fixing the aluminum / copper electrode (4).

2. The device for identifying the type and concentration of metabolites in sweat according to claim 1, characterized in that: A glass substrate (5) is arranged on the upper end of the base (101), and aluminum / copper electrodes (4) are arranged on the glass substrate (5) and the base (101).

3. The device for identifying the type and concentration of metabolites in sweat according to claim 2, characterized in that: One semicircular end of the aluminum / copper electrode (4) is placed on the glass substrate (5), and the other end is fixedly placed on the base (101); One end of the aluminum / copper electrode (4) fixed on the base (101) is connected to an electrical signal detection unit (7) via a wire.

4. The device for identifying the type and concentration of metabolites in sweat according to claim 1, characterized in that: A piezoelectric sheet (3) is arranged at the upper end of the upper piezoelectric sheet support frame (102). The two ends of the piezoelectric sheet (3) are connected to a piezoelectric sheet driving power source (2) via wires.

5. The device for identifying the type and concentration of metabolites in sweat according to claim 4, characterized in that: The top end of the vibrating rod (6) passes through the upper piezoelectric sheet support frame (102) and is placed on the vibration radiation surface of the piezoelectric sheet (3) through epoxy resin, and the other end is placed in a chamber on the glass substrate (5) that can store sweat and does not contact the glass substrate (5).

6. The device for identifying the type and concentration of metabolites in sweat according to claim 2, characterized in that: In the aluminum / copper electrode (4), the aluminum electrode and the copper electrode comprised therein are of the same size and shape, and the diameter of the semicircle at the outer end thereof is 5 mm to 10 mm.

7. The device for identifying the type and concentration of metabolites in sweat according to claim 2, characterized in that: The glass substrate (5) is formed by stacking a round glass with a diameter of 18 mm to 20 mm and a thickness of 1 mm to 1.5 mm and a glass ring with the same outer diameter as the round glass, an inner diameter of 12 mm to 15 mm and a thickness of 1 mm to 1.5 mm.

8. The device for identifying the type and concentration of metabolites in sweat according to claim 4, characterized in that: The resonant frequency range of the piezoelectric sheet (3) is 20kHz-200kHz, the operating frequency f is between 103.93kHz-104.83kHz, the operating voltage is about 7.5V-7.7V, and the operating current is 135mA-150mA; The diameter of the piezoelectric sheet (3) is the same as that of the glass substrate (5), and is composed of a ceramic material with a reverse piezoelectric effect having a thickness ranging from 1.5 mm to 3 mm and two copper sheets bonded together by epoxy resin, forming a sandwich structure.

9. The device for identifying the type and concentration of metabolites in sweat according to claim 5, characterized in that: The diameter of the vibration rod (6) is between 1.5 mm and 3 mm and is made of resin material.

10. The identification method of the device for identifying the type and concentration of metabolites in sweat according to any one of claims 1 to 9, characterized in that: The operation steps are as follows: Step (1), placing sweat or a certain metabolite in sweat in a chamber capable of storing sweat on a glass substrate (5), the aluminum / copper electrode (4) being in full contact with the sweat or a certain metabolite in sweat, and the generated electrical signal being collected by an electrical signal detection unit (7); Step (2), when the piezoelectric sheet driving unit (2) is turned off, the piezoelectric sheet (3) does not perform ultrasonic vibration, and the vibration rod (6) has no effect; Step (3), when the piezoelectric sheet (3) driving unit (2) is turned on, the piezoelectric sheet (3) is ultrasonically excited, and the vibration is transmitted to the vibrating rod (6). The end of the vibrating rod (6) away from the piezoelectric sheet is inserted into sweat or a metabolic product of sweat, and an acoustic flow field is generated therein; the electrical signal generated at this time is collected by the electrical signal detection unit (7); Step (4), comparing the data collected by applying ultrasound with the data without applying ultrasound, the size of the electrical signal collected by applying ultrasound is in a certain ratio to the original size; The current generated by chloride under ultrasound is about 6 times the original, urea and glucose can reach about 15 times the original, and acids can reach about 20 times the original; Therefore, the electrical signals collected after ultrasound application of different types of metabolites and concentrations in sweat will fall within a specific range, thereby identifying the components and concentrations in the sweat.

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