Agricultural composition for controlling vigorous growth of plants

Abstract

The invention relates to the technical field of agricultural compositions, in particular to an agricultural composition for controlling vigorous growth of plants. The active ingredients of the agricultural composition for controlling vigorous growth of plants are composed of furfuryl aminopurine and prohexadione calcium, the prohexadione calcium and furfuryl aminopurine are mixed according to the mass ratio of (0.1-90): (0.01-50), the vigorous growth of plants can be improved and controlled, the vigorous growth control effect is better than the effect of each single agent through compounding of furfuryl aminopurine and prohexadione calcium, and the synergistic effect is obvious; in addition, a field test result shows that the yield is increased when the mixing mass ratio of furfurylaminopurine to prohexadione calcium is (0.1-90): (0.01-50), and the agricultural composition can be used for controlling vigorous growth and increasing yield of plants and has a good application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural compositions, and in particular to an agricultural composition for promoting fruit enlargement. Background Art

[0002] Plant growth control is an important link in agricultural production and an important technical measure to regulate plant growth rate and improve yield and quality. Currently, some growth control agents have been reported, but they are far from enough. More growth control products or growth control compositions need to be developed.

[0003] Prohexadione calcium, chemical name is calcium 3,5-dioxo-4-propionylcyclohexanecarboxylate, a plant growth regulator, which has the effects of inhibiting plant growth, making the plant root system well-developed, the stems thick, the internodes shortened, and enhancing lodging resistance; increasing chlorophyll content, enhancing photosynthesis; promoting flower bud differentiation, increasing fruit setting rate, promoting fruit enlargement, promoting root and tuber enlargement, regulating plant endogenous hormones, and enhancing stress resistance and disease resistance. Prohexadione calcium can be quickly absorbed by crop leaf cells and directly act on the target.

[0004] Furfurylaminopurine, also known as kinetin or 6-furfurylaminopurine, is the first cytokinin discovered by humans. It is a substance that can promote plant cell division and differentiation. It is widely present in seaweed and most plants. It is an endogenous (natural) plant growth regulator produced by plants that has a regulatory effect on plant growth. Its functions mainly include promoting cell division, differentiation, and growth; inducing callus sprouts and relieving apical dominance; breaking the dormancy of lateral buds and promoting seed germination; delaying the senescence of detached leaves and cut flowers; inducing bud differentiation and development and increasing stomatal aperture; regulating the transport of nutrients; and promoting fruiting.

[0005] CN103518737A discloses an active ingredient including: prohexadione calcium, paclobutrazol and a plant growth promoter; used for controlling the vigorous growth of crops such as peanuts, soybeans or rapeseed. Mixing existing ingredients is an effective technical means to find new vigorous growth promoters. However, ordinary technicians in this field cannot predict whether prohexadione calcium and furfural aminopurine can be mixed without a large number of experimental verifications, and whether the mixing of the two can achieve synergistic or antagonistic effects. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides an agricultural composition for controlling plant growth, aiming to find a substance that can be mixed with prohexadione-calcium for controlling plant growth and increasing yield.

[0007] On the one hand, the present invention provides an agricultural composition for controlling plant growth, wherein the active ingredients of the agricultural composition for controlling plant growth are composed of prohexadione-calcium and furfural aminopurine, and the mass ratio of prohexadione-calcium to furfural aminopurine is 0.1-90:0.01-50.

[0008] Furthermore, in the agricultural composition for controlling plant growth, the mass ratio of prohexadione-calcium to furfural aminopurine is 30-60:0.1-0.5, based on the mass ratio.

[0009] Furthermore, in the agricultural composition for controlling plant growth, the mass ratio of prohexadione-calcium to furfural aminopurine is 50:0.25.

[0010] In another aspect, the present invention provides the use of an agricultural composition for controlling plant growth to prepare a plant growth controlling agent.

[0011] Furthermore, the plant growth control agent of the plant growth control agricultural composition is applied to the leaves of plants.

[0012] Furthermore, the plant growth control agent is applied to apple leaves, peanut leaves, and strawberry leaves.

[0013] In addition, the present invention also provides application of the agricultural composition for controlling plant growth in increasing fruit yield.

[0014] Furthermore, the agricultural composition for controlling plant growth is used to increase the weight of individual apple fruits.

[0015] Furthermore, the agricultural composition for controlling plant growth is used to increase strawberry yield.

[0016] Furthermore, the agricultural composition for controlling plant growth is used to increase peanut yield.

[0017] It is easy for those skilled in the art to know that in order to give full play to the efficacy of the active components of the agricultural composition, it can be prepared into a formulation that is easy to apply according to the needs of use. Based on this, further, the present invention provides an agricultural composition for controlling plant vigor. The agricultural composition is processed into an agriculturally acceptable formulation, and the formulation contains an auxiliary agent that assists the furfural aminopurine and prohexadione calcium in improving the plant vigor control performance and an auxiliary agent that increases fruit yield. The agricultural composition can be formulated into a variety of formulations such as soluble liquids, water-dispersible granules, wettable powders, dispersible oil suspensions, suspensions, microemulsions, granules, and microcapsules with a certain sustained release effect. When preparing the above-mentioned different formulations, for those skilled in the art, in addition to using the agricultural composition containing the optional active ingredients for controlling plant vigor, it is also necessary to select a variety of auxiliary agents, and different formulation auxiliary ingredients (auxiliaries) can be selected and used as needed. The auxiliary component may be one or more of a dispersion medium, a dispersant, an emulsifier, a wetting agent, a thickener, a defoaming agent, an antifreeze agent, a disintegrant, a binder, a filler, a carrier, and the like.

[0018] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0019] (1) The contribution of the present invention to the prior art is to provide an agricultural composition for controlling plant vigor, wherein the active ingredients are composed of furfurylaminopurine and prohexadione calcium, and furfurylaminopurine and prohexadione calcium have a vigor-control effect on plant growth within a certain ratio range, and after furfurylaminopurine and prohexadione calcium are mixed, the control of plant vigor is better than that of a single agent, and the synergistic effect is obvious. In particular, when the mass ratio of furfurylaminopurine to prohexadione calcium is 50:0.25, the synergistic effect reaches the highest.

[0020] (2) The agricultural composition for controlling plant growth containing furfural aminopurine and prohexadione calcium provided by the present invention has field test results showing that when the mass ratio of furfural aminopurine to prohexadione calcium is within the range of 0.1-90:0.01-50, the fruit yield is increased. When the mass ratio of furfural aminopurine to prohexadione calcium is 50:0.25, the yield increase rate of strawberries reaches 17.59%. DETAILED DESCRIPTION

[0021] The technical scheme of the present invention is described below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0022] Example 1

[0023] This example is a field test experiment on the effect of a mixture of prohexadione-calcium and furfural aminopurine on controlling the growth of peanut plants and peanut yield.

[0024] The peanut variety used for the test was Huayu No. 22, provided by Shandong Peanut Research Institute.

[0025] Treatment of test agents: The test agents calcium prohexadione and furfural aminopurine were first dissolved in methanol and then diluted with 0.1% Tween-80 aqueous solution to prepare single-dose stock solutions and mixed stock solutions of various proportions. Different mass concentration gradients were set for the single doses and the mixed formulations for later use.

[0026] Dilute the above-mentioned mixtures of different concentrations and single-dose stock solutions with clean water at a ratio of 1:1000 and set aside.

[0027] The test set up a water seed soaking group (KC group), a single agent group for testing, and a mixed agent group, i.e., a growth-controlling agricultural composition test group.

[0028] In the peanut field, peanut plants with the same growth were selected. At the initial flowering stage of peanuts, the diluted mixture of prohexadione calcium and furfural aminopurine and the single agent were sprayed evenly on the leaves of peanuts, and the group sprayed with water at the same time served as the blank control group. The trait indexes and yield composition of peanut plants were measured at the peanut harvest stage.

[0029] For example, in test group 1, 0.1 g of prohexadione calcium and 60 g of furfural aminopurine were first dissolved in methanol, and then diluted with 0.1% Tween-80 aqueous solution to a total mass of 100 g, that is, a plant vigor-controlling agricultural composition with a mass ratio of prohexadione calcium: furfural aminopurine = 0.1:60 was prepared, and the plant vigor-controlling agricultural composition of test group 1 was diluted with clean water at 1:1000, and evenly sprayed on the leaves during the early flowering period of peanuts. The trait indicators and yield composition of peanut plants were measured at the peanut harvest period.

[0030] The Gowing method was used to evaluate the effects of the effective components on the growth control performance of peanuts after mixing, and the formula was E0=X+[Y×(100-X) / 100], where E0 represented the theoretical main stem growth control rate, side stem growth control rate, and yield increase rate of the peanuts of the combination (A+B) when the dosage was (P+Q), X represented the main stem growth control rate, side stem growth control rate, and yield increase rate of peanuts treated with agent A when the dosage was P, and Y represented the main stem growth control rate, side stem growth control rate, and yield increase rate of peanuts treated with agent B when the dosage was Q; and E represented the main stem growth control rate, side stem growth control rate, and yield increase rate of the actual peanuts treated with the combination.

[0031] When E-E0>10%, it is a synergistic effect; when E-E0<-10%, it is an antagonistic effect; when the E-E0 value is between ±10%, it is an additive effect.

[0032] Main stem vigor control rate (%) = (main stem height of experimental group - main stem height of blank control group) / main stem height of blank control group × 100

[0033] Lateral stem vigor control rate (%) = (lateral stem length of experimental group - lateral stem length of blank control group) / lateral stem length of blank control group × 100

[0034] Yield increase rate (%) = (yield of test group - yield of blank control group) / yield of blank control group × 100

[0035] The test results of the effects of different treatments on peanut growth control are shown in Table 1; the test results of the effects of different treatments on peanut yield are shown in Table 2.

[0036] Table 1 Test results of the effects of different treatments on peanut vigor control

[0037]

[0038]

[0039] The results in Table 1 show that when the mass ratio of prohexadione calcium to furfural aminopurine is in the range of 0.1-90:0.01-50, a synergistic effect is exhibited on controlling the growth of peanut plants; in particular, when the mass ratio of prohexadione calcium to furfural aminopurine is in the range of 30-60:0.1-0.5, a significant synergistic effect is exhibited on controlling the growth of peanut plants; when the mass ratio of prohexadione calcium to furfural aminopurine is 50:0.25, the synergistic effect on controlling the growth of peanut plants is optimal.

[0040] Table 2 Test results of the effects of different treatments on peanut yield increase

[0041]

[0042]

[0043]

[0044] The results in Table 2 show that when the mass ratio of calcium hexadione to furfural aminopurine is in the range of 0.1-90:0.01-50, a synergistic effect is shown in promoting peanut yield increase; especially when the mass ratio of calcium hexadione to furfural aminopurine is in the range of 30-60:0.1-0.5, the synergistic effect on promoting peanut yield increase is significant; when the mass ratio of calcium hexadione to furfural aminopurine is 50:0.25, the synergistic effect on promoting peanut yield increase is the best.

[0045] Example 2

[0046] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0047] Weigh 100 g of prohexadione calcium and 5000 g of furfural aminopurine, dissolve them in methanol, and then dilute them with a 0.1% Tween-80 aqueous solution until the total mass is 10000 g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfural aminopurine = 1:50. Set it as 1# agricultural composition and set aside.

[0048] Example 3

[0049] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0050] Weigh 200g of prohexadione calcium and 500g of furfurylaminopurine, dissolve them in methanol, and then dilute them with 0.1% Tween-80 aqueous solution until the total mass is 10000g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfurylaminopurine = 2:5. Set it as 2# agricultural composition and set aside.

[0051] Example 4

[0052] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0053] Weigh 1000g of prohexadione calcium and 300g of furfurylaminopurine, dissolve them in methanol, and then dilute them with a 0.1% Tween-80 aqueous solution until the total mass is 10000g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfurylaminopurine = 10:3. Set it as 3# agricultural composition and set aside.

[0054] Example 5

[0055] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0056] Weigh 2000g of prohexadione calcium and 100g of furfurylaminopurine, dissolve them in methanol, and then dilute them with a 0.1% Tween-80 aqueous solution until the total mass is 10000g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfurylaminopurine = 20:1. Set it as 4# agricultural composition and set aside.

[0057] Example 6

[0058] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0059] 3000 g of prohexadione calcium and 50 g of furfural aminopurine were weighed, dissolved in methanol, and then diluted with a 0.1% Tween-80 aqueous solution until the total mass was 10000 g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfural aminopurine = 30:0.5. This was set as 5# agricultural composition and set aside.

[0060] Example 7

[0061] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0062] 5000 g of prohexadione calcium and 25 g of furfural aminopurine were weighed, dissolved in methanol, and then diluted with a 0.1% Tween-80 aqueous solution until the total mass was 10000 g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfural aminopurine = 50:0.25. This was set as 6# agricultural composition and set aside.

[0063] Example 8

[0064] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0065] 6000 g of prohexadione calcium and 10 g of furfural aminopurine were weighed, dissolved in methanol, and then diluted with a 0.1% Tween-80 aqueous solution until the total mass was 10000 g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfural aminopurine = 60:0.1. This was set as 7# agricultural composition and set aside.

[0066] Example 9

[0067] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0068] 8000 g of prohexadione calcium and 5 g of furfural aminopurine were weighed, dissolved in methanol, and then diluted with a 0.1% Tween-80 aqueous solution until the total mass was 10000 g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfural aminopurine = 80:0.05. This was designated as agricultural composition #8 and set aside.

[0069] Example 10

[0070] This embodiment is a plant growth control agricultural composition prepared by mixing prohexadione calcium and furfural aminopurine.

[0071] 9000 g of prohexadione calcium and 1 g of furfural aminopurine were weighed, dissolved in methanol, and then diluted with a 0.1% Tween-80 aqueous solution until the total mass was 10000 g, thus preparing a plant growth control agricultural composition with a mass ratio of prohexadione calcium to furfural aminopurine = 90:0.01. This was set as 9# agricultural composition and set aside.

[0072] Embodiment 11

[0073] This example is a field test experiment on the effect of a mixture of prohexadione-calcium and furfural aminopurine on controlling apple growth and single apple weight.

[0074] Apple varieties for testing: 5-year-old Red Fuji apples from Weinan City, Shaanxi Province were selected as the test varieties.

[0075] The experiment was conducted in the apple-growing area of ​​Weinan City, Shaanxi Province, and the spray method was used for pesticide application from late May to mid-June 2023.

[0076] The experiment set up a test group (1#-9# agricultural composition group); a control group of 0.4% furfural aminopurine (KC1) and 4.9% prohexadione calcium (KC2); and a blank control group of clear water (CK).

[0077] During the vigorous growth period of new apple shoots, dilute the agricultural compositions of 1#-9# and KC1 and KC2 with clean water at a ratio of 1:1000, and spray the dilutions of the above groups and the clean water of the clean water group evenly on the apple leaves. Spray the whole plant, spray each plant twice, until the leaves drip, and each time is 10 days apart. Choose the morning or evening when there is no wind and it is cloudy, avoid the high temperature period, and other field operations are handled according to the routine.

[0078] Each plant was a plot, and each treatment was repeated 3 times in random blocks. The branch growth and apple single fruit situation of each group were investigated. The results are shown in Table 3.

[0079] Table 3 Effects of different treatments on apple branch growth and single apple fruit weight

[0080] Trial Groups Branch growth length (cm) Branch growth thickness (cm) Apple weight (g) Experimental Group 1 5.68 0.09 252.91 Experimental Group 2 4.71 0.11 256.76 Experimental Group 3 4.18 0.12 261.05 Experimental Group 4 2.75 0.15 265.17 Experimental Group 5 2.71 0.18 277.36 Experimental Group 6 2.02 0.21 290.32 Experimental Group 7 3.98 0.19 278.11 Experimental Group 8 4.27 0.09 255.95 Experimental Group 9 5.31 0.08 251.32 KC1 9.76 0.04 239.01 KC2 8.56 0.07 241.15 CK 9.75 0.03 204.12

[0081] The effects of different treatments on apple branch growth and apple fruit weight are shown in Table 3. When the mass ratio of prohexadione calcium to furfural aminopurine is in the range of 0.1-90:0.01-50, the growth of apple branches can be controlled; especially when the mass ratio of prohexadione calcium to furfural aminopurine is in the range of 30-60:0.1-0.5, the growth control effect on apple branches is significant; especially when the mass ratio of prohexadione calcium to furfural aminopurine is 50:0.25, the growth control effect on apple branches is significant. The control effect on branch growth is the best; when the mass ratio of calcium oxadiazine to furfural aminopurine is in the range of 0.1-90:0.01-50, the single fruit weight of apples increases compared with the control; especially when the mass ratio of calcium oxadiazine to furfural aminopurine is in the range of 30-60:0.1-0.5, the single fruit weight of apples increases significantly compared with the control; especially when the mass ratio of calcium oxadiazine to furfural aminopurine is 50:0.25, the single fruit weight of apples increases the most compared with the control.

[0082] Example 12

[0083] This example is a field test experiment on the effect of a mixture of prohexadione-calcium and furfural aminopurine on controlling the growth of strawberry plants and strawberry yield.

[0084] The strawberry variety tested: Hongyan.

[0085] The trial was conducted in a nursery in Xi'an, Shaanxi Province, and the pesticide was applied by spraying in June 2023.

[0086] The experiment set up a test group (1#-9# agricultural composition group); a control group of 0.4% furfural aminopurine (KC1) and 4.9% prohexadione calcium (KC2); and a blank control group of clear water (CK).

[0087] The test variety for the field control experiment of strawberry seedling stage is Hongyan, and the water and fertilizer management and pest and disease control follow the conventional treatment. In the nursery period, the agricultural composition of 1#-9# and KC1 and KC2 were diluted with clean water at 1:1000, and the dilutions of the above groups and the clean water of the clean water group were evenly sprayed on the strawberry leaves. The whole plant was sprayed, and each plant was sprayed once until the leaves dripped. The spraying was selected in the morning or evening when there was no wind and it was cloudy, avoiding the high temperature period. The main stem height, branch and leaf deformity rate, per mu yield and yield increase rate of each group were investigated respectively. The results are shown in Table 4.

[0088] Yield increase rate (%) = (yield per mu of experimental group - yield per mu of blank control group) / yield per mu of blank control group × 100 Table 4 Effects of different treatments on strawberry vigor control and yield increase

[0089]

[0090]

[0091] The effects of different treatments on strawberry vigor control and yield increase are shown in Table 4. When the mass ratio of prohexadione calcium to furfural aminopurine is in the range of 0.1-90:0.01-50, strawberry vigor control is achieved; especially when the mass ratio of prohexadione calcium to furfural aminopurine is in the range of 30-60:0.1-0.5, strawberry vigor control is significantly effective; especially when the mass ratio of prohexadione calcium to furfural aminopurine is 50:0.25, strawberry vigor control is best. When the mass ratio of calcium prohexadione to furfural aminopurine is within the range of 0.1-90:0.01-50, the yield increase rate of strawberry is greater than 8% compared with the control; especially when the mass ratio of calcium prohexadione to furfural aminopurine is within the range of 30-60:0.1-0.5, the yield increase rate of strawberry is greater than 13% compared with the control; especially when the mass ratio of calcium prohexadione to furfural aminopurine is 50:0.25, the yield increase rate of strawberry is as high as 17.59% compared with the control.

[0092] The embodiments described above are part of the embodiments of the present invention, but not all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. An agricultural composition for controlling plant growth, characterized in that: The active ingredients are composed of prohexadione calcium and furfurylaminopurine, and the mass ratio of prohexadione calcium to furfurylaminopurine is 0.1-90:0.01-50.

2. The agricultural composition for controlling plant growth according to claim 1, characterized in that: In terms of mass ratio, the mass ratio of prohexadione calcium to furfural aminopurine is 30-60:0.1-0.

5.

3. The agricultural composition for controlling plant growth according to claim 2, characterized in that: In terms of mass ratio, the mass ratio of prohexadione calcium to furfural aminopurine is 50:0.

25.

4. The agricultural composition for controlling plant growth as claimed in claims 1 to 3 is used in the preparation of a plant growth controlling agent.

5. The plant growth control agent according to claim 4, characterized in that: The growth control agent is applied to the leaves of plants.

6. The plant growth control agent according to claim 5, characterized in that: The plant growth control agent is applied to the leaves of apple plants, peanut plants and strawberry plants.

7. Use of the agricultural composition for controlling plant growth as claimed in claims 1 to 3 in increasing fruit yield.

8. The use according to claim 7, characterized in that: The agricultural composition is used for increasing the weight of single apple.

9. The use according to claim 7, characterized in that: The agricultural composition is used for increasing the yield of strawberries.

10. The use according to claim 7, characterized in that: The agricultural composition is used for increasing the yield of peanuts.

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