Formula and preparation method of compound micro-ecological complete feed for weaned rabbits

Abstract

The invention belongs to the technical field of feed additives, and particularly relates to a weaning young rabbit compound micro-ecological complete feed formula and a preparation method, and the weaning young rabbit compound micro-ecological complete feed formula comprises the following components: 15.272 parts of wheat flour, 5 parts of glucose, 0.35 part of vitamin and mineral substance, 14.78 parts of soybean meal, 16 parts of rice bran, 45.56 parts of alfalfa, 0.01 part of vitamin C, 0.1 part of calcium bicarbonate, 0.708 part of bentonite, 0.35 part of calcium carbonate, 0.25 part of sodium chloride, 1.43 parts of soybean oil, 0.14 part of methionine, 0.05 part of choline chloride, and a compound micro-ecological additive; the compound micro-ecological additive is prepared from 108 to 1013cfu of rabbit source lactobacillus plantarum, 108 to 1013cfu of lanthanum-containing rabbit source lactobacillus plantarum and 100mg of vitamin C in terms of each kilogram of complete feed. The traditional Chinese medicine composition can regulate the form of intestinal tracts, solve the problem of diarrhea and improve the health of the intestinal tracts.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and in particular relates to a formula and a preparation method of a composite microecological complete feed for weaned rabbits. Background Art

[0002] Rabbits are important meat rabbits, and diarrhea has been a problem that has plagued the rabbit industry, causing great economic losses and is the most difficult problem in rabbit production and rabbit health care. The incidence of diarrhea is highest in rabbits within 3 weeks of weaning, which greatly affects the survival rate.

[0003] There are many factors that can cause diarrhea, including various environmental stresses, environmental factors, parasites, viruses, bacteria, feed factors, etc. After weaning, the young rabbits are prone to intestinal indigestion and poor absorption capacity, resulting in diarrhea, abdominal distension, constipation and other digestive system symptoms. The physiological characteristics of young rabbits are that the digestive organ system needs a gradual improvement process, the digestive physiological function is not sound, and the intake of ingredients and the absorption objects of feed nutrients after weaning undergo a major change, which requires a running-in period for the digestive physiological change. During this period, if their physiological characteristics are not taken into consideration, and if the feed is suddenly changed, the feeding amount is increased, the feed formula is unreasonable, the feed mycotoxins exceed the standard, and other pathogenic factors (such as high humidity, pathogen invasion, etc.), and the pursuit of high-speed growth is pursued, the possibility of diarrhea will increase.

[0004] Therefore, scientific feed formulation, reasonable feeding and management are necessary to reduce the problems of diarrhea causing death, weak rabbits or slow growth in weaned rabbits.

[0005] Currently, antibiotics, chlorpheniramine hydrochloride or diclazuril are regularly added to feed and drinking water to prevent the occurrence of post-weaning enteritis, and probiotics and digestive enzymes are added to prevent diarrhea and improve the intestinal environment. However, antibiotics and diclazuril can cause drug resistance, chlorpheniramine hydrochloride can affect meat quality, probiotics are mostly heterologous probiotics, and the preventive effect is weakened. Digestive enzymes can only simply enhance the digestion of certain nutrients but cannot guarantee normal absorption.

[0006] In addition, when the gastrointestinal function is normal, the beneficial and harmful microorganisms in the intestine are in a state of mutual restraint, maintaining a balance and preventing the rabbits from getting sick. However, after weaning, the rabbits will experience stress reactions, suppressed immune functions, reduced digestive enzyme activity, etc., which will damage the structure and function of the digestive organs and reduce their resistance. The pathogenic E. coli in the intestines will take the opportunity to multiply and produce toxicity, leading to diarrhea. At this time, the intestinal energy, vitamins and minerals are lost, and timely supply of glucose, vitamins and minerals is needed.

[0007] Studies have shown that lactic acid bacteria have an antagonistic effect on pathogenic bacteria in the intestines. Aerobic bacteria colonizing in the intestines of humans and animals are potential pathogens, and anaerobic bacteria play an important role in controlling potential pathogens. Lactic acid bacteria are important anaerobic bacteria in the intestines of animals. They can colonize normally in the intestines of animals, produce acidic substances such as lactic acid through metabolism, lower the pH value of the intestine, reduce the redox potential, produce hydrogen peroxide and other antibacterial substances such as bacteriocins to stabilize the normal flora, and compete with pathogenic bacteria for nutrients and attachment sites, thereby reducing the infection ability of pathogenic bacteria.

[0008] At present, many probiotics for livestock and poultry are mostly heterologous probiotics, and the types of bacteria are mostly single bacteria. Studies have shown that homologous lactic acid bacteria are more advantageous than heterologous lactic acid bacteria. There are many types of lactic acid bacteria, and lactic acid bacteria generally used to regulate intestinal flora include plant lactobacillus, but there is no rabbit homologous plant lactobacillus product. At present, rabbit plant lactobacillus has not been applied to the complete feed of weaned rabbits.

[0009] The specification of Chinese patent CN 112358981 records a plant lactobacillus and its application in weaned rabbit feed. The plant lactobacillus PLBS007 has good thermal stability and acid stability. Adding an appropriate level of plant lactobacillus PLBS007 to the diet of weaned rabbits can reduce the diarrhea rate and mortality rate of weaned rabbits, increase the average daily weight gain and immunoglobulin content of weaned rabbits, enhance the immune ability of weaned rabbits, reduce weaning stress, and improve the economic benefits of weaned rabbits. However, the plant lactobacillus in this patent is derived from cow's milk. According to existing reports, the effect of heterologous probiotics is poor compared with homologous probiotics. Summary of the invention

[0010] In order to solve the above technical problems, the present invention provides a formula of a composite microecological complete feed for weaned rabbits, a preparation method and an application thereof. Rabbit intestinal Lactobacillus plantarum is extracted from fresh chyme in the rabbit intestine for the first time. The complete feed microecological additive is a new type of lanthanum-containing Lactobacillus plantarum, which is combined with wheat flour, glucose, vitamins, minerals, soybean meal, rice bran, alfalfa, vitamin C and the like, so as to regulate the intestinal morphology, alleviate the problem of intestinal diarrhea and improve intestinal health. The complete feed is non-toxic, residue-free, does not produce drug resistance, has good safety, has good adhesion in the intestines of livestock and poultry, and has a high colonization survival rate of Lactobacillus plantarum. The preparation method is simple and the cost is low.

[0011] The present invention solves the above technical problems. A weaned rabbit composite microecological complete feed formula comprises the following ingredients (%): wheat flour 15.272, glucose 5, vitamins and minerals 0.35, soybean meal 14.78, rice bran 16, alfalfa 45.56, vitamin C 0.01, calcium bicarbonate 0.1, bentonite 0.708, calcium carbonate 0.35, sodium chloride 0.25, soybean oil 1.43, methionine 0.14, choline chloride 0.05, composite microbial additives; wherein, per kilogram of complete feed, the composite microbial additive comprises 10 lanthanum-containing rabbit-derived Lactobacillus plantarum 8 -10 13 cfu / kg, Lactobacillus plantarum from rabbit 10 8 -10 13 cfu / kg and vitamin C 100mg.

[0012] Feed raw materials are an important source of exogenous nutrients for weaned rabbits, and energy feed accounts for a large proportion. Therefore, the present invention uses food-grade energy feed raw materials, namely wheat flour, and does not use feed raw materials with a high risk of mildew, such as corn.

[0013] The lanthanum-containing rabbit-derived Lactobacillus plantarum can promote the fermentation of lactobacillus, accelerate the growth rate of lactobacillus, and reduce production costs.

[0014] The rabbit-derived plant lactobacillus in the present invention is a homologous probiotic with better effect. Vitamin C, as an anti-stress substance, can relieve weaning stress.

[0015] The composite rabbit-derived Lactobacillus plantarum and the lanthanum-containing rabbit-derived Lactobacillus plantarum are novel endogenous lactobacilli in rabbit intestines, and are extracted from fresh chyme collected from rabbit intestines within 30 minutes.

[0016] The culture medium used in the rabbit-source plant lactobacillus extraction process is MRS solid culture medium and lactobacillus selective culture medium.

[0017] In g / L, the lactobacillus selective culture medium has the following components:

[0018] Peptone 10, bean extract powder 10, yeast extract powder 2, sodium acetate 5, triammonium citrate 2, magnesium sulfate 0.2, manganese sulfate 0.05, Tween 80, glucose 20, calcium carbonate 5, agar 25, pH value 6-6.5.

[0019] The novel lanthanum-containing rabbit-derived Lactobacillus plantarum is composed of the following components (g / L):

[0020] Peptone 10, beef powder 8, yeast powder 4, glucose 20, dipotassium hydrogen phosphate 2, diammonium hydrogen citrate 2, sodium acetate 5, magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1, pH value 5.5-5.9, lanthanum chloride heptahydrate, 0.13-0.15.

[0021] The vitamins and minerals contained in each kilogram are as follows: Vitamin A, 10,000 IU; Vitamin D3, 1,200 IU; DL-α-tocopherol, 40 mg; Vitamin K3, 2 mg; Biotin, 0.4 mg; Niacin, 50 mg; Vitamin B7, 4 mg; Thiamine, 4 mg; Riboflavin, 6 mg; Cobalamin, 0.2 mg; Calcium pantothenate, 20 mg; Folic acid, 5 mg; Fe (FeSO4·H2O), 50 mg; Cu (CuSO4·5H2O), 10 mg; Mn (MnSO4·H2O), 8.5 mg; Co (CoSO4·7H2O), 0.25 mg; I (KI), 0.2 mg; Zn (ZnSO4·H2O), 50 mg; Se (Na2SeO3), 0.05 mg.

[0022] The present invention provides a method for preparing a composite microecological complete feed formula for weaned rabbits, comprising the following steps:

[0023] (1) preparing rabbit-derived Lactobacillus plantarum, lanthanum-containing rabbit-derived Lactobacillus plantarum and vitamin C into coated freeze-dried powders and packaging them into bags;

[0024] (2) Weighing and mixing other raw materials evenly and packaging them into individual bags;

[0025] (3) When feeding, add the freeze-dried powder to the other raw materials in step (2) under the conditions of an ambient temperature of 15-25° C. and an ambient humidity of 50±3% and mix well.

[0026] The number of viable bacteria per gram of freeze-dried powder of each bacterium in step (1) is 10 10 -10 11 cfu / g. The viable count refers to the number of viable bacteria per gram of the produced bacterial powder product.

[0027] The rabbit-derived plant lactobacillus is a novel endogenous lactic acid bacteria in the intestinal tract of rabbits. It is extracted from fresh chyme collected from the intestinal tract of healthy rabbits within 30 minutes, and the rabbit-derived lactobacillus is separated, which can regulate the balance of intestinal microorganisms. When used in combination with the above-mentioned complete feed, it can relieve the weaning stress of young rabbits and improve intestinal health. Fresh chyme is the content in the intestinal tract. The lactic acid bacteria extracted from fresh chyme and extracted from the intestinal mucosa of rabbits are homologous bacteria.

[0028] The lanthanum-containing rabbit-derived lactobacillus separated and purified in the present invention has the same effect on the original bacteria and the cultured offspring. If the original bacteria are lost, they can be re-obtained by the above method, which is convenient to obtain, low in cost and high in stability.

[0029] The drug-free formula of the composite microecological complete feed in the present invention is suitable for weaned rabbits (weaned rabbits of about 35 days old) and can be directly fed to improve and regulate the intestinal morphology and alleviate the problem of diarrhea in weaned rabbits. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a scanning electron microscope image of a tungsten filament of Lactobacillus plantarum in the present invention.

[0031] Figure 2 This is a scanning electron microscope image of the tungsten filament of the lanthanum-enriched plant lactobacillus in the present invention.

[0032] Figure 3 This is the effect of different forms of Lactobacillus plantarum in the present invention on the Alpha diversity index of the intestinal flora of weaned rabbits. DETAILED DESCRIPTION

[0033] The present invention is further described below in conjunction with specific embodiments:

[0034] Example 1

[0035] A weaned rabbit feed additive, comprising the following components and dosages: rabbit-derived plant lactobacillus, rabbit-derived plant lactobacillus containing lanthanum and vitamin C, wherein the addition amount of each raw material is 10 8 -10 13 cfu, vitamin C 100mg.

[0036] The preparation method is to prepare various bacteria and vitamin C into coated freeze-dried powder and then mix them evenly. The number of viable bacteria of each bacteria in the freeze-dried powder is maintained at 10 10 -10 11 cfu / g.

[0037] Rabbit-derived Lactobacillus plantarum and lanthanum-containing rabbit-derived Lactobacillus plantarum are new endogenous lactic acid bacteria in the rabbit intestine, which are extracted from fresh chyme collected from the rabbit intestine within 30 minutes.

[0038] The culture medium used in the extraction process of rabbit-derived Lactobacillus plantarum is MRS solid culture medium and Lactobacillus selective culture medium.

[0039] In g / L, the lactobacillus selective culture medium has the following components:

[0040] Peptone 10, bean extract powder 10, yeast extract powder 2, sodium acetate 5, triammonium citrate 2, magnesium sulfate 0.2, manganese sulfate 0.05, Tween 80, glucose 20, calcium carbonate 5, agar 25, pH value 6-6.5.

[0041] The novel lanthanum-containing rabbit-derived Lactobacillus plantarum is composed of the following MRS solid culture medium components (g / L):

[0042] Peptone 10, beef powder 8, yeast powder 4, glucose 20, dipotassium hydrogen phosphate 2, diammonium hydrogen citrate 2, sodium acetate 5, magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1, pH value 5.5-5.9, lanthanum chloride heptahydrate, 0.13-0.15.

[0043] Example 2

[0044] The complete drug-free formula of the composite microecological additive for weaned rabbit feed in Example 1 is composed of the following components and amounts:

[0045] Complete feed ingredients (%): wheat flour 15.272, glucose 5, vitamins and minerals 0.35, soybean meal 14.78, rice bran 16, alfalfa 45.56, vitamin C 0.01, calcium bicarbonate 0.1, bentonite 0.708, calcium carbonate 0.35, sodium chloride 0.25, soybean oil 1.43, methionine 0.14, choline chloride 0.05, new lanthanum-containing rabbit-derived Lactobacillus plantarum 10 8 -10 13 cfu / kg, Lactobacillus plantarum from rabbit 10 8 -10 13 cfu / kg and vitamin C 100mg.

[0046] Vitamins and minerals per kilogram: Vitamin A, 10,000 IU; Vitamin D3, 1,200 IU; DL-α-tocopherol, 40 mg; Vitamin K3, 2 mg; Biotin, 0.4 mg; Niacin, 50 mg; Vitamin B7, 4 mg; Thiamine, 4 mg, Riboflavin, 6 mg; Cobalamin, 0.2 mg; Calcium pantothenate, 20 mg; Folic acid, 5 mg; Fe (FeSO4·H2O), 50 mg; Cu CuSO4·5H2O), 10 mg; Mn (MnSO4·H2O), 8.5 mg; Co (CoSO4·7H2O), 0.25 mg; I (KI), 0.2 mg; Zn (ZnSO4·H2O), 50 mg; Se (Na2SeO3), 0.05 mg.

[0047] The preparation method comprises the following specific steps:

[0048] (1) preparing rabbit-derived Lactobacillus plantarum, lanthanum-containing rabbit-derived Lactobacillus plantarum and vitamin C into coated freeze-dried powders and packaging them into bags;

[0049] (2) Weighing and mixing other raw materials evenly and packaging them into individual bags;

[0050] (3) When feeding, add the freeze-dried powder to the other raw materials in step (2) under the conditions of an ambient temperature of 15-25° C. and an ambient humidity of 50±3% and mix well.

[0051] Example 3 Obtaining rabbit-derived Lactobacillus plantarum and lanthanum-containing rabbit-derived Lactobacillus plantarum

[0052] (1) Preliminary experiment: 0.1 g of fresh chyme (collected within 30 minutes) of rabbit cysts was taken with an inoculating loop and transferred to 0.9 mL of sterile distilled water. The remaining sample was quickly returned to a -80°C freezer for storage. Shake well on a homogenizer for 30 seconds and perform a 10-fold gradient dilution to 1×10 -8 , use a pipette to draw 1×10 -4 , 1×10 -5 , 1×10 -6 , 1×10 -7 , 1×10 -8 100 μL of each dilution was evenly spread on the solid culture medium.

[0053] The plate was placed in a carbon dioxide incubator (15%, 37°C) and incubated upside down for 48 hours.

[0054] (2) Preparation of solid culture medium (MRS and Lactobacillus selective culture medium)

[0055] MRS solid culture medium ingredients (g / L): peptone, 10; beef powder, 8; yeast powder, 4; glucose, 20; dipotassium hydrogen phosphate, 2; diammonium hydrogen citrate, 2; sodium acetate, 5; magnesium sulfate, 0.2; manganese sulfate 0.04; Tween 80, 1; pH value 5.7±0.2 (25℃).

[0056] Components of the lactobacillus selective culture medium (g / L): peptone, 10; bovine extract powder, 10; yeast extract powder, 2; sodium acetate, 5; triammonium citrate, 2; magnesium sulfate, 0.2; manganese sulfate 0.05; Tween 80; glucose, 20; calcium carbonate, 5; agar, 25; pH value 6.25±0.25 (25℃).

[0057] (3) Turn on the ultraviolet light of the clean bench for 30 minutes, and wipe the equipment that needs to be placed in the clean bench with 75% alcohol. Wrap the culture medium, calcium carbonate, distilled water, culture dishes, test tubes, gun tips, EP tubes, etc. in newspapers and place them in a high-temperature and high-pressure sterilizer at 121℃ for 30 minutes. After taking them out, place them on the clean bench to cool.

[0058] (4) When the temperature is about 50°C, mix the solid culture medium evenly and pour it into a plate. Place it on a clean bench and wait for it to cool and solidify.

[0059] (5) Use an inoculating loop to take 0.1 g of fresh chyme from rabbit cysts (collected within 30 minutes) and put it into a 1.5 ml EP tube. Slowly add 0.9 mL of sterile distilled water to the EP tube, and quickly put the rest of the sample back into a -80 °C freezer for storage. Shake the EP tube on a mixer for 30 seconds, and perform a 10-fold gradient dilution to 1×10 -8 According to the preliminary test results, dilutions with a concentration gradient of 30-300 viable bacteria (1×10 -4 , 1×10 -5 , 1×10 -6 ) 100 μL each in solid culture medium and Lactobacillus selective culture medium, and make 2 plates for each dilution. The plates were placed in a carbon dioxide incubator (15%, 37°C) and inverted for 48 hours.

[0060] (6) After taking out the plates, select colonies with the characteristics of lactobacillus colonies on a clean bench for plate counting and record the number of live lactobacillus bacteria on each plate.

[0061] (7) Use an inoculation loop to pick up lactobacillus colonies with different colony shapes on the surface, inoculate different colony shapes into test tubes containing MRS liquid culture medium, and expand and culture them in a carbon dioxide incubator (15%, 37°C) for 48 hours.

[0062] (8) Repeat steps (2) to (5) using the culture medium obtained in step (7) as a sample.

[0063] (9) Select the colonies obtained in step (8) for Gram staining and microscopic examination. After confirming that the Gram-positive strain is a pure strain, repeat step (7) to expand it again.

[0064] (10) Spread the culture solution obtained in step (9) onto a disposable sterile culture dish according to steps (2) to (5), incubate the dish upside down in a carbon dioxide incubator (15%, 37°C) for 48 hours, seal the dish with tape, and send it for inspection.

[0065] (11) The intestinal bacterial samples separated and purified above were identified by bacterial species (comparison of 16S rDNA sequences of bacterial ribosomes). When the homology of 16S rDNA sequences was higher than 97%, they could be considered to be the same species of bacteria within the genus.

[0066] After identification, the rabbit intestinal Lactobacillus plantarum obtained this time, such as Figure 1 As shown. Rabbit-derived Lactobacillus plantarum, with a smooth surface.

[0067] (12) Lactobacillus plantarum was directly mass-produced to obtain freeze-dried Lactobacillus plantarum powder (5.2×10 10 cfu / g; Lanthanum oxide heptahydrate was added to the culture medium for mass production to obtain lanthanum-containing Lactobacillus plantarum freeze-dried powder (6×10 10 cfu / g), the obtained lanthanum-rich rabbit-derived Lactobacillus plantarum Figure 2 As shown, the surface is not smooth due to the enrichment of many lanthanum ions, and the surface shows obvious granularity. Adding lanthanum oxide can promote the production of lactobacillus and reduce production costs.

[0068] Example 4

[0069] Plant Lactobacillus was made into two forms of coated freeze-dried powder, single and new active lanthanum-rich plant Lactobacillus, as a drug-free formula for the composite microecological complete feed for weaned rabbits. The number of live bacteria per gram of each bacterium in the freeze-dried powder was maintained at 10 10 -10 11 cfu / gt and vitamin C 100mg, evenly added into the complete feed of weaned rabbits, feeding them can improve and regulate intestinal health.

[0070] It is directly involved in the formation of the digestive tract membrane flora and forms a specific reversible bond with the rabbit intestinal mucosal receptors, which not only strengthens the mechanical barrier but also constitutes a biological barrier. It plays an important role in preventing the colonization of pathogenic bacteria and conditional pathogens. It also has a restraining effect on the colonized bacteria, thereby maintaining the microecological balance of the intestine. It has the functions of vitamin and protein synthesis and assisting digestion and absorption, which can prevent the invasion of external bacteria, stimulate immune function, and maintain the health of the body. Experiment 1: Animal experiment of the drug-free formula of the composite microecological complete feed for weaned rabbits

[0071] Under the same conditions, there are experimental group and control group.

[0072] The rabbits were weaned at 35 days of age, and the feeding experiment lasted for 28 days after weaning. They were free to eat and drink water. After the experiment, the production performance, duodenal intestinal morphology, and the expression level of tight junction protein gene mRNA between epithelial cells and organ index of the weaned rabbits were measured 3-4 weeks after weaning.

[0073] The results showed that compared with the control group, the growth performance of rabbits could be significantly improved, and the expression of tight junction protein gene mRNA between duodenal epithelial cells was significantly affected, as shown in Tables 1 to 4 below.

[0074] The test results are shown in Tables 1 to 4 and Figure 3 ( Figure 3It is another separate measurement indicator, which shows the effect of Lactobacillus plantarum on the diversity of rabbit intestinal microorganisms), where P value <0.01 indicates a significant difference (P value is P-VALUE, used in significance analysis); CON represents the control group, to which Lactobacillus plantarum and lanthanum-enriched Lactobacillus plantarum are not added; L represents the complete feed with the addition of rabbit-derived Lactobacillus plantarum; L+La represents the complete feed with the addition of lanthanum-enriched rabbit-derived Lactobacillus plantarum; LO represents heterologous Lactobacillus plantarum.

[0075] Table 1 Effects of different forms of Lactobacillus plantarum on the production performance of weaned rabbits

[0076] project con L L+la P-value Average daily weight gain, g 39.98 36.79 37.38 0.269 Average daily feed intake, g 140.85 140.44 135.57 0.569 Weight gain meat feed ratio 0.27a 0.25b 0.27a 0.089

[0077] Comparing the L and L+la groups in Table 1, it is found that the weight gain to meat feed ratio of rabbits fed with lanthanum-enriched rabbit-derived Lactobacillus plantarum is better than that of the Lactobacillus plantarum group. Numerically, lanthanum-enriched rabbit-derived Lactobacillus plantarum is also better than the Lactobacillus plantarum group.

[0078] Table 2 Effects on the mRNA expression of tight junction proteins between duodenal epithelial cells of weaned rabbits

[0079] project con L L+la P-value claudin-1 0.11367 0.11767 0.14650 0.7408 claudin-10 0.1185b 0.1713b 0.4508a 0.0251 occludin 0.11850b 0.16683b 0.33567a 0.0033 zonula-occludens 0.11550b 0.14533b 0.28750a 0.0013 interleukin-10 0.05167b 0.04850b 0.09717a 0.0427

[0080] It can be seen from Table 2 that the lanthanum-rich rabbit-derived Lactobacillus plantarum significantly increased the mRNA expression of tight junction proteins between duodenal epithelial cells of weaned rabbits, indicating that the lanthanum-rich rabbit-derived Lactobacillus plantarum can improve the connection state between duodenal epithelial cells.

[0081] Table 3 Effects of different forms of Lactobacillus plantarum on organ indexes of weaned rabbits

[0082] project,% con L L+la P-value heart 0.23000b 0.25767a 0.25750a 0.0767 Cecal vermiform process 0.31733b 0.38833a 0.36933a 0.0970

[0083] As shown in Table 3, both lanthanum-enriched rabbit-derived Lactobacillus plantarum and rabbit-derived Lactobacillus plantarum can improve the organ indexes of the heart and cecal vermiform process. Among them, the cecal vermiform process is an important immune organ unique to rabbits and plays an important role in intestinal health.

[0084] Table 4 Effects of homologous and heterologous Lactobacillus plantarum on cardiac index, weight gain-to-meat ratio and serum immunoglobulin content in weaned rabbits

[0085] project con L LO P-value Cardiac index, % 0.23000b 0.25767a 0.230b 0.0817 Weight gain to feed ratio 0.269000a 0.251500a 0.273b <0.0001

[0086] As can be seen from Table 4, compared with heterologous Lactobacillus plantarum, homologous Lactobacillus plantarum can significantly improve the production performance and cardiac organ index of weaned rabbits (LO in Table 4 is heterologous Lactobacillus plantarum, and L is homologous Lactobacillus plantarum).

[0087] It can be seen from Tables 1-4 above that after the lanthanum-enriched rabbit-derived Lactobacillus plantarum of the present invention is added to the complete feed, the production performance and intestinal health of meat rabbits are improved.

[0088] The above implementation / test examples are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A formula for a composite microecological complete feed for weaned rabbits, characterized in that: The complete feed ingredients include the following ingredients in unit %: wheat flour 15.272, glucose 5, vitamins and minerals 0.35, soybean meal 14.78, rice bran 16, alfalfa 45.56, vitamin C 0.01, calcium bicarbonate 0.1, bentonite 0.708, calcium carbonate 0.35, sodium chloride 0.25, soybean oil 1.43, methionine 0.14, choline chloride 0.05, and composite microecological additives; wherein, per kilogram of complete feed, the composite microbial additive includes 10 lanthanum-containing rabbit-derived plant lactobacillus. 8 -10 13 cfu / kg, Lactobacillus plantarum from rabbit 10 8 -10 13 cfu / kg and vitamin C 100mg.

2. A weaned rabbit composite microecological complete feed formula according to claim 1, characterized in that: The rabbit-derived Lactobacillus plantarum and the lanthanum-containing rabbit-derived Lactobacillus plantarum are novel endogenous lactic acid bacteria in rabbit intestines, and are extracted from fresh chyme collected from rabbit intestines within 30 minutes.

3. A weaned rabbit composite microecological complete feed formula according to claim 2, characterized in that: The culture medium used in the rabbit-source plant lactobacillus extraction process is an MRS solid culture medium or a lactobacillus selective culture medium.

4. A weaned rabbit composite microecological complete feed formula according to claim 3, characterized in that: The unit is g / L. The composition of the lactobacillus selective culture medium is as follows: Peptone 10, bean extract powder 10, yeast extract powder 2, sodium acetate 5, triammonium citrate 2, magnesium sulfate 0.2, manganese sulfate 0.05, Tween 80, glucose 20, calcium carbonate 5, agar 25, pH value 6-6.

5.

5. A weaned rabbit composite microecological complete feed formula according to claim 3, characterized in that: The unit is g / L. The components of the MRS solid culture medium are as follows: peptone 10, beef powder 8, yeast powder 4, glucose 20, dipotassium hydrogen phosphate 2, diammonium hydrogen citrate 2, sodium acetate 5, magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1, pH value 5.5-5.9, lanthanum chloride heptahydrate 0.13-0.

15.

6. A drug-free formula for weaned rabbit composite microecological complete feed according to any one of claims 1-5, characterized in that: The vitamins and minerals per kilogram are as follows: Vitamin A, 10,000 IU; Vitamin D3, 1,200 IU; DL-α-tocopherol, 40 mg; Vitamin K3, 2 mg; Biotin, 0.4mg; Niacin, 50mg; Vitamin B7, 4mg; Thiamine, 4mg, riboflavin, 6mg; cobalamin, 0.2mg; calcium pantothenate, 20mg; folic acid, 5mg; Fe(FeSO4·H2O), 50mg; Cu(CuSO4·5H2O), 10mg; Mn(MnSO4·H2O), 8.5mg; Co(CoSO4·7H2O), 0.25mg; I(KI), 0.2mg; Zn(ZnSO4·H2O), 50mg; Se(Na2SeO3), 0.05mg.

7. The method for preparing a composite microecological complete feed formula for weaned rabbits according to claim 1, characterized in that: (1) preparing rabbit-derived Lactobacillus plantarum, lanthanum-containing rabbit-derived Lactobacillus plantarum and vitamin C into coated freeze-dried powders and packaging them into bags; (2) Weighing and mixing other raw materials evenly and packaging them into individual bags; (3) When feeding, add the freeze-dried powder to the other raw materials in step (2) under the conditions of an ambient temperature of 15-25° C. and an ambient humidity of 50±3% and mix well.

8. The method for preparing a drug-free formula for a composite microecological complete feed for weaned rabbits according to claim 7, characterized in that: The number of viable bacteria per gram of freeze-dried powder of each bacterium in step (1) is 10 10 -10 11 cfu / g.

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