Mandarin fish compound feed with low fish meal content as well as preparation method and application of mandarin fish compound feed

By using chicken powder and dephenolized cottonseed protein powder to replace fish meal in mandarin fish feed, the problem of high demand for fish meal content of mandarin fish is solved, the breeding cost is reduced, the feed utilization rate is improved, and the economic and functional mandarin fish feed is achieved. Continuous development.

CN119404985APending Publication Date: 2025-02-11ZHUHAI HAILONG BIOLOGICAL TECH CO LTD
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Patent Information

Application Number
CN202411736498.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-29
Publication Date
2025-02-11

AI Technical Summary

Technical Problem

Mandarin fish has a high demand for fish meal content, and the price of fish meal has risen and the supply is tight, resulting in an increase in breeding costs and affecting the sustainable development of the industry.

Method used

Use chicken powder and dephenified cottonseed protein powder to replace some fish meal in mandarin fish feed, reduce the amount of fish meal and improve the protein content and nutritional balance of the feed.

Benefits of technology

It effectively reduces the use of fish meal, reduces the feed cost of mandarin fish farming, and ensures the growth performance and feed utilization rate of mandarin fish, alleviates the tight supply of fish meal.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention belongs to the technical field of fish feeds, discloses a low-fish-meal mandarin fish compound feed as well as a preparation method and application thereof, and particularly discloses the low-fish-meal mandarin fish compound feed. Comprising the following components: fish meal, chicken meal, pig spray-dried animal blood cells, shrimp meal, dephenolized cottonseed protein powder, starch, soybean oil, monocalcium phosphate, microcrystalline cellulose, premix, rice hull powder and amino acid. In the components of the low-fish-meal mandarin fish compound feed provided by the invention, a proper amount of chicken meal and dephenolized cottonseed protein powder are used for replacing fish meal, so that the growth performance of mandarin fish can be improved. Therefore, a proper amount of chicken meal and dephenolized cottonseed protein powder are used for replacing fish meal in the mandarin fish feed, so that the breeding cost can be obviously reduced without reducing the production efficiency, and the healthy and sustainable development of the industry is promoted while the economic benefit is improved. Lysine, methionine and threonine are supplemented, and the problem of amino acid imbalance caused by replacement of fish meal with chicken meal in the feed is solved.
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Description

Technical Field

[0001] The invention belongs to the technical field of fish feed, and particularly relates to a low-fish meal mandarin fish compound feed and a preparation method and application thereof. Background Art

[0002] Mandarin fish (Siniperca chuatsi) belongs to the order Perciformes, subfamily Sinipernae, genus Siniperca, commonly known as Mandarin fish, Mandarin fish, and seasonal flower fish, and is a special and precious freshwater fish in my country. Mandarin fish is a ferocious carnivorous fish with a very special diet. It only eats live fish throughout its life cycle. It is extremely difficult to domesticate it to accept artificial compound feeds. This phenomenon is rare in the breeding of aquatic economic fish. After years of research, Chinese experts have successfully determined an effective and stable method for training mandarin fish to eat artificial feeds. However, mandarin fish has a high demand for fish meal and protein content in feed. The amount of fish meal in commercial feed generally reaches more than 50%. Too low fish meal content will cause mandarin fish to spit out feed, reduce food intake, or even close its mouth. In severe cases, it will cause mandarin fish to become ill or even die. In recent years, the vigorous development of intensive breeding has led to a significant increase in the demand for fish meal, and the increasing shortage of marine fishery resources in recent years has also led to a continuous increase in the price of fish meal. Chicken meal is made from scraps, residual minced meat and viscera produced during the processing of chicken products. It has attracted much attention from the animal protein source production market due to its rich nutritional value (more than 60% crude protein, about 10% crude fat), high digestibility, good amino acid balance and high bioconversion rate. The special feeding habits of mandarin fish determine that the effect of feed raw materials that work well in other fish in mandarin fish feed is unpredictable. Therefore, in order to alleviate the current tight supply of fish meal, reduce breeding costs, and promote the healthy and sustainable development of the industry, replacing part of the fish meal in the mandarin fish feed formula is an important task worthy of research and application in feed mandarin fish breeding. Summary of the invention

[0003] The present invention aims to provide a low-fishmeal mandarin fish compound feed and a preparation method and application thereof. Reasonably using chicken meal and dephenolized cottonseed protein meal to replace part of the fish meal in mandarin fish feed not only reduces the amount of fish meal, saves fish meal resources, but also reduces the feed cost of mandarin fish farming, but does not affect the feeding and growth of mandarin fish.

[0004] The first aspect of the present invention aims to provide a low-fishmeal mandarin fish feed.

[0005] The purpose of the second aspect of the present invention is to provide a method for preparing the low-fish meal mandarin fish compound feed according to the first aspect of the present invention.

[0006] The third aspect of the present invention aims to provide the use of the low-fishmeal mandarin fish compound feed of the first aspect of the present invention in fish farming.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] The first aspect of the present invention provides a low-fishmeal mandarin fish compound feed, comprising the following ingredients: fish meal, chicken meal, porcine hemoglobin powder, shrimp meal, dephenolized cottonseed protein powder, starch, soybean oil, calcium dihydrogen phosphate, microcrystalline cellulose, premix, rice husk powder and amino acids.

[0009] In some embodiments of the present invention, the low fish meal mandarin fish feed includes, by weight, 20-55 parts of fish meal, 15-55 parts of chicken meal, 1-6 parts of porcine hemoglobin powder, 1-4 parts of shrimp meal, 2-8 parts of dephenolized cottonseed protein powder, 8-15 parts of starch, 1-6 parts of soybean oil, 1-3 parts of monocalcium phosphate, 0.5-3 parts of microcrystalline cellulose, 1-4 parts of premix, 0.1-1 parts of rice husk powder and 0.1-1 parts of amino acids.

[0010] In some embodiments of the present invention, the low fish meal mandarin fish feed includes, by weight, 23-50 parts of fish meal, 15-50 parts of chicken meal, 1-5 parts of porcine hemoglobin powder, 1-3 parts of shrimp meal, 3-6 parts of dephenolized cottonseed protein powder, 9-13 parts of starch, 1-5 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.5-2.5 parts of microcrystalline cellulose, 1-3 parts of premix, 0.3-1 parts of rice husk powder and 0.1-0.8 parts of amino acids.

[0011] The invention can reduce the amount of fish meal used in mandarin fish feed by using chicken meal, and the minimum amount of fish meal used can be as low as 23%. Using an appropriate amount of chicken meal and dephenolized cottonseed protein to replace fish meal in mandarin fish feed will not affect the feeding of mandarin fish, and can meet the nutritional needs of mandarin fish growth, alleviate the current tight supply of imported fish meal, and improve feed utilization efficiency.

[0012] In some embodiments of the present invention, the low fish meal mandarin fish feed includes, by weight, 25-50 parts of fish meal, 15-40 parts of chicken meal, 2-4 parts of porcine hemoglobin powder, 2-3 parts of shrimp meal, 4-6 parts of dephenolized cottonseed protein powder, 9-11 parts of starch, 2-5 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.5-2 parts of microcrystalline cellulose, 2-3 parts of premix, 0.5-0.9 parts of rice husk powder and 0.1-0.6 parts of amino acids.

[0013] In some embodiments of the present invention, the starch used includes cassava starch, and the starch content is greater than or equal to 85%.

[0014] In some embodiments of the present invention, the amino acid includes at least one of lysine, methionine and threonine to ensure the balance of amino acids in the feed and improve the utilization rate of protein in the feed by the fish. The added amount of each amino acid is 0.1wt% to 0.5wt% of lysine, 0.05wt% to 0.2wt% of methionine, and 0.01wt% to 0.05wt% of threonine.

[0015] In some embodiments of the present invention, the chicken meal is selected from pet-grade chicken bone meal, and its protein content is greater than or equal to 65%. The protein content of cottonseed protein powder is greater than or equal to 60%, and must be treated with cottonseed phenol removal.

[0016] In some embodiments of the present invention, the porcine blood globulin powder is spray-dried porcine blood globulin powder.

[0017] In some embodiments of the present invention, the shrimp meal is Antarctic krill meal.

[0018] In some embodiments of the present invention, the premix comprises vitamins, minerals, sodium chloride, antioxidants and preservatives.

[0019] In some embodiments of the present invention, the premix includes, by weight, 1 to 2 parts of vitamins, 1 to 2 parts of minerals, 0.1 to 0.2 parts of sodium chloride, 0.01 to 0.02 parts of antioxidants, and 0.05 to 0.1 parts of preservatives.

[0020] In some embodiments of the present invention, the vitamins include: choline, vitamin A, vitamin D, vitamin E, vitamin K, riboflavin, vitamin B6, pantothenic acid, niacin, biotin, vitamin B12, folic acid, vitamin C; and / or, the minerals include: calcium bicarbonate, potassium chloride, magnesium sulfate, cupric sulfate pentahydrate, ferrous sulfate, zinc sulfate, manganese sulfate, sodium selenate, potassium iodide, sodium molybdate, cobalt sulfate and potassium fluoride.

[0021] In some embodiments of the present invention, the antioxidant includes at least one of ethoxyquin, ethoxyquinoline, butylated hydroxytoluene, butylated hydroxyanisole and tert-butylhydroquinone; preferably ethoxyquinoline.

[0022] In some embodiments of the present invention, the preservative includes at least one of propionic acid, calcium propionate, sodium diacetate, calcium carbonate, dimethyl fumarate, benzoic acid and paraben; preferably propionic acid.

[0023] In some embodiments of the present invention, the protein content of the low fish meal mandarin fish compound feed is 52% to 56%, and the fat content is 10% to 14%.

[0024] The low-fishmeal mandarin fish compound feed can be used for the cultivation of mandarin fish weighing more than 30g, with two meals per day, and the daily feeding rate is about 2.5% to 3.5% of the mandarin fish body weight. It can reduce the amount of fishmeal used in the feed, reduce the feed cost, and maintain the growth rate of the mandarin fish and the feed utilization rate.

[0025] The second aspect of the present invention provides a method for preparing the low fish meal mandarin fish compound feed of the first aspect of the present invention, comprising the following steps: mixing fish meal, chicken meal, porcine hemoglobin powder, shrimp meal, dephenolized cottonseed protein powder, starch, calcium dihydrogen phosphate, microcrystalline cellulose, premix, rice husk powder and amino acids, conditioning, puffing and granulating, and drying to obtain a semi-finished feed; and spraying soybean oil on the surface of the semi-finished feed to obtain a low fish meal mandarin fish compound feed.

[0026] In some embodiments of the present invention, fish meal, chicken meal, porcine hemoglobin powder, shrimp meal, dephenolized cottonseed protein powder, starch, calcium dihydrogen phosphate, microcrystalline cellulose, premix, rice husk powder and amino acid are mixed and ultrafinely ground, and then passed through a sieve with a mesh size of 80 to 100.

[0027] In some embodiments of the present invention, the particle size:length of the low fish meal mandarin fish feed is 1:3-4.

[0028] The third aspect of the present invention provides the use of the low-fishmeal mandarin fish compound feed of the first aspect of the present invention in fish farming.

[0029] In some embodiments of the present invention, the fish includes mandarin fish.

[0030] The beneficial effects of the present invention are:

[0031] In the components of the low-fishmeal mandarin fish compound feed provided by the present invention, after using an appropriate amount of chicken meal and dephenolized cottonseed protein powder to replace fish meal, the growth performance of mandarin fish can be improved. Therefore, using an appropriate amount of chicken meal and dephenolized cottonseed protein powder to replace fish meal in mandarin fish feed can significantly reduce breeding costs without reducing production efficiency, improve economic benefits and promote healthy and sustainable development of the industry. Lysine, methionine and threonine are supplemented, solving the problem of amino acid imbalance caused by replacing fish meal with chicken meal in feed.

[0032] A small amount of dephenolized cottonseed protein powder was added to the ingredients of mandarin fish feed. The results showed that appropriate and specially treated plant protein did not affect the feeding and growth of mandarin fish. Therefore, it broke the traditional view that mandarin fish is extremely intolerant to plant protein, and provided important reference significance for the subsequent upgrade of mandarin fish feed formula. DETAILED DESCRIPTION

[0033] The present invention is further described in detail below through specific examples.

[0034] It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0035] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0036] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0037] Example 1

[0038] A low-fishmeal mandarin fish feed comprises the following raw materials, measured by weight: 23 parts of fish meal, 49.5 parts of chicken meal, 3 parts of spray-dried porcine hemoglobin powder, 2 parts of Antarctic krill meal, 3 parts of dephenolized cottonseed protein powder, 10.2 parts of cassava starch, 1.6 parts of soybean oil, 1.5 parts of calcium dihydrogen phosphate, 2.19 parts of microcrystalline cellulose, 1.4 parts of vitamin premix, 1.35 parts of mineral premix, 0.1 part of sodium chloride, 0.02 parts of ethoxyquin, 0.08 parts of propionic acid, 0.38 parts of rice husk powder, 0.47 parts of lysine, 0.18 parts of DL-methionine and 0.03 parts of threonine.

[0039] In the raw materials of the low-fishmeal mandarin fish compound feed, the protein content of chicken meal is 66.5%, and the content of dephenolized cottonseed protein is 61.5%.

[0040] The vitamin premix includes (content per kilogram of finished feed): 1000 mg of choline, 40 mg of vitamin A, 60 μg of vitamin D, 200 mg of vitamin E, 10 mg of vitamin K, 25 mg of riboflavin, 20 mg of vitamin B6, 50 mg of pantothenic acid, 200 mg of niacin, 3.2 mg of biotin, 0.1 mg of vitamin B12, 10 mg of folic acid, and 210 mg of vitamin C.

[0041] The mineral premix includes (content per kilogram of finished feed): 15g calcium bicarbonate, 3g potassium chloride, 0.8g magnesium sulfate, 10mg copper sulfate pentahydrate, 150mg ferrous sulfate, 80mg zinc sulfate, 13mg manganese sulfate, 0.7mg sodium selenate, 1.1mg potassium iodide, 0.14mg sodium molybdate, 0.02mg cobalt sulfate, and 0.26mg potassium fluoride.

[0042] The method for preparing the low-fishmeal mandarin fish compound feed comprises the following steps:

[0043] (1) Mix the raw materials except soybean oil and stir them evenly;

[0044] (2) After the mixture in (1) is ultrafinely ground, a sieve with a mesh size of 80 to 100 is used to remove large particles of impurities;

[0045] (3) The sieved raw materials in (2) are subjected to conditioning, puffing, granulation, and drying (see “Li Hailing. Feed processing process [J]. Rural Science and Technology, 2016, (24): 39.” and “Qin Yi, Wang Jinrong, Qiao Hanzhen, et al. Effect of processing technology on feed nutritional quality [J]. Feed Research, 2019, 42 (12): 83-86.”, the same below) to obtain a semi-finished feed product;

[0046] (4) After the semi-finished feed obtained in (3) is cooled, soybean oil is evenly sprayed on the surface of the semi-finished feed to obtain a low-fish meal mandarin fish feed.

[0047] The low-fishmeal mandarin fish compound feed obtained in this embodiment has a protein content of 55.10% and a fat content of 11.40%. The obtained feed particle size is 4.58±0.25 mm and the length is 15.67±0.72 mm. The low-fishmeal mandarin fish compound feed is added with lysine sulfate, DL-methionine and threonine, and combined with other ingredients, it can improve the nutritional balance and palatability of the feed and fully meet the growth needs of mandarin fish.

[0048] Example 2

[0049] A low-fishmeal mandarin fish feed comprises the following raw materials, measured by weight: 31.5 parts of fish meal, 39.5 parts of chicken meal, 3 parts of spray-dried porcine hemoglobin powder, 2 parts of Antarctic krill meal, 4 parts of dephenolized cottonseed protein powder, 10.1 parts of cassava starch, 2.5 parts of soybean oil, 1.5 parts of calcium dihydrogen phosphate, 1.89 parts of microcrystalline cellulose, 1.4 parts of vitamin premix, 1.35 parts of mineral premix, 0.1 part of sodium chloride, 0.02 parts of ethoxyquin, 0.08 parts of propionic acid, 0.55 parts of rice husk powder, 0.35 parts of lysine, 0.14 parts of DL-methionine and 0.02 parts of threonine.

[0050] The raw material sources and protein content of the low-fishmeal mandarin fish compound feed and the preparation method of the feed are the same as those in Example 1.

[0051] The low-fishmeal mandarin fish compound feed obtained in this embodiment has a protein content of 55.07% and a fat content of 11.51%. The obtained feed particle size is 4.65±0.39 mm and the length is 15.25±0.96 mm. The low-fishmeal mandarin fish compound feed is added with lysine sulfate, DL-methionine and threonine, and combined with other ingredients, it can improve the nutritional balance and palatability of the feed and fully meet the growth needs of mandarin fish.

[0052] Example 3

[0053] A low-fishmeal mandarin fish feed comprises the following raw materials, measured by weight: 40.5 parts of fish meal, 29.5 parts of chicken meal, 3 parts of spray-dried porcine hemoglobin powder, 2 parts of Antarctic krill meal, 5 parts of dephenolized cottonseed protein powder, 10 parts of cassava starch, 3.3 parts of soybean oil, 1.5 parts of monocalcium phosphate, 1.2 parts of microcrystalline cellulose, 1.4 parts of vitamin premix, 1.35 parts of mineral premix, 0.1 part of sodium chloride, 0.02 parts of ethoxyquin, 0.08 parts of propionic acid, 0.7 parts of rice husk powder, 0.24 parts of lysine, 0.09 parts of DL-methionine and 0.02 parts of threonine.

[0054] The raw material sources and protein content of the low-fishmeal mandarin fish compound feed and the preparation method of the feed are the same as those in Example 1.

[0055] The low-fishmeal mandarin fish compound feed obtained in this embodiment has a protein content of 55.59% and a fat content of 11.80%. The obtained feed particle size is 4.38±0.45 mm and the length is 15.42±0.83 mm. The low-fishmeal mandarin fish compound feed is added with lysine sulfate, DL-methionine and threonine, and combined with other ingredients, it can improve the nutritional balance and palatability of the feed, and fully meet the growth needs of mandarin fish.

[0056] Example 4

[0057] A low-fishmeal mandarin fish feed comprises the following raw materials, measured by weight: 49 parts of fish meal, 19.5 parts of chicken meal, 3 parts of spray-dried porcine hemoglobulin powder, 2 parts of Antarctic krill meal, 6 parts of dephenolized cottonseed protein powder, 10 parts of cassava starch, 4.2 parts of soybean oil, 1.5 parts of calcium dihydrogen phosphate, 0.79 parts of microcrystalline cellulose, 1.4 parts of vitamin premix, 1.35 parts of mineral premix, 0.1 part of sodium chloride, 0.02 parts of ethoxyquin, 0.08 parts of propionic acid, 0.88 parts of rice husk powder, 0.12 parts of lysine, 0.05 parts of methionine and 0.01 parts of threonine.

[0058] The raw material sources and protein content of the low-fishmeal mandarin fish compound feed and the preparation method of the feed are the same as those in Example 1.

[0059] The low-fishmeal mandarin fish compound feed obtained in this embodiment has a protein content of 55.53% and a fat content of 11.45%. The obtained feed particle size is 4.85±0.36 mm and the length is 15.75±0.68 mm. The low-fishmeal mandarin fish compound feed is added with lysine sulfate, DL-methionine and threonine, and combined with other ingredients, it can improve the nutritional balance and palatability of the feed and fully meet the growth needs of mandarin fish.

[0060] Comparative Example 1

[0061] A common mandarin fish compound feed comprises the following raw materials, measured by weight: 64 parts of fish meal, 10.5 parts of chicken meal, 3 parts of spray-dried porcine hemoglobulin powder, 2 parts of Antarctic krill meal, 10 parts of cassava starch, 5 parts of soybean oil, 1.5 parts of calcium dihydrogen phosphate, 1.4 parts of vitamin premix, 1.35 parts of mineral premix, 0.1 part of sodium chloride, 0.02 parts of ethoxyquinoline, 0.08 parts of propionic acid and 1.05 parts of rice husk powder.

[0062] The raw material sources and protein content of the above-mentioned common mandarin fish compound feed and the preparation method of the feed are the same as those in Example 1.

[0063] The results show that the protein content of the mandarin fish feed obtained in this comparative example is 55.54%, the fat content is 11.50%, and the particle size of the obtained feed is 4.74±0.16 mm and the length is 15.64±0.78 mm.

[0064] Effect Example

[0065] This verification experiment was conducted at the Dixuan Experimental Base of Haida Group in Guangzhou. The mandarin fish that were stably fed with feed were randomly divided into 5 groups (3 replicates in each group, 300 mandarin fish in each replicate), and were fed the feeds of Comparative Example 1 and Examples 1 to 4, respectively.

[0066] The size of the experimental cage was 2m×3m×2m. The fish were fed twice a day (08:00 and 18:00), and each meal was fed at 1.5% of the fish body weight. The amount of feed fed and the amount of residual bait were recorded daily to calculate the actual feed intake of the mandarin fish in each cage. The water quality (dissolved oxygen, pH value, ammonia nitrogen, nitrite, etc.) was tested daily and the number of deaths was recorded. The experimental period was 8 weeks, and samples were taken every 2 weeks to recalculate the weight and feeding amount of the experimental fish in each cage.

[0067] After the culture experiment, the mandarin fish were fasted for 24 h, and six fish were randomly selected from each cage and treated with MS-222 (75 mg L -1 ) After anesthesia, body weight, visceral mass weight, liver weight, mesenteric fat weight and gonad weight were measured. Weight gain rate, survival rate, specific growth rate, feed conversion ratio, intra-abdominal fat rate, visceral index, liver index, survival rate and gonad index were calculated.

[0068] Weight gain rate (WGR, %) = (W2-W1) / W1×100, W1: initial weight, W2: final weight;

[0069] Survival rate (SR, %) = final number of fish / initial number of fish × 100;

[0070] Specific growth rate (SGR, %) = 100 × (lnW2-lnW1) / time (days); W1: initial weight, W2: final weight;

[0071] Feed conversion ratio (FCR, %) = 100 × food intake / weight gain;

[0072] Intraperitoneal fat rate (IPF, %) = 100 × [intraperitoneal fat weight (g) / whole fish weight (g)];

[0073] Viscera to body ratio (VSI, %) = 100 × [viscera weight (g) / whole fish weight (g)];

[0074] Liver-to-body ratio (HSI, %) = 100 × [liver weight (g) / whole fish weight (g)];

[0075] Gonad index (GSI, %) = 100 × [gonad weight (g) / whole fish weight (g)].

[0076] The experimental results are shown in Table 1. The final weight and specific growth rate of the mandarin fish fed with the low fish meal mandarin fish formula feeds of Examples 2 to 4 are significantly higher than those of the mandarin fish fed with the feed of Comparative Example 1; the final weight and specific growth rate of the mandarin fish fed with the low fish meal mandarin fish formula feed of Example 1 are also not significantly different from those of Comparative Example 1. It can be seen that when the fish meal content of Example 1 is only 23% at the minimum, its low fish meal feed will not affect the normal growth of mandarin fish. When the content of fish meal in the formula is appropriately reduced (Examples 2 to 4), the growth of mandarin fish can also be promoted.

[0077] The survival rate and feed conversion rate of the mandarin fish fed with the low-fish meal mandarin fish compound feeds of Examples 1 to 4 were not significantly different from those of the control group, indicating that the reduction of fish meal content in the feed would not affect the feeding and survival of mandarin fish.

[0078] Compared with the comparative example, feeding the low fish meal mandarin fish formula feed of Example 1 significantly increased the viscera to body ratio and liver to body ratio of mandarin fish, while the viscera to body ratio of mandarin fish fed with the low fish meal mandarin fish formula feed of Examples 2 to 4 was not significantly different from that of comparative example 1, and the liver to body ratio of mandarin fish fed with the low fish meal mandarin fish formula feed of Examples 2 to 4 was not significantly different from that of comparative example 1. This indicates that appropriately reducing the fish meal content in the feed has no significant effect on the health of the viscera of mandarin fish.

[0079] The intra-abdominal fat rate and gonad index of the mandarin fish fed with the low-fish meal mandarin fish formula feeds of Examples 2 to 4 were not much different from those of Comparative Example 1, indicating that reducing the fish meal content in the feed formula had no significant effect on the health of the mandarin fish.

[0080] The results of adding a small amount of dephenolized cottonseed protein to the low-fishmeal mandarin fish formula feeds of Examples 1 to 4 show that it does not affect the feeding and growth of mandarin fish, and the feed coefficient does not increase, indicating that mandarin fish has a high tolerance to appropriate and specially treated plant proteins. This breaks the traditional view that mandarin fish is extremely intolerant to plant proteins, and provides important reference significance for the subsequent upgrade of mandarin fish feed formulas.

[0081] Table 1. Test results of breeding experiment

[0082]

[0083] Note: The results are expressed as mean ± standard error (n = 3), and different letters indicate significant differences among the groups (P < 0.05).

[0084] The above is a detailed description of the embodiments of the present invention, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. A low-fishmeal mandarin fish feed, comprising the following ingredients: fish meal, chicken meal, porcine hemoglobin powder, shrimp meal, dephenolized cottonseed protein powder, starch, soybean oil, calcium dihydrogen phosphate, microcrystalline cellulose, premix, rice husk powder and amino acids.

2. The low fish meal mandarin fish compound feed according to claim 1, characterized in that: Calculated by weight, the low-fishmeal mandarin fish feed includes 20-55 parts of fish meal, 15-55 parts of chicken meal, 1-6 parts of porcine hemoglobin powder, 1-4 parts of shrimp meal, 2-8 parts of dephenolized cottonseed protein powder, 8-15 parts of starch, 1-6 parts of soybean oil, 1-3 parts of monocalcium phosphate, 0.5-3 parts of microcrystalline cellulose, 1-4 parts of premix, 0.1-1 parts of rice husk powder and 0.1-1 parts of amino acids.

3. The low fish meal mandarin fish compound feed according to claim 1, characterized in that: Calculated by weight, the low-fishmeal mandarin fish feed includes 23-50 parts of fish meal, 15-50 parts of chicken meal, 1-5 parts of porcine hemoglobin powder, 1-3 parts of shrimp meal, 3-6 parts of dephenolized cottonseed protein powder, 9-13 parts of starch, 1-5 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.5-2.5 parts of microcrystalline cellulose, 1-3 parts of premix, 0.3-1 parts of rice husk powder and 0.1-0.8 parts of amino acids.

4. The low fish meal mandarin fish compound feed according to claim 2, characterized in that: Calculated by weight, the low-fishmeal mandarin fish feed includes 25-50 parts of fish meal, 15-40 parts of chicken meal, 2-4 parts of porcine hemoglobin powder, 2-3 parts of shrimp meal, 4-6 parts of dephenolized cottonseed protein powder, 9-11 parts of starch, 2-5 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.5-2 parts of microcrystalline cellulose, 2-3 parts of premix, 0.5-0.9 parts of rice husk powder and 0.1-0.6 parts of amino acids.

5. The low fish meal mandarin fish compound feed according to any one of claims 1 to 4, characterized in that: The amino acid includes at least one of lysine, methionine and threonine.

6. The low-fishmeal mandarin fish compound feed according to any one of claims 1 to 4, characterized in that: The premix includes vitamins, minerals, sodium chloride, antioxidants and preservatives.

7. The low-fishmeal mandarin fish compound feed according to claim 6, characterized in that: The vitamins include: choline, vitamin A, vitamin D, vitamin E, vitamin K, riboflavin, vitamin B6, pantothenic acid, niacin, biotin, vitamin B12, folic acid, vitamin C; and / or, the minerals include: calcium bicarbonate, potassium chloride, magnesium sulfate, cupric sulfate pentahydrate, ferrous sulfate, zinc sulfate, manganese sulfate, sodium selenate, potassium iodide, sodium molybdate, cobalt sulfate and potassium fluoride.

8. The low fish meal mandarin fish compound feed according to any one of claims 1 to 4, characterized in that: The low-fishmeal mandarin fish compound feed has a protein content of 52% to 56% and a fat content of 10% to 14%.

9. The method for preparing the low-fishmeal mandarin fish compound feed according to any one of claims 1 to 8, comprising the following steps: mixing fish meal, chicken meal, porcine hemoglobin powder, shrimp meal, dephenolized cottonseed protein powder, starch, calcium dihydrogen phosphate, microcrystalline cellulose, premix, rice husk powder and amino acids, conditioning, puffing and granulating, and drying to obtain a semi-finished feed; spraying soybean oil on the surface of the semi-finished feed to obtain the low-fishmeal mandarin fish compound feed.

10. Use of the low-fishmeal mandarin fish compound feed according to any one of claims 1 to 8 in fish farming.