Astragalus extract-containing plant composition with brightening and yellowing-removing effects and preparation method thereof

Abstract

The invention discloses a plant composition containing an astragalus membranaceus extract and having brightening and yellow-removing effects and a preparation method of the plant composition. The composition is prepared from the following raw materials in parts by weight: 20-40 parts of astragalus membranaceus; 10-30 parts of angelica sinensis; 10-30 parts of ligusticum wallichii; 10-20 parts of bighead atractylodes rhizome; and 10-20 parts of rehmannia root. The radix astragali serves as a monarch drug, the radix angelicae sinensis and the rhizoma chuanxiong serve as ministerial drugs, the rhizoma atractylodis macrocephalae and the radix rehmanniae serve as adjuvant drugs, and all the drugs are combined to achieve the beautifying effects of tonifying qi, nourishing blood, activating blood, promoting the circulation of qi, invigorating the spleen, replenishing qi, nourishing yin, promoting the secretion of saliva or body fluid, brightening the skin color and removing yellowness.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a plant composition containing an astragalus extract and having the effects of brightening and removing yellowness, and a preparation method thereof. Background Art

[0002] Yellowish complexion refers to a skin problem caused by various reasons that lead to aging of the skin surface, yellowish complexion and lack of luster. It is the most common physiological aging phenomenon among Asian women. Especially as they age, their faces become more and more "sallow", which seriously affects their appearance and temperament. On the other hand, young women are prone to skin aging due to stress, environmental factors, physical condition, bad living habits, etc., which can also cause yellow complexion.

[0003] The color of human skin is determined by the content of melanin and carotenoids in the epidermis and oxyhemoglobin, hemoglobin, bilirubin and other pigments in the dermis. Melanin is the main influencing factor. By reducing the content of melanin in the epidermis, improving blood circulation in the skin, anti-oxidation and anti-inflammation, it can brighten the skin tone, reduce pigmentation, and improve rough and dark skin. Modern research also shows that the "waxy" face is related to the Maillard reaction. The reducing sugar in the skin cross-links with biological macromolecules such as nucleic acids and proteins to form the insoluble substance lipofuscin, which accumulates in the skin and makes the skin dark yellow.

[0004] According to TCM theory, Qi and blood are the foundation of human beings, and aging is caused by "disharmony of Qi and blood, which leads to all kinds of diseases", that is, Qi deficiency and blood stasis are the root causes of aging. Qi deficiency and blood stasis are mainly caused by poor microcirculation, poor blood flow, and poor metabolism inside the skin. Qi deficiency leads to poor disease resistance, which is manifested as dull complexion, low voice, shortness of breath and fatigue; blood deficiency can cause dull and sallow complexion, dry skin, withered hair and other problems. Therefore, studying TCM theory and TCM from the molecular and cellular levels, and applying TCM theory and other new achievements of modern TCM to modern biological technology, is of great significance to promoting the integration of Chinese and Western medicine and promoting the development of medicine in beauty and skin care. Summary of the invention

[0005] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a plant composition containing astragalus extract with the effect of brightening and removing yellowness and a preparation method thereof. Based on the theory of traditional Chinese medicine, the present invention provides a new formula for replenishing qi and blood with astragalus as the main drug, accurately locates the needs of patients, and improves the problem of dull skin in a targeted manner.

[0006] The objective of the present invention is achieved through the following technical solutions:

[0007] In a first aspect, the present invention provides a plant composition containing an astragalus extract having the effect of brightening and removing yellowness, comprising the following raw materials in parts by weight:

[0008]

[0009] As a preferred embodiment, the raw materials are composed of the following parts by weight:

[0010]

[0011] As a preferred embodiment, the raw materials are composed of the following parts by weight:

[0012]

[0013] In a second aspect, the present invention provides a method for preparing a plant composition containing an astragalus extract having the effect of brightening and removing yellowness, comprising the following steps:

[0014] A. Preparation of Astragalus extract: Add Astragalus to 65-75% ethanol by volume for reflux extraction, concentrate the filtrate, dilute with water and filter, load the concentrated dilution a onto a macroporous resin, then elute with water and 25-35% ethanol by volume, collect the eluate a and concentrate, dilute with water and filter, load the concentrated dilution b onto a macroporous resin, then elute with water and 25-35% ethanol by volume, collect the eluate b and concentrate it to an extract, and obtain an Astragalus extract;

[0015] B. Preparation of Angelica sinensis extract and Chuanxiong extract: Add 75-85% by volume ethanol to Angelica sinensis and Chuanxiong for reflux extraction, concentrate the filtrate, dilute with water, adjust the pH and filter, load the concentrated dilution onto a macroporous resin, and then elute with water and 10-15% by volume ethanol in sequence, discard the eluate, and then elute with 25-35% by volume ethanol, collect the eluate and concentrate it to an extract, to obtain Angelica sinensis extract and Chuanxiong extract;

[0016] C. Preparation of Atractylodes macrocephala extract and Rehmannia glutinosa extract: Add Atractylodes macrocephala and Rehmannia glutinosa into water for reflux extraction, concentrate the obtained filtrate, then add ethanol for alcohol precipitation and filter; dissolve the obtained precipitate with water, then filter with microfiltration membrane and ultrafiltration membrane in sequence, collect the retentate and concentrate it into an extract to obtain Atractylodes macrocephala extract and Rehmannia glutinosa extract;

[0017] D. Preparation of plant extract composition extract: After dissolving Astragalus extract, Angelica extract, Chuanxiong extract, Atractylodes extract and Rehmannia extract with polyol, the mixture is combined and filtered to obtain the filtrate which is the plant composition.

[0018] As a preferred embodiment, in step A, in the reflux extraction, the amount of ethanol added with a volume percentage of 65-75% is 5-15 times the dosage.

[0019] As a preferred embodiment, in step A, the reflux extraction is performed 2 to 3 times, each time for 1.5 to 2.5 hours.

[0020] As a preferred embodiment, in step A, the filtrate is concentrated and then diluted with water to an amount 8 to 12 times the dosage.

[0021] As a preferred embodiment, in step A, the flow rate of the concentrated diluent a and the concentrated diluent b loaded onto the macroporous resin is 0.4-0.6BV / h; the flow rate of water elution is 0.8-1.2BV / h, and the amount of water is 2-4BV of the macroporous resin; the flow rate of elution with ethanol having a volume percentage of 25-35% is 0.8-1.2BV / h, and the amount of 25-35% ethanol is 2-4BV of the macroporous resin.

[0022] As a preferred embodiment, in step A, the eluent a is concentrated and then diluted with water to an amount 2 to 4 times the dosage.

[0023] As a preferred embodiment, in step B, in the reflux extraction, the amount of ethanol added with a volume percentage of 75-85% is 5-15 times the dosage.

[0024] As a preferred embodiment, in step B, the reflux extraction is performed 2 to 3 times, each time for 1.5 to 2.5 hours.

[0025] As a preferred embodiment, in step B, the solution is diluted with water to 8 to 12 times the dosage, and the pH is adjusted to 2.0.

[0026] As a preferred embodiment, in step B, the flow rate of the concentrated dilution liquid loaded onto the macroporous resin is 0.4-0.6BV / h; the flow rate of water elution is 0.8-1.2BV / h, and the amount of water is 2-4BV of the macroporous resin; the flow rate of elution with 10-15% volume percentage ethanol is 0.8-1.2BV / h, and the amount of 10-15% ethanol is 2-4BV of the macroporous resin; the flow rate of elution with 25-35% volume percentage ethanol is 0.8-1.2BV / h, and the amount of 25-35% ethanol is 2-4BV of the macroporous resin.

[0027] As a preferred embodiment, in step C, the amount of water added in the reflux extraction is 5-15 times the amount of the dosage.

[0028] As a preferred embodiment, in step C, the reflux extraction is performed 2 to 3 times, each time for 1.5 to 2.5 hours.

[0029] As a preferred embodiment, in step C, the filtrate is concentrated to a relative density of 1.1 to 1.2, and ethanol is added to an alcohol volume concentration of 65 to 75%; the alcohol precipitation time is 22 to 28 hours.

[0030] As a preferred embodiment, in step C, the precipitate is dissolved in water in an amount 4 to 6 times the dosage; the microfiltration membrane is a 0.2 μm microfiltration membrane; and the ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cutoff of 200 kD.

[0031] As a preferred embodiment, in step B, the angelica and ligusticum chuanxiong can be extracted together, and the obtained extract is the angelica and ligusticum chuanxiong extract.

[0032] As a preferred embodiment, in step C, the Atractylodes macrocephala and Rehmannia root can be extracted together, and the obtained extract is an Atractylodes macrocephala and Rehmannia root extract.

[0033] As a preferred embodiment, in step D, the polyol is selected from at least one of 1,3-butanediol, 1,3-propylene glycol and dipropylene glycol.

[0034] As a preferred solution, in step D, the amount of the polyol used is 1 / 3 to 1 / 5 times the amount of all medicinal materials administered; the volume percentage of the polyol is 40 to 60%.

[0035] In a third aspect, the present invention provides a use of the aforementioned plant composition containing the Astragalus extract having the effect of brightening and removing yellowness or the plant composition prepared according to the aforementioned method in preparing cosmetics.

[0036] In the present invention, the astragalus used is a commonly used clinical Chinese medicine, and its earliest record appears in "Shennong's Herbal Classic": "It tastes sweet and is slightly warm. It is used to treat carbuncle, long-term wound, pus discharge, pain relief, leprosy, five hemorrhoids, rat fistula, tonic, and all kinds of diseases in children." On the one hand, astragalus can nourish the spleen to help the formation of qi and blood, and sufficient qi and blood can make the blood circulate normally in the veins, thereby eliminating blood stasis, removing yellow and brightening; on the other hand, astragalus has the function of raising yang and lifting the sunken, and the head and face are the meeting points of all yang. Using astragalus can raise yang to the head and face, so that qi and blood are prosperous on the face, qi and blood are full to warm and nourish the facial skin, so that blood stasis can be eliminated, and the meridians are unblocked, thereby eliminating the dark yellow of the skin, brightening the skin color, making the skin ruddy and shiny, and the skin texture is delicate. Modern pharmacological studies have shown that the effective ingredients of astragalus are mainly astragalus total flavonoids, astragalus total saponins, astragalus polysaccharides, etc.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] The present invention is based on the theory of traditional Chinese medicine and combines a variety of Chinese medicinal plants to make the formula have the effect of removing jaundice. The astragalus used in the present invention has the effect of replenishing qi and raising yang, and is mainly used for qi deficiency symptoms, plays a role in improving overall qi and blood, and enhances the qi and blood circulation of the skin, thereby improving dull skin color and achieving the effect of brightening the skin color. It is the main medicine; the angelica used has the effect of replenishing blood and activating blood circulation, and is mainly used for blood deficiency and blood stasis. Angelica combined with astragalus can further improve the problem of insufficient qi and blood, promote blood circulation, moisturize the skin, and reduce facial yellowness. It is the ministerial medicine; the Chuanxiong used has the effect of activating blood circulation and qi, dispelling wind and relieving pain, and it can help Angelica can better promote blood circulation, and promote qi circulation to dredge meridians, speed up blood circulation, and help remove facial yellowness. It is a ministerial drug together with Angelica; the Atractylodes macrocephala used has the effect of strengthening the spleen and replenishing qi, and it can assist Astragalus membranaceus to strengthen the qi and blood tonic effect, and is an adjuvant drug; the Rehmannia root used has the effect of nourishing yin and promoting fluid, cooling blood and stopping bleeding, and can help moisturize the skin and improve dry skin problems. It is an adjuvant drug together with Atractylodes macrocephala; all the medicines are used together to achieve the beauty effects of replenishing qi and blood, promoting blood circulation and promoting qi circulation, strengthening the spleen and replenishing qi, and nourishing yin and promoting fluid to achieve brightening skin tone and removing yellowness.

[0039] The plant composition prepared by the present invention has a synergistic effect of each component, and it has been verified that its AGEs inhibition rate can reach 60.69-67.48%, the melanin synthesis inhibition rate can reach 51.58-55.47%, and the tyrosinase activity inhibition rate can reach 53.75-56.40%, and has very significant anti-glycation effect and the effect of inhibiting melanin synthesis and inhibiting tyrosinase activity; it has a good scavenging effect on ABTS free radicals between the concentration of 0.0313-0.5% (V / V), so it has a good antioxidant effect. The human efficacy test results show that the plant composition prepared by the present invention can reduce the change rate of skin MI value by 5.67-6.94%, increase the change rate of skin L* value by 5.19-5.78%, and increase the change rate of skin ITA° value by 10.58-12.31% after being prepared into a face cream for 4 weeks. It has a very good effect of reducing skin melanin content and effectively improving skin brightness and skin dullness.

[0040] Instruction Manual

[0041] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0042] Figure 1 The results of scavenging ABTS free radicals by test solutions of different concentrations are shown in Figure 2. DETAILED DESCRIPTION

[0043] The present invention is described in detail below in conjunction with specific embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but are not intended to limit the present invention in any form. It should be noted that, for those of ordinary skill in the art, several variations and improvements may be made without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0044] The words "preferred", "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects in certain circumstances. However, other embodiments may also be preferred under the same circumstances or other circumstances. In addition, the description of one or more preferred embodiments does not imply that other embodiments are not applicable, nor is it intended to exclude other embodiments from the scope of the present invention.

[0045] It should be understood that, except in any operating examples, or otherwise indicated, all numbers representing the amount of ingredients used in the specification and claims should be understood to be modified by the term "about" in all cases. Therefore, unless otherwise indicated, the numerical parameters set forth in the following specification and the appended claims are approximate values ​​that vary according to the desired performance to be obtained by the present invention. At least, it is not intended to limit the application of the doctrine of equivalents to the scope of the claims, and each numerical parameter should at least be interpreted according to the number of reported significant figures and by applying ordinary rounding techniques.

[0046] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values ​​set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

[0047] In a specific embodiment of the present invention, a plant composition containing an astragalus extract having the effect of brightening and removing yellowing is provided, comprising the following raw materials in parts by weight:

[0048] 20-40 parts of Astragalus;

[0049] 10-30 portions of Chinese Angelica;

[0050] 10-30 parts of Chuanxiong;

[0051] 10-20 portions of Atractylodes macrocephala;

[0052] 10-20 portions of Rehmannia root.

[0053] In a specific embodiment, the present invention also provides a method for preparing the plant composition containing the astragalus extract having the effect of brightening and removing yellowness, comprising the following steps:

[0054] A. Preparation of Astragalus extract: Add 5-15 times the volume of 65-75% ethanol to Astragalus for reflux extraction 2-3 times, each time for 1.5-2.5 hours, concentrate the filtrate, then dilute with water to 8-12 times the dosage and filter, and load the concentrated dilution a onto a macroporous resin at a flow rate of 0.4-0.6 BV / h, then add water at a flow rate of 0.8-1.2 BV / h (the amount used is 2-4 BV of the macroporous resin), 25-35% ethanol at a flow rate of 0.8-1.2 BV / h, and (the dosage is 2-4 BV of the macroporous resin), the eluate a is collected and concentrated, then diluted with water to 2-4 times the dosage and filtered, the concentrated dilution b is then loaded onto the macroporous resin at a flow rate of 0.4-0.6 BV / h, and then eluted with water at a flow rate of 0.8-1.2 BV / h (the dosage is 2-4 BV of the macroporous resin), and ethanol with a volume percentage of 25-35% at a flow rate of 0.8-1.2 BV / h (the dosage is 2-4 BV of the macroporous resin), the eluate b is collected and concentrated to an extract, and an Astragalus extract is obtained;

[0055] B. Preparation of Angelica extract and Chuanxiong extract: Add 5 to 15 times the volume of 75 to 85% ethanol to Angelica and Chuanxiong for reflux extraction 2 to 3 times, each time for 1.5 to 2.5 hours. The obtained filtrate is concentrated, then diluted with water to 8 to 12 times the dosage, adjusted to pH 2.0 and filtered. The obtained concentrated dilution is loaded onto a macroporous resin at a flow rate of 0.4 to 0.6 BV / h, and then successively filtered with a flow rate of 0.8 to 1.2 BV / h. / h of water (the amount used is 2-4 BV of the macroporous resin), eluted with 10-15% ethanol (the amount used is 2-4 BV of the macroporous resin) at a flow rate of 0.8-1.2 BV / h, discarded the eluate, and then eluted with 25-35% ethanol (the amount used is 2-4 BV of the macroporous resin) at a flow rate of 0.8-1.2 BV / h, collected the eluate and concentrated it to an extract, to obtain angelica extract and ligusticum chuanxiong extract;

[0056] C. Preparation of Atractylodes macrocephala extract and Rehmannia glutinosa extract: Add 5-15 times the amount of water to Atractylodes macrocephala and Rehmannia glutinosa for reflux extraction 2-3 times, each time for 1.5-2.5 hours, and concentrate the obtained filtrate to a relative density of 1.1-1.2, then add ethanol to an alcohol volume concentration of 65-75%, perform alcohol precipitation for 22-28 hours, and then filter; the obtained precipitate is dissolved with 4-6 times the amount of water, and then filtered with a 0.2μm microfiltration membrane and a 200kD ultrafiltration membrane with a molecular weight cutoff, collect the retentate and concentrate it to an extract, to obtain the Atractylodes macrocephala extract and Rehmannia glutinosa extract;

[0057] D. Preparation of plant extract composition extract: Dissolve astragalus extract, angelica extract, ligusticum chuanxiong extract, atractylodes extract and rehmannia extract with 1 / 3 to 1 / 5 times the amount of polyol, combine and filter, and the obtained filtrate is the plant composition.

[0058] The plant composition of the present invention can be prepared by the above methods.

[0059] In the following examples, all raw materials used are commercially available raw materials, and the present invention does not make any particular limitation.

[0060] Example 1

[0061] This embodiment provides a plant composition containing an astragalus extract having the effect of brightening and removing yellowing, and the composition and weight proportion of each raw material thereof are shown in Table 1.

[0062] The preparation steps of the plant composition are as follows:

[0063] S10, preparation of astragalus extract: weigh 30g of astragalus slices, add 10 times (300mL) of 70% ethanol, reflux and extract twice, each time for 2h; combine the two filtrates, concentrate until there is no alcohol taste, dilute with purified water to 10 times (300mL) of the dosage, and filter; take the treated macroporous resin (resin model is NKA-9, the same weight as the astragalus slices, 30g), fill it into the chromatography column, load the concentrated dilution a at a flow rate of 0.5BV / h, and elute with 3BV of purified water at a flow rate of 1.0BV / h, The eluent was discarded; 3BV of 30% ethanol was used for elution at a flow rate of 1.0BV / h, the eluent was collected and concentrated until there was no alcohol taste, and it was diluted with purified water to 3 times the dosage (90mL), and filtered; the concentrated dilution b was passed through a macroporous resin column (1 / 2 of the weight of the Astragalus slices, 15g) again, loaded at a flow rate of 0.5BV / h, and eluted with 3BV of purified water at a flow rate of 1.0BV / h, and the eluent was discarded; 3BV of 30% ethanol was used for elution at a flow rate of 1.0BV / h, and the eluent was collected and concentrated to an extract;

[0064] S20, preparation of extracts of angelica and chuanxiong: weigh 25g of angelica slices and 20g of chuanxiong slices, add 10 times (450mL) of 80% ethanol, reflux and extract twice, each time for 2h; combine the two filtrates, concentrate until there is no alcohol taste, dilute with purified water to 10 times (450mL) of the dosage, adjust the pH to 2.0, and filter; take the treated macroporous resin (resin model DA201, equal weight with angelica and chuanxiong, 45g), fill it into the chromatography column, load the concentrated dilution at a flow rate of 0.5BV / h, elute with 3BV of purified water and 3BV of 10% ethanol at a flow rate of 1.0BV / h, respectively, and discard the eluate; then elute with 3BV of 30% ethanol at a flow rate of 1.0BV / h, collect the eluate and concentrate it to an extract;

[0065] S30, preparation of extract of Atractylodes macrocephala and Rehmannia root: weigh 15g of Atractylodes macrocephala slices and 10g of Rehmannia root slices, add 10 times (250mL) of water, reflux and extract twice, each time for 2h; combine the two filtrates, concentrate to a relative density of 1.15, add ethanol to an alcohol concentration of 70%, precipitate with alcohol, let stand for 24h, and filter; dissolve the precipitate with purified water to 5 times (125mL) of the dosage, filter with a microfiltration membrane of 0.2μm and an ultrafiltration membrane with a molecular weight cutoff of 200kD in sequence, collect the retentate and concentrate it to an extract;

[0066] S40, preparation of plant extract composition extract: use 50% 1,3-butanediol with 1 / 4 weight (25mL) of all medicinal materials to dissolve the extracts of S10, S20 and S30, combine and filter to obtain a filtrate, which contains a plant extract composition extract with brightening and yellowing removal effect, and the obtained extract has a total flavonoid content of 0.30%, a total organic acid content of 1.8%, and a total polysaccharide content of 1.2%.

[0067] Example 2

[0068] This embodiment provides a plant composition containing an astragalus extract having the effect of brightening and removing yellowing, and the composition and weight proportion of each raw material thereof are shown in Table 1.

[0069] The preparation steps of the plant composition are the same as those in Example 1. The obtained extract has a total flavonoid content of 0.41%, a total organic acid content of 1.6%, and a total polysaccharide content of 0.89%.

[0070] Example 3

[0071] This embodiment provides a plant composition containing an astragalus extract having the effect of brightening and removing yellowing, and the composition and weight proportion of each raw material thereof are shown in Table 1.

[0072] The preparation steps of the plant composition are the same as those in Example 1. The extract obtained has a total flavonoid content of 0.19%, a total organic acid content of 1.6%, and a total polysaccharide content of 1.9%.

[0073] Comparative Example 1

[0074] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are as follows:

[0075] Weigh 100g of Astragalus slices, add 10 times the amount of 70% ethanol, reflux and extract twice, each time for 2 hours; combine the two filtrates, concentrate until there is no alcohol taste, dilute with purified water to 10 times the amount of the dosage, and filter; take 100g of the treated macroporous resin, fill it into the chromatography column, load the concentrated dilution a at a flow rate of 0.5BV / h, elute with 3BV of purified water at a flow rate of 1.0BV / h, and discard the eluent; then use 3BV of 30% ethanol was eluted at a flow rate of 1.0 BV / h, the eluate was collected and concentrated until there was no alcohol taste, diluted with purified water to 3 times the dosage, and filtered; the concentrated dilution b was passed through a 50g macroporous resin column again, loaded at a flow rate of 0.5 BV / h, eluted with 3 BV of purified water at a flow rate of 1.0 BV / h, and the eluate was discarded; 3 BV of 30% ethanol was eluted at a flow rate of 1.0 BV / h, the eluate was collected and concentrated to an extract;

[0076] The extract was taken, dissolved in 25 mL of 50% 1,3-butanediol, and filtered to obtain a filtrate. The total flavonoid content of the obtained extract was 0.29%.

[0077] Comparative Example 2

[0078] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those of Example 3.

[0079] Comparative Example 3

[0080] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those of Example 3.

[0081] Comparative Example 4

[0082] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those of Example 3.

[0083] Comparative Example 5

[0084] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those of Example 3.

[0085] Comparative Example 6

[0086] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are basically the same as those in Example 3, except that the decoction pieces of Rehmannia root in step S30 are replaced with decoction pieces of Rhubarb to prepare Atractylodes macrocephala and Rhubarb extract.

[0087] Comparative Example 7

[0088] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those in Example 1.

[0089] Comparative Example 8

[0090] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those in Example 1.

[0091] Comparative Example 9

[0092] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those in Example 1.

[0093] Comparative Example 10

[0094] This comparative example provides a plant composition containing astragalus extract with the effect of brightening and removing yellowness, and the composition and weight proportion of each raw material are shown in Table 1. The preparation steps of the plant composition are the same as those in Example 1.

[0095] Table 1

[0096]

[0097]

[0098] Comparative Example 11

[0099] 0.25 g of commercially available 70% astragalus polysaccharide (purchased from Shanghai Yuanshi Technology Co., Ltd.) was dissolved in 25 mL of 50% 1,3-butanediol and filtered to obtain a filtrate.

[0100] Comparative Example 12

[0101] 0.25 g of commercially available 10% astragaloside IV (purchased from Shanghai Yuanshi Technology Co., Ltd.) was dissolved in 25 mL of 50% 1,3-butanediol and filtered to obtain a filtrate.

[0102] Efficacy verification test

[0103] Test Example 1: Experiment on the ability of plant extract composition to inhibit AGEs production

[0104] (1) Solution preparation

[0105] Preparation of sample solution: dilute each composition prepared in Examples 1-3 and Comparative Examples 1-12 by 20 times with PBS solution (pH 7.4).

[0106] Glycosylation reaction solution: 4 g bovine serum albumin, 12 g D-(+)-glucose, 100 mL PBS solution, mix and dissolve to obtain.

[0107] (2) Experimental operation

[0108] Table 2

[0109]

[0110] The samples were grouped according to Table 2, and the reagents were added in sequence. PBS solution was used instead of the sample as the blank group. After mixing evenly, the mixture was incubated at 55°C for 4 days. After the reaction, the incubated solution was cooled to room temperature, filtered through a 0.22 μm filter membrane, and 200 μL of the reaction solution was added to a 96-well plate in sequence. The content of the final product fluorescent substance AGEs was detected using a fluorescent microplate reader under the conditions of an excitation wavelength of 320 nm and an emission wavelength of 460 nm, and the AGEs inhibition rate was calculated according to the following formula.

[0111]

[0112] Where:

[0113] A- Fluorescence intensity of the saccharification system of the sample group

[0114] B- Fluorescence intensity of PBS solution in sample group

[0115] C- Fluorescence intensity of the saccharification system in the blank group

[0116] D- Fluorescence intensity of PBS solution in blank group

[0117] (3) The test results are shown in Table 3.

[0118] Table 3

[0119] Test samples AGEs inhibition rate (%) Blank Group / Example 1 67.48±1.94 Example 2 65.12±2.72 Example 3 60.69±1.89 Comparative Example 1 39.77±2.33 Comparative Example 2 45.15±3.49 Comparative Example 3 43.79±2.67 Comparative Example 4 48.10±3.16 Comparative Example 5 49.77±2.97 Comparative Example 6 42.06±3.21 Comparative Example 7 51.42±3.34 Comparative Example 8 53.15±2.99 Comparative Example 9 50.83±2.46 Comparative Example 10 49.54±2.25 Comparative Example 11 29.07±2.10 Comparative Example 12 32.87±2.49

[0120] From Table 3, it can be found that the activity of the sample in comparative example 1 in inhibiting AGEs is better than that in comparative examples 11 and 12, indicating that the anti-glycation effect of total flavonoids of Astragalus is better than that of Astragalus polysaccharides and Astragalus saponins. The activity of the plant extract composition prepared in Examples 1-3 in inhibiting AGEs is better than that in comparative examples 1-10, indicating that the compounding of Astragalus with other specific plant components (Angelica sinensis, Chuanxiong, Atractylodes macrocephala, Rehmannia glutinosa) effectively improves its anti-glycation effect.

[0121] Test Example 2: B16 cell efficacy evaluation experiment of plant extract composition

[0122] (1) Solution preparation

[0123] Sample preparation: Dissolve each composition prepared in Examples 1-3 and Comparative Examples 1-12 in PBS solution to a concentration of 0.2%.

[0124] (2) Cell culture

[0125] B16 cells were cultured in RPMI-1640 complete medium (containing 10% fetal bovine serum) at 37°C and 5% CO 2 (CO in the air of the incubator 2 The cells were cultured under the condition of 5% volume fraction.

[0126] (3) Evaluation test of the effect of inhibiting tyrosinase activity

[0127] B16 cells in the exponential growth phase (purchased from Shangcheng Beina Chuanglian Biotechnology Co., Ltd.) were digested and dispersed to prepare a single cell suspension, and the cell density was adjusted to 1×10 per well. 4 The cells were inoculated in a 96-well plate at a density of 100 cells / well and cultured for 12 hours. When the cells were completely attached to the wall, the supernatant was discarded. Each test sample (as a test group) was added and 3 replicate wells were set up. After 48 hours of culture, the supernatant was discarded. After washing twice with PBS solution, 50 μL of PBS solution containing 0.5% TritonX-100 was added to each well and quickly placed in a -80°C low-temperature refrigerator for 2 hours. Take it out and place it at room temperature to melt; at the same time, a blank group (50 μL of PBS solution containing 0.5% TritonX-100) was set up. After incubation of each group, 100 μL of L-DOPA with a mass concentration of 10 mmol / L was added, reacted at 37°C for 2 hours, and the absorbance value at 475 nm was measured on an enzyme reader. The enzyme activity was calculated according to the following formula: Tyrosinase activity inhibition rate = (OD value of the control group - OD value of the test group) / (OD value of the control group - OD value of the blank group) × 100%.

[0128] (4) Inhibition of melanin synthesis in melanocytes

[0129] Take B16 cells in the exponential growth phase and adjust the cell density to 4×10 per well. 5Inoculate in 6-well plates, discard the supernatant after 12 hours, add PBS solution containing each test sample (as the test group) or an equal volume of PBS solution (as the blank group), and set up 3 replicate wells. After 48 hours of culture, wash twice with PBS solution, digest and collect cells in a centrifuge tube, centrifuge at 1500r / min for 10 minutes, and discard the supernatant. Add 2mL of PBS solution, gently blow with a pipette to make a cell suspension (of which 20μL is diluted a certain multiple for cell counting), add 500μL of ethanol and ether mixture (volume ratio 1:1), leave at room temperature for 30min, centrifuge at 3000r / min for 5min, discard the supernatant, add 1mL of 1mol / L NaOH solution containing 10% DMSO, mix carefully, incubate in 80℃ water bath for 2h, and measure the absorbance value at 405nm. Melanin synthesis inhibition rate = (OD value of test group / corresponding cell number) / (OD value of blank group / corresponding cell number)×100%. The results are shown in Table 4.

[0130] Table 4

[0131] Test samples Melanin synthesis inhibition rate (%) Tyrosinase activity inhibition rate (%) Blank Group / / Example 1 55.47±1.31 56.40±4.57 Example 2 53.36±3.43 54.63±1.56 Example 3 51.58±4.24 53.75±2.33 Comparative Example 1 25.72±1.60 37.65±1.45 Comparative Example 2 38.27±4.18 39.53±3.77 Comparative Example 3 36.51±2.56 37.43±3.36 Comparative Example 4 39.14±4.01 40.78±4.39 Comparative Example 5 40.22±3.25 43.15±3.13 Comparative Example 6 33.75±2.95 35.28±2.94 Comparative Example 7 42.20±3.54 44.32±4.61 Comparative Example 8 45.28±3.47 47.69±3.05 Comparative Example 9 43.97±2.28 44.72±3.66 Comparative Example 10 41.64±4.64 42.89±3.25 Comparative Example 11 12.59±2.81 28.82±2.61 Comparative Example 12 7.36±3.67 22.86±1.88

[0132] From Table 4, it can be found that the sample of Comparative Example 1 has better inhibitory effects on melanin synthesis and tyrosinase than Comparative Examples 11 and 12, indicating that the total flavonoids of Astragalus are better than Astragalus polysaccharides and Astragalus saponins. The plant extract compositions prepared in Examples 1-3 have better inhibitory effects on melanin synthesis and tyrosinase than Comparative Examples 1-10, indicating that the compounding of Astragalus with other specific plant formulas (Angelica sinensis, Chuanxiong, Atractylodes macrocephala, Rehmannia glutinosa) effectively improves its efficacy in inhibiting melanin synthesis and tyrosinase activity.

[0133] Test Example 3: Experiment on scavenging of ABTS free radicals by plant extract composition

[0134] (1) Solution preparation

[0135] Preparation of test solution: The composition prepared in Example 1 was diluted with PBS solution to a series of gradient concentrations, namely 0.5%, 0.25%, 0.125%, 0.0625%, 0.0313%

[0136] Preparation of ABTS working solution: ABTS was prepared by mixing equal volumes of ABTS solution (7.4 mmol / L) and potassium persulfate aqueous solution (2.6 mmol / L) and incubating the mixture at room temperature in the dark overnight. + The absorbance of the mixture was adjusted to 0.7±0.02 at 734 nm using PBS solution.

[0137] (2) Experimental operation

[0138] Table 5

[0139]

[0140] The samples were grouped according to Table 5, and the reagents were added and mixed in sequence. The absorbance value was measured at a wavelength of 734 nm using an ELISA reader, and the scavenging rate of the test sample on ABTS free radicals was calculated according to the following formula.

[0141]

[0142] Where:

[0143] T 1 ——The sample group has the absorbance value of the ABTS system

[0144] T 2 ——The sample group does not have the absorbance value of the ABTS system

[0145] C 1 ——The blank group has the absorbance value of the ABTS system

[0146] C 2 ——Blank group has no ABTS system absorbance value

[0147] (3) The test results are shown in Table 6 and Figure 1 .

[0148] Table 6 Results of scavenging ABTS free radicals by different concentrations of test solution

[0149] Concentration of test solution (%) ABTS free radical scavenging rate (%) 0.0313 13.27 0.0625 24.34 0.125 41.73 0.25 74.45 0.5 99.73

[0150] As shown in Table 6, the plant extract composition provided by the present invention has a good scavenging effect on ABTS free radicals at a concentration of 0.0313-0.5% (V / V), and the scavenging ability increases with the increase of concentration, indicating that the plant extract composition provided by the present invention has a good antioxidant effect.

[0151] Test Example 4: Evaluation experiment on the effect of plant extract composition on brightening and removing yellowness in human body

[0152] Facial creams rich in the plant extract compositions prepared in Examples 1-3 and Comparative Examples 1-6 were prepared respectively. The specific raw material compositions and percentage ratios of the facial creams are shown in Table 7, and a facial cream prepared from a blank matrix without adding the compositions prepared in Examples 1-3 and Comparative Examples 1-6 was used as a blank control.

[0153] Table 7

[0154] Raw material composition Mass percentage (%) Composition of plant extracts 5 Squalane 14.5 GTCC 4 MONTANOV 68MB 1.2 Sorbitan Stearate 1.6 NIKKOMULESE 41 1 water 62.15 EDTA·2Na 0.05 D-Panthenol 0.5 Trehalose 3 glycerin 5 ARISTOFLEX AVC 0.8 Phenoxyethanol 1 essence 0.2

[0155] Selection and grouping of subjects:

[0156] 100 females, aged between 30 and 60 years old (except pregnant or lactating women); no serious systemic diseases, immunodeficiency or autoimmune diseases; no skin treatment, cosmetic treatment or other tests that may affect the results at the test site; no active allergic diseases; no highly sensitive constitution; no use of hormonal drugs and immunosuppressants in the past month; and no other clinical trials at the test site in the past month.

[0157] The subjects were randomly divided into 10 groups: a facial cream group containing plant extract composition and a blank facial cream group, with 10 subjects in each group. Test method and product use:

[0158] Before the test, the subjects cleaned their faces uniformly and dried them with a dandruff-free absorbent paper towel. After sitting quietly for 30 minutes in a constant temperature and humidity environment ((21±1)℃, relative humidity (50±10)%), the technicians operated the relevant instruments to collect basic data on the subjects' faces, including skin melanin content (MI value), skin whiteness (L* value) and skin dark yellowness (ITA° value).

[0159] After the above skin parameter tests are completed, the samples are distributed to the subjects according to the groups, and the subjects use them according to the product instructions. The experiment adopts a half-face test, that is, after the subjects wash their faces and dry them every morning and evening, they use a cream rich in the present invention (the plant extract composition prepared by Examples 1-3 and Comparative Examples 1-6) on the right face once, and a blank cream on the left face as a control. The amount of application on both sides is equal each time, and the application is even, and it is used continuously for 4 weeks. During the trial, it is not used with other skin care products with whitening or brightening effects. The subjects are required to follow up at the same time during the day each time, and to return visits at the set test time points (2 weeks and 4 weeks after using the product), and the test process is consistent with the basic data collection.

[0160] Instrument test:

[0161] At each visit point, the melanin content (MI value) of the skin before and after the use of the sample was measured using the Mexameter MX18 skin melanin and hemoglobin tester; the whiteness (L* value) and dull yellowness (ITA° value) of the skin before and after the use of the sample were measured using the skin color test probe CL-400. The test results are shown in Tables 8-10.

[0162] Table 8 Skin MI value test results ( ±s, n=10)

[0163]

[0164] Note: Compared with the baseline value, ***P<0.001, **P<0.01, *P<0.05

[0165] Table 9 Skin L* value test results ( n=10)

[0166]

[0167]

[0168] Note: Compared with the baseline value, ***P<0.001, **P<0.01, *P<0.05

[0169] Table 10 Skin ITA° value test results ( n=10)

[0170]

[0171] Note: Compared with the baseline value, ***P<0.001, **P<0.01, *P<0.05

[0172] From Table 8, it can be found that after the subjects used the creams rich in Examples 1-3, the skin MI values ​​were lower than before use during the test period. Compared with the basal value, the skin MI values ​​of the subjects were significantly reduced after using the product for 2 weeks and 4 weeks (P < 0.01), and the test results were significantly better than the creams of Comparative Examples 1-6; after using the blank cream, the skin MI values ​​did not change much compared with the corresponding basal values ​​during the test period, and there was no significant difference (P > 0.05). It can be seen that the cream rich in the present invention can effectively reduce the melanin content of the skin.

[0173] From Table 9, it can be found that after the subjects used the creams enriched with Examples 1-3, the skin L* value gradually increased during the test period. Compared with the basal value, the skin L* value of the subjects increased significantly after using the product for 2 weeks and 4 weeks (P < 0.05), and the test results were significantly better than the creams of Comparative Examples 1-6; after using the blank cream, the skin L* value did not change much compared with the corresponding basal value during the test period, and there was no significant difference (P > 0.05). It can be seen that the cream enriched with the present invention can effectively improve skin brightness.

[0174] It can be found from Table 10 that after the subjects used the creams enriched with Examples 1-3, the skin ITA° value gradually increased during the test period. Compared with the basal value, the skin ITA° value of the subjects increased significantly after using the product for 2 weeks and 4 weeks (P < 0.05), and the test results were significantly better than the creams of Comparative Examples 1-6; after using the blank cream, the skin ITA° value did not change much compared with the corresponding basal value during the test period, and there was no significant difference (P > 0.05). It can be seen that the cream enriched with the present invention can effectively improve the dullness of the skin.

[0175] The above experimental results show that the combination of Astragalus and other specific plant formulas (Angelica sinensis, Chuanxiong, Atractylodes macrocephala, Rehmannia glutinosa) effectively improves its brightening and yellowing removal effects. The plant extract composition containing Astragalus extract prepared by the present invention can be used in cosmetics for brightening and yellowing removal.

[0176] The present invention has many specific application paths, and the above is only a preferred embodiment of the present invention. It should be pointed out that the above embodiments are only used to illustrate the present invention, and are not used to limit the protection scope of the present invention. For those of ordinary skill in the art, several improvements can be made without departing from the principle of the present invention, and these improvements should also be regarded as the protection scope of the present invention.

Claims

1. A plant composition containing astragalus extract having the effect of brightening and removing yellowness, characterized in that: The composition includes the following raw materials in parts by weight:

2. The plant composition containing astragalus extract with the effect of brightening and removing yellowness according to claim 1, characterized in that: The composition includes the following raw materials in parts by weight:

3. The plant composition containing astragalus extract with the effect of brightening and removing yellowness according to claim 1, characterized in that: The composition includes the following raw materials in parts by weight:

4. A method for preparing a plant composition containing an astragalus extract having the effect of brightening and removing yellowing according to any one of claims 1 to 3, characterized in that: The following steps are involved: A. Preparation of Astragalus extract: Add Astragalus to 65-75% ethanol by volume for reflux extraction, concentrate the filtrate, dilute with water and filter, load the concentrated dilution a onto a macroporous resin, then elute with water and 25-35% ethanol by volume in sequence, collect the eluate a and concentrate, then dilute with water and filter, load the concentrated dilution b onto a macroporous resin, then elute with water and 25-35% ethanol by volume in sequence, collect the eluate b and concentrate it to an extract, and obtain an Astragalus extract; B. Preparation of Angelica sinensis extract and Chuanxiong extract: Add 75-85% by volume ethanol to Angelica sinensis and Chuanxiong for reflux extraction, concentrate the filtrate, dilute with water, adjust the pH and filter, load the concentrated dilution onto a macroporous resin, and then elute with water and 10-15% by volume ethanol in sequence, discard the eluate, and then elute with 25-35% by volume ethanol, collect the eluate and concentrate it to an extract, to obtain Angelica sinensis extract and Chuanxiong extract; C. Preparation of Atractylodes macrocephala extract and Rehmannia glutinosa extract: Add Atractylodes macrocephala and Rehmannia glutinosa into water for reflux extraction, concentrate the obtained filtrate, then add ethanol for alcohol precipitation and filter; dissolve the obtained precipitate with water, then filter with microfiltration membrane and ultrafiltration membrane in sequence, collect the retentate and concentrate it into an extract to obtain Atractylodes macrocephala extract and Rehmannia glutinosa extract; D. Preparation of plant extract composition extract: After dissolving Astragalus extract, Angelica extract, Chuanxiong extract, Atractylodes extract and Rehmannia extract with polyol, the mixture is combined and filtered to obtain the filtrate which is the plant composition.

5. The method for preparing the plant composition containing astragalus extract having the effect of brightening and removing yellowing according to claim 4, characterized in that: In step A, in the reflux extraction, the amount of ethanol added with a volume percentage of 65-75% is 5-15 times the amount of the dosage; The reflux extraction is performed 2 to 3 times, each time for 1.5 to 2.5 hours; The filtrate is concentrated and diluted with water to 8 to 12 times the dosage; The flow rate of the concentrated diluent a and the concentrated diluent b loaded onto the macroporous resin is 0.4-0.6 BV / h; the flow rate of the water elution is 0.8-1.2 BV / h, and the amount of water is 2-4 BV of the macroporous resin; the flow rate of the elution with 25-35% volume percentage ethanol is 0.8-1.2 BV / h, and the amount of 25-35% volume percentage ethanol is 2-4 BV of the macroporous resin; The eluent a is concentrated and diluted with water to an amount 2 to 4 times the dosage.

6. The method for preparing the plant composition containing astragalus extract having the effect of brightening and removing yellowing according to claim 4, characterized in that: In step B, in the reflux extraction, the amount of ethanol added with a volume percentage of 75-85% is 5-15 times the amount of the dosage; The reflux extraction is performed 2 to 3 times, each time for 1.5 to 2.5 hours; The dilution with water is 8 to 12 times the dosage, and the pH is adjusted to 2.0; The flow rate of the concentrated dilution liquid loaded onto the macroporous resin is 0.4-0.6BV / h; the flow rate of water elution is 0.8-1.2BV / h, and the amount of water used is 2-4BV of the macroporous resin; the flow rate of elution with 10-15% volume percentage ethanol is 0.8-1.2BV / h, and the amount of 10-15% volume percentage ethanol is 2-4BV of the macroporous resin; the flow rate of elution with 25-35% volume percentage ethanol is 0.8-1.2BV / h, and the amount of 25-35% volume percentage ethanol is 2-4BV of the macroporous resin.

7. The method for preparing the plant composition containing astragalus extract having the effect of brightening and removing yellowing according to claim 4, characterized in that: In step C, in the reflux extraction, the amount of water added is 5 to 15 times the amount of the drug; The reflux extraction is performed 2 to 3 times, each time for 1.5 to 2.5 hours; The filtrate is concentrated to a relative density of 1.1 to 1.2, and ethanol is added to an alcohol volume concentration of 65 to 75%; the alcohol precipitation time is 22 to 28 hours; The precipitate is dissolved with water in an amount 4 to 6 times the dosage; the microfiltration membrane is a 0.2 μm microfiltration membrane; and the ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cutoff of 200 kD.

8. The method for preparing the plant composition containing astragalus extract with the effect of brightening and removing yellowness according to claim 4, 6 or 7, characterized in that: In step B, the angelica and chuanxiong can be extracted together, and the obtained extract is the angelica and chuanxiong extract; In step C, the Atractylodes macrocephala and Rehmannia root can be extracted together, and the obtained extract is the Atractylodes macrocephala and Rehmannia root extract.

9. The method for preparing the plant composition containing astragalus extract having the effect of brightening and removing yellowing according to claim 4, characterized in that: In step D, the polyol is selected from at least one of 1,3-butanediol, 1,3-propylene glycol, and dipropylene glycol; The dosage of the polyol is 1 / 3 to 1 / 5 times of the dosage of all medicinal materials; the volume percentage of the polyol is 40 to 60%.

10. Use of the plant composition containing the astragalus extract having the effect of brightening and removing yellowness according to any one of claims 1 to 3 or the plant composition prepared by the method according to any one of claims 4 to 9 in preparing cosmetics.

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