Cryogenic preservation device with integrated tracking chamber

Abstract

This invention provides a cryopreservation device with an integrated tracking device chamber. The tracking device can be an RFID tag, which can be placed in the cryopreservation device as part of the vitrification process of the biological sample. The cryopreservation device maintains its integrity under the environmental conditions at sample collection and under liquid nitrogen conditions during sample storage, and securely holds the RFID tag within the chamber. The cryopreservation device with an integrated tracking device chamber allows each individual biological sample to have its own tracking device for monitoring during cryopreservation.

Description

[0001] This application claims the benefit of U.S. non-provisional application No. 18 / 136,419, filed April 19, 2023, which is incorporated herein by reference in its entirety. Technical Field

[0002] This invention generally relates to the field of devices for the cryopreservation of biological samples. More specifically, this invention relates to devices capable of remote identification via RFID technology. Background Technology

[0003] In life sciences, cryopreservation is performed to cease the biological activity of living cells over an extended period of time. Vitrification is one such technique used for cryopreservation.

[0004] Vitrification involves transforming a solution containing a biological sample (i.e., an oocyte or embryo) into a glassy, ​​amorphous solid without any crystalline structure, followed by rapid cooling. One of the main challenges of this method is preventing the formation of ice crystals from the intracellular fluid within the oocyte or embryo. Therefore, the first step is to dehydrate one or more cells as much as possible using a fluid containing a cryoprotectant, known as a “vitrification medium.” The biological sample is then rapidly frozen by immersion in a cryogenic fluid such as liquid nitrogen (LN2). Due to the appropriate combination of freezing rate and cryoprotectant concentration, the water within the cells will reach a solid, harmless, glassy (vitrified) state, rather than an ordered, destructive state of crystalline ice. Vitrification can be described as a rapid increase in fluid viscosity that traps water molecules along random orientations. However, the vitrification medium may contain relatively high levels of cryoprotectant, which can be toxic to cells except in the vitrified state. Therefore, the time cells are exposed to the vitrification medium during dehydration and warming must be carefully controlled to avoid cell damage, and thus freezing should be performed as quickly as possible.

[0005] The CRYOLOCK cryopreservation device was developed as a versatile, simple, and efficient vitrification apparatus designed for the preservation, freezing, and storage of oocytes or embryos in liquid nitrogen. It is a cryopreservation device that allows samples to be rapidly cooled and stored without direct contact with sterile liquid nitrogen (LN2). The CRYOLOCK cryopreservation device is described in U.S. Patent Application Publication No. 2016 / 0174545, published June 23, 2016, which is incorporated herein by reference in its entirety.

[0006] The CRYOLOCK cryopreservation device uses a combination of semi-flexible plastic and functional design, including specific shapes and tight gaps, to hermetically seal samples within the cryopreservation device at room temperature and maintain this hermetically sealed state as the cryopreservation device and samples cool to liquid nitrogen temperatures.

[0007] Due to the cost of storing samples in liquid nitrogen, multiple samples are stored in a given cryogenic storage container. Since the entire cryopreservation unit is stored within the container, the size of each unit and the packaging characteristics of such units affect the amount of space required and thus storage costs. Beyond simple space considerations, quality management and auditing are necessary to ensure that the identification and storage conditions of each sample are tracked over time. Temperature fluctuations can affect the long-term viability of samples if a cryopreservation unit needs to be removed during a quality audit.

[0008] There is a need to be able to identify each sample / device in a cryopreservation tank without removing it from the tank. It is necessary to maintain or reduce the size of self-identifiable cryopreservation devices, particularly at the level of individual biological samples. The ability to identify each sample / device needs to be maintained throughout its entire lifecycle of collection, storage, retrieval, and use.

[0009] Integrating RFID tags into each cryopreservation unit presents several challenges. First, the portion of the unit that holds the biological sample must be sterilized, typically using gamma rays, which can impair the functionality of the RFID tags. Furthermore, the RFID tag integration must be compatible with the workflow during vitrification, meaning the tag needs to be securely attached to a portion of the cryopreservation unit before biological sample collection, given the inevitably short time between sample collection and immersion in liquid nitrogen. The RFID tag needs to be firmly held by the cryopreservation unit under environmental conditions and liquid nitrogen temperature without damaging the unit during such large temperature fluctuations. Any dimensional increases within the cryopreservation unit that can hold the RFID tag need to be minimized. Ideally, the RFID tag should be attached to the cryopreservation unit without the need for adhesives that may be affected by low temperatures. Summary of the Invention

[0010] According to an embodiment of the present invention, a cryopreservation device is provided capable of retaining a Radio Frequency Identification (RFID) tag. The cryopreservation device includes: a) an elongated rod comprising an RFID segment, an elongated body, and a sample collection tip; and b) a cap comprising a hollow chamber having a length sufficient to accommodate the sample collection tip. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber. The RFID segment is located away from the sample collection tip and is capable of retaining the RFID tag.

[0011] According to another embodiment of the present invention, a cryogenic preservation device capable of retaining an RFID tag is provided. The cryogenic preservation device includes: a) an elongated rod including an RFID segment, an elongated body, a frustoconical boss extending from a first end of the elongated body, and a sample collection tip extending from the frustoconical boss; and b) a cap including a hollow chamber having a length sufficient to accommodate the sample collection tip and the frustoconical boss. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the frustoconical boss within the hollow chamber. The RFID segment is located away from the sample collection tip and is capable of retaining the RFID tag. The RFID segment includes: a) an RFID chamber capable of retaining the RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening.

[0012] According to another embodiment of the present invention, a cryogenic preservation device capable of retaining an RFID tag is provided. The cryogenic preservation device includes: a) an elongated rod including an RFID segment, an elongated body, a frustoconical boss extending from a first end of the elongated body, and a sample collection tip extending from the frustoconical boss; and b) a cap including a hollow chamber having a length sufficient to accommodate the sample collection tip and the frustoconical boss. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber. The RFID segment is located away from the sample collection tip and is capable of retaining the RFID tag. The RFID segment includes: a) an RFID chamber capable of retaining the RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening. The elongated rod is made of a single plastic piece. The RFID opening is located at the end of the elongated rod. The RFID chamber comprises a cylindrical cavity made of plastic with a prismatic profile, and the RFID tag is cylindrical. The prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit originating from the RFID opening. The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. The first longitudinal slit extends across the RFID chamber from the second longitudinal slit. The locking mechanism includes a platform at least partially surrounding the periphery of the RFID opening.

[0013] According to another embodiment of the present invention, a method for vitrifying biological samples is provided. The method includes: a) acquiring a cryogenic preservation device according to any of the above embodiments; b) acquiring a liquid nitrogen-resistant RFID tag; c) placing the RFID tag into the RFID chamber; reading the RFID tag and associating the RFID tag with the biological sample; e) adding a vitrification mixture to dehydrate the biological sample; f) collecting the dehydrated biological sample on the elongated rod; g) sealing the dehydrated biological sample by fixing the cap to the elongated rod; and h) placing the cryogenic preservation device in liquid nitrogen. Attached Figure Description

[0014] This invention is illustrated and described with reference to various accompanying drawings, wherein similar reference numerals denote similar method steps and / or system components, and in the drawings:

[0015] Figure 1aThis is a side view of the cryogenic storage device compared to Example 1;

[0016] Figure 1b yes Figure 1a A cross-sectional view of the cryogenic storage device along line AA;

[0017] Figure 1c There is no hat. Figure 1a Side view of the cryogenic storage device;

[0018] Figure 1d yes Figure 1c A cross-sectional view of the cryogenic storage device along line AA;

[0019] Figure 2a This is a side view of an exemplary embodiment of the cryogenic storage device in Example 1;

[0020] Figure 2b yes Figure 2a A cross-sectional view along line AA of the illustrated embodiment;

[0021] Figure 2c yes Figure 2a A side view of the opposite side (rotated 180 degrees along the longitudinal axis) of the illustrated embodiment;

[0022] Figure 2d yes Figure 2a Isometric diagram of the RFID segment in the illustrated embodiment;

[0023] Figure 3a This is a side view of an exemplary embodiment of the cryogenic storage device of Example 2;

[0024] Figure 3b yes Figure 3a Top view of the RFID segment and the end of the slender rod in the embodiment shown;

[0025] Figure 3c yes Figure 3a Isometric view of the RFID segment and the end of the slender rod in the illustrated embodiment;

[0026] Figure 4a This is a side view of the cryogenic storage device in Comparative Example 2;

[0027] Figure 4b yes Figure 4a A cross-sectional view of the cryogenic storage device shown;

[0028] Figure 4c yes Figure 4a A detailed cross-sectional view of the RFID section of the cryogenic storage device shown;

[0029] Figure 5a This is a side view of an exemplary embodiment of the cryogenic storage device in Example 3;

[0030] Figure 5b yes Figure 5a A cross-sectional view of the embodiment shown;

[0031] Figure 5c yes Figure 5a Detailed cross-sectional view of the RFID segment in the illustrated embodiment;

[0032] Figure 6a This is a side view of another embodiment of a cryogenic storage device having an end-loaded RFID segment and a plug for a locking mechanism;

[0033] Figure 6b yes Figure 6a A cross-sectional view of the RFID segment, the RFID tag not yet loaded, and the plug removed from the RFID segment in the illustrated embodiment.

[0034] Figure 6c yes Figure 6a A cross-sectional view of the RFID segment of the embodiment shown, wherein the RFID tag is securely housed within the RFID chamber;

[0035] Figure 7 This is a cross-sectional view of another embodiment of a cryogenic storage device having an end-loaded RFID segment;

[0036] Figure 8a This is a cross-sectional view of another embodiment of a cryogenic storage device with a detachable RFID segment;

[0037] Figure 8b This is a cross-sectional view of the detachable RFID section. Figure 8a The elongated body of the embodiment shown is in a disassembled state;

[0038] Figure 8c It is a cross-sectional view of an alternative connection configuration for a cryogenic storage device and a corresponding detachable RFID segment.

[0039] Figure 9a This is a side view of another embodiment of a cryogenic storage device with a top-loaded RFID section;

[0040] Figure 9b yes Figure 9a A cross-sectional view of the RFID segment in the illustrated embodiment;

[0041] Figure 9c yes Figure 9a A detailed top view of the RFID segment in the illustrated embodiment; and

[0042] Figure 9d yes Figure 9c Different cross-sectional views of the RFID segment in the illustrated embodiment. Detailed Implementation

[0043] Embodiments of the present invention provide a cryogenic preservation device capable of retaining a radio frequency identification (RFID) tag. The cryogenic preservation device includes: a) an elongated rod comprising an RFID segment, an elongated body, and a sample collection tip; and b) a cap comprising a hollow chamber having a length sufficient to accommodate the sample collection tip. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow chamber. The RFID segment is located away from the sample collection tip and is capable of retaining the RFID tag.

[0044] The invention can be more readily understood by referring to the following detailed description of the invention, which forms part of this disclosure, in conjunction with the accompanying drawings. It should be understood that the invention is not limited to the specific apparatus, methods, conditions, or parameters described and / or shown herein, and the terminology used herein is for the purpose of describing particular embodiments by way of example only and is not intended to limit the claimed invention. Any and all patents and other disclosures identified in this specification are incorporated by reference as if fully set forth herein.

[0045] Furthermore, unless the context explicitly specifies otherwise, as used in the specification, including the appended claims, the singular forms “a,” “an,” and “described” include the plural, and references to a particular numerical value include at least that particular value. A range herein may be expressed as from “about” or “approximately” one particular value and / or to “about” or “approximately” another particular value. When such a range is expressed, another embodiment includes from that one particular value and / or to that other particular value. Similarly, when a value is expressed as an approximation, the particular value will be understood to form another embodiment by using the antecedent “about.”

[0046] It should be understood that mentioning one or more method steps does not preclude the presence of additional method steps before or after the described combination of steps, or the existence of intermediate method steps between those explicitly identified steps. Furthermore, the coded text for method steps or components is a conventional means of identifying discrete activities or components, and unless otherwise stated, the described coded texts may be arranged in any order. Method steps may begin with the word "optional," but this does not require the appearance of such a method step.

[0047] As used herein, when the term “and / or” is used in a list of two or more items, it means that any one of the listed items may be used alone, or any combination of the listed items or two or more items may be used alone. For example, if a composition is described as containing compounds A, B, and / or C, the composition may contain: A alone; B alone; C alone; a combination of A and B; a combination of A and C; a combination of B and C; or a combination of A, B, and C.

[0048] RFID tags are not particularly limited, provided they are compatible with liquid nitrogen. For liquid nitrogen compatibility, RFID tags function as intended under ambient conditions and when immersed in liquid nitrogen (and thus at its temperature). RFID tags function as intended when they can receive queries from an RFID reader and send their unique identifier back to the reader. In some aspects, an RFID tag is an object containing an integrated circuit, an antenna, and a substrate. In some aspects, the substrate comprises silicon and / or carbon fiber.

[0049] In some aspects, the cryopreservation device further includes a truncated conical boss extending from a first end of the elongated body. In some aspects, the sample collection tip extends from the truncated conical boss. In some aspects, when the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip within the hollow cavity. In some aspects, when the cap is detachably attached to the elongated rod, the cap is capable of hermetically enclosing the sample collection tip within the hollow cavity.

[0050] In some aspects, the RFID segment includes: a) an RFID chamber capable of holding an RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening.

[0051] In some aspects, the elongated rod is made from a single piece of plastic. For example, when the elongated rod is injection molded in a mold that forms the RFID segment, the elongated body, the sample collection tip, and any other components of the elongated rod, it is made from a single piece of plastic. In another example, when the elongated rod is 3D printed, it is made from a single piece of plastic.

[0052] In some aspects, when the RFID tag is placed in the RFID chamber and the cryogenic storage device is placed in liquid nitrogen for 5 minutes, the RFID tag remains in the RFID chamber, and the cryogenic storage device does not show any visible cracks. In some aspects, when the RFID tag is placed in the RFID chamber and the cryogenic storage device is placed in liquid nitrogen for 5 minutes, the RFID tag remains in the RFID chamber, and the cryogenic storage device does not show any cracks when viewed at 10x magnification.

[0053] In some aspects, the shape of the RFID chamber corresponds to the shape of the RFID tag. In some aspects, the RFID tag is cube-shaped, cuboid-shaped, cone-shaped, cylinder-shaped, sphere-shaped, pyramid-shaped, or prism-shaped. In some aspects, the RFID tag is cylindrical or cylindrical in shape. In some aspects, the length of the RFID tag ranges from 4 mm to 12 mm. In some aspects, the RFID tag has a diameter ranging from 0.5 mm to 4 mm, 0.5 mm to 3.5 mm, or 0.5 mm to 3 mm, or 0.5 mm to 2.5 mm, or 0.05 mm to 2.2 mm, or 0.5 mm to 2 mm, or 0.5 mm to 1.8 mm, or 0.5 mm to 1.4 mm, or 0.5 mm to 1 mm.

[0054] The additional volume, width, and / or length of the RFID chamber relative to the RFID tag allows the walls of the RFID chamber to contract when immersed in liquid nitrogen, while ensuring that the more rigid RFID tag does not cause the walls of the RFID chamber to crack. In some aspects, the volume of the RFID chamber is 1.01 times the volume of the RFID tag. Other non-limiting examples include the volume of the RFID chamber being at least 1.02 times, or 1.03 times, or 1.04 times, or 1.05 times, or 1.1 times the volume of the RFID tag. In some aspects, the width of the RFID chamber perpendicular to the longitudinal direction of the slender rod is at least 1.01 times, or 1.02 times, or 1.03 times, or 1.04 times, or 1.05 times, or 1.1 times the corresponding width of the RFID tag. In some respects, the length of the RFID chamber along the longitudinal direction of the slender rod is at least 1.01 times, or 1.02 times, or 1.03 times, or 1.04 times, or 1.05 times, or 1.1 times the length of the RFID tag.

[0055] In some aspects, the RFID opening is located at the end of the elongated rod. In some aspects, the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prism-shaped profile. In some aspects, the prism shape is cuboid. In some aspects, the prism shape is hexagonal (prismatic). In some aspects, the RFID tag is substantially cylindrical, and wherein the prism shape has a first longitudinal slit and a second longitudinal slit, each slit originating from the RFID opening. In some aspects, the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. In some aspects, the first longitudinal slit is longer than the second longitudinal slit. In some aspects, the first longitudinal slit traverses the RFID chamber from the second longitudinal slit.

[0056] In some aspects, the RFID segment has a dimension of no more than about 5 mm in each of the x and y directions perpendicular to the longitudinal direction. In other examples, the RFID segment has a dimension of no more than about 4.5 mm, or 4.0 mm, or 3.5 mm, or 3.1 mm, or 3.0 mm, or 2.8 mm, or 2.6 mm, or 2.4 mm in each of the x and y directions perpendicular to the longitudinal direction.

[0057] In some aspects, the RFID opening is positioned along the longitudinal direction of the elongated rod. The RFID segment includes a locking mechanism adjacent to the periphery of the RFID opening. In some aspects, the locking mechanism includes a platform at least partially surrounding the periphery of the RFID opening. In some aspects, the length of the RFID opening is less than the length of the RFID tag, and the locking mechanism includes the RFID segment adjacent to the periphery of the RFID opening and covering a portion of the RFID chamber. In some aspects, the RFID tag can be loaded into the RFID chamber at an angle to allow a portion of the RFID tag to enter the portion of the RFID chamber beyond the RFID opening, and then push the remaining portion of the RFID tag through the RFID opening.

[0058] In some aspects, the length of the RFID opening is along the longitudinal axis of the elongated rod, wherein the length of the RFID opening is shorter than the length of the RFID chamber. In some aspects, the locking mechanism includes a circumferential flap that curves inward toward the RFID chamber. In some aspects, the locking mechanism further includes an obtrusion connected to the circumferential flap and projecting above the RFID opening. In some aspects, the RFID chamber has a dimension perpendicular to the longitudinal direction, between 1.01% and 1.1% of the same size as the RFID tag.

[0059] Another embodiment of the present invention provides a cryogenic preservation device capable of retaining an RFID tag. The cryogenic preservation device includes: a) an elongated rod including an RFID segment, an elongated body, a frustoconical boss extending from a first end of the elongated body, and a sample collection tip extending from the frustoconical boss; and b) a cap including a hollow chamber having a length sufficient to accommodate the sample collection tip and the frustoconical boss. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the frustoconical boss within the hollow chamber. The RFID segment is located away from the sample collection tip and is capable of retaining the RFID tag. The RFID segment includes: a) an RFID chamber capable of retaining the RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening.

[0060] It should be understood that the elongated rod, RFID segment, RFID tag, RFID chamber, RFID opening, RFID locking mechanism, manufacturing of the elongated rod, shape and size of the RFID tag, RFID chamber and RFID opening, relative dimensions of various aspects of the RFID chamber to the RFID tag, placement of the RFID opening, any longitudinal slit along the wall of the RFID chamber, and various aspects of the dimensions of the RFID segment in each of the x and y directions perpendicular to the longitudinal direction also apply to this embodiment.

[0061] In some aspects, the elongated rod is made of a single plastic piece. In some aspects, the RFID tag is cylindrical and has a length ranging from 6 mm to 12 mm and a diameter ranging from 0.5 mm to 4 mm. Other non-limiting examples of RFID tag diameters are from 0.5 mm to 3.5 mm, or 0.5 mm to 3 mm, or 0.5 mm to 2.5 mm, or 0.05 mm to 2.2 mm, or 0.5 mm to 2 mm, or 0.5 mm to 1.8 mm.

[0062] In some aspects, the RFID opening is located at the end of the elongated rod. In some aspects, the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prism-shaped profile. In some aspects, the prism shape is cuboid. In some aspects, the prism shape is hexagonal. In some aspects, the RFID tag is substantially cylindrical. In some aspects, the prism shape has a first longitudinal slit and a second longitudinal slit, each of the first and second longitudinal slits originating from the RFID opening. In some aspects, the locking mechanism includes a platform at least partially surrounding the RFID opening.

[0063] In some aspects, the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. In some aspects, the first longitudinal slit is longer than the second longitudinal slit. In some aspects, the first longitudinal slit extends across the RFID chamber from the second longitudinal slit.

[0064] In some aspects, the RFID segment has a dimension of no more than about 5 mm in each of the x and y directions perpendicular to the longitudinal direction. In other examples, the RFID segment has a dimension of no more than about 4.5 mm, or 4.0 mm, or 3.5 mm, or 3.1 mm, or 3.0 mm, or 2.8 mm, or 2.6 mm, or 2.4 mm in each of the x and y directions perpendicular to the longitudinal direction.

[0065] Another embodiment of the present invention provides a cryogenic preservation device capable of holding an RFID tag. The cryogenic preservation device includes: a) an elongated rod including an RFID segment, an elongated body, a frustoconical boss extending from a first end of the elongated body, and a sample collection tip extending from the frustoconical boss; and b) a cap including a hollow chamber having a length sufficient to accommodate the sample collection tip and the frustoconical boss. When the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the frustoconical boss within the hollow chamber. The RFID segment is located away from the sample collection tip and is capable of holding the RFID tag. The RFID segment includes: a) an RFID chamber capable of holding the RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening. The elongated rod is made of a single plastic piece. The RFID opening is located at the end of the elongated rod. The RFID chamber comprises a cylindrical cavity made of plastic with a prismatic profile, and the RFID tag is cylindrical. The prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit originating from the RFID opening. The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. The first longitudinal slit extends across the RFID chamber from the second longitudinal slit. The locking mechanism includes a platform that at least partially surrounds the periphery of the RFID opening.

[0066] It should be understood that the elongated rod, RFID segment, RFID tag, RFID chamber, RFID opening, RFID locking mechanism, manufacturing of the elongated rod, shape and size of the RFID tag, RFID chamber and RFID opening, relative dimensions of various aspects of the RFID chamber to the RFID tag, placement of the RFID opening, any longitudinal slit along the wall of the RFID chamber, and various aspects of the dimensions of the RFID segment in each of the x and y directions perpendicular to the longitudinal direction also apply to this embodiment.

[0067] According to another embodiment of the present invention, a process for vitrifying biological samples is provided. The process includes the following steps: a) obtaining a cryogenic preservation device according to any of the above embodiments; b) obtaining a liquid nitrogen-resistant RFID tag; c) placing the RFID tag into the RFID chamber; reading the RFID tag and associating the RFID tag with the biological sample; e) adding a vitrification mixture to dehydrate the biological sample; f) collecting the dehydrated biological sample on the elongated rod; sealing the dehydrated biological sample by fixing the cap to the elongated rod; and h) placing the cryogenic preservation device in liquid nitrogen.

[0068] It should be understood that the elongated rod, RFID segment, RFID tag, RFID chamber, RFID opening, RFID locking mechanism, manufacturing of the elongated rod, shape and size of the RFID tag, RFID chamber and RFID opening, relative dimensions of various aspects of the RFID chamber to the RFID tag, placement of the RFID opening, any longitudinal slit along the wall of the RFID chamber, and various aspects of the dimensions of the RFID segment in each of the x and y directions perpendicular to the longitudinal direction also apply to this embodiment.

[0069] In some aspects, the RFID segment did not show any visible cracks when placed in liquid nitrogen for 5 minutes.

[0070] The following is a non-limiting list of embodiments.

[0071] A1. A cryogenic preservation device, comprising:

[0072] a) A slender rod, said slender rod comprising an RFID segment, a slender body, and a sample collection tip; and

[0073] b) A cap comprising a hollow chamber having a length sufficient to accommodate the sample collection tip.

[0074] When the cap is detachably attached to the elongated rod, it can enclose the sample collection tip within the hollow cavity, and

[0075] The RFID segment is located far from the sample collection endpoint and is capable of holding the RFID tag.

[0076] A2. The cryogenic preservation device according to any embodiment of embodiment A1 further includes a truncated conical protrusion extending from a first end of the elongated body, wherein a sample collection tip extends from the truncated conical protrusion, and wherein when the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the truncated conical protrusion within the hollow cavity.

[0077] A3. The cryogenic preservation device according to any one of embodiments A1 or A2, wherein the RFID section includes: a) an RFID chamber capable of holding an RFID tag; b) an RFID opening through which the RFID tag can be placed into the RFID chamber; and c) a locking mechanism adjacent to the periphery of the RFID opening.

[0078] A4. The cryogenic preservation device according to any one of embodiments A1 to A3, wherein the elongated rod is made of a single plastic piece; or wherein the elongated rod is produced by injection molding polymer resin into a single mold; or wherein the elongated rod is produced by 3D printing.

[0079] A5. According to any one of the embodiments A1 to A4, when the RFID tag can be placed in the RFID chamber and the cryogenic preservation device can be placed in liquid nitrogen for 5 minutes, the RFID tag remains in the RFID chamber, and the cryogenic preservation device does not show any visible cracks, and / or when observed at 10X magnification, the cryogenic preservation device does not show any cracks.

[0080] A6. The cryogenic preservation device according to any one of embodiments A1 to A5, wherein the RFID tag is cylindrical and has a length ranging from 4 mm to 12 mm, and a diameter ranging from 0.5 mm to 4 mm, 0.5 mm to 3 mm, 0.5 mm to 2.5 mm, 0.5 mm to 2 mm, or 0.5 mm to 1.8 mm.

[0081] A7. A cryogenic preservation device according to any one of embodiments A3 to A6, wherein the RFID opening is located at the end of the elongated rod, wherein the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prism-shaped profile, wherein the RFID tag is substantially cylindrical, and wherein the prism shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening.

[0082] A8. The cryogenic preservation apparatus according to embodiment A7, wherein the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, and wherein the first longitudinal slit is longer than the second longitudinal slit.

[0083] A9. The cryogenic preservation device according to any one of embodiments A7 or A8, wherein the first longitudinal slit extends transversely through the RFID chamber from the second longitudinal slit.

[0084] A10. A cryogenic preservation apparatus according to any one of embodiments A3 to A9, wherein the locking mechanism includes a platform that at least partially surrounds the periphery of the RFID opening.

[0085] A11. The cryogenic preservation device according to any one of embodiments A1 to A10, wherein the size of the RFID segment in each of the x and y directions perpendicular to the longitudinal direction is not greater than about 5 mm, or 4.5 mm, or 4.0 mm, or 3.5 mm, or 3.1 mm, or 3.0 mm, or 2.8 mm, or 2.6 mm.

[0086] A12. The cryogenic preservation device according to any one of embodiments A1 to A11, wherein the RFID segment has a dimension of no more than about 2.4 mm in each of the x and y directions perpendicular to the longitudinal direction.

[0087] A13. The cryogenic preservation apparatus according to any one of embodiments A3 to A6, wherein the length of the RFID opening is along the longitudinal axis of the elongated rod.

[0088] A14. The cryogenic preservation device according to embodiment A13, wherein the length of the RFID opening is shorter than the length of the RFID chamber.

[0089] A15. A cryogenic preservation device according to any one of embodiments A13 or A14, wherein the locking mechanism includes a circumferential flap that bends inward toward the RFID chamber.

[0090] A16. The cryogenic preservation device according to embodiment A15, wherein the locking mechanism further includes a protrusion connected to the circumferential flap and protruding above the RFID opening.

[0091] A17. The cryogenic preservation apparatus according to any one of embodiments A13 to A17, wherein the RFID chamber has a dimension perpendicular to the longitudinal direction, which is between 1.01% and 1.1% of the same size as the RFID tag.

[0092] A18. The cryogenic preservation device according to any one of embodiments A13 to A17, wherein the dimension of the RFID segment in each of the x-direction and y-direction perpendicular to the longitudinal direction is no greater than about 3 mm or no greater than about 2.4 mm.

[0093] A19. The cryogenic preservation apparatus according to any one of embodiments A1 to A18, wherein the RFID tag is liquid nitrogen compatible.

[0094] A20. A cryogenic preservation device according to any one of embodiments A1 to A19, wherein the cryogenic preservation device includes at least one circumferential groove.

[0095] A21. The cryogenic preservation device according to any one of embodiments A1 to A20, wherein when the cap is detachably attached to the elongated rod, the cap is capable of airtightly sealing the sample collection tip within the hollow cavity.

[0096] A22. A cryogenic preservation apparatus according to any one of embodiments A3 to A6, A11, A12 or A17 to A21, wherein the RFID opening is located at the end of the elongated rod, and the locking mechanism includes a plug capable of blocking the RFID opening.

[0097] A23. A process for vitrifying biological samples, the process comprising:

[0098] a) Obtain the cryogenic preservation apparatus according to any one of embodiments A1 to A20;

[0099] b) Obtain the RFID tag;

[0100] c) Place the RFID tag into the RFID chamber;

[0101] d) Read the RFID tag and associate the RFID tag with the biological sample;

[0102] e) Add the vitrification mixture to dehydrate the biological sample;

[0103] f) Collect dehydrated biological samples at the sample collection tip;

[0104] g) The dehydrated biological sample is sealed by detachably attaching the cap to the elongated rod;

[0105] h) Place the cryogenic storage device into liquid nitrogen.

[0106] Example

[0107] Comparative Examples 1, 2, and 3 were performed using elongated rods of cryopreservation devices injection-molded from polystyrene. Examples 1 and 2 were performed using prototypes of the elongated rods of the cryopreservation devices. The prototypes were 3D printed by Statasys' Digital ABS Plus from Eden Prairie, Minnesota. The elongated rods of each device / prototype were made from a single monolithic plastic piece. The elongated rods in each example were designed with an RFID chamber to house a transponder RFID microchip tag (RFID tag), which was cylindrical with a diameter of 2 mm and a length of 12 mm and was available from Shenzhen Manruta Technology Co., Ltd. in Guangdong Province, China. The sample collection tip of each elongated rod used for the comparative examples and examples was sealed with a CRYOLOCK cap (Biotech, Inc., Alpharetta, GA).

[0108] Comparison Example 1

[0109] Design and injection molding, such as Figures 1a to 1d The cryogenic storage device shown. (Reference) Figure 1a , Figure 1b , Figure 1c and Figure 1d The cryopreservation device 10 includes an elongated rod 12 and a cap 14. The elongated rod includes an RFID section 16, a circumferential groove 18a, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the boss 22. The cap 14 includes a circumferential groove 18b and an elongated hollow chamber 25 defined along the long axis of the cap 14 and sized to accommodate the sample collection tip 24 and the frustoconical boss 22. The circumferential grooves 18a and 18b, located near the ends of the cap 14 and the distal ends of the elongated rod 12, are used to clamp the cryopreservation device 10 using tweezers (not shown), thereby making the cryopreservation device 10 easier to manipulate in varying temperature environments. The RFID section 16 includes an RFID transition area 26, an RFID chamber 28, and an RFID terminal area 30. The RFID terminal area 30 is positioned further away from the elongated body 20 along the longitudinal axis than the RFID transition area 26, and the RFID chamber 28 is located between the RFID terminal area 30 and the RFID transition area 26. The dimensions of the RFID chamber 28 are designed to hold an RFID tag (not shown). Thus, the RFID chamber 28 is cylindrical, and the opening 32 is circular. Figure 1c and Figure 1dFigure 1 shows a side view and a cross-sectional view of the cryogenic storage device 10, with the cap 14 removed, thus revealing the entire elongated rod 12.

[0110] Under ambient conditions, the RFID tag is manually inserted into the RFID chamber 28 through opening 32. The RFID tag is firmly held in the RFID chamber 28 and the chip cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. The elongated rod 12 is immersed in liquid nitrogen for approximately 2 minutes. When removed from the liquid nitrogen, the elongated rod 12 is inspected. The RFID chamber 28 has several visible cracks.

[0111] Example 1

[0112] Design as Figures 2a to 2d The slender rod 12 shown is for a cryogenic storage device, and a prototype was created using 3D printing. (Reference) Figure 2a , Figure 2b , Figure 2c and Figure 2d The elongated rod 12 includes an RFID segment 16, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The RFID segment 16 includes a tapered RFID transition region 26, an RFID chamber 28, and an RFID terminal region 30. The RFID terminal region 30 is positioned further away from the elongated body 20 along the longitudinal axis than the RFID transition region 26, and the RFID chamber 28 is located between the RFID terminal region 30 and the tapered RFID transition region 26. Because the RFID chamber 28 has a larger cross-section than the elongated body 20, the RFID transition region 26 is tapered or gradually tapers. The dimensions of the RFID chamber 28 are designed to hold an RFID tag (not shown).

[0113] An RFID tag is manually inserted into the RFID chamber 28 through an opening 32, the opening of which is sized to allow the RFID tag to pass through and enter the RFID chamber 28. A locking mechanism 34 defines the opening 32. Figure 2a and Figure 2d The locking mechanism 34 shown ensures that the RFID tag (not shown) remains inside the RFID chamber 28 throughout the entire process of handling and storing the elongated rod 12. The RFID tag is inserted at an angle that allows a portion of the RFID tag to enter the portion of the RFID chamber 28 beyond the RFID opening 32 and adjacent to the RFID terminal area 30. Then, as the RFID tag is pushed into the RFID chamber 28, once the RFID tag is fully inserted, flaps 36a and 36b bend downwards and rest on top of the RFID tag. Figure 2cAs shown, openings 37a and 37b are longitudinally spaced along the outer edge of the RFID chamber 28, and opening 32 is positioned on the opposite side of the elongated rod 12, perpendicular to the longitudinal direction. The RFID tag is securely held in the RFID chamber 28 and the chip cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. The elongated rod 12 is immersed in liquid nitrogen. The RFID segment 16 is inserted into the liquid nitrogen for approximately 2 minutes and then returned to ambient conditions; the RFID segment 16 shows no visible signs of breakage. The RFID segment 16 is observed at 10x magnification, and still no signs of breakage are observed.

[0114] Example 2

[0115] Design as Figures 3a to 3c The elongated rod 12 for a cryogenic storage device is shown, and a 3D-printed prototype was also created. The elongated rod 12 includes a groove 18a, an RFID segment 16, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The RFID segment 16 includes a tapered RFID transition area 26, an RFID chamber 28, and an RFID terminal area 30. The RFID terminal area 30 is positioned further away from the elongated body 20 along the longitudinal axis than the RFID transition area 26, and the RFID chamber 28 is located between the RFID terminal area 30 and the RFID transition area 26. The RFID chamber 28 is sized to hold an RFID tag 38. The tapered RFID transition area 26 is designed to transition from a smaller cross-section of the elongated body 20 to a larger cross-section of the RFID segment 16, while allowing the resting side of the elongated body 22 and the RFID segment 16 to be straight for easy handling.

[0116] Under ambient conditions, the RFID tag is manually inserted into the RFID chamber 28 through the opening 32. The RFID tag is inserted at an angle that allows a portion of the RFID tag to enter the portion of the RFID chamber 28 beyond the RFID opening 32 and adjacent to the RFID terminal area 30. The locking mechanism 34 includes a circular tag 39 that bends downwards when the RFID tag 38 is loaded into the RFID chamber 28 and is positioned above the RFID tag 38 after it is fully inserted into the RFID chamber 28. The locking mechanism 34 retains the RFID tag 38 within the RFID chamber 28. The RFID tag is securely held within the RFID chamber 28 and will not be removed from the RFID chamber 28 by shaking the elongated rod 12. The elongated rod 12 is immersed in liquid nitrogen. When the elongated rod 12 is immersed in liquid nitrogen for approximately 2 minutes and then returned to ambient conditions, the RFID segment 16 shows no visible signs of breakage. The RFID segment 16 was observed at 10x magnification, and no signs of breakage were still observed.

[0117] Comparison Example 2

[0118] Design and injection mold slender rod 12. Reference Figure 4a , Figure 4b ,and Figure 4c The elongated rod includes an RFID segment 16, a circumferential groove 18a, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The circumferential groove 18a is located between the end of the elongated body 20 away from the sample collection tip 24 and the RFID segment 16. The RFID segment 16 includes an RFID transition area 26, an RFID chamber 28, and an RFID terminal area 30. The RFID terminal area 30 is positioned further away from the elongated body 20 along the longitudinal axis than the RFID transition area 26, and the RFID chamber 28 is located between the RFID terminal area 30 and the RFID transition area 26. The RFID chamber 28 is sized to hold an RFID tag (not shown). Thus, the RFID chamber 28 is cylindrical, and the opening 32 is circular. Figure 4a The diagram shows one of the two longitudinal slits, longitudinal slit 40a, and... Figure 4b and Figure 4c Two longitudinal slits 40a and 40b are shown. In this comparative example, the longitudinal slits are of the same size, and each slit begins at the RFID opening 32 and removes a piece of approximately 90% of the length of the wall of the RFID chamber 28. Figure 4b yes Figure 4a The slender rod 12 shown is a cross-sectional view, and Figure 4cyes Figure 4a A detailed cross-sectional view of the RFID section 16 of the elongated rod 12 shown. The locking mechanism includes an "elbow" or ledge 42a, 42b on at least a portion of the wall of the RFID chamber 28 closest to the RFID opening 32.

[0119] Under ambient conditions, an RFID tag is inserted into the RFID chamber 28 through opening 32. The RFID tag cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. However, since the RFID tag can be removed from the RFID chamber 28 by simply moving a block of the wall of the RFID chamber 28 outwards, the RFID tag is not securely held in the RFID chamber 28. The elongated rod 12 with the RFID tag in the RFID chamber 28 is then immersed in liquid nitrogen. The RFID segment 16 is inserted into the liquid nitrogen for approximately 2 minutes and then returned to ambient conditions; the RFID segment 16 shows no visible signs of breakage. The RFID segment 16 is observed at 10x magnification, and still no signs of breakage are observed. This design addresses only one of the two necessary criteria: the RFID segment 16 does not break, but the RFID tag is not securely held in place during handling in the cryogenic storage device.

[0120] Example 3

[0121] Design and injection mold slender rod 12. Reference Figure 5a , Figure 5b and Figure 5c The elongated rod includes an RFID segment 16, a circumferential groove 18a, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The circumferential groove 18a is located between the end of the elongated body 20 away from the sample collection tip 24 and the RFID segment 16. The RFID segment 16 includes an RFID transition area 26, an RFID chamber 28, and an RFID terminal area 30. The RFID terminal area 30 is positioned further away from the elongated body 20 along the longitudinal axis than the RFID transition area 26, and the RFID chamber 28 is located between the RFID terminal area 30 and the RFID transition area 26. The RFID chamber 28 is sized to hold an RFID tag (not shown). Thus, the RFID chamber 28 is cylindrical, and the opening 32 is circular. Figure 5a The diagram shows one of the two longitudinal slits, longitudinal slit 40a, and... Figure 5b , Figure 5cThe image shows two longitudinal slits 40a and 40b. In this example, the longitudinal slits are of different sizes, and longitudinal slit 40a removes approximately 50% of the length of the wall of the RFID chamber 28, while longitudinal slit 40b removes approximately 90% of the length of the wall of the RFID chamber 28. Figure 5b yes Figure 5a The slender rod 12 shown is a cross-sectional view, and Figure 5c yes Figure 5a A detailed cross-sectional view of the RFID section 16 of the elongated rod 12 shown. The locking mechanism includes "bends" or platforms 42a, 42b on at least a portion of the wall of the RFID chamber 28 closest to the RFID opening 32.

[0122] Under ambient conditions, an RFID tag is inserted into the RFID chamber 28 through opening 32. The RFID tag is firmly held in the RFID chamber 28 and cannot be removed from the RFID chamber 28 by shaking the elongated rod 12. Unlike Comparative Example 2, the RFID tag cannot be removed from the RFID chamber 28 by moving the wall of the RFID chamber 28 outward. The elongated rod 12 is immersed in liquid nitrogen. The RFID segment 16 is inserted into the liquid nitrogen for approximately 2 minutes and then returned to ambient conditions; the RFID segment 16 shows no visible signs of breakage. The RFID segment 16 is observed at 10x magnification, and still no signs of breakage are observed.

[0123] Figure 6a Another embodiment of a closed-system cryopreservation device 10 is illustrated, which has an elongated rod 12 and a cap 14 (not shown) with an end-loaded RFID segment 16. The elongated rod 12 includes the RFID segment 16, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The RFID segment 16 includes an RFID transition area 26, an RFID compartment 28, and an RFID terminal area 30. An RFID plug 44 is detachably attached to the RFID segment 16. The RFID terminal area 30 is positioned further away from the elongated body 20 along the longitudinal axis than the RFID transition area 26, and the RFID compartment 28 is located between the RFID terminal area 30 and the RFID transition area 26.

[0124] Figure 6b and Figure 6c yes Figure 6a A detailed cross-sectional view of the RFID segment 16 in the illustrated embodiment. Figure 6b This is a cross-sectional view of the empty RFID chamber 28, in which the RFID tag 38 and RFID plug 44 are separated from the RFID section 16. Figure 6cThis is a cross-sectional view showing the RFID tag 38 located within the RFID chamber 28 and secured by the RFID plug 44. In this embodiment, the RFID chamber 28 is slightly oversized relative to the RFID tag 38 to allow for thermal shrinkage when the cryogenic storage device is immersed in liquid nitrogen. The RFID plug 44 is used for... Figures 2a to 2d and Figures 3a to 3c The locking mechanism 34 shown in the embodiment serves the same purpose. By blocking the RFID opening 32, the RFID plug 44 retains the RFID tag 38 in the RFID chamber 28 throughout the handling and storage of the cryogenic storage device.

[0125] Figure 7 Another embodiment of a cryopreservation device 10 is shown, which has an elongated rod 12 and a cap 14 (not shown) with an alternative end-loaded RFID segment 16. The elongated rod 12 includes the RFID segment 16, a circumferential groove 18a, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the boss 22. The circumferential groove 18a is located between the end of the elongated body 20 away from the sample collection tip 24 and the RFID segment 16. The RFID segment 16 includes an RFID transition region 26, an RFID chamber 28, and an RFID terminal region 30 that receives an RFID plug 44. The RFID terminal region 30 is positioned further away from the elongated body 20 along a longitudinal axis than the RFID transition region 26, and the RFID chamber 28 is located between the RFID terminal region 30 and the RFID transition region 26. Figure 7 In the RFID segment 16, the whole is tapered or gradually thins out, and the further away it is removed from the elongated body 20, the larger the cross-section of the RFID segment 16 becomes.

[0126] Figure 8a and Figure 8bAnother embodiment of a cryopreservation device 10 with an elongated rod 12 and a cap 14 (not shown) is shown. The elongated rod 12 includes a removable attachment member 50, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The removable attachment member 50 includes an RFID segment 16, a groove 18a, and an RFID connection element 52. The elongated body 20 includes a compatible body connection element 54. The RFID connection element 52 includes a transition region 56 and an RFID locking mechanism 58. When the removable attachment member 50 is attached to the elongated body 20, the transition region 56 is away from the elongated body 20, and the RFID locking mechanism 58 is located between the transition region 56 and the elongated body 20. The RFID locking mechanism 58 includes a hollow chamber 60, wherein grooves 62a and 62b face the end of the hollow chamber 60 closest to the transition region 56. A compatible body connector 54 is positioned on the elongated body 20 away from the sample collection tip 24, and the compatible body connector 54 includes a solid object 64 and extensions 66a and 66b. Extensions 66a and 66b are positioned toward the ends of the compatible body connector 54 away from the elongated body 20. When the removable attachment 50 is attached to the elongated body 20, the solid object 64 is sized to fit within the chamber 60, and the extensions 66a and 66b are sized to fit into recesses 62a and 62b.

[0127] Figure 8c The diagram illustrates the use of Figure 8b The diagram illustrates an alternative locking mechanism for the RFID connection element 52 of the detachable attachment 50 and a compatible body connection element 54. The detachable RFID connection element 50 includes an RFID segment 16 and an RFID connection element 70. The RFID connection element 70 includes a solid object 72 with extensions 74a and 74b. When the detachable attachment 50 is attached to the elongated body 20, an RFID transition area 26 is positioned away from the elongated body 20, and the solid object 72 is located between the RFID transition area 26 and the elongated body 20. Extensions 74a and 74b are positioned toward the ends of the solid object 72 that are away from the RFID transition area 26. The compatible body locking mechanism 76 includes a hollow chamber 78 with recesses 80a and 80b. When the detachable attachment 50 is attached to the elongated body 20, the compatible body locking mechanism 76 is sized to receive the solid object 72, and the recesses 80a and 80b are sized to receive the extensions 74a and 74b.

[0128] Figure 9aAnother embodiment of a cryopreservation device 10 is illustrated, which has an elongated rod 12 and a cap 14 (not shown), and another embodiment with a top-loaded RFID segment 16. The elongated rod 12 includes a groove 18a, the RFID segment 16, an elongated body 20, a frustoconical boss 22 extending from a first end of the elongated body 20, and a sample collection tip 24 extending from a narrow end of the frustoconical boss 22. The RFID segment 16 includes an RFID transition area 26, an RFID chamber 28, and an RFID terminal area 30.

[0129] Figure 9b , Figure 9c and Figure 9d yes Figure 9a A detailed view of the RFID segment 16 of the illustrated embodiment. Figure 9b This is a cross-sectional view of the RFID tag 38 located in the RFID chamber 28. The edges of the RFID tag 38 are not visible because the locking mechanism 34 extends along the length of the RFID chamber 28 and sits on top of the side of the RFID tag 38 once the RFID tag 38 is loaded into the RFID chamber 28. Figure 9c This is a top view of the locking mechanism 34, which defines an opening 32 at the top of the RFID chamber 28. When the RFID tag 38 is fully inserted into the RFID chamber 28, the locking mechanism portions 82a and 82b extend above the RFID tag 38. The locking mechanism portions 82a and 82b are capable of holding the RFID tag 38 within the RFID chamber 28.

[0130] Although the invention has been illustrated and described herein with reference to preferred embodiments and specific examples, it will be apparent to those skilled in the art that other embodiments and examples can perform similar functions and / or achieve similar results. All such equivalent embodiments and examples are within the spirit and scope of the invention and are intended to be covered by the appended claims.

Claims

1. A cryogenic preservation device, the cryogenic preservation device comprising: a) A slender rod, the slender rod comprising an RFID segment, a slender body, and a sample collection tip; and b) A cap comprising a hollow chamber having a length sufficient to accommodate the sample collection tip. When the cap is detachably attached to the elongated rod, it can enclose the sample collection tip within the hollow cavity. Wherein, the RFID segment is far from the sample collection endpoint, and The RFID segment includes: a) An RFID chamber capable of holding RFID tags; b) An RFID opening through which the RFID tag can be placed into the RFID chamber; and c) A locking mechanism, said locking mechanism being adjacent to the periphery of the RFID opening, and The RFID opening is located at the end of the elongated rod, and the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prism-shaped profile. The RFID tag is substantially cylindrical, and the prism shape has a first longitudinal slit and a second longitudinal slit, each slit originating from the RFID opening. The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, wherein the first longitudinal slit is longer than the second longitudinal slit.

2. The cryogenic preservation device of claim 1, further comprising a truncated conical protrusion extending from a first end of the elongated body, wherein a sample collection tip extends from the truncated conical protrusion, and wherein when the cap is detachably attached to the elongated rod, the cap is capable of enclosing the sample collection tip and the truncated conical protrusion within the hollow cavity.

3. The cryogenic preservation device according to claim 1, wherein, The slender rod is made from a single piece of plastic.

4. The cryogenic preservation device according to claim 1, wherein, When the RFID tag was placed in the RFID chamber and the cryogenic storage device was placed in liquid nitrogen for 5 minutes, the RFID tag remained in the RFID chamber and the cryogenic storage device did not show any visible cracks.

5. The cryogenic preservation device according to claim 1, wherein, The RFID tag has a length ranging from 4 mm to 12 mm and a diameter ranging from 0.5 mm to 4 mm.

6. The cryogenic preservation device according to claim 1, wherein, The first longitudinal slit extends across the RFID chamber from the second longitudinal slit.

7. The cryogenic preservation device according to claim 1, wherein, The locking mechanism includes a platform that at least partially surrounds the periphery of the RFID opening.

8. The cryogenic preservation device according to claim 1, wherein, The RFID segment has a dimension of no more than 3 mm in each of the x and y directions perpendicular to the longitudinal direction.

9. The cryogenic preservation device according to claim 1, wherein, The RFID segment has a dimension of no more than 2.4 mm in each of the x and y directions perpendicular to the longitudinal direction.

10. A cryogenic preservation device, the cryogenic preservation device comprising: a) A slender rod, the slender rod comprising an RFID segment, a slender body, a truncated conical protrusion extending from a first end of the slender body, and a sample collection tip extending from the truncated conical protrusion; and b) A cap comprising a hollow chamber having a length sufficient to accommodate the sample collection tip and the truncated conical protrusion. When the cap is detachably attached to the elongated rod, it can enclose the sample collection tip within the hollow cavity. The RFID segment is located far from the sample collection endpoint. The RFID segment is capable of retaining the RFID tag, and Wherein, the dimension of the RFID segment in each of the x and y directions perpendicular to the longitudinal direction is no greater than 3 mm, and The RFID segment includes: a) An RFID chamber capable of holding the RFID tag; b) An RFID opening through which the RFID tag can be placed into the RFID chamber; and c) A locking mechanism, said locking mechanism being adjacent to the periphery of the RFID opening, and The RFID opening is located at the end of the elongated rod, and the RFID chamber comprises a substantially cylindrical chamber made of plastic with a prism-shaped profile. The RFID tag is substantially cylindrical, and the prism shape has a first longitudinal slit and a second longitudinal slit, each slit originating from the RFID opening. The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber, wherein the first longitudinal slit is longer than the second longitudinal slit.

11. The cryogenic preservation apparatus according to claim 10, wherein, The slender rod is made of a single plastic piece, and the RFID tag has a length ranging from 6 mm to 12 mm and a diameter ranging from 0.5 mm to 4 mm.

12. The cryogenic preservation device according to claim 10, wherein, The locking mechanism includes a platform that at least partially surrounds the RFID opening.

13. The cryogenic preservation device according to claim 12, wherein, The first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. The first longitudinal slit is longer than the second longitudinal slit, and the first longitudinal slit extends across the RFID chamber from the second longitudinal slit.

14. The cryogenic preservation device according to claim 10, wherein, The RFID segment has a dimension of no more than 2.4 mm in each of the x and y directions perpendicular to the longitudinal direction.

15. The cryogenic preservation device according to claim 10, wherein, The slender rod is made from a single piece of plastic.

16. The cryogenic preservation device according to claim 10, wherein, When the RFID tag was placed in the RFID chamber and the cryogenic storage device was placed in liquid nitrogen for 5 minutes, the RFID tag remained in the RFID chamber and the cryogenic storage device did not show any visible cracks.

17. A cryogenic preservation device, the cryogenic preservation device comprising: a) A slender rod, the slender rod comprising an RFID segment, a slender body, a truncated conical protrusion extending from a first end of the slender body, and a sample collection tip extending from the truncated conical protrusion; and b) A cap comprising a hollow chamber having a length sufficient to accommodate the sample collection tip and the truncated conical protrusion. When the cap is detachably attached to the elongated rod, it can enclose the sample collection tip and the truncated conical protrusion within the hollow cavity. The RFID segment is located away from the sample collection endpoint and is capable of holding the RFID tag. The RFID segment includes: a) An RFID chamber capable of holding the RFID tag; b) An RFID opening through which the RFID tag can be placed into the RFID chamber; and c) A locking mechanism, said locking mechanism being adjacent to the periphery of the RFID opening. The slender rod is made of a single plastic component. The RFID opening is located at the end of the slender rod. The RFID chamber includes a cylindrical chamber made of plastic with a prismatic shape, wherein the RFID tag is cylindrical, and wherein the prismatic shape has a first longitudinal slit and a second longitudinal slit, each slit starting from the RFID opening. Wherein, the first longitudinal slit is 50% to 95% of the length of the RFID chamber, and the second longitudinal slit is 20% to 60% of the length of the RFID chamber. The first longitudinal slit is longer than the second longitudinal slit. Wherein, the first longitudinal slit traverses the RFID chamber from the second longitudinal slit, and The locking mechanism includes a platform that at least partially surrounds the periphery of the RFID opening.

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