Convenient rice seedling stage salt-tolerant root activity monitoring device and use method thereof

Abstract

The invention relates to the technical field of rice seedling stage salt-tolerant root activity monitoring, and discloses a convenient rice seedling stage salt-tolerant root activity monitoring device which comprises a culture pond and a detection pond, a partition frame is fixedly connected to the middle of the culture pond, a water spacing ring is fixedly connected to the top end of the partition frame, a culture plate is movably placed above the water spacing ring, and a water outlet is formed in the middle of the culture pond. The two sides of the culture plate are each provided with five sets of culture openings, culture nets are fixedly installed in the middles of the culture openings, and the bottom of the culture plate is movably connected with a partition auxiliary plate. By utilizing the adsorption capacity of the surface of the rice root system, on the premise of not causing irreversible damage to the identified rice root system, monitoring and recording data of the multi-stage root vitality of the same group of rice seeds in different periods are obtained; the isolation cylinders on the isolation auxiliary plates are used for isolating the rice root systems in each group, root system entanglement is avoided, meanwhile, multiple groups of rice seed root vigor data are obtained at a time, and the monitoring efficiency is greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of rice seedling stage salt-tolerant root activity monitoring technology, and in particular to a convenient rice seedling stage salt-tolerant root activity monitoring device and a use method thereof. Background Art

[0002] Salinization of cultivated soils is becoming increasingly serious worldwide. It is one of the common abiotic stresses that affect crop growth and yield. Rice, as an important field food crop, is moderately sensitive to salt stress. Studying the salt tolerance of rice varieties and cultivating salt-tolerant varieties can improve salt tolerance while ensuring and stabilizing yields, which has important practical application value.

[0003] Rice seeds often show a variety of salt damage symptoms when subjected to salt stress. Among them, rice root activity has a strong identification correlation with the salt tolerance of rice varieties. Existing detection methods for rice roots are mostly irreversible operations, and it is difficult to monitor and record the root activity of the same group of rice seeds at different stages. There is also a lack of devices that facilitate rapid detection of root activity, and the efficiency of identifying and detecting the root activity of salt-tolerant varieties after salt stress is not high. Summary of the invention

[0004] The invention aims at the shortcomings of the existing device and method for monitoring root vitality of rice seedlings in the background technology during use.

[0005] The present invention provides the following technical solution: a convenient rice seedling salt-tolerant root activity monitoring device, comprising a culture pool and a detection pool, wherein a partition frame is fixedly connected in the middle of the culture pool, a water-isolating ring is fixedly connected to the top of the partition frame, a culture plate is movably placed above the water-isolating ring, five groups of culture ports are provided on both sides of the culture plate, a culture net is fixedly installed in the middle of the culture port, a partition auxiliary plate is movably connected to the bottom of the culture plate, the partition auxiliary plate is placed in the middle of the culture plate and the water-isolating ring, fixed buckles are movably connected on both sides of the partition auxiliary plate, card slots matching the fixed buckles are provided on both sides of the culture plate, and the partition auxiliary plate is provided at each group of culture ports. An isolation cylinder is fixedly connected to the corresponding position of the bottom, an isolation ring is fixedly connected to the top, side ear brackets are fixedly connected to the top of the front and back sides of the detection pool, a one-way tooth groove is fixedly connected to the middle of the side ear bracket, a rotating shaft is movably sleeved in the middle of the one-way tooth groove, a one-way knob is fixedly connected to the front side of the rotating shaft, and an adjusting tooth is fixedly connected to the back side of the rotating shaft. An isolation plate is placed in the detection pool, a sealing ring is fixedly installed on the top surface of the isolation plate at a position corresponding to the isolation cylinder, a cuvette slot is fixedly connected to the interior of the sealing ring and the isolation plate, an isolation frame is fixedly connected to the front and back sides of the isolation plate, and a rotating gear is provided in the middle of the isolation frame. Preferably, the isolation cylinder is connected vertically.

[0006] Preferably, a partition is provided in the middle of the detection pool, transverse slide rail grooves are provided on the top surfaces of the detection pool and the middle partition wall, and slide rails are provided on the bottom surface of the auxiliary partition plate.

[0007] Preferably, the adjusting teeth are meshed with the spiral teeth, and the rotary shaft is provided with teeth matching the one-way tooth grooves.

[0008] Preferably, the width of the adjusting tooth is greater than the width of the teeth on the rotating shaft.

[0009] A convenient method for using a salt-tolerant root activity monitoring device for rice seedlings comprises the following steps: The invention has the following beneficial effects: S1. Place conventional culture solution and culture solution with experimental set salinity on both sides of the partition in the culture tank until the liquid surface contacts the culture net; S2. After attaching the partition auxiliary plate to the bottom surface of the culture plate and fastening the buckles, place it in the culture tank in the center of the whole body; S3, placing five groups of different rice plants on five groups of culture nets respectively, and waiting for them to take root; S4. During the test, the culture plate and the auxiliary partition plate are taken out of the culture tank as a whole and placed in the test tank, so that the slide rails on the bottom surface of the auxiliary partition plate are engaged with the slide rail grooves on the top surface of the test tank; S5, injecting methylene blue solution into the water injection tank until the solution falls into the detection pool and completely submerges the rice roots, and then letting it stand for minutes; S6, opening the drain at the bottom of the detection pool to discharge the internal methylene blue solution and then closing the drain; S7, injecting clean water into the water injection tank until the clean water completely submerges the rice root system; S8, shake the culture plate and the auxiliary partition plate left and right along the slide rail to fully rinse the rice roots in the clean water, and open the drain port of the detection pool to drain; S9, repeat step SS four to five times; S10, after removing the culture plate and the auxiliary partition plate, clamp the cuvette on the cuvette clamping slot, and put the culture plate and the auxiliary partition plate back to the center position on the detection pool; S11, push the one-way knob inward until the teeth on the rotating shaft are engaged with the one-way tooth grooves, rotate the rotating shaft to pull the isolation frame and the isolation plate upward to press the sealing ring and the bottom surface of the isolation cylinder; S12, injecting calcium chloride solution into the water injection tank until the rice root system is completely submerged, and leaving it for 5-10 minutes; S13, open the drain port of the detection pool, pull the one-way knob outward to disengage the teeth on the rotating shaft from the one-way tooth groove, and then slowly reverse the one-way knob to make the isolation plate fall back; S14. After the excess solution flows out from the drain port, remove the cuvettes from the cuvette slots in order and place them in the spectrophotometer for measurement and recording; S15, placing the culture plate with the tested rice seedlings and the partition auxiliary plate back into the culture tank for further cultivation.

[0010] 1. The present invention utilizes the adsorption capacity of the surface of rice roots to obtain the root vitality of the same group of rice seeds at different stages for monitoring and recording data without causing irreversible damage to the identified rice roots; the isolation tubes on each group of separation auxiliary plates are used to isolate the roots of each group to avoid root entanglement, and the root vitality data of multiple groups of rice seeds are obtained at one time, which greatly improves the monitoring efficiency.

[0011] 2. The present invention utilizes the adsorption capacity of the surface of rice roots to soak the active roots in a methylene blue solution to adsorb methylene blue ions, so that the roots are dyed blue. After rinsing, the roots are immersed in a calcium chloride solution, and an exchange reaction occurs between calcium ions and positively charged methylene blue ions, so that the methylene blue ions on the surface of the roots enter the calcium chloride solution, thereby colorimetrically detecting the difference in the vitality of the rice roots according to the color depth of the calcium chloride solution, thereby achieving the acquisition of root vitality data without causing irreversible damage to the identified rice roots, thereby obtaining monitoring and recording data of the root vitality of the same group of rice seeds at different stages in different periods.

[0012] 3. The present invention can isolate the root systems of each group of rice by clamping a partition auxiliary plate under the culture plate and using the isolation tubes on each group of partition auxiliary plates, which can not only prevent the roots of different groups from being entangled with each other and thus affecting the accuracy of subsequent detection, but also facilitate the separation and collection of methylene blue ions adsorbed by the roots of each group and the control group during the subsequent immersion in calcium chloride solution, thereby facilitating the one-time detection of the root activity status of multiple groups of different varieties of rice, thereby greatly improving the efficiency of detection.

[0013] 4. The present invention provides a slide rail groove on the detection pool, so that in the flushing step, the roots of all experimental groups and control groups can be fully flushed in clean water by sliding the partition auxiliary plate left and right, which greatly reduces the complexity of operating multiple experimental groups, makes the operation of salt-resistant root activity detection in the rice seedling stage simpler, and greatly improves the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the culture pool of the present invention; Figure 2 It is a schematic cross-sectional view of the culture pool structure of the present invention; Figure 3 It is a schematic diagram of the overall structure of the detection pool of the present invention; Figure 4 It is a schematic diagram of the overall cross section of the detection pool of the present invention; Figure 5 It is a schematic diagram of the overall top cross-sectional view of the detection pool of the present invention; Figure 6For the present invention Figure 5 A partial enlarged schematic diagram of the structure at point A in the middle.

[0015] In the figure: 1. culture tank; 101. partition frame; 102. water-isolating ring; 2. culture plate; 201. culture port; 202. culture net; 203. water filling slot; 204. water outlet; 3. auxiliary partition plate; 301. fixing buckle; 302. isolation cylinder; 303. isolation ring; 4. detection tank; 401. side ear bracket; 402. one-way tooth groove; 5. isolation plate; 501. isolation frame; 502. sealing ring; 503. cuvette slot; 504. rotary gear; 6. adjustment gear; 601. rotary shaft; 602. one-way knob. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] See also Figure 1-2 A convenient rice seedling salt-tolerant root activity monitoring device and a method of using the same include a culture pool 1, a partition frame 101 is fixedly connected in the middle of the culture pool 1, a water-isolating ring 102 is fixedly connected to the top of the partition frame 101, a culture plate 2 is movably placed above the water-isolating ring 102, five groups of culture ports 201 are opened on both sides of the culture plate 2, a culture net 202 is fixedly installed in the middle of the culture port 201, conventional culture solution and culture solution with a test set salinity are respectively placed on both sides of the partition frame 101 in the culture pool 1 until the liquid surface contacts the culture net 202, five groups of different rice seeds to be tested are placed in the five groups of culture ports 201 for culture, a partition auxiliary plate 3 is movably connected to the bottom of the culture plate 2, and the partition auxiliary plate 3 is as long as the culture plate 2 and the partition frame 101. In the middle of the water circle 102, fixed buckles 301 are movably connected on both sides of the partition auxiliary plate 3, and slots matching the fixed buckles 301 are opened on both sides of the culture plate 2. The partition auxiliary plate 3 is fixedly connected with an isolation tube 302 at the corresponding position at the bottom of each group of culture ports 201. The isolation tube 302 passes through from top to bottom, and an isolation ring 303 is fixedly connected to the top of the isolation tube 302. The top of the isolation ring 303 is fixedly connected to the isolation ring 303. The fixed buckle 301 is rotated to make the culture plate 2 and the partition auxiliary plate 3 fit tightly and fasten. At this time, the top surface of the isolation ring 303 is squeezed to fit tightly against the culture plate 2, thereby preventing the roots grown from the rice seeds cultured out of the culture port 201 from being entangled with each other between different groups, thereby affecting the accuracy of subsequent detection and comparison.

[0018] See also Figure 3, including a detection pool 4, a partition is provided in the middle of the detection pool 4, a transverse slide groove is provided on the top surface of the detection pool 4 and the middle partition wall, and a slide rail is provided on the bottom surface of the partition auxiliary plate 3, so that the partition auxiliary plate 3 can slide left and right on the detection pool 4, so that the roots growing on the upper end can be washed in the liquid more conveniently.

[0019] See also Figure 4-6 The top of the front and back sides of the detection pool 4 are fixedly connected with side ear brackets 401, the middle of the side ear brackets 401 is fixedly connected with a one-way tooth groove 402, the middle of the one-way tooth groove 402 is movably sleeved with a rotating shaft 601, the front of the rotating shaft 601 is fixedly connected with a one-way knob 602, and the back of the rotating shaft 601 is fixedly connected with an adjusting tooth 6, an isolation plate 5 is placed in the detection pool 4, and a sealing ring 502 is fixedly installed on the top surface of the isolation plate 5 corresponding to the position of the isolation tube 302, and the inside of the sealing ring 502 is fixedly connected to the isolation plate 5 with a cuvette slot 503, the size of the cuvette slot 503 is consistent with the size of the standard cuvette, and the cuvette slot 503 can conveniently clamp the cuvette at a fixed position on the isolation plate 5, and the front and back sides of the isolation plate 5 are fixedly connected with an isolation frame 501, and the isolation frame 5 01 is provided with a spiral tooth 504 in the middle, and the adjusting tooth 6 is meshed with the spiral tooth 504. The rotating shaft 601 is provided with teeth matching the one-way tooth groove 402. The rotating shaft 601 is rotated to make the adjusting tooth 6 rotate and drive the spiral tooth 504 to move upward, so that the isolation plate 5 is moved up as a whole to the isolation frame 501 and the bottom surface of the isolation cylinder 302 is pressed together. At this time, independent closed chambers are formed between each group of isolation cylinders 302, which is convenient for independent detection of the root system in each group of isolation cylinders 302. After the detection is completed, the one-way knob 602 is pulled outward to make the teeth on the surface of the rotating shaft 601 disengage from the one-way tooth groove 402. The width of the adjusting tooth 6 is greater than the width of the teeth on the rotating shaft 601. At this time, the teeth on the rotating shaft 601 disengage from the one-way tooth groove 402, and the one-way knob 602 can be smoothly reversed to make the isolation plate 5 fall back and reset normally.

[0020] A convenient method for using a device for monitoring the salt-tolerant root activity of rice seedlings comprises the following steps: S1, placing conventional culture solution and culture solution with experimental set salinity on both sides of the partition frame 101 in the culture pool 1, respectively, until the liquid surface contacts the culture net 202; S2, attach the partition auxiliary plate 3 to the bottom surface of the culture plate 2 and fasten the buckle 301, and then place it in the culture pool 1 in the center of the whole; S3, placing five groups of different rice plants on five groups of culture nets 202 respectively, and waiting for them to take root; S4, during the detection, the culture plate 2 and the partition auxiliary plate 3 are taken out of the culture pool 1 as a whole and placed in the detection pool 4, so that the slide rail on the bottom surface of the partition auxiliary plate 3 is engaged with the slide rail groove on the top surface of the detection pool 4; S5, injecting methylene blue solution into the water injection tank 203 until the solution falls into the detection pool 4 and completely submerges the rice roots, and then letting it stand for 2 minutes; S6, open the drain port at the bottom of the detection pool 4 to discharge the internal methylene blue solution and then close the drain port; S7, injecting clean water into the water injection tank 203 until the clean water completely submerges the rice roots; S8, shake the culture plate 2 and the partition auxiliary plate 3 left and right along the slide rail to fully rinse the rice roots in the clean water, and open the drainage port of the detection pool 4 to drain; S9, repeat steps S7-S8 four to five times; S10, after removing the culture plate 2 and the auxiliary partition plate 3, clamp the cuvette on the cuvette clamping slot 503, and put the culture plate 2 and the auxiliary partition plate 3 back to the center position on the detection pool 4; S11, push the one-way knob 602 inward until the teeth on the rotating shaft 601 are engaged with the one-way tooth groove 402, rotate the rotating shaft 601 to pull the isolation frame 501 and the isolation plate 5 upward until the sealing ring 502 is pressed against the bottom surface of the isolation cylinder 302; S12, injecting calcium chloride solution into the water injection tank 203 until the rice roots are completely submerged, and leaving it for 5-10 minutes; S13, open the drain port of the detection pool 4, pull the one-way knob 602 outward to disengage the teeth on the rotating shaft 601 from the one-way tooth groove 402, and then slowly reverse the one-way knob 602 to make the isolation plate 5 fall back; S14, after the excess solution flows out from the drain port, the cuvettes on the cuvette slot 503 are removed in order and placed in a spectrophotometer for measurement and recording; S15, placing the culture plate 2 and the partition auxiliary plate 3 with the tested rice seedlings back into the culture pool 1 for further culture.

[0021] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0022] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A convenient device for monitoring the salt-tolerant root activity of rice seedlings, comprising a culture tank (1) and a detection tank (4), characterized in that: A partition frame (101) is fixedly connected in the middle of the culture pool (1), a water-isolating ring (102) is fixedly connected at the top of the partition frame (101), a culture plate (2) is movably placed above the water-isolating ring (102), five groups of culture ports (201) are provided on both sides of the culture plate (2), a culture net (202) is fixedly installed in the middle of the culture ports (201), a partition auxiliary plate (3) is movably connected at the bottom of the culture plate (2), the partition auxiliary plate (3) is located in the middle of the culture plate (2) and the water-isolating ring (102), fixed buckles (301) are movably connected on both sides of the partition auxiliary plate (3), card slots matching the fixed buckles (301) are provided on both sides of the culture plate (2), an isolation cylinder (302) is fixedly connected to the partition auxiliary plate (3) at a corresponding position at the bottom of each group of culture ports (201), and the top of the isolation cylinder (302) is fixedly connected to the isolation cylinder (302). The detection pool (4) is fixedly connected with an isolation ring (303); the top ends of the front and back sides of the detection pool (4) are fixedly connected with side ear brackets (401); the middle of the side ear brackets (401) is fixedly connected with a one-way tooth groove (402); the middle of the one-way tooth groove (402) is movably sleeved with a rotating shaft (601); the front side of the rotating shaft (601) is fixedly connected with a one-way knob (602); the back side of the rotating shaft (601) is fixedly connected with an adjusting tooth (6); an isolation plate (5) is placed in the detection pool (4); a sealing ring (502) is fixedly installed on the top surface of the isolation plate (5) at a position corresponding to the isolation cylinder (302); the inside of the sealing ring (502) is fixedly connected with a cuvette slot (503) and the isolation plate (5); the front and back sides of the isolation plate (5) are fixedly connected with an isolation frame (501); and the middle of the isolation frame (501) is provided with a rotating tooth (504).

2. A convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that: The isolation cylinder (302) is connected vertically, and a drainage outlet is provided at the bottom of the detection pool (4).

3. A convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that: A partition is provided in the middle of the detection pool (4), transverse slide rail grooves are provided on the top surfaces of the detection pool (4) and the middle partition wall, and slide rails are provided on the bottom surface of the partition auxiliary plate (3).

4. A convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that: The adjusting tooth (6) meshes with the rotating tooth (504), and the rotating shaft (601) is provided with a meshing tooth that matches the one-way tooth groove (402).

5. A convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that: The width of the adjusting teeth (6) is greater than the width of the teeth on the rotating shaft (601).

6. The method for using the convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that: The steps include: S1. Place conventional culture solution and culture solution with a test set salinity on both sides of the partition frame (101) in the culture pool (1) until the liquid surface contacts the culture net (202); S2, attaching the partition auxiliary plate (3) to the bottom surface of the culture plate (2) and fastening the buckle (301) to fix it, and then placing it in the culture pool (1) in the center of the whole; S3, placing five groups of different rice plants on five groups of culture nets (202) respectively, and waiting for them to take root; S4, during the test, the culture plate (2) and the partition auxiliary plate (3) are taken out of the culture tank (1) as a whole and placed in the test tank (4), so that the slide rail on the bottom surface of the partition auxiliary plate (3) is engaged with the slide rail groove on the top surface of the test tank (4); S5, injecting methylene blue solution into the water injection tank (203) until the solution falls into the detection pool (4) and completely submerges the rice roots, and leaving it to stand for 2 minutes; S6, opening the drain outlet at the bottom of the detection pool (4) to discharge the methylene blue solution inside, and then closing the drain outlet; S7, injecting clean water into the water injection tank (203) until the clean water completely submerges the rice root system; S8, shaking the culture plate (2) and the auxiliary partition plate (3) left and right along the slide rail so that the rice roots are fully rinsed in the clean water, and opening the drainage port of the detection pool (4) to drain; S9, repeat steps S7-S8 four to five times; S10, after removing the culture plate (2) and the auxiliary partition plate (3), clamp the cuvette on the cuvette clamping slot (503), and put the culture plate (2) and the auxiliary partition plate (3) back to the center position on the detection pool (4); S11, pushing the one-way knob (602) inward until the teeth on the rotating shaft (601) mesh with the one-way tooth groove (402), rotating the rotating shaft (601) to pull the isolation frame (501) and the isolation plate (5) upward until the sealing ring (502) is pressed against the bottom surface of the isolation cylinder (302); S12, injecting calcium chloride solution into the water injection tank (203) until the rice root system is completely submerged, and leaving it to stand for 5-10 minutes; S13, opening the drain port of the detection pool (4), pulling the one-way knob (602) outward to disengage the teeth on the rotating shaft (601) from the one-way tooth groove (402), and then slowly reversing the one-way knob (602) to cause the isolation plate (5) to fall back; S14, after the excess solution flows out from the drain port, the cuvettes on the cuvette holder (503) are removed in order and placed in a spectrophotometer for measurement and recording; S15, placing the culture plate (2) with the tested rice seedlings and the partition auxiliary plate (3) back into the culture pool (1) for continued cultivation.

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