A cleansing composition and its application in a cleansing cream

Abstract

This invention relates to a skin-cleansing composition and its application in a cleansing cream, belonging to the field of daily chemical technology. The skin-cleansing composition comprises the following raw materials: plant fermentation broth, artemisia argyi extract, silk amino acids, cetyl ethylhexanoate, PEG-20 glyceryl triisostearate, and soapberry seed oil. The preparation method of the plant fermentation broth includes: S1, pretreating some of the raw materials; S2, activating and culturing the bacterial strains; S3, adjusting the pH of mixture A, sterilizing under controlled temperature, cooling, adding mannanase for enzymatic hydrolysis under controlled temperature, inactivating the enzyme after hydrolysis to obtain mixture B, cooling, adding Lactobacillus acidophilus fermentation seed broth and Streptococcus thermophilus fermentation seed broth for static fermentation under controlled temperature, sterilizing under controlled temperature after fermentation, centrifuging at controlled speed, and taking the supernatant for microfiltration through a 0.22μm membrane to obtain the plant fermentation broth. The skin-cleansing composition provided by this invention has synergistic effects among its raw materials, enabling the finished product to effectively cleanse the skin while also providing moisturizing and whitening effects.

Description

Technical Field

[0001] This invention belongs to the field of daily chemical technology, specifically relating to a skin-cleansing composition and its application in a cleansing cream. Background Technology

[0002] With the fast pace of modern life and increasing environmental pollution, skin problems have become a growing concern. As the largest organ in the human body, the skin not only protects internal organs but also plays a vital role in regulating body temperature and sensing external stimuli. However, long-term exposure to ultraviolet radiation, air pollution, and unhealthy lifestyle habits can lead to problems such as excessive oil secretion, dullness, and dryness. The main function of cleansing cream is to deeply cleanse the skin, removing dirt, oil, and makeup residue from the skin's surface. While using cleansing cream after makeup application can effectively remove makeup, it is also suitable for daily skin cleansing. It can penetrate deep into pores to remove dirt and excess oil, helping to maintain normal skin metabolism and respiration. At the same time, some high-quality cleansing creams also contain moisturizing and nourishing ingredients, providing essential nutrients and moisture to the skin while cleansing, maintaining its healthy state. Currently, there are very few combinations on the market that offer multiple functions simultaneously. Traditional cleansing creams primarily focus on cleansing, using oil components to dissolve oil and makeup on the skin's surface, but often fail to address whitening and moisturizing simultaneously.

[0003] Therefore, developing a composition that integrates cleansing, whitening, and moisturizing, and applying it to cleansing cream products to meet consumers' needs for comprehensive skin care, is of great practical significance. Summary of the Invention

[0004] The purpose of this invention is to provide a skin-cleansing composition and its application in a cleansing cream, which solves the problem in the prior art that the skin-cleansing composition also has moisturizing and whitening effects while cleansing the skin*.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] A skin-cleansing composition, wherein the skin-cleansing composition specifically comprises the following raw materials in parts by weight:

[0007] 4-6 parts by weight of plant fermentation broth, 7-10 parts by weight of Artemisia argyi extract, 15-22 parts by weight of silk amino acids, 44-52 parts by weight of cetyl ethylhexanoate, 20-26 parts by weight of PEG-20 glyceryl triisostearate, and 50-60 parts by weight of solvent.

[0008] The preparation method of the plant fermentation broth includes the following steps:

[0009] S1. Pre-treat some of the raw materials to obtain mixture A;

[0010] S2. Activate and culture the bacterial strains to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid;

[0011] S3. Adjust the pH of mixture A, sterilize under controlled temperature, cool, add mannanase for enzymatic hydrolysis under controlled temperature, inactivate the enzyme after hydrolysis to obtain mixture B, cool, add Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid for static fermentation under controlled temperature, sterilize under controlled temperature after fermentation, centrifuge at controlled speed, take the supernatant and filter through a 0.22μm membrane to obtain plant fermentation broth.

[0012] Furthermore, the chrysanthemum extract is obtained by using the whole herb of Tanacetum vulgare (a herbaceous plant of the genus Tanacetum in the family Asteraceae) as raw material, with deionized water as solvent, and by conventional reflux extraction at a material-to-liquid ratio of 1:15. The chrysanthemum extract contains artemisinic acid, flavonoids and gallic acid, which can promote the production of hyaluronic acid synthase, thereby replenishing moisture to the skin.

[0013] The silk amino acids (SILK AMINO ACIDS), CAS: 96692-41-4, were purchased from Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.; cetyl ethylhexanoate, CAS: 59130-69-7, were purchased from Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.; and the PEG-20 glycerol triisostearate was purchased from Shenzhen Xingkaiyue Biotechnology Co., Ltd.

[0014] As a preferred technical solution of the present invention, step S1 specifically includes: air-drying and crushing matsutake mushrooms and dodder seeds, sieving them to obtain matsutake mushroom powder and dodder seed powder, mixing matsutake mushroom powder, dodder seed powder and deionized water evenly, sterilizing under controlled temperature to obtain mixture A for later use.

[0015] Tricholoma matsutake, a fungus belonging to the genus Tricholomataceae in the family Tricholomataceae, is rich in polysaccharides, polypeptides, and other chemical components. It can stably and effectively inhibit tyrosinase activity, achieving whitening and freckle-removing effects. Cuscuta chinensis Lam., the dried mature seed of an annual parasitic herb belonging to the genus Cuscuta in the family Convolvulaceae, mainly contains polysaccharides, flavonoids, polyphenols, and sterols. It can significantly reduce the production of melanin in B16 cells.

[0016] Furthermore, the mass ratio of the matsutake mushroom powder to the dodder seed powder is 2-3:1.5-3; the amount of deionized water added is 100-110 times the total mass of the matsutake mushroom powder and the dodder seed powder.

[0017] Furthermore, the sieving is performed through a 50-60 mesh sieve; the temperature for temperature-controlled sterilization is 121℃, and the time is 20 minutes.

[0018] As a preferred embodiment of the present invention, step S2 specifically includes: inoculating Lactobacillus acidophilus and Streptococcus thermophilus into MRS liquid culture medium for a single temperature-controlled culture to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus; inoculating the activated Lactobacillus acidophilus and activated Streptococcus thermophilus into MRS liquid culture medium at an inoculation amount of 3-5% by volume, and then incubating them a second time at a controlled temperature to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid.

[0019] Furthermore, the Lactobacillus acidophilus (product number xyh001) was purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd.; and the Streptococcus thermophilus was purchased from Guangdong Hongyou Biotechnology Co., Ltd.

[0020] Further, the first temperature-controlled culture consists of static culture at 37-42℃ for 32-40 hours; the second temperature-controlled culture consists of static culture at 37-42℃ until the concentration of the Lactobacillus acidophilus fermentation seed liquid reaches 2-2.1×10⁻⁶. 8 CFU / mL, the concentration of the thermophilic streptococcus fermentation seed culture is 1.8-2×10⁻⁶. 8 CFU / mL.

[0021] As a preferred embodiment of the present invention, in step S3, the mass ratio of mixture A and mannanase is 1:0.002-0.003; the mass ratio of mixture B, Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid is 1:0.003-0.005:0.002-0.003.

[0022] Furthermore, the enzymatic activity of the mannanase includes 1 × 10⁻⁶. 4 U / g.

[0023] As a preferred embodiment of the present invention, in step S3, the pH of the mixture A is adjusted to 7.0-7.5 using a 0.1 mol / L sodium hydroxide solution; the temperature for temperature-controlled sterilization is 121°C for 15 min; the cooling is performed to room temperature; the temperature for temperature-controlled enzymatic hydrolysis is 55-60°C for 2-3 h; the enzyme inactivation is performed by boiling water bath for 20-30 min; the temperature for temperature-controlled static fermentation is 37-40°C for 48 h; and the centrifugation speed is 8000-9000 r / min for 20-30 min.

[0024] In a preferred embodiment of the present invention, the solvent includes ethanol.

[0025] As a preferred embodiment of the present invention, the skin-cleansing composition further includes Sapindus mukorossi seed oil.

[0026] Furthermore, the amount of Sapindus mukorossi seed oil is 1-1.6 parts by weight.

[0027] Furthermore, Sapindus mukorossi seed oil is obtained by using the dried seeds of Sapindus mukorossi (SCHLEICHERA TRIJUGA) as raw material, with n-hexane as solvent, and by conventional Soxhlet reflux extraction at a material-to-liquid ratio of 1:20. Sapindus mukorossi seed oil mainly contains oleic acid, cis-11-eicosenoic acid, linoleic acid, arachidic acid and other components, and has excellent cleansing and moisturizing effects on the skin.

[0028] A method for preparing a skin-cleansing composition includes the following steps: mixing cetyl ethylhexanoate, PEG-20 glyceryl triisostearate and solvent under controlled temperature until homogeneous, then adding the remaining raw materials and mixing and stirring until homogeneous to obtain the finished skin-cleansing composition.

[0029] As a preferred embodiment of the present invention, the temperature for temperature control is 30-35℃.

[0030] An application of a cleansing composition, wherein the cleansing composition is added to a skin care product at a mass percentage of 3-5%.

[0031] As a preferred embodiment of the present invention, the skin care products include, but are not limited to, cleansing creams, facial cleansers, and cleansing pastes.

[0032] The beneficial effects of this invention are:

[0033] (1) This invention uses matsutake mushrooms and dodder seeds as raw materials to inhibit tyrosinase activity and reduce melanin production in B16 cells, thereby synergistically giving the resulting composition a certain whitening effect. At the same time, based on this, this invention first uses mannanase for enzymatic hydrolysis and then uses Lactobacillus acidophilus and Streptococcus thermophilus to co-ferment matsutake mushrooms and dodder seeds. Mannanase increases the polysaccharide content of the system, thereby playing a better auxiliary role in the process of inhibiting tyrosinase in the composition. Streptococcus thermophilus degrades the macromolecules in the system into small molecules, and Lactobacillus acidophilus releases the polypeptide components in the system. Therefore, under the enzymatic hydrolysis and fermentation process of this invention, matsutake mushrooms and dodder seeds can greatly assist in enhancing the whitening effect of the finished composition.

[0034] (2) The present invention combines cetyl ethylhexanoate and PEG-20 glyceryl triisostearate, and starts from two different aspects of oil-like solubility and emulsification to make the finished product of the composition highly effective in cleansing. In addition, the soapberry seed oil added in the present invention, as a natural plant oil, also has a certain cleansing effect, which can assist cetyl ethylhexanoate in strengthening its oil-dissolving effect and thus enhance the cleansing function of the finished product of the composition.

[0035] (3) This invention incorporates artemisia extract containing artemisinic acid, flavonoids, and gallic acid to promote the production of hyaluronic acid synthase, thereby replenishing the skin with moisture; it also incorporates soapberry seed oil, which mainly contains oleic acid, cis-11-eicosenoic acid, and linoleic acid, to moisturize the skin; and it incorporates silk amino acids, which have highly hydrophilic properties, to effectively remain on the skin to form a protective and moisturizing film and lock in skin moisture. Therefore, the artemisia extract, soapberry seed oil, and silk amino acids incorporated in this invention can work synergistically to replenish moisture, moisturize the skin, and lock in moisture, resulting in a composition with outstanding moisturizing effect.

[0036] (4) The skin-cleansing composition provided by the present invention can effectively cleanse the skin while fully hydrating and locking in moisture, thereby maintaining the skin's hydration and preventing dryness and tightness. In addition, the composition also has excellent whitening effects, which can effectively improve skin tone and enhance the overall health and beauty of the skin. Detailed Implementation

[0037] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0038] The chrysanthemum extract used in this embodiment of the invention was obtained by conventional reflux extraction of the whole herb *Tanacetum vulgare* (a herbaceous plant of the genus *Tanacetum* in the family Asteraceae) using deionized water as solvent at a material-to-liquid ratio of 1:15. The silk amino acids (SILK AMINO ACIDS) and cetyl ethylhexanoate used were purchased from Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.; the PEG-20 glycerol triisostearate used was purchased from Shenzhen Xingkaiyue Biotechnology Co., Ltd.; the *Lactobacillus acidophilus* (catalog number xyh001) used was purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd.; the *Streptococcus thermophilus* used was purchased from Guangdong Hongyou Biotechnology Co., Ltd.; the mannanase used had an enzyme activity of 1×10⁴ U / g; and the soapberry seed oil used was obtained by conventional Soxhlet reflux extraction of dried seeds of *Schisandra trichuga* (a type of soapberry) using n-hexane as solvent at a material-to-liquid ratio of 1:20. Further details will not be elaborated upon hereafter.

[0039] Example 1

[0040] The method for preparing plant fermentation broth includes the following steps:

[0041] S1. Air dry and crush the matsutake mushrooms and dodder seeds, and pass them through a 50-mesh sieve to obtain matsutake mushroom powder and dodder seed powder. Mix the matsutake mushroom powder, dodder seed powder and deionized water evenly, and sterilize at 121℃ for 20 minutes to obtain mixture A, which is ready for use.

[0042] The mass ratio of matsutake mushroom powder to dodder seed powder is 2.5:1.5; the amount of deionized water added is 105 times the total mass of matsutake mushroom powder and dodder seed powder.

[0043] S2. Lactobacillus acidophilus and Streptococcus thermophilus were separately inoculated into MRS liquid medium and cultured at 37°C for 36 hours to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus. The activated Lactobacillus acidophilus and activated Streptococcus thermophilus were then inoculated into MRS liquid medium at a volume fraction of 3% and cultured a second time at 37°C until the Lactobacillus acidophilus fermentation seed culture concentration reached 2 × 10⁻⁶. 8 CFU / mL, the concentration of the thermophilic streptococcus fermentation seed culture was 1.8 × 10⁻⁶. 8 CFU / mL;

[0044] S3. Adjust the pH of mixture A to 7.0 using 0.1 mol / L sodium hydroxide solution, sterilize at 121℃ for 15 min, cool to room temperature, add mannanase and hydrolyze at 55℃ for 2 h, inactivate the enzyme in a boiling water bath for 25 min to obtain mixture B, cool, add Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid, and let it ferment at 37℃ for 48 h, sterilize at 121℃ for 15 min after fermentation, centrifuge at 9000 r / min for 30 min, take the supernatant and filter it through a 0.22 μm membrane to obtain plant fermentation broth;

[0045] The mass ratio of mixture A to mannanase is 1:0.0025; the mass ratio of mixture B, Lactobacillus acidophilus fermentation seed liquid, and Streptococcus thermophilus fermentation seed liquid is 1:0.003:0.0025.

[0046] Example 2

[0047] The method for preparing plant fermentation broth includes the following steps:

[0048] S1. Air-dry and crush the matsutake mushrooms and dodder seeds, and pass them through a 60-mesh sieve to obtain matsutake mushroom powder and dodder seed powder. Mix the matsutake mushroom powder, dodder seed powder and deionized water evenly, and sterilize at 121℃ for 20 minutes to obtain mixture A, which is ready for use.

[0049] The mass ratio of matsutake mushroom powder to dodder seed powder is 3:2.2; the amount of deionized water added is 100 times the total mass of matsutake mushroom powder and dodder seed powder.

[0050] S2. Lactobacillus acidophilus and Streptococcus thermophilus were separately inoculated into MRS liquid medium and cultured at 42℃ for 32 hours to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus. The activated Lactobacillus acidophilus and activated Streptococcus thermophilus were then inoculated into MRS liquid medium at a volume fraction of 5% and cultured a second time at 42℃ until the concentration of the Lactobacillus acidophilus fermentation seed culture reached 2.1 × 10⁻⁶. 8 CFU / mL, the concentration of Streptococcus thermophilus fermentation seed culture was 2×10⁻⁶. 8 CFU / mL;

[0051] S3. Adjust the pH of mixture A to 7.5 using 0.1 mol / L sodium hydroxide solution, sterilize at 121℃ for 15 min, cool to room temperature, add mannanase and hydrolyze at 60℃ for 2.5 h, inactivate enzyme in boiling water bath for 20 min to obtain mixture B, cool, add Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid, and let ferment at 40℃ for 48 h, sterilize at 121℃ for 15 min after fermentation, centrifuge at 8000 r / min for 20 min, take the supernatant and filter through a 0.22 μm membrane to obtain plant fermentation broth;

[0052] The mass ratio of mixture A to mannanase is 1:0.003; the mass ratio of mixture B, Lactobacillus acidophilus fermentation seed liquid, and Streptococcus thermophilus fermentation seed liquid is 1:0.004:0.003.

[0053] Example 3

[0054] The method for preparing plant fermentation broth includes the following steps:

[0055] S1. Air dry and crush the matsutake mushrooms and dodder seeds, and pass them through a 50-mesh sieve to obtain matsutake mushroom powder and dodder seed powder. Mix the matsutake mushroom powder, dodder seed powder and deionized water evenly, and sterilize at 121℃ for 20 minutes to obtain mixture A, which is ready for use.

[0056] The mass ratio of matsutake mushroom powder to dodder seed powder is 2:3; the amount of deionized water added is 110 times the total mass of matsutake mushroom powder and dodder seed powder.

[0057] S2. Lactobacillus acidophilus and Streptococcus thermophilus were separately inoculated into MRS liquid medium and cultured at 40℃ for 40 hours to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus. The activated Lactobacillus acidophilus and activated Streptococcus thermophilus were then inoculated into MRS liquid medium at a volume fraction of 4% and cultured a second time at 40℃ until the concentration of the Lactobacillus acidophilus fermentation seed culture reached 2×10⁻⁶. 8 CFU / mL, the concentration of Streptococcus thermophilus fermentation seed culture was 2×10⁻⁶. 8 CFU / mL;

[0058] S3. Adjust the pH of mixture A to 7.3 using 0.1 mol / L sodium hydroxide solution, sterilize at 121℃ for 15 min, cool to room temperature, add mannanase and hydrolyze at 57.5℃ for 3 h, inactivate the enzyme in a boiling water bath for 30 min to obtain mixture B, cool, add Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid, and let it ferment at 38.5℃ for 48 h, sterilize at 121℃ for 15 min after fermentation, centrifuge at 8500 r / min for 25 min, take the supernatant and filter through a 0.22 μm membrane to obtain plant fermentation broth;

[0059] The mass ratio of mixture A to mannanase is 1:0.002; the mass ratio of mixture B, Lactobacillus acidophilus fermentation seed liquid, and Streptococcus thermophilus fermentation seed liquid is 1:0.005:0.002.

[0060] Comparative Example 1

[0061] Compared with Example 2, the difference is that in step S1 of Comparative Example 1, matsutake mushroom powder is not added, but dodder powder of the same weight as matsutake mushroom powder is used instead, while the other operation steps and parameters are the same.

[0062] Comparative Example 2

[0063] Compared with Example 2, the difference is that in step S1 of Comparative Example 2, do not add dodder powder, but use matsutake mushroom powder of the same mass as dodder powder instead, while the other operation steps and parameters are the same.

[0064] Comparative Example 3

[0065] Compared with Example 2, the difference is that mannanase is not added in step S3 of Comparative Example 3, while the other operation steps and parameters are the same.

[0066] Comparative Example 4

[0067] Compared with Example 2, the difference is that Lactobacillus acidophilus fermentation seed liquid was not added in step S3 of Comparative Example 4, while the other operation steps and parameters were the same.

[0068] Comparative Example 5

[0069] Compared with Example 2, the difference is that in step S3 of Comparative Example 5, no thermophilic streptococcal fermentation seed liquid was added, while the other operation steps and parameters were the same.

[0070] Examples 4-9

[0071] A method for preparing a skin-cleansing composition specifically includes the following steps:

[0072] Cetyl ethylhexanoate, PEG-20 glyceryl triisostearate and ethanol are mixed evenly at a controlled temperature of 32.5℃. Then the remaining raw materials are added and mixed and stirred evenly to obtain the skin cleansing composition.

[0073] The composition and weight parts of each raw material in Examples 4-9 are shown in Table 1:

[0074] Table 1

[0075]

[0076] Example 10

[0077] The skin-cleansing composition obtained in Example 9 was added to the cleansing cream at a mass percentage of 3%.

[0078] Example 11

[0079] The skin-cleansing composition obtained in Example 8 was added to the facial cleanser at a mass percentage of 5%.

[0080] Example 12

[0081] The skin-cleansing composition obtained in Example 7 was added to the cleansing cream at a mass percentage of 4%.

[0082] Comparative Examples 6-10

[0083] Compared with Example 9, the difference between Comparative Examples 6-10 is that the plant fermentation liquid prepared in Comparative Examples 1-5 is used instead of the plant fermentation liquid prepared in Example 2 of Example 9, while the other operation steps and parameters are the same.

[0084] Comparative Example 11

[0085] Compared with Example 9, the difference is that cetyl ethylhexanoate was not added in Comparative Example 11, but was replaced with half the weight of PEG-20 glycerol triisostearate and half the weight of cetyl ethylhexanoate in Sapindus mukorossi seed oil. All other operating steps and parameters were the same.

[0086] Comparative Example 12

[0087] Compared with Example 9, the difference is that PEG-20 glycerol triisostearate was not added in Comparative Example 12, but was replaced with half the weight of cetyl ethylhexanoate of PEG-20 glycerol triisostearate and half the weight of Sapindus mukorossi seed oil. All other operating steps and parameters were the same.

[0088] Comparative Example 13

[0089] Compared with Example 9, the difference is that Sapindus mukorossi seed oil was not added in Comparative Example 13, but was replaced with half the weight of Sapindus mukorossi seed oil in cetyl ethylhexanoate and half the weight of Sapindus mukorossi seed oil in PEG-20 glyceryl triisostearate. All other operating steps and parameters were the same.

[0090] Comparative Example 14

[0091] Compared with Example 9, the difference is that Artemisia argyi extract was not added in Comparative Example 14, but instead, half the weight of Sapindus mukorossi seed oil and half the weight of Artemisia argyi extract were used as substitutes. All other operating steps and parameters were the same.

[0092] Comparative Example 15

[0093] Compared with Example 9, the difference is that Sapindus mukorossi seed oil was not added in Comparative Example 15, but was replaced with half the weight of Sapindus mukorossi seed oil containing Artemisia argyi extract and half the weight of Sapindus mukorossi seed oil containing silk amino acids. All other operating steps and parameters were the same.

[0094] Comparative Example 16

[0095] Compared with Example 9, the difference is that silk amino acids were not added in Comparative Example 16, but were replaced with half the weight of Artemisia argyi extract and half the weight of Sapindus mukorossi seed oil containing silk amino acids. All other operating steps and parameters were the same.

[0096] Test Example 1

[0097] The tyrosinase inhibition ability of the skin-cleansing compositions prepared in Examples 6-9 and Comparative Examples 6-10 was tested by a tyrosinase inhibition experiment, thereby reflecting the whitening effect.

[0098] Solution preparation: Prepare a 5 U / L enzyme solution, aliquot into 1.5 mL EP tubes, and store in liquid nitrogen. Before use, dilute with PBS to prepare a 0.5 U / L tyrosinase solution, and prepare fresh each time. Accurately weigh L-tyrosine and L-DOPA, add an appropriate amount of PBS solution, and sonicate at room temperature for 30 minutes to obtain a substrate solution with a concentration of 3.0 mmol / L; accurately weigh 1 g of kojic acid (positive control) solution, dissolve in DMSO, and dilute with an appropriate amount of PBS solution to a final DMSO content of 0.5%; weigh 1 g of the skin-cleansing composition products obtained in Examples 6-9 and Comparative Examples 6-10, dissolve in DMSO, and dilute with an appropriate amount of PBS solution to a final DMSO content of 0.5%.

[0099] Tyrosinase activity inhibition rate determination:

[0100] Take 40 μL of sample, 10 μL of PBS, and 10 μL of enzyme solution sequentially, mix them, and incubate at 37°C for 10 min. Add 50 μL of substrate solution and incubate for 25 min. Measure the absorbance at 475 nm using a multi-functional microplate reader. Calculate the inhibition rate based on the absorbance value using the following formula:

[0101] Inhibition rate (%) = [blank - (assay - positive control)] / blank x 100%;

[0102] The final results are shown in Table 2.

[0103] Table 2

[0104]

[0105] As can be seen from Table 2, the finished product of the composition obtained by the present invention has an excellent tyrosinase activity inhibition rate, that is, the finished product of the composition obtained by the present invention has excellent whitening effect.

[0106] Test Example 2

[0107] Skin cleansing effect test:

[0108] Number of participants: 24 people per group, with males and females randomly assigned to each group, all meeting the participant criteria;

[0109] Age: 22-45 years old;

[0110] Test samples: Skin cleansing compositions prepared in Examples 6-9 and Comparative Examples 11-13.

[0111] Test method: Clean and dry the normal skin on the flexor side of the subject's forearm. Apply the same commercially available foundation to the forearm at a concentration of 1.25 mg / cm². 2 After drying for 30 minutes, apply 0.1g of the test sample to each area where foundation has been applied, massage in circular motions for 25 seconds, then rinse with water and pat dry. The skin brightness (L) of the test areas before makeup application, after makeup application, and after cleansing is measured to calculate the cleansing ability. The calculation formula is shown below:

[0112] Formula: S=(L 净肤后 -L 上妆后 ) / (L 上妆前 -L 上妆后 );

[0113] Where S represents skin cleansing ability and L represents skin brightness after cleansing;

[0114] The final result is the average of each group, and the specific test results are shown in Table 3.

[0115] Table 3

[0116]

[0117] As can be seen from Table 3, the finished product of the composition prepared by the present invention has outstanding skin-cleansing ability.

[0118] Test Example 3

[0119] Hydration and moisturizing test: Male and female subjects aged 25-40 years were randomly selected and randomly divided into 7 groups of 12 subjects each, with half males and half females. Each group of subjects used a cleansing cream prepared by adding the compositions obtained in Examples 6-9 and Comparative Examples 14-16 to the same cleansing cream base (the amount of composition added to the cleansing cream was 3%).

[0120] The method of use is as follows: Apply 1mL of the cleansing cream evenly to the cheek area of ​​the face and massage in circular motions for 25 seconds, then rinse with water and pat dry. Use a Corneomenter CM 825 skin moisture content meter to measure the skin moisture content (MMV) of the subjects before use and 1 hour and 2 hours after use. Calculate the skin moisture content growth rate according to the following formula and record the average value:

[0121] Moisture content growth rate (%) = (MMV) t -MMV0) / MMV0×100%;

[0122] In the formula, MMV0 represents the skin's moisture content before use, and MMV t This represents the skin moisture content at time t after use.

[0123] The results are shown in Table 4.

[0124] Table 4

[0125]

[0126] As can be seen from Table 4, the finished product of the composition prepared by the present invention has an outstanding moisturizing effect.

[0127] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0128] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. A skin-cleansing composition, characterized in that, The skin-cleansing composition specifically comprises the following raw materials in parts by weight: 4-6 parts by weight of plant fermentation broth, 7-10 parts by weight of Artemisia argyi extract, 15-22 parts by weight of silk amino acids, 44-52 parts by weight of cetyl ethylhexanoate, 20-26 parts by weight of PEG-20 glyceryl triisostearate, 1-1.6 parts by weight of Sapindus mukorossi seed oil, and 50-60 parts by weight of ethanol. The preparation method of the plant fermentation broth includes the following steps: S1. Air-dry and crush the matsutake mushrooms and dodder seeds, and sieve them to obtain matsutake mushroom powder and dodder seed powder. Mix the matsutake mushroom powder, dodder seed powder and deionized water evenly, and sterilize under controlled temperature to obtain mixture A for later use. S2. Activate and culture the bacterial strains to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid; S3. Adjust the pH of mixture A, sterilize under controlled temperature, cool, add mannanase for enzymatic hydrolysis under controlled temperature, inactivate the enzyme after hydrolysis to obtain mixture B, cool, add Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid for static fermentation under controlled temperature, sterilize under controlled temperature after fermentation, centrifuge at controlled speed, take the supernatant and filter through a 0.22μm membrane to obtain plant fermentation broth.

2. The skin-cleansing composition according to claim 1, characterized in that, The mass ratio of matsutake mushroom powder to dodder seed powder in step S1 is 2-3:1.5-3; the amount of deionized water added is 100-110 times the total mass of matsutake mushroom powder and dodder seed powder.

3. The skin-cleansing composition according to claim 1, characterized in that, Step S2 specifically includes: inoculating Lactobacillus acidophilus and Streptococcus thermophilus into MRS liquid culture medium and culturing them at a controlled temperature once to obtain activated Lactobacillus acidophilus and activated Streptococcus thermophilus; inoculating the activated Lactobacillus acidophilus and activated Streptococcus thermophilus into MRS liquid culture medium at an inoculation amount of 3-5% by volume and culturing them at a controlled temperature twice to obtain Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid.

4. The skin-cleansing composition according to claim 1, characterized in that, In step S3, the mass ratio of mixture A and mannanase is 1:0.002-0.003; the mass ratio of mixture B, Lactobacillus acidophilus fermentation seed liquid and Streptococcus thermophilus fermentation seed liquid is 1:0.003-0.005:0.002-0.

003.

5. The skin-cleansing composition according to claim 3, characterized in that, The first temperature-controlled culture consists of static incubation at 37-42℃ for 32-40 hours; the second temperature-controlled culture consists of static incubation at 37-42℃ until the concentration of the Lactobacillus acidophilus fermentation seed liquid reaches 2-2.1×10⁻⁶. 8 CFU / mL, the concentration of the thermophilic streptococcus fermentation seed culture is 1.8-2×10⁻⁶. 8 CFU / mL.

6. The skin-cleansing composition according to claim 1, characterized in that, The mannanase has an enzyme activity of 1×10⁻⁶. 4 U / g.

7. The application of a skin-cleansing composition according to any one of claims 1-6, characterized in that, The cleansing composition is added to skin care products at a weight percentage of 3-5%.

Images