Traditional Chinese medicine preparation for preventing and treating myelosuppression after chemotherapy

Abstract

The invention provides a traditional Chinese medicine preparation for preventing and treating myelosuppression after chemotherapy, and relates to the field of traditional Chinese medicine pharmacy, the traditional Chinese medicine preparation is prepared from 1-15 parts of deer-horn glue, 1-10 parts of astragalus membranaceus, 1-10 parts of cassia twig, 1-10 parts of pseudo-ginseng, 1-10 parts of immature bitter orange, 1-10 parts of rhizoma anemarrhenae and 1-10 parts of golden cypress, and the traditional Chinese medicine preparation has the effects of invigorating qi and blood, promoting blood circulation to remove blood stasis, relieving swelling and pain and the like. The traditional Chinese medicine preparation disclosed by the invention can reverse myelosuppression caused by 5-FU chemotherapy by improving the peripheral hemogram of a chemotherapy mouse and pathological injury of bone marrow. In addition, the traditional Chinese medicine preparation can improve the 5-FU chemotherapy effect by increasing the 5-FU tumor inhibition rate, inhibiting tumor weight and tumor volume and promoting tumor tissue necrosis. The traditional Chinese medicine preparation disclosed by the invention is combined with a chemotherapeutic drug 5-FU for application, has certain effects of enhancing effects and reducing toxicity, and is expected to be developed into a drug for preventing and treating myelosuppression after chemotherapy.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine pharmaceutical preparations, and in particular to a traditional Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy. Background Art

[0002] Chemotherapy is the main treatment for advanced tumors and other treatment options that are not suitable for surgery. It has important clinical value in the comprehensive treatment of advanced tumors. Bone marrow suppression caused by chemotherapy drugs will not only affect the entire chemotherapy process, but also weaken the patient's immune function, further induce infection, sepsis and other serious complications, making it impossible for patients to continue treatment, seriously affecting the patient's prognosis and quality of life. In recent years, the clinical use of TCM dialectical thinking to apply traditional Chinese medicine therapy to the treatment of clinical chemotherapy resistance and adverse reactions has achieved relatively satisfactory results. Based on the multi-molecule and multi-target effects of traditional Chinese medicine, it is of great practical significance to develop traditional Chinese medicine and its preparations that can reduce the adverse reactions of chemotherapy and improve the effect of chemotherapy, and to combine them with chemotherapy drugs.

[0003] The Suwen·Yin Yang Ying Xiang Da Lun Pian puts forward the theory of "Yang turns into Qi, Yin turns into shape". The formation of tumors is the accumulation of tangible things, that is, "Yin turns into shape". The essence is that Yin Qi cannot be transformed, and the reason is the dysfunction of "Yang turns into Qi". The Lingshu·The Beginning of All Diseases says: "The accumulation begins when it is cold." Many doctors believe that Yang deficiency, cold stagnation, and Yin Qi cannot be transformed are the root causes of tumor formation, and often dominate the process of its occurrence, development, and metastasis. Traditional Chinese medicine believes that Yang Qi is the root of promoting the operation of Qi in the whole body, involving metabolism and cell proliferation and differentiation. Kidney Yang is the root of Yang Qi in the whole body, which promotes and warms the functions of various organs and tissues in the human body, involving the proliferation and differentiation of immune and hematopoietic cells. The main mechanism of action of chemotherapy drugs is to kill cancer cells by interfering with the growth and division of cancer cells, such as blocking DNA replication and cell division. Traditional Chinese medicine believes that chemotherapy drugs are harsh and harmful to the human body's yang energy. On the one hand, they cause bone marrow suppression, which leads to inhibition of immune and hematopoietic cell differentiation and proliferation, and then leads to low immunity, anemia, etc. On the other hand, they cause poor functioning of the body's qi, blood, body fluids, viscera and meridians, aggravate the formation of a damp-heat and blood stasis environment, and promote the generation of chemotherapy resistance. Therefore, clinical treatment starts with qi and blood, strengthens the spleen and stomach, replenishes the kidney and replenishes essence, replenishes qi and blood, and promotes the purpose of strengthening the body and improving bone marrow hematopoietic function by promoting blood circulation and removing blood stasis. The present invention aims to provide a Chinese medicine preparation that can effectively and safely treat patients with side effects such as bone marrow suppression caused by chemotherapy in clinical practice. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides a traditional Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The invention provides a traditional Chinese medicine preparation for preventing and treating post-chemotherapy bone marrow suppression. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 1-15 parts of antler glue, 1-10 parts of astragalus, 1-10 parts of cassia twig, 1-10 parts of notoginseng, 1-10 parts of immature bitter orange, 1-10 parts of anemarrhena, and 1-10 parts of phellodendron.

[0009] Furthermore, the Chinese medicine preparation is made of the following raw materials in parts by weight: 1-10 parts of antler glue, 1-5 parts of astragalus, 1-5 parts of cinnamon twig, 1-5 parts of Panax notoginseng, 1-5 parts of Citrus aurantium, 1-5 parts of Anemarrhena asphodeloides, and 1-5 parts of Phellodendron chinense.

[0010] Furthermore, the Chinese medicine preparation is made of the following raw materials in parts by weight: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of Citrus aurantium, 3 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

[0011] The Chinese medicine preparation provided by the present invention is used to prepare a drug for preventing and treating bone marrow suppression after chemotherapy. The Chinese medicine preparation is prepared into granules, powders, oral liquids, pills, decoctions, lyophilized powders or other pharmaceutically acceptable oral dosage forms according to conventional pharmaceutical preparation methods in pharmacy and with medically acceptable adjuvants.

[0012] (III) Beneficial effects

[0013] The present invention takes TCM syndrome differentiation and treatment as the principle, rationally combines various medicines, and obtains a Chinese medicine preparation for preventing and treating post-chemotherapy bone marrow suppression made of antler glue, astragalus, cinnamon twig, Panax notoginseng, immature bitter orange, rhizoma anemarrhenae, and phellodendron. In the prescription, antler glue warms and nourishes the liver and kidney, nourishes essence and blood; "Qi is the leader of blood, and blood is the mother of Qi", astragalus is an important Qi-tonifying medicine, complements with antler glue, and jointly replenishes Qi and blood, which helps to promote blood circulation; cinnamon twig warms yang and transforms Qi, harmonizes Qi and blood, and plays a warming and tonic effect with antler glue and astragalus; Panax notoginseng promotes blood circulation and removes blood stasis, relieves swelling and relieves pain, helps to improve local blood circulation, and relieves pain; bitter orange eliminates Qi stagnation, resolves phlegm and disperses knots, helps to regulate Qi mechanism, and improves the microenvironment in the body; rhizoma anemarrhenae clears heat and nourishes yin, helps to balance yin and yang in the body, and prevents warming and tonic drugs from overheating and damaging yin; the heat-clearing and damp-drying effect of phellodendron complements with rhizoma anemarrhenae, and jointly regulates the damp-heat state in the body, and phellodendron also helps to remove bone steaming, which has a certain effect on improving the symptoms caused by yin deficiency and hyperactivity of fire. This prescription uses antler glue and astragalus as the main drugs, which work together to nourish blood and essence, nourish qi and raise yang; cinnamon twig as the assistant drug, warms and unblocks the meridians, and helps yang and transforms qi; Panax notoginseng and Citrus aurantium as the adjuvant drugs, respectively play the role of promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, and promoting qi and removing stagnation, and resolving phlegm and dispersing nodules; Anemarrhena asphodeloides and Phellodendron chinense as the guiding drugs, clearing away heat and nourishing yin, regulating the state of dampness and heat in the body, and preventing the warming and tonic drugs from overheating and damaging yin. The combination of these drugs can replenish qi and blood, promote blood circulation and remove blood stasis, reduce swelling and relieve pain, which is beneficial for preventing and treating bone marrow suppression after chemotherapy.

[0014] The Chinese medicine preparation of the present invention can reverse the bone marrow suppression caused by 5-FU chemotherapy by improving the peripheral blood picture and bone marrow pathological damage of chemotherapy mice. In addition, the Chinese medicine preparation of the present invention can improve the 5-FU chemotherapy effect by increasing the 5-FU tumor inhibition rate, inhibiting tumor weight, tumor volume and promoting tumor tissue necrosis. The Chinese medicine preparation of the present invention and the chemotherapy drug 5-FU have a certain effect of increasing efficacy and reducing toxicity, and are expected to be developed as a drug for preventing and treating bone marrow suppression after chemotherapy. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The tumor volume, tumor weight and tumor inhibition rate of mice in each group (A); Note: K: blank group, M: model group, Y: 5-FU group, G: high-dose group of the Chinese medicine preparation of the present invention, Z: medium-dose group of the Chinese medicine preparation of the present invention, D: low-dose group of the Chinese medicine preparation of the present invention; Compared with the model group, ## P<0.01, # P<0.05; compared with 5-FU group, **P<0.01, *P<0.05, the same below; HE staining results of tumor tissues of mice in each group (B).

[0016] Figure 2 The numbers of blood cells and bone marrow nucleated cells in each group of mice (A), bone marrow pathological Gissa staining results (B), and bone marrow HE staining results (C).

[0017] Figure 3 These are the levels of TGF-β1, G-CSF, TPO and EPO in the serum of mice in each group. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Example 1

[0020] A traditional Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy is prepared from the following raw materials in parts by weight: 4 parts of antler glue, 2 parts of astragalus, 1 part of cinnamon twig, 2 parts of notoginseng, 1 part of bitter orange, 1 part of anemarrhena, and 1 part of phellodendron.

[0021] The Chinese medicine preparation is prepared into granules, powders, oral liquids, pills, decoctions, lyophilized powders or other pharmaceutically acceptable oral dosage forms by conventional pharmaceutical preparation methods and with medically acceptable adjuvants.

[0022] Example 2

[0023] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 14 parts of antler glue, 8 parts of astragalus, 7 parts of cinnamon twig, 8 parts of Panax notoginseng, 6 parts of Citrus aurantium, 7 parts of Anemarrhena asphodeloides, and 7 parts of Phellodendron chinense.

[0024] Example 3

[0025] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 6 parts of antler glue, 4 parts of astragalus, 2 parts of cinnamon twig, 2 parts of Panax notoginseng, 3 parts of Citrus aurantium, 4 parts of Anemarrhena asphodeloides, and 2 parts of Phellodendron chinense.

[0026] Example 4

[0027] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 12 parts of antler glue, 6 parts of astragalus, 4 parts of cinnamon twig, 6 parts of Panax notoginseng, 8 parts of Citrus aurantium, 6 parts of Anemarrhena asphodeloides, and 7 parts of Phellodendron chinense.

[0028] Example 5

[0029] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 5 parts of antler glue, 2 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 1 part of Citrus aurantium, 2 parts of Anemarrhena asphodeloides, and 2 parts of Phellodendron chinense.

[0030] Example 6

[0031] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 8 parts of antler glue, 3 parts of astragalus, 4 parts of cinnamon twig, 2 parts of Panax notoginseng, 2 parts of Citrus aurantium, 2 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

[0032] Example 7

[0033] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 10 parts of antler glue, 4 parts of astragalus, 3 parts of cinnamon twig, 4 parts of Panax notoginseng, 3 parts of Citrus aurantium, 2 parts of Anemarrhena asphodeloides, and 2 parts of Phellodendron chinense.

[0034] Example 8

[0035] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is made of the following raw materials in parts by weight: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of Citrus aurantium, 3 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

[0036] Test example

[0037] The Chinese medicine preparation of the present invention improves the chemotherapy-induced bone marrow suppression in mice with H22 subcutaneous liver cancer tumor model

[0038] 1 Materials

[0039] 1.1 Experimental animals

[0040] KM female mice, body weight 18-22g, rearing conditions: indoor temperature 22-26℃, relative humidity 50%-70%, circadian rhythm 12h.

[0041] 1.2 Trial Drugs

[0042] The Chinese medicine preparation of the present invention comprises 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of immature bitter orange, 3 parts of Anemarrhena asphodeloides and 3 parts of Phellodendron chinense. The raw materials are weighed according to the above weight parts, washed, decocted for 40 minutes with 3 times of water, and the decocting is repeated for more than 2 times, the decoction is collected, and the decoction is concentrated to 1g / mL (1mL contains 1g of crude drug), and stored at 4°C for standby use.

[0043] 5-Fluorouracil (0.2 g, purchased from TargetMol).

[0044] 2 Methods

[0045] 2.1 Culture of H22 hepatocellular carcinoma cells

[0046] The frozen H22 liver cancer cells were placed in a 37°C water bath and thawed quickly within 1 min. After thawing, they were pipetted and transferred to a 2 mL centrifuge tube, centrifuged, the supernatant was discarded, and 1.5 mL of normal saline was added to dilute the H22 cells to 1×10 7 / mL cell suspension, extract the cell suspension with a 2.5mL sterile syringe, and inject it into mice by intraperitoneal injection for subculture, a total of 3 mice, 0.5mL each. After 7 days of feeding, the abdominal cavity of the mouse is observed to be swollen, and the ascites can be collected at this time. The mouse is killed by cervical vertebrae, ascites is extracted, and the tumor cells are diluted and suspended with saline to adjust the cell concentration. The whole process requires aseptic operation.

[0047] 2.2 Model establishment

[0048] Sixty KM mice were randomly divided into 10 mice as the blank group, and the remaining 50 mice were subcutaneously inoculated with H22 mouse liver cancer cell suspension in the axilla of the right forelimb, 0.2 mL / mouse, to establish the H22 mouse solid tumor model. The blank group was injected with the same volume of normal saline.

[0049] 2.3 Grouping and Dosing

[0050] Tumor formation was observed 5 days after inoculation. When the tumor grew to 100 mm 3At the same time, the mice were divided into 6 groups according to the random number table method, including the model group, 5-FU group, high, medium and low dose groups of the Chinese medicine preparation of the present invention, with 10 mice in each group. The mice without modeling were given an equal volume of pure water gavage once a day as the blank control group. The 5-FU group and the high, medium and low dose groups of the Chinese medicine preparation of the present invention were all given 5-FU intraperitoneal injection of 30 mg / (kg·d). At the same time, the high, medium and low dose groups of the Chinese medicine preparation of the present invention were given 12 g / kg, 6 g / kg and 3 g / kg (calculated by crude drug amount) of the Chinese medicine preparation of the present invention, respectively, once a day for 14 consecutive days; the model group was given an equal volume of normal saline intraperitoneal injection, once every other day for 14 consecutive days.

[0051] 2.4 Index detection

[0052] 2.4.1 Tumor volume, tumor weight and tumor inhibition rate of mice

[0053] The tumor mass was quickly removed under sterile conditions, and the morphology of the mouse tumor mass was observed with the naked eye and photographed for record. The tumor mass was weighed with an analytical balance and the tumor inhibition rate was calculated; the vertical diameter and the longest diameter of the tumor were measured with a vernier caliper, and the tumor volume was calculated according to the following formula:

[0054] Tumor volume = 0.5236 × ab 2 (In the formula, a is the longest diameter of the tumor and b is the vertical diameter of the tumor).

[0055] 2.4.2 Detection of the number of white blood cells, red blood cells and platelets in mouse peripheral blood

[0056] The blood of mice was collected by the eyeball blood sampling method. About 0.5 mL of blood was collected from each mouse and put into an anticoagulant centrifuge tube. A diluent was added. 20 μL of the mouse blood in the anticoagulant centrifuge tube was drawn with a pipette and added into a 2 mL centrifuge tube. The number of peripheral white blood cells, red blood cells, and platelets of the mice was detected using a blood cell analyzer.

[0057] 2.4.3 Detection of G-CSF, TPO, EPO and TGF-β1 in mouse serum

[0058] The blood of mice was collected by eyeball blood sampling method and centrifuged at 3000r / min for 10min. The serum was separated and stored at -20℃ for later use. The levels of G-CSF, TPO, EPO and TGF-β1 in mouse serum were detected by enzyme-linked immunosorbent assay (ELISA).

[0059] 2.4.4 Detection of mouse bone marrow nucleated cells by Wright-Giemsa staining

[0060] The left femur was separated under sterile conditions, and both ends of the femur were cut off. The bone marrow cavity was flushed with physiological saline using a No. 6 needle. The flushed bone marrow cells were made into a single bone marrow cell suspension, and 5 μL was aspirated to prepare a cell smear. After natural drying, 2 to 3 drops of Wright-Giemsa composite stain were added and stained for 1 to 2 minutes. PBS buffer was added, and the smear was gently shaken to mix it evenly with the Wright-Giemsa stain, and then stained for another 3 to 5 minutes. The smear was washed with water and placed under a high-power microscope to count the cells.

[0061] 2.4.5 Observation of tumor mass and bone marrow pathological changes in mice by HE staining

[0062] After blood collection, the mice were killed by sodium pentobarbital anesthesia, the axillary tumors were removed, the right femurs of the mice were peeled off (decalcified with decalcification solution), fixed with 10% formaldehyde solution, dehydrated, embedded, transparent, sliced, dewaxed, and stained with hematoxylin-eosin. The pathological sections were observed using a digital pathological section scanner, and the tumor tissue and bone marrow pathology of the chemotherapy mice were graded and evaluated according to the literature methods.

[0063] Table 1 Evaluation of bone marrow pathological grading in chemotherapy mice (n=10)

[0064] level Damage level - The bone marrow tissue structure is complete, the hematopoietic cells are abundant, full of nucleated cells and evenly distributed + A small number of vacuolar areas, more fat cells, less nucleated cells ++ The vacuolar area increases, fat cells are more common, and the nucleated cells decrease and are more sparsely distributed. +++ The vacuolar area increased significantly, the fat cells increased significantly, and the nucleated cells decreased and became disordered. ++++ There are a large number of vacuolar areas, a significant increase in fat cells, sparse and disordered distribution of nucleated cells, and a significant decrease in density

[0065] Table 2 Evaluation of tumor tissue pathological grade in chemotherapy mice (n=10)

[0066]

[0067] 3 Statistical analysis

[0068] SPSS17.0 was used for data processing and the statistical results were presented as mean ± standard deviation. One-way ANOVA was used for comparison among the groups. The LSD method was used for equal variance test and the Games-Howell method was used for unequal variance test. The Ridit test was used for ranked data.

[0069] 4. Results

[0070] 4.1 The Chinese medicine preparation of the present invention can improve the tumor volume, tumor weight and pathological changes of mice and increase the tumor inhibition rate

[0071] Compared with the model group, the tumor weight and tumor volume in the 5-FU group were significantly reduced (P < 0.01). Compared with the 5-FU group, the high and medium doses of the Chinese medicine preparation of the present invention could significantly reduce the tumor weight (P < 0.01) and tumor volume (P < 0.01, P < 0.05). The low dose of the Chinese medicine preparation of the present invention could significantly reduce the tumor weight (P < 0.05) and tumor volume (P < 0.05). Each dose group of the Chinese medicine preparation of the present invention could significantly increase the tumor inhibition rate. Figure 1 -A.

[0072] HE staining results showed that the tumor tissue cells in the model group were closely arranged and evenly distributed, the cell nuclei were deeply stained, the nuclear-cytoplasmic ratio was high, nuclear division images were visible, a large number of granulocytes infiltrated in the tumor stroma, and no obvious cell necrosis was observed. Tumor tissue tumor cells in each dose group of the Chinese medicine preparation of the present invention were loosely arranged, vacuolated, and unevenly distributed to varying degrees. Tumor cell focal necrosis was observed in many places, and the necrotic cell nuclei were condensed and fragmented. The number of mice with tumor tissue necrosis in each dose group of the Chinese medicine preparation of the present invention increased significantly. The results are shown in Tables 3 and 4. Figure 1 -B.

[0073] Table 3 Results of evaluation of tumor tissue pathology in chemotherapy mice (n=10)

[0074]

[0075]

[0076] Note: Compared with 5-FU group, *P<0.05, **P<0.01, compared with model group, # P<0.05, ## P<0.01

[0077] 4.2 The Chinese medicine preparation of the present invention can increase the number of blood cells and bone marrow nucleated cells in mice and improve bone marrow pathological changes

[0078] The results of peripheral blood cell detection showed that compared with the model group, the 5-FU group could significantly reduce the number of PLT, RBC, and WBC in the peripheral blood of mice (P < 0.01); compared with the 5-FU group, the high and medium doses of the Chinese medicine preparation of the present invention could significantly increase the number of PLT, RBC, and WBC (P < 0.01), and the low dose group of the Chinese medicine preparation of the present invention could also significantly increase the number of RBC, PLT, and WBC (P < 0.05). Figure 2 -A.

[0079] Gissam staining results showed that compared with the model group, the 5-FU group could significantly reduce the number of nucleated cells in the bone marrow of mice (P < 0.01); compared with the 5-FU group, each dose group of the Chinese medicine preparation of the present invention could significantly increase the number of nucleated cells in the bone marrow of chemotherapy mice (P < 0.01). Figure 2 -B.

[0080] The results of bone marrow HE staining showed that the tissue structure of the femoral bone marrow of the mice in the blank group and the model group was intact, with abundant hematopoietic cells and evenly distributed nucleated cells; the bone marrow tissue structure of the mice in the 5-FU group was abnormal, the number of hematopoietic cells in the bone marrow cavity was significantly reduced, a large number of vacuolar areas were visible, the number of fat cells increased, and the distribution of nucleated cells was sparse, disordered, and the density decreased; compared with the mice in the 5-FU group, the number of nucleated cells in the bone marrow of the mice in each dose group of the Chinese medicine preparation of the present invention increased significantly, the number of fat cells decreased, the bone marrow tissue damage was milder, and the number of mice with improved bone marrow damage increased significantly, the results are shown in Table 4 and Figure 2 -C.

[0081] Table 4 Results of evaluation of bone marrow pathological grading in chemotherapy mice (n=10)

[0082]

[0083] Note: Compared with 5-FU group, *P<0.05, **P<0.01, compared with model group, # P<0.05, ## P<0.01

[0084] 4.3 The Chinese medicine preparation of the present invention can improve the levels of TGF-β1, G-CSF, TPO and EPO in mouse serum

[0085] The results of ELISA test showed that compared with the model group, the 5-FU group could significantly increase the TGF-β1 content in the serum of chemotherapy mice (P < 0.05), and significantly reduce the G-CSF, TPO, and EPO contents in the serum (P < 0.01); compared with the 5-FU group, the high- and medium-dose groups of the Chinese medicine preparation of the present invention could significantly reduce the TGF-β1 content in the serum of mice (P < 0.01), and increase the G-CSF, TPO, and EPO contents in the serum (P < 0.01); the low-dose group of the Chinese medicine preparation of the present invention could significantly reduce the TGF-β1 in the serum of mice, and increase the G-CSF, TPO, and EPO contents (P < 0.05, P < 0.01); the specific results are shown in Figure 3 .

[0086] 5 Conclusion

[0087] The above experimental results show that the Chinese medicine preparation of the present invention can reverse the bone marrow suppression caused by 5-FU chemotherapy by improving the peripheral blood picture and bone marrow pathological damage of chemotherapy mice. In addition, the Chinese medicine preparation of the present invention can improve the 5-FU chemotherapy effect by increasing the 5-FU tumor inhibition rate, inhibiting tumor weight, tumor volume and promoting tumor tissue necrosis. The Chinese medicine preparation of the present invention combined with the chemotherapy drug 5-FU has a certain effect of increasing efficacy and reducing toxicity, and has potential development and utilization value.

[0088] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy, characterized in that: The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 1-15 parts of antler glue, 1-10 parts of astragalus, 1-10 parts of cassia twig, 1-10 parts of notoginseng, 1-10 parts of immature bitter orange, 1-10 parts of anemarrhena, and 1-10 parts of phellodendron.

2. A Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy according to claim 1, characterized in that: The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 1-10 parts of antler glue, 1-5 parts of astragalus, 1-5 parts of cassia twig, 1-5 parts of notoginseng, 1-5 parts of immature bitter orange, 1-5 parts of anemarrhena, and 1-5 parts of phellodendron.

3. A Chinese medicine preparation for preventing and treating bone marrow suppression after chemotherapy according to claim 1, characterized in that: The Chinese medicine preparation is prepared from the following raw materials in parts by weight: 6 parts of antler glue, 3 parts of astragalus, 3 parts of cinnamon twig, 3 parts of Panax notoginseng, 3 parts of Citrus aurantium, 3 parts of Anemarrhena asphodeloides, and 3 parts of Phellodendron chinense.

4. Use of the Chinese medicine preparation according to any one of claims 1 to 3 for preparing a drug for preventing and treating bone marrow suppression after chemotherapy, characterized in that: The Chinese medicine preparation is prepared into granules, powders, oral liquids, pills, decoctions, lyophilized powders or other pharmaceutically acceptable oral dosage forms by conventional pharmaceutical preparation methods and with medically acceptable adjuvants.

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