Traditional Chinese medicine composition for preventing or improving alcoholic liver and kidney injury and application thereof

Abstract

The invention relates to a traditional Chinese medicine composition for preventing or improving alcoholic liver and kidney injury and pharmaceutical application thereof, and the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-50 parts of kudzuvine root, 5-50 parts of kudzuvine flower, 6-45 parts of hovenia acerba, 10-60 parts of sea buckthorn fruit, 10-60 parts of hawthorn, 3-20 parts of ginger bamboo shavings, 6-40 parts of schisandra chinensis, 10-50 parts of rhodiola rosea and 6-50 parts of liquorice. The traditional Chinese medicine composition can effectively prevent or treat alcoholic liver injury and alcoholic kidney injury and reduce gastrointestinal irritation.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and specifically relates to a traditional Chinese medicine composition for preventing or improving alcoholic liver and kidney damage and a use thereof. Background Art

[0003] Ethanol is the main component of alcoholic beverages, and the metabolism of ethanol is essentially an enzymatic decomposition process. After alcohol enters the human body, it is mainly absorbed by the digestive system, including the stomach and intestines. About 70% of the ethanol is absorbed by the upper small intestine, and about 30% is absorbed by the stomach. It is then circulated in the blood to the liver for metabolism. About 90% of the ethanol is metabolized in the liver, and the remaining 10% of the ethanol is excreted from the body in the form of breath, sweat, and kidney metabolism in the form of urine. Alcohol and its metabolites cause lipid metabolism disorders, endoplasmic reticulum stress, oxidative stress, inflammation, and cell apoptosis, which trigger alcoholic liver damage, and then gradually progress to alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis, alcoholic cirrhosis, and even liver cancer.

[0004] Since there are a large number of drugs with liver protection, anti-inflammatory, antioxidant and energy metabolism regulation in the treasure house of traditional Chinese medicine, and there are many drugs with medicinal and edible homology, which have considerable edibility and safety, the current traditional Chinese medicine for the treatment of alcoholic liver injury mainly focuses on blood circulation and blood stasis drugs, diuretic and dampness-inducing drugs, antipyretic and heat-clearing drugs, qi-regulating and qi-tonifying drugs, and alcohol-resolving drugs, and most prescriptions are mainly alcohol-resolving drugs, or focus on liver protection. For example, CN117899145A discloses a Chinese medicine composition and medicine for preventing and treating alcoholic liver injury, which is made of the following raw materials: 20-40 parts of Pueraria root, 10-25 parts of Hovenia dulcis, 3-15 parts of Bupleurum, and 5-20 parts of Paeonia lactiflora; the four drugs are used together to play the effects of alcohol-resolving and restlessness, clearing heat and dampness, soothing the liver and promoting blood circulation, and have the effects of alcohol-resolving and liver-protecting, and are used for acute alcohol poisoning and chronic alcoholic liver injury. CN118105432A discloses a Chinese medicine composition for resisting acute alcoholic liver injury and its use. The Chinese medicine composition is prepared from the following raw materials in parts by weight: 25-60 parts of Gynostemma pentaphyllum, 3-12 parts of cinnamon bark, and 30-80 parts of rattan tea. The Chinese medicine composition can effectively reduce the increase of liver function transaminases in C57BL / 6 mice induced by acute alcohol feeding; reduce pathological changes such as lipid accumulation and inflammatory infiltration in liver tissue, improve liver injury, and prevent and treat acute alcoholic liver injury by inhibiting oxidative stress, endoplasmic reticulum stress, cell apoptosis and inflammatory signaling pathways caused by alcohol. CN117044943A discloses a composition for protecting alcoholic liver damage, its preparation method and application, and its preparation raw materials include the following components in parts by weight: 1-6 parts of peony seed oil, 1-8 parts of kudzu root, 1-4 parts of Hovenia dulcis, and 1-2 parts of Gardenia jasminoides; the above four components cooperate with each other and work together to inhibit oxidative stress and lipid peroxidation damage as the main function, while taking into account anti-inflammatory, effectively inhibiting the secondary damage of the liver caused by the inflammatory reaction induced by endogenous toxins, treating both the symptoms and the root cause, and significantly improving the symptoms of alcoholic liver damage. KR102043813B1 discloses a composition for protecting the liver from alcoholic liver damage, which is made of kudzu root extract, tangerine peel extract, red dog extract, licorice extract, wolfberry extract, and ginseng extract. However, Hovenia dulcis is sweet and sour in nature, and is neutral. It can detoxify alcohol, quench thirst and relieve restlessness, stop vomiting, and promote urination. However, for those with weak spleen and stomach, Hovenia dulcis can irritate the gastrointestinal mucosa, thereby causing gastrointestinal dysfunction and aggravating symptoms, so it is not recommended to take it for a long time.

[0005] In addition, the current organ damage of alcohol is mainly focused on liver damage, but some clinical studies have shown that drinking has a great impact on the kidneys, mainly manifested as decreased glomerular filtration rate, increased serum creatinine and urea nitrogen, and proteinuria, leading to alcoholic kidney damage. Ethanol intake may increase the expression of other potential free radicals in the kidneys, which is related to the enzyme family of nitric oxide synthase. Nitric oxide synthase promotes the production of nitric oxide. If it is produced in excess, it can react with other molecules and produce free radicals, thereby triggering tissue damage in the kidneys. However, there is currently insufficient research on traditional Chinese medicine and traditional Chinese medicine compound prescriptions for alcoholic kidney damage, and there is a lack of related traditional Chinese medicine products. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Chinese medicine composition for preventing or improving alcoholic liver and kidney damage and its pharmaceutical use, which can effectively prevent or treat alcoholic liver damage and alcoholic kidney damage, and reduce gastrointestinal irritation, especially when alcohol has certain damage to the gastrointestinal tract, and is also suitable for people with spleen and stomach deficiency and cold.

[0007] The technical solution of the present invention is summarized as follows:

[0008] A traditional Chinese medicine composition for preventing or improving alcoholic liver and kidney damage is prepared from the following traditional Chinese medicine raw materials in the following weight proportions: 5-50 parts of kudzu root, 5-50 parts of kudzu flower, 6-45 parts of hovenia dulcis, 10-60 parts of seabuckthorn fruit, 10-60 parts of hawthorn, 3-20 parts of ginger bamboo shavings, 6-40 parts of schisandra chinensis, 10-50 parts of rhodiola rosea, and 6-50 parts of liquorice.

[0009] It is preferably made of the following Chinese medicinal raw materials in the following weight proportions: 5-40 parts of Pueraria root, 5-30 parts of Pueraria flower, 6-35 parts of Hovenia dulcis, 10-45 parts of Seabuckthorn fruit, 10-40 parts of Hawthorn, 3-15 parts of Anemarrhena asphodeloides, 6-35 parts of Schisandra chinensis, 10-35 parts of Rhodiola rosea, and 6-30 parts of Licorice.

[0010] It is preferably made of the following Chinese medicinal raw materials in the following weight proportions: 10-30 parts of Pueraria root, 10-30 parts of Pueraria flower, 10-25 parts of Hovenia dulcis, 20-40 parts of Seabuckthorn fruit, 15-30 parts of Hawthorn, 3-10 parts of Anemarrhena asphodeloides, 9-20 parts of Schisandra chinensis, 10-25 parts of Rhodiola rosea, and 6-20 parts of Licorice.

[0011] More preferably, it is made of the following Chinese medicinal raw materials in the following weight proportions: 25 parts of Pueraria root, 15 parts of Pueraria flower, 15 parts of Hovenia dulcis, 30 parts of Seabuckthorn fruit, 25 parts of Crataegus pinnatifida, 6 parts of Anemarrhena asphodeloides, 15 parts of Schisandra chinensis, 10 parts of Rhodiola rosea, and 10 parts of Licorice.

[0012] Among them, Pueraria root is the dried root of Pueraria lobata or Pueraria lobata of the Leguminosae family, containing isoflavone components puerarin, puerarin xyloside, soybean flavonoids, soybean flavonoids, β-sitosterol, and arachidic acid, and contains a large amount of starch (the content in fresh Pueraria root is 19-20%). It has the effects of raising yang and relieving muscles, clearing rashes and stopping diarrhea, and eliminating restlessness and stopping fever. "Compendium of Materia Medica" records: It is cool in nature and is easy to cause vomiting. People with cold stomach should use it with caution.

[0013] Kudzu flower is the flower of the leguminous plant kudzu or sweet kudzu vine. It has the effect of relieving alcohol and quenching thirst. It is used to treat thirst caused by drunkenness, fever, thirst caused by alcohol injuries, loss of appetite, vomiting, acid reflux, vomiting blood, and intestinal bleeding.

[0014] Hovenia dulcis is the fruit with fleshy stalks of the plant Hovenia dulcis of the Rhamnaceae family. It tastes sweet and sour, is neutral in nature, non-toxic, and can treat drunkenness, irritability, thirst, vomiting, and constipation. Depei Materia Medica records that it is forbidden for people with spleen and stomach deficiency and coldness.

[0015] Seabuckthorn is the dried mature fruit of Hippophae rhamnoides L., a plant of the genus Hippophae in the family Elaeagnaceae. The fruit is harvested in autumn and winter when it is ripe or frozen, and then removed of impurities and dried or steamed and dried. Nature and flavor: sour, astringent, warm. It has the functions of relieving cough and expectoration, digesting food and removing stagnation, and promoting blood circulation and dispersing blood stasis. It is used for cough with sputum, indigestion, abdominal pain due to food accumulation, amenorrhea due to blood stasis, and swelling due to falls.

[0016] Hawthorn is the dried mature fruit of the plant Shanlihong or Shan of the Rosaceae family. It helps digestion and invigorates the stomach, and promotes qi and dissipates blood stasis. It is used for meat stagnation, stomach distension, diarrhea and abdominal pain, blood stasis and amenorrhea, postpartum blood stasis, heart and abdomen stabbing pain, hernia pain, and hyperlipidemia. The effect of charred hawthorn on digestion and dispersing stagnation is enhanced. It is used for meat stagnation and diarrhea.

[0017] Bamboo Ru is the dried middle layer of the stem of the grass family plants green bamboo, big head bamboo or light bamboo. It clears away heat and resolves phlegm, relieves restlessness and stops vomiting. It is used for cough due to phlegm heat, phlegm with gallbladder fire, restlessness and vomiting, palpitations and loss of eyesight, stroke and phlegm, tongue stiffness and inability to speak, stomach heat and vomiting, nausea during pregnancy, and restless fetuses.

[0018] Schisandra chinensis is the dried mature fruit of the Magnoliaceae plant Schisandra chinensis or Schisandra sphenanthera. It is sour, sweet and warm in nature. It has the functions of astringing and strengthening, replenishing qi and promoting body fluid, tonifying the kidney and calming the mind. It is used for chronic cough, asthenia, nocturnal emission, spermatorrhea, enuresis, frequent urination, chronic diarrhea, spontaneous sweating, night sweats, thirst due to loss of body fluid, shortness of breath and weak pulse, internal heat and thirst, palpitations and insomnia.

[0019] Rhodiola rosea is a plant of the genus Rhodiola in the family Crassulaceae. The whole plant of Rhodiola rosea is used as medicine. It is sweet, astringent and cold in nature and flavor. Functions and indications: clearing the lungs and relieving cough, stopping bleeding and stopping leucorrhea. It is used for cough due to lung heat, hemoptysis and leucorrhea; it can be used externally to treat injuries caused by falls and burns.

[0020] The hawthorn is stir-fried hawthorn, and the licorice is roasted licorice.

[0021] The preparation method of the Chinese medicine composition of the present invention comprises the following steps: mixing various Chinese medicine raw materials, and then adding water or alcohol aqueous solution for extraction.

[0022] Preferably, the alcohol aqueous solution is selected from 50-90% methanol, ethanol, propanol or butanol solution, more preferably 50%, 60%, 70%, 75%, 80%, 85% ethanol solution.

[0023] The present invention also provides a traditional Chinese medicine preparation, which comprises any one of the traditional Chinese medicine compositions described above, and auxiliary materials.

[0024] The above-mentioned traditional Chinese medicine preparation is selected from one of decoction, powder, granule, tablet, oral liquid, lotion and emulsion.

[0025] The present invention also provides the use of the above-mentioned Chinese medicine composition or Chinese medicine preparation in preparing a medicine for improving alcoholic liver and kidney damage.

[0026] The present invention also provides the use of the above-mentioned Chinese medicine composition or Chinese medicine preparation in the preparation of a drug for improving alcoholic liver and kidney damage, wherein the population is a population with both spleen and stomach deficiency and coldness or spleen and stomach susceptible to irritation.

[0027] Beneficial effects: It can effectively prevent or treat alcoholic liver damage and alcoholic kidney damage, and reduce gastrointestinal irritation, especially when alcohol has caused certain damage to the gastrointestinal tract. It is also suitable for people with spleen and stomach deficiency. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with specific embodiments.

[0029] Example 1

[0030] Take 25g of Pueraria root, 15g of Pueraria flower, 15g of Hovenia dulcis, 30g of sea buckthorn fruit, 25g of stir-fried hawthorn, 6g of Rhizoma Zingiberis, 15g of Schisandra chinensis, 10g of Rhodiola rosea, and 10g of Radix Glycyrrhizae. Soak the Pueraria root, Hovenia dulcis, sea buckthorn fruit, stir-fried hawthorn, Rhizoma Zingiberis, Schisandra chinensis, Rhodiola rosea and Rhizoma Glycyrrhizae in 15 times the amount of water for 30 minutes, then add Pueraria flower and heat to reflux and extract for 2 hours, filter, collect the filtrate, continue to add 8 times the amount of water to the residue, heat to reflux and extract for 1 hour, filter, combine the two filtrates, and concentrate the liquid to 3g / ml to obtain the product.

[0031] Example 2

[0032] Take 20g of Pueraria root, 25g of Pueraria flower, 10g of Hovenia dulcis, 20g of Seabuckthorn fruit, 35g of stir-fried hawthorn, 9g of Rhizoma Zingiberis, 20g of Schisandra chinensis, 30g of Rhodiola rosea, and 30g of Radix Glycyrrhizae. Soak the Pueraria root, Hovenia dulcis, Seabuckthorn fruit, stir-fried hawthorn, Rhizoma Zingiberis, Schisandra chinensis, Rhodiola rosea and Rhizoma Glycyrrhizae in 12 times the amount of water for 30 minutes, then add Pueraria flower and heat to reflux and extract for 1.5 hours, filter, collect the filtrate, continue to add 10 times the amount of water to the residue, heat to reflux and extract for 1.5 hours, filter, combine the two filtrates, concentrate, and add sweeteners and preservatives to make an oral solution.

[0033] Example 3

[0034] Take 15g of Pueraria root, 40g of Pueraria flower, 30g of Hovenia dulcis, 12g of Seabuckthorn fruit, 10g of stir-fried Hawthorn, 12g of Rhizoma Zingiberis, 6g of Schisandra chinensis, 40g of Rhodiola rosea and 35g of Radix Glycyrrhizae. Soak the Pueraria root, Hovenia dulcis, Seabuckthorn fruit, stir-fried Hawthorn, Rhizoma Zingiberis, Schisandra chinensis, Rhodiola rosea and Radix Glycyrrhizae in 15 times the amount of water for 30 minutes, then add the Pueraria flower, use steam distillation to collect the distillate, then add a preservative and sterilize to make a dew.

[0035] Example 4

[0036] Take 10g of Pueraria root, 15g of Pueraria flower, 9g of Hovenia dulcis, 10g of Seabuckthorn fruit, 35g of stir-fried hawthorn, 6g of Rhizoma Zingiberis, 25g of Schisandra chinensis, 10g of Rhodiola rosea, and 5g of Radix Glycyrrhizae. Add 10 times the amount of 65% ethanol to the Pueraria root, Hovenia dulcis, Seabuckthorn fruit, stir-fried hawthorn, Rhizoma Zingiberis, Schisandra chinensis, Rhodiola rosea, Rhizoma Zinnicarp and Pueraria flower, soak them for 30 minutes, heat and reflux for 1.5 hours, filter, collect the filtrate, and then add 10 times the amount of 70% ethanol to the residue for reflux extraction for 1.5 hours, filter, combine the filtrate, recover the ethanol under reduced pressure, concentrate to an extract, dry, crush and sieve, add dextrin and magnesium stearate to make granules.

[0037] Example 5

[0038] Take 30g of Pueraria root, 9g of Pueraria flower, 35g of Hovenia dulcis, 15g of Seabuckthorn fruit, 40g of stir-fried hawthorn, 6g of Rhizoma Zingiberis, 10g of Schisandra chinensis, 25g of Rhodiola rosea, and 9g of Radix Glycyrrhizae. Add 12 times the amount of 70% to the Pueraria root, Hovenia dulcis, Seabuckthorn fruit, stir-fried hawthorn, Rhizoma Zingiberis, Schisandra chinensis, Rhodiola rosea, Rhizoma Zingiberis and Radix Glycyrrhizae and Pueraria flower, soak them for 30 minutes, heat and reflux for extraction for 2 hours, filter, collect the filtrate, and then continue to add 10 times the amount of 85% ethanol to the residues for reflux extraction for 1 hour, filter, combine the filtrate, recover the ethanol under reduced pressure, concentrate to an extract, dry, crush and sieve, add starch, microcrystalline cellulose and sodium carboxymethyl cellulose to make granules, and then add magnesium stearate and silicon dioxide to press into tablets.

[0039] Comparative Example 1

[0040] Take 25g of Pueraria root, 15g of Pueraria flower, 15g of Hovenia dulcis, 30g of Hippophae rhamnoides fruit, 15g of Schisandra chinensis, 10g of Rhodiola rosea, and 10g of Radix Glycyrrhizae uralensis, and prepare them according to the same method as in Example 1.

[0041] Comparative Example 2

[0042] Take 25g of Pueraria root, 15g of Pueraria flower, 15g of Hovenia dulcis, 30g of Seabuckthorn fruit, 25g of stir-fried Hawthorn, 15g of Schisandra chinensis, and 10g of roasted Licorice root, and the preparation method is the same as that in Example 1.

[0043] Comparative Example 3

[0044] Take 25g of Pueraria root, 15g of Pueraria flower, 15g of Hovenia dulcis, 30g of Hippophae rhamnoides fruit, 25g of Red Peony Root, 6g of Rhizoma Anemarrhenae, 15g of Schisandrae Chinensis, 10g of Bupleurum Root, and 10g of Radix Glycyrrhizae, and prepare them according to the same method as in Example 1.

[0045] Comparative Example 4

[0046] Take 25g of Pueraria root, 15g of Pueraria flower, 15g of Hovenia dulcis, 30g of Lycium barbarum, 25g of stir-fried hawthorn, 6g of dried tangerine peel, 15g of Schisandra chinensis, 10g of Rhodiola rosea, and 10g of roasted Licorice root, and the preparation method is the same as that in Example 1.

[0047] Comparative Example 5

[0048] Take 25g of Pueraria root, 15g of Pueraria flower, 15g of Hovenia dulcis, 30g of Seabuckthorn fruit, 25g of stir-fried Hawthorn, 15g of Schisandra chinensis, 10g of Rhodiola rosea, and 10g of roasted Licorice root, and the preparation method is the same as Example 1.

[0049] Experimental Example 1

[0050] Alcoholic liver injury experiment

[0051] 70 SPF male Kunming mice with a mass of (20±2)g were randomly divided into 7 groups, namely, blank control group, model group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group, with 10 mice in each group. Among them, the blank control group and the model group were fed with normal diet and water for 14 days, and the Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group were gavaged with the same dose of the corresponding Chinese medicine extract, once a day, for 14 consecutive days. Starting from the 15th day, the Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group were gavaged with 52% liquor at a dose of 12mL / (kg·BW) 3 hours after the gavage every day, and the model group was also gavaged with the above liquor at the same dose for 3 consecutive days, and the blank control group still had normal diet and water.

[0052] After the experiment, the eyeballs were removed to collect blood, and the upper serum was collected after centrifugation and stored for later use. After the blood collection was completed, the mice were killed by cervical dislocation, and the liver tissue was collected and stored for later use. Then, the SOD, MDA, AST, ALT, and GSH indicators in the serum and the GSH, TG, and MDA levels in the liver tissue were measured respectively.

[0053] Table 1 Effects on serum SOD, MDA, AST, ALT and GSH in mice with alcoholic liver injury

[0054]

[0055] Table 2 Effects on GSH, TG and MDA levels in liver tissue of mice with alcoholic liver injury

[0056] Group GSH (ng / g) TG (nmol / g) MDA (nmol / g) Blank control group 0.31±0.062 0.021±0.0011 2.11±0.32 Model Group 0.15±0.083 0.032±0.0021 3.47±0.42 Example 1 Group 0.26±0.043 0.022±0.0016 2.46±0.27 Comparative Example 1 0.23±0.038 0.026±0.0018 2.81±0.49 Comparative Example 2 0.24±0.049 0.025±0.0031 2.74±0.51 Comparative Example 3 0.21±0.062 0.027±0.0028 2.63±0.45 Comparative Example 4 0.22±0.053 0.026±0.0013 2.67±0.44

[0057] The results in Table 1 and Table 2 show that the Chinese medicine composition of the present invention has a significant effect on acute alcoholic liver injury. Compared with the prescriptions of Comparative Examples 1-4, the Chinese medicine composition of the present invention also has obvious differences in alcoholic liver injury and has a better effect.

[0058] Experimental Example 2

[0059] Alcoholic renal injury experiment

[0060] 70 Wistar rats were randomly divided into 7 groups, 10 in each group, divided into blank control group, model group, embodiment 1 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, wherein blank control group was gavaged with normal saline (20ml / kg, 2 times / day), model group, embodiment 1 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group were gavaged with 52 ° Erguotou (20ml / kg, 2 times / day), for 6 consecutive weeks. After modeling, blank control group and model group were gavaged with normal saline (20ml / kg) every day, embodiment 1 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group were gavaged with corresponding Chinese medicine extract respectively, and the dosage used was identical, and each group was gavaged for 4 weeks. After 4 weeks, each group of rat blood was collected to detect blood creatinine and blood urea nitrogen.

[0061] Table 3 Effects on BUN\Scr in rats with alcoholic renal injury

[0062] Group BUN (mmol / L) Scr(umol / L) Blank control group 5.33±0.69 37.1±6.54 Model Group 19.8±1.96 96.4±7.72 Example 1 Group 7.14±1.48 48.7±8.43 Comparative Example 1 14.6±1.32 79.4±5.57 Comparative Example 2 15.1±2.41 82.9±6.82 Comparative Example 3 13.8±2.47 70.1±6.39 Comparative Example 4 17.3±3.85 78.5±7.53

[0063] The results in Table 3 show that the Chinese medicine composition of the present invention has significant differences in improving alcoholic renal injury compared with the Chinese medicine compositions of Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, etc., and can better prevent and treat alcoholic renal injury.

[0064] Experimental Example 3

[0065] Hovenia dulcis in the present invention is sweet, sour, and flat in nature. It is used with caution for people with spleen and stomach deficiency and coldness. In clinical applications, it is found that long-term consumption will damage the spleen and stomach yang qi and aggravate the symptoms of spleen and stomach deficiency and coldness. Therefore, people with spleen and stomach deficiency and coldness who have symptoms such as stomachache, abdominal pain, diarrhea, and loose stools may be stimulated by Hovenia dulcis to the gastrointestinal mucosa, thereby causing gastrointestinal dysfunction and aggravating symptoms, and are therefore not suitable for long-term use. Therefore, the present invention further studies the possible effects of the Chinese medicine composition of the present invention on the gastric mucosa.

[0066] Thirty rats were randomly divided into three groups, namely, a blank control group, Example 1 group, and Comparative Example 5 group, with 10 rats in each group. The Example 1 group and Comparative Example 5 group were gavaged with corresponding high doses of Chinese medicine extracts twice a day for 8 weeks, and the blank control group was gavaged with normal saline. After 8 weeks, the thickness of gastric mucus, gastric acid secretion, PGE 2 .

[0067] Gastric acid secretion detection method: After rats were anesthetized intraperitoneally with sodium phenobarbital, the abdominal cavity was opened at the midline of the abdomen, and an incision with a diameter of 0.3 cm was made in the duodenum 1 cm away from the pylorus. A catheter was inserted into the pylorus for drainage, and the pylorus was ligated; then an incision was made at the cardia, and a plastic catheter was inserted into the fundus for perfusion. The cardia incision was ligated, and the abdominal wall was sutured; 37°C saline was perfused into the stomach through the perfusion tube. The drainage fluid was collected, and then the drainage fluid was measured to calculate the gastric acid secretion.

[0068] Method for detecting gastric mucus thickness: After the above-mentioned gastric acid determination, the stomach of the rat is removed by laparotomy, and the stomach is cut open along the greater curvature of the stomach, the gastric cavity is everted to expose the gastric mucosa, and the ex vivo gastric mucosa is gently rinsed with saline; saline is injected into the submucosal layer at the center of the anterior and posterior walls of the glandular stomach to make the mucosal layer bulge and separate from the submucosal layer; the mucosa of the gastric antrum is cut off and spread on ordinary filter paper, with the gastric mucosal cavity facing up, and a 1.6mm thick slice of mucosal tissue is cut perpendicular to the mucosal surface with spaced parallel blades; the thick slice is spread on a glass slide, and then the glass slide is placed under an inverted microscope for observation. The picture is displayed on the computer, and a vertical line is drawn on the mucus layer in the picture using software. The pixel value of the straight line is X, and the thickness of the mucus layer is Y, which is converted according to the following formula:

[0069] Y=X×50 / 115 (scale bar: the pixel value of 50 μm is 115).

[0070] Table 4 Effects of the Chinese medicine composition of the present invention on the stomach

[0071]

[0072] The results in Table 4 show that the ginger bamboo shavings in the Chinese medicine composition of the present invention can effectively inhibit the gastrointestinal irritation caused by Hovenia dulcis and the like, and reduce the secretion of gastric acid. Therefore, for the liver and kidney damage caused by alcohol and the adverse effects on the gastrointestinal tract, the Chinese medicine composition of the present invention can reduce gastrointestinal irritation and is suitable for long-term use.

Claims

1. A Chinese medicine composition for preventing or improving alcoholic liver and kidney damage, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in the following weight proportions: 5-50 parts of kudzu root, 5-50 parts of kudzu flower, 6-45 parts of hovenia dulcis, 10-60 parts of seabuckthorn fruit, 10-60 parts of hawthorn, 3-20 parts of ginger bamboo shavings, 6-40 parts of schisandra chinensis, 10-50 parts of rhodiola rosea and 6-50 parts of liquorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in the following weight proportions: 5-40 parts of kudzu root, 5-30 parts of kudzu flower, 6-35 parts of hovenia dulcis, 10-45 parts of seabuckthorn fruit, 10-40 parts of hawthorn, 3-15 parts of ginger bamboo shavings, 6-35 parts of schisandra chinensis, 10-35 parts of rhodiola rosea and 6-30 parts of liquorice.

3. The Chinese medicine composition according to claim 1, characterized in that The invention is prepared from the following Chinese medicinal raw materials in the following weight proportions: 10-30 parts of kudzu root, 10-30 parts of kudzu flower, 10-25 parts of hovenia dulcis, 20-40 parts of seabuckthorn fruit, 15-30 parts of hawthorn, 3-10 parts of ginger bamboo shavings, 9-20 parts of schisandra chinensis, 10-25 parts of rhodiola rosea and 6-20 parts of liquorice.

4. The Chinese medicine composition according to claim 1, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in the following weight proportions: 25 parts of kudzu root, 15 parts of kudzu flower, 15 parts of hovenia dulcis, 30 parts of seabuckthorn fruit, 25 parts of hawthorn, 6 parts of ginger bamboo shavings, 15 parts of schisandra chinensis, 10 parts of rhodiola rosea and 10 parts of liquorice.

5. The Chinese medicine composition according to claim 1, characterized in that: The hawthorn is stir-fried hawthorn, and the licorice is roasted licorice.

6. The Chinese medicine composition according to claim 1, characterized in that: After mixing the various Chinese medicinal materials, add water or alcohol-water solution for extraction.

7. The Chinese medicine composition according to claim 6, characterized in that: The alcohol aqueous solution is selected from 50-90% methanol, ethanol, propanol or butanol solution.

8. A Chinese medicine preparation, characterized in that: The invention comprises the Chinese medicine composition according to any one of claims 1 to 7, and auxiliary materials.

9. The Chinese medicine preparation according to claim 7, characterized in that: The preparation is selected from decoction, powder, granule, tablet, oral liquid, lotion and emulsion.

10. Use of the Chinese medicine composition according to any one of claims 1 to 7 or the Chinese medicine preparation according to any one of claims 8 to 9 in the preparation of a medicament for improving alcoholic liver and kidney damage.

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