Water circulation control method and device for tissue culture bottle filling line and readable storage medium thereof

Abstract

The present invention proposes a water circulation control method and device for a tissue culture bottle filling line and a readable storage medium thereof, including bottle feeding, rough washing, and fine washing stations of specific structures, as well as components such as a water purifier, a main water inlet pump, and a circulating pump. The bottle feeding station linearly inputs the tissue culture bottles into the rough washing station through a material shifting disc and a bottle shifting motor; the rough washing station is provided with an electric rotating frame, a flushing pipe, and a rough washing water tank; the fine washing station has a similar structure and is docked with the rough washing station through a transition track. The water circulation control method includes controlling the water supply of the main water inlet pump according to different stages, and dynamically adjusting the power of the main water inlet pump, the working state of the circulating pump, and the water replenishment strategy in combination with factors such as the rough washing and fine washing time, the ambient temperature, the life of the water purifier, and the energy consumption of the water pump, so as to achieve efficient cleaning of the tissue culture bottles and rational use of water resources, and improve the stability and reliability of the system.

Description

Technical Field

[0001] The present invention relates to the technical field of vessel cleaning equipment, and in particular to a water circulation control method and device for a tissue culture bottle filling line and a readable storage medium thereof. Background Art

[0002] At present, in order to improve the rooting rate and survival rate of seedlings of Chinese medicinal materials and flowers, the culture medium is often injected into the culture bottle for cultivation. During the cultivation process, the culture medium will change from liquid to jelly-like solid agar, which is adsorbed at the bottom of the bottle and generally occupies about 1 / 5 to 1 / 6 of the bottle capacity. Therefore, in the factory-based tissue culture production of seedlings of Chinese medicinal materials, orchids, and flowers, a huge amount of tissue culture bottles are used. In order to reduce costs, glass tissue culture bottles are usually used, which can be reused after cleaning.

[0003] Our institute has applied for patent CN117502244A to disclose a tissue culture container heating, cleaning and filling production line. Although the tissue culture container cleaning station used in it uses automatic cleaning equipment instead of manual cleaning, it still relies on a large amount of water resources, and the water recycling efficiency in the cleaning process is low.

[0004] The most important problem is the lack of refined management of water resources, and the inability to dynamically adjust water use strategies according to various changing factors in actual production (such as cleaning time, ambient temperature, equipment status, etc.). For example, in the water supply control of rough washing and fine washing stations, a fixed water supply mode is mostly used, and the working status of the main water inlet pump and the circulation pump cannot be adjusted in time according to the actual water demand of the station and the changes in the water level of the water tank. At the same time, there is a lack of effective life management and optimization control for the use of water purifiers, which can easily lead to premature damage or overuse of water purifiers, affecting the stability of the overall water circulation system and the water purification effect. Moreover, the traditional cleaning system does not comprehensively consider the impact of ambient temperature on water evaporation, as well as the balance between water pump energy consumption and water use efficiency, making it difficult to achieve efficient, energy-saving, and stable tissue culture bottle cleaning operations.

[0005] Therefore, there is an urgent need for a water circulation control method and device for a tissue culture bottle filling line and a readable storage medium thereof to solve the problems existing in the prior art. Summary of the invention

[0006] The embodiments of the present invention provide a water circulation control method and device for a tissue culture bottle filling line and a readable storage medium thereof, aiming at the problems of water resource waste, poor cleaning effect and the like existing in the current technology.

[0007] The core technology of the present invention is mainly to form a water circulation device with a specific structure of bottle feeding, rough washing, fine washing stations and related equipment, combined with a control method that considers multiple factors such as rough washing and fine washing time, ambient temperature, water purifier life and water pump energy consumption, including dynamic adjustment of water pump parameters and water replenishment strategy and establishment of a water purifier life monitoring model, to achieve efficient cleaning of tissue culture bottles, rational use of water resources and stable operation of the system.

[0008] In a first aspect, the present invention provides a water circulation device for a tissue culture bottle filling line, comprising:

[0009] The bottle feeding station is provided with a material-dispensing disc, a bottle-dispensing motor for driving the material-dispensing disc to rotate, and an input track; the input track guides the tissue culture bottle into a straight line and inputs it into the rough washing station, the material-dispensing disc is arranged on both sides of the width direction of the input track, and each material-dispensing disc is provided with arc-shaped material-dispensing openings arranged at even intervals, and the material-dispensing disc is driven by the bottle-dispensing motor to rotate to push the tissue culture bottle into the rough washing station;

[0010] A rough washing station is provided with a first electric rotating frame, a rough washing station flushing pipe and a rough washing water tank arranged below the first electric rotating frame; the first electric rotating frame is evenly spaced along the circumferential direction. A plurality of cleaning tracks can be rotated to switch the cleaning tracks, each cleaning track is provided with a limit frame for abutting against the step below the mouth of the tissue culture bottle to prevent the tissue culture bottle from being thrown out, and each cleaning track can be docked with the input track when it rotates to the highest point to realize the transfer of the tissue culture bottle; a plurality of nozzles are provided on the rough washing station flushing pipe for flushing the tissue culture bottle on the first electric rotating frame;

[0011] The fine washing station is provided with a second electric rotating frame, a fine washing station flushing pipe, a fine washing water tank arranged below the second electric rotating frame, and an output track; the second electric rotating frame is also provided with a plurality of washing tracks along the circumferential direction and can rotate and switch the washing tracks, each washing track is also provided with a limit frame, and the second electric rotating frame and the first electric rotating frame are docked through a transition track located on the frame to realize the transfer of tissue culture bottles; the fine washing station flushing pipe is also provided with a plurality of nozzles; the output track can be docked with the washing track to facilitate the output of the tissue culture bottles after the fine washing is completed;

[0012] The water purifier is connected to the rough wash water tank through the sewage inlet pipeline and is connected to the water inlet of the circulation pump through the clean water output pipeline;

[0013] The main water inlet pump is connected to the tap water source and is connected to the flushing pipe of the rough washing station and the flushing pipe of the fine washing station through the main water outlet pipe;

[0014] Circulation pump, the water inlet is connected to the water inlet of the fine washing water tank, and the water outlet is connected to the flushing pipe of the rough washing station;

[0015] Among them, the rough wash water tank and the fine wash water tank are both equipped with a water tank overflow alarm sensor and a water tank low level alarm sensor.

[0016] Furthermore, the number of flushing pipes in the rough washing station is one, which is arranged on the top of the first electric rotating frame, and each nozzle is vertically downward and corresponds to a tissue culture bottle;

[0017] The number of flushing pipes in the fine washing station is more than two, which are respectively arranged on the top and side of the second electric rotating frame.

[0018] In a second aspect, the present invention provides a water circulation control method for a tissue culture bottle filling line, which is used for the above-mentioned water circulation device for a tissue culture bottle filling line, and comprises the following steps:

[0019] Obtaining a start signal of the bottle-moving motor, controlling the main water inlet pump according to a preset initial cleaning program, and supplying water to the rough washing station at a preset flow rate; wherein a cleaning track of the first electric rotating rack filled with tissue culture bottles is regarded as a batch;

[0020] As the next batch of tissue culture bottles enter the first electric rotating rack, the first electric rotating rack is continuously rotated intermittently to perform a rough washing operation, while dynamically adjusting the power of the main water inlet pump and monitoring the water level of the rough washing water tank in real time;

[0021] After the number of rinses or the cumulative rinse time of the first batch of tissue culture bottles reaches a preset threshold, the cleaning track where the first batch of tissue culture bottles are located is rotated to the highest position and docked with the cleaning track on the second electric rotating frame. After docking, the bottle shifting motor is turned on, and the tissue culture bottles are fed and transferred at the same time, so that the tissue culture bottles to be washed enter the first electric rotating frame, and the tissue culture bottles after rough washing enter the second electric rotating frame;

[0022] The main water inlet pump is controlled according to the preset fine washing program to supply water to the rough washing station and the fine washing station at the preset flow rate at the same time;

[0023] As the next batch of tissue culture bottles enter the second electric rotating rack, the second electric rotating rack is continuously rotated intermittently to perform fine washing operations, while dynamically adjusting the power of the main water inlet pump and monitoring the water level of the fine washing water tank in real time;

[0024] After the number of times or the cumulative flushing time of the first batch of tissue culture bottles reaches a preset threshold, the cleaning track where the first batch of tissue culture bottles are located is rotated to the highest position, and docked with the cleaning track and the output track on the first electric rotating frame. After docking, the bottle-moving motor is turned on, and the feeding, transfer and output of the tissue culture bottles are performed at the same time, so that the tissue culture bottles to be cleaned enter the first electric rotating frame, the tissue culture bottles after rough washing enter the second electric rotating frame, and the tissue culture bottles after fine washing are output to the output track;

[0025] When the water tank low level alarm sensors of both the fine washing station and the rough washing station are triggered, the circulation pump is turned off;

[0026] When the water tank overflow alarm sensor of the fine wash station is triggered, the circulation pump is turned on to draw water from the fine wash water tank to supply the rough wash spray pipe;

[0027] When the water tank overflow alarm sensor of the rough wash station is triggered, the water purifier is turned on to extract water from the rough wash water tank for purification and then discharged to the circulation pump, and the circulation pump is turned on to supply water to the rough wash spray pipe;

[0028] When the water tank low level alarm sensor at the rough wash station is triggered, the water purifier is turned off;

[0029] When the water tank overflow alarm sensors of the fine washing station and the rough washing station are triggered, the main water inlet pump is turned off and the water purifier and circulation pump are started.

[0030] Furthermore, it also includes a water replenishment method for the main water inlet pump, which specifically includes the following steps:

[0031] Real-time data collection, including the start time t of each cleaning at the rough cleaning station 粗洗开始 and end time t 粗洗结束 , the starting time of each cleaning at the fine cleaning station t 精洗开始 and end time t 精洗结束 , ambient temperature Tm, water volume treated by the water purifier Q 已处理 , the real-time power P of the main water inlet pump and the circulation pump 主 and P 循 ;

[0032] Set initial parameters, including rough wash time threshold T 粗洗阈值 , fine washing time threshold T 精洗阈值 , Ambient temperature threshold T 高 and T 低 、Rated water treatment volume of water purifier Q 额定 , energy consumption threshold E of main water inlet pump and circulation pump 主 and E 循 , time interval threshold T, rough wash water tank water level lower limit threshold L, water replenishment volume V 补 , rough washing time influence coefficient k1, fine washing time influence coefficient k2, temperature compensation coefficient k3, low temperature adjustment coefficient k4, remaining life warning coefficient k5, water purifier life influence adjustment value k7, main water inlet pump energy consumption adjustment coefficient k8, circulation pump energy consumption adjustment coefficient k9;

[0033] Calculate the actual time T for each rough cleaning operation 粗实际 and the actual time T of the fine washing operation 精实际 ;

[0034] When T 粗实际 >T 粗洗阈值 When the time interval threshold T is adjusted to T 1 =T-k1(T 粗实际 -T粗洗阈值 ) to speed up the frequency of water replenishment;

[0035] When T 精实际 >T 精洗阈值 When the water level in the fine washing station water tank is lower than the preset proportion of its full water level, the main water inlet pump is immediately started to partially replenish water, and the time interval threshold T is adjusted to T 2 =T-k2(T 精实际 -T 精洗阈值 );

[0036] When Tm>T 高 When the temperature compensation coefficient K is calculated T =k3(Tm-T 高 ), adjust the time interval threshold T to T 3 =T / (1+K T ), and increase the water replenishment V 补 =V 补 (1+K T ) to compensate for the water loss caused by high temperature evaporation;

[0037] When Tm<T 低 When the water supply is sufficient, the operating frequency of the main water inlet pump and the circulation pump is reduced to reduce energy consumption, and the time interval threshold T is adjusted to T 4 =T(1+k4(T 低 -Tm));

[0038] Preset amount per treatment Q 监测 water, update the treated water volume Q 已处理 =Q 已处理 +Q 监测 ;

[0039] When Q 已处理 >(1-k5)Q 额定 When the life span of the water purifier is over, a warning signal is issued, and after the warning, the proportion of circulating water usage is reduced, the flow rate of the circulating pump is reduced to (1-k6) times of the original, and the replenishment frequency of the main water inlet pump is increased, and the time interval threshold T is updated to T 5 =T-k7, to extend the life of the water purifier;

[0040] Calculate the average energy consumption E of the main water inlet pump and circulation pump within a certain period of time 主平均 and E 循平均 ;

[0041] When E 主平均 >E 主 When the power of the main water inlet pump is reduced, the time interval threshold T is updated to T 6 =T+k8, to reduce unnecessary water replenishment operations;

[0042] When E 循平均 >E 循 When the water demand is high, reduce the flow rate of the circulation pump to (1-k9) times of the original value, and adjust its running time according to the actual water demand;

[0043] After the operation is stable or the preset cycle time is reached, the final time interval threshold T is obtained. final .

[0044] Furthermore, taking T 1 -T 6 The minimum value among them is taken as the final time interval threshold T final .

[0045] Furthermore, the water replenishment method also includes:

[0046] When the water tank overflow alarm sensors of the fine washing station and the rough washing station are triggered, the main water inlet pump is turned off, the water purifier and the circulation pump are started, and the circulating sewage time t is recorded at the same time;

[0047] When one of the following conditions is met, turn on the main water inlet pump to replenish water:

[0048] Circulating sewage time t reaches T final ;

[0049] The water level of the rough wash water tank detects the lower limit threshold value L of the rough wash water tank water level;

[0050] When replenishing water, the final replenishment water volume V 补 Perform water replenishment operation to ensure that the water level in the rough wash water tank returns to the appropriate level.

[0051] Furthermore, the value of the time interval threshold T is based on water consumption estimation, empirical value or experimental value.

[0052] Furthermore, it also includes a water purifier life monitoring model, through which the remaining life of the water purifier is predicted. The method for establishing the water purifier life monitoring model records the working hours of the water purifier, uses a flow sensor to monitor the cumulative treated water volume, and uses a variety of water quality sensors to monitor water quality changes. Life prediction models are constructed and weights are assigned respectively, and a comprehensive model is constructed to evaluate the remaining life in real time, and a warning signal is issued according to the remaining life warning value and the model parameters are continuously optimized.

[0053] In a third aspect, the present invention provides an electronic device including a memory and a processor, wherein the memory stores a computer program, and the processor is configured to run the computer program to execute the above-mentioned tissue culture bottle filling line water circulation control method.

[0054] In a fourth aspect, the present invention provides a readable storage medium, in which a computer program is stored. The computer program includes a program code for controlling a process to execute the process. The process includes the water circulation control method for the tissue culture bottle filling line according to the above-mentioned method.

[0055] The main contributions and innovations of the present invention are as follows:

[0056] 1) Efficient use of water resources: The present invention realizes accurate allocation of water resources by real-time monitoring of the water usage of the rough washing station and the fine washing station, including factors such as cleaning time and water level changes, and dynamically adjusting the working status of the main water inlet pump and the circulation pump. For example, according to the changes in the rough washing time and the fine washing time, the flow rate of the main water inlet pump is adjusted in time to avoid waste of water resources. When the water level in the water tank of the fine washing station overflows, the circulation pump supplies water to the rough washing station, which improves the utilization rate of circulating water and greatly reduces the consumption of tap water compared to the existing technology.

[0057] 2) Improved equipment operation stability: Taking into account the life of the water purifier, a life monitoring model was established. By monitoring the working hours, water volume and water quality changes, the circulating water usage ratio and the main water pump replenishment frequency were adjusted in a timely manner, which effectively extended the life of the water purifier and reduced the risk of system downtime caused by water purifier failure. At the same time, the water pump operation frequency and water replenishment strategy were adjusted according to the ambient temperature to ensure the stable operation of the equipment under different environmental conditions and reduce the adverse effects of temperature changes on the equipment.

[0058] 3) Cleaning quality assurance: Precisely control the cleaning process of the rough and fine washing stations, including the transfer of tissue culture bottles at each station, the intermittent rotation of the rotating rack, and the flushing operation of the flushing tube, to ensure that all parts of the tissue culture bottles can be fully cleaned. In addition, by dynamically adjusting the water pump parameters, it is guaranteed that there is a stable water pressure and water supply under different working conditions (such as different production tasks, different water quality, etc.), which helps to maintain the consistency of cleaning quality and improve the cleaning qualification rate of tissue culture bottles.

[0059] 4) Energy saving and consumption reduction: Under the premise of meeting the cleaning needs, the energy consumption of the main water inlet pump and the circulation pump is monitored and analyzed in real time, and the working parameters of the water pump (such as flow rate, head, etc.) are adjusted to reduce the energy consumption of the water pump. For example, when it is found that the energy consumption of the water pump is too high, the control strategy is optimized to make the water pump work in a more energy-saving state, thereby reducing the energy consumption of the entire water circulation system and reducing production costs.

[0060] 5) Intelligent control: The control method of the present invention takes into account a variety of factors and realizes intelligent control of the entire water circulation system through algorithms. From data collection, parameter calculation to equipment control decision-making, each link is interrelated and works together, and can automatically adapt to different production conditions and environmental changes. There is no need for frequent manual intervention, which improves production efficiency and reduces labor costs, making the water circulation control of the tissue culture bottle filling line more scientific and efficient.

[0061] The details of one or more embodiments of the invention are set forth in the following drawings and description so that other features, objects, and advantages of the invention are more readily apparent. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0063] Figure 1 is a three-dimensional diagram of a water circulation device for a tissue culture bottle filling line according to an embodiment of the present invention;

[0064] Figure 2 is a front view of a water circulation device for a tissue culture bottle filling line according to an embodiment of the present invention;

[0065] Figure 3 yes Figure 1 A magnified view of middle;

[0066] Figure 4 yes Figure 1 Enlarged view of middle B;

[0067] Figure 5 yes Figure 1 Enlarged view of middle C;

[0068] Figure 6 4 is a schematic diagram of the hardware structure of an electronic device according to an embodiment of the present invention.

[0069] In the figure, 1. Main water inlet pump; 2. Circulation pump; 3. Main water inlet main pipeline; 4. Main water outlet pipeline; 5. Sewage inlet pipeline; 6. Clean water outlet pipeline; 7. Rough wash station circulating water supply pipeline; 8. Water purifier; 9. Fine wash water tank outlet; 10. Fine wash water tank; 11. Water tank overflow alarm sensor; 12. Fine wash station side flushing pipe; 13. Fine wash station upper flushing pipe; 14. Low level alarm sensor; 15. Circulating water inlet ; 16. Flushing pipe of the rough washing station; 17. Main water inlet pipe of the fine washing station; 18. Tap water inlet; 19. Rough washing water tank; 20. Transition track; 21. Material transfer disc; 22. First electric rotating frame; 23. Second electric rotating frame; 24. Input track; 25. Cleaning track; 26. Limiting frame; 27. Output track; 402. Processor; 404. Memory; 406. Transmission equipment; 408. Input and output equipment. DETAILED DESCRIPTION

[0070] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the following exemplary embodiments do not represent all implementations consistent with one or more embodiments of this specification. Instead, they are merely examples of devices and methods consistent with some aspects of one or more embodiments of this specification as detailed in the appended claims.

[0071] It should be noted that: in other embodiments, the steps of the corresponding method are not necessarily performed in the order shown and described in this specification. In some other embodiments, the steps included in the method may be more or less than those described in this specification. In addition, a single step described in this specification may be decomposed into multiple steps for description in other embodiments; and multiple steps described in this specification may be combined into a single step for description in other embodiments.

[0072] Embodiment 1

[0073] The present invention aims to propose a water circulation device for a tissue culture bottle filling line, specifically, referring to Figure 1-Figure 5 As shown, including:

[0074] The bottle feeding station is provided with a material-dispensing disc 21, a bottle-dispensing motor for driving the material-dispensing disc 21 to rotate, and an input track 24; the input track 24 guides the tissue culture bottle into a straight line and inputs it into the rough washing station, the material-dispensing discs 21 are arranged on both sides of the width direction of the input track 24, and each material-dispensing disc 21 is provided with arc-shaped material-dispensing openings arranged at uniform intervals, and the material-dispensing disc 21 is driven by the bottle-dispensing motor to rotate to push the tissue culture bottle into the rough washing station;

[0075] The rough washing station is provided with a first electric rotating frame 22, a rough washing station flushing pipe and a rough washing water tank 19 arranged below the first electric rotating frame 22; the first electric rotating frame 22 is evenly spaced along the circumferential direction. A plurality of cleaning tracks 25 can be rotated to switch the cleaning tracks 25, each cleaning track 25 is provided with a limit frame 26 for abutting against the step below the mouth of the tissue culture bottle to prevent the tissue culture bottle from being thrown out, and each cleaning track 25 can be docked with the input track 24 when it rotates to the highest point to realize the transfer of the tissue culture bottle; a plurality of nozzles are provided on the rough washing station flushing pipe for flushing the tissue culture bottles on the first electric rotating frame 22;

[0076] In this embodiment, there is one flushing pipe in the rough washing station, which is arranged on the top of the first electric rotating frame 22, and each nozzle is vertically downward and corresponds to a tissue culture bottle. The first electric rotating frame 22 and the second electric rotating frame 23 are both driven by servo motors, and their driving principle and structure are not described here, which are common means in the mechanical field.

[0077] The fine washing station is provided with a second electric rotating frame 23, a fine washing station flushing pipe, a fine washing water tank 10 arranged below the second electric rotating frame 23, and an output track 27; the second electric rotating frame 23 is also provided with a plurality of cleaning tracks 25 along the circumferential direction and can rotate and switch the cleaning tracks 25, each cleaning track 25 is also provided with a limit frame 26, and the second electric rotating frame 23 and the first electric rotating frame 22 are connected to each other through a transition track 20 located on the frame to realize the transfer of tissue culture bottles; the fine washing station flushing pipe is also provided with a plurality of nozzles; the output track 27 can be connected with the cleaning track 25 to facilitate the output of the tissue culture bottles after the fine washing is completed;

[0078] In this embodiment, there are more than two fine washing station flushing pipes, which are respectively arranged on the top and side of the second electric rotating frame 23. In this way, it can ensure that it has a better flushing effect, and even the fine washing station flushing pipe located on the side is facing the inside of the tissue culture bottle, because this process only flushes the inside of the bottle, and the cleaning of the outside of the bottle is not the work of this process.

[0079] The water purifier 8 is connected to the rough wash water tank 19 through the sewage inlet pipe 5 and is connected to the water inlet of the circulation pump 2 through the clean water output pipe 6;

[0080] The main water inlet pump 1 is connected to the tap water source and is connected to the flushing pipe of the rough washing station and the flushing pipe of the fine washing station through the main water outlet pipe 4;

[0081] The circulating pump 2 has a water inlet connected to the water inlet of the fine washing water tank 10, and a water outlet connected to the flushing pipe of the rough washing station;

[0082] Wherein, the rough wash water tank 19 and the fine wash water tank 10 are both provided with a water tank overflow alarm sensor 11 and a water tank low level alarm sensor 14 .

[0083] In this embodiment, the first electric rotating frame 22 and the second electric rotating frame 23 are both driven by servo motors, and the rough wash water tank 19 and the fine wash water tank 10 are both provided with a discharge valve and a valve for water inlet switch. Each brush head of the rough wash station flushing pipe 16 and the fine wash station flushing pipe is selected to be a model that can be opened and closed individually and the spray angle can be adjusted, and the rough wash station flushing pipe 16 and the fine wash station flushing pipe are both provided with solenoid valves to control the water inlet and switch.

[0084] like Figure 1 and Figure 2 In the figure, the left side is the fine washing station, and the right side is the rough washing station; both the left and right stations need to be filled with water to rinse the tissue culture bottles, the fine washing station is arranged with two groups of flushing pipes (fine washing station side flushing pipe 12 and fine washing station upper flushing pipe 13), and the rough washing station is arranged with one group of flushing pipes (rough washing station flushing pipe 16); the rough washing station flushing pipe 16 has two water inlets, the left side is the circulating water inlet 15, and the right side is the tap water inlet 18. Water is pumped from the tap water main line through the main water inlet pump 1 through the main water inlet main pipe 3, and then supplied to the rough washing station through the main water outlet pipe 4, and then supplied to the fine washing station for use through the main water inlet pipe 17 of the fine washing station connected to the main water outlet pipe 4. Since the sewage after flushing in the fine washing station is relatively clean, the fine washing tank outlet 9 of the fine washing water tank 10 below the fine washing station is directly connected to the water inlet of the circulation pump 2, and is supplied to the flushing pipe 16 of the rough washing station for use through the rough washing station circulating water supply pipe 7 of the circulation pump 2; and the water after cleaning in the rough washing station is relatively turbid, the rough washing water tank 19 below the rough washing station is connected to the sewage inlet pipe 5 of the water purifier 8, and is filtered by the water purifier 8 and discharged from the clean water output pipe 6 of the water purifier 8. Since the clean water output pipe 6 of the water purifier 8 is connected to the water inlet of the circulation pump 2, the clean water is again supplied to the flushing pipe 16 of the rough washing station for use through the circulation pump 2. A set of ultrasonic water level sensors (can also be two sets, water tank overflow alarm sensor 11 and water tank low level alarm sensor 14) are arranged directly above the rough wash water tank 19 to monitor the water volume of the rough wash water tank. When the water volume exceeds the set value, the water purifier 8 starts to work and pumps water from the rough wash water tank for filtration. A set of ultrasonic water level sensors (water tank overflow alarm sensor 11 and water tank low level alarm sensor 14) are arranged on both sides directly above the fine wash station water tank to monitor the high point and low point of the water level respectively. When the water level of the fine wash station water tank reaches the high point, the circulation pump 2 starts to work and pumps water from the fine wash water tank 10. When the water level of the fine wash water tank 10 is lower than the low point, the circulation pump 2 stops working.

[0085] Embodiment 2

[0086] Based on the same concept, the present invention also proposes a water circulation control method for a tissue culture bottle filling line, which is used in a water circulation device for a tissue culture bottle filling line in Embodiment 1, and comprises the following steps:

[0087] Obtain a start signal of the bottle-moving motor, control the main water inlet pump 1 according to a preset initial cleaning program, and supply water to the rough cleaning station at a preset flow rate; wherein, a cleaning track 25 of the first electric rotating frame 22 filled with tissue culture bottles is regarded as a batch;

[0088] As the next batch of tissue culture bottles enter the first electric rotating frame 22, the first electric rotating frame 22 is continuously and intermittently rotated to perform a rough washing operation, while dynamically adjusting the power of the main water inlet pump 1 and monitoring the water level of the rough washing water tank 19 in real time;

[0089] After the number of times or the cumulative flushing time of the first batch of tissue culture bottles reaches a preset threshold, the cleaning track 25 where the first batch of tissue culture bottles are located is rotated to the highest position and docked with the cleaning track 25 on the second electric rotating frame 23. After docking, the bottle shifting motor is turned on, and the feeding and transfer of the tissue culture bottles are performed at the same time, so that the tissue culture bottles to be cleaned enter the first electric rotating frame 22, and the tissue culture bottles after the rough washing are entered into the second electric rotating frame 23;

[0090] According to the preset fine washing program, the main water inlet pump 1 is controlled to supply water to the rough washing station and the fine washing station at the preset flow rate;

[0091] As the next batch of tissue culture bottles enter the second electric rotating frame 23, the second electric rotating frame 23 is continuously and intermittently rotated to perform a fine washing operation, while dynamically adjusting the power of the main water inlet pump 1 and monitoring the water level of the fine washing water tank 10 in real time;

[0092] After the number of times or the cumulative flushing time of the first batch of tissue culture bottles reaches a preset threshold, the cleaning track 25 where the first batch of tissue culture bottles are located is rotated to the highest position, and docked with the cleaning track 25 and the output track 27 on the first electric rotating frame 22. After docking, the bottle-moving motor is turned on, and the feeding, transfer and output of the tissue culture bottles are performed at the same time, so that the tissue culture bottles to be cleaned enter the first electric rotating frame 22, the tissue culture bottles after rough washing enter the second electric rotating frame 23, and the tissue culture bottles after fine washing are output to the output track 27;

[0093] When the water tank low level alarm sensors 14 of both the fine washing station and the rough washing station are triggered, the circulation pump 2 is turned off;

[0094] When the water tank overflow alarm sensor 11 of the fine wash station is triggered, the circulation pump 2 is turned on to draw water from the fine wash water tank 10 to supply the rough wash spray pipe;

[0095] When the water tank overflow alarm sensor 11 of the rough wash station is triggered, the water purifier 8 is turned on to extract water from the rough wash water tank 19 for purification and then discharged to supply the circulating pump 2, and the circulating pump 2 is turned on to supply water to the rough wash spray pipe;

[0096] When the water tank low level alarm sensor 14 of the rough washing station is triggered, the water purifier 8 is turned off;

[0097] When the water tank overflow alarm sensors 11 of the fine washing station and the rough washing station are triggered, the main water inlet pump 1 is turned off, and the water purifier 8 and the circulation pump 2 are started.

[0098] In this embodiment, the water replenishment method of the main water inlet pump 1 specifically includes the following steps:

[0099] Real-time data collection, including the start time t of each cleaning at the rough cleaning station 粗洗开始 and end time t 粗洗结束 , the starting time of each cleaning at the fine cleaning station t 精洗开始 and end time t 精洗结束 , ambient temperature Tm, water volume treated by water purifier 8 Q 已处理 , the real-time power P of the main water inlet pump 1 and the circulation pump 2 主 and P 循 ;

[0100] Set initial parameters, including rough wash time threshold T 粗洗阈值 , fine washing time threshold T 精洗阈值 , Ambient temperature threshold T 高 and T 低 、Water purifier 8 Rated water treatment volume Q 额定 , energy consumption threshold E of main water inlet pump 1 and circulation pump 2 主 and E 循 , time interval threshold T, rough wash water tank 19 water level lower limit threshold L, water replenishment volume V 补 , rough washing time influence coefficient k1, fine washing time influence coefficient k2, temperature compensation coefficient k3, low temperature adjustment coefficient k4, remaining life warning coefficient k5, water purifier 8 life influence adjustment value k7, main water inlet pump 1 energy consumption adjustment coefficient k8, circulation pump 2 energy consumption adjustment coefficient k9;

[0101] Calculate the actual time T for each rough cleaning operation 粗实际 and the actual time T of the fine washing operation 精实际 ;

[0102] When T 粗实际 >T 粗洗阈值 When the time interval threshold T is adjusted to T 1 =T-k1(T 粗实际 -T 粗洗阈值 ) to speed up the frequency of water replenishment;

[0103] When T 精实际 >T 精洗阈值 When the water level of the fine washing station water tank is lower than the preset proportion of its full water level, the main water inlet pump 1 is immediately started to partially replenish water, and the time interval threshold T is adjusted to T 2 =T-k2(T 精实际 -T 精洗阈值 );

[0104] When Tm>T 高 When the temperature compensation coefficient K is calculated T =k3(Tm-T 高 ), adjust the time interval threshold T to T 3 =T / (1+K T ), and increase the water replenishment V 补 =V 补 (1+K T ) to compensate for the water loss caused by high temperature evaporation;

[0105] When Tm<T 低 When the basic water demand is met, the operating frequency of the main water inlet pump 1 and the circulation pump 2 is reduced to reduce energy consumption, and the time interval threshold T is adjusted to T 4 =T(1+k4(T 低 -Tm));

[0106] Preset amount per treatment Q 监测 water, update the treated water volume Q 已处理 =Q 已处理 +Q 监测 ;

[0107] When Q 已处理 >(1-k5)Q 额定 When the life span of water purifier 8 is reached, a warning signal is issued, and after the warning, the proportion of circulating water usage is reduced, the flow rate of circulating pump 2 is reduced to (1-k6) times of the original, and the replenishment frequency of main water inlet pump 1 is increased, and the time interval threshold T is updated to T 5 =T-k7, to extend the life of the water purifier 8;

[0108] Calculate the average energy consumption E of the main water inlet pump 1 and the circulation pump 2 within a certain period of time 主平均 and E 循平均 ;

[0109] When E 主平均 >E 主 When the power of the main water inlet pump 1 is reduced, the time interval threshold T is updated to T 6 =T+k8, to reduce unnecessary water replenishment operations;

[0110] When E 循平均 >E 循 When the water demand is high, reduce the flow rate of circulating pump 2 to (1-k9) times of the original value, and adjust its running time according to the actual water demand;

[0111] After the operation is stable or the preset cycle time is reached, the final time interval threshold T is obtained. final ; Among them, take T 1 -T 6The minimum value among them is taken as the final time interval threshold T final ;

[0112] When the water tank overflow alarm sensors 11 of both the fine washing station and the rough washing station are triggered, the main water inlet pump 1 is turned off, the water purifier 8 and the circulation pump 2 are started, and the circulating sewage time t is recorded at the same time;

[0113] When one of the following conditions is met, the main water inlet pump 1 is turned on to replenish water:

[0114] Circulating sewage time t reaches T final ;

[0115] The water level of the rough wash water tank 19 detects the lower limit threshold value L of the water level of the rough wash water tank 19;

[0116] When replenishing water, the final replenishment water volume V 补 Perform water replenishment operations to ensure that the water level in the rough wash water tank 19 returns to an appropriate level. At the same time, continue to monitor various data and dynamically adjust algorithm parameters to adapt to changing operating conditions.

[0117] In this way, through such a water replenishment algorithm that comprehensively considers multiple factors and corrects the symbol conflict, the water circulation system of the tissue culture bottle filling line can be controlled more intelligently, and the rational use of water resources, stable operation of equipment and effective control of energy consumption can be achieved while ensuring production efficiency and cleaning quality. In practical applications, it is also necessary to optimize and adjust the coefficients and thresholds in the algorithm according to the specific equipment and production environment to achieve the best performance.

[0118] Preferably, the value of the time interval threshold T is based on water consumption estimation, empirical value or experimental value. The following are some preferred methods for determining the appropriate value of the time interval threshold T:

[0119] 1) Estimation based on water consumption: First, according to the cleaning process and standard water consumption of tissue culture bottles, calculate the total water consumption Q of the rough washing station and the fine washing station within a certain period of time (such as one hour) under normal production conditions. 总 Then, consider the capacity V of the water tank 水箱 , through the formula:

[0120] T=V 水箱 / Q 总 Preliminarily estimate the theoretical value of a time interval threshold T. For example, if the total water consumption per hour is calculated to be 100L and the water tank capacity is 500L, then preliminarily estimate T=5 hours (this is just a simple example).

[0121] 2) Refer to the experience of similar equipment: Investigate the water circulation systems of the same or similar tissue culture bottle filling lines on the market to obtain their experience data on the time interval threshold setting. Understand the value ranges of these equipment under different production scales and working conditions as a reference for the setting of this system. If the time interval threshold of other similar equipment is usually set between 3-6 hours during normal operation, then the T value range of this system can be initially set near this range, and then adjusted according to the characteristics of its own equipment.

[0122] 3) Single factor experiment: Conduct a single factor experiment on a simulation experiment platform or an actual production line to specifically test the impact of the time interval threshold T on system operation. Set different T values, such as 2 hours, 4 hours, 6 hours, 8 hours, etc., and run the equipment for a period of time (such as one week) while keeping other conditions unchanged. Record the following data: total tap water consumption, circulating water utilization rate, tissue culture bottle cleaning quality (by detecting indicators such as the amount of residual dirt or microbial content in the tissue culture bottle after cleaning), and the number of equipment failures (especially equipment related to the water circulation system, such as water pumps, sensors, etc.). Analyze these data and observe the performance of the system under different T values. For example, when the T value is small, tap water consumption may increase, but the circulating water utilization rate may be high, and the cleaning quality may be relatively stable; when the T value is large, problems such as large fluctuations in the water tank water level and reduced cleaning quality may occur.

[0123] 4) Multi-factor experiment and response surface analysis: If it is found in actual operation that the time interval threshold T interacts with other factors (such as ambient temperature, production task volume, etc.) and affects system performance, a multi-factor experiment is conducted. For example, multiple combined experimental conditions are set in combination with different ambient temperatures (low temperature, normal temperature, high temperature) and production task volumes (low, medium, and high), and different T values ​​are tested under each condition. Through response surface analysis and other methods, a mathematical model between the T value and system performance indicators (such as water cost, cleaning quality, equipment maintenance cost, etc.) is established to find the combination of T values ​​that optimizes the overall performance of the system. Although this method is complex, it can more accurately determine the appropriate value of T, and is especially suitable for situations with high system performance requirements and complex working conditions.

[0124] 5) In the actual production process, the system's operating status and related data, including water level changes, water quality, equipment energy consumption, etc., are continuously monitored in real time. Even after the value of a time interval threshold T is initially determined, it cannot remain unchanged. According to the real-time monitoring data, determine whether the current T value is still appropriate. For example, if it is found that the water level in the water tank is often close to the lower threshold for a period of time, or the circulating water quality decreases rapidly, it may mean that the T value is too large and needs to be appropriately reduced; conversely, if the tap water consumption is too high and the circulating water utilization rate is low, the T value may need to be increased. Through this dynamic adjustment mechanism, the value of the time interval threshold T is continuously optimized so that it always adapts to the changing needs of actual production and ensures efficient and stable operation of the system.

[0125] Preferably, the life warning signal of the water purifier 8 is issued through the life monitoring model of the water purifier 8, and the remaining life of the water purifier 8 is predicted by the life monitoring model of the water purifier 8. The method for establishing the life monitoring model of the water purifier 8 records the working time of the water purifier 8, uses a flow sensor to monitor the cumulative amount of water treated, and uses a variety of water quality sensors to monitor water quality changes. Life prediction models are constructed and weights are assigned respectively, and a comprehensive model is constructed to evaluate the remaining life in real time, and a warning signal is issued according to the remaining life warning value and the model parameters are continuously optimized. Specifically, the following steps are included:

[0126] 1) Working hours monitoring and analysis

[0127] Data recording: A special timer is provided for the water purifier 8 to accurately record the cumulative working time from the beginning of use to the current moment. 累计 . Record and store working hours data at certain time intervals (such as daily or weekly) for subsequent analysis.

[0128] Life prediction model construction: Based on a large number of historical data of similar water purifiers 8 (including information such as failure probability under different working hours), a relationship model between working hours and remaining life can be constructed. For example, a linear regression model or an exponential decay model can be used. Assuming that a linear regression model is used, the remaining life L 剩余 and the accumulated working time t 累计 The relationship is:

[0129] L 剩余 =a-bt 累计 (where a and b are coefficients obtained by fitting historical data.) By performing regression analysis on multiple sets of data on known remaining life and corresponding working hours, the values ​​of a and b are determined, thereby obtaining a working hour life prediction model for the water purifier 8 of this system.

[0130] 2) Monitoring and analysis of treated water volume

[0131] Flow sensor installation and data collection: Install a high-precision flow sensor at the water inlet or outlet of the water purifier 8 to monitor the water flow q(t) passing through the water purifier 8 in real time. Integrate the flow data to obtain the cumulative treated water volume . The accumulated water volume data is also recorded and stored at certain time intervals.

[0132] Establishment of the water treatment life prediction model: Based on the rated water treatment volume Q of the water purifier 8 额定 And the actual cumulative treated water volume Q 累计 , establish a remaining life prediction model based on the amount of treated water. For example, using a simple proportional model, assuming that the remaining life ratio r=(Q 额定 -Q 累计 ) / Q 额 , then the remaining life L 剩余 =r*L 设计 (L 设计 Designed for the total life of the water purifier 8).

[0133] 3) Monitoring and analysis of water quality changes

[0134] Water quality sensor configuration and data monitoring: Install a variety of water quality sensors, such as turbidity sensors, conductivity sensors, pH sensors, etc., to monitor the water quality parameters of the water purifier 8 inlet and outlet in real time. Calculate water purification e 效率 =(C 进 -C 出 ) / C 进 (C 进 is the influent water quality parameter value, C 出 is the water quality parameter value of the outlet water), and regularly record the water purification efficiency data. Or use the built-in water quality sensor of the water purifier 8 to perform water quality detection.

[0135] Water quality life prediction model construction: As the water purifier8 is used for a longer time and the amount of water treated increases, the water quality purification efficiency will gradually decrease. By analyzing a large amount of historical data, a relationship model between water quality purification efficiency and remaining life is established. For example, when the water quality purification efficiency drops to a certain threshold value e 阈值 When L is reached, it can be considered that the life of the water purifier 8 is about to end. A nonlinear model (such as a logarithmic model or a quadratic function model, etc.) can be used to describe the trend of water purification efficiency changing with time or water volume, and then predict the remaining life. For example, suppose L 剩余 =f(e), where f(e) is the function of water purification efficiency obtained by fitting historical data.

[0136] 4) Comprehensive model establishment and remaining life assessment

[0137] Weight allocation and comprehensive model construction: Comprehensively consider the impact of working hours, water treatment volume and water quality changes on the life of the water purifier 8. Assign different weights w1, w2 and w3 (w1+w2+w3=1) to these three factors according to actual conditions. For example, after experiments and analysis, it was found that the amount of water treated has a greater impact on the life, and the weight can be set to w2=0.5; the working hours and water quality changes have relatively small effects, and the weights are set to w1=0.3 and w3=0.2 respectively. Construct a comprehensive remaining life prediction model:

[0138] L 剩余 =w1*L 剩余1 +w2*L 剩余2 +w3*L 剩余3

[0139] (L 剩余1 , L 剩余2 and L 剩余3 are the remaining lifespan predicted based on working hours, water volume treated and water quality changes respectively).

[0140] Real-time evaluation and early warning: During the operation of the system, the data on working hours, water volume and water quality changes are collected in real time and substituted into the comprehensive model to calculate the remaining life. When the remaining life is lower than the set early warning value (such as the remaining life is less than 10% of the total design life), an early warning signal is issued to remind the operator to maintain or replace the water purifier in time8 to ensure the stable operation of the water circulation system and the water purification effect. At the same time, the parameters in the model (such as weight coefficients, etc.) are continuously optimized according to the actual operation data to improve the accuracy of the remaining life assessment.

[0141] Embodiment 3

[0142] This embodiment also provides an electronic device, referring to Figure 6 , comprises a memory 404 and a processor 402, wherein the memory 404 stores a computer program, and the processor 402 is configured to run the computer program to execute the steps in any of the above method embodiments.

[0143] Specifically, the processor 402 may include a central processing unit (CPU), or an application specific integrated circuit (ASIC), or may be configured to implement one or more integrated circuits of the embodiment of the present invention.

[0144] Among them, the memory 404 may include a large capacity memory 404 for data or instructions. For example, but not limitation, the memory 404 may include a hard disk drive (HDD), a floppy disk drive, a solid state drive (SSD), a flash memory, an optical disk, a magneto-optical disk, a tape, or a universal serial bus (USB) drive, or a combination of two or more of these. In appropriate cases, the memory 404 may include a removable or non-removable (or fixed) medium. In appropriate cases, the memory 404 may be inside or outside the data processing device. In a specific embodiment, the memory 404 is a non-volatile memory. In a specific embodiment, the memory 404 includes a read-only memory (ROM) and a random access memory (RAM). Where appropriate, the ROM may be a mask-programmed ROM, a programmable ROM (Programmable Read-Only Memory, PROM for short), an erasable PROM (Erasable Programmable Read-Only Memory, EPROM for short), an electrically erasable PROM (Electrically Erasable Programmable Read-Only Memory, EEPROM for short), an electrically alterable ROM (Electrically Alterable Read-Only Memory, EAROM for short) or a flash memory (FLASH) or a combination of two or more of these. In appropriate circumstances, the RAM may be a static random access memory (SRAM) or a dynamic random access memory (DRAM), wherein the DRAM may be a fast page mode dynamic random access memory 404 (FPMDRAM), an extended data output dynamic random access memory (EDODRAM), a synchronous dynamic random access memory (SDRAM), etc.

[0145] The memory 404 may be used to store or cache various data files required for processing and / or communication, as well as possible computer program instructions executed by the processor 402 .

[0146] The processor 402 reads and executes the computer program instructions stored in the memory 404 to implement any one of the water circulation control methods for the tissue culture bottle filling line in the above embodiments.

[0147] Optionally, the electronic device may further include a transmission device 406 and an input / output device 408 , wherein the transmission device 406 is connected to the processor 402 , and the input / output device 408 is connected to the processor 402 .

[0148] The transmission device 406 can be used to receive or send data via a network. Specific examples of the above-mentioned network may include a wired or wireless network provided by a communication provider of the electronic device. In one example, the transmission device includes a network adapter (Network Interface Controller, referred to as NIC), which can be connected to other network devices through a base station so as to communicate with the Internet. In one example, the transmission device 406 can be a radio frequency (Radio Frequency, referred to as RF) module, which is used to communicate with the Internet wirelessly.

[0149] Input / output devices 408 are used to input or output information.

[0150] Embodiment 4

[0151] This embodiment also provides a readable storage medium, in which a computer program is stored. The computer program includes a program code for controlling a process to execute the process. The process includes the water circulation control method for the tissue culture bottle filling line according to the first embodiment.

[0152] It should be noted that the specific examples in this embodiment can refer to the examples described in the above embodiments and optional implementation modes, and this embodiment will not be described in detail here.

[0153] In general, various embodiments may be implemented in hardware or dedicated circuits, software, logic, or any combination thereof. Some aspects of the invention may be implemented in hardware, while other aspects may be implemented in firmware or software executed by a controller, microprocessor, or other computing device, but the invention is not limited thereto. Although various aspects of the invention may be shown and described as block diagrams, flow charts, or using some other graphical representation, it should be understood that, as non-limiting examples, the boxes, devices, systems, techniques, or methods described herein may be implemented in hardware, software, firmware, dedicated circuits or logic, general-purpose hardware or controllers or other computing devices, or some combination thereof.

[0154] Embodiments of the present invention can be implemented by computer software, which is executable by a data processor of a mobile device, such as in a processor entity, or implemented by hardware, or implemented by a combination of software and hardware. Computer software or programs (also referred to as program products) including software routines, applets and / or macros can be stored in any device readable data storage medium, and they include program instructions for performing specific tasks. Computer program products can include one or more computer executable components configured to perform embodiments when the program is running. One or more computer executable components can be at least one software code or a part thereof. In addition, at this point, it should be noted that any box of the logic flow can represent a program step, or interconnected logic circuits, boxes and functions, or a combination of program steps and logic circuits, boxes and functions. Software can be stored in physical media such as memory chips or storage blocks implemented in processors, magnetic media such as hard disks or floppy disks, and optical media such as, for example, DVDs and data variants thereof, CDs. Physical media are non-transient media.

[0155] Those skilled in the art should understand that the technical features of the above embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0156] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that, for those of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.

Claims

1. A water circulation device for a tissue culture bottle filling line, characterized in that: include: The bottle feeding station is provided with a material-dispensing disc, a bottle-dispensing motor for driving the material-dispensing disc to rotate, and an input track; the input track guides the tissue culture bottle into a straight line and inputs it into the rough washing station, the material-dispensing disc is arranged on both sides of the width direction of the input track, and each of the material-dispensing discs is provided with arc-shaped material-dispensing openings arranged at even intervals, and the material-dispensing disc is driven by the bottle-dispensing motor to rotate to push the tissue culture bottle into the rough washing station; A rough washing station is provided with a first electric rotating frame, a rough washing station flushing pipe and a rough washing water tank arranged below the first electric rotating frame; the first electric rotating frame is evenly spaced along the circumferential direction. A plurality of cleaning tracks can be rotated to switch the cleaning tracks, each cleaning track is provided with a limit frame for abutting against the step below the mouth of the tissue culture bottle to prevent the tissue culture bottle from being thrown out, and each cleaning track can be docked with the input track when it rotates to the highest point to realize the transfer of the tissue culture bottle; the rough washing station flushing pipe is provided with a plurality of nozzles for flushing the tissue culture bottles on the first electric rotating frame; The fine washing station is provided with a second electric rotating frame, a fine washing station flushing pipe, a fine washing water tank arranged below the second electric rotating frame, and an output track; the second electric rotating frame is also provided with a plurality of washing tracks along the circumferential direction and can rotate and switch the washing tracks, each washing track is also provided with a limit frame, and the second electric rotating frame and the first electric rotating frame are docked through a transition track located on the frame to realize the transfer of tissue culture bottles; the fine washing station flushing pipe is also provided with a plurality of nozzles; the output track can be docked with the washing track to facilitate the output of tissue culture bottles after fine washing is completed; A water purifier, connected to the rough wash water tank through a sewage inlet pipeline, and connected to the water inlet of the circulation pump through a clean water output pipeline; A main water inlet pump is connected to a tap water source and is connected to the rough washing station flushing pipe and the fine washing station flushing pipe through a main water outlet pipe; A circulation pump, whose water inlet is connected to the water inlet of the fine washing water tank, and whose water outlet is connected to the flushing pipe of the rough washing station; Wherein, the rough wash water tank and the fine wash water tank are both provided with a water tank overflow alarm sensor and a water tank low level alarm sensor; The invention also includes a water replenishment method of the main water inlet pump, which specifically includes the following steps: Real-time data collection, including the start time t of each cleaning at the rough cleaning station 粗洗开始 and end time t 粗洗结束 , the starting time of each cleaning at the fine cleaning station t 精洗开始 and end time t 精洗结束 , ambient temperature Tm, water volume treated by the water purifier Q 已处理 , the real-time power P of the main water inlet pump and the circulation pump 主 and P 循 ; Set initial parameters, including rough wash time threshold T 粗洗阈值 , fine washing time threshold T 精洗阈值 , Ambient temperature threshold T 高 and T 低 、Rated water treatment volume of water purifier Q 额定 , energy consumption threshold E of main water inlet pump and circulation pump 主 and E 循 , time interval threshold T, rough wash water tank water level lower limit threshold L, water replenishment volume V 补 , rough washing time influence coefficient k1, fine washing time influence coefficient k2, temperature compensation coefficient k3, low temperature adjustment coefficient k4, remaining life warning coefficient k5, water purifier life influence adjustment value k7, main water inlet pump energy consumption adjustment coefficient k8, circulation pump energy consumption adjustment coefficient k9; Calculate the actual time T for each rough cleaning operation 粗实际 and the actual time T of the fine washing operation 精实际 ; When T 粗实际 >T 粗洗阈值 When the time interval threshold T is adjusted to T 1 =T-k1(T 粗实际 -T 粗洗阈值 ) to speed up the frequency of water replenishment; When T 精实际 >T 精洗阈值 When the water level in the fine washing station water tank is lower than the preset proportion of its full water level, the main water inlet pump is immediately started to partially replenish water, and the time interval threshold T is adjusted to T 2 =T-k2(T 精实际 -T 精洗阈值 ); When Tm>T 高 When the temperature compensation coefficient K is calculated T =k3(Tm-T 高 ), adjust the time interval threshold T to T 3 =T / (1+K T ), and increase the water replenishment V 补 =V 补 (1+K T ) to compensate for the water loss caused by high temperature evaporation; When Tm<T 低 When the water supply is sufficient, the operating frequency of the main water inlet pump and the circulation pump is reduced to reduce energy consumption, and the time interval threshold T is adjusted to T 4 =T(1+k4(T 低 -Tm)); Preset amount per treatment Q 监测 water, update the treated water volume Q 已处理 =Q 已处理 +Q 监测 ; When Q 已处理 >(1-k5)Q 额定 When the life span of the water purifier is over, a warning signal is issued, and after the warning, the proportion of circulating water usage is reduced, the flow rate of the circulating pump is reduced to (1-k6) times of the original, and the replenishment frequency of the main water inlet pump is increased, and the time interval threshold T is updated to T 5 =T-k7, to extend the life of the water purifier; Calculate the average energy consumption E of the main water inlet pump and circulation pump within a certain period of time 主平均 and E 循平均 ; When E 主平均 >E 主 When the power of the main water inlet pump is reduced, the time interval threshold T is updated to T 6 =T+k8, to reduce unnecessary water replenishment operations; When E 循平均 >E 循 When the water demand is high, reduce the flow rate of the circulation pump to (1-k9) times of the original value, and adjust its running time according to the actual water demand; After the operation is stable or the preset cycle time is reached, the final time interval threshold T is obtained. final .

2. A water circulation device for a tissue culture bottle filling line as claimed in claim 1, characterized in that: The number of the rough washing station flushing pipe is one, which is arranged on the top of the first electric rotating frame, and each nozzle is vertically downward and corresponds to a tissue culture bottle; The number of the fine washing station flushing pipes is more than two, and they are respectively arranged on the top and side of the second electric rotating frame.

3. A water circulation device for a tissue culture bottle filling line as claimed in claim 1 or 2, characterized in that: Take T 1 -T 6 The minimum value among them is taken as the final time interval threshold T final .

4. A water circulation device for a tissue culture bottle filling line as claimed in claim 3, characterized in that: The water replenishment method also includes: When the water tank overflow alarm sensors of the fine washing station and the rough washing station are triggered, the main water inlet pump is turned off, the water purifier and the circulation pump are started, and the circulating sewage time t is recorded at the same time; When one of the following conditions is met, turn on the main water inlet pump to replenish water: Circulating sewage time t reaches T final ; The water level of the rough wash water tank detects the lower limit threshold value L of the rough wash water tank water level; When replenishing water, the final replenishment water volume V 补 Perform water replenishment operation to ensure that the water level in the rough wash water tank returns to the appropriate level.

5. A water circulation device for a tissue culture bottle filling line as claimed in claim 3, characterized in that: The value of the time interval threshold T is based on water consumption estimation, empirical value or experimental value.

6. A water circulation control method for a tissue culture bottle filling line, used in a water circulation device for a tissue culture bottle filling line according to any one of claims 1 to 5, characterized in that: The following steps are involved: Obtaining a start signal of the bottle-moving motor, controlling the main water inlet pump according to a preset initial cleaning program, and supplying water to the rough washing station at a preset flow rate; wherein a cleaning track of the first electric rotating rack filled with tissue culture bottles is regarded as a batch; As the next batch of tissue culture bottles enter the first electric rotating rack, the first electric rotating rack is continuously rotated intermittently to perform a rough washing operation, while dynamically adjusting the power of the main water inlet pump and monitoring the water level of the rough washing water tank in real time; After the number of times or the cumulative flushing time of the first batch of tissue culture bottles reaches a preset threshold, the cleaning track where the first batch of tissue culture bottles are located is rotated to the highest position and docked with the cleaning track on the second electric rotating frame. After the docking is completed, the bottle shifting motor is turned on, and the feeding and transfer of the tissue culture bottles are performed at the same time, so that the tissue culture bottles to be cleaned enter the first electric rotating frame, and the tissue culture bottles after the rough washing are entered into the second electric rotating frame; The main water inlet pump is controlled according to the preset fine washing program to supply water to the rough washing station and the fine washing station at the preset flow rate at the same time; As the next batch of tissue culture bottles enter the second electric rotating rack, the second electric rotating rack is continuously rotated intermittently to perform fine washing operations, while dynamically adjusting the power of the main water inlet pump and monitoring the water level of the fine washing water tank in real time; After the number of times or the cumulative flushing time of the first batch of tissue culture bottles reaches a preset threshold, the cleaning track where the first batch of tissue culture bottles are located is rotated to the highest position, and docked with the cleaning track and the output track on the first electric rotating frame. After docking, the bottle-moving motor is turned on, and the feeding, transfer and output of the tissue culture bottles are performed at the same time, so that the tissue culture bottles to be cleaned enter the first electric rotating frame, the tissue culture bottles after rough washing enter the second electric rotating frame, and the tissue culture bottles after fine washing are output to the output track; When the water tank low level alarm sensors of both the fine washing station and the rough washing station are triggered, the circulation pump is turned off; When the water tank overflow alarm sensor of the fine wash station is triggered, the circulation pump is turned on to draw water from the fine wash water tank to supply the rough wash spray pipe; When the water tank overflow alarm sensor of the rough wash station is triggered, the water purifier is turned on to extract water from the rough wash water tank for purification and then discharged to the circulation pump, and the circulation pump is turned on to supply water to the rough wash spray pipe; When the water tank low level alarm sensor at the rough wash station is triggered, the water purifier is turned off; When the water tank overflow alarm sensors of the fine washing station and the rough washing station are triggered, the main water inlet pump is turned off and the water purifier and circulation pump are started.

7. A water circulation control method for a tissue culture bottle filling line as claimed in claim 6, characterized in that: It also includes a water purifier life monitoring model, which is used to predict the remaining life of the water purifier. The method for establishing the water purifier life monitoring model records the working hours of the water purifier, uses a flow sensor to monitor the cumulative treated water volume, and uses a variety of water quality sensors to monitor water quality changes. The life prediction models are constructed and weights are assigned respectively, and a comprehensive model is constructed to evaluate the remaining life in real time, and a warning signal is issued according to the remaining life warning value and the model parameters are continuously optimized.

8. An electronic device comprising a memory and a processor, characterized in that: A computer program is stored in the memory, and the processor is configured to run the computer program to execute the water circulation control method for the tissue culture bottle filling line according to claim 6 or 7.

9. A readable storage medium, characterized in that: A computer program is stored in the readable storage medium, and the computer program includes a program code for controlling a process to execute a process, and the process includes the water circulation control method for a tissue culture bottle filling line according to claim 6 or 7.

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