A kitchen oil stain detergent and its preparation method

Abstract

The present invention provides a kitchen oil stain detergent and a preparation method thereof, belonging to the technical field of detergents. A porous hollow microsphere with lipophilic interior and hydrophilic exterior is prepared, and after adsorbing alkaline lipase and alkaline protease, it is mixed with the fermentation products obtained by fermenting Chinese honey locust, orange peel and lemon peel, and the broken wall products obtained by breaking the wall of fermented bacteria. Isopropanol, water and essence are added and mixed evenly to prepare the kitchen oil stain detergent. The kitchen oil stain detergent prepared by the present invention has a good removal effect on stubborn kitchen stains by adopting the method of composite treatment of organic solvent + surfactant + enzyme + physical adsorption, with fast cleaning speed, good effect, safety and environmental protection, mildness, low toxicity, small irritation, low cost and good stability, and has broad application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of detergents, and specifically relates to a kitchen oil stain detergent and a preparation method thereof. Background Art

[0002] The formation of kitchen dirt is mainly due to the dripping of cooking oil and partial evaporation by heat, which contaminates utensils. Coupled with dust, a viscous and highly adhesive oil stain is formed over time. Such oil stains are difficult to remove with ordinary detergents. At present, the common kitchen oil stain cleaning mainly adopts strong alkaline cleaning and solvent-based cleaning technologies. Although both have a high oil stain removal rate, their use safety is poor, and they have a great corrosive and harmful effect on human skin and kitchen facilities. At the same time, if the wastewater of these detergents is discharged into rivers through the sewer after home cleaning, it directly pollutes the river water. When introduced into aquaculture or planting, the surfactants in it are absorbed by these animals and plants. People who eat these contaminated animals and plants for a long time will cause great harm to their bodies.

[0003] Therefore, the development of a highly efficient, safe and environmentally friendly kitchen oil stain detergent has great application value and development prospects. Summary of the Invention

[0004] The purpose of the present invention is to provide a kitchen oil stain detergent and a preparation method thereof. By adopting the method of compound treatment of organic solvent + surfactant + enzyme + physical adsorption, it has a good removal effect on stubborn kitchen stains, with a fast cleaning speed, good effect, safety and environmental protection, mildness, low toxicity, small irritation, low cost, good stability, and broad application prospects.

[0005] The technical solution of the present invention is realized as follows:

[0006] The present invention provides a preparation method of a kitchen oil stain detergent, which prepares a porous hollow microsphere with lipophilic interior and hydrophilic exterior, adsorbs alkaline lipase and alkaline protease, and then mixes with the fermentation product obtained after fermenting with Chinese honey locust, orange peel and lemon peel, and the broken wall product obtained by breaking the wall of fermented bacteria, and adds isopropanol, water and essence and mixes evenly to obtain the kitchen oil stain detergent.

[0007] As a further improvement of the present invention, it includes the following steps:

[0008] S1. Preparation of porous hollow microsphere with lipophilic interior and hydrophilic exterior: Drop aminopropylsilane, long-chain alkylsilane and pore-forming agent into an aqueous solution containing emulsifier, emulsify, stir and react, and spray dry to obtain a porous hollow microsphere with lipophilic interior and hydrophilic exterior;

[0009] S2. Adsorption of compound enzyme: alkaline lipase and alkaline protease were mixed evenly, added to water, and porous hollow microspheres with lipophilic inner and hydrophilic outer were added, stirred for reaction, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres;

[0010] S3. Fermentation: Gleditsia sinensis, orange peel and lemon peel were mixed and crushed, added to water, inoculated with fermentation bacteria, fermented and cultured, filtered, the solid solution was washed and collected, freeze-dried, and a composite bacteria was obtained; the filtrate was dried to obtain a fermentation product;

[0011] S4. Breaking the wall of the bacterial sludge: adding the composite bacteria to water, adding snail enzyme and lysozyme, heating and stirring for enzymolysis, filtering, and drying the filtrate to obtain a broken wall product;

[0012] S5. Preparation of kitchen grease detergent: Evenly mix the immobilized enzyme degreasing microspheres, fermentation products, broken cell products, isopropanol, water and essence, adjust the pH value of the solution, and prepare the kitchen grease detergent.

[0013] As a further improvement of the present invention, the mass ratio of the aminosilane, the long-chain alkyl silane, the porogen and the emulsifier in step S1 is 12-15:10-12:1-2:0.5-1:0.5-1, the aminosilane is selected from at least one of KH550, KH602 and KH792, the long-chain alkyl silane is selected from at least one of dodecyltrimethoxysilane, dodecyltriethoxysilane, dodecylmethyldimethoxysilane, hexadecyltrimethoxysilane and octadecyltrimethoxysilane, the porogen is selected from at least one of hexadecyltrimethylammonium chloride, hexadecyldimethylbenzylammonium chloride and hexadecyltrimethylammonium bromide, the emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85, and the stirring reaction time is 5-7h.

[0014] As a further improvement of the present invention, in step S2, the mass ratio of alkaline lipase, alkaline protease and porous hollow microspheres with inner lipophilicity and outer hydrophilicity is 3-4:1-2:12-16, and the stirring reaction time is 2-3h.

[0015] As a further improvement of the present invention, the mass ratio of the sapodilla, orange peel and lemon peel in step S3 is 7-10:4-6:2-4, the fermentation bacteria are Bacillus licheniformis and Bifidobacterium lactis, and the inoculated bacteria are their seed liquids, and the bacterial content of the seed liquid is 10 8 -10 9 cfu / mL, the inoculation amounts were 2-3v / v% and 1-2v / v%, respectively, and the fermentation culture conditions were 39-41°C, 100-200r / min, and the fermentation culture was 36-48h.

[0016] As a further improvement of the present invention, in step S4, the mass ratio of the composite bacteria, snailase and lysozyme is 100:2-3:3-5, the temperature of the heating and stirring enzymolysis is 35-40 °C, and the time is 1-3 h.

[0017] As a further improvement of the present invention, in step S5, the mass ratio of the immobilized enzyme deoiling microspheres, fermentation product, cell wall broken product, ethanol, isopropanol, water and essence is 10-15:4-6:3-5:15-25:7-12:100-150:0.5-1, and the pH value of the adjusted solution is 7.3-7.5.

[0018] As a further improvement of the present invention, it specifically includes the following steps:

[0019] S1. Preparation of porous hollow inner lipophilic and outer hydrophilic microspheres: Drop 12-15 parts by weight of amino silane, 10-12 parts by weight of long-chain alkyl silane, and 1-2 parts by weight of pore-forming agent into an aqueous solution containing 0.5-1 part by weight of emulsifier, emulsify, stir and react for 5-7 h, and spray dry to obtain porous hollow inner lipophilic and outer hydrophilic microspheres;

[0020] S2. Adsorption of composite enzyme: Mix 3-4 parts by weight of alkaline lipase and 1-2 parts by weight of alkaline protease evenly, add to water, add 12-16 parts by weight of porous hollow inner lipophilic and outer hydrophilic microspheres, stir and react for 2-3 h, centrifuge, wash, and dry to obtain immobilized enzyme deoiling microspheres;

[0021] S3. Fermentation: Mix and crush 7-10 parts by weight of Chinese honey locust, 4-6 parts by weight of orange peel, and 2-4 parts by weight of lemon peel, add to 200 parts by weight of water, inoculate the seed solutions of Bacillus licheniformis and Bifidobacterium lactis, and the inoculation amounts are 2-3 v / v% and 1-2 v / v% respectively, ferment and culture at 39-41 °C and 100-200 r / min for 36-48 h, filter, wash the solid to collect the bacterial liquid, and freeze-dry to obtain composite bacteria; dry the filtrate to obtain the fermentation product;

[0022] S4. Cell wall breaking of bacterial sludge: Add 10 parts by weight of composite bacteria to water, add 0.2-0.3 parts by weight of snailase and 0.3-0.5 parts by weight of lysozyme, heat to 35-40 °C, stir and enzymolyze for 1-3 h, filter, and dry the filtrate to obtain the cell wall broken product;

[0023] S5. Preparation of kitchen oil stain detergent: Mix 10-15 parts by weight of immobilized enzyme deoiling microspheres, 4-6 parts by weight of fermentation product, 3-5 parts by weight of cell wall broken product, 7-12 parts by weight of isopropanol, 100-150 parts by weight of water and 0.5-1 part by weight of essence evenly, adjust the pH value of the solution to 7.3-7.5, and obtain the kitchen oil stain detergent.

[0024] The present invention further protects a kitchen oil stain detergent prepared by the above-mentioned preparation method.

[0025] The present invention further protects the application of the above-mentioned kitchen oil stain detergent in cleaning oil stains and dirt.

[0026] The present invention has the following beneficial effects:

[0027] Kitchen oil stains are generally cross-linked by polymers of oils and fats and other dirt, which are difficult to dissolve in water and are also difficult to be emulsified and solubilized under conventional conditions. Therefore, it increases the difficulty of cleaning kitchen oil stains. The present invention adopts a method of composite treatment of organic solvent + surfactant + enzyme + physical adsorption. Among them, an organic solvent such as isopropanol can effectively clean the polymers in the oil stains through wetting, penetration, dissolution and emulsification. However, a large amount of organic solvents are prone to problems such as strong irritation and high storage risk. Therefore, the present invention adds a small amount of isopropanol. The present invention does not directly add surfactants, but through the fermentation of Chinese honey locust, orange peel and lemon peel by fermentation bacteria (Bacillus licheniformis and Bifidobacterium lactis), a large amount of surface active substances can be produced, which have the characteristics of green, high yield, environmental protection, safety, etc. Chinese honey locust, orange peel and lemon peel itself contain rich saponins, sapindol, saponins, sapinol, hesperidin, naringin, alkaloids, essential oils, etc. After fermentation, rich surfactant substances are produced, and it also has good antioxidant, fragrance and other effects, improving the stability of the prepared kitchen oil stain detergent;

[0028] In addition, through the synergistic cell wall breaking effect of snailase and lysozyme on the fermentation bacteria themselves, the cell walls of the fermentation bacteria can be damaged, so that the content is greatly dissolved, greatly increasing the content of surface active substances, and thus significantly improving the decontamination rate and oil removal rate. The surface active substances can make the oil stains fall off and separate from the object surface through wetting, penetration, solubilization and emulsification and other effects, and have the characteristics of mildness, low toxicity and small irritation. At the same time, compared with directly adding surfactants, the surface active substances produced by microorganisms in the present invention have the advantages of low cost, good effect, reduction of environmental pollution, safety and environmental protection, etc.

[0029] The present invention prepares porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces. Amino silane and long-chain alkyl silane cannot be dissolved in water initially, but are dispersed into small droplets during the stirring and emulsification process. The amino group will be protonated to become amphiphilic molecules, further stabilizing the silane droplets and providing an alkaline environment to catalyze the sol-gel reaction process of the silane. The consumption of the inner silane will result in a hollow sphere structure. Due to the hydrophobicity of the long-chain alkyl, it will spontaneously face the inside of the shell, thus forming a silica hollow sphere with amino groups on the outer surface and long-chain alkyl groups on the inner surface. Under the action of the pore-forming agent, the silica shell layer presents a porous structure, increasing its specific surface area and providing a rich site for subsequent adsorption and immobilization of enzymes. At the same time, due to the rich hydrophobic long-chain alkyl groups in the inner layer of the hollow sphere, it can adsorb oil stains into the interior of the hollow sphere and be enzymatically hydrolyzed by the composite enzyme loaded on it, improving the efficiency of removing oil stains.

[0030] The composite enzyme of the present invention includes alkaline lipase and alkaline protease, which can improve the decontamination ability of kitchen oil stains, reduce the usage of additives and surfactants in detergents, and reduce environmental pollution. Kitchen oil stains are mainly formed by the accumulation of splashed vegetable oil used daily. The main component of vegetable oil is triglyceride. Alkaline lipase can hydrolyze triglyceride into fatty acid and glycerol to achieve the effect of cleaning kitchen oil stains. At the same time, some proteinaceous mixed stains can also be enzymatically hydrolyzed together, thus playing a synergistic effect.

[0031] The kitchen oil stain detergent prepared by the present invention has a good removal effect on stubborn kitchen stains by adopting the method of composite treatment of organic solvent + surfactant + enzyme + physical adsorption, with a fast cleaning speed, good effect, being safe and environmentally friendly, mild, low in toxicity and irritation, low in cost, and good in stability, and has broad application prospects. Detailed implementation method

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0033] Bacillus licheniformis, 20 billion cfu / g, purchased from Guangzhou Weiyuan Biotechnology Co., Ltd. Bifidobacterium lactis, 10 billion cfu / g, Qingdao North Biotechnology Co., Ltd.

[0034] Preparation method of the strain seed solution: Inoculate the strain into the Czapek medium, and at the optimal temperature, culture it at 150 r / min for 18 - 24 h to obtain a strain seed solution with a bacteria content of 10 8 -10 9 cfu / mL.

[0035] Snail enzyme, with a cell wall breaking rate of 90%, was purchased from Shanghai Yuanye Bio-Technology Co., Ltd.; Lysozyme, 20,000 U / g, was purchased from Nanning Dongheng Huadao Bio-Technology Co., Ltd.

[0036] Alkaline lipase, 100,000 U / g, and alkaline protease, 50,000 U / g, were purchased from Wuxi Youpuke Bio-Technology Co., Ltd.

[0037] Example 1

[0038] This example provides a preparation method of a kitchen oil stain detergent, which specifically includes the following steps:

[0039] S1. Preparation of porous hollow microspheres with lipophilic interior and hydrophilic exterior: 12 g of silane coupling agent KH550, 10 g of dodecyltrimethoxysilane, and 1 g of cetyltrimethylammonium chloride were dropped into 200 mL of an aqueous solution containing 0.5 g of Tween-20, emulsified at 5000 r / min for 15 min, stirred and reacted for 5 h, and spray-dried to obtain porous hollow microspheres with lipophilic interior and hydrophilic exterior;

[0040] S2. Adsorption of composite enzyme: 3 g of alkaline lipase and 1 g of alkaline protease were mixed evenly, added to 100 mL of water, 12 g of porous hollow microspheres with lipophilic interior and hydrophilic exterior were added, stirred and reacted for 2 h, centrifuged, washed, and dried to obtain immobilized enzyme oil-removing microspheres;

[0041] S3. Fermentation: 7 g of Chinese honey locust, 4 g of orange peel, and 2 g of lemon peel were mixed and pulverized, added to 200 mL of water, inoculated with the seed solutions of Bacillus licheniformis and Bifidobacterium lactis, with the inoculation amounts being 2 v / v% and 1 v / v% respectively, fermented and cultured at 39 °C and 100 r / min for 36 h, filtered, the solid was washed and the bacterial liquid was collected, and freeze-dried to obtain a composite bacterium; the filtrate was dried to obtain a fermentation product;

[0042] S4. Cell wall breaking of the bacterial sludge: 10 g of the composite bacterium was added to 100 mL of water, 0.2 g of snail enzyme and 0.3 g of lysozyme were added, heated to 35 °C, stirred and enzymolyzed for 1 h, filtered, and the filtrate was dried to obtain a cell wall-breaking product;

[0043] S5. Preparation of the kitchen oil stain detergent: 10 g of the immobilized enzyme oil-removing microspheres, 4 g of the fermentation product, 3 g of the cell wall-breaking product, 7 g of isopropanol, 100 mL of water, and 0.5 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.3 to obtain the kitchen oil stain detergent.

[0044] Example 2

[0045] This example provides a preparation method of a kitchen oil stain detergent, which specifically includes the following steps:

[0046] S1. Preparation of porous hollow microspheres with lipophilic interior and hydrophilic exterior: Drop 15 g of silane coupling agent KH602, 12 g of dodecylmethyldimethoxysilane, and 2 g of cetyl dimethyl benzyl ammonium chloride into 200 mL of an aqueous solution containing 1 g of Tween-40, emulsify at 5000 r / min for 15 min, stir and react for 7 h, and spray dry to obtain porous hollow microspheres with lipophilic interior and hydrophilic exterior;

[0047] S2. Adsorption of composite enzyme: Mix 4 g of alkaline lipase and 2 g of alkaline protease evenly, add them to 100 mL of water, add 16 g of porous hollow microspheres with lipophilic interior and hydrophilic exterior, stir and react for 3 h, centrifuge, wash, and dry to obtain immobilized enzyme oil-removing microspheres;

[0048] S3. Fermentation: Mix 10 g of Chinese honey locust, 6 g of orange peel, and 4 g of lemon peel, crush them, add them to 200 mL of water, inoculate with the seed solutions of Bacillus licheniformis and Bifidobacterium lactis, with the inoculation amounts being 3 v / v% and 2 v / v% respectively, ferment and culture at 41 °C and 200 r / min for 48 h, filter, wash the solid to collect the bacterial liquid, and freeze-dry to obtain the composite bacteria; dry the filtrate to obtain the fermentation product;

[0049] S4. Cell wall breaking of bacterial sludge: Add 10 g of the composite bacteria to 100 mL of water, add 0.3 g of snail enzyme and 0.5 g of lysozyme, heat to 40 °C, stir and enzymatically hydrolyze for 3 h, filter, and dry the filtrate to obtain the cell wall-breaking product;

[0050] S5. Preparation of kitchen oil stain detergent: Mix 15 g of immobilized enzyme oil-removing microspheres, 6 g of fermentation product, 5 g of cell wall-breaking product, 12 g of isopropanol, 150 mL of water, and 1 g of bergamot essence, stir and mix for 15 min, and adjust the pH value of the solution to 7.5 to obtain the kitchen oil stain detergent.

[0051] Example 3

[0052] This example provides a method for preparing a kitchen oil stain detergent, which specifically includes the following steps:

[0053] S1. Preparation of porous hollow microspheres with lipophilic interior and hydrophilic exterior: Drop 13.5 g of silane coupling agent KH792, 11 g of octadecyltrimethoxysilane, and 1.5 g of cetyltrimethylammonium bromide into 200 mL of an aqueous solution containing 0.7 g of Tween-80, emulsify at 5000 r / min for 15 min, stir and react for 6 h, and spray dry to obtain porous hollow microspheres with lipophilic interior and hydrophilic exterior;

[0054] S2. Adsorption of composite enzyme: Mix 3.5 g of alkaline lipase and 1.5 g of alkaline protease evenly, add them to 100 mL of water, add 14 g of porous hollow microspheres with lipophilic interior and hydrophilic exterior, stir and react for 2.5 h, centrifuge, wash, and dry to obtain immobilized enzyme oil-removing microspheres;

[0055] S3. Fermentation: 8 g of sapodilla, 5 g of orange peel, 3 g of lemon peel were mixed and crushed, added to 200 mL of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amounts were 2.5 v / v% and 1.5 v / v%, 40 ° C, 150 r / min, fermentation culture 42h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product;

[0056] S4. Breaking the bacterial sludge: 10 g of the composite bacteria was added to 100 mL of water, 0.25 g of snail enzyme and 0.4 g of lysozyme were added, heated to 37 ° C, stirred for enzymolysis for 2 h, filtered, and the filtrate was dried to obtain a broken product;

[0057] S5. Preparation of kitchen grease detergent: 12 g of immobilized enzyme degreasing microspheres, 5 g of fermentation product, 4 g of broken cell wall product, 10 g of isopropanol, 125 mL of water and 0.7 g of bergamot essence were stirred and mixed for 15 min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0058] Comparative Example 1

[0059] Compared with Example 3, the difference is that the porogen hexadecyltrimethylammonium bromide is not added in step S1.

[0060] The details are as follows:

[0061] S1. Preparation of hollow microspheres with lipophilic inner part and hydrophilic outer part: 13.5 g of silane coupling agent KH792 and 11 g of octadecyltrimethoxysilane were added into 200 mL of aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for reaction for 6 h, and spray dried to obtain hollow microspheres with lipophilic inner part and hydrophilic outer part.

[0062] Comparative Example 2

[0063] Compared with Example 3, the difference is that octadecyltrimethoxysilane is not added in step S1.

[0064] The details are as follows:

[0065] S1. Preparation of porous hollow microspheres: 24.5 g of silane coupling agent KH792 and 1.5 g of hexadecyltrimethylammonium bromide were added to 200 mL of an aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for reaction for 6 h, and spray-dried to obtain porous hollow microspheres.

[0066] Comparative Example 3

[0067] Compared with Example 3, the difference is that alkaline lipase is not added in step S2.

[0068] The details are as follows:

[0069] S2. Adsorption of composite enzyme: 5 g alkaline protease was mixed evenly, added into 100 mL water, and 14 g porous hollow microspheres with lipophilic inner part and hydrophilic outer part were added, stirred for reaction for 2.5 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres.

[0070] Comparative Example 4

[0071] Compared with Example 3, the difference is that alkaline protease is not added in step S2.

[0072] The details are as follows:

[0073] S2. Adsorption of composite enzyme: 5 g alkaline lipase was mixed evenly, added into 100 mL water, and 14 g porous hollow microspheres with lipophilic inner part and hydrophilic outer part were added, stirred for reaction for 2.5 h, centrifuged, washed, and dried to obtain immobilized enzyme degreasing microspheres.

[0074] Comparative Example 5

[0075] Compared with Example 3, the difference is that step S2 is not performed.

[0076] The details are as follows:

[0077] S1. Preparation of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces: 13.5 g of silane coupling agent KH792, 11 g of octadecyltrimethoxysilane, and 1.5 g of hexadecyltrimethylammonium bromide were added to 200 mL of an aqueous solution containing 0.7 g of Tween-80, emulsified at 5000 r / min for 15 min, stirred for 6 h, and spray-dried to obtain porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces;

[0078] S2. Fermentation: 8 g of sapodilla, 5 g of orange peel, 3 g of lemon peel were mixed and crushed, added to 200 mL of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amounts were 2.5 v / v% and 1.5 v / v%, 40 ° C, 150 r / min, fermentation culture 42h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product;

[0079] S3. Breaking the bacterial sludge: 10 g of the composite bacteria was added to 100 mL of water, 0.25 g of snail enzyme and 0.4 g of lysozyme were added, heated to 37 ° C, stirred for enzymolysis for 2 h, filtered, and the filtrate was dried to obtain a broken product;

[0080] S4. Preparation of kitchen grease detergent: 12g of porous hollow microspheres with lipophilic inner and hydrophilic outer surfaces, 5g of fermentation product, 4g of broken wall product, 10g of isopropanol, 125mL of water and 0.7g of bergamot essence were stirred and mixed for 15min, and the pH value of the solution was adjusted to 7.4 to prepare a kitchen grease detergent.

[0081] Comparative Example 6

[0082] Compared with Example 3, the difference lies in that the Bacillus licheniformis strain seed liquid was not inoculated in step S3.

[0083] Specifically as follows:

[0084] S3. Fermentation: Mix 8 g of Chinese honey locust, 5 g of orange peel, and 3 g of lemon peel and crush them, add 200 mL of water, inoculate with the Bifidobacterium lactis strain seed liquid, the inoculation amount is 4 v / v%, at 40 °C, 150 r / min, ferment and culture for 42 h, filter, wash the solid to collect the bacterial liquid, and freeze-dry to obtain the composite bacteria; dry the filtrate to obtain the fermentation product.

[0085] Comparative Example 7

[0086] Compared with Example 3, the difference lies in that the Bifidobacterium lactis strain seed liquid was not inoculated in step S3.

[0087] Specifically as follows:

[0088] S3. Fermentation: Mix 8 g of Chinese honey locust, 5 g of orange peel, and 3 g of lemon peel and crush them, add 200 mL of water, inoculate with the Bacillus licheniformis strain seed liquid, the inoculation amount is 4 v / v%, at 40 °C, 150 r / min, ferment and culture for 42 h, filter, wash the solid to collect the bacterial liquid, and freeze-dry to obtain the composite bacteria; dry the filtrate to obtain the fermentation product.

[0089] Comparative Example 8

[0090] Compared with Example 3, the difference lies in that the immobilized enzyme deoiling microspheres were not added in step S5.

[0091] Specifically as follows:

[0092] S5. Preparation of kitchen oil stain detergent: Mix 5 g of fermentation product, 4 g of broken wall product, 10 g of isopropanol, 125 mL of water, and 0.7 g of bergamot essence, stir and mix for 15 min, and adjust the pH value of the solution to 7.4 to obtain the kitchen oil stain detergent.

[0093] Comparative Example 9

[0094] Compared with Example 3, the difference lies in that the fermentation product was not added in step S5.

[0095] Specifically as follows:

[0096] S5. Preparation of kitchen oil stain detergent: Mix 12 g of immobilized enzyme deoiling microspheres, 4 g of broken wall product, 10 g of isopropanol, 125 mL of water, and 0.7 g of bergamot essence, stir and mix for 15 min, and adjust the pH value of the solution to 7.4 to obtain the kitchen oil stain detergent.

[0097] Comparative Example 10

[0098] Compared with Example 3, the difference lies in that the broken wall product was not added in step S5.

[0099] Specifically as follows:

[0100] S5. Preparation of kitchen oil stain detergent: Mix 12 g of immobilized enzyme degreasing microspheres, 5 g of fermentation product, 10 g of isopropanol, 125 mL of water, and 0.7 g of bergamot essence, stir and mix for 15 min, and adjust the pH value of the solution to 7.4 to obtain the kitchen oil stain detergent.

[0101] Test Example 1 Determination of Biosurfactant Yield

[0102] Adjust the pH value of the fermentation products and broken wall products prepared in Examples 1-3 and Comparative Examples 6 and 7 of the present invention to 2.0 with 6 mol / L HCl, extract twice with an equal volume of a chloroform:methanol mixed solvent with a volume ratio of 2:1, collect the organic phase, and dry to obtain the surface active substance, and calculate the yield. The results are shown in Table 1.

[0103] Yield = mass of surface active substance / mass of fermentation product or broken wall product × 100%

[0104] Table 1

[0105]

[0106] As can be seen from the above table, the fermentation products and broken wall products prepared in Examples 1-3 of the present invention contain a relatively high content of surface active substances.

[0107] Test Example 2 Determination of Oil Drainage Performance

[0108] Take a petri dish with a diameter of 20 cm, add 50 mL of water, add 2 mL of rapeseed oil in the center, wait for the oil film to spread evenly, and then add 1 drop of the kitchen oil stain detergent prepared in Examples 1-3 and Comparative Examples 1-10 in the center, and measure the diameter of the oil drainage circle. The results are shown in Table 2.

[0109] Table 2

[0110]

[0111]

[0112] As can be seen from the above table, the kitchen oil stain detergents prepared in Examples 1-3 of the present invention have good oil drainage performance.

[0113] Test Example 3

[0114] Test the decontamination and degreasing effects of the kitchen oil stain detergents prepared in Examples 1-3 and Comparative Examples 1-10 of the present invention, and the results are shown in Table 3.

[0115] Test of detergency rate:

[0116] Scrub the glassware clean with anhydrous ethanol, dry it and weigh it, record as M1. Pour the stain (a mixture of vegetable oil and lard in equal amounts accounting for 15%, wheat flour accounting for 15%, whole milk powder accounting for 7%, egg liquid accounting for 30%, distilled water accounting for 33%) into a plate, soak for 10 s, dry for 30 min, scrub the oil that has not been absorbed on the plate and weigh it again, record as M2. Dilute the kitchen oil stain detergent 100 times, control the water temperature at (35±2)°C, put the plate into it, stir it twice with a stick, then dry and cool the plate, and weigh it again, record as M3. The calculation formula is as follows:

[0117] Detergency rate (%) = (M2 - M3) / (M2 - M1)×100%

[0118] Test of oil removal rate:

[0119] Scrub the glassware clean with anhydrous ethanol, dry it and weigh it, record as M1. Pour vegetable oil into the tableware, soak for 10 s, dry in the sun for 30 min, wipe off the oil that has not been absorbed on the tableware with filter paper and weigh it again, record as M2. Dilute the kitchen oil stain detergent 100 times. Add 2500 mL to each 5 L plastic bucket, control the water temperature at (35±2)°C, soak the tableware with oil stains in the detergent solution, shake or stir it slightly for 30 s, then dry and cool the tableware, and weigh it, record as M3, and calculate the oil removal rate.

[0120] Oil removal rate (%) = (M2 - M3) / (M2 - M1)×100%

[0121] Table 3

[0122] Group Detergency rate (%) Degreasing rate (%) Example 1 97.8 98.9 Example 2 97.4 99.2 Example 3 98.0 99.5 Comparative Example 1 93.9 94.1 Comparative Example 2 95.6 92.8 Comparative Example 3 94.9 90.7 Comparative Example 4 90.8 93.9 Comparative Example 5 88.7 87.2 Comparative Example 6 91.1 92.4 Comparative Example 7 90.7 91.1 Comparative Example 8 84.1 81.7 Comparative Example 9 86.9 87.2 Comparative Example 10 85.5 84.9

[0123] As can be seen from the above table, the kitchen oil stain detergents prepared in Examples 1 - 3 of the present invention have good detergency and oil removal performance.

[0124] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A preparation method of a kitchen oil stain detergent, characterized in that, The specific steps include: S1. Preparation of porous hollow lipophilic and hydrophilic microspheres: 12-15 parts by weight of aminosilane, 10-12 parts by weight of long-chain alkyl silane, and 1-2 parts by weight of porogen are dropped into an aqueous solution containing 0.5-1 parts by weight of an emulsifier, emulsified, stirred for 5-7h, and spray-dried to obtain porous hollow lipophilic and hydrophilic microspheres; S2. Adsorption of complex enzyme: 3-4 parts by weight of alkaline lipase and 1-2 parts by weight of alkaline protease were mixed and added to water, 12-16 parts by weight of porous hollow lipophilic and hydrophilic microspheres were added, the reaction was stirred for 2-3h, centrifuged, washed and dried to obtain immobilized enzyme degreasing microspheres; S3. Fermentation: 7-10 parts by weight of sapodilla, 4-6 parts by weight of orange peel, 2-4 parts by weight of lemon peel were mixed and crushed, added to 200 parts by weight of water, inoculated with Bacillus licheniformis and Bifidobacterium lactis seed solution, the inoculation amount was 2-3v / v% and 1-2v / v%, 39-41 ° C, 100-200r / min, fermentation culture 36-48h, filtered, solid washing and collecting the bacterial solution, freeze-dried to obtain a composite bacteria; the filtrate was dried to obtain a fermentation product; S4. Broken wall of bacterial sludge: 10 parts by weight of composite bacteria were added to water, 0.2-0.3 parts by weight of snail enzyme and 0.3-0.5 parts by weight of lysozyme were added, heated to 35-40 ° C, stirred for enzymatic hydrolysis for 1-3h, filtered, and the filtrate was dried to obtain a broken wall product; S5. Preparation of kitchen grease detergent: 10-15 parts by weight of immobilized enzyme degreasing microspheres, 4-6 parts by weight of fermentation products, 3-5 parts by weight of broken cell products, 7-12 parts by weight of isopropanol, 100-150 parts by weight of water and 0.5-1 parts by weight of flavor are mixed evenly, and the pH value of the solution is adjusted to 7.3-7.5 to prepare a kitchen grease detergent.

2. The preparation method according to claim 1, characterized in that, In step S1, the aminosilane is selected from at least one of KH550, KH602, and KH792, the long-chain alkylsilane is selected from at least one of dodecyltrimethoxysilane, dodecyltriethoxysilane, dodecylmethyldimethoxysilane, hexadecyltrimethoxysilane, and octadecyltrimethoxysilane, the porogen is selected from at least one of hexadecyltrimethylammonium chloride, hexadecyldimethylbenzylammonium chloride, and hexadecyltrimethylammonium bromide, and the emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80, and Tween-85.

3. A kitchen grease detergent prepared by the preparation method according to claim 1 or 2.

4. Use of the kitchen oil stain detergent as claimed in claim 3 in cleaning oil stains and stains.

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