Multi-channel tissue sampler and sampling method thereof

Abstract

The present invention relates to the technical field of tissue sampling, and provides a multi-channel tissue sampler and a sampling method thereof, wherein the multi-channel tissue sampler comprises a grip, a push rod, an elastic member, a pressing assembly and a sampling barrel; the pressing assembly comprises a pressing member and a rotating member, the pressing member, the rotating member and the pushing rod are sequentially installed in the grip, one end of the pressing member away from the rotating member is exposed to the grip, the pushing rod is movably arranged in the grip through the elastic member, a plurality of sampling barrels can be detachably installed in the grip, the sampling port of each sampling barrel is exposed to the grip, and the inner wall of the grip is provided with a limiting part matched with the pressing member and the rotating member; in the sampling state, under the action of the elastic member, the pressing member and the rotating member are aligned and arranged, and are both located at the upper limit of the limiting part in the axial direction of the grip; in the extrusion state, the pressing member and the rotating member are staggered, the rotating member abuts against the limiting part, and the pushing rod extends into each sampling barrel. In this way, intensive sampling can be completed in a short time.

Description

Technical Field

[0001] The invention relates to the technical field of tissue sampling, and in particular to a multi-channel tissue sampler and a sampling method thereof. Background Art

[0002] With the development of technology, transcriptome and proteome sequencing has gradually become an important means to analyze the structure and function of the primate brain. Recently, macaque brain proteome and transcriptome data have been released one after another, but most of these data are sparsely sampled and cannot widely cover the entire cerebral cortex. Tissue sampling before sequencing is a very important link, and the density of sampling determines the spatial resolution of the data. Commonly used tissue sampling tools are single-hole and can only obtain one sample at a time. Traditional tissue sampling tools make it difficult to complete dense sampling of the entire cerebral cortex of macaques in a short period of time. Summary of the invention

[0003] The invention provides a multi-channel tissue sampler and a sampling method thereof, which are used to solve the problem that the tissue sampling tools in the prior art are difficult to complete the intensive sampling of the whole cerebral cortex of macaques in a short time.

[0004] The present invention provides a multi-channel tissue sampler, comprising: a gripping rod, a pushing rod, an elastic member, a pressing assembly and a plurality of sampling barrels;

[0005] The pressing assembly comprises a pressing member and a rotating member, wherein the pressing member, the rotating member and the push rod are sequentially installed in the gripping rod, one end of the pressing member away from the rotating member is exposed to the gripping rod, the push rod is movably arranged in the gripping rod through the elastic member, a plurality of sampling barrels can be detachably installed in the gripping rod, and a sampling port of each sampling barrel is exposed to the gripping rod, and a limiting portion cooperating with the pressing member and the rotating member is arranged on the inner wall of the gripping rod;

[0006] Wherein, the multi-channel tissue sampler has a sampling state and an extrusion state. In the sampling state, under the action of the elastic member, the pressing member and the rotating member are aligned and both are located at the upper limit of the axial direction of the grip rod at the limit portion; in the extrusion state, the pressing member and the rotating member are staggered, and the rotating member abuts against the limit portion, and the push rod extends into each of the sampling tubes.

[0007] According to a multi-channel tissue sampler provided by the present invention, the pressing member comprises a barrel, a tooth portion and a first guide portion, the barrel is provided with the tooth portion at one end facing the rotating member, and a plurality of first guide portions are provided at intervals on the outer side surface of the barrel along the circumferential direction thereof;

[0008] The rotating member comprises a connecting portion and a plurality of second guide portions arranged in a circumference, two adjacent second guide portions are connected by the connecting portion, and one end of each second guide portion facing the tooth portion is inclined;

[0009] The limiting portion includes an annular body, a guide groove and an oblique groove. The guide groove and the oblique groove are both arranged on the annular body. A plurality of the guide grooves are arranged at intervals along the circumferential direction of the annular body. The oblique groove is located between two adjacent guide grooves and is connected to the notch of one of the guide grooves.

[0010] According to a multi-channel tissue sampler provided by the present invention, the rotating member further includes a rotating portion, and the rotating portion is connected to the connecting portion and extends into the barrel.

[0011] According to a multi-channel tissue sampler provided by the present invention, the push rod comprises a main push rod, a mounting plate and a plurality of auxiliary push rods;

[0012] One end of the main push rod is in contact with the rotating member, and the other end of the main push rod is connected to the mounting plate. A plurality of auxiliary push rods are mounted on the mounting plate and are arranged in one-to-one correspondence with a plurality of sampling tubes.

[0013] According to a multi-channel tissue sampler provided by the present invention, a plurality of booster rods are sequentially spaced apart along the length direction of the mounting plate, and the distance between two adjacent booster rods is adjustable.

[0014] According to a multi-channel tissue sampler provided by the present invention, the mounting plate is provided with a guide rail, and each of the booster rods is slidably disposed on the guide rail via a corresponding slider.

[0015] According to a multi-channel tissue sampler provided by the present invention, a hanging plate is provided on the outer side surface of each sampling tube, a plurality of mounting grooves are provided on the inner wall of the gripping rod, the height direction of each mounting groove is consistent with the height direction of the booster rod, and the hanging plate is fixed in the mounting groove by a magnetic attraction part.

[0016] According to a multi-channel tissue sampler provided by the present invention, the main push rod is a stepped structural member, a stopper is arranged inside the gripping rod, and the elastic member is sleeved on the outer side surface of the main push rod and limitedly located between the step surface of the main push rod and the stopper.

[0017] According to a multi-channel tissue sampler provided by the present invention, the gripping rod includes a gripping portion and a storage portion, the gripping portion and the storage portion are connected, the mounting plate and the sampling tube are located in the storage portion; the storage portion includes a top plate, a bottom plate, a first side plate, a second side plate, a first panel and a second panel, the top plate, the mounting plate and the bottom plate are arranged in parallel, and the top plate, the mounting plate and the bottom plate are all arc-shaped plates, and the first panel and the second panel are both fan-shaped plates.

[0018] The present invention also provides a sampling method of a multi-channel tissue sampler, comprising:

[0019] After the animals were anesthetized, the skull was removed and the whole monkey brain was obtained;

[0020] The monkey brain is quickly frozen by using dry ice and isopentane in sequence;

[0021] The quick-frozen monkey brain was packaged and transferred to a -70°C freezer;

[0022] Before sampling, the monkey brain in the refrigerator is placed in a freezing section machine box;

[0023] Adjusting the height and spacing of the sampling tubes and taking samples;

[0024] Save and label the sample.

[0025] The multi-channel tissue sampler and the sampling method provided by the present invention adjust the distance between two adjacent sampling tubes and the height of each sampling tube relative to the grip rod so that the sampling tube is adapted to the curvature of the cerebral cortex, insert the adjusted multi-channel tissue sampler into the brain tissue to a certain depth, and then pull it upward to complete the sampling. After the sampling is completed, the pressing piece is pressed to make the push rod push out the sample in each sampling tube. After the sample collection is completed, the pressing piece is pressed again to reset the push rod. In this way, intensive sampling can be completed in a short time. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 It is a structural schematic diagram of the multi-channel tissue sampler provided by the present invention.

[0028] Figure 2 It is a structural schematic diagram of the multi-channel tissue sampler provided by the present invention (without the gripping rod).

[0029] Figure 3 It is a schematic diagram of the internal structure of the storage part provided by the present invention.

[0030] Figure 4 It is a structural schematic diagram of the first panel provided by the present invention.

[0031] Figure 5 It is a structural schematic diagram of the sliding block provided by the present invention.

[0032] Figure 6 It is a structural schematic diagram of the booster rod provided by the present invention.

[0033] Figure 7 It is a structural schematic diagram of the rotating part provided by the present invention.

[0034] Figure 8 It is a structural schematic diagram of the pressing piece provided by the present invention.

[0035] Fig. 9 It is a structural schematic diagram of the mounting plate provided by the present invention.

[0036] Fig.10 It is a structural schematic diagram of the gripping portion provided by the present invention.

[0037] Fig.11 It is a structural schematic diagram of the sampling tube provided by the present invention.

[0038] Fig.12 It is a structural schematic diagram of the base plate provided by the present invention.

[0039] Fig.13 The present invention provides a flow chart of a sampling method for a multi-channel tissue sampler.

[0040] Reference numerals:

[0041] 1. Grip; 11. Grip portion; 111. Ring body; 112. Guide groove; 113. Inclined groove; 12. Storage portion; 121. Top plate; 122. First panel; 1221. Mounting groove; 123. Bottom plate; 1231. Mounting hole; 2. Pressing assembly; 21. Pressing member; 211. Cylinder body; 212. First guide portion; 213. Tooth portion; 22. Rotating member; 221. Second guide portion; 222. Connecting portion; 223. Rotating portion; 3. Sampling cylinder; 31. Hanging plate; 4. Push rod; 41. Main push rod; 42. Mounting plate; 421. Guide rail; 43. Assisting rod; 44. Sliding block; 5. Elastic member. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Combine the following Figures 1 to 13 The multi-channel tissue sampler and the sampling method thereof of the present invention are described.

[0044] It should be pointed out that the volume of the macaque brain is about 50 times that of the mouse brain. Commonly used tissue sampling tools are single-hole and can only obtain one sample at a time. In other words, it is difficult for traditional tissue sampling tools to complete dense sampling of the entire cerebral cortex of macaques in a short period of time.

[0045] In order to solve the above problems, Figure 1 , Figure 2 , Fig.10 and Fig.11 As shown, the multi-channel tissue sampler according to the embodiment of the present invention comprises: a gripping rod 1 , a pushing rod 4 , an elastic member 5 , a pressing assembly 2 and a plurality of sampling barrels 3 .

[0046] The pressing assembly 2 includes a pressing member 21 and a rotating member 22. The pressing member 21, the rotating member 22 and the push rod 4 are installed in the grip 1 in sequence. The end of the pressing member 21 away from the rotating member 22 is exposed to the grip 1. The push rod 4 is movably arranged in the grip 1 through an elastic member 5. Multiple sampling barrels 3 can be detachably installed in the grip 1, and the sampling port of each sampling barrel 3 is exposed to the grip 1. The inner wall of the grip 1 is provided with a limiting portion that cooperates with the pressing member 21 and the rotating member 22.

[0047] Among them, the multi-channel tissue sampler has a sampling state and an extrusion state. In the sampling state, under the action of the elastic member 5, the pressing member 21 and the rotating member 22 are aligned and both are located at the limit portion at the upper limit of the axial direction of the grip 1; in the extrusion state, the pressing member 21 and the rotating member 22 are staggered, and the rotating member 22 abuts against the limit portion, and the push rod 4 extends into each sampling tube 3.

[0048] In actual application, the distance between two adjacent sampling tubes 3 and the height of each sampling tube 3 relative to the grip 1 are adjusted so that the sampling tube 3 matches the curvature of the cerebral cortex, and the adjusted multi-channel tissue sampler is inserted into the brain tissue to a certain depth, and then pulled upward to complete the sampling. After the sampling is completed, the pressing member 21 is pressed to make the push rod 4 push out the sample in each sampling tube 3. After the sample collection is completed, the pressing member 21 is pressed again to reset the push rod 4. In this way, intensive sampling can be completed in a short time.

[0049] like Figure 1 , Figure 2 , Figure 7 , Figure 8 and Fig.10 As shown, the pressing member 21 includes a cylinder 211, a tooth portion 213 and a first guide portion 212. The tooth portion 213 is provided at one end of the cylinder 211 facing the rotating member 22, and a plurality of first guide portions 212 are provided at intervals on the outer side surface of the cylinder 211 along its circumferential direction; the rotating member 22 includes a connecting portion 222 and a plurality of second guide portions 221 arranged in a circumference, two adjacent second guide portions 221 are connected by the connecting portion 222, and one end of each second guide portion 221 facing the tooth portion 213 is inclined; the limiting portion includes an annular body 111, a guide groove 112 and an inclined groove 113, the guide groove 112 and the inclined groove 113 are both provided on the annular body 111, a plurality of guide grooves 112 are provided at intervals along the circumferential direction of the annular body 111, and the inclined groove 113 is located between two adjacent guide grooves 112 and is connected to the notch of one of the guide grooves 112.

[0050] The first guide portion 212 may be provided in a rectangular structure, and the second guide portion 221 may also be provided in a rectangular structure. The first guide portion 212 and the second guide portion 221 have the same width, and both the first guide portion 212 and the second guide portion 221 are adapted to the guide groove 112 .

[0051] Specifically, when the cylinder 211 moves downward along the gripping rod 1, the first guide portion 212 of the pressing member 21 and the second guide portion 221 of the rotating member 22 are both in the guide groove 112 and move along the notch direction of the guide groove 112. When the second guide portion 221 of the rotating member 22 completely slides out of the guide groove 112, the rotating member 22 rotates along the first direction, and the second guide portion 221 of the rotating member 22 can be inserted into the tooth portion 213 of the pressing member 21. At the same time, the elastic member 5 is compressed, and the push rod 4 can enter the sampling cylinder 3 and push out the biological tissue in the sampling cylinder 3. After that, the cylinder 211 is released, and under the action of the elastic member 5, the second guide portion 221 of the rotating member 22 can be clamped in the inclined groove 113 at the end of the guide groove 112, and the push rod 4 can remain stationary; the cylinder 211 is pressed again, the elastic member 5 is compressed again, and the second guide portion 221 of the rotating member 22 is disengaged from the inclined groove 113 at the end of the guide groove 112, and the rotating member 22 can rotate along the second direction, and under the action of the elastic member 5, the second guide portion 221 of the rotating member 22 can slide into the guide groove 112 again, and the push rod 4 is reset. The first direction and the second direction are opposite. For example, the first direction is clockwise, and the second direction is counterclockwise.

[0052] In practical applications, in order to ensure the stability of the movement of the pressing member 21 and the rotating member 22, as shown in FIG. Figure 1 , Figure 2 , Figure 7 , Figure 8 and Fig.10 As shown, the rotating member 22 further includes a rotating portion 223, which is connected to the connecting portion 222 and extends into the cylinder 211. It should be noted that the rotating portion 223, the connecting portion 222 and the second guide portion 221 can be an integrated structure.

[0053] In an optional embodiment, if Figure 1 and Figure 2 As shown, the push rod 4 includes a main push rod 41, a mounting plate 42 and a plurality of booster rods 43. One end of the main push rod 41 abuts against the rotating member 22, and the other end of the main push rod 41 is connected to the mounting plate 42. The plurality of booster rods 43 are mounted on the mounting plate 42 and are arranged one by one corresponding to the plurality of sampling barrels 3. In order to avoid damage to the biological tissue in the sampling barrel 3, the booster rods 43 can be made of silicone.

[0054] In practical applications, multiple booster rods 43 are sequentially arranged at intervals along the length direction of the mounting plate 42, and the distance between two adjacent booster rods 43 is adjustable. In other words, the position of the booster rod 43 can be adjusted in real time according to the adjusted position of the sampling barrel 3, so that the sampling barrel 3 corresponds to the booster rod 43 one by one.

[0055] like Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 and Fig. 9 As shown, the mounting plate 42 is provided with a guide rail 421, and each booster rod 43 is slidably disposed on the guide rail 421 through a corresponding slider 44. It should be noted that the bottom surface of the mounting plate 42 is provided with a guide rail 421, and a plurality of sliders 44 are all slidably matched with the guide rail 421, and each slider 44 is connected to a corresponding booster rod 43.

[0056] Specifically, the booster rod 43 can be an L-shaped structural member, a through hole is set on the slider 44, the short side of the booster rod 43 is passed through the through hole, and the short side of the booster rod 43 is provided with a thread. In this way, the booster rod 43 and the slider 44 can be fixed by a bolt threadedly connected to the short side of the booster rod 43.

[0057] like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Fig.11 As shown, a hanging plate 31 is provided on the outer side of each sampling tube 3, and a plurality of mounting grooves 1221 are provided on the inner wall of the gripping rod 1. The height direction of each mounting groove 1221 is consistent with the height direction of the boosting rod 43, and the hanging plate 31 is fixed in the mounting groove 1221 by a magnetic attraction member. The hanging plate 31 may be vertically placed in a Z shape.

[0058] It should be noted that the sampling tube 3 is a stepped structural member, and the material of the sampling tube 3 can be selected from metal iron, and its surface is electroplated and oxidized. A mounting plate 31 is provided on the top of the sampling tube 3, and is mounted in the mounting groove 1221 by magnetic attraction. For example, a strong magnet is provided at a position corresponding to the back of the mounting groove 1221. Before sampling, the spacing between the sampling tubes 3 and the height of the sampling tubes 3 are adjusted according to the needs, and each sampling tube 3 is made perpendicular to the plane of the sampling site as much as possible.

[0059] In practical applications, the relative height of the end of each sampling tube 3 can be adjusted separately, so as to realize single-time multi-site and equal-depth sampling of non-planar tissues to be sampled. The spacing between the sampling tubes 3 can be adjusted, and the sampling density can be adjusted according to needs; biological tissues can be separated from multiple sampling tubes 3 at the same time; the sampling tubes 3 can be installed or removed according to needs to control the number of samples. In this way, the efficiency of tissue sampling can be significantly improved, which is particularly suitable for high-density and rapid sampling of non-planar tissues such as the cerebral cortex of large animals.

[0060] In an optional embodiment, in order to facilitate the installation of the elastic member 5, the main push rod 41 is a stepped structure, a stopper is provided inside the handle 1, and the elastic member 5 is sleeved on the outer side of the main push rod 41 and limited between the step surface of the main push rod 41 and the stopper. The stopper may be an annular stopper, so that the main push rod 41 can be installed on the annular stopper. In addition, the elastic member 5 may be a spring.

[0061] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Fig.10 , Fig.11 and Fig.12 As shown, the gripping rod 1 includes a gripping portion 11 and a storage portion 12, the gripping portion 11 and the storage portion 12 are connected, the mounting plate 42 and the sampling tube 3 are located in the storage portion 12; the storage portion 12 includes a top plate 121, a bottom plate 123, a first side plate, a second side plate, a first panel 122 and a second panel, the top plate 121, the mounting plate 42 and the bottom plate 123 are arranged in parallel, and the top plate 121, the mounting plate 42 and the bottom plate 123 are all arc-shaped plates, and the first panel 122 and the second panel are both fan-shaped plates.

[0062] It should be noted that the gripping portion 11 includes a first portion and a second portion, the first portion is connected to the receiving portion 12 via the second portion, wherein the second portion and the receiving portion 12 are an integrated structure, and the first portion and the second portion are threadedly connected. In addition, the first portion is provided with an opening, the cylinder 211 is passed through the opening, a limiting portion is provided on the inner wall of the first portion, and an annular stopper is provided on the inner wall of the second portion.

[0063] It is understandable that the bottom plate 123 is provided with a through hole 1231, the sampling tube 3 is passed through the through hole 1231, and the first panel 122 is provided with a mounting groove 1221. In addition, in order to facilitate the adjustment of the sampling tube 3 and the booster rod 43, part of the storage portion 12 is detachable, for example, the first side plate, the second panel and the bottom plate 123 are detachable, or the first side plate and the second panel are detachable.

[0064] The multi-channel tissue sampler of the embodiment of the present invention is suitable for sampling of large samples, especially the whole brain cortex tissue proteome or transcriptome of macaques before sequencing. In addition, the surface of the macaque brain is a curved surface, and the height of the sampling tube 3 can be adjusted to make the sample depth consistent each time, thereby improving the sampling efficiency and ensuring the consistency of the sample depth.

[0065] It is particularly important to point out that an inappropriate sampling process may directly affect the quality of the data obtained. During the tissue sampling process, if fresh tissue is left at room temperature for a long time, the RNA and protein in the tissue will degrade, which will directly affect the quality of the data obtained and fail to reflect the true gene expression of the tissue under a specific physiological state. In order to avoid RNA or protein degradation, the time that fresh tissue is left at room temperature should be shortened as much as possible.

[0066] In addition, if Fig.13 As shown, the sampling method of the multi-channel tissue sampler of the embodiment of the present invention includes:

[0067] At S100, the animals were anesthetized, the skull was removed, and the whole monkey brain was obtained.

[0068] S200, monkey brains were quickly frozen using dry ice and isopentane in sequence.

[0069] S300, wrap the quick-frozen brain and transfer it to a -70°C freezer.

[0070] S400, before sampling, the monkey brain in the refrigerator is placed into the freezing section machine box.

[0071] S500, adjust the height and spacing of the sampling tubes and take samples.

[0072] S600, save and label the sample.

[0073] The following is a specific explanation using the inferior parietal lobule area of ​​the monkey brain as an example.

[0074] After the monkeys were anesthetized, 3T MRI was used to scan the monkey brain to obtain MRI images;

[0075] Print a 3D monkey brain mold in proportion based on MRI images;

[0076] With the help of the monkey brain 3D mold, the spacing and height of the sampling tubes were adjusted to fit the inferior parietal lobule cortex of the monkey brain;

[0077] After anesthesia, the skull was removed to obtain the complete monkey brain;

[0078] The monkey brain was quickly frozen using dry ice and -70°C isopentane, respectively;

[0079] Wrap the monkey brain in a fresh-keeping bag and transfer it to a refrigerator set at -70°C;

[0080] Three hours before sampling, the monkey brain was placed in a freezing section machine cabinet set at −20°C;

[0081] Use a multi-channel tissue sampler perpendicular to the cortex and allow the sampling tube to enter the biological tissue, wherein the sampling tube can be inserted to a depth of about 2.5 mm;

[0082] Pull the sampling tube upwards, align the sampling tube with the marked EP tubes, press the pressing piece to push out the sample, so that the sample enters the corresponding EP tube.

[0083] The sampling method of the multi-channel tissue sampler of the embodiment of the present invention is suitable for fresh tissue, large-scale, intensive sampling, and is particularly suitable for experiments with strict requirements on the degradation of proteins or RNA in the samples. By combining fresh tissue freezing and sampling at -20°C, not only the effective sampling time is extended, but also the sample hardness is increased, reducing the difficulty of sampling. In other words, the sampling efficiency can be greatly improved and the degradation risk of RNA and protein can be further reduced.

[0084] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-channel tissue sampler, characterized in that: include: A grip rod, a push rod, an elastic member, a pressing assembly, and a plurality of sampling tubes; The pressing assembly comprises a pressing member and a rotating member, wherein the pressing member, the rotating member and the push rod are sequentially installed in the gripping rod, one end of the pressing member away from the rotating member is exposed to the gripping rod, the push rod is movably arranged in the gripping rod through the elastic member, a plurality of sampling barrels can be detachably installed in the gripping rod, and a sampling port of each sampling barrel is exposed to the gripping rod, and a limiting portion cooperating with the pressing member and the rotating member is arranged on the inner wall of the gripping rod; The multi-channel tissue sampler has a sampling state and a squeezing state. In the sampling state, under the action of the elastic member, the pressing member and the rotating member are aligned and both are located at the upper limit of the axial direction of the gripping rod at the limit portion; in the squeezing state, the pressing member and the rotating member are staggered, and the rotating member abuts against the limit portion, and the push rod extends into each of the sampling barrels; The push rod comprises a main push rod, a mounting plate and a plurality of booster rods; One end of the main push rod is in contact with the rotating member, and the other end of the main push rod is connected to the mounting plate. A plurality of auxiliary push rods are mounted on the mounting plate and are arranged one by one corresponding to a plurality of sampling tubes. The main push rod is a stepped structural member, a stopper is arranged inside the grip rod, and the elastic member is sleeved on the outer side surface of the main push rod and is limited to be located between the step surface of the main push rod and the stopper; A plurality of booster rods are sequentially arranged at intervals along the length direction of the mounting plate, and the distance between two adjacent booster rods is adjustable; the mounting plate is provided with a guide rail, and each booster rod is slidably arranged on the guide rail through a corresponding slider; The outer side surface of each sampling tube is provided with a hanging plate, the inner wall of the gripping rod is provided with a plurality of mounting grooves, the height direction of each mounting groove is consistent with the height direction of the booster rod, and the hanging plate is fixed in the mounting groove by a magnetic attraction member.

2. The multi-channel tissue sampler according to claim 1, characterized in that: The pressing member comprises a cylinder, a tooth portion and a first guide portion, the cylinder is provided with the tooth portion at one end facing the rotating member, and a plurality of first guide portions are provided at intervals on the outer side surface of the cylinder along the circumferential direction thereof; The rotating member comprises a connecting portion and a plurality of second guide portions arranged in a circumference, two adjacent second guide portions are connected by the connecting portion, and one end of each second guide portion facing the tooth portion is inclined; The limiting portion includes an annular body, a guide groove and an oblique groove. The guide groove and the oblique groove are both arranged on the annular body. A plurality of the guide grooves are arranged at intervals along the circumferential direction of the annular body. The oblique groove is located between two adjacent guide grooves and is connected to the notch of one of the guide grooves.

3. The multi-channel tissue sampler according to claim 2, characterized in that: The rotating member further includes a rotating portion, which is connected to the connecting portion and extends into the cylinder.

4. The multi-channel tissue sampler according to claim 1, characterized in that: The gripping rod includes a gripping portion and a storage portion, the gripping portion and the storage portion are connected, and the mounting plate and the sampling tube are located in the storage portion; the storage portion includes a top plate, a bottom plate, a first side plate, a second side plate, a first panel and a second panel, the top plate, the mounting plate and the bottom plate are arranged in parallel, and the top plate, the mounting plate and the bottom plate are all arc-shaped plates, and the first panel and the second panel are both fan-shaped plates.

Images