Mycobacterium phenotype drug sensitivity detection device based on automation

Abstract

The invention discloses an automation-based mycobacterium phenotype drug sensitivity detection device in the technical field of medical detection, which comprises an automatic detection device and an automatic detection system which are in signal connection. The automatic detection device comprises a Raman spectrometer, a driving table and a downward pressing assembly used for pressing a glass slide, a carrying seat and a laser head located above the carrying seat are arranged in the Raman spectrometer, the downward pressing assembly comprises a pressing plate and a plurality of electric control telescopic rods fixedly connected to the two sides of the laser head, one end of each electric control telescopic rod is fixedly connected to the bottom of the laser head, and the other end of each electric control telescopic rod is fixedly connected to the driving table. The top of the pressing plate is fixedly connected with an output shaft of the electric control telescopic rod, and the pressing plate is provided with a light hole for laser to penetrate through the channel. According to the invention, a plurality of glass slides dropwise added with a mycobacterium culture solution are driven and rotated by the driving table placed in the Raman spectrometer, automatic phenotype drug sensitivity detection is carried out, and the glass slides are pressed by the pressing assembly and laser scattering is reduced, so that the detection result is more accurate.

Description

Technical Field

[0001] The invention belongs to the technical field of medical detection, and in particular is an automated mycobacterium phenotypic drug sensitivity detection device. Background Art

[0002] Mycobacteria are a class of bacteria that are widely present in the natural environment. Mycobacterium tuberculosis and fast-growing mycobacteria (RGM) have become important challenges in the field of global public health due to their high drug resistance and potential pathogenicity. These bacteria can cause a variety of serious diseases, such as tuberculosis and infections caused by RGM, and are difficult to treat and have a high recurrence rate. Therefore, rapid and accurate phenotypic drug sensitivity testing of mycobacteria is of great significance for guiding clinical drug use, optimizing treatment plans, and controlling the spread of drug-resistant bacteria. Phenotypic drug sensitivity testing can directly evaluate the sensitivity of bacteria to specific drugs and provide doctors with personalized treatment recommendations, thereby improving treatment outcomes, reducing unnecessary drug use, and reducing the development of drug resistance.

[0003] Traditional methods for mycobacterial drug sensitivity detection, such as Roche's absolute concentration method or ratio method, have significant drawbacks. These methods have a long detection cycle, usually requiring 50-70 days to obtain results, which is far from meeting the needs of rapid clinical diagnosis and treatment. In recent years, with the advancement of science and technology, the application of automation technology in the medical field has become increasingly widespread. Fully automatic mycobacterial culture monitoring systems, such as the BACTECMGIT960 system, achieve rapid growth and culture and drug sensitivity detection of mycobacteria by continuously detecting changes in fluorescence intensity in culture tubes. The system significantly shortens the detection cycle, improves detection efficiency, and provides a more timely and accurate basis for clinical diagnosis. However, in view of the high drug resistance of fast-growing mycobacteria (RGM), traditional methods are complicated to operate, difficult to standardize, and detection mostly relies on manual operation, which affects the accuracy and efficiency of detection.

[0004] A fast-growing mycobacterium drug sensitivity rapid detection device based on Raman spectroscopy technology (such as CAST-R-RGM), which realizes the rapid evaluation of drug sensitivity by detecting the stress response of cells under the action of drugs. Raman spectroscopy technology not only shortens the detection cycle to 24 hours, but also improves the accuracy and reliability of detection, providing strong support for clinical treatment. However, when using the existing Raman spectrometer to detect mycobacterium suspension, only the mycobacterium strain on one slide can be detected at a time, and the slide needs to be replaced frequently manually, which reduces the detection efficiency. Therefore, based on Raman spectroscopy technology, an automated mycobacterium phenotypic drug sensitivity detection device is designed, which is combined with the self-developed clinical drug sensitivity test model (CAST-R) instrument and the automated detection method to improve the detection efficiency and accuracy of mycobacterium phenotypic drug sensitivity. Summary of the invention

[0005] In order to solve the above problems, the purpose of the present invention is to drive and rotate several glass slides with mycobacterium culture fluid dropped on them through a driving table placed in a Raman spectrometer, and perform automated phenotypic drug sensitivity testing, and use a downward pressure component to press the glass slides and reduce laser scattering to make the test results more accurate.

[0006] In order to achieve the above-mentioned object, the technical scheme of the present invention is as follows: an automated mycobacterium phenotypic drug sensitivity detection device, comprising an automated detection device and an automated detection system, wherein the automated detection device is signal-connected to the automated detection system, the automated detection device comprises a Raman spectrometer, a driving platform and a pressing component for pressing a slide, a carrier and a laser head located above the carrier are provided in the Raman spectrometer, and the driving platform is placed on the carrier;

[0007] A driving assembly for providing power to the driving platform is provided in the driving platform, a transmission assembly is provided on one side of the driving assembly, a plurality of placement grooves are provided on the top of the side of the transmission assembly away from the driving assembly, the slides are installed in the placement grooves, and clamping assemblies for fixing the slides are provided on both sides of the placement grooves;

[0008] The pressing assembly includes a pressing plate and a plurality of electrically controlled telescopic rods fixedly connected to both sides of the laser head. One end of the electrically controlled telescopic rods is fixedly connected to the bottom of the laser head. The top of the pressing plate is fixedly connected to the output shaft of the electrically controlled telescopic rod. The pressing plate is provided with a light-transmitting hole for the laser to pass through the channel.

[0009] A base is placed at the bottom of the Raman spectrometer, and a first automatic adjustment component and a second automatic adjustment component connected to the signal of the automated detection system are fixedly connected on both sides of the base. A coarse adjustment knob and a fine adjustment knob for adjusting the focal length of the Raman spectrometer laser head are provided above the base. The first automatic adjustment component is clamped on the fine adjustment knob, and the second automatic adjustment component is clamped on the coarse adjustment knob.

[0010] The principle of the basic scheme is: by placing a glass slide with mycobacterium culture solution in the placement slot and fixing the glass slide with a clamping assembly, placing the driving platform with the glass slide on top of the object base, the automated detection system starts the driving assembly to provide power, and drives the placement slot with the glass slide to rotate through the transmission assembly.

[0011] At the same time, the first automatic adjustment component and the second automatic adjustment component on the base are clamped on the fine adjustment knob and the coarse adjustment knob respectively, which can automatically adjust the focal length of the laser head as needed to ensure that the laser focus always accurately falls on the culture solution on the slide, further improving the detection accuracy.

[0012] When the glass slide rotates to the bottom of the laser head, the electrically controlled telescopic rod in the downward pressure assembly drives the pressure plate downward, pressing the mycobacterium culture fluid on the glass slide into a thin sheet. The laser shines through the light-transmitting hole onto the glass slide with the mycobacterium culture fluid added. The photons interact with the molecules in the culture fluid to generate Raman scattered light. By detecting the frequency changes of the scattered light, the molecular vibration and rotation information of the mycobacteria in the culture fluid can be analyzed, and then its drug sensitivity characteristics can be determined.

[0013] The beneficial effects of the basic scheme are: 1. Automatic transmission and continuous detection process are realized through the driving components and transmission components on the driving table, which realizes the rapid detection of mycobacterium samples on multiple slides. There is no need to repeatedly open the box cover of the Raman detector to replace the slides, which shortens the detection time.

[0014] 2. The mycobacterium culture fluid on the slide is flattened by a pressure plate. When the sample is flattened, its surface becomes smoother, reducing the scattered light caused by the uneven sample. The reduction of scattered light means that more laser energy can directly act on the sample, thereby improving the intensity and clarity of the Raman spectrum signal. This helps to more accurately analyze the molecular vibration and rotation information of the sample and improve the accuracy of the test results.

[0015] 3. Flattening the sample can also improve the signal-to-noise ratio of the Raman spectroscopy signal. The signal-to-noise ratio is an important indicator to measure signal quality. A higher signal-to-noise ratio means that the useful information in the signal is more prominent and the noise interference is smaller. By flattening the sample, the background noise caused by the uneven surface of the sample can be reduced, thereby improving the signal-to-noise ratio and making the detection results more reliable.

[0016] 4. A flat sample surface is conducive to the collection and processing of Raman spectroscopy data. In the data processing stage, the flattened sample spectrum usually has a clearer peak shape and more accurate peak position, which makes the subsequent data analysis more accurate.

[0017] 5. The light-transmitting hole enables the laser to be quickly and accurately focused on the sample on the slide, reducing the scattering loss of the laser path, helping to reduce the signal quality degradation caused by beam distortion or uneven energy, thereby improving the accuracy and reliability of the test results.

[0018] 6. The focal length of the laser head is automatically adjusted by the first automatic adjustment component and the second automatic adjustment component, thereby ensuring the accuracy of the laser focus and further improving the detection accuracy.

[0019] Furthermore, the driving assembly includes a servo motor, which is fixedly connected to the inner bottom wall of the driving platform. The output shaft of the servo motor passes through the inner top wall of the driving platform and is fixedly connected to a boss-shaped active dial. The active dial is rotatably connected to the driving platform. An inwardly concave locking arc is provided on the active dial, and a cylindrical pin is fixedly connected to the active dial near the inwardly concave locking arc.

[0020] The beneficial effects of the basic solution are: through the high-precision positioning capability of the servo motor, it is possible to ensure that the active dial maintains extremely high position accuracy during rotation, which is crucial for detection devices that require precise control of the rotation angle and position of the slide, and helps to improve the accuracy and reliability of detection; the cylindrical pin and locking arc are designed to cooperate with the transmission component to achieve the transmission and locking functions.

[0021] Furthermore, the transmission assembly includes a driven groove wheel, which is rotatably connected to the top of the driving platform, and the groove of the driven groove wheel is rotationally matched with the outer wall of the locking arc. The protrusions of the driven groove wheel are all provided with radial grooves, and the radial grooves can contact and intermittently rotate with the cylindrical pins, and the placement grooves are respectively located on the upper part of the radial grooves.

[0022] The beneficial effects of the basic scheme are: the groove of the driven groove wheel is rotationally connected to the outer wall of the locking arc, and the cylindrical pin and the radial groove of the driven groove wheel intermittently rotate together, so that the slide can stay at the laser head position for a period of time for detection during rotation, without the need to repeatedly start and shut down the servo motor, thereby improving the efficiency of automated detection and reducing component loss.

[0023] Furthermore, the clamping assemblies include a pushing block, a movable cavity and a clamping clamp. The movable cavities are arranged in the radial groove on both sides close to the placement groove. One side of the pushing block is located in the placement groove and is slidably connected to the placement groove. The other side of the pushing block is located in the movable cavity and is fixedly connected with a rack. The end of the rack away from the pushing block is meshed with a gear. The center of the gear is sleeved with a rotating shaft. The end of the rotating shaft away from the gear passes through the movable cavity, extends to the top of the radial groove, and is fixedly connected to the clamping clamp.

[0024] The beneficial effects of the basic scheme are: through the sliding connection of the push block in the placement groove and the meshing transmission of the rack and the gear, the clamping clamp can be accurately moved to the specified position to stably fix the slide, reducing the process of manual operation to clamp the slide, ensuring the stability of the sample during the detection process, and avoiding detection errors caused by sample movement.

[0025] Furthermore, a weighing sensor is provided on the object carrier, and the weighing sensor is connected to the signal of the automatic detection system.

[0026] The beneficial effect of the basic solution is that the weighing sensor can sense in real time whether a driving platform is placed on the carrier, and transmit the signal to the automatic detection system to start other devices, thus ensuring the initial operation of the automatic detection device.

[0027] Furthermore, a plurality of support plates are fixedly connected to the top of the base, and a first motor and a second motor are fixedly connected to the support plates respectively. The first motor and the second motor are fixedly connected to the first automatic adjustment component and the second automatic adjustment component respectively.

[0028] The beneficial effects of the basic solution are: several support plates fixedly connected on the base provide stable support for the first motor and the second motor, and the first automatic adjustment component and the second automatic adjustment component are respectively controlled by the first motor and the second motor, so that the coarse adjustment and fine adjustment of the laser focal length do not affect each other, thereby improving the adjustment accuracy and flexibility of the system.

[0029] Furthermore, the first automatic adjustment component includes a first clamping claw, which is clamped on the fine-tuning knob. The side of the first clamping claw away from the fine-tuning knob is coaxially fixedly connected to the output shaft of the first motor, and the first motor is connected to the automatic detection system signal.

[0030] The beneficial effects of the basic solution are: the first motor is connected to the automatic detection system signal, realizing automatic control of the fine-tuning knob. Through a preset program or real-time feedback signal, the system can automatically adjust the position of the fine-tuning knob. The automatic adjustment reduces the user's manual operation and makes the adjustment process simpler and more intuitive.

[0031] Furthermore, the second automatic adjustment component includes a second clamping claw, which is clamped on the coarse adjustment knob. The side of the second clamping claw away from the coarse adjustment knob is coaxially fixedly connected to the second motor output shaft, and the second motor is connected to the automatic detection system signal.

[0032] The beneficial effect of the basic solution is that the second clamping claw is clamped on the coarse adjustment knob, so that the second motor can drive the coarse adjustment knob to make a large-scale position adjustment.

[0033] Furthermore, the inner walls of the first clamping claw and the second clamping claw are both provided with a flexible layer.

[0034] The beneficial effects of the basic solution are: the flexible layer avoids direct contact between the first clamping claw and the fine-tuning knob and between the second clamping claw and the coarse-tuning knob, thereby avoiding damage or scratches caused by hard contact during the clamping rotation process. At the same time, the flexible layer can increase the contact area and adhesion between the first clamping claw and the fine-tuning knob and between the second clamping claw and the coarse-tuning knob, thereby improving the stability of grasping.

[0035] Further, the automated detection system includes a receiving module, a control module, an identification module, a script module and an analysis module;

[0036] The receiving module is used to receive the weight of the weighing sensor on the object carrier and control the automatic detection device through the control module, and is used to collect the spectrum information of Raman scattered light;

[0037] The control module is used to control the Raman spectrometer, the electric control telescopic rod, the servo motor, the first motor and the second motor in the automated detection device;

[0038] The recognition module is used to irradiate the mycobacterium species on the slide with the laser emitted by the laser head;

[0039] The script module is used to perform background noise subtraction, baseline correction, normalization preprocessing on the obtained Raman spectral data, and filter low-quality spectra;

[0040] The analysis module is used to analyze the Raman spectral data processed by the script module to obtain the vibration and rotation information of the molecules in the sample, and to derive the sensitivity of mycobacteria to phenotypic drugs through characteristic parameters such as peak position, peak intensity and peak shape in the spectrum.

[0041] The beneficial effects of the basic scheme are: 1. The receiving module receives the weight information of the weighing sensor on the load cell, controls the automatic detection device to collect Raman spectra, and then analyzes and processes the spectral information, all of which are highly automated. This not only reduces the tediousness and errors of manual operation, but also greatly improves the detection efficiency.

[0042] 2. The control module can quickly respond to the instructions from the receiving module and accurately control the operation of the Raman spectrometer, electric telescopic rod, servo motor and other equipment to ensure the smoothness and efficiency of the detection process.

[0043] 3. The recognition module accurately irradiates the mycobacterium species on the slide with the laser emitted by the laser head, ensuring the accuracy and pertinence of the spectral information collection.

[0044] 4. The script module improves the accuracy of spectral information by processing the noise interference that occurs when laser identifies mycobacterial susceptibility information and filtering low-quality spectra that are difficult to analyze.

[0045] 5. The analysis module uses advanced algorithms to conduct in-depth analysis of the spectral information of Raman scattered light, and accurately obtains the sensitivity of mycobacteria to phenotypic drugs through characteristic parameters such as peak position, peak intensity and peak shape in the spectrum. This analysis method based on spectral information is more accurate and reliable than traditional drug sensitivity test methods.

[0046] 6. The automated detection system achieves efficient and accurate detection of the phenotypic drug sensitivity of Mycobacterium by integrating the receiving module, control module, identification module, script module and analysis module. It has the effects of improving detection efficiency, enhancing detection accuracy, reducing manual intervention, and having strong adaptability and flexibility. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1It is an axonometric schematic diagram of a driving platform and a pressing component of an automated Mycobacterium phenotypic drug sensitivity detection device in an embodiment of the present invention.

[0048] Figure 2 It is an axonometric schematic diagram of the clamping assembly of the automated Mycobacterium phenotypic drug sensitivity detection device in an embodiment of the present invention.

[0049] Figure 3 It is a front cross-sectional view of the clamping assembly of the automated Mycobacterium phenotypic drug sensitivity detection device in an embodiment of the present invention.

[0050] Figure 4 It is a front cross-sectional view of the bottom position of the Raman spectrometer of the automated mycobacterium phenotypic drug sensitivity detection device in an embodiment of the present invention.

[0051] The figure marks in the drawings of the specification include: 1. laser head; 2. electric telescopic rod; 3. light-transmitting hole; 4. pressure plate; 5. driving platform; 6. driven groove wheel; 7. clamping clamp; 8. rotating shaft; 9. active dial; 10. locking arc; 11. cylindrical pin; 12. pushing block; 13. placement groove; 14. radial groove; 15. rack; 16. gear; 17. movable cavity; 18. fine-tuning knob; 19. first clamping claw; 20. first motor; 21. support plate; 22. base; 23. coarse-adjusting knob; 24. second clamping claw; 25. second motor. DETAILED DESCRIPTION

[0052] The following is further described in detail through specific implementation methods:

[0053] Embodiment 1:

[0054] Basically as attached Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown: an automated Mycobacterium phenotypic drug sensitivity detection device, including an automated detection device and an automated detection system, the automated detection device is signal-connected to the automated detection system, the automated detection device includes a Raman spectrometer, a drive platform 5 and a pressing component for pressing a slide, a carrier and a laser head 1 located above the carrier are provided in the Raman spectrometer, the drive platform 5 is placed on the carrier, a weighing sensor is provided on the carrier, and the weighing sensor is signal-connected to the automated detection system.

[0055] A driving assembly for providing power to the driving platform 5 is provided in the driving platform 5, and the driving assembly includes a servo motor, and the servo motor is bolted to the inner bottom wall of the driving platform 5. The output shaft of the servo motor passes through the inner top wall of the driving platform 5 and is welded with a boss-shaped active dial 9, and the active dial 9 is rotatably connected to the driving platform 5. A concave locking arc 10 is provided on the active dial 9, and a cylindrical pin 11 is welded to the concave part of the active dial 9 near the locking arc 10. A transmission assembly is provided on one side of the active dial 9, and the transmission assembly includes a driven groove wheel 6, which is rotatably connected to the top of the driving platform 5, and the groove of the driven groove wheel 6 is rotatably matched with the outer side wall of the locking arc 10, and the protrusions of the driven groove wheel 6 are provided with radial grooves 14, and the radial grooves 14 can contact the cylindrical pin 11 And intermittently rotate together, a plurality of placement grooves 13 are opened on the upper part of the radial groove 14, the slide is installed in the placement groove 13, and clamping components for fixing the slide are provided on both sides of the placement groove 13. The clamping components include a pushing block 12, an active cavity 17 and a clamping clamp 7. The active cavity 17 is arranged on both sides of the radial groove 14 close to the placement groove 13. One side of the pushing block 12 is located in the placement groove 13 and is slidably connected to the placement groove 13. The other side of the pushing block 12 is located in the active cavity 17 and is welded with a rack 15. The end of the rack 15 away from the pushing block 12 is meshed with a gear 16, and the center of the gear 16 is sleeved with a rotating shaft 8. The end of the rotating shaft 8 away from the gear 16 passes through the active cavity 17, extends to the top of the radial groove 14, and is welded to the clamping clamp 7.

[0056] The pressing assembly includes a pressing plate 4 and several electrically controlled telescopic rods bolted to both sides of the laser head 1. One end of the electrically controlled telescopic rods is bolted to the bottom of the laser head 1. The pressing plate 4 is welded to the other end of the electrically controlled telescopic rods. The pressing plate 4 is provided with a light-transmitting hole 3 for the laser to pass through the channel.

[0057] A base 22 is placed at the bottom of the Raman spectrometer, and a first automatic adjustment component and a second automatic adjustment component connected to the signal of the automated detection system are bolted to both sides of the base 22. A coarse adjustment knob 23 and a fine adjustment knob 18 for adjusting the focal length of the Raman spectrometer laser head 1 are provided above the base 22. The first automatic adjustment component is clamped on the fine adjustment knob 18, and the second automatic adjustment component is clamped on the coarse adjustment knob 23.

[0058] The specific implementation process is as follows: the mycobacterium isolates and strains are cultured and prepared into a bacterial suspension, and a glass slide with the mycobacterium suspension is inserted into the placement groove 13 in parallel. After the glass slide enters the placement groove 13, the pushing block 12 is squeezed to slide into the active cavity 17. The rack 15 is engaged with the gear 16 under the action of the pushing block 12 to drive the rotating shaft 8 to rotate, so that the clamping clamp 7 rotates above the glass slide to clamp and fix the glass slide, and the driving platform 5 with the glass slide is placed above the carrier. The weighing sensor on the carrier senses the weight of the driving platform 5 and transmits a signal to the automatic detection system. The automatic detection system first drives the first automatic adjustment component and the second automatic adjustment component to adjust the focal length of the laser head 1.

[0059] When the focal length of the laser head 1 is adjusted, the automatic detection system drives the servo motor to rotate, and then transmits it to the driven groove wheel 6 through the active dial 9. When the cylindrical pin 11 is rotatably connected with the radial groove 14, the cylindrical pin 11 drives the driven groove wheel 6 to rotate, and rotates the glass slide to the bottom of the laser head 1. At this time, the locking arc 10 and the groove of the driven groove wheel 6 rotate to cooperate, so that the glass slide can stay at the position of the laser head 1 for a period of time for detection when the servo motor drives it to rotate.

[0060] When the glass slide moves to the laser irradiation position, the automated detection system controls the electric telescopic rod 2 to extend downward, so that the pressing plate 4 presses the mycobacterium culture solution on the glass slide and presses it into a thin layer. The purpose of this step is to make the sample surface flat. The flat sample surface reduces the scattering of light in uneven places, allowing more laser energy to be focused directly on the sample. In this way, the intensity of the Raman spectroscopy signal is enhanced, and the signal becomes clearer, because the reduction of scattered light allows the laser energy to interact with the sample more effectively.

[0061] In addition, flattening the sample also helps improve the quality of the Raman spectrum signal, that is, the signal-to-noise ratio. As the sample surface becomes flat, the additional noise caused by the uneven surface is reduced, making the signal in the spectrum purer and the background noise lower. Therefore, the peak shape on the spectrum is clearer and the peak position is more accurate, providing more accurate basic data for subsequent data processing and analysis, thereby improving the accuracy and reliability of the entire detection process.

[0062] At the same time, the laser is irradiated onto the glass slide with the mycobacterium culture solution through the light-transmitting hole 3 on the pressure plate 4. The light-transmitting hole 3 enables the laser to be quickly and accurately focused on the sample on the glass slide, reducing the scattering loss of the laser path, and helping to reduce the signal quality degradation problem caused by beam distortion or energy unevenness, thereby improving the accuracy and reliability of the detection results.

[0063] When the laser photons interact with the molecules in the culture medium, Raman scattered light is generated. By detecting the frequency changes of these scattered lights, the molecular vibration and rotation information of the mycobacteria in the culture medium can be analyzed, and then its drug sensitivity characteristics can be determined.

[0064] Embodiment 2:

[0065] The difference from the above embodiment is that the Figure 4 As shown: a plurality of support plates 21 are welded on the top of the base 22, and the first motor 20 and the second motor 25 are bolted to the support plates 21 respectively. The first automatic adjustment component includes a first clamping claw 19, and the first clamping claw 19 is clamped on the fine-tuning knob 18. The side of the first clamping claw 19 away from the fine-tuning knob 18 is coaxially welded to the output shaft of the first motor 20, and the first motor 20 is connected to the signal of the automatic detection system.

[0066] The second automatic adjustment component includes a second clamping claw 24, which is clamped on the coarse adjustment knob 23. The side of the second clamping claw 24 away from the coarse adjustment knob 23 is coaxially welded with the output shaft of the second motor 25. The second motor 25 is connected to the signal of the automatic detection system. The inner walls of the first clamping claw 19 and the second clamping claw 24 are both provided with a flexible layer.

[0067] The specific implementation process is as follows: the support plates 21 welded on the base 22 provide a stable support for the first motor 20, the first motor 20 is connected to the automatic detection system signal through a signal line, and the first motor 20 is started according to the real-time feedback signal of the weighing sensor, thereby automatically adjusting the precise position of the fine-tuning knob 18. This method reduces the manual operation of the user, makes the adjustment process simpler and faster, and the result is more accurate.

[0068] The second clamping claw 24 is designed to clamp the coarse adjustment knob 23, so that the second motor 25 can drive the coarse adjustment knob 23 to adjust the focal length. The separate control of the first motor 20 and the second motor 25 makes the coarse adjustment and fine adjustment processes independent of each other, thereby improving the adjustment accuracy and system flexibility.

[0069] The flexible layer avoids direct contact between the first clamping claw 19 and the fine-tuning knob 18 and between the second clamping claw 24 and the coarse-tuning knob 23, thereby avoiding damage or scratches caused by hard contact during the clamping rotation process. At the same time, the flexible layer can increase the contact area and adhesion between the first clamping claw 19 and the fine-tuning knob 18 and between the second clamping claw 24 and the coarse-tuning knob 23, thereby improving the stability of grasping.

[0070] Embodiment 3:

[0071] The difference from the above embodiment is that the automatic detection system includes a receiving module, a control module, an identification module, a script module and an analysis module.

[0072] The receiving module is used to receive the weight of the weighing sensor on the object carrier and control the automatic detection device through the control module, and is used to collect the spectrum information of Raman scattered light.

[0073] The control module is used to control the Raman spectrometer, the electrically controlled telescopic rod, the servo motor, the first motor 20 and the second motor 25 in the automated detection device.

[0074] The identification module is used to irradiate the mycobacterium species on the slide with the laser emitted by the laser head 1.

[0075] The script module is used to preprocess the obtained Raman spectral data by background noise subtraction, baseline correction, normalization, and filtering low-quality spectra.

[0076] The analysis module is used to analyze the Raman spectral data processed by the script module to obtain the vibration and rotation information of the molecules in the sample, and to derive the sensitivity of mycobacteria to phenotypic drugs through characteristic parameters such as peak position, peak intensity and peak shape in the spectrum.

[0077] The specific implementation process is as follows: the receiving module receives the weight information of the weighing sensor on the carrier, controls the automatic detection device to collect Raman spectra, and then analyzes and processes the spectral information, all of which are highly automated. This not only reduces the tediousness and errors of manual operation, but also greatly improves the detection efficiency.

[0078] The control module can quickly respond to instructions from the receiving module and accurately control the operation of equipment such as the Raman spectrometer, the electric telescopic rod, and the servo motor to ensure a smooth and efficient detection process.

[0079] The recognition module precisely irradiates the mycobacterium species on the slide with the laser emitted by the laser head 1, ensuring the accuracy and pertinence of the spectral information collection.

[0080] The script module is used to process the noise interference that occurs when laser identification of mycobacterial susceptibility information is performed and filter out low-quality spectra that are difficult to analyze, thereby improving the accuracy of spectral information.

[0081] The analysis module uses advanced algorithms to conduct in-depth analysis of the spectral information of Raman scattered light, and accurately obtains the sensitivity of mycobacteria to phenotypic drugs through characteristic parameters such as peak position, peak intensity and peak shape in the spectrum. This analysis method based on spectral information is more accurate and reliable than traditional drug sensitivity test methods.

[0082] This automated detection system achieves efficient and accurate detection of the phenotypic drug sensitivity of Mycobacterium by integrating a receiving module, a control module, an identification module, a script module and an analysis module. It has the effects of improving detection efficiency, enhancing detection accuracy, reducing manual intervention, and having strong adaptability and flexibility.

[0083] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0084] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An automated Mycobacterium phenotypic drug sensitivity detection device, characterized in that: The invention comprises an automated detection device and an automated detection system, wherein the automated detection device is connected to the automated detection system by signals, wherein the automated detection device comprises a Raman spectrometer, a drive platform (5) and a pressing component for pressing a glass slide, wherein a carrier and a laser head (1) located above the carrier are provided in the Raman spectrometer, and the drive platform (5) is placed on the carrier; A driving assembly for providing power to the driving platform (5) is provided in the driving platform (5), a transmission assembly is provided on one side of the driving assembly, a plurality of placement grooves (13) are provided on the top of a side of the transmission assembly away from the driving assembly, a glass slide is installed in the placement groove (13), and clamping assemblies for fixing the glass slide are provided on both sides of the placement groove (13); The pressing assembly comprises a pressing plate (4) and a plurality of electrically controlled telescopic rods fixedly connected to both sides of the laser head (1), one end of each electrically controlled telescopic rod being fixedly connected to the bottom of the laser head (1), the top of the pressing plate (4) being fixedly connected to the output shaft of the electrically controlled telescopic rod, and a light-transmitting hole (3) for the laser to pass through the channel is opened on the pressing plate (4); A base (22) is placed at the bottom of the Raman spectrometer, and a first automatic adjustment component and a second automatic adjustment component connected to the signal of the automated detection system are fixedly connected on both sides of the base (22), and a coarse adjustment knob (23) and a fine adjustment knob (18) for adjusting the focal length of a Raman spectrometer laser head (1) are arranged above the base (22), the first automatic adjustment component is clamped on the fine adjustment knob (18), and the second automatic adjustment component is clamped on the coarse adjustment knob (23).

2. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 1, characterized in that: The driving assembly comprises a servo motor, the servo motor is fixedly connected to the inner bottom wall of the driving platform (5), the output shaft of the servo motor passes through the inner top wall of the driving platform (5) and is fixedly connected to a boss-shaped active dial (9), the active dial (9) is rotatably connected to the driving platform (5), a concave locking arc (10) is provided on the active dial (9), and a cylindrical pin (11) is fixedly connected to the active dial (9) near the concave part of the locking arc (10).

3. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 2, characterized in that: The transmission assembly comprises a driven groove wheel (6), the driven groove wheel (6) is rotatably connected to the top of the driving platform (5), and the groove of the driven groove wheel (6) is rotatably matched with the outer wall of the locking arc (10), the protrusion of the driven groove wheel (6) is provided with a radial groove (14), the radial groove (14) and the cylindrical pin (11) can contact and intermittently rotate, and the placement groove (13) is respectively located at the upper part of the radial groove (14).

4. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 3, characterized in that: The clamping components each comprise a pushing block (12), an active cavity (17) and a clamping clip (7). The active cavity (17) is arranged in the radial groove (14) on both sides close to the placement groove (13). One side of the pushing block (12) is located in the placement groove (13) and is slidably connected to the placement groove (13). The other side of the pushing block (12) is located in the active cavity (17) and is fixedly connected to a rack (15). An end of the rack (15) away from the pushing block (12) is meshed with a gear (16). A rotating shaft (8) is sleeved at the center of the gear (16). An end of the rotating shaft (8) away from the gear (16) passes through the active cavity (17) and extends to the top of the radial groove (14) and is fixedly connected to the clamping clip (7).

5. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 4, characterized in that: A weighing sensor is arranged on the object carrier, and the weighing sensor is connected with the signal of the automatic detection system.

6. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 5, characterized in that: A plurality of support plates (21) are fixedly connected to the top of the base (22); a first motor (20) and a second motor (25) are fixedly connected to the support plates (21); and the first motor (20) and the second motor (25) are fixedly connected to the first automatic adjustment component and the second automatic adjustment component, respectively.

7. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 6, characterized in that: The first automatic adjustment component comprises a first clamping claw (19), the first clamping claw (19) is clamped on the fine-tuning knob (18), the side of the first clamping claw (19) away from the fine-tuning knob (18) is coaxially fixedly connected to the output shaft of the first motor (20), and the first motor (20) is connected to the automatic detection system signal.

8. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 7, characterized in that: The second automatic adjustment component comprises a second clamping claw (24), the second clamping claw (24) is clamped on the coarse adjustment knob (23), the side of the second clamping claw (24) away from the coarse adjustment knob (23) is coaxially fixedly connected to the output shaft of the second motor (25), and the second motor (25) is connected to the automatic detection system signal.

9. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 8, characterized in that: The inner walls of the first clamping claw (19) and the second clamping claw (24) are both provided with a flexible layer.

10. The automated Mycobacterium phenotypic drug sensitivity detection device according to claim 1, characterized in that: The automated detection system includes a receiving module, a control module, an identification module, a script module and an analysis module; The receiving module is used to receive the weight of the weighing sensor on the object carrier and control the automatic detection device through the control module, and is used to collect the spectrum information of Raman scattered light; The control module is used to control a Raman spectrometer, an electrically controlled telescopic rod, a servo motor, a first motor (20) and a second motor (25) in the automated detection device; The identification module is used to irradiate the mycobacterium species on the slide with laser light emitted by the laser head (1); The script module is used to perform background noise subtraction, baseline correction, normalization preprocessing on the obtained Raman spectral data, and filter low-quality spectra; The analysis module is used to analyze the Raman spectral data processed by the script module to obtain the vibration and rotation information of the molecules in the sample, and to derive the sensitivity of mycobacteria to phenotypic drugs through characteristic parameters such as peak position, peak intensity and peak shape in the spectrum.

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