Application of Myristicin in Pest Control

Abstract

The present invention provides an application of myristic ether in pest control, which relates to the technical field of pest control. The LC of myristic ether to locusts at 24h, 48h, 72h and 96h 50 The concentrations of myristic acid and bamboo vinegar in the water are 13299.14 μg / mL, 1500.75 μg / mL, 439.72 μg / mL and 194.57 μg / mL, respectively. The drip irrigation liquid used to treat crops after sowing and in the seedling stage can induce the crops to avoid pests. The present invention overcomes the deficiencies of the prior art, verifies that myristic acid can directly cause stomach poisoning to locusts, and has a killing effect. Myristic acid can also act on plant growth to avoid locusts and suppress their appetite, providing an important reference for the comprehensive prevention and control of locusts, and can reduce the use of chemical agents to a certain extent, reduce pollution, and comprehensively improve the prevention and control effect of locusts.

Description

Technical Field

[0001] The invention relates to the technical field of pest control, and in particular to application of myristicin in pest control. Background Art

[0002] Locusts, also known as grasshoppers, belong to the family Acrididae in the order Orthoptera and are pests that are very harmful to crops. Locusts in China are mainly divided into migratory locusts and ground locusts. Among them, migratory locusts are prone to flooding and short-term outbreaks. Migratory locusts in China mainly include the East Asian migratory locust, the Asian migratory locust and the Tibetan migratory locust, which often cause explosive disasters. Generally, the disaster lasts for a short time and causes great harm, which will cause a significant reduction in crop yields in a relatively short period of time.

[0003] The East Asian migratory locust likes to feed on plants of the Gramineae and Cyperaceae families, and mainly harms a variety of Gramineae crops such as wheat, corn, sorghum, millet, rice and broomcorn. When a large outbreak occurs, it feeds on almost all green plants. Locusts have a large food intake and can eat plants of a mass equivalent to their body weight on average every day; they have a strong reproductive capacity and can produce 300 to 400 offspring in their lifetime; the East Asian migratory locust swarm can migrate long distances and is the most destructive pest in China's history; it has a high frequency of occurrence, is highly harmful and can cause significant economic losses.

[0004] Locust control and disaster reduction is an important task for agricultural development at this stage. Traditional locust control methods are mostly to use chemical pesticides in time when locusts occur, but excessive and frequent use of chemical pesticides can easily cause locusts to develop resistance to pesticides, and to a certain extent also cause damage to the environment. In addition, locust plagues generally cause great damage in a short period of time, often from the time locusts are discovered to the time chemical pesticides are applied, locusts have already caused great damage to crops, so the overall effect of locust control at this stage is not satisfactory. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides an application of myristicin in preventing and controlling insect pests. Myristicin can directly cause stomach poisoning to locusts, thereby killing them. In addition, myristicin can also act on plant growth to repel locusts and suppress their appetite, thereby comprehensively improving the prevention and control effect of locusts.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] Application of myristic acid ether in preventing and controlling pests, wherein myristic acid ether directly kills pests or myristic acid ether induces crops to avoid pests.

[0008] Preferably, the pests are locusts.

[0009] Preferably, the crop is a grass crop.

[0010] Preferably, the LC of myristic ether to locusts at 24h, 48h, 72h, and 96h 50 They were 13299.14μg / mL, 1500.75μg / mL, 439.72μg / mL and 194.57μg / mL respectively.

[0011] Preferably, the method of using myristicin to induce crops to avoid pests is to use myristicin for drip irrigation during the crop planting period, and the crops grown thereafter have a locust-repellent effect.

[0012] A product for killing locusts, preferably containing myristicin.

[0013] A product for inducing gramineous crops to avoid locusts, the product containing 200-300 mg / L of myristic ether, 100-120 mg / L of salicylic acid, and 5-8 mL / L of bamboo vinegar.

[0014] Preferably, the method of using the product for inducing gramineous crops to avoid locusts is to perform drip irrigation once after sowing and once during the emergence stage of gramineous plants.

[0015] The present invention provides application of myristic ether in preventing and controlling insect pests, which has the advantages of:

[0016] The invention verifies the stomach poison activity of myristic acid ether to locusts, proves that myristic acid ether has a certain lethal effect on locusts at a relatively low concentration, and provides a new component selection for killing locusts. In addition, the invention adopts myristic acid ether, salicylic acid and bamboo vinegar liquid for drip irrigation treatment during crop sowing and seedling emergence, so that crops grown in the later stage have a certain repelling effect on locusts. When a locust plague occurs, the locusts selectively eat less or no crops, so that sufficient time is provided for subsequent use of insecticides to treat the locust plague, crop losses are reduced, and the control effect of locusts is comprehensively improved. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Embodiment 1:

[0018] Verification of the stomach poison activity of myristicin against locusts:

[0019] The stomach poison activity of myristicin against the third instar larvae of Locust migratoria migratoria was determined by leaf dipping method:

[0020] Five concentration gradients of 50 μg / mL, 150 μg / mL, 500 μg / mL, 1500 μg / mL, and 10000 μg / mL of myristicin were set for gastric toxicity test;

[0021] Corn leaves were cut into segments of about 3.0 cm, immersed in each concentration of compound solution for 5 s, and placed in a plastic box after natural drying. The third-instar larvae of the East Asian migratory locust with consistent development and starvation for 4 h were selected and inoculated into the box, with one larva in each box and 10 larvae in each concentration treatment, repeated 3 times, with 0.5% Tween 80 aqueous solution as negative control and pyrethroid as positive control. The survival status was checked at 1, 2, 3 and 4 days after treatment. If the insect body was stiff and unresponsive, it was judged as dead. The mortality rate and corrected mortality rate were calculated according to the following formula, and the LC50 was calculated based on the mortality rate:

[0022]

[0023] SPSS 23.0 statistical analysis software was then used to perform a one-way Anova test to obtain the mean ± standard error of myristicin and the positive control pyrethrin; a regression probability test was performed to obtain the median lethal concentration (LC) 50 , LC 50 95% confidence interval, slope ± standard error, chi-square value ( X 2 ) and P value, the specific results are shown in Table 1 below:

[0024] Table 1

[0025]

[0026] It can be seen from the above table that myristicin has a high stomach poison activity against the East Asian migratory locust and can effectively achieve the effect of killing insects. Embodiment 2:

[0027] Verify the effect of myristicin in inducing crops to avoid pests:

[0028] 1. Configure different drip irrigation fluids according to Table 2 below:

[0029] Table 2

[0030]

[0031] 2. Select corn as the experimental crop, and carry out corn sowing management on March 15, 2024 according to the conventional planting method. The sown corn is divided into 4 groups, namely D1, D2, D3, D4 and CK. Different groups are drip-irrigated once with different drip irrigation solutions 3 days after sowing (control 50mL of drip irrigation per pit), and then continue drip irrigation once during the corn seedling stage (control 200mL per plant). On July 10, the corn leaves of each group are cut for use;

[0032] 3. Verify the feeding attraction of corn leaves obtained from different groups to East Asian migratory locusts:

[0033] Ten experimental boxes were designed, and one corn leaf from each group was placed in each box (each leaf weighing 5 g). Five third-instar larvae of East Asian migratory locusts that had been starved for 4 hours were then placed in each box. The larvae were cultured in an incubator for 48 hours, and then the East Asian migratory locusts were taken out. The remaining corn leaves of each group in the experimental box were weighed and recorded as M1. The consumption rate of each group of corn leaves was calculated, where the consumption rate % = (5-M1) / 5×100%. The specific results are shown in Table 3 below:

[0034] Table 3

[0035]

[0036] According to experimental observations, the East Asian migratory locusts randomly selected food in the box at the beginning, and stopped eating soon after choosing corn leaves D1 and D2 to eat, and then looked for other leaves to eat, and were more willing to eat CK corn leaves during the whole eating process.

[0037] 4. Verify the feeding efficiency of East Asian migratory locusts on corn leaves obtained from different groups:

[0038] Five groups of experiments were designed based on different corn leaves, with three experimental boxes in each group. 5g of different corn leaves were placed in each experimental box. A third-instar larva of the East Asian migratory locust that had been starved for 4 hours was placed in each box. The experiment was observed every hour and the time it took to consume the corn leaves was recorded. The specific results are shown in Table 4 below:

[0039] Table 4

[0040]

[0041] It can be seen from the above table that the East Asian migratory locust showed obvious anorexia for the corn leaves of groups D1 and D2, and would only continue to eat when hungry. The overall consumption rate was nearly 3 times longer than that of the leaves of group CK. Combined with the choice of leaf preferences in Table 3, it can be concluded that the East Asian migratory locust would avoid choosing the corn leaves of groups D1 and D2 when it could freely choose food. For practical applications, the corn leaves of groups D1 and D2 have a certain avoidance effect on the East Asian migratory locust, and when locusts are found in agriculture, since locusts do not give priority to corn leaves of groups D1 and D2, it can give relevant personnel enough time to carry out insect control and reduce crop losses.

[0042] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. The application of myristic ether in preventing and controlling insect pests, characterized in that: The application is that myristic acid directly kills pests or myristic acid induces crops to avoid pests; the pests are locusts, and the crops are gramineous crops.

2. The use according to claim 1, characterized in that: The LC values ​​of myristic acid ether on locusts at 24 h, 48 h, 72 h, and 96 h 50 They were 13299.14μg / mL, 1500.75μg / mL, 439.72μg / mL and 194.57μg / mL respectively.

3. The use according to claim 1, characterized in that: The method of inducing crops to avoid pests with myristicin is to use myristicin for drip irrigation during the crop planting period, and the crops grown thereafter have a locust-avoiding effect.

4. A product for inducing gramineous crops to avoid locusts, characterized in that: The product contains 200-300 mg / L of myristic ether, 100-120 mg / L of salicylic acid and 5-8 mL / L of bamboo vinegar.

5. The product according to claim 4, characterized in that: The method of using the product is to perform drip irrigation once after sowing and once during the emergence stage of Gramineae plants.

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